Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034380.01.T01 | XP_003619730.1 | 92.982 | 342 | 20 | 1 | 1 | 338 | 1 | 342 | 0 | 662 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034380.01.T01 | Q9LT10 | 54.07 | 344 | 136 | 5 | 2 | 337 | 3 | 332 | 8.09E-140 | 402 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034380.01.T01 | G7KNW4 | 92.982 | 342 | 20 | 1 | 1 | 338 | 1 | 342 | 0.0 | 662 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014669.01 | MsG0680034380.01 | 0.808695 | 2.728835e-50 | 1.570734e-47 |
| MsG0680034380.01 | MsG0880046472.01 | 0.802191 | 6.307221e-49 | 3.070088e-46 |
| MsG0480021250.01 | MsG0680034380.01 | 0.833695 | 4.601555e-56 | 5.339628e-53 |
| MsG0480023945.01 | MsG0680034380.01 | 0.862634 | 4.410535e-64 | 1.300380e-60 |
| MsG0380016053.01 | MsG0680034380.01 | 0.809756 | 1.616648e-50 | 9.572760e-48 |
| MsG0280009939.01 | MsG0680034380.01 | 0.824966 | 6.059502e-54 | 5.449778e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0680034380.01 | MsG0780040621.01 | PPI |
| MsG0180004920.01 | MsG0680034380.01 | PPI |
| MsG0180004920.01 | MsG0680034380.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034380.01.T01 | MTR_6g065270 | 92.982 | 342 | 20 | 1 | 1 | 338 | 1 | 342 | 0.0 | 662 |
| MsG0680034380.01.T01 | MTR_6g065370 | 81.081 | 333 | 63 | 0 | 6 | 338 | 8 | 340 | 0.0 | 577 |
| MsG0680034380.01.T01 | MTR_6g065400 | 79.580 | 333 | 68 | 0 | 6 | 338 | 8 | 340 | 0.0 | 569 |
| MsG0680034380.01.T01 | MTR_6g065120 | 77.246 | 334 | 75 | 1 | 6 | 338 | 9 | 342 | 0.0 | 555 |
| MsG0680034380.01.T01 | MTR_6g065340 | 81.682 | 333 | 45 | 1 | 6 | 338 | 19 | 335 | 0.0 | 542 |
| MsG0680034380.01.T01 | MTR_6g065110 | 68.923 | 325 | 101 | 0 | 8 | 332 | 11 | 335 | 1.29e-177 | 496 |
| MsG0680034380.01.T01 | MTR_6g065430 | 57.658 | 333 | 132 | 2 | 3 | 333 | 4 | 329 | 2.21e-142 | 406 |
| MsG0680034380.01.T01 | MTR_1g082210 | 37.202 | 336 | 202 | 3 | 2 | 332 | 10 | 341 | 2.53e-74 | 233 |
| MsG0680034380.01.T01 | MTR_7g093950 | 37.059 | 340 | 205 | 4 | 1 | 332 | 9 | 347 | 5.98e-74 | 232 |
| MsG0680034380.01.T01 | MTR_1g089310 | 37.092 | 337 | 203 | 3 | 1 | 332 | 9 | 341 | 1.44e-72 | 228 |
| MsG0680034380.01.T01 | MTR_1g089310 | 37.092 | 337 | 203 | 3 | 1 | 332 | 25 | 357 | 2.14e-72 | 229 |
| MsG0680034380.01.T01 | MTR_6g464280 | 36.496 | 274 | 160 | 5 | 30 | 296 | 24 | 290 | 1.25e-50 | 171 |
| MsG0680034380.01.T01 | MTR_6g464510 | 36.957 | 276 | 156 | 9 | 29 | 296 | 27 | 292 | 2.77e-47 | 162 |
| MsG0680034380.01.T01 | MTR_8g035540 | 33.121 | 314 | 189 | 6 | 29 | 331 | 13 | 316 | 5.01e-46 | 159 |
| MsG0680034380.01.T01 | MTR_7g406880 | 32.907 | 313 | 181 | 9 | 44 | 333 | 45 | 351 | 1.44e-45 | 159 |
| MsG0680034380.01.T01 | MTR_8g035500 | 32.177 | 317 | 198 | 6 | 23 | 331 | 10 | 317 | 1.51e-45 | 158 |
| MsG0680034380.01.T01 | MTR_4g050140 | 31.210 | 314 | 194 | 5 | 29 | 333 | 17 | 317 | 5.08e-45 | 156 |
| MsG0680034380.01.T01 | MTR_6g464320 | 34.043 | 282 | 172 | 5 | 22 | 296 | 17 | 291 | 1.07e-43 | 153 |
| MsG0680034380.01.T01 | MTR_8g035520 | 32.890 | 301 | 180 | 6 | 47 | 336 | 40 | 329 | 5.03e-43 | 151 |
| MsG0680034380.01.T01 | MTR_0007s0520 | 38.249 | 217 | 119 | 4 | 44 | 254 | 37 | 244 | 1.79e-42 | 150 |
| MsG0680034380.01.T01 | MTR_1g012430 | 32.903 | 310 | 188 | 8 | 29 | 333 | 15 | 309 | 6.04e-42 | 148 |
| MsG0680034380.01.T01 | MTR_1g102160 | 36.900 | 271 | 144 | 10 | 29 | 285 | 23 | 280 | 1.19e-41 | 148 |
| MsG0680034380.01.T01 | MTR_1g111280 | 33.003 | 303 | 172 | 11 | 45 | 331 | 51 | 338 | 1.70e-40 | 145 |
| MsG0680034380.01.T01 | MTR_7g107040 | 31.973 | 294 | 179 | 5 | 53 | 336 | 39 | 321 | 2.95e-40 | 144 |
| MsG0680034380.01.T01 | MTR_3g012440 | 30.723 | 332 | 195 | 6 | 29 | 333 | 19 | 342 | 6.80e-40 | 144 |
| MsG0680034380.01.T01 | MTR_1g102170 | 32.906 | 234 | 131 | 5 | 30 | 254 | 43 | 259 | 2.31e-39 | 142 |
| MsG0680034380.01.T01 | MTR_3g101420 | 32.558 | 301 | 171 | 9 | 47 | 333 | 34 | 316 | 5.08e-38 | 138 |
| MsG0680034380.01.T01 | MTR_6g464120 | 34.052 | 232 | 132 | 5 | 30 | 254 | 29 | 246 | 6.51e-37 | 139 |
| MsG0680034380.01.T01 | MTR_4g086540 | 30.921 | 304 | 176 | 7 | 36 | 334 | 33 | 307 | 6.89e-37 | 135 |
| MsG0680034380.01.T01 | MTR_4g086510 | 31.186 | 295 | 183 | 7 | 47 | 332 | 35 | 318 | 2.39e-35 | 131 |
| MsG0680034380.01.T01 | MTR_4g086480 | 31.525 | 295 | 177 | 9 | 47 | 331 | 39 | 318 | 2.33e-34 | 128 |
| MsG0680034380.01.T01 | MTR_7g056207 | 30.667 | 300 | 186 | 8 | 47 | 336 | 67 | 354 | 4.04e-33 | 126 |
| MsG0680034380.01.T01 | MTR_4g086470 | 30.640 | 297 | 181 | 9 | 47 | 333 | 38 | 319 | 5.55e-30 | 117 |
| MsG0680034380.01.T01 | MTR_1g105020 | 29.054 | 296 | 182 | 8 | 51 | 331 | 40 | 322 | 1.27e-29 | 115 |
| MsG0680034380.01.T01 | MTR_6g022710 | 29.818 | 275 | 147 | 5 | 89 | 322 | 156 | 425 | 2.56e-29 | 117 |
| MsG0680034380.01.T01 | MTR_3g450680 | 28.179 | 291 | 181 | 7 | 51 | 326 | 48 | 325 | 7.01e-27 | 108 |
| MsG0680034380.01.T01 | MTR_5g005900 | 28.659 | 328 | 191 | 10 | 29 | 333 | 21 | 328 | 2.29e-26 | 107 |
| MsG0680034380.01.T01 | MTR_5g005920 | 27.692 | 325 | 198 | 12 | 29 | 333 | 25 | 332 | 5.00e-26 | 106 |
| MsG0680034380.01.T01 | MTR_5g005850 | 28.523 | 298 | 182 | 8 | 51 | 331 | 45 | 328 | 2.67e-25 | 104 |
| MsG0680034380.01.T01 | MTR_8g040860 | 27.740 | 292 | 178 | 10 | 51 | 326 | 48 | 322 | 2.00e-24 | 102 |
| MsG0680034380.01.T01 | MTR_7g051020 | 30.625 | 320 | 191 | 9 | 29 | 333 | 24 | 327 | 1.57e-23 | 99.4 |
| MsG0680034380.01.T01 | MTR_6g065470 | 33.529 | 170 | 81 | 3 | 6 | 157 | 7 | 162 | 1.07e-21 | 90.5 |
| MsG0680034380.01.T01 | MTR_1g104930 | 29.467 | 319 | 193 | 11 | 29 | 333 | 24 | 324 | 5.64e-19 | 86.7 |
| MsG0680034380.01.T01 | MTR_1g104933 | 28.621 | 290 | 177 | 8 | 57 | 333 | 46 | 318 | 2.10e-17 | 82.0 |
| MsG0680034380.01.T01 | MTR_6g065480 | 53.448 | 58 | 26 | 1 | 231 | 288 | 1 | 57 | 2.80e-15 | 69.7 |
| MsG0680034380.01.T01 | MTR_0007s0480 | 38.462 | 91 | 50 | 2 | 169 | 254 | 1 | 90 | 5.37e-14 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034380.01.T01 | AT5G23530 | 54.070 | 344 | 136 | 5 | 2 | 337 | 3 | 332 | 8.24e-141 | 402 |
| MsG0680034380.01.T01 | AT5G27320 | 37.910 | 335 | 202 | 2 | 1 | 332 | 9 | 340 | 4.26e-78 | 243 |
| MsG0680034380.01.T01 | AT3G63010 | 35.942 | 345 | 209 | 5 | 1 | 338 | 9 | 348 | 1.77e-70 | 224 |
| MsG0680034380.01.T01 | AT3G05120 | 35.015 | 337 | 211 | 2 | 1 | 332 | 9 | 342 | 2.41e-69 | 220 |
| MsG0680034380.01.T01 | AT5G62180 | 34.824 | 313 | 189 | 10 | 30 | 334 | 21 | 326 | 1.97e-51 | 173 |
| MsG0680034380.01.T01 | AT5G06570 | 35.965 | 342 | 184 | 9 | 9 | 335 | 8 | 329 | 4.85e-45 | 157 |
| MsG0680034380.01.T01 | AT5G06570 | 35.965 | 342 | 184 | 9 | 9 | 335 | 8 | 329 | 4.85e-45 | 157 |
| MsG0680034380.01.T01 | AT2G45600 | 32.881 | 295 | 174 | 6 | 56 | 337 | 38 | 321 | 6.12e-45 | 156 |
| MsG0680034380.01.T01 | AT5G06570 | 35.965 | 342 | 184 | 9 | 9 | 335 | 23 | 344 | 6.57e-45 | 157 |
| MsG0680034380.01.T01 | AT5G06570 | 35.965 | 342 | 184 | 9 | 9 | 335 | 23 | 344 | 6.57e-45 | 157 |
| MsG0680034380.01.T01 | AT5G16080 | 33.808 | 281 | 160 | 8 | 46 | 314 | 54 | 320 | 2.91e-44 | 155 |
| MsG0680034380.01.T01 | AT1G68620 | 31.707 | 287 | 175 | 7 | 54 | 332 | 57 | 330 | 1.12e-42 | 151 |
| MsG0680034380.01.T01 | AT2G45610 | 33.929 | 280 | 168 | 6 | 30 | 300 | 22 | 293 | 1.40e-42 | 150 |
| MsG0680034380.01.T01 | AT1G47480 | 30.816 | 331 | 203 | 10 | 11 | 336 | 5 | 314 | 4.52e-41 | 146 |
| MsG0680034380.01.T01 | AT1G19190 | 31.861 | 317 | 184 | 13 | 30 | 333 | 18 | 315 | 3.94e-33 | 125 |
| MsG0680034380.01.T01 | AT3G27320 | 27.632 | 380 | 182 | 9 | 23 | 322 | 37 | 403 | 2.32e-31 | 122 |
| MsG0680034380.01.T01 | AT3G48700 | 29.773 | 309 | 180 | 12 | 47 | 332 | 32 | 326 | 3.88e-31 | 120 |
| MsG0680034380.01.T01 | AT5G14310 | 31.408 | 277 | 143 | 6 | 90 | 322 | 148 | 421 | 2.72e-30 | 120 |
| MsG0680034380.01.T01 | AT2G03550 | 31.879 | 298 | 175 | 9 | 47 | 333 | 32 | 312 | 5.15e-30 | 117 |
| MsG0680034380.01.T01 | AT3G27320 | 30.225 | 311 | 165 | 8 | 54 | 322 | 135 | 435 | 1.28e-29 | 118 |
| MsG0680034380.01.T01 | AT3G48690 | 28.904 | 301 | 188 | 9 | 47 | 332 | 32 | 321 | 1.30e-28 | 113 |
| MsG0680034380.01.T01 | AT1G49640 | 31.330 | 233 | 147 | 8 | 50 | 279 | 36 | 258 | 5.04e-27 | 108 |
| MsG0680034380.01.T01 | AT1G49660 | 28.522 | 291 | 186 | 9 | 51 | 331 | 36 | 314 | 1.15e-25 | 105 |
| MsG0680034380.01.T01 | AT1G49650 | 29.961 | 257 | 161 | 8 | 51 | 299 | 92 | 337 | 8.24e-22 | 95.5 |
Find 104 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACAGTTCCGGTGCAAATT+TGG | 0.242565 | 6:+86620151 | MsG0680034380.01.T01:CDS |
| GACAGGAAGGGAGGTAGTTT+TGG | 0.265065 | 6:-86619886 | None:intergenic |
| TGCGGAGGATTTGTCGGGTT+TGG | 0.280062 | 6:+86620400 | MsG0680034380.01.T01:CDS |
| GGCACATCATCTTGCAGTTC+GGG | 0.281818 | 6:+86620172 | MsG0680034380.01.T01:CDS |
| GGCGTTAACGGTAATGTCTT+TGG | 0.307568 | 6:-86619793 | None:intergenic |
| CTCTTTACTCCCACTGTTAC+CGG | 0.313439 | 6:+86619837 | MsG0680034380.01.T01:CDS |
| GCAGTTCGGGTTTGTAAAGC+TGG | 0.329901 | 6:+86620185 | MsG0680034380.01.T01:CDS |
| GGCAAAAGAGGTATTATGAT+TGG | 0.376841 | 6:+86620474 | MsG0680034380.01.T01:CDS |
| GTGGTGGAAGGTGTTCTTAC+CGG | 0.381206 | 6:+86620331 | MsG0680034380.01.T01:CDS |
| CTCCGGGAAATCCGGATTAT+CGG | 0.399962 | 6:+86620209 | MsG0680034380.01.T01:CDS |
| GGGTTTGTAAAGCTGGGCTC+CGG | 0.403578 | 6:+86620192 | MsG0680034380.01.T01:CDS |
| TTCCTTGCCGGAGACAGTTC+CGG | 0.404209 | 6:+86620140 | MsG0680034380.01.T01:CDS |
| ATCATTTACTTCCACGGCGG+CGG | 0.407976 | 6:+86619909 | MsG0680034380.01.T01:CDS |
| CCTAATGCTACTCCCGTTAA+CGG | 0.408492 | 6:+86619762 | MsG0680034380.01.T01:CDS |
| GATTTGATCCTTTGAATGAT+TGG | 0.409071 | 6:+86620453 | MsG0680034380.01.T01:CDS |
| GCCGAATGCGGAGGATTTGT+CGG | 0.409294 | 6:+86620394 | MsG0680034380.01.T01:CDS |
| ACTCAACACCTCTTCTTCCT+TGG | 0.411552 | 6:+86619631 | MsG0680034380.01.T01:CDS |
| TGGCACATCATCTTGCAGTT+CGG | 0.418774 | 6:+86620171 | MsG0680034380.01.T01:CDS |
| ACCCGATAATCCGGATTTCC+CGG | 0.425872 | 6:-86620211 | None:intergenic |
| TTTGGTGGAAACGCCGTTAA+CGG | 0.430126 | 6:-86619775 | None:intergenic |
| TCCGGGAAATCCGGATTATC+GGG | 0.432121 | 6:+86620210 | MsG0680034380.01.T01:CDS |
| TGAGTAGATTCAGGCAGATC+AGG | 0.435184 | 6:-86620566 | None:intergenic |
| AAGCTGGGCTCCGGGAAATC+CGG | 0.437572 | 6:+86620201 | MsG0680034380.01.T01:CDS |
| CCGAATGCGGAGGATTTGTC+GGG | 0.438724 | 6:+86620395 | MsG0680034380.01.T01:CDS |
| TAAAAGTGAAAGAAGTGAAA+TGG | 0.440270 | 6:-86619667 | None:intergenic |
| CCGTTAACGGGAGTAGCATT+AGG | 0.443886 | 6:-86619762 | None:intergenic |
| CCCGACAAATCCTCCGCATT+CGG | 0.445071 | 6:-86620395 | None:intergenic |
| TGGATACCAACCCGATAATC+CGG | 0.449037 | 6:-86620220 | None:intergenic |
| ACCGTCTCCGGTTACTTCTC+CGG | 0.451255 | 6:-86619856 | None:intergenic |
| GTGTCAAAATGTTTCCTTGC+CGG | 0.452264 | 6:+86620128 | MsG0680034380.01.T01:CDS |
| TTGGTGGAAACGCCGTTAAC+GGG | 0.454826 | 6:-86619774 | None:intergenic |
| CGCCGTTGGTGTCGATGGTA+AGG | 0.455201 | 6:+86620300 | MsG0680034380.01.T01:CDS |
| GATATCGATGCTCCGGTGTA+AGG | 0.456817 | 6:-86620028 | None:intergenic |
| TATGATTGGTTAAAGAAATG+TGG | 0.458980 | 6:+86620488 | MsG0680034380.01.T01:CDS |
| TTTGGTTGCACCACCGTCTC+CGG | 0.462872 | 6:-86619868 | None:intergenic |
| CTTTATGCCGTAGACTCTGC+AGG | 0.467753 | 6:+86619970 | MsG0680034380.01.T01:CDS |
| CATTCACCGCGTCATGATCT+CGG | 0.469524 | 6:-86620364 | None:intergenic |
| TTAACGCCGAAAATAACGTT+TGG | 0.477196 | 6:+86619808 | MsG0680034380.01.T01:CDS |
| GAGGCGGAGATTAAACTTGA+TGG | 0.482240 | 6:+86620275 | MsG0680034380.01.T01:CDS |
| ACTGTTACCGGAGAAGTAAC+CGG | 0.486574 | 6:+86619849 | MsG0680034380.01.T01:CDS |
| CTTTGAATGATTGGCAAAAG+AGG | 0.487397 | 6:+86620462 | MsG0680034380.01.T01:CDS |
| CCTGTCATCATTTACTTCCA+CGG | 0.488327 | 6:+86619903 | MsG0680034380.01.T01:CDS |
| ATGATTGGTTAAAGAAATGT+GGG | 0.488530 | 6:+86620489 | MsG0680034380.01.T01:CDS |
| TCATTTACTTCCACGGCGGC+GGG | 0.488815 | 6:+86619910 | MsG0680034380.01.T01:CDS |
| AAGAAGTGAAATGGTAGCTC+TGG | 0.489575 | 6:-86619658 | None:intergenic |
| ATTTGCACCGGAACTGTCTC+CGG | 0.491985 | 6:-86620147 | None:intergenic |
| CAGTTCGGGTTTGTAAAGCT+GGG | 0.506658 | 6:+86620186 | MsG0680034380.01.T01:CDS |
| GGTTTGTAAAGCTGGGCTCC+GGG | 0.520514 | 6:+86620193 | MsG0680034380.01.T01:CDS |
| CGATGGTAAGGACGGATTGG+TGG | 0.521258 | 6:+86620312 | MsG0680034380.01.T01:CDS |
| AAATTTCCCTGCAGAGTCTA+CGG | 0.521782 | 6:-86619977 | None:intergenic |
| ATAATCAACTGAGTAGATTC+AGG | 0.526163 | 6:-86620575 | None:intergenic |
| TGGTAAGGACGGATTGGTGG+TGG | 0.530541 | 6:+86620315 | MsG0680034380.01.T01:CDS |
| TGGATCGCCGTTGGTGTCGA+TGG | 0.540273 | 6:+86620295 | MsG0680034380.01.T01:CDS |
| TAAAGCATCGTGGTAGATTG+AGG | 0.544373 | 6:-86619952 | None:intergenic |
| GTTAAAGAGACGGCGGTTGA+CGG | 0.545760 | 6:-86619718 | None:intergenic |
| ATCGTGGTAGATTGAGGAAG+GGG | 0.546376 | 6:-86619946 | None:intergenic |
| TGATCTCGGTTCGATCCCTC+CGG | 0.547999 | 6:-86620350 | None:intergenic |
| AAGGACGGATTGGTGGTGGA+AGG | 0.550023 | 6:+86620319 | MsG0680034380.01.T01:CDS |
| TGCGACGGATATCGATGCTC+CGG | 0.555954 | 6:-86620035 | None:intergenic |
| CCGTGGAAGTAAATGATGAC+AGG | 0.560772 | 6:-86619903 | None:intergenic |
| ATCAAGTTTAATCTCCGCCT+CGG | 0.562819 | 6:-86620273 | None:intergenic |
| GTAAATGATGACAGGAAGGG+AGG | 0.564697 | 6:-86619895 | None:intergenic |
| CTGGCTTTCCAAGGAAGAAG+AGG | 0.565695 | 6:-86619639 | None:intergenic |
| GCATCGTGGTAGATTGAGGA+AGG | 0.566906 | 6:-86619948 | None:intergenic |
| GGCGCTTCTTCTCGCTCTAA+CGG | 0.569447 | 6:+86619693 | MsG0680034380.01.T01:CDS |
| TATCCGTCGCAATATGATGA+TGG | 0.571005 | 6:+86620047 | MsG0680034380.01.T01:CDS |
| TGTCGATGGTAAGGACGGAT+TGG | 0.572977 | 6:+86620309 | MsG0680034380.01.T01:CDS |
| TAAACTTGATGGATCGCCGT+TGG | 0.573185 | 6:+86620286 | MsG0680034380.01.T01:CDS |
| GGAAATCCGGATTATCGGGT+TGG | 0.574486 | 6:+86620214 | MsG0680034380.01.T01:CDS |
| ATGACGCGGTGAATGTAAGC+GGG | 0.586945 | 6:+86620372 | MsG0680034380.01.T01:CDS |
| ATGGTAGCTCTGGCTTTCCA+AGG | 0.587238 | 6:-86619648 | None:intergenic |
| TGGCGGTGAGGAACGGACCG+AGG | 0.589279 | 6:+86620256 | MsG0680034380.01.T01:CDS |
| GGAAGTAAATGATGACAGGA+AGG | 0.590018 | 6:-86619899 | None:intergenic |
| GTTAACGGTAATGTCTTTGG+TGG | 0.590282 | 6:-86619790 | None:intergenic |
| CACCGGAACTGTCTCCGGCA+AGG | 0.591057 | 6:-86620142 | None:intergenic |
| GACTGAAGAAGTTAAAGAGA+CGG | 0.595629 | 6:-86619728 | None:intergenic |
| GTGGAAGGTGTTCTTACCGG+AGG | 0.596371 | 6:+86620334 | MsG0680034380.01.T01:CDS |
| TCAGTTGATTATGCAAGTCA+AGG | 0.601932 | 6:+86620586 | MsG0680034380.01.T01:CDS |
| ATCGATGCTCCGGTGTAAGG+CGG | 0.602490 | 6:-86620025 | None:intergenic |
| GGTTACTTCTCCGGTAACAG+TGG | 0.608592 | 6:-86619847 | None:intergenic |
| ACCGGAGAAGTAACCGGAGA+CGG | 0.608834 | 6:+86619855 | MsG0680034380.01.T01:CDS |
| TGTAAGCGGGCCGAATGCGG+AGG | 0.609279 | 6:+86620385 | MsG0680034380.01.T01:CDS |
| GAAAACTGAACCCGCCGCCG+TGG | 0.611082 | 6:-86619920 | None:intergenic |
| ATGATGTGCCAAATTTGCAC+CGG | 0.614788 | 6:-86620159 | None:intergenic |
| GAATGTAAGCGGGCCGAATG+CGG | 0.618920 | 6:+86620382 | MsG0680034380.01.T01:CDS |
| GTCCTTACCATCGACACCAA+CGG | 0.621799 | 6:-86620302 | None:intergenic |
| TGGAAGGTGTTCTTACCGGA+GGG | 0.627234 | 6:+86620335 | MsG0680034380.01.T01:CDS |
| GTTACTTCTCCGGTAACAGT+GGG | 0.628682 | 6:-86619846 | None:intergenic |
| GTTGGTGTCGATGGTAAGGA+CGG | 0.629245 | 6:+86620304 | MsG0680034380.01.T01:CDS |
| TGTCAACTACCGCCTTACAC+CGG | 0.636801 | 6:+86620016 | MsG0680034380.01.T01:CDS |
| CATCGTGGTAGATTGAGGAA+GGG | 0.643584 | 6:-86619947 | None:intergenic |
| GAAGTAAATGATGACAGGAA+GGG | 0.653090 | 6:-86619898 | None:intergenic |
| TCTCCATCATCATATTGCGA+CGG | 0.653123 | 6:-86620050 | None:intergenic |
| TTTATGCCGTAGACTCTGCA+GGG | 0.653194 | 6:+86619971 | MsG0680034380.01.T01:CDS |
| GTCGCAATATGATGATGGAG+AGG | 0.653832 | 6:+86620052 | MsG0680034380.01.T01:CDS |
| CATGACGCGGTGAATGTAAG+CGG | 0.656915 | 6:+86620371 | MsG0680034380.01.T01:CDS |
| GTTGATTGAATATCCAAACA+TGG | 0.659983 | 6:+86620523 | MsG0680034380.01.T01:CDS |
| CGGTGAGGAACGGACCGAGG+CGG | 0.670710 | 6:+86620259 | MsG0680034380.01.T01:CDS |
| AACGGGAGTAGCATTAGGTG+AGG | 0.672219 | 6:-86619757 | None:intergenic |
| TGAAGAAGTTAAAGAGACGG+CGG | 0.716719 | 6:-86619725 | None:intergenic |
| GGAGAAGTAACCGGAGACGG+TGG | 0.726469 | 6:+86619858 | MsG0680034380.01.T01:CDS |
| GTCATCATTTACTTCCACGG+CGG | 0.732442 | 6:+86619906 | MsG0680034380.01.T01:CDS |
| ATCGAACCGAGATCATGACG+CGG | 0.753530 | 6:+86620358 | MsG0680034380.01.T01:CDS |
| GTCTACGGCATAAAGCATCG+TGG | 0.766027 | 6:-86619962 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAAAACATGAACCATGTT+TGG | - | Chr6:86620539-86620558 | None:intergenic | 25.0% |
| ! | TAAAAGTGAAAGAAGTGAAA+TGG | - | Chr6:86619670-86619689 | None:intergenic | 25.0% |
| !! | ATGATTGGTTAAAGAAATGT+GGG | + | Chr6:86620489-86620508 | MsG0680034380.01.T01:CDS | 25.0% |
| !! | TATGATTGGTTAAAGAAATG+TGG | + | Chr6:86620488-86620507 | MsG0680034380.01.T01:CDS | 25.0% |
| !!! | ATTTTTGGAAGAGAATAGAA+AGG | + | Chr6:86620085-86620104 | MsG0680034380.01.T01:CDS | 25.0% |
| !!! | TCACTTCTTTCACTTTTATT+CGG | + | Chr6:86619672-86619691 | MsG0680034380.01.T01:CDS | 25.0% |
| ATAATCAACTGAGTAGATTC+AGG | - | Chr6:86620578-86620597 | None:intergenic | 30.0% | |
| GATTTGATCCTTTGAATGAT+TGG | + | Chr6:86620453-86620472 | MsG0680034380.01.T01:CDS | 30.0% | |
| GTTGATTGAATATCCAAACA+TGG | + | Chr6:86620523-86620542 | MsG0680034380.01.T01:CDS | 30.0% | |
| ! | AGAGAATAGAAAGGTTTTAC+CGG | + | Chr6:86620094-86620113 | MsG0680034380.01.T01:CDS | 30.0% |
| !! | ACGAAACCAAACGTTATTTT+CGG | - | Chr6:86619817-86619836 | None:intergenic | 30.0% |
| CCAATAAAAACAAGCGTATC+CGG | - | Chr6:86620431-86620450 | None:intergenic | 35.0% | |
| CTTTGAATGATTGGCAAAAG+AGG | + | Chr6:86620462-86620481 | MsG0680034380.01.T01:CDS | 35.0% | |
| GAAGTAAATGATGACAGGAA+GGG | - | Chr6:86619901-86619920 | None:intergenic | 35.0% | |
| GACTGAAGAAGTTAAAGAGA+CGG | - | Chr6:86619731-86619750 | None:intergenic | 35.0% | |
| TCAGTTGATTATGCAAGTCA+AGG | + | Chr6:86620586-86620605 | MsG0680034380.01.T01:CDS | 35.0% | |
| TTAACGCCGAAAATAACGTT+TGG | + | Chr6:86619808-86619827 | MsG0680034380.01.T01:CDS | 35.0% | |
| ! | CTCTTTTGCCAATCATTCAA+AGG | - | Chr6:86620464-86620483 | None:intergenic | 35.0% |
| ! | TTTGACACATCAGCATTTTC+CGG | - | Chr6:86620116-86620135 | None:intergenic | 35.0% |
| !! | AACGTTATTTTCGGCGTTAA+CGG | - | Chr6:86619808-86619827 | None:intergenic | 35.0% |
| !! | GGCAAAAGAGGTATTATGAT+TGG | + | Chr6:86620474-86620493 | MsG0680034380.01.T01:CDS | 35.0% |
| !!! | GATACGCTTGTTTTTATTGG+TGG | + | Chr6:86620431-86620450 | MsG0680034380.01.T01:CDS | 35.0% |
| AAATTTCCCTGCAGAGTCTA+CGG | - | Chr6:86619980-86619999 | None:intergenic | 40.0% | |
| AAGAAGTGAAATGGTAGCTC+TGG | - | Chr6:86619661-86619680 | None:intergenic | 40.0% | |
| ATCAAGTTTAATCTCCGCCT+CGG | - | Chr6:86620276-86620295 | None:intergenic | 40.0% | |
| ATGATGTGCCAAATTTGCAC+CGG | - | Chr6:86620162-86620181 | None:intergenic | 40.0% | |
| CCTGTCATCATTTACTTCCA+CGG | + | Chr6:86619903-86619922 | MsG0680034380.01.T01:CDS | 40.0% | |
| GGAAGTAAATGATGACAGGA+AGG | - | Chr6:86619902-86619921 | None:intergenic | 40.0% | |
| GTGTCAAAATGTTTCCTTGC+CGG | + | Chr6:86620128-86620147 | MsG0680034380.01.T01:CDS | 40.0% | |
| GTTAACGGTAATGTCTTTGG+TGG | - | Chr6:86619793-86619812 | None:intergenic | 40.0% | |
| TATCCGTCGCAATATGATGA+TGG | + | Chr6:86620047-86620066 | MsG0680034380.01.T01:CDS | 40.0% | |
| TCTCCATCATCATATTGCGA+CGG | - | Chr6:86620053-86620072 | None:intergenic | 40.0% | |
| TGAAGAAGTTAAAGAGACGG+CGG | - | Chr6:86619728-86619747 | None:intergenic | 40.0% | |
| ! | AGAGGCTGTGCTGAAATTTT+TGG | + | Chr6:86620070-86620089 | MsG0680034380.01.T01:CDS | 40.0% |
| ! | TTTGTCGGGTTTGGATTTTC+CGG | + | Chr6:86620409-86620428 | MsG0680034380.01.T01:CDS | 40.0% |
| !! | TAAAGCATCGTGGTAGATTG+AGG | - | Chr6:86619955-86619974 | None:intergenic | 40.0% |
| !!! | CCGGATACGCTTGTTTTTAT+TGG | + | Chr6:86620428-86620447 | MsG0680034380.01.T01:CDS | 40.0% |
| !!! | GTATCCATTCAGCCGTTTTT+TGG | + | Chr6:86620236-86620255 | MsG0680034380.01.T01:CDS | 40.0% |
| ACTCAACACCTCTTCTTCCT+TGG | + | Chr6:86619631-86619650 | MsG0680034380.01.T01:CDS | 45.0% | |
| ACTGTTACCGGAGAAGTAAC+CGG | + | Chr6:86619849-86619868 | MsG0680034380.01.T01:CDS | 45.0% | |
| AGACAGTTCCGGTGCAAATT+TGG | + | Chr6:86620151-86620170 | MsG0680034380.01.T01:CDS | 45.0% | |
| ATCGTGGTAGATTGAGGAAG+GGG | - | Chr6:86619949-86619968 | None:intergenic | 45.0% | |
| CATCGTGGTAGATTGAGGAA+GGG | - | Chr6:86619950-86619969 | None:intergenic | 45.0% | |
| CCGTGGAAGTAAATGATGAC+AGG | - | Chr6:86619906-86619925 | None:intergenic | 45.0% | |
| CCTAATGCTACTCCCGTTAA+CGG | + | Chr6:86619762-86619781 | MsG0680034380.01.T01:CDS | 45.0% | |
| CTCTTTACTCCCACTGTTAC+CGG | + | Chr6:86619837-86619856 | MsG0680034380.01.T01:CDS | 45.0% | |
| GAGGCGGAGATTAAACTTGA+TGG | + | Chr6:86620275-86620294 | MsG0680034380.01.T01:CDS | 45.0% | |
| GGCGTTAACGGTAATGTCTT+TGG | - | Chr6:86619796-86619815 | None:intergenic | 45.0% | |
| GTAAATGATGACAGGAAGGG+AGG | - | Chr6:86619898-86619917 | None:intergenic | 45.0% | |
| GTCATCATTTACTTCCACGG+CGG | + | Chr6:86619906-86619925 | MsG0680034380.01.T01:CDS | 45.0% | |
| GTTACTTCTCCGGTAACAGT+GGG | - | Chr6:86619849-86619868 | None:intergenic | 45.0% | |
| TGAGTAGATTCAGGCAGATC+AGG | - | Chr6:86620569-86620588 | None:intergenic | 45.0% | |
| TGGATACCAACCCGATAATC+CGG | - | Chr6:86620223-86620242 | None:intergenic | 45.0% | |
| TGGCACATCATCTTGCAGTT+CGG | + | Chr6:86620171-86620190 | MsG0680034380.01.T01:CDS | 45.0% | |
| TTTATGCCGTAGACTCTGCA+GGG | + | Chr6:86619971-86619990 | MsG0680034380.01.T01:CDS | 45.0% | |
| TTTGGTGGAAACGCCGTTAA+CGG | - | Chr6:86619778-86619797 | None:intergenic | 45.0% | |
| ! | CAGTTCGGGTTTGTAAAGCT+GGG | + | Chr6:86620186-86620205 | MsG0680034380.01.T01:CDS | 45.0% |
| ! | TAAACTTGATGGATCGCCGT+TGG | + | Chr6:86620286-86620305 | MsG0680034380.01.T01:CDS | 45.0% |
| !! | GTCGCAATATGATGATGGAG+AGG | + | Chr6:86620052-86620071 | MsG0680034380.01.T01:CDS | 45.0% |
| !!! | TCCATTCAGCCGTTTTTTGG+CGG | + | Chr6:86620239-86620258 | MsG0680034380.01.T01:CDS | 45.0% |
| AACGGGAGTAGCATTAGGTG+AGG | - | Chr6:86619760-86619779 | None:intergenic | 50.0% | |
| ACCCGATAATCCGGATTTCC+CGG | - | Chr6:86620214-86620233 | None:intergenic | 50.0% | |
| ACCGCCAAAAAACGGCTGAA+TGG | - | Chr6:86620243-86620262 | None:intergenic | 50.0% | |
| ATCATTTACTTCCACGGCGG+CGG | + | Chr6:86619909-86619928 | MsG0680034380.01.T01:CDS | 50.0% | |
| ATCGAACCGAGATCATGACG+CGG | + | Chr6:86620358-86620377 | MsG0680034380.01.T01:CDS | 50.0% | |
| ATGACGCGGTGAATGTAAGC+GGG | + | Chr6:86620372-86620391 | MsG0680034380.01.T01:CDS | 50.0% | |
| ATTTGCACCGGAACTGTCTC+CGG | - | Chr6:86620150-86620169 | None:intergenic | 50.0% | |
| CATGACGCGGTGAATGTAAG+CGG | + | Chr6:86620371-86620390 | MsG0680034380.01.T01:CDS | 50.0% | |
| CATTCACCGCGTCATGATCT+CGG | - | Chr6:86620367-86620386 | None:intergenic | 50.0% | |
| CCGTTAACGGGAGTAGCATT+AGG | - | Chr6:86619765-86619784 | None:intergenic | 50.0% | |
| CGTTCCTCACCGCCAAAAAA+CGG | - | Chr6:86620251-86620270 | None:intergenic | 50.0% | |
| CTCCGGGAAATCCGGATTAT+CGG | + | Chr6:86620209-86620228 | MsG0680034380.01.T01:CDS | 50.0% | |
| CTTTATGCCGTAGACTCTGC+AGG | + | Chr6:86619970-86619989 | MsG0680034380.01.T01:CDS | 50.0% | |
| GATATCGATGCTCCGGTGTA+AGG | - | Chr6:86620031-86620050 | None:intergenic | 50.0% | |
| GCATCGTGGTAGATTGAGGA+AGG | - | Chr6:86619951-86619970 | None:intergenic | 50.0% | |
| GGAAATCCGGATTATCGGGT+TGG | + | Chr6:86620214-86620233 | MsG0680034380.01.T01:CDS | 50.0% | |
| GGCACATCATCTTGCAGTTC+GGG | + | Chr6:86620172-86620191 | MsG0680034380.01.T01:CDS | 50.0% | |
| GGTTACTTCTCCGGTAACAG+TGG | - | Chr6:86619850-86619869 | None:intergenic | 50.0% | |
| GTCCTTACCATCGACACCAA+CGG | - | Chr6:86620305-86620324 | None:intergenic | 50.0% | |
| GTTAAAGAGACGGCGGTTGA+CGG | - | Chr6:86619721-86619740 | None:intergenic | 50.0% | |
| TCCGGGAAATCCGGATTATC+GGG | + | Chr6:86620210-86620229 | MsG0680034380.01.T01:CDS | 50.0% | |
| TGTCAACTACCGCCTTACAC+CGG | + | Chr6:86620016-86620035 | MsG0680034380.01.T01:CDS | 50.0% | |
| TGTCGATGGTAAGGACGGAT+TGG | + | Chr6:86620309-86620328 | MsG0680034380.01.T01:CDS | 50.0% | |
| TTGGTGGAAACGCCGTTAAC+GGG | - | Chr6:86619777-86619796 | None:intergenic | 50.0% | |
| ! | CTGGCTTTCCAAGGAAGAAG+AGG | - | Chr6:86619642-86619661 | None:intergenic | 50.0% |
| ! | GACAGGAAGGGAGGTAGTTT+TGG | - | Chr6:86619889-86619908 | None:intergenic | 50.0% |
| ! | GCAGTTCGGGTTTGTAAAGC+TGG | + | Chr6:86620185-86620204 | MsG0680034380.01.T01:CDS | 50.0% |
| !! | ATGGTAGCTCTGGCTTTCCA+AGG | - | Chr6:86619651-86619670 | None:intergenic | 50.0% |
| !! | GTCTACGGCATAAAGCATCG+TGG | - | Chr6:86619965-86619984 | None:intergenic | 50.0% |
| !! | GTGGTGGAAGGTGTTCTTAC+CGG | + | Chr6:86620331-86620350 | MsG0680034380.01.T01:CDS | 50.0% |
| !! | GTTGGTGTCGATGGTAAGGA+CGG | + | Chr6:86620304-86620323 | MsG0680034380.01.T01:CDS | 50.0% |
| !! | TGGAAGGTGTTCTTACCGGA+GGG | + | Chr6:86620335-86620354 | MsG0680034380.01.T01:CDS | 50.0% |
| !!! | CGTTTTTTGGCGGTGAGGAA+CGG | + | Chr6:86620249-86620268 | MsG0680034380.01.T01:CDS | 50.0% |
| ACCGGAGAAGTAACCGGAGA+CGG | + | Chr6:86619855-86619874 | MsG0680034380.01.T01:CDS | 55.0% | |
| ACCGTCTCCGGTTACTTCTC+CGG | - | Chr6:86619859-86619878 | None:intergenic | 55.0% | |
| ATCGATGCTCCGGTGTAAGG+CGG | - | Chr6:86620028-86620047 | None:intergenic | 55.0% | |
| CCCGACAAATCCTCCGCATT+CGG | - | Chr6:86620398-86620417 | None:intergenic | 55.0% | |
| CCGAATGCGGAGGATTTGTC+GGG | + | Chr6:86620395-86620414 | MsG0680034380.01.T01:CDS | 55.0% | |
| GAATGTAAGCGGGCCGAATG+CGG | + | Chr6:86620382-86620401 | MsG0680034380.01.T01:CDS | 55.0% | |
| GCCGAATGCGGAGGATTTGT+CGG | + | Chr6:86620394-86620413 | MsG0680034380.01.T01:CDS | 55.0% | |
| GGCGCTTCTTCTCGCTCTAA+CGG | + | Chr6:86619693-86619712 | MsG0680034380.01.T01:CDS | 55.0% | |
| GGGTTTGTAAAGCTGGGCTC+CGG | + | Chr6:86620192-86620211 | MsG0680034380.01.T01:CDS | 55.0% | |
| GGTTTGTAAAGCTGGGCTCC+GGG | + | Chr6:86620193-86620212 | MsG0680034380.01.T01:CDS | 55.0% | |
| TCATTTACTTCCACGGCGGC+GGG | + | Chr6:86619910-86619929 | MsG0680034380.01.T01:CDS | 55.0% | |
| TGCGACGGATATCGATGCTC+CGG | - | Chr6:86620038-86620057 | None:intergenic | 55.0% | |
| TGCGGAGGATTTGTCGGGTT+TGG | + | Chr6:86620400-86620419 | MsG0680034380.01.T01:CDS | 55.0% | |
| TTCCTTGCCGGAGACAGTTC+CGG | + | Chr6:86620140-86620159 | MsG0680034380.01.T01:CDS | 55.0% | |
| TTTGGTTGCACCACCGTCTC+CGG | - | Chr6:86619871-86619890 | None:intergenic | 55.0% | |
| ! | TGATCTCGGTTCGATCCCTC+CGG | - | Chr6:86620353-86620372 | None:intergenic | 55.0% |
| !! | AAGGACGGATTGGTGGTGGA+AGG | + | Chr6:86620319-86620338 | MsG0680034380.01.T01:CDS | 55.0% |
| !! | CGATGGTAAGGACGGATTGG+TGG | + | Chr6:86620312-86620331 | MsG0680034380.01.T01:CDS | 55.0% |
| !! | GTGGAAGGTGTTCTTACCGG+AGG | + | Chr6:86620334-86620353 | MsG0680034380.01.T01:CDS | 55.0% |
| !! | TGGTAAGGACGGATTGGTGG+TGG | + | Chr6:86620315-86620334 | MsG0680034380.01.T01:CDS | 55.0% |
| !!! | TCAGCCGTTTTTTGGCGGTG+AGG | + | Chr6:86620244-86620263 | MsG0680034380.01.T01:CDS | 55.0% |
| AAGCTGGGCTCCGGGAAATC+CGG | + | Chr6:86620201-86620220 | MsG0680034380.01.T01:CDS | 60.0% | |
| GGAGAAGTAACCGGAGACGG+TGG | + | Chr6:86619858-86619877 | MsG0680034380.01.T01:CDS | 60.0% | |
| !! | CGCCGTTGGTGTCGATGGTA+AGG | + | Chr6:86620300-86620319 | MsG0680034380.01.T01:CDS | 60.0% |
| !! | TGGATCGCCGTTGGTGTCGA+TGG | + | Chr6:86620295-86620314 | MsG0680034380.01.T01:CDS | 60.0% |
| CACCGGAACTGTCTCCGGCA+AGG | - | Chr6:86620145-86620164 | None:intergenic | 65.0% | |
| GAAAACTGAACCCGCCGCCG+TGG | - | Chr6:86619923-86619942 | None:intergenic | 65.0% | |
| TGTAAGCGGGCCGAATGCGG+AGG | + | Chr6:86620385-86620404 | MsG0680034380.01.T01:CDS | 65.0% | |
| CGGTGAGGAACGGACCGAGG+CGG | + | Chr6:86620259-86620278 | MsG0680034380.01.T01:CDS | 70.0% | |
| TGGCGGTGAGGAACGGACCG+AGG | + | Chr6:86620256-86620275 | MsG0680034380.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 86619624 | 86620640 | 86619624 | ID=MsG0680034380.01;Name=MsG0680034380.01 |
| Chr6 | mRNA | 86619624 | 86620640 | 86619624 | ID=MsG0680034380.01.T01;Parent=MsG0680034380.01;Name=MsG0680034380.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|338 |
| Chr6 | exon | 86619624 | 86620640 | 86619624 | ID=MsG0680034380.01.T01:exon:18204;Parent=MsG0680034380.01.T01 |
| Chr6 | CDS | 86619624 | 86620640 | 86619624 | ID=MsG0680034380.01.T01:cds;Parent=MsG0680034380.01.T01 |
| Gene Sequence |
| Protein sequence |
