AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009939.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009939.01.T01 MTR_2g073370 98.980 294 3 0 1 294 1 294 0.0 612
MsG0280009939.01.T01 MTR_4g067320 60.550 109 43 0 1 109 1 109 5.69e-43 147
MsG0280009939.01.T01 MTR_4g067320 60.550 109 43 0 1 109 1 109 5.78e-43 147
MsG0280009939.01.T01 MTR_2g099010 40.104 192 80 3 1 192 1 157 2.45e-42 146
MsG0280009939.01.T01 MTR_2g099010 40.104 192 80 3 1 192 1 157 2.77e-42 145
MsG0280009939.01.T01 MTR_3g117320 50.685 146 71 1 1 145 1 146 6.82e-41 142
MsG0280009939.01.T01 MTR_4g071200 54.717 106 47 1 1 105 1 106 9.01e-36 128
MsG0280009939.01.T01 MTR_3g113070 54.717 106 46 1 1 104 1 106 1.06e-35 128
MsG0280009939.01.T01 MTR_1g023260 54.286 105 47 1 1 104 1 105 1.43e-35 129
MsG0280009939.01.T01 MTR_1g023260 54.286 105 47 1 1 104 1 105 3.81e-35 125
MsG0280009939.01.T01 MTR_5g021580 50.000 104 49 2 1 104 1 101 1.55e-26 103
MsG0280009939.01.T01 MTR_5g021580 50.000 104 49 2 1 104 1 101 1.77e-26 103
MsG0280009939.01.T01 MTR_1g013450 41.667 96 47 1 5 100 6 92 1.02e-18 85.1
MsG0280009939.01.T01 MTR_3g105710 44.792 96 44 1 5 100 6 92 1.64e-18 84.0
MsG0280009939.01.T01 MTR_7g018170 43.478 92 43 1 5 96 26 108 1.83e-17 82.0
MsG0280009939.01.T01 MTR_4g128930 38.043 92 48 1 5 96 22 104 1.73e-14 73.6
MsG0280009939.01.T01 MTR_5g069480 33.333 96 55 1 1 96 1 87 3.01e-11 63.9
MsG0280009939.01.T01 MTR_5g069480 33.333 96 55 1 1 96 1 87 5.00e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009939.01.T01 AT1G78600 52.797 286 113 4 1 273 1 277 8.17e-94 280
MsG0280009939.01.T01 AT1G78600 48.693 306 115 5 1 273 1 297 1.79e-91 274
MsG0280009939.01.T01 AT1G06040 64.220 109 39 0 1 109 1 109 1.83e-46 154
MsG0280009939.01.T01 AT1G06040 64.220 109 39 0 1 109 1 109 7.05e-46 155
MsG0280009939.01.T01 AT2G31380 61.468 109 42 0 1 109 1 109 2.42e-42 146
MsG0280009939.01.T01 AT4G10240 49.701 167 62 5 1 161 1 151 8.73e-41 139
MsG0280009939.01.T01 AT1G75540 40.090 222 114 6 1 211 1 214 5.14e-36 132
MsG0280009939.01.T01 AT4G39070 49.640 139 68 2 1 138 1 138 1.94e-35 128
MsG0280009939.01.T01 AT2G21320 50.000 104 49 2 1 104 1 101 1.67e-26 102
MsG0280009939.01.T01 AT4G38960 40.580 138 78 3 1 138 1 134 3.24e-26 102
MsG0280009939.01.T01 AT4G38960 40.580 138 78 3 1 138 1 134 3.24e-26 102
MsG0280009939.01.T01 AT5G15850 42.857 98 47 1 5 102 12 100 3.13e-18 84.0
MsG0280009939.01.T01 AT2G24790 41.667 96 47 1 5 100 8 94 9.87e-18 82.0
MsG0280009939.01.T01 AT5G24930 42.708 96 46 1 5 100 6 92 1.03e-17 82.8
MsG0280009939.01.T01 AT4G38960 30.939 181 78 4 1 138 1 177 1.29e-17 80.5
MsG0280009939.01.T01 AT3G02380 43.011 93 44 1 5 97 16 99 2.00e-17 81.6
MsG0280009939.01.T01 AT2G24790 41.667 96 47 1 5 100 8 94 2.93e-17 79.3
MsG0280009939.01.T01 AT5G15840 37.755 98 52 1 5 102 20 108 5.42e-16 77.8
MsG0280009939.01.T01 AT5G15840 39.785 93 47 1 5 97 20 103 1.15e-15 75.9
MsG0280009939.01.T01 AT2G47890 38.710 93 48 1 5 97 13 96 1.08e-12 67.8
MsG0280009939.01.T01 AT2G47890 38.710 93 48 1 5 97 13 96 1.14e-12 67.4
MsG0280009939.01.T01 AT2G47890 38.710 93 48 1 5 97 13 96 3.24e-12 65.5
MsG0280009939.01.T01 AT5G57660 34.783 92 47 2 5 96 22 100 3.09e-11 63.5
MsG0280009939.01.T01 AT4G38960 33.981 103 64 3 36 138 3 101 6.13e-11 60.1
MsG0280009939.01.T01 AT4G38960 33.981 103 64 3 36 138 3 101 6.13e-11 60.1

Find 81 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCGGAATCATCATTAATT+TGG 0.177392 2:+61332130 MsG0280009939.01.T01:exon
TTGCTTGAAAATTTGAAATT+TGG 0.187546 2:+61329467 MsG0280009939.01.T01:five_prime_UTR
AATTTCTAACTCCACTTAAT+TGG 0.204807 2:+61332299 MsG0280009939.01.T01:three_prime_UTR
TCTTCATTGGATCTTAAAAT+AGG 0.258790 2:-61331911 None:intergenic
CCTACGGAAGCCGGAGGATT+TGG 0.274830 2:+61331016 MsG0280009939.01.T01:CDS
CCACCAGCCAAGGTGTCTTA+TGG 0.275750 2:+61331046 MsG0280009939.01.T01:CDS
TAGTTTAATATTTCTATTCA+TGG 0.285262 2:+61332251 MsG0280009939.01.T01:three_prime_UTR
GAGCATTGTCTATAAGTTAT+AGG 0.293532 2:-61332169 None:intergenic
GTATTCCATTTCCTCTTCAT+TGG 0.304492 2:-61331924 None:intergenic
AATTCATTGAAGACAGATTC+TGG 0.306694 2:+61330908 MsG0280009939.01.T01:CDS
CGATAATGCTCTATTTGTTC+CGG 0.320546 2:+61332054 MsG0280009939.01.T01:CDS
GAATTTATTCAATAGTATAT+TGG 0.322036 2:-61332536 None:intergenic
CTACAGCTTCGGGGCTAAAC+TGG 0.326015 2:+61332008 MsG0280009939.01.T01:CDS
TTCCGTGATCAGTAGCTTCT+AGG 0.348013 2:-61330877 None:intergenic
CCATCACCACCTACAGCTTC+GGG 0.354633 2:+61331998 MsG0280009939.01.T01:CDS
ACCTGACAAATATCACATTT+AGG 0.368052 2:-61329672 None:intergenic
GAGGTTAAAGATTCAAACTT+TGG 0.385924 2:+61329415 MsG0280009939.01.T01:five_prime_UTR
AGCATTGTCTATAAGTTATA+GGG 0.389360 2:-61332168 None:intergenic
ATCTGTCTTCAATGAATTTG+AGG 0.389947 2:-61330903 None:intergenic
CCCATCACCACCTACAGCTT+CGG 0.391074 2:+61331997 MsG0280009939.01.T01:CDS
AAGATCCAATGAAGAGGAAA+TGG 0.393956 2:+61331919 MsG0280009939.01.T01:CDS
GGAACTGTCCAAGACGAATC+TGG 0.395347 2:-61331968 None:intergenic
TGATGAAGCTGCTTTGTGTT+GGG 0.412693 2:+61329566 MsG0280009939.01.T01:CDS
ATCTTCTTCTGTCTCGAGAA+AGG 0.428233 2:+61330952 MsG0280009939.01.T01:CDS
GCTCTATTTGTTCCGGACAT+AGG 0.432303 2:+61332061 MsG0280009939.01.T01:CDS
TAAACCTTTCAGGAAGCATT+CGG 0.445968 2:+61330734 MsG0280009939.01.T01:intron
CCACTTAATTGGAGTGATGA+AGG 0.446186 2:+61332310 MsG0280009939.01.T01:three_prime_UTR
GCCTAAATGTGATATTTGTC+AGG 0.459129 2:+61329671 MsG0280009939.01.T01:CDS
CTGATGAAGCTGCTTTGTGT+TGG 0.463397 2:+61329565 MsG0280009939.01.T01:CDS
CCTTCATCACTCCAATTAAG+TGG 0.473774 2:-61332310 None:intergenic
CCCGAAGCTGTAGGTGGTGA+TGG 0.475557 2:-61331998 None:intergenic
TCTCAAGTTTCTAAAAGAGT+TGG 0.479388 2:+61332471 MsG0280009939.01.T01:three_prime_UTR
TATGGTGGTGGTTCTAACTC+TGG 0.487845 2:+61331064 MsG0280009939.01.T01:CDS
GAAATGTTGCCTACGGAAGC+CGG 0.488655 2:+61331007 MsG0280009939.01.T01:CDS
TGGATGGATCATCAAATTCA+AGG 0.498918 2:+61331155 MsG0280009939.01.T01:CDS
CAGTCAGAATTATAACTACA+TGG 0.503150 2:+61331135 MsG0280009939.01.T01:CDS
AAAGATTCAAACTTTGGTGT+GGG 0.503932 2:+61329421 MsG0280009939.01.T01:five_prime_UTR
GCCAAGGTGTCTTATGGTGG+TGG 0.508324 2:+61331052 MsG0280009939.01.T01:CDS
CCACCATAAGACACCTTGGC+TGG 0.515474 2:-61331049 None:intergenic
ACTGCAAATGCATTTGTCTC+AGG 0.516070 2:+61330818 MsG0280009939.01.T01:CDS
TGGAAAATCTCCAAATCCTC+CGG 0.517904 2:-61331026 None:intergenic
CAGAATTATAACTACATGGA+TGG 0.518101 2:+61331139 MsG0280009939.01.T01:CDS
TCAAATACATACATCTACAT+TGG 0.519092 2:-61332383 None:intergenic
GGGAAAATTGAAACTTGAAG+TGG 0.523387 2:+61329441 MsG0280009939.01.T01:five_prime_UTR
TAAAGATTCAAACTTTGGTG+TGG 0.527418 2:+61329420 MsG0280009939.01.T01:five_prime_UTR
ACCGTGTTCCAGATTCGTCT+TGG 0.527866 2:+61331960 MsG0280009939.01.T01:CDS
GGAAAATTGAAACTTGAAGT+GGG 0.530897 2:+61329442 MsG0280009939.01.T01:five_prime_UTR
TCATTGTACTCAGATGATTG+AGG 0.549321 2:-61330986 None:intergenic
TGAGAAGAAGAAGCAGAAAG+AGG 0.549965 2:-61329645 None:intergenic
CTTCTCACCGGTGTAAAAGT+CGG 0.551046 2:+61330854 MsG0280009939.01.T01:CDS
CCGAAGCTGTAGGTGGTGAT+GGG 0.554827 2:-61331997 None:intergenic
AATGGAATACGACGACTACA+TGG 0.559471 2:+61331937 MsG0280009939.01.T01:CDS
CAGTTTAGCCCCGAAGCTGT+AGG 0.563024 2:-61332007 None:intergenic
ATGCTAGTAAAGCCTATGTC+CGG 0.569585 2:-61332073 None:intergenic
CATCAAAGGTTTCTTCTCAC+CGG 0.573699 2:+61330842 MsG0280009939.01.T01:CDS
CCAGCCAAGGTGTCTTATGG+TGG 0.576805 2:+61331049 MsG0280009939.01.T01:CDS
GGATTCTAGAAATGAAATGA+AGG 0.581456 2:-61332362 None:intergenic
CATTTGTCTCAGGACATCAA+AGG 0.584028 2:+61330828 MsG0280009939.01.T01:CDS
CCAAATCCTCCGGCTTCCGT+AGG 0.585959 2:-61331016 None:intergenic
ACCACCACCATAAGACACCT+TGG 0.592536 2:-61331053 None:intergenic
TTGTGAAGGTGCTGAAGCTA+AGG 0.593143 2:+61329530 MsG0280009939.01.T01:CDS
CCATAAGACACCTTGGCTGG+TGG 0.594137 2:-61331046 None:intergenic
GGCCTAGAAGCTACTGATCA+CGG 0.598771 2:+61330875 MsG0280009939.01.T01:CDS
GGATGGATCATCAAATTCAA+GGG 0.601507 2:+61331156 MsG0280009939.01.T01:CDS
TGTCTATAAGTTATAGGGAA+GGG 0.601577 2:-61332163 None:intergenic
GTACAATGAAATGTTGCCTA+CGG 0.602602 2:+61331000 MsG0280009939.01.T01:CDS
CATTATCGAATGAATACCGA+GGG 0.607602 2:-61332039 None:intergenic
CTTGATGTGTAAGAAAACAA+TGG 0.609904 2:+61332507 MsG0280009939.01.T01:three_prime_UTR
TTGTCTATAAGTTATAGGGA+AGG 0.610254 2:-61332164 None:intergenic
AAACTTGAGATGTATAGTGA+AGG 0.624114 2:-61332458 None:intergenic
ATTCAGTGTAATGTTTGTGA+AGG 0.627076 2:+61329516 MsG0280009939.01.T01:CDS
CCAAATTAATGATGATTCCG+CGG 0.629743 2:-61332130 None:intergenic
AGTTATAGGGAAGGGATCAG+AGG 0.634893 2:-61332155 None:intergenic
TTTAGCCCCGAAGCTGTAGG+TGG 0.663191 2:-61332004 None:intergenic
TCCAAGACGAATCTGGAACA+CGG 0.671574 2:-61331961 None:intergenic
GTAGGTGGTGATGGGATCTG+AGG 0.674067 2:-61331989 None:intergenic
CATCACCACCTACAGCTTCG+GGG 0.682711 2:+61331999 MsG0280009939.01.T01:CDS
GAGTTTATGCAGGCTGACAG+TGG 0.687400 2:+61331866 MsG0280009939.01.T01:intron
GCATTATCGAATGAATACCG+AGG 0.703310 2:-61332040 None:intergenic
ATGTTGCCTACGGAAGCCGG+AGG 0.732134 2:+61331010 MsG0280009939.01.T01:CDS
ACTCTGGAAACATGTCACAG+TGG 0.763112 2:+61331080 MsG0280009939.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTCAAAAATATAAATAAA+AGG - Chr2:61329968-61329987 None:intergenic 10.0%
!! ATGAAAAAGTAAAAAAAAAT+TGG + Chr2:61329821-61329840 MsG0280009939.01.T01:intron 10.0%
!!! ATTTTTGTGATTTTTTTATT+TGG + Chr2:61329712-61329731 MsG0280009939.01.T01:intron 10.0%
!!! TTTAATTGTAATATTTTGAT+GGG + Chr2:61329987-61330006 MsG0280009939.01.T01:intron 10.0%
!!! TTTTTATTGATTTATTTGAA+TGG + Chr2:61329761-61329780 MsG0280009939.01.T01:intron 10.0%
!! CAAATAGAAAAAAAAATTGA+GGG - Chr2:61331206-61331225 None:intergenic 15.0%
!! GAATTTATTCAATAGTATAT+TGG - Chr2:61332539-61332558 None:intergenic 15.0%
!! TCATAAAAACTTATCATTTA+AGG + Chr2:61330235-61330254 MsG0280009939.01.T01:intron 15.0%
!!! CTTTAATTGTAATATTTTGA+TGG + Chr2:61329986-61330005 MsG0280009939.01.T01:intron 15.0%
!!! GAAATCTATAATTTTGTATA+GGG + Chr2:61330106-61330125 MsG0280009939.01.T01:intron 15.0%
!!! TAGTTTAATATTTCTATTCA+TGG + Chr2:61332251-61332270 MsG0280009939.01.T01:three_prime_UTR 15.0%
!!! TGAAATCTATAATTTTGTAT+AGG + Chr2:61330105-61330124 MsG0280009939.01.T01:intron 15.0%
!!! TGATTTTTTTATTTGGTATA+TGG + Chr2:61329719-61329738 MsG0280009939.01.T01:intron 15.0%
!! AAAAATTACTACTATTCTGA+GGG - Chr2:61332105-61332124 None:intergenic 20.0%
!! CCAAATAGAAAAAAAAATTG+AGG - Chr2:61331207-61331226 None:intergenic 20.0%
!! TTAAAAAGTTTATCCAAACA+AGG - Chr2:61331566-61331585 None:intergenic 20.0%
!! TTAGAAACAGGTAAAATATT+TGG + Chr2:61330061-61330080 MsG0280009939.01.T01:intron 20.0%
!! TTAGTTCCAGAATTATATAA+AGG - Chr2:61330023-61330042 None:intergenic 20.0%
!! TTGAAACCTTTATATAATTC+TGG + Chr2:61330014-61330033 MsG0280009939.01.T01:intron 20.0%
!!! AATAAGTTTAAAACAGCTTA+TGG + Chr2:61331697-61331716 MsG0280009939.01.T01:intron 20.0%
!!! ATAATTTTTCTCAACTTTTG+AGG + Chr2:61329396-61329415 MsG0280009939.01.T01:five_prime_UTR 20.0%
!!! CCTCAATTTTTTTTTCTATT+TGG + Chr2:61331204-61331223 MsG0280009939.01.T01:intron 20.0%
!!! TTGCTTGAAAATTTGAAATT+TGG + Chr2:61329467-61329486 MsG0280009939.01.T01:five_prime_UTR 20.0%
!!! TTTTAAGTGCTTTTTAACTA+TGG + Chr2:61331424-61331443 MsG0280009939.01.T01:intron 20.0%
!!! TTTTGAAAATTAATTTGGAG+TGG + Chr2:61329790-61329809 MsG0280009939.01.T01:intron 20.0%
!!! TTTTTTTTCTATTTGGATCA+TGG + Chr2:61331211-61331230 MsG0280009939.01.T01:intron 20.0%
! AATGCAGAAATATTAAGAAC+TGG + Chr2:61331346-61331365 MsG0280009939.01.T01:intron 25.0%
! AATTTCTAACTCCACTTAAT+TGG + Chr2:61332299-61332318 MsG0280009939.01.T01:three_prime_UTR 25.0%
! AGCATTGTCTATAAGTTATA+GGG - Chr2:61332171-61332190 None:intergenic 25.0%
! ATGAATGTTGAAAGTAGTAA+AGG + Chr2:61331510-61331529 MsG0280009939.01.T01:intron 25.0%
! CTGGTAATAATTTGAGAAAA+TGG + Chr2:61330466-61330485 MsG0280009939.01.T01:intron 25.0%
! GAAAAATTACTACTATTCTG+AGG - Chr2:61332106-61332125 None:intergenic 25.0%
! GGACAAATAACAAATTAACA+AGG - Chr2:61330680-61330699 None:intergenic 25.0%
! TCAAATACATACATCTACAT+TGG - Chr2:61332386-61332405 None:intergenic 25.0%
! TCTTCATTGGATCTTAAAAT+AGG - Chr2:61331914-61331933 None:intergenic 25.0%
!! ACTTAAATTTTCTCTGTAAG+AGG - Chr2:61332227-61332246 None:intergenic 25.0%
!! TGGACAATTGATGAATTTTT+CGG + Chr2:61331100-61331119 MsG0280009939.01.T01:CDS 25.0%
!! TTTTGGTCTCTAATATTTGT+TGG + Chr2:61330305-61330324 MsG0280009939.01.T01:intron 25.0%
!!! TATTTTAAGATCCAATGAAG+AGG + Chr2:61331913-61331932 MsG0280009939.01.T01:CDS 25.0%
!!! TGTTAATTTGTTATTTGTCC+TGG + Chr2:61330680-61330699 MsG0280009939.01.T01:intron 25.0%
!!! TTGTGTTATTTAAACCTTTC+AGG + Chr2:61330724-61330743 MsG0280009939.01.T01:intron 25.0%
!!! TTTTATTTGGTATATGGTTG+TGG + Chr2:61329725-61329744 MsG0280009939.01.T01:intron 25.0%
AAACTTGAGATGTATAGTGA+AGG - Chr2:61332461-61332480 None:intergenic 30.0%
AAATCCTTTCCCTTGAATAT+AGG + Chr2:61330391-61330410 MsG0280009939.01.T01:intron 30.0%
AATTCATTGAAGACAGATTC+TGG + Chr2:61330908-61330927 MsG0280009939.01.T01:CDS 30.0%
ACAACTACATGTGTTAATGT+CGG + Chr2:61330501-61330520 MsG0280009939.01.T01:intron 30.0%
ACCTGACAAATATCACATTT+AGG - Chr2:61329675-61329694 None:intergenic 30.0%
ATCTGTCTTCAATGAATTTG+AGG - Chr2:61330906-61330925 None:intergenic 30.0%
ATTCAGTGTAATGTTTGTGA+AGG + Chr2:61329516-61329535 MsG0280009939.01.T01:CDS 30.0%
CAAGATACTCCTATATTCAA+GGG - Chr2:61330403-61330422 None:intergenic 30.0%
CAGAATTATAACTACATGGA+TGG + Chr2:61331139-61331158 MsG0280009939.01.T01:CDS 30.0%
CAGTCAGAATTATAACTACA+TGG + Chr2:61331135-61331154 MsG0280009939.01.T01:CDS 30.0%
CTCAACAAAAATGTTGACAA+TGG - Chr2:61330651-61330670 None:intergenic 30.0%
CTGCATTTACAATGTACATT+AGG - Chr2:61331333-61331352 None:intergenic 30.0%
CTTGATGTGTAAGAAAACAA+TGG + Chr2:61332507-61332526 MsG0280009939.01.T01:three_prime_UTR 30.0%
GAGGTTAAAGATTCAAACTT+TGG + Chr2:61329415-61329434 MsG0280009939.01.T01:five_prime_UTR 30.0%
GGAAAATTGAAACTTGAAGT+GGG + Chr2:61329442-61329461 MsG0280009939.01.T01:five_prime_UTR 30.0%
GTTCTTGATAGATTAGAAAC+AGG + Chr2:61330049-61330068 MsG0280009939.01.T01:intron 30.0%
TAAAGATTCAAACTTTGGTG+TGG + Chr2:61329420-61329439 MsG0280009939.01.T01:five_prime_UTR 30.0%
TAAGGTCCTTAAATATTGTC+CGG + Chr2:61330253-61330272 MsG0280009939.01.T01:intron 30.0%
TCAAATAAGCCAATTCAAAC+AGG + Chr2:61331789-61331808 MsG0280009939.01.T01:intron 30.0%
TGTCTATAAGTTATAGGGAA+GGG - Chr2:61332166-61332185 None:intergenic 30.0%
TGTGAACATTGTCATATGAT+TGG + Chr2:61331286-61331305 MsG0280009939.01.T01:intron 30.0%
TTCTATGACAAGTTAGAAAG+TGG + Chr2:61330356-61330375 MsG0280009939.01.T01:intron 30.0%
TTGTCTATAAGTTATAGGGA+AGG - Chr2:61332167-61332186 None:intergenic 30.0%
TTTATGGTTGAATGCTTATG+CGG + Chr2:61331833-61331852 MsG0280009939.01.T01:intron 30.0%
! AAAGATTCAAACTTTGGTGT+GGG + Chr2:61329421-61329440 MsG0280009939.01.T01:five_prime_UTR 30.0%
! CATTGTCAACATTTTTGTTG+AGG + Chr2:61330649-61330668 MsG0280009939.01.T01:intron 30.0%
! GACAGAAGAAGATTTTGTAT+CGG - Chr2:61330945-61330964 None:intergenic 30.0%
! GAGCATTGTCTATAAGTTAT+AGG - Chr2:61332172-61332191 None:intergenic 30.0%
! GGATTCTAGAAATGAAATGA+AGG - Chr2:61332365-61332384 None:intergenic 30.0%
! TCTCAAGTTTCTAAAAGAGT+TGG + Chr2:61332471-61332490 MsG0280009939.01.T01:three_prime_UTR 30.0%
! TTTTCTATCTTCTCTGTTCA+TGG + Chr2:61331257-61331276 MsG0280009939.01.T01:intron 30.0%
!! AAGCTAATAGTTGTTTTCAC+AGG + Chr2:61331653-61331672 MsG0280009939.01.T01:intron 30.0%
!!! GGTTGTTTTAGTTTCTTCTT+TGG + Chr2:61332272-61332291 MsG0280009939.01.T01:three_prime_UTR 30.0%
AAGATCCAATGAAGAGGAAA+TGG + Chr2:61331919-61331938 MsG0280009939.01.T01:CDS 35.0%
AAGGTCCTTAAATATTGTCC+GGG + Chr2:61330254-61330273 MsG0280009939.01.T01:intron 35.0%
AAGTTCTTTCAAACTGAACC+AGG - Chr2:61330701-61330720 None:intergenic 35.0%
AGTAGCAGTATATCCTTGTT+TGG + Chr2:61331550-61331569 MsG0280009939.01.T01:intron 35.0%
AGTGCTATATAGGTTTCTTG+TGG + Chr2:61330560-61330579 MsG0280009939.01.T01:intron 35.0%
ATTGGATCACTGACAAAGAT+TGG + Chr2:61331304-61331323 MsG0280009939.01.T01:intron 35.0%
CATTATCGAATGAATACCGA+GGG - Chr2:61332042-61332061 None:intergenic 35.0%
CCAAATTAATGATGATTCCG+CGG - Chr2:61332133-61332152 None:intergenic 35.0%
CCAAGATACTCCTATATTCA+AGG - Chr2:61330404-61330423 None:intergenic 35.0%
CGATAATGCTCTATTTGTTC+CGG + Chr2:61332054-61332073 MsG0280009939.01.T01:CDS 35.0%
GAACCAACAAAAACTGAACA+AGG - Chr2:61332422-61332441 None:intergenic 35.0%
GCCTAAATGTGATATTTGTC+AGG + Chr2:61329671-61329690 MsG0280009939.01.T01:CDS 35.0%
GGATGGATCATCAAATTCAA+GGG + Chr2:61331156-61331175 MsG0280009939.01.T01:CDS 35.0%
GGGAAAATTGAAACTTGAAG+TGG + Chr2:61329441-61329460 MsG0280009939.01.T01:five_prime_UTR 35.0%
GTACAATGAAATGTTGCCTA+CGG + Chr2:61331000-61331019 MsG0280009939.01.T01:CDS 35.0%
GTATTCCATTTCCTCTTCAT+TGG - Chr2:61331927-61331946 None:intergenic 35.0%
TACTCCTATATTCAAGGGAA+AGG - Chr2:61330398-61330417 None:intergenic 35.0%
TCATTGTACTCAGATGATTG+AGG - Chr2:61330989-61331008 None:intergenic 35.0%
TGGATGGATCATCAAATTCA+AGG + Chr2:61331155-61331174 MsG0280009939.01.T01:CDS 35.0%
! AATGAATACCGAGGGTTTTT+CGG - Chr2:61332034-61332053 None:intergenic 35.0%
! AGTGTTTGAGGCACTATTTA+AGG + Chr2:61330140-61330159 MsG0280009939.01.T01:intron 35.0%
! CTAAAGTGTCAGTGCTATAT+AGG + Chr2:61330550-61330569 MsG0280009939.01.T01:intron 35.0%
! GATCCTTGTTCAGTTTTTGT+TGG + Chr2:61332416-61332435 MsG0280009939.01.T01:three_prime_UTR 35.0%
! TCTAACTTGTCATAGAAGCA+AGG - Chr2:61330353-61330372 None:intergenic 35.0%
! TGGTCTCTAATATTTGTTGG+TGG + Chr2:61330308-61330327 MsG0280009939.01.T01:intron 35.0%
!! CCTTGAATATAGGAGTATCT+TGG + Chr2:61330401-61330420 MsG0280009939.01.T01:intron 35.0%
!! TAAACCTTTCAGGAAGCATT+CGG + Chr2:61330734-61330753 MsG0280009939.01.T01:intron 35.0%
!! TGAATATAGGAGTATCTTGG+TGG + Chr2:61330404-61330423 MsG0280009939.01.T01:intron 35.0%
!!! GTAGTAATTTTTCAAGACCG+CGG + Chr2:61332113-61332132 MsG0280009939.01.T01:CDS 35.0%
AATCGCTTAACACTGCAGTA+AGG - Chr2:61331815-61331834 None:intergenic 40.0%
AATGGAATACGACGACTACA+TGG + Chr2:61331937-61331956 MsG0280009939.01.T01:CDS 40.0%
ACAGAAATCAGACCCAACAT+TGG + Chr2:61330433-61330452 MsG0280009939.01.T01:intron 40.0%
ACTGCAAATGCATTTGTCTC+AGG + Chr2:61330818-61330837 MsG0280009939.01.T01:CDS 40.0%
AGGTCCTTAAATATTGTCCG+GGG + Chr2:61330255-61330274 MsG0280009939.01.T01:intron 40.0%
AGTGTCAACGTTCCAATGTT+GGG - Chr2:61330448-61330467 None:intergenic 40.0%
ATCTTCTTCTGTCTCGAGAA+AGG + Chr2:61330952-61330971 MsG0280009939.01.T01:CDS 40.0%
ATGCTAGTAAAGCCTATGTC+CGG - Chr2:61332076-61332095 None:intergenic 40.0%
CATCAAAGGTTTCTTCTCAC+CGG + Chr2:61330842-61330861 MsG0280009939.01.T01:CDS 40.0%
CATTTGTCTCAGGACATCAA+AGG + Chr2:61330828-61330847 MsG0280009939.01.T01:CDS 40.0%
CCACTTAATTGGAGTGATGA+AGG + Chr2:61332310-61332329 MsG0280009939.01.T01:three_prime_UTR 40.0%
CCGCGGAATCATCATTAATT+TGG + Chr2:61332130-61332149 MsG0280009939.01.T01:exon 40.0%
CCTTCATCACTCCAATTAAG+TGG - Chr2:61332313-61332332 None:intergenic 40.0%
CTGCAGTGTTAAGCGATTTA+TGG + Chr2:61331817-61331836 MsG0280009939.01.T01:intron 40.0%
GCATTATCGAATGAATACCG+AGG - Chr2:61332043-61332062 None:intergenic 40.0%
TCCTTCTGATGAGTTTATGC+AGG + Chr2:61331856-61331875 MsG0280009939.01.T01:intron 40.0%
TGAGAAGAAGAAGCAGAAAG+AGG - Chr2:61329648-61329667 None:intergenic 40.0%
TGGAAAATCTCCAAATCCTC+CGG - Chr2:61331029-61331048 None:intergenic 40.0%
! ATATGGTTGTGGACTATGAG+AGG + Chr2:61329736-61329755 MsG0280009939.01.T01:intron 40.0%
! TGATGAAGCTGCTTTGTGTT+GGG + Chr2:61329566-61329585 MsG0280009939.01.T01:CDS 40.0%
!!! CGGCTATTTCTTTTGTCTAG+AGG + Chr2:61330754-61330773 MsG0280009939.01.T01:CDS 40.0%
AGTTATAGGGAAGGGATCAG+AGG - Chr2:61332158-61332177 None:intergenic 45.0%
ATAGCCGAATGCTTCCTGAA+AGG - Chr2:61330741-61330760 None:intergenic 45.0%
CAACATTGGAACGTTGACAC+TGG + Chr2:61330447-61330466 MsG0280009939.01.T01:intron 45.0%
CAGTGTCAACGTTCCAATGT+TGG - Chr2:61330449-61330468 None:intergenic 45.0%
CTTCTCACCGGTGTAAAAGT+CGG + Chr2:61330854-61330873 MsG0280009939.01.T01:CDS 45.0%
GCAGTAAGGCCTGTTTGAAT+TGG - Chr2:61331801-61331820 None:intergenic 45.0%
GCCTGCATAAACTCATCAGA+AGG - Chr2:61331860-61331879 None:intergenic 45.0%
GCTCTATTTGTTCCGGACAT+AGG + Chr2:61332061-61332080 MsG0280009939.01.T01:CDS 45.0%
GGTCCTTAAATATTGTCCGG+GGG + Chr2:61330256-61330275 MsG0280009939.01.T01:intron 45.0%
GTCTTGCTAACGAGTGTTTG+AGG + Chr2:61330128-61330147 MsG0280009939.01.T01:intron 45.0%
TAAACTGGCCGAAAAACCCT+CGG + Chr2:61332023-61332042 MsG0280009939.01.T01:CDS 45.0%
TTCCGTGATCAGTAGCTTCT+AGG - Chr2:61330880-61330899 None:intergenic 45.0%
! ACTCTGGAAACATGTCACAG+TGG + Chr2:61331080-61331099 MsG0280009939.01.T01:CDS 45.0%
! CTGATGAAGCTGCTTTGTGT+TGG + Chr2:61329565-61329584 MsG0280009939.01.T01:CDS 45.0%
! GCTCGATAGCATTTCGCTTT+TGG + Chr2:61330288-61330307 MsG0280009939.01.T01:intron 45.0%
! TCCAAGACGAATCTGGAACA+CGG - Chr2:61331964-61331983 None:intergenic 45.0%
!! TATGGTGGTGGTTCTAACTC+TGG + Chr2:61331064-61331083 MsG0280009939.01.T01:CDS 45.0%
!! TTCTAGGCCGACTTTTACAC+CGG - Chr2:61330864-61330883 None:intergenic 45.0%
!! TTGTGAAGGTGCTGAAGCTA+AGG + Chr2:61329530-61329549 MsG0280009939.01.T01:CDS 45.0%
!!! AATTATGAATTTTTTTTTTT+TGG - Chr2:61329382-61329401 None:intergenic 5.0%
!!! ATTATGAATTTTTTTTTTTT+GGG - Chr2:61329381-61329400 None:intergenic 5.0%
!!! ATTTTTTTTGAAAATTAATT+TGG + Chr2:61329785-61329804 MsG0280009939.01.T01:intron 5.0%
ACCACCACCATAAGACACCT+TGG - Chr2:61331056-61331075 None:intergenic 50.0%
ACCGTGTTCCAGATTCGTCT+TGG + Chr2:61331960-61331979 MsG0280009939.01.T01:CDS 50.0%
GAAATGTTGCCTACGGAAGC+CGG + Chr2:61331007-61331026 MsG0280009939.01.T01:CDS 50.0%
GAGTTTATGCAGGCTGACAG+TGG + Chr2:61331866-61331885 MsG0280009939.01.T01:intron 50.0%
GGAACTGTCCAAGACGAATC+TGG - Chr2:61331971-61331990 None:intergenic 50.0%
GGCCTAGAAGCTACTGATCA+CGG + Chr2:61330875-61330894 MsG0280009939.01.T01:CDS 50.0%
! TGGAGATTTTCCACCAGCCA+AGG + Chr2:61331036-61331055 MsG0280009939.01.T01:CDS 50.0%
CAGTTTAGCCCCGAAGCTGT+AGG - Chr2:61332010-61332029 None:intergenic 55.0%
CATCACCACCTACAGCTTCG+GGG + Chr2:61331999-61332018 MsG0280009939.01.T01:CDS 55.0%
CCACCATAAGACACCTTGGC+TGG - Chr2:61331052-61331071 None:intergenic 55.0%
CCATAAGACACCTTGGCTGG+TGG - Chr2:61331049-61331068 None:intergenic 55.0%
CCATCACCACCTACAGCTTC+GGG + Chr2:61331998-61332017 MsG0280009939.01.T01:CDS 55.0%
CCCATCACCACCTACAGCTT+CGG + Chr2:61331997-61332016 MsG0280009939.01.T01:CDS 55.0%
CCGAAGCTGTAGGTGGTGAT+GGG - Chr2:61332000-61332019 None:intergenic 55.0%
CTACAGCTTCGGGGCTAAAC+TGG + Chr2:61332008-61332027 MsG0280009939.01.T01:CDS 55.0%
GCGCCCCCGGACAATATTTA+AGG - Chr2:61330262-61330281 None:intergenic 55.0%
TTTAGCCCCGAAGCTGTAGG+TGG - Chr2:61332007-61332026 None:intergenic 55.0%
! CCACCAGCCAAGGTGTCTTA+TGG + Chr2:61331046-61331065 MsG0280009939.01.T01:CDS 55.0%
! CCAGCCAAGGTGTCTTATGG+TGG + Chr2:61331049-61331068 MsG0280009939.01.T01:CDS 55.0%
! GCCAAGGTGTCTTATGGTGG+TGG + Chr2:61331052-61331071 MsG0280009939.01.T01:CDS 55.0%
!! GTAGGTGGTGATGGGATCTG+AGG - Chr2:61331992-61332011 None:intergenic 55.0%
ATGTTGCCTACGGAAGCCGG+AGG + Chr2:61331010-61331029 MsG0280009939.01.T01:CDS 60.0%
CCAAATCCTCCGGCTTCCGT+AGG - Chr2:61331019-61331038 None:intergenic 60.0%
CCCGAAGCTGTAGGTGGTGA+TGG - Chr2:61332001-61332020 None:intergenic 60.0%
CCTACGGAAGCCGGAGGATT+TGG + Chr2:61331016-61331035 MsG0280009939.01.T01:CDS 60.0%
ATCGAGCTATCGAGCGCCCC+CGG - Chr2:61330275-61330294 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 61329373 61332580 61329373 ID=MsG0280009939.01;Name=MsG0280009939.01
Chr2 mRNA 61329373 61332580 61329373 ID=MsG0280009939.01.T01;Parent=MsG0280009939.01;Name=MsG0280009939.01.T01;_AED=0.29;_eAED=0.29;_QI=137|1|1|1|1|1|3|433|294
Chr2 exon 61329373 61329692 61329373 ID=MsG0280009939.01.T01:exon:34467;Parent=MsG0280009939.01.T01
Chr2 exon 61330746 61331177 61330746 ID=MsG0280009939.01.T01:exon:34468;Parent=MsG0280009939.01.T01
Chr2 exon 61331878 61332580 61331878 ID=MsG0280009939.01.T01:exon:34469;Parent=MsG0280009939.01.T01
Chr2 five_prime_UTR 61329373 61329509 61329373 ID=MsG0280009939.01.T01:five_prime_utr;Parent=MsG0280009939.01.T01
Chr2 CDS 61329510 61329692 61329510 ID=MsG0280009939.01.T01:cds;Parent=MsG0280009939.01.T01
Chr2 CDS 61330746 61331177 61330746 ID=MsG0280009939.01.T01:cds;Parent=MsG0280009939.01.T01
Chr2 CDS 61331878 61332147 61331878 ID=MsG0280009939.01.T01:cds;Parent=MsG0280009939.01.T01
Chr2 three_prime_UTR 61332148 61332580 61332148 ID=MsG0280009939.01.T01:three_prime_utr;Parent=MsG0280009939.01.T01
Gene Sequence

>MsG0280009939.01.T01

ATGAAGATTCAGTGTAATGTTTGTGAAGGTGCTGAAGCTAAGGTTCTTTGTTGTGCTGATGAAGCTGCTTTGTGTTGGGAATGTGATGAGAAAGTTCATGCTGCTAATAAACTTGCTAGCAAACATCAAAGAGTTCCTCTTTCTGCTTCTTCTTCTCATATGCCTAAATGTGATATTTGTCAGGAAGCATTCGGCTATTTCTTTTGTCTAGAGGATCGCGCTTTACTCTGCAGAAAATGTGATTTAGCAATACACACTGCAAATGCATTTGTCTCAGGACATCAAAGGTTTCTTCTCACCGGTGTAAAAGTCGGCCTAGAAGCTACTGATCACGGAACTTCCTCAAATTCATTGAAGACAGATTCTGGTGAGAAAGTTTCCGATACAAAATCTTCTTCTGTCTCGAGAAAGGTTTCGCAAATGCCTCAATCATCTGAGTACAATGAAATGTTGCCTACGGAAGCCGGAGGATTTGGAGATTTTCCACCAGCCAAGGTGTCTTATGGTGGTGGTTCTAACTCTGGAAACATGTCACAGTGGACAATTGATGAATTTTTCGGCGTAAATGATTTCAGTCAGAATTATAACTACATGGATGGATCATCAAATTCAAGGGCTGACAGTGGTAAGCTTGAAGATTCTGATTCTCCTATTTTAAGATCCAATGAAGAGGAAATGGAATACGACGACTACATGGACCGTGTTCCAGATTCGTCTTGGACAGTTCCTCAGATCCCATCACCACCTACAGCTTCGGGGCTAAACTGGCCGAAAAACCCTCGGTATTCATTCGATAATGCTCTATTTGTTCCGGACATAGGCTTTACTAGCATGCAATACCCTCAGAATAGTAGTAATTTTTCAAGACCGCGGAATCATCATTAA
Protein sequence

>MsG0280009939.01.T01

MKIQCNVCEGAEAKVLCCADEAALCWECDEKVHAANKLASKHQRVPLSASSSHMPKCDICQEAFGYFFCLEDRALLCRKCDLAIHTANAFVSGHQRFLLTGVKVGLEATDHGTSSNSLKTDSGEKVSDTKSSSVSRKVSQMPQSSEYNEMLPTEAGGFGDFPPAKVSYGGGSNSGNMSQWTIDEFFGVNDFSQNYNYMDGSSNSRADSGKLEDSDSPILRSNEEEMEYDDYMDRVPDSSWTVPQIPSPPTASGLNWPKNPRYSFDNALFVPDIGFTSMQYPQNSSNFSRPRNHH*