AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009902.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009902.01.T01 MTR_2g073070 66.337 101 2 2 15 83 35 135 1.54e-30 108
MsG0280009902.01.T01 MTR_2g073190 58.511 94 11 1 15 80 476 569 1.86e-23 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009902.01.T01 AT1G78560 55.000 100 13 2 15 82 196 295 1.66e-22 90.1

Find 55 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGTTCTCGGCGTAGTTTC+TGG 0.186694 2:-60431156 MsG0280009902.01.T01:three_prime_UTR
GTCTGCCTTCTTCACGCTTC+TGG 0.218304 2:-60431389 MsG0280009902.01.T01:three_prime_UTR
CTCTATCTTTGGTAGCATCT+TGG 0.304708 2:-60431075 MsG0280009902.01.T01:three_prime_UTR
CAGCACAAGGTACTGTTGTT+AGG 0.306098 2:+60431111 None:intergenic
TCAAGTTGGTCCTCTATCTT+TGG 0.306289 2:-60431086 MsG0280009902.01.T01:three_prime_UTR
ATGTTCTTGCCAGGAGTCTT+GGG 0.355741 2:-60431352 MsG0280009902.01.T01:three_prime_UTR
TATGTTCTTGCCAGGAGTCT+TGG 0.376216 2:-60431353 MsG0280009902.01.T01:three_prime_UTR
CATGCCAACTTCAAGGGATA+TGG 0.395530 2:+60431307 None:intergenic
TATATTCAGTCCTTCTTCTC+AGG 0.415376 2:+60431027 None:intergenic
ATTGGGTTGACTACTGGTAT+TGG 0.416878 2:+60434049 None:intergenic
GACAGCTGCTAGCACTCTTT+CGG 0.428700 2:-60432500 MsG0280009902.01.T01:CDS
ATCCTTATGTCCTGGTGGAC+AGG 0.433039 2:-60431424 MsG0280009902.01.T01:CDS
CACATTAAAATTGCCACAGT+TGG 0.434685 2:+60431475 None:intergenic
AGCACAAGGTACTGTTGTTA+GGG 0.436933 2:+60431112 None:intergenic
TGCTAGCACTCTTTCGGCTG+TGG 0.444821 2:-60432494 MsG0280009902.01.T01:intron
ACAGCTAAGGGTGGCATCAA+TGG 0.447547 2:+60431500 None:intergenic
TTACCTGCATGCCAACTTCA+AGG 0.452000 2:+60431300 None:intergenic
TACCTGTCCACCAGGACATA+AGG 0.452251 2:+60431422 None:intergenic
TCTTCGGATATGTTCTTGCC+AGG 0.460082 2:-60431361 MsG0280009902.01.T01:three_prime_UTR
CTTGAGATGAGAACAATTCA+AGG 0.465542 2:-60434079 None:intergenic
GCCACAGTTGGCTACAGCTA+AGG 0.470276 2:+60431487 None:intergenic
TCTTCTGCATCCTTATGTCC+TGG 0.472092 2:-60431432 MsG0280009902.01.T01:CDS
TCTTGCCAGGAGTCTTGGGC+TGG 0.474396 2:-60431348 MsG0280009902.01.T01:three_prime_UTR
ATCCCTTGAAGTTGGCATGC+AGG 0.474488 2:-60431303 MsG0280009902.01.T01:intron
TTTCTGGCTGCCAAGCACTT+TGG 0.479339 2:-60431140 MsG0280009902.01.T01:three_prime_UTR
ATCTTTGGTAGCATCTTGGC+TGG 0.480333 2:-60431071 MsG0280009902.01.T01:three_prime_UTR
AGAAGTGCACCAGCCAATAC+AGG 0.481082 2:+60431560 None:intergenic
TAACCCATTGGGTTGACTAC+TGG 0.481418 2:+60434043 None:intergenic
ATATTGTAGAACTCAGTTCT+CGG 0.500688 2:-60431168 MsG0280009902.01.T01:intron
AACATCCAGCCCAAGACTCC+TGG 0.501138 2:+60431343 None:intergenic
ACATCATAGTTACATGCAAA+AGG 0.502992 2:+60430992 None:intergenic
ACACAAGTTTAACAAGAGGT+TGG 0.513689 2:+60431525 None:intergenic
GTAGCATCTTGGCTGGTATG+TGG 0.518881 2:-60431064 MsG0280009902.01.T01:three_prime_UTR
ACGTCCCTATATAACCCATT+GGG 0.524262 2:+60434032 None:intergenic
CAAGATGCTACCAAAGATAG+AGG 0.526602 2:+60431076 None:intergenic
TAGGGGAATTCCAAAGTGCT+TGG 0.528175 2:+60431130 None:intergenic
GCTCTGTCCTCCTGAGAAGA+AGG 0.530930 2:-60431037 MsG0280009902.01.T01:three_prime_UTR
CGGACCATATCCCTTGAAGT+TGG 0.531221 2:-60431311 MsG0280009902.01.T01:three_prime_UTR
GAAGGACTGAATATACTAGC+TGG 0.537237 2:-60431019 MsG0280009902.01.T01:three_prime_UTR
CCACAGTTGGCTACAGCTAA+GGG 0.543457 2:+60431488 None:intergenic
TACCTTGTGCTGTTTCAAGT+TGG 0.549735 2:-60431100 MsG0280009902.01.T01:three_prime_UTR
ATACCAGTAGTCAACCCAAT+GGG 0.578427 2:-60434046 MsG0280009902.01.T01:intron
TACCTGCATGCCAACTTCAA+GGG 0.586149 2:+60431301 None:intergenic
TCTAAGAATACCTGTCCACC+AGG 0.594029 2:+60431414 None:intergenic
ATTCAGTCCTTCTTCTCAGG+AGG 0.597567 2:+60431030 None:intergenic
TCTGCATCCTTATGTCCTGG+TGG 0.609572 2:-60431429 MsG0280009902.01.T01:CDS
GGAGACACAAGTTTAACAAG+AGG 0.618474 2:+60431521 None:intergenic
CCCTTAGCTGTAGCCAACTG+TGG 0.627786 2:-60431488 MsG0280009902.01.T01:CDS
ATATTGTAACATATCTTGCA+CGG 0.648486 2:-60432630 MsG0280009902.01.T01:intron
GGCTGGATGTTGCATCATCG+CGG 0.650097 2:-60431331 MsG0280009902.01.T01:three_prime_UTR
AATACCAGTAGTCAACCCAA+TGG 0.653830 2:-60434047 MsG0280009902.01.T01:CDS
GCACAAGGTACTGTTGTTAG+GGG 0.695410 2:+60431113 None:intergenic
CAGTTGGCTACAGCTAAGGG+TGG 0.696593 2:+60431491 None:intergenic
GCTAGCAGCTGTCATTACAA+CGG 0.699177 2:+60432510 None:intergenic
GACCAACTTGAAACAGCACA+AGG 0.708639 2:+60431098 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATAGTTAATAAATATAAC+AGG + Chr2:60431576-60431595 None:intergenic 15.0%
!!! ATTAATCAAAATTTTGAATG+TGG - Chr2:60431353-60431372 MsG0280009902.01.T01:three_prime_UTR 15.0%
!!! GTAAGTAGTTATTTTTTTTT+TGG - Chr2:60431006-60431025 MsG0280009902.01.T01:three_prime_UTR 15.0%
!!! TCTATTAATAAAGTATTCAT+TGG + Chr2:60432418-60432437 None:intergenic 15.0%
!!! TTTAATTTTTCTGCTTTATT+CGG - Chr2:60432821-60432840 MsG0280009902.01.T01:intron 15.0%
!!! TTTTGAAATTGTAAAAGAAA+TGG + Chr2:60433165-60433184 None:intergenic 15.0%
!!! TTTTGATTAATTGTGTTTAA+TGG + Chr2:60431344-60431363 None:intergenic 15.0%
!! AAACAAAGACTACATAAATT+AGG + Chr2:60432337-60432356 None:intergenic 20.0%
!! AATTATACATTCAAAGATTG+TGG + Chr2:60431383-60431402 None:intergenic 20.0%
!! ATTTCTAATTCAATTTGGAT+TGG - Chr2:60431215-60431234 MsG0280009902.01.T01:intron 20.0%
!! CAACAATTTCTAATTCAATT+TGG - Chr2:60431210-60431229 MsG0280009902.01.T01:intron 20.0%
!! GTTAATGTAGTTTAAATACA+TGG - Chr2:60431260-60431279 MsG0280009902.01.T01:intron 20.0%
!! TAATTTATGTAGTCTTTGTT+TGG - Chr2:60432336-60432355 MsG0280009902.01.T01:intron 20.0%
!! TATTCTATATAGTCTTTGTT+TGG - Chr2:60432255-60432274 MsG0280009902.01.T01:intron 20.0%
!!! AAACAATGAAAACAATGTTT+TGG + Chr2:60433220-60433239 None:intergenic 20.0%
!!! ATATTTTGAACGTTGATTAA+TGG - Chr2:60431457-60431476 MsG0280009902.01.T01:CDS 20.0%
!!! ATTAATGACGTTTAAAATTG+TGG - Chr2:60431513-60431532 MsG0280009902.01.T01:CDS 20.0%
!!! GGAAAAAACAAATCATTTTA+TGG + Chr2:60431705-60431724 None:intergenic 20.0%
!!! TAAACTTTTCATACTGTTTA+TGG - Chr2:60432795-60432814 MsG0280009902.01.T01:intron 20.0%
!!! TTCACAATTCTTTTTTATCA+AGG + Chr2:60431864-60431883 None:intergenic 20.0%
! AGACTACATAAATTAGGTTA+AGG + Chr2:60432331-60432350 None:intergenic 25.0%
! ATAATGCTTTAGTCCTATAT+TGG - Chr2:60432549-60432568 MsG0280009902.01.T01:intron 25.0%
! ATATTGTAACATATCTTGCA+CGG - Chr2:60432377-60432396 MsG0280009902.01.T01:intron 25.0%
! ATTAATAGATTTGATGAGCA+GGG - Chr2:60432429-60432448 MsG0280009902.01.T01:intron 25.0%
! ATTTAACAATTAGTAGAGTC+AGG - Chr2:60431062-60431081 MsG0280009902.01.T01:three_prime_UTR 25.0%
! CTACATAAGATAGATGTTAA+TGG + Chr2:60432177-60432196 None:intergenic 25.0%
! CTTACAAAAAATGCAGATAA+TGG + Chr2:60432072-60432091 None:intergenic 25.0%
! GACTCTACTAATTGTTAAAT+TGG + Chr2:60431062-60431081 None:intergenic 25.0%
! GTATAATTGAACACAAATTG+TGG - Chr2:60431395-60431414 MsG0280009902.01.T01:three_prime_UTR 25.0%
! TATGGAAATTCATAATCACA+GGG - Chr2:60431090-60431109 MsG0280009902.01.T01:three_prime_UTR 25.0%
! TATTAATAGATTTGATGAGC+AGG - Chr2:60432428-60432447 MsG0280009902.01.T01:intron 25.0%
! TTATGGAAATTCATAATCAC+AGG - Chr2:60431089-60431108 MsG0280009902.01.T01:three_prime_UTR 25.0%
! TTTAACAATTAGTAGAGTCA+GGG - Chr2:60431063-60431082 MsG0280009902.01.T01:three_prime_UTR 25.0%
!!! ACAATGTTGATGACAAAATA+TGG - Chr2:60432588-60432607 MsG0280009902.01.T01:intron 25.0%
!!! TGATCTATTTATTTTGCTAG+TGG - Chr2:60432460-60432479 MsG0280009902.01.T01:intron 25.0%
!!! TTTGTTATTCTGACACAAAT+TGG - Chr2:60431186-60431205 MsG0280009902.01.T01:intron 25.0%
AAAAGGGTAAAAAAGAACCT+TGG - Chr2:60432872-60432891 MsG0280009902.01.T01:intron 30.0%
AAAGGGTAAAAAAGAACCTT+GGG - Chr2:60432873-60432892 MsG0280009902.01.T01:intron 30.0%
AACATATTTACCAGCTAGTT+TGG + Chr2:60433024-60433043 None:intergenic 30.0%
AATATCGAGGGTAAAGAAAA+GGG + Chr2:60433824-60433843 None:intergenic 30.0%
ACATCATAGTTACATGCAAA+AGG + Chr2:60434018-60434037 None:intergenic 30.0%
AGAAAACGGAGATACAATTA+GGG + Chr2:60433407-60433426 None:intergenic 30.0%
ATATTGTAGAACTCAGTTCT+CGG - Chr2:60433839-60433858 MsG0280009902.01.T01:intron 30.0%
ATCAACATTGTACATCAAGT+TGG + Chr2:60432579-60432598 None:intergenic 30.0%
CAATGAGTAAACCACAAAAT+TGG + Chr2:60431148-60431167 None:intergenic 30.0%
CTTATTAATATCGACATTGC+AGG - Chr2:60433061-60433080 MsG0280009902.01.T01:intron 30.0%
GAAAATGAAAACAAGAACCA+TGG + Chr2:60433094-60433113 None:intergenic 30.0%
GAATTACAGTCAAATCGAAA+TGG - Chr2:60432620-60432639 MsG0280009902.01.T01:intron 30.0%
GACTATATAGAATACGTTAC+AGG + Chr2:60432249-60432268 None:intergenic 30.0%
GGATATTAATGGGATATAAC+AGG - Chr2:60431298-60431317 MsG0280009902.01.T01:intron 30.0%
GTATCCATACACAAATACAA+GGG - Chr2:60431785-60431804 MsG0280009902.01.T01:intron 30.0%
TGCAAAAAAGAGAATATGCA+AGG + Chr2:60433268-60433287 None:intergenic 30.0%
TGTATCCATACACAAATACA+AGG - Chr2:60431784-60431803 MsG0280009902.01.T01:intron 30.0%
TTCCAAAGAGATAACACAAA+AGG - Chr2:60431721-60431740 MsG0280009902.01.T01:intron 30.0%
TTGTATTTGTGTATGGATAC+AGG + Chr2:60431785-60431804 None:intergenic 30.0%
TTGTGGTTAATTACGTTCAT+TGG - Chr2:60431530-60431549 MsG0280009902.01.T01:CDS 30.0%
TTGTTTGCAGAATTGATCAA+AGG + Chr2:60433798-60433817 None:intergenic 30.0%
! AACCTTTTGTGTTATCTCTT+TGG + Chr2:60431726-60431745 None:intergenic 30.0%
! CAGCTGGTTTAATATTAGTT+GGG - Chr2:60432121-60432140 MsG0280009902.01.T01:intron 30.0%
! CTTTATTCGGCACTTTAAAA+TGG - Chr2:60432834-60432853 MsG0280009902.01.T01:intron 30.0%
! GTCATCACAATTTTAAGCAT+AGG - Chr2:60433741-60433760 MsG0280009902.01.T01:intron 30.0%
! TGTGTTTAGCGGATATTAAT+GGG - Chr2:60431288-60431307 MsG0280009902.01.T01:intron 30.0%
!! TATCTTAGATAATGGAGTGA+TGG + Chr2:60432747-60432766 None:intergenic 30.0%
!! TCAAGTTGGAAAACCAATAT+AGG + Chr2:60432565-60432584 None:intergenic 30.0%
!! TGTCAGATTTAGTAGTTTTG+AGG - Chr2:60432707-60432726 MsG0280009902.01.T01:intron 30.0%
!!! CTAGTTGATAAACCTTTTCA+CGG - Chr2:60433771-60433790 MsG0280009902.01.T01:intron 30.0%
AACTGAGTTCTACAATATCG+AGG + Chr2:60433837-60433856 None:intergenic 35.0%
AAGGGTAAAAAAGAACCTTG+GGG - Chr2:60432874-60432893 MsG0280009902.01.T01:intron 35.0%
ACACAAGTTTAACAAGAGGT+TGG + Chr2:60433485-60433504 None:intergenic 35.0%
ACATGGATAACTGTGTTTAG+CGG - Chr2:60431277-60431296 MsG0280009902.01.T01:intron 35.0%
ACGGTAGGTATCTTAGATAA+TGG + Chr2:60432755-60432774 None:intergenic 35.0%
ACTGAGTTCTACAATATCGA+GGG + Chr2:60433836-60433855 None:intergenic 35.0%
CAATATCGAGGGTAAAGAAA+AGG + Chr2:60433825-60433844 None:intergenic 35.0%
CACATTAAAATTGCCACAGT+TGG + Chr2:60433535-60433554 None:intergenic 35.0%
CACATTTGGTATTATGTAGC+TGG - Chr2:60432284-60432303 MsG0280009902.01.T01:intron 35.0%
CTATCTTATGTAGTCTGTGT+TGG - Chr2:60432184-60432203 MsG0280009902.01.T01:intron 35.0%
GAGAAAACGGAGATACAATT+AGG + Chr2:60433408-60433427 None:intergenic 35.0%
GGATGACAATGATATTGTCA+AGG - Chr2:60431806-60431825 MsG0280009902.01.T01:intron 35.0%
TAAATCACCAACACAATGTG+AGG + Chr2:60432213-60432232 None:intergenic 35.0%
TATATTCAGTCCTTCTTCTC+AGG + Chr2:60433983-60434002 None:intergenic 35.0%
TCATCCCTTGTATTTGTGTA+TGG + Chr2:60431792-60431811 None:intergenic 35.0%
TGTATGGATACAGGTGAAAA+TGG + Chr2:60431776-60431795 None:intergenic 35.0%
TTGGAATCTCACAATATCGA+AGG + Chr2:60431968-60431987 None:intergenic 35.0%
! CTGTGTTTAGCGGATATTAA+TGG - Chr2:60431287-60431306 MsG0280009902.01.T01:intron 35.0%
! GCAATCTCAGCAGATTTTTA+CGG + Chr2:60432774-60432793 None:intergenic 35.0%
! GCAGCTGGTTTAATATTAGT+TGG - Chr2:60432120-60432139 MsG0280009902.01.T01:intron 35.0%
! TAAAATGGCTACGAAGTATC+AGG - Chr2:60432849-60432868 MsG0280009902.01.T01:intron 35.0%
! TGTTCGCTTGTAACATTTTC+TGG - Chr2:60434043-60434062 MsG0280009902.01.T01:intron 35.0%
! TTGATGACAAAATATGGTGC+TGG - Chr2:60432594-60432613 MsG0280009902.01.T01:intron 35.0%
!! AAAGTTTGGGACCAATTTTG+TGG - Chr2:60431134-60431153 MsG0280009902.01.T01:three_prime_UTR 35.0%
!! CAACTGTGGCAATTTTAATG+TGG - Chr2:60433533-60433552 MsG0280009902.01.T01:intron 35.0%
!! TCAACCCAATGGGTTATATA+GGG - Chr2:60430971-60430990 MsG0280009902.01.T01:three_prime_UTR 35.0%
!! TTATTTTGCTAGTGGAAACG+TGG - Chr2:60432468-60432487 MsG0280009902.01.T01:intron 35.0%
AAAAAAGAACCTTGGGGGAA+AGG - Chr2:60432880-60432899 MsG0280009902.01.T01:intron 40.0%
AAAAAGAACCTTGGGGGAAA+GGG - Chr2:60432881-60432900 MsG0280009902.01.T01:intron 40.0%
AAACCAGCTGCATAATGTGA+TGG + Chr2:60432111-60432130 None:intergenic 40.0%
AATACCAGTAGTCAACCCAA+TGG - Chr2:60430960-60430979 MsG0280009902.01.T01:three_prime_UTR 40.0%
ACATTGCAGGTTAACTTCCA+TGG - Chr2:60433074-60433093 MsG0280009902.01.T01:intron 40.0%
ACCACTTAGAGTTAACAACG+TGG + Chr2:60432686-60432705 None:intergenic 40.0%
ACGTCCCTATATAACCCATT+GGG + Chr2:60430978-60430997 None:intergenic 40.0%
AGGGTAAAAAAGAACCTTGG+GGG - Chr2:60432875-60432894 MsG0280009902.01.T01:intron 40.0%
AGTTAATGCAACTGCGATTG+TGG - Chr2:60431891-60431910 MsG0280009902.01.T01:intron 40.0%
ATACCAGTAGTCAACCCAAT+GGG - Chr2:60430961-60430980 MsG0280009902.01.T01:three_prime_UTR 40.0%
ATGCAAACTCAGATGCTGTT+TGG + Chr2:60431987-60432006 None:intergenic 40.0%
CAAGATGCTACCAAAGATAG+AGG + Chr2:60433934-60433953 None:intergenic 40.0%
CACAACCTGTTGAGAGAAAA+CGG + Chr2:60433421-60433440 None:intergenic 40.0%
CACCTGAAAATTGTCAGAAC+AGG + Chr2:60432046-60432065 None:intergenic 40.0%
CTCGCATTACGAAATCCATA+TGG + Chr2:60432648-60432667 None:intergenic 40.0%
GAAGGACTGAATATACTAGC+TGG - Chr2:60433988-60434007 MsG0280009902.01.T01:intron 40.0%
GAGTGTGTCGTACAAAGTTT+GGG - Chr2:60431121-60431140 MsG0280009902.01.T01:three_prime_UTR 40.0%
GGAGACACAAGTTTAACAAG+AGG + Chr2:60433489-60433508 None:intergenic 40.0%
GTCCTAAAATGCTGATGTTG+AGG - Chr2:60431925-60431944 MsG0280009902.01.T01:intron 40.0%
TACGTCCCTATATAACCCAT+TGG + Chr2:60430979-60430998 None:intergenic 40.0%
TCCACGTTGTTAACTCTAAG+TGG - Chr2:60432682-60432701 MsG0280009902.01.T01:intron 40.0%
TGAGTGTGTCGTACAAAGTT+TGG - Chr2:60431120-60431139 MsG0280009902.01.T01:three_prime_UTR 40.0%
TTGATCAAAGGACCGTGAAA+AGG + Chr2:60433786-60433805 None:intergenic 40.0%
TTGTTTGGTTGCAGCACATT+TGG - Chr2:60432270-60432289 MsG0280009902.01.T01:intron 40.0%
! AAAAACCAGAAGCGTGAAGA+AGG + Chr2:60433626-60433645 None:intergenic 40.0%
! AGCCTCAACATCAGCATTTT+AGG + Chr2:60431930-60431949 None:intergenic 40.0%
! CTCTATCTTTGGTAGCATCT+TGG - Chr2:60433932-60433951 MsG0280009902.01.T01:intron 40.0%
! GAAAGGGTGTGTATCAAACT+CGG - Chr2:60432897-60432916 MsG0280009902.01.T01:intron 40.0%
! GACCTGTTCTGACAATTTTC+AGG - Chr2:60432041-60432060 MsG0280009902.01.T01:intron 40.0%
! GCTACGAAGTATCAGGAAAA+GGG - Chr2:60432856-60432875 MsG0280009902.01.T01:intron 40.0%
! TATCTCCGTTTTCTCTCAAC+AGG - Chr2:60433413-60433432 MsG0280009902.01.T01:intron 40.0%
! TCTCAGCAGATTTTTACGGT+AGG + Chr2:60432770-60432789 None:intergenic 40.0%
! TGACAAAATATGGTGCTGGA+AGG - Chr2:60432598-60432617 MsG0280009902.01.T01:intron 40.0%
! TGTAGTCTTTGTTTGGTTGC+AGG - Chr2:60432343-60432362 MsG0280009902.01.T01:intron 40.0%
!! AGCACAAGGTACTGTTGTTA+GGG + Chr2:60433898-60433917 None:intergenic 40.0%
!! ATTGGGTTGACTACTGGTAT+TGG + Chr2:60430961-60430980 None:intergenic 40.0%
!! CAAATCGAAATGGTGCCATA+TGG - Chr2:60432630-60432649 MsG0280009902.01.T01:intron 40.0%
!! GTCAACCCAATGGGTTATAT+AGG - Chr2:60430970-60430989 MsG0280009902.01.T01:three_prime_UTR 40.0%
!! TACCTTGTGCTGTTTCAAGT+TGG - Chr2:60433907-60433926 MsG0280009902.01.T01:intron 40.0%
!! TCAAGTTGGTCCTCTATCTT+TGG - Chr2:60433921-60433940 MsG0280009902.01.T01:intron 40.0%
!!! CACGCTTCTGGTTTTTTCTT+CGG - Chr2:60433630-60433649 MsG0280009902.01.T01:intron 40.0%
!!! GGTTGTGCTTTTTCCTGTAT+TGG - Chr2:60433434-60433453 MsG0280009902.01.T01:intron 40.0%
AGCTGGTAAATATGTTGCCG+TGG - Chr2:60433028-60433047 MsG0280009902.01.T01:intron 45.0%
ATGTTCTTGCCAGGAGTCTT+GGG - Chr2:60433655-60433674 MsG0280009902.01.T01:intron 45.0%
ATTCAGTCCTTCTTCTCAGG+AGG + Chr2:60433980-60433999 None:intergenic 45.0%
CATGCCAACTTCAAGGGATA+TGG + Chr2:60433703-60433722 None:intergenic 45.0%
CTTCCATCACATTATGCAGC+TGG - Chr2:60432105-60432124 MsG0280009902.01.T01:intron 45.0%
GACCAACTTGAAACAGCACA+AGG + Chr2:60433912-60433931 None:intergenic 45.0%
GAGAATATGCAAGGCAAGCT+AGG + Chr2:60433259-60433278 None:intergenic 45.0%
GCTAGCAGCTGTCATTACAA+CGG + Chr2:60432500-60432519 None:intergenic 45.0%
TAATAAGCCAGCTGCATCCA+CGG + Chr2:60433048-60433067 None:intergenic 45.0%
TACCTGCATGCCAACTTCAA+GGG + Chr2:60433709-60433728 None:intergenic 45.0%
TAGTAGAGTCAGGGAGCTTA+TGG - Chr2:60431072-60431091 MsG0280009902.01.T01:three_prime_UTR 45.0%
TATGTTCTTGCCAGGAGTCT+TGG - Chr2:60433654-60433673 MsG0280009902.01.T01:intron 45.0%
TCTAAGAATACCTGTCCACC+AGG + Chr2:60433596-60433615 None:intergenic 45.0%
TCTTCGGATATGTTCTTGCC+AGG - Chr2:60433646-60433665 MsG0280009902.01.T01:intron 45.0%
TCTTCTGCATCCTTATGTCC+TGG - Chr2:60433575-60433594 MsG0280009902.01.T01:intron 45.0%
TTACCTGCATGCCAACTTCA+AGG + Chr2:60433710-60433729 None:intergenic 45.0%
TTGGTTGCCTCACATTGTGT+TGG - Chr2:60432203-60432222 MsG0280009902.01.T01:intron 45.0%
TTTCTGACAGCCAAACTAGC+TGG - Chr2:60433011-60433030 MsG0280009902.01.T01:intron 45.0%
! ATATTAGTTGGGTGCTGTCC+TGG - Chr2:60432132-60432151 MsG0280009902.01.T01:intron 45.0%
! ATCTTTGGTAGCATCTTGGC+TGG - Chr2:60433936-60433955 MsG0280009902.01.T01:intron 45.0%
! GCTAGTTTGGCTGTCAGAAA+AGG + Chr2:60433011-60433030 None:intergenic 45.0%
! GGCTACGAAGTATCAGGAAA+AGG - Chr2:60432855-60432874 MsG0280009902.01.T01:intron 45.0%
! TAGGGGAATTCCAAAGTGCT+TGG + Chr2:60433880-60433899 None:intergenic 45.0%
! TATTAGTTGGGTGCTGTCCT+GGG - Chr2:60432133-60432152 MsG0280009902.01.T01:intron 45.0%
!! CAGCACAAGGTACTGTTGTT+AGG + Chr2:60433899-60433918 None:intergenic 45.0%
!! GCACAAGGTACTGTTGTTAG+GGG + Chr2:60433897-60433916 None:intergenic 45.0%
!! TAACCCATTGGGTTGACTAC+TGG + Chr2:60430967-60430986 None:intergenic 45.0%
!!! GTGCTTTTTCCTGTATTGGC+TGG - Chr2:60433438-60433457 MsG0280009902.01.T01:intron 45.0%
ACAGCTAAGGGTGGCATCAA+TGG + Chr2:60433510-60433529 None:intergenic 50.0%
AGACTAAAACTCGTACCCCC+AGG + Chr2:60432153-60432172 None:intergenic 50.0%
ATCCCTTGAAGTTGGCATGC+AGG - Chr2:60433704-60433723 MsG0280009902.01.T01:intron 50.0%
CCACAGTTGGCTACAGCTAA+GGG + Chr2:60433522-60433541 None:intergenic 50.0%
CGGACCATATCCCTTGAAGT+TGG - Chr2:60433696-60433715 MsG0280009902.01.T01:intron 50.0%
GACAGCTGCTAGCACTCTTT+CGG - Chr2:60432507-60432526 MsG0280009902.01.T01:CDS 50.0%
GTAGCATCTTGGCTGGTATG+TGG - Chr2:60433943-60433962 MsG0280009902.01.T01:intron 50.0%
TACCTGTCCACCAGGACATA+AGG + Chr2:60433588-60433607 None:intergenic 50.0%
TCAGTTCTCGGCGTAGTTTC+TGG - Chr2:60433851-60433870 MsG0280009902.01.T01:intron 50.0%
TCTGCATCCTTATGTCCTGG+TGG - Chr2:60433578-60433597 MsG0280009902.01.T01:intron 50.0%
! AGAAGTGCACCAGCCAATAC+AGG + Chr2:60433450-60433469 None:intergenic 50.0%
! ATCCTTATGTCCTGGTGGAC+AGG - Chr2:60433583-60433602 MsG0280009902.01.T01:intron 50.0%
! ATTAGTTGGGTGCTGTCCTG+GGG - Chr2:60432134-60432153 MsG0280009902.01.T01:intron 50.0%
!! TTTCTGGCTGCCAAGCACTT+TGG - Chr2:60433867-60433886 MsG0280009902.01.T01:intron 50.0%
AACATCCAGCCCAAGACTCC+TGG + Chr2:60433667-60433686 None:intergenic 55.0%
CAGTTGGCTACAGCTAAGGG+TGG + Chr2:60433519-60433538 None:intergenic 55.0%
CCCTTAGCTGTAGCCAACTG+TGG - Chr2:60433519-60433538 MsG0280009902.01.T01:intron 55.0%
GATACACACCCTTTCCCCCA+AGG + Chr2:60432892-60432911 None:intergenic 55.0%
GCCACAGTTGGCTACAGCTA+AGG + Chr2:60433523-60433542 None:intergenic 55.0%
GCTCTGTCCTCCTGAGAAGA+AGG - Chr2:60433970-60433989 MsG0280009902.01.T01:intron 55.0%
GGCTGGATGTTGCATCATCG+CGG - Chr2:60433676-60433695 MsG0280009902.01.T01:intron 55.0%
GTCTGCCTTCTTCACGCTTC+TGG - Chr2:60433618-60433637 MsG0280009902.01.T01:intron 55.0%
TATGTTGCCGTGGATGCAGC+TGG - Chr2:60433038-60433057 MsG0280009902.01.T01:intron 55.0%
TGCTAGCACTCTTTCGGCTG+TGG - Chr2:60432513-60432532 MsG0280009902.01.T01:CDS 55.0%
! TTAGTTGGGTGCTGTCCTGG+GGG - Chr2:60432135-60432154 MsG0280009902.01.T01:intron 55.0%
TCTTGCCAGGAGTCTTGGGC+TGG - Chr2:60433659-60433678 MsG0280009902.01.T01:intron 60.0%
TGCGATTGTGGTGCTCGCAG+AGG - Chr2:60431903-60431922 MsG0280009902.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 60430934 60434095 60430934 ID=MsG0280009902.01;Name=MsG0280009902.01
Chr2 mRNA 60430934 60434095 60430934 ID=MsG0280009902.01.T01;Parent=MsG0280009902.01;Name=MsG0280009902.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0.5|0.8|0.8|0|0|5|359|105
Chr2 exon 60430934 60431181 60430934 ID=MsG0280009902.01.T01:exon:6192;Parent=MsG0280009902.01.T01
Chr2 exon 60431304 60431594 60431304 ID=MsG0280009902.01.T01:exon:6191;Parent=MsG0280009902.01.T01
Chr2 exon 60432495 60432549 60432495 ID=MsG0280009902.01.T01:exon:6190;Parent=MsG0280009902.01.T01
Chr2 exon 60432631 60432664 60432631 ID=MsG0280009902.01.T01:exon:6189;Parent=MsG0280009902.01.T01
Chr2 exon 60434047 60434095 60434047 ID=MsG0280009902.01.T01:exon:6188;Parent=MsG0280009902.01.T01
Chr2 CDS 60434047 60434095 60434047 ID=MsG0280009902.01.T01:cds;Parent=MsG0280009902.01.T01
Chr2 CDS 60432631 60432664 60432631 ID=MsG0280009902.01.T01:cds;Parent=MsG0280009902.01.T01
Chr2 CDS 60432495 60432549 60432495 ID=MsG0280009902.01.T01:cds;Parent=MsG0280009902.01.T01
Chr2 CDS 60431415 60431594 60431415 ID=MsG0280009902.01.T01:cds;Parent=MsG0280009902.01.T01
Chr2 three_prime_UTR 60431304 60431414 60431304 ID=MsG0280009902.01.T01:three_prime_utr;Parent=MsG0280009902.01.T01
Chr2 three_prime_UTR 60430934 60431181 60430934 ID=MsG0280009902.01.T01:three_prime_utr;Parent=MsG0280009902.01.T01
Gene Sequence

>MsG0280009902.01.T01

ATGAGAACAATTCAAGGTCTCAAGCCAATACCAGTAGTCAACCCAATGGGCACAGCTAGTAATATTGTAACATATCTTGCACGTGGAAACGTGGCGCTTTCCGTTGTAATGACAGCTGCTAGCACTCTTTCGGCTGTGGTTGTGCTTTTTCCTGTATTGGCTGGTGCACTTCTGAATCAGTATTTCCAACCTCTTGTTAAACTTGTGTCTCCATTGATGCCACCCTTAGCTGTAGCCAACTGTGGCAATTTTAATGTGGAATTGCAATTGCACAGAGCTCTTCTGCATCCTTATGTCCTGGTGGACAGGTATTCTTAG
Protein sequence

>MsG0280009902.01.T01

MRTIQGLKPIPVVNPMGTASNIVTYLARGNVALSVVMTAASTLSAVVVLFPVLAGALLNQYFQPLVKLVSPLMPPLAVANCGNFNVELQLHRALLHPYVLVDRYS*