AlfalfaGEDB | MsG0280010192.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010192.01

KEGG: -
GO: -
PFAM: PF05678

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010192.01.T01	XP_013464690.1	99.533	214	1	0	1	214	33	246	5.01E-147	419

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010192.01.T01	Q5M750	58.373	209	65	10	16	209	37	238	1.95E-59	189

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010192.01.T01	B7FK40	99.533	214	1	0	1	214	33	246	2.39e-147	419

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002971.01	MsG0280010192.01	0.800983	1.115796e-48	5.268097e-46
MsG0280008258.01	MsG0280010192.01	0.803870	2.836219e-49	1.441236e-46
MsG0280010192.01	MsG0380012008.01	0.810164	1.320404e-50	7.902186e-48
MsG0280010192.01	MsG0380018026.01	0.818051	2.397796e-52	1.775723e-49
MsG0280010192.01	MsG0780039450.01	0.827723	1.336685e-54	1.301172e-51
MsG0280010192.01	MsG0780041589.01	0.849797	2.567135e-60	4.923752e-57
MsG0280010192.01	MsG0880042368.01	0.810409	1.169442e-50	7.043493e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010192.01.T01	MTR_2g079120	99.533	214	1	0	1	214	33	246	6.06e-151	419
MsG0280010192.01.T01	MTR_2g013950	55.450	211	58	8	12	209	65	252	1.14e-55	178
MsG0280010192.01.T01	MTR_4g088695	52.041	196	62	7	28	209	56	233	1.36e-43	146

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010192.01.T01	AT1G28280	58.571	210	65	10	16	210	37	239	4.78e-61	191
MsG0280010192.01.T01	AT1G28280	58.373	209	65	10	16	209	37	238	1.99e-60	189
MsG0280010192.01.T01	AT3G15300	56.398	211	56	11	18	210	24	216	3.27e-54	173
MsG0280010192.01.T01	AT5G53830	55.392	204	59	8	28	207	44	239	2.90e-52	169
MsG0280010192.01.T01	AT2G33780	39.362	188	72	7	31	205	34	192	5.85e-28	105
MsG0280010192.01.T01	AT1G80450	34.536	194	79	9	33	213	15	173	8.15e-14	67.4
MsG0280010192.01.T01	AT5G08480	34.637	179	91	5	33	210	17	170	5.24e-11	59.7
MsG0280010192.01.T01	AT5G08480	34.637	179	91	5	33	210	17	170	5.24e-11	59.7

Find 40 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAGAGGTGGTGTTGGAATT+TGG	0.212600	2:+65967674	None:intergenic
AATTTGAAACCTTGTTTCTT+TGG	0.221923	2:+65967433	None:intergenic
GATAAATCTTCACCTTCATT+AGG	0.311360	2:-65967229	MsG0280010192.01.T01:CDS
GCATCTCTTGGTGTAGTAAT+AGG	0.315866	2:+65967148	None:intergenic
TGTTGGTTTGGTTTCGTTGC+TGG	0.324991	2:+65967511	None:intergenic
CAAGTAGTTCAAATGTTAAC+TGG	0.330596	2:-65967574	MsG0280010192.01.T01:CDS
TGAACAAAGGTTGTTGGATA+AGG	0.368483	2:+65967619	None:intergenic
GAAGGTGAAGATTTATCAAA+AGG	0.403207	2:+65967235	None:intergenic
GTTGAGGTAGATCTTGTTGT+TGG	0.407347	2:+65967494	None:intergenic
GGTAGATCTTGTTGTTGGTT+TGG	0.410465	2:+65967499	None:intergenic
ATAAGGATTTGAATCAGATC+TGG	0.420109	2:+65967636	None:intergenic
TCATCATTCAATGGTGTGAC+AGG	0.420771	2:+65967259	None:intergenic
TCTGATGAGTTTCCTAATGA+AGG	0.454202	2:+65967217	None:intergenic
GAAGGAAAATCAAGCAAGCT+TGG	0.463885	2:+65967295	None:intergenic
AGTTGTGGCTCTGCATCTCT+TGG	0.480249	2:+65967136	None:intergenic
TCAAAAGGGTCATCATTCAA+TGG	0.482624	2:+65967250	None:intergenic
TTTGGAGTGAGAGGTGGTGT+TGG	0.496921	2:+65967667	None:intergenic
GACAAAGCAATTGCTGAAAG+AGG	0.510604	2:-65967190	MsG0280010192.01.T01:CDS
TCAGCTTGAACAAAGGTTGT+TGG	0.515758	2:+65967613	None:intergenic
TAAGGATTTGAATCAGATCT+GGG	0.517218	2:+65967637	None:intergenic
AATTTGGATTCCATTGCTGT+TGG	0.517362	2:+65967690	None:intergenic
TTGAACTACTTGTTTGAAAG+TGG	0.519842	2:+65967585	None:intergenic
TGTTGTTGTTGGTGTTGTTG+AGG	0.524736	2:+65967537	None:intergenic
CTTGGTGTAGTAATAGGAGA+GGG	0.527748	2:+65967154	None:intergenic
AAGGTGAAGATTTATCAAAA+GGG	0.535615	2:+65967236	None:intergenic
GAATTTGATTGTGAAACTCT+AGG	0.540707	2:+65967088	None:intergenic
TTAAAGTTTCTTGTTTGTTG+AGG	0.548499	2:+65967478	None:intergenic
TGTTGTTGAGGTTGTTTCTG+AGG	0.570694	2:+65967549	None:intergenic
GGTGAAGAAAGAGGAAACAA+AGG	0.573082	2:+65967109	None:intergenic
GGTTGTGTCAGCTTGAACAA+AGG	0.574058	2:+65967606	None:intergenic
GGAAACAAAGGAAGAAGTTG+TGG	0.576455	2:+65967121	None:intergenic
AGTAGCAATTCCAACAGCAA+TGG	0.578466	2:-65967700	MsG0280010192.01.T01:CDS
GAAACTCTAGGTGAAGAAAG+AGG	0.626680	2:+65967100	None:intergenic
CATCATTCAATGGTGTGACA+GGG	0.632245	2:+65967260	None:intergenic
TCTTGGTGTAGTAATAGGAG+AGG	0.640047	2:+65967153	None:intergenic
ACAGGGCTAAGAGCAAGAGA+AGG	0.663065	2:+65967277	None:intergenic
TTCATTAGGAAACTCATCAG+AGG	0.664188	2:-65967215	MsG0280010192.01.T01:CDS
AAAACAGCACCAAAGAAACA+AGG	0.675794	2:-65967442	MsG0280010192.01.T01:CDS
AAGGATTTGAATCAGATCTG+GGG	0.682451	2:+65967638	None:intergenic
TTGGTGTAGTAATAGGAGAG+GGG	0.712277	2:+65967155	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGGTGAAGATTTATCAAAA+GGG	+	Chr2:65967557-65967576	None:intergenic	25.0%
!	TTGGAATTCTGTGAAAAATT+TGG	+	Chr2:65967438-65967457	None:intergenic	25.0%
!!	AATTTGAAACCTTGTTTCTT+TGG	+	Chr2:65967360-65967379	None:intergenic	25.0%
!!	TTAAAGTTTCTTGTTTGTTG+AGG	+	Chr2:65967315-65967334	None:intergenic	25.0%
!!!	ATTTCTTGTTGTTGTTTTCT+TGG	+	Chr2:65967468-65967487	None:intergenic	25.0%
	ATAAGGATTTGAATCAGATC+TGG	+	Chr2:65967157-65967176	None:intergenic	30.0%
	CAAGTAGTTCAAATGTTAAC+TGG	-	Chr2:65967216-65967235	MsG0280010192.01.T01:CDS	30.0%
	GAAGGTGAAGATTTATCAAA+AGG	+	Chr2:65967558-65967577	None:intergenic	30.0%
	GAATTTGATTGTGAAACTCT+AGG	+	Chr2:65967705-65967724	None:intergenic	30.0%
	GATAAATCTTCACCTTCATT+AGG	-	Chr2:65967561-65967580	MsG0280010192.01.T01:CDS	30.0%
	TAAGGATTTGAATCAGATCT+GGG	+	Chr2:65967156-65967175	None:intergenic	30.0%
	TTGAACTACTTGTTTGAAAG+TGG	+	Chr2:65967208-65967227	None:intergenic	30.0%
!	TTGTTTTCTTGGTGAGAAAT+TGG	+	Chr2:65967457-65967476	None:intergenic	30.0%
	AAAACAGCACCAAAGAAACA+AGG	-	Chr2:65967348-65967367	MsG0280010192.01.T01:CDS	35.0%
	AAGGATTTGAATCAGATCTG+GGG	+	Chr2:65967155-65967174	None:intergenic	35.0%
	AATTTGGATTCCATTGCTGT+TGG	+	Chr2:65967103-65967122	None:intergenic	35.0%
	CTGTGAAAAATTTGGCATCA+GGG	+	Chr2:65967430-65967449	None:intergenic	35.0%
	TCAAAAGGGTCATCATTCAA+TGG	+	Chr2:65967543-65967562	None:intergenic	35.0%
	TCTGATGAGTTTCCTAATGA+AGG	+	Chr2:65967576-65967595	None:intergenic	35.0%
	TCTGTGAAAAATTTGGCATC+AGG	+	Chr2:65967431-65967450	None:intergenic	35.0%
	TGAACAAAGGTTGTTGGATA+AGG	+	Chr2:65967174-65967193	None:intergenic	35.0%
	TTCATTAGGAAACTCATCAG+AGG	-	Chr2:65967575-65967594	MsG0280010192.01.T01:CDS	35.0%
!!!	TTCTTTGGTGCTGTTTTGAT+TGG	+	Chr2:65967345-65967364	None:intergenic	35.0%
	AGTAGCAATTCCAACAGCAA+TGG	-	Chr2:65967090-65967109	MsG0280010192.01.T01:CDS	40.0%
	CATCATTCAATGGTGTGACA+GGG	+	Chr2:65967533-65967552	None:intergenic	40.0%
	GAAACTCTAGGTGAAGAAAG+AGG	+	Chr2:65967693-65967712	None:intergenic	40.0%
	GAAGGAAAATCAAGCAAGCT+TGG	+	Chr2:65967498-65967517	None:intergenic	40.0%
	GACAAAGCAATTGCTGAAAG+AGG	-	Chr2:65967600-65967619	MsG0280010192.01.T01:CDS	40.0%
	GGAAACAAAGGAAGAAGTTG+TGG	+	Chr2:65967672-65967691	None:intergenic	40.0%
	GGTGAAGAAAGAGGAAACAA+AGG	+	Chr2:65967684-65967703	None:intergenic	40.0%
	TCAGCTTGAACAAAGGTTGT+TGG	+	Chr2:65967180-65967199	None:intergenic	40.0%
	TCATCATTCAATGGTGTGAC+AGG	+	Chr2:65967534-65967553	None:intergenic	40.0%
	TTGGTGTAGTAATAGGAGAG+GGG	+	Chr2:65967638-65967657	None:intergenic	40.0%
!	CTTGGTGTAGTAATAGGAGA+GGG	+	Chr2:65967639-65967658	None:intergenic	40.0%
!	GCATCTCTTGGTGTAGTAAT+AGG	+	Chr2:65967645-65967664	None:intergenic	40.0%
!	GTTGAGGTAGATCTTGTTGT+TGG	+	Chr2:65967299-65967318	None:intergenic	40.0%
!	TCAGATCTGGGGATTGTTTT+TGG	+	Chr2:65967144-65967163	None:intergenic	40.0%
!	TCTTGGTGTAGTAATAGGAG+AGG	+	Chr2:65967640-65967659	None:intergenic	40.0%
!!	GGTAGATCTTGTTGTTGGTT+TGG	+	Chr2:65967294-65967313	None:intergenic	40.0%
!!	TGTTGTTGAGGTTGTTTCTG+AGG	+	Chr2:65967244-65967263	None:intergenic	40.0%
!!	TGTTGTTGTTGGTGTTGTTG+AGG	+	Chr2:65967256-65967275	None:intergenic	40.0%
!!!	ATTGTTTTTGGAGTGAGAGG+TGG	+	Chr2:65967132-65967151	None:intergenic	40.0%
!!!	GTTGCTGGTTTTGTTGTTGT+TGG	+	Chr2:65967267-65967286	None:intergenic	40.0%
!!!	TTGGTGCTGTTTTGATTGGT+GGG	+	Chr2:65967341-65967360	None:intergenic	40.0%
!!!	TTTGGTGCTGTTTTGATTGG+TGG	+	Chr2:65967342-65967361	None:intergenic	40.0%
	GGTTGTGTCAGCTTGAACAA+AGG	+	Chr2:65967187-65967206	None:intergenic	45.0%
!	TGAGAGGTGGTGTTGGAATT+TGG	+	Chr2:65967119-65967138	None:intergenic	45.0%
!!	TGTTGGTTTGGTTTCGTTGC+TGG	+	Chr2:65967282-65967301	None:intergenic	45.0%
!!!	GGGATTGTTTTTGGAGTGAG+AGG	+	Chr2:65967135-65967154	None:intergenic	45.0%
	ACAGGGCTAAGAGCAAGAGA+AGG	+	Chr2:65967516-65967535	None:intergenic	50.0%
!	AGTTGTGGCTCTGCATCTCT+TGG	+	Chr2:65967657-65967676	None:intergenic	50.0%
!	TTTGGAGTGAGAGGTGGTGT+TGG	+	Chr2:65967126-65967145	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	65967084	65967728	65967084	ID=MsG0280010192.01;Name=MsG0280010192.01
Chr2	mRNA	65967084	65967728	65967084	ID=MsG0280010192.01.T01;Parent=MsG0280010192.01;Name=MsG0280010192.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|214
Chr2	exon	65967084	65967728	65967084	ID=MsG0280010192.01.T01:exon:5198;Parent=MsG0280010192.01.T01
Chr2	CDS	65967084	65967728	65967084	ID=MsG0280010192.01.T01:cds;Parent=MsG0280010192.01.T01

Gene Sequence

>MsG0280010192.01.T01

ATGAGTAGTAGCAATTCCAACAGCAATGGAATCCAAATTCCAACACCACCTCTCACTCCAAAAACAATCCCCAGATCTGATTCAAATCCTTATCCAACAACCTTTGTTCAAGCTGACACAACCACTTTCAAACAAGTAGTTCAAATGTTAACTGGTTCCTCAGAAACAACCTCAACAACACCAACAACAACAAAACCAGCAACGAAACCAAACCAACAACAAGATCTACCTCAACAAACAAGAAACTTTAACATCCCACCAATCAAAACAGCACCAAAGAAACAAGGTTTCAAATTATATGAAAGAAGAAACAGTCTCAAAAACAGTGTTCTTATGCTCAATACCCTGATGCCAAATTTTTCACAGAATTCCAATTTCTCACCAAGAAAACAACAACAAGAAATTCTCTCTCCAAGCTTGCTTGATTTTCCTTCTCTTGCTCTTAGCCCTGTCACACCATTGAATGATGACCCTTTTGATAAATCTTCACCTTCATTAGGAAACTCATCAGAGGAAGACAAAGCAATTGCTGAAAGAGGTTACTATTTGCACCCCTCTCCTATTACTACACCAAGAGATGCAGAGCCACAACTTCTTCCTTTGTTTCCTCTTTCTTCACCTAGAGTTTCACAATCAAATTCTTGA

Protein sequence

>MsG0280010192.01.T01

MSSSNSNSNGIQIPTPPLTPKTIPRSDSNPYPTTFVQADTTTFKQVVQMLTGSSETTSTTPTTTKPATKPNQQQDLPQQTRNFNIPPIKTAPKKQGFKLYERRNSLKNSVLMLNTLMPNFSQNSNFSPRKQQQEILSPSLLDFPSLALSPVTPLNDDPFDKSSPSLGNSSEEDKAIAERGYYLHPSPITTPRDAEPQLLPLFPLSSPRVSQSNS*