AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009764.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009764.01.T01 MTR_2g070180 87.402 127 16 0 26 152 91 217 1.24e-79 234
MsG0280009764.01.T01 MTR_2g070140 84.677 124 19 0 26 149 75 198 8.37e-78 229
MsG0280009764.01.T01 MTR_2g070150 88.596 114 13 0 26 139 91 204 1.35e-73 219
MsG0280009764.01.T01 MTR_2g070210 72.581 124 34 0 26 149 93 216 8.10e-67 202
MsG0280009764.01.T01 MTR_2g070130 70.635 126 37 0 26 151 91 216 5.99e-66 200
MsG0280009764.01.T01 MTR_2g070200 70.543 129 35 3 26 151 91 219 1.52e-62 191
MsG0280009764.01.T01 MTR_3g467420 57.258 124 53 0 26 149 96 219 2.33e-52 166
MsG0280009764.01.T01 MTR_2g070060 54.839 124 56 0 27 150 92 215 7.14e-51 162
MsG0280009764.01.T01 MTR_3g467430 56.452 124 54 0 26 149 96 219 1.16e-49 159
MsG0280009764.01.T01 MTR_2g070070 56.452 124 54 0 27 150 92 215 9.29e-47 151
MsG0280009764.01.T01 MTR_8g056940 55.645 124 55 0 26 149 91 214 2.56e-45 147
MsG0280009764.01.T01 MTR_2g070110 55.645 124 54 1 27 150 92 214 1.56e-44 145
MsG0280009764.01.T01 MTR_5g076900 50.000 124 62 0 28 151 95 218 1.96e-42 140
MsG0280009764.01.T01 MTR_4g124130 48.413 126 65 0 24 149 90 215 6.52e-40 134
MsG0280009764.01.T01 MTR_2g070120 43.902 123 69 0 26 148 91 213 1.90e-34 120
MsG0280009764.01.T01 MTR_5g037380 32.824 131 81 3 24 147 92 222 3.52e-15 70.1
MsG0280009764.01.T01 MTR_8g087410 30.000 120 82 1 26 143 92 211 3.81e-13 64.7
MsG0280009764.01.T01 MTR_8g087425 30.159 126 86 1 27 150 92 217 1.67e-12 62.8
MsG0280009764.01.T01 MTR_7g065720 28.689 122 82 3 26 143 93 213 2.78e-12 62.4
MsG0280009764.01.T01 MTR_1g090060 32.000 125 81 3 27 147 90 214 4.02e-12 62.0
MsG0280009764.01.T01 MTR_4g043640 52.381 42 20 0 83 124 44 85 5.59e-12 58.5
MsG0280009764.01.T01 MTR_6g080440 28.926 121 82 2 26 143 89 208 6.75e-12 61.2
MsG0280009764.01.T01 MTR_7g065630 30.579 121 76 4 26 143 111 226 1.49e-11 60.5
MsG0280009764.01.T01 MTR_4g019780 31.405 121 77 4 27 143 95 213 9.40e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009764.01.T01 AT1G78380 61.789 123 47 0 27 149 92 214 7.13e-55 172
MsG0280009764.01.T01 AT1G17180 58.871 124 51 0 26 149 91 214 6.20e-54 170
MsG0280009764.01.T01 AT1G78360 60.484 124 49 0 26 149 92 215 3.19e-53 168
MsG0280009764.01.T01 AT1G78370 60.000 125 50 0 27 151 92 216 1.11e-52 166
MsG0280009764.01.T01 AT1G78320 44.920 187 65 1 1 149 1 187 2.00e-52 165
MsG0280009764.01.T01 AT1G53680 53.543 127 57 2 25 149 93 219 1.68e-47 153
MsG0280009764.01.T01 AT1G17170 54.839 124 55 1 26 149 91 213 3.63e-47 152
MsG0280009764.01.T01 AT1G78320 54.032 124 57 0 26 149 91 214 4.25e-46 150
MsG0280009764.01.T01 AT1G78340 51.613 124 60 0 26 149 91 214 6.20e-45 147
MsG0280009764.01.T01 AT3G43800 44.681 141 77 1 11 150 80 220 2.51e-42 140
MsG0280009764.01.T01 AT1G17190 45.968 124 67 0 26 149 92 215 2.11e-39 132
MsG0280009764.01.T01 AT3G09270 33.621 116 75 1 27 140 95 210 5.93e-14 67.0
MsG0280009764.01.T01 AT2G29420 29.167 120 83 1 27 144 96 215 1.80e-13 65.5
MsG0280009764.01.T01 AT2G29470 28.800 125 84 3 27 147 95 218 6.60e-13 63.9
MsG0280009764.01.T01 AT1G10360 28.906 128 84 3 27 147 94 221 2.64e-12 62.4
MsG0280009764.01.T01 AT2G29480 29.915 117 77 3 27 139 94 209 2.56e-11 59.7
MsG0280009764.01.T01 AT2G29440 33.051 118 74 3 26 139 92 208 3.50e-11 59.3
MsG0280009764.01.T01 AT2G29490 28.125 128 87 3 27 150 94 220 4.75e-11 58.9
MsG0280009764.01.T01 AT1G59700 28.906 128 84 3 27 147 96 223 9.33e-11 58.5

Find 44 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGGAGACCGGACTTATTT+TGG 0.184590 2:-57692232 MsG0280009764.01.T01:CDS
GACATCTATGAATTCTTTCT+TGG 0.270245 2:+57692276 None:intergenic
CAAATTGTTGGAGCAAGAAT+TGG 0.293479 2:-57692251 MsG0280009764.01.T01:CDS
AAGTGATTCTGCTTGATTCC+TGG 0.317076 2:-57692855 MsG0280009764.01.T01:CDS
TTTAAAAGCTATGAGACCTT+TGG 0.358405 2:-57692157 MsG0280009764.01.T01:CDS
AAGGAACCTTTGGGCCAAAA+AGG 0.360471 2:-57692317 MsG0280009764.01.T01:CDS
GATTCCTGGGCAAGTCCATT+TGG 0.366880 2:-57692841 MsG0280009764.01.T01:CDS
CATAGATGTCCTCAAATTGT+TGG 0.381980 2:-57692263 MsG0280009764.01.T01:CDS
ATGAAGGTGGAAGGAACCTT+TGG 0.390594 2:-57692327 MsG0280009764.01.T01:CDS
CACTTATTCCATTCTACACT+TGG 0.395366 2:-57692180 MsG0280009764.01.T01:CDS
ATTCTTGCTCCAACAATTTG+AGG 0.395572 2:+57692254 None:intergenic
TCATGGACCTTATCCTGATC+AGG 0.413575 2:+57692049 None:intergenic
CTCAATCTGCATACATCTCT+TGG 0.415648 2:+57692090 None:intergenic
AGAGATCACAAGCTAGGTTC+TGG 0.428783 2:-57692600 MsG0280009764.01.T01:CDS
TTGGACCAAGCAATGAACTT+AGG 0.437629 2:+57692109 None:intergenic
TTCAAAGTCACTCCCTGATC+AGG 0.452471 2:-57692062 MsG0280009764.01.T01:CDS
TGGACCAAGCAATGAACTTA+GGG 0.476815 2:+57692110 None:intergenic
AAATTGTTGGAGCAAGAATT+GGG 0.479522 2:-57692250 MsG0280009764.01.T01:CDS
AGTGATTCTGCTTGATTCCT+GGG 0.480041 2:-57692854 MsG0280009764.01.T01:CDS
GCTTGATACCCTTTCAGCTA+GGG 0.481798 2:+57692801 None:intergenic
TGCTTGATACCCTTTCAGCT+AGG 0.489033 2:+57692800 None:intergenic
CTCTAAATTGATGTTTCCAA+AGG 0.489801 2:+57692141 None:intergenic
GAGATCACAAGCTAGGTTCT+GGG 0.491244 2:-57692599 MsG0280009764.01.T01:CDS
CAGAATAGCCCTAGCTGAAA+GGG 0.493572 2:-57692809 MsG0280009764.01.T01:intron
AATGCCCTAAGTTCATTGCT+TGG 0.495645 2:-57692114 MsG0280009764.01.T01:CDS
GTCACTCCCTGATCAGGATA+AGG 0.500395 2:-57692056 MsG0280009764.01.T01:CDS
GGCTGATTATGTTGATAAGA+AGG 0.507102 2:-57692578 MsG0280009764.01.T01:intron
TGAAGGTGGAAGGAACCTTT+GGG 0.507522 2:-57692326 MsG0280009764.01.T01:CDS
ATTCTGAGTCTCATGCCAAA+TGG 0.515676 2:+57692826 None:intergenic
TGGAAACATCAATTTAGAGA+AGG 0.525535 2:-57692137 MsG0280009764.01.T01:CDS
AATGTCAAATAGATATATGA+AGG 0.531559 2:-57692343 MsG0280009764.01.T01:intron
ATTGTAGAGCTGAGGAAGAC+GGG 0.532079 2:-57692022 MsG0280009764.01.T01:CDS
CATGCCAAATGGACTTGCCC+AGG 0.533387 2:+57692837 None:intergenic
TATCTCCTCCAAAATAAGTC+CGG 0.546574 2:+57692224 None:intergenic
TCAGAATAGCCCTAGCTGAA+AGG 0.549650 2:-57692810 MsG0280009764.01.T01:CDS
CTTATCAGAGATCACAAGCT+AGG 0.551577 2:-57692606 MsG0280009764.01.T01:intron
TGGAGCAAGAATTGGGAGAC+CGG 0.556673 2:-57692243 MsG0280009764.01.T01:CDS
CATTGTAGAGCTGAGGAAGA+CGG 0.561187 2:-57692023 MsG0280009764.01.T01:CDS
AATAGATATATGAAGGTGGA+AGG 0.568115 2:-57692336 MsG0280009764.01.T01:intron
CAGAATCACTTCATCAGCCA+TGG 0.592382 2:+57692867 None:intergenic
TCAGCTCTACAATGTAGTCA+TGG 0.600557 2:+57692032 None:intergenic
CATGGACCTTATCCTGATCA+GGG 0.606504 2:+57692050 None:intergenic
GTCAAATAGATATATGAAGG+TGG 0.608108 2:-57692340 MsG0280009764.01.T01:intron
ATGACTACATTGTAGAGCTG+AGG 0.637957 2:-57692030 MsG0280009764.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATAAAATAAAGTTTAA+CGG + Chr2:57692418-57692437 None:intergenic 10.0%
!! AATGTCAAATAGATATATGA+AGG - Chr2:57692537-57692556 MsG0280009764.01.T01:intron 20.0%
!! TAGTTTATATGAGACTTATA+TGG - Chr2:57692458-57692477 MsG0280009764.01.T01:intron 20.0%
AAATTGTTGGAGCAAGAATT+GGG - Chr2:57692630-57692649 MsG0280009764.01.T01:intron 30.0%
AATAGATATATGAAGGTGGA+AGG - Chr2:57692544-57692563 MsG0280009764.01.T01:intron 30.0%
AGCAACTAAAGATTCACAAT+AGG + Chr2:57692198-57692217 None:intergenic 30.0%
CTCTAAATTGATGTTTCCAA+AGG + Chr2:57692742-57692761 None:intergenic 30.0%
GACATCTATGAATTCTTTCT+TGG + Chr2:57692607-57692626 None:intergenic 30.0%
GTCAAATAGATATATGAAGG+TGG - Chr2:57692540-57692559 MsG0280009764.01.T01:intron 30.0%
TGGAAACATCAATTTAGAGA+AGG - Chr2:57692743-57692762 MsG0280009764.01.T01:intron 30.0%
TTCAGTATATGATTGAGGTT+TGG - Chr2:57692219-57692238 MsG0280009764.01.T01:CDS 30.0%
TTTAAAAGCTATGAGACCTT+TGG - Chr2:57692723-57692742 MsG0280009764.01.T01:intron 30.0%
! CTTTTAAACCAAGTGTAGAA+TGG + Chr2:57692711-57692730 None:intergenic 30.0%
AGGGTCATTTGTAACAACAA+GGG + Chr2:57692137-57692156 None:intergenic 35.0%
ATCCCTGTTCTGATTCATAA+CGG - Chr2:57692168-57692187 MsG0280009764.01.T01:CDS 35.0%
ATTCTTGCTCCAACAATTTG+AGG + Chr2:57692629-57692648 None:intergenic 35.0%
CAAATTGTTGGAGCAAGAAT+TGG - Chr2:57692629-57692648 MsG0280009764.01.T01:intron 35.0%
CACTTATTCCATTCTACACT+TGG - Chr2:57692700-57692719 MsG0280009764.01.T01:intron 35.0%
CATAGATGTCCTCAAATTGT+TGG - Chr2:57692617-57692636 MsG0280009764.01.T01:intron 35.0%
TAAGGATCAGAAGGCAAAAA+AGG + Chr2:57692258-57692277 None:intergenic 35.0%
TACCGTTATGAATCAGAACA+GGG + Chr2:57692173-57692192 None:intergenic 35.0%
TATCTCCTCCAAAATAAGTC+CGG + Chr2:57692659-57692678 None:intergenic 35.0%
TGCTGTTCAGTATATGATTG+AGG - Chr2:57692214-57692233 MsG0280009764.01.T01:CDS 35.0%
TTACCGTTATGAATCAGAAC+AGG + Chr2:57692174-57692193 None:intergenic 35.0%
! GGCTGATTATGTTGATAAGA+AGG - Chr2:57692302-57692321 MsG0280009764.01.T01:CDS 35.0%
!! TATTTTGGAGGAGATAAGCT+TGG - Chr2:57692663-57692682 MsG0280009764.01.T01:intron 35.0%
AACAGGGATTTCTTGTGAAC+AGG + Chr2:57692157-57692176 None:intergenic 40.0%
AAGTGATTCTGCTTGATTCC+TGG - Chr2:57692025-57692044 MsG0280009764.01.T01:CDS 40.0%
AATGCCCTAAGTTCATTGCT+TGG - Chr2:57692766-57692785 MsG0280009764.01.T01:intron 40.0%
ACAGGGATTTCTTGTGAACA+GGG + Chr2:57692156-57692175 None:intergenic 40.0%
AGTGATTCTGCTTGATTCCT+GGG - Chr2:57692026-57692045 MsG0280009764.01.T01:CDS 40.0%
ATGACTACATTGTAGAGCTG+AGG - Chr2:57692850-57692869 MsG0280009764.01.T01:CDS 40.0%
ATTCTGAGTCTCATGCCAAA+TGG + Chr2:57692057-57692076 None:intergenic 40.0%
CAGGGTCATTTGTAACAACA+AGG + Chr2:57692138-57692157 None:intergenic 40.0%
CTCAATCTGCATACATCTCT+TGG + Chr2:57692793-57692812 None:intergenic 40.0%
CTTATCAGAGATCACAAGCT+AGG - Chr2:57692274-57692293 MsG0280009764.01.T01:CDS 40.0%
GATCTCTGATAAGGATCAGA+AGG + Chr2:57692267-57692286 None:intergenic 40.0%
TCAGCTCTACAATGTAGTCA+TGG + Chr2:57692851-57692870 None:intergenic 40.0%
TGGACCAAGCAATGAACTTA+GGG + Chr2:57692773-57692792 None:intergenic 40.0%
TTGGACCAAGCAATGAACTT+AGG + Chr2:57692774-57692793 None:intergenic 40.0%
! CTAGCTTGTGATCTCTGATA+AGG + Chr2:57692276-57692295 None:intergenic 40.0%
! TTCTTGCTCTTCTCCCTTTT+TGG + Chr2:57692580-57692599 None:intergenic 40.0%
AAGGAACCTTTGGGCCAAAA+AGG - Chr2:57692563-57692582 MsG0280009764.01.T01:intron 45.0%
AGAGATCACAAGCTAGGTTC+TGG - Chr2:57692280-57692299 MsG0280009764.01.T01:CDS 45.0%
AGGAACCTTTGGGCCAAAAA+GGG - Chr2:57692564-57692583 MsG0280009764.01.T01:intron 45.0%
ATGAAGGTGGAAGGAACCTT+TGG - Chr2:57692553-57692572 MsG0280009764.01.T01:intron 45.0%
ATTGTAGAGCTGAGGAAGAC+GGG - Chr2:57692858-57692877 MsG0280009764.01.T01:CDS 45.0%
CAGAATAGCCCTAGCTGAAA+GGG - Chr2:57692071-57692090 MsG0280009764.01.T01:CDS 45.0%
CATGGACCTTATCCTGATCA+GGG + Chr2:57692833-57692852 None:intergenic 45.0%
CATTGTAGAGCTGAGGAAGA+CGG - Chr2:57692857-57692876 MsG0280009764.01.T01:CDS 45.0%
GAGATCACAAGCTAGGTTCT+GGG - Chr2:57692281-57692300 MsG0280009764.01.T01:CDS 45.0%
GCTTGATACCCTTTCAGCTA+GGG + Chr2:57692082-57692101 None:intergenic 45.0%
TCAGAATAGCCCTAGCTGAA+AGG - Chr2:57692070-57692089 MsG0280009764.01.T01:CDS 45.0%
TCATGGACCTTATCCTGATC+AGG + Chr2:57692834-57692853 None:intergenic 45.0%
TGAAGGTGGAAGGAACCTTT+GGG - Chr2:57692554-57692573 MsG0280009764.01.T01:intron 45.0%
TGCTTGATACCCTTTCAGCT+AGG + Chr2:57692083-57692102 None:intergenic 45.0%
TTCAAAGTCACTCCCTGATC+AGG - Chr2:57692818-57692837 MsG0280009764.01.T01:CDS 45.0%
! TTGGGAGACCGGACTTATTT+TGG - Chr2:57692648-57692667 MsG0280009764.01.T01:intron 45.0%
GATTCCTGGGCAAGTCCATT+TGG - Chr2:57692039-57692058 MsG0280009764.01.T01:CDS 50.0%
GTCACTCCCTGATCAGGATA+AGG - Chr2:57692824-57692843 MsG0280009764.01.T01:CDS 50.0%
TGGAGCAAGAATTGGGAGAC+CGG - Chr2:57692637-57692656 MsG0280009764.01.T01:intron 50.0%
!! CTTCTCCCTTTTTGGCCCAA+AGG + Chr2:57692572-57692591 None:intergenic 50.0%
!!! GGAGACCGGACTTATTTTGG+AGG - Chr2:57692651-57692670 MsG0280009764.01.T01:intron 50.0%
! CATGCCAAATGGACTTGCCC+AGG + Chr2:57692046-57692065 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 57692015 57692887 57692015 ID=MsG0280009764.01;Name=MsG0280009764.01
Chr2 mRNA 57692015 57692887 57692015 ID=MsG0280009764.01.T01;Parent=MsG0280009764.01;Name=MsG0280009764.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|1|3|0|152
Chr2 exon 57692810 57692887 57692810 ID=MsG0280009764.01.T01:exon:6151;Parent=MsG0280009764.01.T01
Chr2 exon 57692579 57692620 57692579 ID=MsG0280009764.01.T01:exon:6150;Parent=MsG0280009764.01.T01
Chr2 exon 57692015 57692353 57692015 ID=MsG0280009764.01.T01:exon:6149;Parent=MsG0280009764.01.T01
Chr2 CDS 57692810 57692887 57692810 ID=MsG0280009764.01.T01:cds;Parent=MsG0280009764.01.T01
Chr2 CDS 57692579 57692620 57692579 ID=MsG0280009764.01.T01:cds;Parent=MsG0280009764.01.T01
Chr2 CDS 57692015 57692353 57692015 ID=MsG0280009764.01.T01:cds;Parent=MsG0280009764.01.T01
Gene Sequence

>MsG0280009764.01.T01

ATGGCTGATGAAGTGATTCTGCTTGATTCCTGGGCAAGTCCATTTGGCATGAGACTCAGAATAGCCCTAGCTGAAAGGAGATCACAAGCTAGGTTCTGGGCTGATTATGTTGATAAGAAGATATATGAAGGTGGAAGGAACCTTTGGGCCAAAAAGGGAGAAGAGCAAGAAGTTGCCAAGAAAGAATTCATAGATGTCCTCAAATTGTTGGAGCAAGAATTGGGAGACCGGACTTATTTTGGAGGAGATAAGCTTGGTTTTGTTGATGTAGCACTTATTCCATTCTACACTTGGTTTAAAAGCTATGAGACCTTTGGAAACATCAATTTAGAGAAGGAATGCCCTAAGTTCATTGCTTGGTCCAAGAGATGTATGCAGATTGAGAGTGTTTCAAAGTCACTCCCTGATCAGGATAAGGTCCATGACTACATTGTAGAGCTGAGGAAGACGGGTTATTGA

Protein sequence

>MsG0280009764.01.T01

MADEVILLDSWASPFGMRLRIALAERRSQARFWADYVDKKIYEGGRNLWAKKGEEQEVAKKEFIDVLKLLEQELGDRTYFGGDKLGFVDVALIPFYTWFKSYETFGNINLEKECPKFIAWSKRCMQIESVSKSLPDQDKVHDYIVELRKTGY*