AlfalfaGEDB | MsG0280010141.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010141.01

KEGG: K05577
GO: -
PFAM: PF01010

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010141.01.T01	RHN50267.1	92.424	66	5	0	4	69	96	161	7.56E-35	126

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010141.01.T01	P15958	84.848	66	10	0	4	69	646	711	1.18E-33	123

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010141.01.T01	A0A396HH97	92.424	66	5	0	4	69	96	161	3.61e-35	126

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010141.01	MsG0380013922.01	0.821534	3.836252e-53	3.129341e-50
MsG0280010141.01	MsG0780037769.01	0.835611	1.517117e-56	1.864806e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010141.01.T01	MTR_4g051300	92.424	66	5	0	4	69	646	711	3.74e-35	125
MsG0280010141.01.T01	MTR_7g021740	92.424	66	5	0	4	69	676	741	4.15e-35	125

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010141.01.T01	ATCG01010	59.677	62	25	0	8	69	646	707	1.86e-20	83.2

Find 8 sgRNAs with CRISPR-Local

Find 9 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGGAATTCCAAATGGAAT+TGG	0.315379	2:+65294881	MsG0280010141.01.T01:CDS
TTTGATAGACAAGTAATTGA+TGG	0.319357	2:+65294863	MsG0280010141.01.T01:CDS
CTGGAAATCCCAATTCCATT+TGG	0.377156	2:-65294890	None:intergenic
ATGGAATTCCAAATGGAATT+GGG	0.401786	2:+65294882	MsG0280010141.01.T01:CDS
TAATCAATGTTATATATGAT+TGG	0.429297	2:+65294756	MsG0280010141.01.T01:CDS
TTTAATTGCGAGTGTAAGAA+AGG	0.472458	2:+65294820	MsG0280010141.01.T01:CDS
GTAATTGATGGAATTCCAAA+TGG	0.479083	2:+65294875	MsG0280010141.01.T01:CDS
TATGATTGGTCATATAATCG+TGG	0.650873	2:+65294770	MsG0280010141.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATCAATGTTATATATGAT+TGG	+	Chr2:65294756-65294775	MsG0280010141.01.T01:CDS	15.0%
!!!	TTATCAGCAATAATTTTTTG+AGG	-	Chr2:65294734-65294753	None:intergenic	20.0%
!	TTTGATAGACAAGTAATTGA+TGG	+	Chr2:65294863-65294882	MsG0280010141.01.T01:CDS	25.0%
	ATGGAATTCCAAATGGAATT+GGG	+	Chr2:65294882-65294901	MsG0280010141.01.T01:CDS	30.0%
	GTAATTGATGGAATTCCAAA+TGG	+	Chr2:65294875-65294894	MsG0280010141.01.T01:CDS	30.0%
	TTTAATTGCGAGTGTAAGAA+AGG	+	Chr2:65294820-65294839	MsG0280010141.01.T01:CDS	30.0%
!	TATGATTGGTCATATAATCG+TGG	+	Chr2:65294770-65294789	MsG0280010141.01.T01:CDS	30.0%
	GATGGAATTCCAAATGGAAT+TGG	+	Chr2:65294881-65294900	MsG0280010141.01.T01:CDS	35.0%
	CTGGAAATCCCAATTCCATT+TGG	-	Chr2:65294893-65294912	None:intergenic	40.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	65294713	65294922	65294713	ID=MsG0280010141.01;Name=MsG0280010141.01
Chr2	mRNA	65294713	65294922	65294713	ID=MsG0280010141.01.T01;Parent=MsG0280010141.01;Name=MsG0280010141.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|69
Chr2	exon	65294713	65294922	65294713	ID=MsG0280010141.01.T01:exon:18063;Parent=MsG0280010141.01.T01
Chr2	CDS	65294713	65294922	65294713	ID=MsG0280010141.01.T01:cds;Parent=MsG0280010141.01.T01

Gene Sequence	>MsG0280010141.01.T01 TTTATTTCAAAAAAGTGTCCTCAAAAAATTATTGCTGATAAAATAATCAATGTTATATATGATTGGTCATATAATCGTGGTTATATAGATGCTTTTTTTGAAGTATCTTTAATTGCGAGTGTAAGAAAGGTAGCTAAATTCAATTATTTTTTTGATAGACAAGTAATTGATGGAATTCCAAATGGAATTGGGATTTCCAGTTTTTTTTAG
Protein sequence	>MsG0280010141.01.T01 FISKKCPQKIIADKIINVIYDWSYNRGYIDAFFEVSLIASVRKVAKFNYFFDRQVIDGIPNGIGISSFF*

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