AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010534.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010534.01.T01 MTR_2g086950 89.924 397 23 1 13 409 89 468 0.0 632
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010534.01.T01 AT5G22320 40.594 404 190 12 13 407 89 451 1.06e-66 219
MsG0280010534.01.T01 AT5G22320 38.366 404 183 13 13 407 89 435 3.91e-56 191

Find 110 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTGTGCATTATTTCTTC+TGG 0.177535 2:-71051894 None:intergenic
AGAAATTCATTTGAAGTAAT+TGG 0.187992 2:-71053239 None:intergenic
CCCATTCCAATTTCTGTTAC+TGG 0.188491 2:-71052858 None:intergenic
CATTGCATTTCTCCTTGTTC+AGG 0.189521 2:+71052979 MsG0280010534.01.T01:three_prime_UTR
AGACTCCACTTTATGCATTT+TGG 0.207816 2:-71052826 None:intergenic
TGCAAAATGGTCAGATATAA+AGG 0.253542 2:+71051953 MsG0280010534.01.T01:CDS
TTCCTGGTATCTTTATCTAT+TGG 0.262208 2:-71052273 None:intergenic
TCATCTCCAGTAACAGAAAT+TGG 0.264149 2:+71052852 MsG0280010534.01.T01:CDS
TACACTATTGTGCTAACTTT+TGG 0.280473 2:+71051858 MsG0280010534.01.T01:CDS
TCTTGCTCACAATGATATTA+AGG 0.282764 2:+71051712 MsG0280010534.01.T01:CDS
AAAGCTTCGGAATTTAGATT+TGG 0.286348 2:+71051917 MsG0280010534.01.T01:CDS
AAGAAAATTTAAACTCCCTT+TGG 0.331012 2:-71053347 None:intergenic
AAGCTTCGGAATTTAGATTT+GGG 0.337577 2:+71051918 MsG0280010534.01.T01:CDS
ACAAAATACTCAATAAATAA+TGG 0.340042 2:-71053214 None:intergenic
CAGATGTATATCATTACATT+TGG 0.345180 2:-71053394 None:intergenic
CAAGTGACCTTTCTCATTCC+TGG 0.348358 2:-71052289 None:intergenic
ACAGGTCATAACAAGGATAA+TGG 0.368572 2:+71052462 MsG0280010534.01.T01:CDS
ACTTTCTCATTTGTAGCAAC+AGG 0.381222 2:-71052109 None:intergenic
GGTATCTTTATCTATTGGTT+TGG 0.382169 2:-71052268 None:intergenic
TAAATTGAGATTTCTCAGTT+TGG 0.382838 2:-71052077 None:intergenic
ATAGACCCCACCTTCCTTCT+CGG 0.391386 2:-71052427 None:intergenic
AATTGTGCATTATTTCTTCT+GGG 0.394886 2:-71051893 None:intergenic
AGAGGAGACATAAAACTTAA+TGG 0.396284 2:-71053269 None:intergenic
ATTGCATTTCTCCTTGTTCA+GGG 0.397484 2:+71052980 MsG0280010534.01.T01:three_prime_UTR
GTTGATTTATTCTTCGTGTC+AGG 0.403540 2:-71052522 None:intergenic
CATCTGATCAAGTTTGCAAA+TGG 0.405995 2:-71050911 None:intergenic
CCTTTCACATTGTCAGATTG+AGG 0.407813 2:+71051643 MsG0280010534.01.T01:CDS
TCAGAGCCTCGCCAATCTTA+CGG 0.436737 2:-71051497 None:intergenic
GCGTTGAATACTTGTAGCTT+TGG 0.437925 2:-71052246 None:intergenic
ATCTTGAAGCTGCTGACAAA+AGG 0.439283 2:+71052367 MsG0280010534.01.T01:CDS
CCACCTGGGTCTAGCACAAA+TGG 0.447526 2:-71053104 None:intergenic
CTCTCGAGGAGAATGTTAAT+AGG 0.448873 2:+71052595 MsG0280010534.01.T01:CDS
CTTTCACATTGTCAGATTGA+GGG 0.449337 2:+71051644 MsG0280010534.01.T01:CDS
TTGGCACTCTTATGCTTGAC+AGG 0.455417 2:-71052807 None:intergenic
TACTCAATAAATAATGGAAA+AGG 0.460616 2:-71053208 None:intergenic
TGAATCGAACTCGGTACTCT+AGG 0.462484 2:+71053050 MsG0280010534.01.T01:three_prime_UTR
ATTTCAAATCCTTAGGCGCT+TGG 0.462547 2:-71052761 None:intergenic
TAATGCACAATTCAAAGCTT+CGG 0.462950 2:+71051904 MsG0280010534.01.T01:CDS
TTTCATAGTAGTCATGCAAA+AGG 0.463184 2:-71053299 None:intergenic
GGAAGGTGGGGTCTATGATA+AGG 0.467284 2:+71052434 MsG0280010534.01.T01:CDS
AATAATGGAAAAGGCAAGAA+AGG 0.468786 2:-71053199 None:intergenic
TGGCAAATGCAACAATATCT+TGG 0.480404 2:+71053423 MsG0280010534.01.T01:three_prime_UTR
CTCAATCTGACAATGTGAAA+GGG 0.482918 2:-71051642 None:intergenic
CTCACTAATTTCAAATCCTT+AGG 0.490267 2:-71052768 None:intergenic
ATGCCACCTTTGAGTATACA+TGG 0.492684 2:-71053449 None:intergenic
CATGCAAAAGGGGAGGGGAG+AGG 0.494113 2:-71053287 None:intergenic
TTCATAGTAGTCATGCAAAA+GGG 0.494527 2:-71053298 None:intergenic
AATGTCAGCAAATGAAGCTT+CGG 0.495172 2:-71052682 None:intergenic
GGAATAATGTGATTGCAAAA+TGG 0.498927 2:+71051940 MsG0280010534.01.T01:CDS
AATTGTTGTTACTTTCCAAA+GGG 0.502368 2:+71053332 MsG0280010534.01.T01:three_prime_UTR
TTCACATATTAGCCTCCACC+TGG 0.502925 2:-71053119 None:intergenic
GAGTGCCAAAATGCATAAAG+TGG 0.507006 2:+71052821 MsG0280010534.01.T01:CDS
GTCTATGATAAGGAAAATAC+AGG 0.507147 2:+71052444 MsG0280010534.01.T01:CDS
AAAGGACGACGGCAAGCCCT+CGG 0.507847 2:+71052560 MsG0280010534.01.T01:CDS
GGCAAACATTACCTTTGTGA+TGG 0.510475 2:-71051532 None:intergenic
CCAGTAACAGAAATTGGAAT+GGG 0.514929 2:+71052858 MsG0280010534.01.T01:CDS
GCTACAGAACCAAGCGCCTA+AGG 0.515364 2:+71052752 MsG0280010534.01.T01:CDS
GATCAGGAAAATCTGAATCA+TGG 0.515970 2:+71052717 MsG0280010534.01.T01:CDS
ATGATATACATCTGAACCAC+TGG 0.522003 2:+71053403 MsG0280010534.01.T01:three_prime_UTR
CAGTTCAACACAAAACTTGA+GGG 0.525455 2:-71051678 None:intergenic
CTGAGAAATCTCAATTTACA+AGG 0.527846 2:+71052082 MsG0280010534.01.T01:CDS
GAATGGGAGGTTCATCAACA+TGG 0.536187 2:+71052874 MsG0280010534.01.T01:CDS
CCTCAATCTGACAATGTGAA+AGG 0.540601 2:-71051643 None:intergenic
ATGAAAGAATTGAATACTCT+CGG 0.542671 2:+71050931 MsG0280010534.01.T01:CDS
GTGAACCAGGTACTAAATGC+AGG 0.545787 2:+71050616 MsG0280010534.01.T01:CDS
GATTGGCGAGGCTCTGAAGA+AGG 0.550173 2:+71051503 MsG0280010534.01.T01:CDS
TCCAGTAACAGAAATTGGAA+TGG 0.551066 2:+71052857 MsG0280010534.01.T01:CDS
AAATGAGAAAGTTACAAGAA+AGG 0.556080 2:+71052120 MsG0280010534.01.T01:CDS
GTACCGAGTTCGATTCATAC+TGG 0.557570 2:-71053044 None:intergenic
GAATCGAACTCGGTACTCTA+GGG 0.559753 2:+71053051 MsG0280010534.01.T01:three_prime_UTR
AAGAGACAAACATCACAAGC+AGG 0.566680 2:-71053161 None:intergenic
GTAGTCATGCAAAAGGGGAG+GGG 0.566771 2:-71053292 None:intergenic
TGAGTCAGTAAGAAAACAGT+AGG 0.568093 2:-71052942 None:intergenic
AGTAGTCATGCAAAAGGGGA+GGG 0.568334 2:-71053293 None:intergenic
AAAGATACCAGGAATGAGAA+AGG 0.571389 2:+71052282 MsG0280010534.01.T01:CDS
AGCTTCGGAATTTAGATTTG+GGG 0.572795 2:+71051919 MsG0280010534.01.T01:CDS
AAATTGTTGTTACTTTCCAA+AGG 0.579314 2:+71053331 MsG0280010534.01.T01:three_prime_UTR
AACTGTTGATGTATCCGAGA+AGG 0.580615 2:+71052413 MsG0280010534.01.T01:CDS
GATGTATCCGAGAAGGAAGG+TGG 0.583676 2:+71052420 MsG0280010534.01.T01:CDS
AACTGTGCTCACCCTGAACA+AGG 0.591893 2:-71052991 None:intergenic
TGGCATCCATGTATACTCAA+AGG 0.601824 2:+71053443 MsG0280010534.01.T01:three_prime_UTR
GATATTGTTGCATTTGCCAG+TGG 0.603591 2:-71053419 None:intergenic
AGATCATGTCGATCAAAATG+AGG 0.604619 2:+71052338 MsG0280010534.01.T01:CDS
CACCATTCAAAATCAGTGCA+CGG 0.606464 2:-71050687 None:intergenic
GCCTCGCCAATCTTACGGAT+AGG 0.606743 2:-71051492 None:intergenic
TCATAGTAGTCATGCAAAAG+GGG 0.614238 2:-71053297 None:intergenic
AAAGAAGAATCGAAAGAACA+AGG 0.618334 2:+71052629 MsG0280010534.01.T01:CDS
TTTGTGCTAGACCCAGGTGG+AGG 0.618385 2:+71053107 MsG0280010534.01.T01:three_prime_UTR
ATGTATCCGAGAAGGAAGGT+GGG 0.618544 2:+71052421 MsG0280010534.01.T01:CDS
TAGTAGTCATGCAAAAGGGG+AGG 0.622420 2:-71053294 None:intergenic
TTTATACGATCATGTGAACC+AGG 0.623831 2:+71050603 MsG0280010534.01.T01:CDS
AAACCATTTGTGCTAGACCC+AGG 0.623902 2:+71053101 MsG0280010534.01.T01:three_prime_UTR
AATGGGAGGTTCATCAACAT+GGG 0.631794 2:+71052875 MsG0280010534.01.T01:CDS
GGAAAATACAGGTCATAACA+AGG 0.632189 2:+71052455 MsG0280010534.01.T01:CDS
TCAGTTCAACACAAAACTTG+AGG 0.639929 2:-71051679 None:intergenic
TGACCAGTATGAATCGAACT+CGG 0.640275 2:+71053041 MsG0280010534.01.T01:three_prime_UTR
GTTGATGTATCCGAGAAGGA+AGG 0.641292 2:+71052417 MsG0280010534.01.T01:CDS
GTAACAGAAATTGGAATGGG+AGG 0.647412 2:+71052861 MsG0280010534.01.T01:CDS
AACCAATAGATAAAGATACC+AGG 0.653470 2:+71052271 MsG0280010534.01.T01:CDS
AGAGACAAACATCACAAGCA+GGG 0.656053 2:-71053160 None:intergenic
TCCTATCCGTAAGATTGGCG+AGG 0.659591 2:+71051491 MsG0280010534.01.T01:CDS
CATCCATGTATACTCAAAGG+TGG 0.664511 2:+71053446 MsG0280010534.01.T01:three_prime_UTR
TGTATCCGAGAAGGAAGGTG+GGG 0.665884 2:+71052422 MsG0280010534.01.T01:CDS
ATTCAAAATCAGTGCACGGA+GGG 0.667546 2:-71050683 None:intergenic
TCACATATTAGCCTCCACCT+GGG 0.672930 2:-71053118 None:intergenic
GAAGGTGAAATCCATCACAA+AGG 0.679265 2:+71051521 MsG0280010534.01.T01:CDS
CCATTTGTGCTAGACCCAGG+TGG 0.702098 2:+71053104 MsG0280010534.01.T01:three_prime_UTR
ATGGGAGGTTCATCAACATG+GGG 0.706743 2:+71052876 MsG0280010534.01.T01:CDS
CAGGAAAATCTGAATCATGG+TGG 0.716042 2:+71052720 MsG0280010534.01.T01:CDS
CATTCAAAATCAGTGCACGG+AGG 0.728165 2:-71050684 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAACTTAAATCTA+TGG + Chr2:71050639-71050658 MsG0280010534.01.T01:CDS 10.0%
!! ACAAAATACTCAATAAATAA+TGG - Chr2:71053217-71053236 None:intergenic 15.0%
!! TATTCAAAAATTATTTGAGC+TGG - Chr2:71051442-71051461 None:intergenic 20.0%
!!! AGAAATTCATTTGAAGTAAT+TGG - Chr2:71053242-71053261 None:intergenic 20.0%
!!! TACTCAATAAATAATGGAAA+AGG - Chr2:71053211-71053230 None:intergenic 20.0%
!!! TTTGAAATGCAAACTATTTT+TGG + Chr2:71051392-71051411 MsG0280010534.01.T01:intron 20.0%
! AAATAACTAATGAGCACTAA+AGG - Chr2:71051574-71051593 None:intergenic 25.0%
! AAATGAGAAAGTTACAAGAA+AGG + Chr2:71052120-71052139 MsG0280010534.01.T01:CDS 25.0%
! AACGAAGAAAAAGCTAAAAA+AGG + Chr2:71052542-71052561 MsG0280010534.01.T01:CDS 25.0%
! AAGAAAATTTAAACTCCCTT+TGG - Chr2:71053350-71053369 None:intergenic 25.0%
! ATGAAAGAATTGAATACTCT+CGG + Chr2:71050931-71050950 MsG0280010534.01.T01:CDS 25.0%
! ATTCAAACTGTCTTGATATA+AGG + Chr2:71051259-71051278 MsG0280010534.01.T01:intron 25.0%
! CAGATGTATATCATTACATT+TGG - Chr2:71053397-71053416 None:intergenic 25.0%
! TAAATTGAGATTTCTCAGTT+TGG - Chr2:71052080-71052099 None:intergenic 25.0%
!! AATTGTGCATTATTTCTTCT+GGG - Chr2:71051896-71051915 None:intergenic 25.0%
!! ATTACTCATAATTCCTCTTT+TGG + Chr2:71051609-71051628 MsG0280010534.01.T01:intron 25.0%
!! ATTTTGAACCAAAAAAAGCA+AGG - Chr2:71052151-71052170 None:intergenic 25.0%
!! TTTTGAACCAAAAAAAGCAA+GGG - Chr2:71052150-71052169 None:intergenic 25.0%
!!! AAATTGTTGTTACTTTCCAA+AGG + Chr2:71053331-71053350 MsG0280010534.01.T01:three_prime_UTR 25.0%
!!! AATTGTTGTTACTTTCCAAA+GGG + Chr2:71053332-71053351 MsG0280010534.01.T01:three_prime_UTR 25.0%
!!! AGTTTCCTTTCTTTTTTTAC+TGG - Chr2:71052397-71052416 None:intergenic 25.0%
!!! TTTTTGATTCTACATGCTTT+TGG + Chr2:71051759-71051778 MsG0280010534.01.T01:intron 25.0%
AAAAAGAAAGACAAACTCAC+AGG + Chr2:71052489-71052508 MsG0280010534.01.T01:CDS 30.0%
AAAAAGCTAAAAAAGGACGA+CGG + Chr2:71052549-71052568 MsG0280010534.01.T01:CDS 30.0%
AAAAATCCTATCCGTAAGAT+TGG + Chr2:71051486-71051505 MsG0280010534.01.T01:CDS 30.0%
AAAGAAGAATCGAAAGAACA+AGG + Chr2:71052629-71052648 MsG0280010534.01.T01:CDS 30.0%
AAAGCTTCGGAATTTAGATT+TGG + Chr2:71051917-71051936 MsG0280010534.01.T01:CDS 30.0%
AACCAATAGATAAAGATACC+AGG + Chr2:71052271-71052290 MsG0280010534.01.T01:CDS 30.0%
AAGCTTCGGAATTTAGATTT+GGG + Chr2:71051918-71051937 MsG0280010534.01.T01:CDS 30.0%
AATCTGTAACATCATACACA+TGG - Chr2:71052214-71052233 None:intergenic 30.0%
ACCATCAGCAAGATATTATT+TGG - Chr2:71051418-71051437 None:intergenic 30.0%
AGAGGAGACATAAAACTTAA+TGG - Chr2:71053272-71053291 None:intergenic 30.0%
AGATATAAAGGTTTGTCATC+TGG + Chr2:71051965-71051984 MsG0280010534.01.T01:intron 30.0%
ATCATACACATGGTAGTTTA+AGG - Chr2:71052204-71052223 None:intergenic 30.0%
CTCACTAATTTCAAATCCTT+AGG - Chr2:71052771-71052790 None:intergenic 30.0%
CTGAGAAATCTCAATTTACA+AGG + Chr2:71052082-71052101 MsG0280010534.01.T01:CDS 30.0%
GAAGTCCAGTAAAAAAAGAA+AGG + Chr2:71052389-71052408 MsG0280010534.01.T01:CDS 30.0%
GGAATAATGTGATTGCAAAA+TGG + Chr2:71051940-71051959 MsG0280010534.01.T01:CDS 30.0%
GTCTATGATAAGGAAAATAC+AGG + Chr2:71052444-71052463 MsG0280010534.01.T01:CDS 30.0%
TAATGCACAATTCAAAGCTT+CGG + Chr2:71051904-71051923 MsG0280010534.01.T01:CDS 30.0%
TCTTGCTCACAATGATATTA+AGG + Chr2:71051712-71051731 MsG0280010534.01.T01:CDS 30.0%
TGAAAAATCATGAGCATCAT+CGG - Chr2:71052316-71052335 None:intergenic 30.0%
TGCAAAATGGTCAGATATAA+AGG + Chr2:71051953-71051972 MsG0280010534.01.T01:CDS 30.0%
TTCAACTTTCAAAATAGACG+AGG - Chr2:71050739-71050758 None:intergenic 30.0%
TTCATAGTAGTCATGCAAAA+GGG - Chr2:71053301-71053320 None:intergenic 30.0%
TTCCTGGTATCTTTATCTAT+TGG - Chr2:71052276-71052295 None:intergenic 30.0%
TTGCATTTCAAACATACAAC+AGG - Chr2:71051384-71051403 None:intergenic 30.0%
TTTCATAGTAGTCATGCAAA+AGG - Chr2:71053302-71053321 None:intergenic 30.0%
! AAACAAGAGATTTTGTAAGC+TGG + Chr2:71051170-71051189 MsG0280010534.01.T01:intron 30.0%
! AATAATGGAAAAGGCAAGAA+AGG - Chr2:71053202-71053221 None:intergenic 30.0%
! CATTTTTAACATCAAGGATC+AGG + Chr2:71052701-71052720 MsG0280010534.01.T01:CDS 30.0%
! GAATTGTGCATTATTTCTTC+TGG - Chr2:71051897-71051916 None:intergenic 30.0%
! GGTATCTTTATCTATTGGTT+TGG - Chr2:71052271-71052290 None:intergenic 30.0%
! TACACTATTGTGCTAACTTT+TGG + Chr2:71051858-71051877 MsG0280010534.01.T01:CDS 30.0%
! TGCTGACATTTTTAACATCA+AGG + Chr2:71052695-71052714 MsG0280010534.01.T01:CDS 30.0%
!!! TGCTTTTGGAATGTTTAATG+TGG + Chr2:71051773-71051792 MsG0280010534.01.T01:intron 30.0%
AAAGATACCAGGAATGAGAA+AGG + Chr2:71052282-71052301 MsG0280010534.01.T01:CDS 35.0%
AATGTCAGCAAATGAAGCTT+CGG - Chr2:71052685-71052704 None:intergenic 35.0%
ACAGGTCATAACAAGGATAA+TGG + Chr2:71052462-71052481 MsG0280010534.01.T01:CDS 35.0%
ACAGTTGCAATACAAGATGA+AGG - Chr2:71052039-71052058 None:intergenic 35.0%
ACTTTCTCATTTGTAGCAAC+AGG - Chr2:71052112-71052131 None:intergenic 35.0%
AGACAACGATAAAACGTTGT+TGG - Chr2:71051998-71052017 None:intergenic 35.0%
AGATCATGTCGATCAAAATG+AGG + Chr2:71052338-71052357 MsG0280010534.01.T01:CDS 35.0%
AGCTTCGGAATTTAGATTTG+GGG + Chr2:71051919-71051938 MsG0280010534.01.T01:CDS 35.0%
ATGATATACATCTGAACCAC+TGG + Chr2:71053403-71053422 MsG0280010534.01.T01:three_prime_UTR 35.0%
ATTGCATTTCTCCTTGTTCA+GGG + Chr2:71052980-71052999 MsG0280010534.01.T01:three_prime_UTR 35.0%
CAGTTCAACACAAAACTTGA+GGG - Chr2:71051681-71051700 None:intergenic 35.0%
CATCTGATCAAGTTTGCAAA+TGG - Chr2:71050914-71050933 None:intergenic 35.0%
CCAGTAACAGAAATTGGAAT+GGG + Chr2:71052858-71052877 MsG0280010534.01.T01:CDS 35.0%
CTCAATCTGACAATGTGAAA+GGG - Chr2:71051645-71051664 None:intergenic 35.0%
CTCTTCTTATAAAACCCCTT+GGG - Chr2:71051298-71051317 None:intergenic 35.0%
CTTTCACATTGTCAGATTGA+GGG + Chr2:71051644-71051663 MsG0280010534.01.T01:CDS 35.0%
GATCAGGAAAATCTGAATCA+TGG + Chr2:71052717-71052736 MsG0280010534.01.T01:CDS 35.0%
GCCAAATAATATCTTGCTGA+TGG + Chr2:71051414-71051433 MsG0280010534.01.T01:intron 35.0%
GGAAAATACAGGTCATAACA+AGG + Chr2:71052455-71052474 MsG0280010534.01.T01:CDS 35.0%
TCAGTTCAACACAAAACTTG+AGG - Chr2:71051682-71051701 None:intergenic 35.0%
TCATAGTAGTCATGCAAAAG+GGG - Chr2:71053300-71053319 None:intergenic 35.0%
TCATCTCCAGTAACAGAAAT+TGG + Chr2:71052852-71052871 MsG0280010534.01.T01:CDS 35.0%
TCATCTTGTATTGCAACTGT+AGG + Chr2:71052039-71052058 MsG0280010534.01.T01:intron 35.0%
TCCAGTAACAGAAATTGGAA+TGG + Chr2:71052857-71052876 MsG0280010534.01.T01:CDS 35.0%
TCGCTAAAGTCTGAAAAAGA+TGG - Chr2:71051034-71051053 None:intergenic 35.0%
TGAGTCAGTAAGAAAACAGT+AGG - Chr2:71052945-71052964 None:intergenic 35.0%
TGGCAAATGCAACAATATCT+TGG + Chr2:71053423-71053442 MsG0280010534.01.T01:three_prime_UTR 35.0%
TTTATACGATCATGTGAACC+AGG + Chr2:71050603-71050622 MsG0280010534.01.T01:CDS 35.0%
! AGACTCCACTTTATGCATTT+TGG - Chr2:71052829-71052848 None:intergenic 35.0%
! CAATACAAGATGAAGGTACA+TGG - Chr2:71052032-71052051 None:intergenic 35.0%
! GTTGATTTATTCTTCGTGTC+AGG - Chr2:71052525-71052544 None:intergenic 35.0%
! TTTTTCCTGCATTTAGTACC+TGG - Chr2:71050624-71050643 None:intergenic 35.0%
!! AGTCTGAAAAAGATGGACTA+TGG - Chr2:71051027-71051046 None:intergenic 35.0%
!! CAGTAGGTTTTTAAGCATAG+CGG - Chr2:71052929-71052948 None:intergenic 35.0%
AACTGTTGATGTATCCGAGA+AGG + Chr2:71052413-71052432 MsG0280010534.01.T01:CDS 40.0%
AAGAGACAAACATCACAAGC+AGG - Chr2:71053164-71053183 None:intergenic 40.0%
AATGGGAGGTTCATCAACAT+GGG + Chr2:71052875-71052894 MsG0280010534.01.T01:CDS 40.0%
ACTTTCAAAATAGACGAGGC+TGG - Chr2:71050735-71050754 None:intergenic 40.0%
AGAGACAAACATCACAAGCA+GGG - Chr2:71053163-71053182 None:intergenic 40.0%
AGGATCAACAGTATTTCCCA+AGG + Chr2:71051279-71051298 MsG0280010534.01.T01:intron 40.0%
ATCTTGAAGCTGCTGACAAA+AGG + Chr2:71052367-71052386 MsG0280010534.01.T01:CDS 40.0%
ATTCAAAATCAGTGCACGGA+GGG - Chr2:71050686-71050705 None:intergenic 40.0%
ATTTCAAATCCTTAGGCGCT+TGG - Chr2:71052764-71052783 None:intergenic 40.0%
CACCATTCAAAATCAGTGCA+CGG - Chr2:71050690-71050709 None:intergenic 40.0%
CAGGAAAATCTGAATCATGG+TGG + Chr2:71052720-71052739 MsG0280010534.01.T01:CDS 40.0%
CATTGCATTTCTCCTTGTTC+AGG + Chr2:71052979-71052998 MsG0280010534.01.T01:three_prime_UTR 40.0%
CCCATTCCAATTTCTGTTAC+TGG - Chr2:71052861-71052880 None:intergenic 40.0%
CCTCAATCTGACAATGTGAA+AGG - Chr2:71051646-71051665 None:intergenic 40.0%
CCTCTTCTTATAAAACCCCT+TGG - Chr2:71051299-71051318 None:intergenic 40.0%
CCTTTCACATTGTCAGATTG+AGG + Chr2:71051643-71051662 MsG0280010534.01.T01:CDS 40.0%
CTCTCGAGGAGAATGTTAAT+AGG + Chr2:71052595-71052614 MsG0280010534.01.T01:CDS 40.0%
GAAGGTGAAATCCATCACAA+AGG + Chr2:71051521-71051540 MsG0280010534.01.T01:CDS 40.0%
GAGTGCCAAAATGCATAAAG+TGG + Chr2:71052821-71052840 MsG0280010534.01.T01:CDS 40.0%
GATATTGTTGCATTTGCCAG+TGG - Chr2:71053422-71053441 None:intergenic 40.0%
GATCAACAGTATTTCCCAAG+GGG + Chr2:71051281-71051300 MsG0280010534.01.T01:intron 40.0%
GCGTTGAATACTTGTAGCTT+TGG - Chr2:71052249-71052268 None:intergenic 40.0%
GGATCAACAGTATTTCCCAA+GGG + Chr2:71051280-71051299 MsG0280010534.01.T01:intron 40.0%
GGCAAACATTACCTTTGTGA+TGG - Chr2:71051535-71051554 None:intergenic 40.0%
GTAACAGAAATTGGAATGGG+AGG + Chr2:71052861-71052880 MsG0280010534.01.T01:CDS 40.0%
TAAAGGAGCTCAACAGCTTA+GGG - Chr2:71051557-71051576 None:intergenic 40.0%
TGACCAGTATGAATCGAACT+CGG + Chr2:71053041-71053060 MsG0280010534.01.T01:three_prime_UTR 40.0%
TGCTGTGCTTTCATCACAAT+AGG + Chr2:71051069-71051088 MsG0280010534.01.T01:intron 40.0%
TGGCATCCATGTATACTCAA+AGG + Chr2:71053443-71053462 MsG0280010534.01.T01:three_prime_UTR 40.0%
!! AGGGGTTTTATAAGAAGAGG+AGG + Chr2:71051299-71051318 MsG0280010534.01.T01:intron 40.0%
!! CCAAGGGGTTTTATAAGAAG+AGG + Chr2:71051296-71051315 MsG0280010534.01.T01:intron 40.0%
!!! AGGTGATCCCTTGCTTTTTT+TGG + Chr2:71052140-71052159 MsG0280010534.01.T01:intron 40.0%
AAACCATTTGTGCTAGACCC+AGG + Chr2:71053101-71053120 MsG0280010534.01.T01:three_prime_UTR 45.0%
AAAGGAGCTCAACAGCTTAG+GGG - Chr2:71051556-71051575 None:intergenic 45.0%
AGTAGTCATGCAAAAGGGGA+GGG - Chr2:71053296-71053315 None:intergenic 45.0%
ATGGGAGGTTCATCAACATG+GGG + Chr2:71052876-71052895 MsG0280010534.01.T01:CDS 45.0%
ATGTATCCGAGAAGGAAGGT+GGG + Chr2:71052421-71052440 MsG0280010534.01.T01:CDS 45.0%
CAAGTGACCTTTCTCATTCC+TGG - Chr2:71052292-71052311 None:intergenic 45.0%
CATTCAAAATCAGTGCACGG+AGG - Chr2:71050687-71050706 None:intergenic 45.0%
CTAAAGGAGCTCAACAGCTT+AGG - Chr2:71051558-71051577 None:intergenic 45.0%
CTGCTAGCGCAGAAAATCTT+AGG - Chr2:71050992-71051011 None:intergenic 45.0%
GAATCGAACTCGGTACTCTA+GGG + Chr2:71053051-71053070 MsG0280010534.01.T01:three_prime_UTR 45.0%
GAATGGGAGGTTCATCAACA+TGG + Chr2:71052874-71052893 MsG0280010534.01.T01:CDS 45.0%
GACAATGTGAAAGGGAAAGC+TGG - Chr2:71051637-71051656 None:intergenic 45.0%
GTACCGAGTTCGATTCATAC+TGG - Chr2:71053047-71053066 None:intergenic 45.0%
GTGAACCAGGTACTAAATGC+AGG + Chr2:71050616-71050635 MsG0280010534.01.T01:CDS 45.0%
GTTGATGTATCCGAGAAGGA+AGG + Chr2:71052417-71052436 MsG0280010534.01.T01:CDS 45.0%
TAGTAGTCATGCAAAAGGGG+AGG - Chr2:71053297-71053316 None:intergenic 45.0%
TGAATCGAACTCGGTACTCT+AGG + Chr2:71053050-71053069 MsG0280010534.01.T01:three_prime_UTR 45.0%
TTGGCACTCTTATGCTTGAC+AGG - Chr2:71052810-71052829 None:intergenic 45.0%
! CTAAGATTTTCTGCGCTAGC+AGG + Chr2:71050990-71051009 MsG0280010534.01.T01:intron 45.0%
! TCACATATTAGCCTCCACCT+GGG - Chr2:71053121-71053140 None:intergenic 45.0%
! TTCACATATTAGCCTCCACC+TGG - Chr2:71053122-71053141 None:intergenic 45.0%
!!! CTCCGTGCACTGATTTTGAA+TGG + Chr2:71050685-71050704 MsG0280010534.01.T01:CDS 45.0%
AACTGTGCTCACCCTGAACA+AGG - Chr2:71052994-71053013 None:intergenic 50.0%
ATAGACCCCACCTTCCTTCT+CGG - Chr2:71052430-71052449 None:intergenic 50.0%
GATGTATCCGAGAAGGAAGG+TGG + Chr2:71052420-71052439 MsG0280010534.01.T01:CDS 50.0%
GGAAGGTGGGGTCTATGATA+AGG + Chr2:71052434-71052453 MsG0280010534.01.T01:CDS 50.0%
GGGAAAGCTGGAACCAAAAG+AGG - Chr2:71051625-71051644 None:intergenic 50.0%
GTAGTCATGCAAAAGGGGAG+GGG - Chr2:71053295-71053314 None:intergenic 50.0%
TCAGAGCCTCGCCAATCTTA+CGG - Chr2:71051500-71051519 None:intergenic 50.0%
TCCTATCCGTAAGATTGGCG+AGG + Chr2:71051491-71051510 MsG0280010534.01.T01:CDS 50.0%
TGTATCCGAGAAGGAAGGTG+GGG + Chr2:71052422-71052441 MsG0280010534.01.T01:CDS 50.0%
!!! AGAGCCAACGCTTTTTCCGA+GGG - Chr2:71052579-71052598 None:intergenic 50.0%
CCACCTGGGTCTAGCACAAA+TGG - Chr2:71053107-71053126 None:intergenic 55.0%
CCATTTGTGCTAGACCCAGG+TGG + Chr2:71053104-71053123 MsG0280010534.01.T01:three_prime_UTR 55.0%
GCCTCGCCAATCTTACGGAT+AGG - Chr2:71051495-71051514 None:intergenic 55.0%
GCTACAGAACCAAGCGCCTA+AGG + Chr2:71052752-71052771 MsG0280010534.01.T01:CDS 55.0%
TTTGTGCTAGACCCAGGTGG+AGG + Chr2:71053107-71053126 MsG0280010534.01.T01:three_prime_UTR 55.0%
! CAAGCCCTCGGAAAAAGCGT+TGG + Chr2:71052572-71052591 MsG0280010534.01.T01:CDS 55.0%
!! GATTGGCGAGGCTCTGAAGA+AGG + Chr2:71051503-71051522 MsG0280010534.01.T01:CDS 55.0%
!! GGAAAAAGCGTTGGCTCTCG+AGG + Chr2:71052581-71052600 MsG0280010534.01.T01:CDS 55.0%
!!! GAGAGCCAACGCTTTTTCCG+AGG - Chr2:71052580-71052599 None:intergenic 55.0%
AAAGGACGACGGCAAGCCCT+CGG + Chr2:71052560-71052579 MsG0280010534.01.T01:CDS 60.0%
CATGCAAAAGGGGAGGGGAG+AGG - Chr2:71053290-71053309 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 71050589 71053465 71050589 ID=MsG0280010534.01;Name=MsG0280010534.01
Chr2 mRNA 71050589 71053465 71050589 ID=MsG0280010534.01.T01;Parent=MsG0280010534.01;Name=MsG0280010534.01.T01;_AED=0.45;_eAED=0.47;_QI=0|0.83|0.71|1|1|1|7|560|409
Chr2 exon 71050589 71050706 71050589 ID=MsG0280010534.01.T01:exon:10392;Parent=MsG0280010534.01.T01
Chr2 exon 71050896 71050952 71050896 ID=MsG0280010534.01.T01:exon:10393;Parent=MsG0280010534.01.T01
Chr2 exon 71051478 71051542 71051478 ID=MsG0280010534.01.T01:exon:10394;Parent=MsG0280010534.01.T01
Chr2 exon 71051638 71051733 71051638 ID=MsG0280010534.01.T01:exon:10395;Parent=MsG0280010534.01.T01
Chr2 exon 71051837 71051974 71051837 ID=MsG0280010534.01.T01:exon:10396;Parent=MsG0280010534.01.T01
Chr2 exon 71052061 71052141 71052061 ID=MsG0280010534.01.T01:exon:10397;Parent=MsG0280010534.01.T01
Chr2 exon 71052231 71053465 71052231 ID=MsG0280010534.01.T01:exon:10398;Parent=MsG0280010534.01.T01
Chr2 CDS 71050589 71050706 71050589 ID=MsG0280010534.01.T01:cds;Parent=MsG0280010534.01.T01
Chr2 CDS 71050896 71050952 71050896 ID=MsG0280010534.01.T01:cds;Parent=MsG0280010534.01.T01
Chr2 CDS 71051478 71051542 71051478 ID=MsG0280010534.01.T01:cds;Parent=MsG0280010534.01.T01
Chr2 CDS 71051638 71051733 71051638 ID=MsG0280010534.01.T01:cds;Parent=MsG0280010534.01.T01
Chr2 CDS 71051837 71051974 71051837 ID=MsG0280010534.01.T01:cds;Parent=MsG0280010534.01.T01
Chr2 CDS 71052061 71052141 71052061 ID=MsG0280010534.01.T01:cds;Parent=MsG0280010534.01.T01
Chr2 CDS 71052231 71052905 71052231 ID=MsG0280010534.01.T01:cds;Parent=MsG0280010534.01.T01
Chr2 three_prime_UTR 71052906 71053465 71052906 ID=MsG0280010534.01.T01:three_prime_utr;Parent=MsG0280010534.01.T01
Gene Sequence

>MsG0280010534.01.T01

ATGCAAGAGTCACTTTTATACGATCATGTGAACCAGGTACTAAATGCAGGAAAAAATAAACTTAAATCTATGGATGAGATTGCGTCACTTAGTACCCTCCGTGCACTGATTTTGAATGACAATGAGATTATCTCCATTTGCAAACTTGATCAGATGAAAGAATTGAATACTCTCGTTTTATCTAAAAATCCTATCCGTAAGATTGGCGAGGCTCTGAAGAAGGTGAAATCCATCACAAAGCTTTCCCTTTCACATTGTCAGATTGAGGGTATTGACACTTCCCTCAAGTTTTGTGTTGAACTGACAGAGCTTCGTCTTGCTCACAATGATATTAAGAATATTTTGCTTTATATTGTGTACACTATTGTGCTAACTTTTGGATTTCAGTCTCTCCCAGAAGAAATAATGCACAATTCAAAGCTTCGGAATTTAGATTTGGGGAATAATGTGATTGCAAAATGGTCAGATATAAAGGTGCTCAAATCACTCACCAAACTGAGAAATCTCAATTTACAAGGAAATCCTGTTGCTACAAATGAGAAAGTTACAAGAAAGATTAAGAATGCATTGCCAAAGCTACAAGTATTCAACGCCAAACCAATAGATAAAGATACCAGGAATGAGAAAGGTCACTTGACCGATGATGCTCATGATTTTTCATTAGATCATGTCGATCAAAATGAGGATGATCATCTTGAAGCTGCTGACAAAAGGAAGTCCAGTAAAAAAAGAAAGGAAACTGTTGATGTATCCGAGAAGGAAGGTGGGGTCTATGATAAGGAAAATACAGGTCATAACAAGGATAATGGAGATAAAAAGAAAGACAAACTCACAGGTACTGTTGATCCTGACACGAAGAATAAATCAACGAAGAAAAAGCTAAAAAAGGACGACGGCAAGCCCTCGGAAAAAGCGTTGGCTCTCGAGGAGAATGTTAATAGGACTGAAAAGAAAAAGAAGAATCGAAAGAACAAGGAACAAAGTGAATTTGATATTATTGATGATGCCGAAGCTTCATTTGCTGACATTTTTAACATCAAGGATCAGGAAAATCTGAATCATGGTGGTGAAATGAAGCTACAGAACCAAGCGCCTAAGGATTTGAAATTAGTGAGCAGCATAGAGACTTCACCTGTCAAGCATAAGAGTGCCAAAATGCATAAAGTGGAGTCTCTATCATCTCCAGTAACAGAAATTGGAATGGGAGGTTCATCAACATGGGGCGATTAG
Protein sequence

>MsG0280010534.01.T01

MQESLLYDHVNQVLNAGKNKLKSMDEIASLSTLRALILNDNEIISICKLDQMKELNTLVLSKNPIRKIGEALKKVKSITKLSLSHCQIEGIDTSLKFCVELTELRLAHNDIKNILLYIVYTIVLTFGFQSLPEEIMHNSKLRNLDLGNNVIAKWSDIKVLKSLTKLRNLNLQGNPVATNEKVTRKIKNALPKLQVFNAKPIDKDTRNEKGHLTDDAHDFSLDHVDQNEDDHLEAADKRKSSKKRKETVDVSEKEGGVYDKENTGHNKDNGDKKKDKLTGTVDPDTKNKSTKKKLKKDDGKPSEKALALEENVNRTEKKKKNRKNKEQSEFDIIDDAEASFADIFNIKDQENLNHGGEMKLQNQAPKDLKLVSSIETSPVKHKSAKMHKVESLSSPVTEIGMGGSSTWGD*