Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013922.01.T01 | QFG71255.1 | 96.774 | 31 | 1 | 0 | 1 | 31 | 103 | 133 | 4.25E-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013922.01.T01 | Q2PMQ0 | 96.774 | 31 | 1 | 0 | 1 | 31 | 103 | 133 | 1.56E-13 | 63.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013922.01.T01 | A0A5J6VB21 | 96.774 | 31 | 1 | 0 | 1 | 31 | 103 | 133 | 2.03e-11 | 64.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013922.01 | MsG0580024908.01 | 0.851638 | 7.789481e-61 | 1.586763e-57 |
| MsG0380013922.01 | MsG0580027686.01 | 0.848358 | 6.449138e-60 | 1.180438e-56 |
| MsG0380013922.01 | MsG0680031180.01 | 0.809522 | 1.815363e-50 | 1.068223e-47 |
| MsG0380013922.01 | MsG0780037769.01 | 0.885495 | 7.744289e-72 | 5.327215e-68 |
| MsG0380013922.01 | MsG0780037869.01 | 0.856940 | 2.289644e-62 | 5.556237e-59 |
| MsG0380013922.01 | MsG0780038139.01 | 0.867706 | 1.122996e-65 | 3.957941e-62 |
| MsG0380013922.01 | MsG0880044963.01 | 0.835886 | 1.292044e-56 | 1.601328e-53 |
| MsG0280010141.01 | MsG0380013922.01 | 0.821534 | 3.836252e-53 | 3.129341e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013922.01.T01 | MTR_4g059910 | 96.774 | 31 | 1 | 0 | 1 | 31 | 111 | 141 | 9.21e-15 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013922.01.T01 | ATCG00770 | 83.871 | 31 | 5 | 0 | 1 | 31 | 103 | 133 | 1.70e-12 | 58.2 |
Find 9 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTATATACATTATTGTAAA+TGG | 0.151969 | 3:-48415677 | MsG0380013922.01.T01:CDS |
| TTGTAATTCTTTCTACTTCT+AGG | 0.233849 | 3:-48415806 | MsG0380013922.01.T01:CDS |
| TGTAATTCTTTCTACTTCTA+GGG | 0.394272 | 3:-48415805 | MsG0380013922.01.T01:CDS |
| ATAGAAATCAAATCGGATAT+CGG | 0.408827 | 3:+48415705 | None:intergenic |
| TAAATGGAGTAGAGAAGTAA+TGG | 0.513988 | 3:-48415661 | MsG0380013922.01.T01:CDS |
| GAGGCTCGATTAGAAAGAAT+CGG | 0.553245 | 3:-48415765 | MsG0380013922.01.T01:CDS |
| GTAATTCTTTCTACTTCTAG+GGG | 0.588562 | 3:-48415804 | MsG0380013922.01.T01:CDS |
| GGGTATAATGACAGATCGAG+AGG | 0.665637 | 3:-48415784 | MsG0380013922.01.T01:CDS |
| GCTCGATTAGAAAGAATCGG+CGG | 0.737146 | 3:-48415762 | MsG0380013922.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATATACATTATTGTAAA+TGG | - | Chr3:48415777-48415796 | MsG0380013922.01.T01:CDS | 10.0% |
| !!! | ATTTTTCATAGAAATCAAAT+CGG | + | Chr3:48415759-48415778 | None:intergenic | 15.0% |
| !!! | AGTTTTATGTTATATGGTAA+TGG | - | Chr3:48415718-48415737 | MsG0380013922.01.T01:CDS | 20.0% |
| ! | ATAGAAATCAAATCGGATAT+CGG | + | Chr3:48415752-48415771 | None:intergenic | 25.0% |
| ! | TGTAATTCTTTCTACTTCTA+GGG | - | Chr3:48415649-48415668 | MsG0380013922.01.T01:CDS | 25.0% |
| ! | TTGTAATTCTTTCTACTTCT+AGG | - | Chr3:48415648-48415667 | MsG0380013922.01.T01:CDS | 25.0% |
| GTAATTCTTTCTACTTCTAG+GGG | - | Chr3:48415650-48415669 | MsG0380013922.01.T01:CDS | 30.0% | |
| TAAATGGAGTAGAGAAGTAA+TGG | - | Chr3:48415793-48415812 | MsG0380013922.01.T01:CDS | 30.0% | |
| !! | CGGCGAAGTTTTATGTTATA+TGG | - | Chr3:48415712-48415731 | MsG0380013922.01.T01:CDS | 35.0% |
| GAGGCTCGATTAGAAAGAAT+CGG | - | Chr3:48415689-48415708 | MsG0380013922.01.T01:CDS | 40.0% | |
| GCTCGATTAGAAAGAATCGG+CGG | - | Chr3:48415692-48415711 | MsG0380013922.01.T01:CDS | 45.0% | |
| GGGTATAATGACAGATCGAG+AGG | - | Chr3:48415670-48415689 | MsG0380013922.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48415641 | 48415835 | 48415641 | ID=MsG0380013922.01;Name=MsG0380013922.01 |
| Chr3 | mRNA | 48415641 | 48415835 | 48415641 | ID=MsG0380013922.01.T01;Parent=MsG0380013922.01;Name=MsG0380013922.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|64 |
| Chr3 | exon | 48415641 | 48415835 | 48415641 | ID=MsG0380013922.01.T01:exon:9430;Parent=MsG0380013922.01.T01 |
| Chr3 | CDS | 48415641 | 48415835 | 48415641 | ID=MsG0380013922.01.T01:cds;Parent=MsG0380013922.01.T01 |
| Gene Sequence |
| Protein sequence |
