Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002971.01.T03 | XP_024633540.1 | 95.327 | 214 | 9 | 1 | 1 | 214 | 1 | 213 | 5.92E-125 | 362 |
| MsG0180002971.01.T02 | XP_024633540.1 | 88.626 | 211 | 9 | 2 | 1 | 197 | 4 | 213 | 3.82E-108 | 318 |
| MsG0180002971.01.T01 | XP_024633540.1 | 90.521 | 211 | 9 | 2 | 1 | 201 | 4 | 213 | 1.64E-110 | 325 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002971.01.T01 | A0A396JPW1 | 90.521 | 211 | 9 | 2 | 1 | 201 | 4 | 213 | 7.83e-111 | 325 |
| MsG0180002971.01.T02 | A0A396JPW1 | 88.626 | 211 | 9 | 2 | 1 | 197 | 4 | 213 | 1.83e-108 | 318 |
| MsG0180002971.01.T03 | A0A396JPW1 | 95.327 | 214 | 9 | 1 | 1 | 214 | 1 | 213 | 2.83e-125 | 362 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048302.01 | MsG0180002971.01 | 0.810774 | 9.751463e-51 | 5.931178e-48 |
| MsG0180002971.01 | MsG0180003130.01 | 0.810542 | 1.094285e-50 | 6.614348e-48 |
| MsG0180002971.01 | MsG0280008258.01 | 0.837472 | 5.095516e-57 | 6.626100e-54 |
| MsG0180002971.01 | MsG0280010192.01 | 0.800983 | 1.115796e-48 | 5.268097e-46 |
| MsG0180002971.01 | MsG0380018026.01 | 0.819456 | 1.150376e-52 | 8.857364e-50 |
| MsG0180002971.01 | MsG0780041589.01 | 0.817078 | 3.972824e-52 | 2.864826e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0180002971.01 | MsG0180002971.01 | PPI |
| MsG0180002971.01 | MsG0180002971.01 | PPI |
| MsG0180002971.01 | MsG0180002971.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002971.01.T03 | MTR_1g053900 | 94.686 | 207 | 11 | 0 | 1 | 207 | 1 | 207 | 9.91e-125 | 352 |
| MsG0180002971.01.T03 | MTR_7g093040 | 57.674 | 215 | 62 | 10 | 4 | 214 | 1 | 190 | 1.52e-62 | 193 |
| MsG0180002971.01.T02 | MTR_1g053900 | 87.745 | 204 | 11 | 1 | 1 | 190 | 4 | 207 | 7.81e-108 | 308 |
| MsG0180002971.01.T02 | MTR_7g093040 | 55.660 | 212 | 57 | 10 | 1 | 197 | 1 | 190 | 7.12e-57 | 178 |
| MsG0180002971.01.T01 | MTR_1g053900 | 89.706 | 204 | 11 | 1 | 1 | 194 | 4 | 207 | 2.49e-110 | 315 |
| MsG0180002971.01.T01 | MTR_7g093040 | 56.190 | 210 | 63 | 9 | 1 | 201 | 1 | 190 | 3.67e-57 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002971.01.T03 | AT3G57950 | 40.541 | 185 | 81 | 7 | 36 | 214 | 20 | 181 | 5.92e-29 | 107 |
| MsG0180002971.01.T03 | AT2G42180 | 37.500 | 184 | 81 | 6 | 35 | 214 | 13 | 166 | 3.18e-26 | 99.8 |
| MsG0180002971.01.T03 | AT5G06790 | 41.848 | 184 | 80 | 10 | 36 | 204 | 29 | 200 | 5.33e-25 | 97.8 |
| MsG0180002971.01.T02 | AT3G57950 | 43.860 | 171 | 81 | 7 | 33 | 197 | 20 | 181 | 5.73e-31 | 111 |
| MsG0180002971.01.T02 | AT2G42180 | 39.080 | 174 | 78 | 6 | 32 | 197 | 13 | 166 | 3.55e-26 | 99.0 |
| MsG0180002971.01.T02 | AT5G06790 | 39.891 | 183 | 71 | 10 | 33 | 187 | 29 | 200 | 1.97e-23 | 93.2 |
| MsG0180002971.01.T01 | AT3G57950 | 42.857 | 175 | 81 | 7 | 33 | 201 | 20 | 181 | 1.29e-30 | 110 |
| MsG0180002971.01.T01 | AT5G06790 | 40.782 | 179 | 79 | 8 | 33 | 191 | 29 | 200 | 1.08e-25 | 99.0 |
| MsG0180002971.01.T01 | AT2G42180 | 37.931 | 174 | 84 | 5 | 32 | 201 | 13 | 166 | 1.40e-24 | 95.1 |
Find 44 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTAGGCTTCACTATAAT+TGG | 0.085592 | 1:-54077552 | MsG0180002971.01.T02:CDS |
| CAAATTGTCATGAGAAGTTT+AGG | 0.211400 | 1:-54077273 | MsG0180002971.01.T02:CDS |
| AGACTATGAGAGTGTGAATT+AGG | 0.264505 | 1:+54077719 | None:intergenic |
| TCACTTAAAGTAACGGCTTT+AGG | 0.298398 | 1:+54077466 | None:intergenic |
| AGATCATTATGGGATCATTT+AGG | 0.320073 | 1:-54077567 | MsG0180002971.01.T02:CDS |
| ATTGATGGTGATGGTTATAA+AGG | 0.360443 | 1:-54077355 | MsG0180002971.01.T02:CDS |
| GTCTTATAGAAGGAGGTATC+AGG | 0.369094 | 1:-54077236 | MsG0180002971.01.T02:CDS |
| CTCACCATTGTCGTGACTCT+TGG | 0.385889 | 1:+54077438 | None:intergenic |
| ATTTAGGCTTCACTATAATT+GGG | 0.394444 | 1:-54077551 | MsG0180002971.01.T02:CDS |
| AACTAAAGATGATTTGACTT+TGG | 0.397354 | 1:+54077666 | None:intergenic |
| ATCTTTAGTTGAGATTCTTA+AGG | 0.400989 | 1:-54077653 | MsG0180002971.01.T02:CDS |
| GGGTAAGGGACATGAATATT+GGG | 0.404339 | 1:+54077622 | None:intergenic |
| AGGGTAAGGGACATGAATAT+TGG | 0.412723 | 1:+54077621 | None:intergenic |
| AAATTGTCATGAGAAGTTTA+GGG | 0.419624 | 1:-54077272 | MsG0180002971.01.T02:CDS |
| TGAGAAGTTTAGGGTTGAAA+AGG | 0.422153 | 1:-54077263 | MsG0180002971.01.T02:CDS |
| TTCTTCTCATGTTATTATGC+CGG | 0.434051 | 1:-54077515 | MsG0180002971.01.T02:CDS |
| GAAGTTTAGGGTTGAAAAGG+AGG | 0.456704 | 1:-54077260 | MsG0180002971.01.T02:CDS |
| CTGAAGATAACTAGCAAGTT+CGG | 0.465642 | 1:+54077399 | None:intergenic |
| AAAAGGAGGAGTCTTATAGA+AGG | 0.467140 | 1:-54077246 | MsG0180002971.01.T02:CDS |
| TATTAAGGATGTTGATATGT+TGG | 0.493773 | 1:-54077311 | MsG0180002971.01.T02:CDS |
| GGTATCAGGAAATGTTGGTT+AGG | 0.502738 | 1:-54077222 | MsG0180002971.01.T02:CDS |
| TTTAGTGAGTAAATTGGAAG+AGG | 0.503327 | 1:+54077793 | None:intergenic |
| GAGGAGAATGATGATATTGA+TGG | 0.505465 | 1:-54077370 | MsG0180002971.01.T02:CDS |
| TGGATAGTGCACGAATAATA+CGG | 0.507108 | 1:+54077686 | None:intergenic |
| ATAAAGGGAGTGAAAAGGAG+GGG | 0.508325 | 1:-54077339 | MsG0180002971.01.T02:CDS |
| TTGATGGTGATGGTTATAAA+GGG | 0.509890 | 1:-54077354 | MsG0180002971.01.T02:CDS |
| AAGGAGGTATCAGGAAATGT+TGG | 0.520204 | 1:-54077227 | MsG0180002971.01.T02:CDS |
| GGCTTGTTCACTTAAAGTAA+CGG | 0.529335 | 1:+54077459 | None:intergenic |
| AACTTGCTAGTTATCTTCAG+TGG | 0.548810 | 1:-54077396 | MsG0180002971.01.T02:CDS |
| TTAGTGAGTAAATTGGAAGA+GGG | 0.549248 | 1:+54077794 | None:intergenic |
| TAGTTATCTTCAGTGGCTTG+AGG | 0.552802 | 1:-54077389 | MsG0180002971.01.T02:CDS |
| AGGAGGAGTCTTATAGAAGG+AGG | 0.564681 | 1:-54077243 | MsG0180002971.01.T02:CDS |
| TGTGAGGTGTTGCGATGAGA+AGG | 0.571330 | 1:+54077817 | None:intergenic |
| TTATAAAGGGAGTGAAAAGG+AGG | 0.576667 | 1:-54077341 | MsG0180002971.01.T02:CDS |
| TAAAGGGAGTGAAAAGGAGG+GGG | 0.590072 | 1:-54077338 | MsG0180002971.01.T02:CDS |
| TCGAAAACTCGAGATGGCAC+CGG | 0.590800 | 1:+54077496 | None:intergenic |
| AATGATGATATTGATGGTGA+TGG | 0.591684 | 1:-54077364 | MsG0180002971.01.T02:CDS |
| TGGTTATAAAGGGAGTGAAA+AGG | 0.593105 | 1:-54077344 | MsG0180002971.01.T02:CDS |
| AACCCTTCGAAAACTCGAGA+TGG | 0.618099 | 1:+54077490 | None:intergenic |
| TATAAAGGGAGTGAAAAGGA+GGG | 0.637935 | 1:-54077340 | MsG0180002971.01.T02:CDS |
| GTGAGGTGTTGCGATGAGAA+GGG | 0.662946 | 1:+54077818 | None:intergenic |
| CAAGAGTCACGACAATGGTG+AGG | 0.671465 | 1:-54077437 | MsG0180002971.01.T02:CDS |
| GTAAATTGGAAGAGGGTGTG+AGG | 0.691454 | 1:+54077801 | None:intergenic |
| CAAGCCAAGAGTCACGACAA+TGG | 0.693114 | 1:-54077442 | MsG0180002971.01.T02:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAAAAAAGATCATTAT+GGG | - | Chr1:54077471-54077490 | MsG0180002971.01.T02:intron | 15.0% |
| !! | CAAAACAAAAAAGATCATTA+TGG | - | Chr1:54077470-54077489 | MsG0180002971.01.T02:intron | 20.0% |
| !!! | AATTAGGTTTTTAATCTTTG+TGG | + | Chr1:54077316-54077335 | None:intergenic | 20.0% |
| !!! | TAGAATGTTTTTTATGTGAA+GGG | + | Chr1:54077449-54077468 | None:intergenic | 20.0% |
| ! | AAATTGTCATGAGAAGTTTA+GGG | - | Chr1:54077776-54077795 | MsG0180002971.01.T02:CDS | 25.0% |
| ! | ATCTTTAGTTGAGATTCTTA+AGG | - | Chr1:54077395-54077414 | MsG0180002971.01.T02:CDS | 25.0% |
| ! | ATTTAGGCTTCACTATAATT+GGG | - | Chr1:54077497-54077516 | MsG0180002971.01.T02:intron | 25.0% |
| ! | TATTAAGGATGTTGATATGT+TGG | - | Chr1:54077737-54077756 | MsG0180002971.01.T02:CDS | 25.0% |
| !! | AACTAAAGATGATTTGACTT+TGG | + | Chr1:54077385-54077404 | None:intergenic | 25.0% |
| !!! | CTAGAATGTTTTTTATGTGA+AGG | + | Chr1:54077450-54077469 | None:intergenic | 25.0% |
| !!! | GATGATTTTAGTGAGTAAAT+TGG | + | Chr1:54077264-54077283 | None:intergenic | 25.0% |
| AGATCATTATGGGATCATTT+AGG | - | Chr1:54077481-54077500 | MsG0180002971.01.T02:intron | 30.0% | |
| CAAATTGTCATGAGAAGTTT+AGG | - | Chr1:54077775-54077794 | MsG0180002971.01.T02:CDS | 30.0% | |
| CATTTAGGCTTCACTATAAT+TGG | - | Chr1:54077496-54077515 | MsG0180002971.01.T02:intron | 30.0% | |
| TTAGTGAGTAAATTGGAAGA+GGG | + | Chr1:54077257-54077276 | None:intergenic | 30.0% | |
| TTCTTCTCATGTTATTATGC+CGG | - | Chr1:54077533-54077552 | MsG0180002971.01.T02:CDS | 30.0% | |
| TTTAGTGAGTAAATTGGAAG+AGG | + | Chr1:54077258-54077277 | None:intergenic | 30.0% | |
| ! | TAGGTTTTTAATCTTTGTGG+AGG | + | Chr1:54077313-54077332 | None:intergenic | 30.0% |
| !! | AATGATGATATTGATGGTGA+TGG | - | Chr1:54077684-54077703 | MsG0180002971.01.T02:CDS | 30.0% |
| !! | ATTGATGGTGATGGTTATAA+AGG | - | Chr1:54077693-54077712 | MsG0180002971.01.T02:CDS | 30.0% |
| !! | TTGATGGTGATGGTTATAAA+GGG | - | Chr1:54077694-54077713 | MsG0180002971.01.T02:CDS | 30.0% |
| !!! | GTTTTTTATGTGAAGGGTAA+GGG | + | Chr1:54077443-54077462 | None:intergenic | 30.0% |
| !!! | TGTTTTTTATGTGAAGGGTA+AGG | + | Chr1:54077444-54077463 | None:intergenic | 30.0% |
| CTGAAGATAACTAGCAAGTT+CGG | + | Chr1:54077652-54077671 | None:intergenic | 35.0% | |
| GAGGAGAATGATGATATTGA+TGG | - | Chr1:54077678-54077697 | MsG0180002971.01.T02:CDS | 35.0% | |
| TATAAAGGGAGTGAAAAGGA+GGG | - | Chr1:54077708-54077727 | MsG0180002971.01.T02:CDS | 35.0% | |
| TCACTTAAAGTAACGGCTTT+AGG | + | Chr1:54077585-54077604 | None:intergenic | 35.0% | |
| TGGATAGTGCACGAATAATA+CGG | + | Chr1:54077365-54077384 | None:intergenic | 35.0% | |
| TGGTTATAAAGGGAGTGAAA+AGG | - | Chr1:54077704-54077723 | MsG0180002971.01.T02:CDS | 35.0% | |
| TTATAAAGGGAGTGAAAAGG+AGG | - | Chr1:54077707-54077726 | MsG0180002971.01.T02:CDS | 35.0% | |
| ! | AAAAGGAGGAGTCTTATAGA+AGG | - | Chr1:54077802-54077821 | MsG0180002971.01.T02:CDS | 35.0% |
| ! | AGACTATGAGAGTGTGAATT+AGG | + | Chr1:54077332-54077351 | None:intergenic | 35.0% |
| ! | GGCTTGTTCACTTAAAGTAA+CGG | + | Chr1:54077592-54077611 | None:intergenic | 35.0% |
| ! | TGAGAAGTTTAGGGTTGAAA+AGG | - | Chr1:54077785-54077804 | MsG0180002971.01.T02:CDS | 35.0% |
| !! | AACTTGCTAGTTATCTTCAG+TGG | - | Chr1:54077652-54077671 | MsG0180002971.01.T02:CDS | 35.0% |
| AGGGTAAGGGACATGAATAT+TGG | + | Chr1:54077430-54077449 | None:intergenic | 40.0% | |
| ATAAAGGGAGTGAAAAGGAG+GGG | - | Chr1:54077709-54077728 | MsG0180002971.01.T02:CDS | 40.0% | |
| GGGTAAGGGACATGAATATT+GGG | + | Chr1:54077429-54077448 | None:intergenic | 40.0% | |
| TAGTTATCTTCAGTGGCTTG+AGG | - | Chr1:54077659-54077678 | MsG0180002971.01.T02:CDS | 40.0% | |
| ! | GAAGTTTAGGGTTGAAAAGG+AGG | - | Chr1:54077788-54077807 | MsG0180002971.01.T02:CDS | 40.0% |
| ! | GGTATCAGGAAATGTTGGTT+AGG | - | Chr1:54077826-54077845 | MsG0180002971.01.T02:CDS | 40.0% |
| !! | AAGGAGGTATCAGGAAATGT+TGG | - | Chr1:54077821-54077840 | MsG0180002971.01.T02:CDS | 40.0% |
| !! | GTCTTATAGAAGGAGGTATC+AGG | - | Chr1:54077812-54077831 | MsG0180002971.01.T02:CDS | 40.0% |
| AAAGGAGGGGGAGACTATTA+AGG | - | Chr1:54077722-54077741 | MsG0180002971.01.T02:CDS | 45.0% | |
| AACCCTTCGAAAACTCGAGA+TGG | + | Chr1:54077561-54077580 | None:intergenic | 45.0% | |
| TAAAGGGAGTGAAAAGGAGG+GGG | - | Chr1:54077710-54077729 | MsG0180002971.01.T02:CDS | 45.0% | |
| ! | AGGAGGAGTCTTATAGAAGG+AGG | - | Chr1:54077805-54077824 | MsG0180002971.01.T02:CDS | 45.0% |
| ! | GTAAATTGGAAGAGGGTGTG+AGG | + | Chr1:54077250-54077269 | None:intergenic | 45.0% |
| ! | TGCCATCTCGAGTTTTCGAA+GGG | - | Chr1:54077556-54077575 | MsG0180002971.01.T02:CDS | 45.0% |
| CAAGAGTCACGACAATGGTG+AGG | - | Chr1:54077611-54077630 | MsG0180002971.01.T02:CDS | 50.0% | |
| CAAGCCAAGAGTCACGACAA+TGG | - | Chr1:54077606-54077625 | MsG0180002971.01.T02:CDS | 50.0% | |
| CTCACCATTGTCGTGACTCT+TGG | + | Chr1:54077613-54077632 | None:intergenic | 50.0% | |
| TCGAAAACTCGAGATGGCAC+CGG | + | Chr1:54077555-54077574 | None:intergenic | 50.0% | |
| ! | GTGCCATCTCGAGTTTTCGA+AGG | - | Chr1:54077555-54077574 | MsG0180002971.01.T02:CDS | 50.0% |
| !! | GTGAGGTGTTGCGATGAGAA+GGG | + | Chr1:54077233-54077252 | None:intergenic | 50.0% |
| !! | TGTGAGGTGTTGCGATGAGA+AGG | + | Chr1:54077234-54077253 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 54077213 | 54077857 | 54077213 | ID=MsG0180002971.01;Name=MsG0180002971.01 |
| Chr1 | mRNA | 54077213 | 54077848 | 54077213 | ID=MsG0180002971.01.T01;Parent=MsG0180002971.01;Name=MsG0180002971.01.T01;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|0|0|2|0|201 |
| Chr1 | exon | 54077213 | 54077493 | 54077213 | ID=MsG0180002971.01.T01:exon:47847;Parent=MsG0180002971.01.T01 |
| Chr1 | exon | 54077524 | 54077848 | 54077524 | ID=MsG0180002971.01.T01:exon:47846;Parent=MsG0180002971.01.T01 |
| Chr1 | CDS | 54077524 | 54077848 | 54077524 | ID=MsG0180002971.01.T01:cds;Parent=MsG0180002971.01.T01 |
| Chr1 | CDS | 54077213 | 54077493 | 54077213 | ID=MsG0180002971.01.T01:cds;Parent=MsG0180002971.01.T01 |
| Chr1 | mRNA | 54077213 | 54077848 | 54077213 | ID=MsG0180002971.01.T02;Parent=MsG0180002971.01;Name=MsG0180002971.01.T02;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|0|0|2|0|197 |
| Chr1 | exon | 54077213 | 54077468 | 54077213 | ID=MsG0180002971.01.T02:exon:47849;Parent=MsG0180002971.01.T02 |
| Chr1 | exon | 54077511 | 54077848 | 54077511 | ID=MsG0180002971.01.T02:exon:47848;Parent=MsG0180002971.01.T02 |
| Chr1 | CDS | 54077511 | 54077848 | 54077511 | ID=MsG0180002971.01.T02:cds;Parent=MsG0180002971.01.T02 |
| Chr1 | CDS | 54077213 | 54077468 | 54077213 | ID=MsG0180002971.01.T02:cds;Parent=MsG0180002971.01.T02 |
| Chr1 | mRNA | 54077213 | 54077857 | 54077213 | ID=MsG0180002971.01.T03;Parent=MsG0180002971.01;Name=MsG0180002971.01.T03;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|214 |
| Chr1 | exon | 54077213 | 54077857 | 54077213 | ID=MsG0180002971.01.T03:exon:47850;Parent=MsG0180002971.01.T03 |
| Chr1 | CDS | 54077213 | 54077857 | 54077213 | ID=MsG0180002971.01.T03:cds;Parent=MsG0180002971.01.T03 |
| Gene Sequence |
| Protein sequence |
