Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037769.01.T01 | YP_001381723.1 | 95.062 | 81 | 4 | 0 | 1 | 81 | 1 | 81 | 2.54E-44 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037769.01.T01 | P08212 | 92.593 | 81 | 6 | 0 | 1 | 81 | 1 | 81 | 4.22E-46 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037769.01.T01 | A0A4P8F405 | 95.062 | 81 | 4 | 0 | 1 | 81 | 1 | 81 | 1.21e-44 | 147 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002436.01 | MsG0780037769.01 | 0.806862 | 6.692600e-50 | 3.672446e-47 |
| MsG0380013922.01 | MsG0780037769.01 | 0.885495 | 7.744289e-72 | 5.327215e-68 |
| MsG0580027686.01 | MsG0780037769.01 | 0.845454 | 4.020070e-59 | 6.702704e-56 |
| MsG0280010141.01 | MsG0780037769.01 | 0.835611 | 1.517117e-56 | 1.864806e-53 |
| MsG0780037769.01 | MsG0780037869.01 | 0.828740 | 7.597662e-55 | 7.617827e-52 |
| MsG0780037769.01 | MsG0780037989.01 | 0.815240 | 1.022881e-51 | 7.013708e-49 |
| MsG0780037769.01 | MsG0780038139.01 | 0.833867 | 4.165823e-56 | 4.859995e-53 |
| MsG0780037769.01 | MsG0880044963.01 | 0.850460 | 1.674280e-60 | 3.281965e-57 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037769.01.T01 | MTR_4g051097 | 95.062 | 81 | 4 | 0 | 1 | 81 | 1 | 81 | 3.08e-48 | 147 |
| MsG0780037769.01.T01 | MTR_0002s0540 | 93.617 | 47 | 3 | 0 | 1 | 47 | 1 | 47 | 5.92e-24 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037769.01.T01 | ATCG00140 | 91.358 | 81 | 7 | 0 | 1 | 81 | 1 | 81 | 4.56e-46 | 142 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGAAGCTTTAACTATTTA+TGG | 0.037973 | 7:-32963031 | MsG0780037769.01.T01:CDS |
| CGGAAGCAGAGGACAAAATA+AGG | 0.270738 | 7:-32963081 | MsG0780037769.01.T01:CDS |
| GGGTACTTTATTACTTAGTA+TGG | 0.275552 | 7:-32963059 | MsG0780037769.01.T01:CDS |
| GCCGTCGGGCTTGCTTCTAT+TGG | 0.311843 | 7:-32963163 | MsG0780037769.01.T01:CDS |
| GCTGCTTCTGTTATCGCTGC+TGG | 0.315135 | 7:-32963190 | MsG0780037769.01.T01:CDS |
| TGGAAGCTTTAACTATTTAT+GGG | 0.316470 | 7:-32963030 | MsG0780037769.01.T01:CDS |
| GCTACAGGGCAAGCTGTAGA+AGG | 0.370271 | 7:-32963118 | MsG0780037769.01.T01:CDS |
| GCTTCTATTGGACCTGGAGT+TGG | 0.416315 | 7:-32963151 | MsG0780037769.01.T01:CDS |
| TCGCTGCTGGGTTGGCCGTC+GGG | 0.427770 | 7:-32963177 | MsG0780037769.01.T01:CDS |
| ATCGCTGCTGGGTTGGCCGT+CGG | 0.428270 | 7:-32963178 | MsG0780037769.01.T01:CDS |
| AGCTTTAACTATTTATGGGC+TGG | 0.460263 | 7:-32963026 | MsG0780037769.01.T01:CDS |
| CTGCTTCTGTTATCGCTGCT+GGG | 0.461552 | 7:-32963189 | MsG0780037769.01.T01:CDS |
| GTTGGTCAAGGTACAGCTAC+AGG | 0.473074 | 7:-32963133 | MsG0780037769.01.T01:CDS |
| GGAAGCAGAGGACAAAATAA+GGG | 0.473459 | 7:-32963080 | MsG0780037769.01.T01:CDS |
| AGAAGGGATTGCGCGACAAC+CGG | 0.475077 | 7:-32963101 | MsG0780037769.01.T01:CDS |
| GGGCTTGCTTCTATTGGACC+TGG | 0.481695 | 7:-32963157 | MsG0780037769.01.T01:CDS |
| TTCTGTTATCGCTGCTGGGT+TGG | 0.514112 | 7:-32963185 | MsG0780037769.01.T01:CDS |
| ACAGAAGCAGCAGAAATAAG+TGG | 0.523712 | 7:+32963202 | None:intergenic |
| ATTGGACCTGGAGTTGGTCA+AGG | 0.543278 | 7:-32963145 | MsG0780037769.01.T01:CDS |
| TGCGCGACAACCGGAAGCAG+AGG | 0.554171 | 7:-32963092 | MsG0780037769.01.T01:CDS |
| CTACAGGGCAAGCTGTAGAA+GGG | 0.590645 | 7:-32963117 | MsG0780037769.01.T01:CDS |
| GCTGTACCTTGACCAACTCC+AGG | 0.593501 | 7:+32963139 | None:intergenic |
| TCCAATAGAAGCAAGCCCGA+CGG | 0.638086 | 7:+32963162 | None:intergenic |
| TTGGTCAAGGTACAGCTACA+GGG | 0.640991 | 7:-32963132 | MsG0780037769.01.T01:CDS |
| GAAGCAGAGGACAAAATAAG+GGG | 0.658398 | 7:-32963079 | MsG0780037769.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGATTAAACAAAAAGGAT+TGG | + | Chr7:32963208-32963227 | None:intergenic | 20.0% |
| !! | ATGGAAGCTTTAACTATTTA+TGG | - | Chr7:32963153-32963172 | MsG0780037769.01.T01:CDS | 25.0% |
| !! | ATTACTTAGTATGGCTTTTA+TGG | - | Chr7:32963134-32963153 | MsG0780037769.01.T01:CDS | 25.0% |
| !! | TGGAAGCTTTAACTATTTAT+GGG | - | Chr7:32963154-32963173 | MsG0780037769.01.T01:CDS | 25.0% |
| GGGTACTTTATTACTTAGTA+TGG | - | Chr7:32963125-32963144 | MsG0780037769.01.T01:CDS | 30.0% | |
| ! | AGCTTTAACTATTTATGGGC+TGG | - | Chr7:32963158-32963177 | MsG0780037769.01.T01:CDS | 35.0% |
| ACAGAAGCAGCAGAAATAAG+TGG | + | Chr7:32962985-32963004 | None:intergenic | 40.0% | |
| GAAGCAGAGGACAAAATAAG+GGG | - | Chr7:32963105-32963124 | MsG0780037769.01.T01:CDS | 40.0% | |
| GGAAGCAGAGGACAAAATAA+GGG | - | Chr7:32963104-32963123 | MsG0780037769.01.T01:CDS | 40.0% | |
| !!! | CTTATTTTGTCCTCTGCTTC+CGG | + | Chr7:32963105-32963124 | None:intergenic | 40.0% |
| CGGAAGCAGAGGACAAAATA+AGG | - | Chr7:32963103-32963122 | MsG0780037769.01.T01:CDS | 45.0% | |
| TTGGTCAAGGTACAGCTACA+GGG | - | Chr7:32963052-32963071 | MsG0780037769.01.T01:CDS | 45.0% | |
| CTACAGGGCAAGCTGTAGAA+GGG | - | Chr7:32963067-32963086 | MsG0780037769.01.T01:CDS | 50.0% | |
| CTGCTTCTGTTATCGCTGCT+GGG | - | Chr7:32962995-32963014 | MsG0780037769.01.T01:CDS | 50.0% | |
| GCTTCTATTGGACCTGGAGT+TGG | - | Chr7:32963033-32963052 | MsG0780037769.01.T01:CDS | 50.0% | |
| GTTGGTCAAGGTACAGCTAC+AGG | - | Chr7:32963051-32963070 | MsG0780037769.01.T01:CDS | 50.0% | |
| TCCAATAGAAGCAAGCCCGA+CGG | + | Chr7:32963025-32963044 | None:intergenic | 50.0% | |
| TTCTGTTATCGCTGCTGGGT+TGG | - | Chr7:32962999-32963018 | MsG0780037769.01.T01:CDS | 50.0% | |
| ! | ATTGGACCTGGAGTTGGTCA+AGG | - | Chr7:32963039-32963058 | MsG0780037769.01.T01:CDS | 50.0% |
| AGAAGGGATTGCGCGACAAC+CGG | - | Chr7:32963083-32963102 | MsG0780037769.01.T01:CDS | 55.0% | |
| GCTACAGGGCAAGCTGTAGA+AGG | - | Chr7:32963066-32963085 | MsG0780037769.01.T01:CDS | 55.0% | |
| GCTGCTTCTGTTATCGCTGC+TGG | - | Chr7:32962994-32963013 | MsG0780037769.01.T01:CDS | 55.0% | |
| GCTGTACCTTGACCAACTCC+AGG | + | Chr7:32963048-32963067 | None:intergenic | 55.0% | |
| ! | GGGCTTGCTTCTATTGGACC+TGG | - | Chr7:32963027-32963046 | MsG0780037769.01.T01:CDS | 55.0% |
| ! | GCCGTCGGGCTTGCTTCTAT+TGG | - | Chr7:32963021-32963040 | MsG0780037769.01.T01:CDS | 60.0% |
| TGCGCGACAACCGGAAGCAG+AGG | - | Chr7:32963092-32963111 | MsG0780037769.01.T01:CDS | 65.0% | |
| !! | ATCGCTGCTGGGTTGGCCGT+CGG | - | Chr7:32963006-32963025 | MsG0780037769.01.T01:CDS | 65.0% |
| !! | TCGCTGCTGGGTTGGCCGTC+GGG | - | Chr7:32963007-32963026 | MsG0780037769.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 32962976 | 32963230 | 32962976 | ID=MsG0780037769.01;Name=MsG0780037769.01 |
| Chr7 | mRNA | 32962976 | 32963230 | 32962976 | ID=MsG0780037769.01.T01;Parent=MsG0780037769.01;Name=MsG0780037769.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|84 |
| Chr7 | exon | 32962976 | 32963230 | 32962976 | ID=MsG0780037769.01.T01:exon:1120;Parent=MsG0780037769.01.T01 |
| Chr7 | CDS | 32962976 | 32963230 | 32962976 | ID=MsG0780037769.01.T01:cds;Parent=MsG0780037769.01.T01 |
| Gene Sequence |
| Protein sequence |
