Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008258.01.T01 | XP_003595045.1 | 98.907 | 183 | 2 | 0 | 1 | 183 | 1 | 183 | 7.95E-131 | 374 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008258.01.T01 | Q2HUM6 | 98.907 | 183 | 2 | 0 | 1 | 183 | 1 | 183 | 3.80e-131 | 374 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002971.01 | MsG0280008258.01 | 0.837472 | 5.095516e-57 | 6.626100e-54 |
| MsG0280006433.01 | MsG0280008258.01 | 0.801705 | 7.937402e-49 | 3.816184e-46 |
| MsG0280008117.01 | MsG0280008258.01 | 0.830686 | 2.554112e-55 | 2.710675e-52 |
| MsG0280008258.01 | MsG0280010192.01 | 0.803870 | 2.836219e-49 | 1.441236e-46 |
| MsG0280008258.01 | MsG0380016643.01 | 0.823577 | 1.284962e-53 | 1.110765e-50 |
| MsG0280008258.01 | MsG0380018026.01 | 0.850901 | 1.257799e-60 | 2.501540e-57 |
| MsG0280008258.01 | MsG0480019895.01 | 0.815187 | 1.051448e-51 | 7.198941e-49 |
| MsG0280008258.01 | MsG0480020219.01 | 0.862044 | 6.697532e-64 | 1.934488e-60 |
| MsG0280008258.01 | MsG0480022099.01 | 0.802939 | 4.421977e-49 | 2.193853e-46 |
| MsG0280008258.01 | MsG0480022520.01 | 0.811652 | 6.290637e-51 | 3.915679e-48 |
| MsG0280008258.01 | MsG0580024343.01 | 0.823697 | 1.204665e-53 | 1.045062e-50 |
| MsG0280008258.01 | MsG0580025683.01 | 0.822406 | 2.409405e-53 | 2.014998e-50 |
| MsG0280008258.01 | MsG0580029555.01 | 0.826081 | 3.298679e-54 | 3.062218e-51 |
| MsG0280008258.01 | MsG0680031429.01 | 0.833792 | 4.350059e-56 | 5.063384e-53 |
| MsG0280008258.01 | MsG0680034852.01 | 0.823639 | 1.243103e-53 | 1.076499e-50 |
| MsG0280008258.01 | MsG0780039450.01 | 0.806247 | 9.023398e-50 | 4.874090e-47 |
| MsG0280008258.01 | MsG0780041380.01 | 0.820719 | 5.913125e-53 | 4.715132e-50 |
| MsG0280008258.01 | MsG0880044669.01 | 0.800717 | 1.264217e-48 | 5.928164e-46 |
| MsG0280008258.01 | MsG0880045861.01 | 0.822312 | 2.533760e-53 | 2.113231e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008258.01.T01 | MTR_2g037760 | 98.907 | 183 | 2 | 0 | 1 | 183 | 1 | 183 | 9.63e-135 | 374 |
| MsG0280008258.01.T01 | MTR_4g127530 | 57.838 | 185 | 70 | 4 | 1 | 182 | 1 | 180 | 3.15e-66 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATGCTTTGAGCAGTATT+TGG | 0.233848 | 2:+28115926 | MsG0280008258.01.T01:CDS |
| CGATGTTTCGCCTGTGGATT+TGG | 0.273838 | 2:+28115813 | MsG0280008258.01.T01:CDS |
| ATCACTTGGTTAACTGAAAC+TGG | 0.314102 | 2:-28115652 | None:intergenic |
| AAAGCATCTTGGTTTGATAA+AGG | 0.331144 | 2:-28115913 | None:intergenic |
| GCACATGTAGAGGGCGGTTA+TGG | 0.354136 | 2:+28116019 | MsG0280008258.01.T01:CDS |
| TCGGCTTGTAGAATTGAATA+AGG | 0.390315 | 2:-28115793 | None:intergenic |
| AGCATGAAGTAAACATTTCC+TGG | 0.401282 | 2:-28115769 | None:intergenic |
| TTCGGCCACTGCAACTCTGC+CGG | 0.411739 | 2:-28115968 | None:intergenic |
| CACATCAAAGAAAATCCGTT+TGG | 0.427399 | 2:+28115594 | MsG0280008258.01.T01:CDS |
| GCGGTTATGGAGACCTCTAT+TGG | 0.447733 | 2:+28116032 | MsG0280008258.01.T01:CDS |
| ATCCGTTTGGTTCATCTAAG+TGG | 0.450947 | 2:+28115607 | MsG0280008258.01.T01:CDS |
| AAATACTGCTCAAAGCATCT+TGG | 0.454917 | 2:-28115924 | None:intergenic |
| AGTTGTGTATCTCCTTTCAA+TGG | 0.456894 | 2:-28115742 | None:intergenic |
| TCATTGATATTCTTTCAAAT+CGG | 0.458020 | 2:-28116094 | None:intergenic |
| AAGAAGATGAAAGAAGTTGA+AGG | 0.463012 | 2:-28115714 | None:intergenic |
| GATTTCTACTATTGATCACT+TGG | 0.465741 | 2:-28115666 | None:intergenic |
| TCGGCCACTGCAACTCTGCC+GGG | 0.472425 | 2:-28115967 | None:intergenic |
| ATTTGGCTTCGCTTGCGAAA+AGG | 0.473956 | 2:+28115830 | MsG0280008258.01.T01:CDS |
| TGCGAAAAGGCTCACTGCAA+AGG | 0.520433 | 2:+28115843 | MsG0280008258.01.T01:CDS |
| ACAAGCCGATGTTTCGCCTG+TGG | 0.520762 | 2:+28115807 | MsG0280008258.01.T01:CDS |
| TCTTCTTCAAAACCATTGAA+AGG | 0.529265 | 2:+28115730 | MsG0280008258.01.T01:CDS |
| ATACGTCATACCGTATTGTT+CGG | 0.544090 | 2:-28115986 | None:intergenic |
| GAACAATACGGTATGACGTA+TGG | 0.550185 | 2:+28115988 | MsG0280008258.01.T01:CDS |
| AGAAGATGAAAGAAGTTGAA+GGG | 0.551501 | 2:-28115713 | None:intergenic |
| AGTTCGCCTTCGATGAGTCC+CGG | 0.573481 | 2:+28115949 | MsG0280008258.01.T01:CDS |
| GTACAAACAAAGTGAGTTGG+TGG | 0.573498 | 2:-28115688 | None:intergenic |
| GTTGCAGTGGCCGAACAATA+CGG | 0.575627 | 2:+28115976 | MsG0280008258.01.T01:CDS |
| ACTCTGCCGGGACTCATCGA+AGG | 0.577322 | 2:-28115955 | None:intergenic |
| CAAATCCACAGGCGAAACAT+CGG | 0.579625 | 2:-28115812 | None:intergenic |
| GAGGTACAAACAAAGTGAGT+TGG | 0.588726 | 2:-28115691 | None:intergenic |
| TCATCTAAGTGGTTATGTAG+AGG | 0.591667 | 2:+28115618 | MsG0280008258.01.T01:CDS |
| GAGTCCCGGCAGAGTTGCAG+TGG | 0.594628 | 2:+28115963 | MsG0280008258.01.T01:CDS |
| TCTCTGATAGTATCCAATAG+AGG | 0.601725 | 2:-28116045 | None:intergenic |
| AACCACTTAGATGAACCAAA+CGG | 0.602554 | 2:-28115609 | None:intergenic |
| GCAAGCGAAGCCAAATCCAC+AGG | 0.607715 | 2:-28115823 | None:intergenic |
| AGATGAAAGAAGTTGAAGGG+AGG | 0.610782 | 2:-28115710 | None:intergenic |
| GGAGGACGAAGCACATGTAG+AGG | 0.624245 | 2:+28116009 | MsG0280008258.01.T01:CDS |
| GAGGACGAAGCACATGTAGA+GGG | 0.636486 | 2:+28116010 | MsG0280008258.01.T01:CDS |
| CAATACGGTATGACGTATGG+AGG | 0.653418 | 2:+28115991 | MsG0280008258.01.T01:CDS |
| GACGAAGCACATGTAGAGGG+CGG | 0.686792 | 2:+28116013 | MsG0280008258.01.T01:CDS |
| GGAGATACACAACTTCAACC+AGG | 0.693853 | 2:+28115751 | MsG0280008258.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATTGATATTCTTTCAAAT+CGG | - | Chr2:28116097-28116116 | None:intergenic | 20.0% |
| AAGAAGATGAAAGAAGTTGA+AGG | - | Chr2:28115717-28115736 | None:intergenic | 30.0% | |
| AGAAGATGAAAGAAGTTGAA+GGG | - | Chr2:28115716-28115735 | None:intergenic | 30.0% | |
| GATGAGAAAAAGTCATTGAA+AGG | + | Chr2:28115883-28115902 | MsG0280008258.01.T01:CDS | 30.0% | |
| GATTTCTACTATTGATCACT+TGG | - | Chr2:28115669-28115688 | None:intergenic | 30.0% | |
| TCTTCTTCAAAACCATTGAA+AGG | + | Chr2:28115730-28115749 | MsG0280008258.01.T01:CDS | 30.0% | |
| ! | AAAGCATCTTGGTTTGATAA+AGG | - | Chr2:28115916-28115935 | None:intergenic | 30.0% |
| AACCACTTAGATGAACCAAA+CGG | - | Chr2:28115612-28115631 | None:intergenic | 35.0% | |
| AGCATGAAGTAAACATTTCC+TGG | - | Chr2:28115772-28115791 | None:intergenic | 35.0% | |
| AGTTGTGTATCTCCTTTCAA+TGG | - | Chr2:28115745-28115764 | None:intergenic | 35.0% | |
| ATACGTCATACCGTATTGTT+CGG | - | Chr2:28115989-28116008 | None:intergenic | 35.0% | |
| ATCACTTGGTTAACTGAAAC+TGG | - | Chr2:28115655-28115674 | None:intergenic | 35.0% | |
| CACATCAAAGAAAATCCGTT+TGG | + | Chr2:28115594-28115613 | MsG0280008258.01.T01:CDS | 35.0% | |
| TCATCTAAGTGGTTATGTAG+AGG | + | Chr2:28115618-28115637 | MsG0280008258.01.T01:CDS | 35.0% | |
| TCTCTGATAGTATCCAATAG+AGG | - | Chr2:28116048-28116067 | None:intergenic | 35.0% | |
| ! | AAATACTGCTCAAAGCATCT+TGG | - | Chr2:28115927-28115946 | None:intergenic | 35.0% |
| ! | AAGATGCTTTGAGCAGTATT+TGG | + | Chr2:28115926-28115945 | MsG0280008258.01.T01:CDS | 35.0% |
| ! | TCGGCTTGTAGAATTGAATA+AGG | - | Chr2:28115796-28115815 | None:intergenic | 35.0% |
| AGATGAAAGAAGTTGAAGGG+AGG | - | Chr2:28115713-28115732 | None:intergenic | 40.0% | |
| GAACAATACGGTATGACGTA+TGG | + | Chr2:28115988-28116007 | MsG0280008258.01.T01:CDS | 40.0% | |
| GAGGTACAAACAAAGTGAGT+TGG | - | Chr2:28115694-28115713 | None:intergenic | 40.0% | |
| ! | ATCCGTTTGGTTCATCTAAG+TGG | + | Chr2:28115607-28115626 | MsG0280008258.01.T01:CDS | 40.0% |
| ! | GTACAAACAAAGTGAGTTGG+TGG | - | Chr2:28115691-28115710 | None:intergenic | 40.0% |
| CAAATCCACAGGCGAAACAT+CGG | - | Chr2:28115815-28115834 | None:intergenic | 45.0% | |
| CAATACGGTATGACGTATGG+AGG | + | Chr2:28115991-28116010 | MsG0280008258.01.T01:CDS | 45.0% | |
| GGAGATACACAACTTCAACC+AGG | + | Chr2:28115751-28115770 | MsG0280008258.01.T01:CDS | 45.0% | |
| ! | ATTTGGCTTCGCTTGCGAAA+AGG | + | Chr2:28115830-28115849 | MsG0280008258.01.T01:CDS | 45.0% |
| CGATGTTTCGCCTGTGGATT+TGG | + | Chr2:28115813-28115832 | MsG0280008258.01.T01:CDS | 50.0% | |
| GAGGACGAAGCACATGTAGA+GGG | + | Chr2:28116010-28116029 | MsG0280008258.01.T01:CDS | 50.0% | |
| GCGGTTATGGAGACCTCTAT+TGG | + | Chr2:28116032-28116051 | MsG0280008258.01.T01:CDS | 50.0% | |
| GTTGCAGTGGCCGAACAATA+CGG | + | Chr2:28115976-28115995 | MsG0280008258.01.T01:CDS | 50.0% | |
| TGCGAAAAGGCTCACTGCAA+AGG | + | Chr2:28115843-28115862 | MsG0280008258.01.T01:CDS | 50.0% | |
| ACAAGCCGATGTTTCGCCTG+TGG | + | Chr2:28115807-28115826 | MsG0280008258.01.T01:CDS | 55.0% | |
| AGTTCGCCTTCGATGAGTCC+CGG | + | Chr2:28115949-28115968 | MsG0280008258.01.T01:CDS | 55.0% | |
| GACGAAGCACATGTAGAGGG+CGG | + | Chr2:28116013-28116032 | MsG0280008258.01.T01:CDS | 55.0% | |
| GCAAGCGAAGCCAAATCCAC+AGG | - | Chr2:28115826-28115845 | None:intergenic | 55.0% | |
| GCACATGTAGAGGGCGGTTA+TGG | + | Chr2:28116019-28116038 | MsG0280008258.01.T01:CDS | 55.0% | |
| GGAGGACGAAGCACATGTAG+AGG | + | Chr2:28116009-28116028 | MsG0280008258.01.T01:CDS | 55.0% | |
| ACTCTGCCGGGACTCATCGA+AGG | - | Chr2:28115958-28115977 | None:intergenic | 60.0% | |
| TTCGGCCACTGCAACTCTGC+CGG | - | Chr2:28115971-28115990 | None:intergenic | 60.0% | |
| GAGTCCCGGCAGAGTTGCAG+TGG | + | Chr2:28115963-28115982 | MsG0280008258.01.T01:CDS | 65.0% | |
| TCGGCCACTGCAACTCTGCC+GGG | - | Chr2:28115970-28115989 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 28115562 | 28116116 | 28115562 | ID=MsG0280008258.01;Name=MsG0280008258.01 |
| Chr2 | mRNA | 28115562 | 28116116 | 28115562 | ID=MsG0280008258.01.T01;Parent=MsG0280008258.01;Name=MsG0280008258.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|184 |
| Chr2 | exon | 28115562 | 28116116 | 28115562 | ID=MsG0280008258.01.T01:exon:6148;Parent=MsG0280008258.01.T01 |
| Chr2 | CDS | 28115562 | 28116116 | 28115562 | ID=MsG0280008258.01.T01:cds;Parent=MsG0280008258.01.T01 |
| Gene Sequence |
| Protein sequence |
