Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010984.01.T01 | XP_003597310.1 | 97.798 | 545 | 11 | 1 | 1 | 545 | 1 | 544 | 0 | 1091 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010984.01.T01 | MTR_2g096190 | 97.798 | 545 | 11 | 1 | 1 | 545 | 1 | 544 | 0.0 | 1091 |
| MsG0280010984.01.T01 | MTR_4g062100 | 77.963 | 540 | 113 | 4 | 7 | 545 | 12 | 546 | 0.0 | 827 |
| MsG0280010984.01.T01 | MTR_7g100390 | 63.636 | 528 | 184 | 4 | 18 | 545 | 1 | 520 | 0.0 | 654 |
| MsG0280010984.01.T01 | MTR_7g117620 | 57.303 | 534 | 205 | 6 | 16 | 545 | 32 | 546 | 0.0 | 584 |
| MsG0280010984.01.T01 | MTR_1g115575 | 46.390 | 554 | 253 | 8 | 15 | 545 | 61 | 593 | 3.09e-156 | 459 |
| MsG0280010984.01.T01 | MTR_1g032100 | 43.893 | 524 | 269 | 8 | 36 | 545 | 19 | 531 | 7.28e-144 | 425 |
| MsG0280010984.01.T01 | MTR_8g105260 | 44.106 | 492 | 255 | 8 | 62 | 545 | 59 | 538 | 2.72e-135 | 404 |
| MsG0280010984.01.T01 | MTR_8g105260 | 30.583 | 206 | 133 | 4 | 21 | 222 | 92 | 291 | 8.48e-14 | 74.3 |
| MsG0280010984.01.T01 | MTR_7g100430 | 44.624 | 558 | 262 | 9 | 10 | 545 | 50 | 582 | 8.45e-135 | 404 |
| MsG0280010984.01.T01 | MTR_2g101310 | 25.801 | 593 | 337 | 14 | 25 | 543 | 68 | 631 | 4.03e-44 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010984.01.T01 | AT1G07710 | 74.812 | 532 | 132 | 1 | 14 | 545 | 14 | 543 | 0.0 | 769 |
| MsG0280010984.01.T01 | AT1G07710 | 74.812 | 532 | 132 | 1 | 14 | 545 | 14 | 543 | 0.0 | 769 |
| MsG0280010984.01.T01 | AT5G60070 | 66.667 | 555 | 168 | 8 | 1 | 545 | 1 | 548 | 0.0 | 694 |
| MsG0280010984.01.T01 | AT5G02620 | 61.453 | 537 | 184 | 4 | 15 | 545 | 5 | 524 | 0.0 | 630 |
| MsG0280010984.01.T01 | AT5G02620 | 61.453 | 537 | 184 | 4 | 15 | 545 | 5 | 524 | 0.0 | 630 |
| MsG0280010984.01.T01 | AT5G02620 | 61.453 | 537 | 184 | 4 | 15 | 545 | 5 | 524 | 0.0 | 630 |
| MsG0280010984.01.T01 | AT5G02620 | 58.929 | 560 | 184 | 5 | 15 | 545 | 5 | 547 | 0.0 | 619 |
| MsG0280010984.01.T01 | AT5G02620 | 58.929 | 560 | 184 | 5 | 15 | 545 | 5 | 547 | 0.0 | 619 |
| MsG0280010984.01.T01 | AT2G31820 | 59.160 | 524 | 203 | 6 | 24 | 545 | 148 | 662 | 0.0 | 598 |
| MsG0280010984.01.T01 | AT1G05640 | 58.879 | 535 | 207 | 8 | 15 | 545 | 102 | 627 | 0.0 | 582 |
Find 112 sgRNAs with CRISPR-Local
Find 157 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGACGAAACAATTGAC+AGG | 0.537514 | 2:-77960453 | MsG0280010984.01.T01:CDS |
| AAAATTATAAATGGTGCTTC+TGG | 0.278164 | 2:+77958390 | None:intergenic |
| AAAGAGCTGTTTCTCCATCT+TGG | 0.374656 | 2:+77960313 | None:intergenic |
| AAAGCCCTTCTATTAGAGCA+TGG | 0.609187 | 2:-77958738 | MsG0280010984.01.T01:CDS |
| AAATATGCTTCTAGGACAAA+AGG | 0.471296 | 2:-77958838 | MsG0280010984.01.T01:CDS |
| AACACCATGCTCTAATAGAA+GGG | 0.558802 | 2:+77958734 | None:intergenic |
| AACCAGGAAAGCCAGAACTC+GGG | 0.603570 | 2:-77959120 | MsG0280010984.01.T01:intron |
| AACTACTTCCTGCTTCCAAT+AGG | 0.491463 | 2:+77959393 | None:intergenic |
| AACTATAATGGCCACAACTT+TGG | 0.328211 | 2:-77958142 | MsG0280010984.01.T01:CDS |
| AAGAGCCAGGAGTTGCAACA+AGG | 0.608094 | 2:-77959280 | MsG0280010984.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGACGAAACAATTGAC+AGG | - | Chr2:77958029-77958048 | MsG0280010984.01.T01:CDS | 30.0% | |
| AAGCAAAGCAAAGAAACAAA+TGG | - | Chr2:77960190-77960209 | MsG0280010984.01.T01:CDS | 30.0% | |
| ACACGAATGTTATCAAATAG+CGG | + | Chr2:77958708-77958727 | None:intergenic | 30.0% | |
| AGACATATTGAAGATCCTTA+TGG | - | Chr2:77958972-77958991 | MsG0280010984.01.T01:intron | 30.0% | |
| AGATCCTTCAACAATAAACA+TGG | - | Chr2:77959305-77959324 | MsG0280010984.01.T01:CDS | 30.0% | |
| ATAGACATATACTAGGTACT+AGG | - | Chr2:77959413-77959432 | MsG0280010984.01.T01:CDS | 30.0% | |
| ATCAACAAGATGAATACAGA+AGG | - | Chr2:77959921-77959940 | MsG0280010984.01.T01:intron | 30.0% | |
| ATCCAAACAATATTCCAGAA+GGG | - | Chr2:77960042-77960061 | MsG0280010984.01.T01:intron | 30.0% | |
| ATGGTTATATTGATGTCGTT+AGG | - | Chr2:77958207-77958226 | MsG0280010984.01.T01:CDS | 30.0% | |
| CAGATTGTAAATATGCTTCT+AGG | - | Chr2:77959636-77959655 | MsG0280010984.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 77957981 | 77960523 | 77957981 | ID=MsG0280010984.01;Name=MsG0280010984.01 |
| Chr2 | mRNA | 77957981 | 77960523 | 77957981 | ID=MsG0280010984.01.T01;Parent=MsG0280010984.01;Name=MsG0280010984.01.T01;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|1|1|3|0|545 |
| Chr2 | exon | 77960181 | 77960523 | 77960181 | ID=MsG0280010984.01.T01:exon:11582;Parent=MsG0280010984.01.T01 |
| Chr2 | exon | 77959121 | 77959530 | 77959121 | ID=MsG0280010984.01.T01:exon:11581;Parent=MsG0280010984.01.T01 |
| Chr2 | exon | 77957981 | 77958865 | 77957981 | ID=MsG0280010984.01.T01:exon:11580;Parent=MsG0280010984.01.T01 |
| Chr2 | CDS | 77960181 | 77960523 | 77960181 | ID=MsG0280010984.01.T01:cds;Parent=MsG0280010984.01.T01 |
| Chr2 | CDS | 77959121 | 77959530 | 77959121 | ID=MsG0280010984.01.T01:cds;Parent=MsG0280010984.01.T01 |
| Chr2 | CDS | 77957981 | 77958865 | 77957981 | ID=MsG0280010984.01.T01:cds;Parent=MsG0280010984.01.T01 |
| Gene Sequence |
>MsG0280010984.01.T01 ATGGGTGAATCCAGCATGGCTACACCAATGGTGCACACACCTAGGAAAAAAATGACGAAACAATTGACAGGAAAAAGGGATGATACACCTTTGCATTCTGCAGCAAGAGCAGGGAATTTAGATGTTGTGAGGGATATTCTTAATGATGCTCAAGAGGATGAATTGCTTGAATTATTGGCTAGGCAGAACCAAGATGGAGAAACAGCTCTTTATGTTGCTGCTGAGTATGGTTATATTGATGTCGTTAGGGGAATGATTCAATATTATGATCTTGCTGGTGCTGGAATTAAAGCAAGAAACGGTTTTGATGCATTTCATATTGCTGCTAAACAAGGGGATATAGACATATTGAAGATCCTTATGGAGGTTCATCCTGAATTGTCAATGACAGTGGATCCATCAAACACAACAGCTTTGCATACAGCTGCAACACAAGGACATATTGAGATAGTGAAATTCCTATTGGAAGCAGGAAGTAGTTTGGCAACCATTGCTAAAAGTAACGGGAAAACGGCTCTTCATTCTGCTGCAAGAAATGGACATTTGGAGGTTGTGAAAGCACTTCTTGAGAAAGAGCCAGGAGTTGCAACAAGGACTGATAAAAAGGGCCAGACAGCACTTCACATGGCAGTAAAGGGACAAAATCTTGAGGTAGTGGAAGAGTTGATAAAAGCAGATCCTTCAACAATAAACATGGTTGATACTAAAGGAAACACAACATTGCATATAGCAACCAGGAAAGCCAGAACTCGGATTGTAAATATGCTTCTAGGACAAAAGGAAACAAATGTAAGTGCTGTTAACAGATCAGGTGAAACAGCAGTAGACACTGCTGAGAAAATAGGGAACCAGGATGTTAAAGCCCTTCTATTAGAGCATGGTGTTCAAAGTGCAAAGTCTATAAAACCACCACAAGGATCAACAAGCACAGCTCGCGAGTTAAAGCAAACTGTAAGCGACATAAAGCACGAGGTACATTACCAATTAGAACACACACGCCAGACACGAAAACGTGTTCAAGGAATCGCAAAACGTATCAACAAGATGAATACAGAAGGACTCAACAATGCAATAAACTCGACAACCGTTGTGGCAGTACTAATAGCAACGGTTGCATTTGCTGCGATTTTCACTGTCCCTGGTCAATTTGTTGATGATCCAAACAATATTCCAGAAGGGATGATACTTGGTGAAGCAAATATATCTCCAGAAGCACCATTTATAATTTTCTTTGTATTTGATTCTATTGCACTCTTCATTTCTCTGGCTGTTGTGGTTGTTCAAACTTCGGTTGTCGTTATAGAAAGCAAAGCAAAGAAACAAATGGCAGCTATAATTAATAAGCTAATGTGGTTGGCTTGTGTGCTTATTTCTGTGGCATTTTTAGCATTGTCATTTGTTGTTGTTGGAAAGGAAGAAAAATGGTTGGCAATTTTTGTTACCATTATAGGAGCAACTATAATGGCCACAACTTTGGGAACTATGTGTTATTGGGTCATTAAGCATCGAATTGAGGCATCAAACTTGAGGAACATTAGGAAATCTTCAATGGAAAGCAAATCAAAGTCTTTTTCAGTGACAGCTTTGTCAGATTCTGAGCTAATGAACAATGAATTTAAGAAAATGTATGCTATTTAG |
| Protein sequence |
>MsG0280010984.01.T01 MGESSMATPMVHTPRKKMTKQLTGKRDDTPLHSAARAGNLDVVRDILNDAQEDELLELLARQNQDGETALYVAAEYGYIDVVRGMIQYYDLAGAGIKARNGFDAFHIAAKQGDIDILKILMEVHPELSMTVDPSNTTALHTAATQGHIEIVKFLLEAGSSLATIAKSNGKTALHSAARNGHLEVVKALLEKEPGVATRTDKKGQTALHMAVKGQNLEVVEELIKADPSTINMVDTKGNTTLHIATRKARTRIVNMLLGQKETNVSAVNRSGETAVDTAEKIGNQDVKALLLEHGVQSAKSIKPPQGSTSTARELKQTVSDIKHEVHYQLEHTRQTRKRVQGIAKRINKMNTEGLNNAINSTTVVAVLIATVAFAAIFTVPGQFVDDPNNIPEGMILGEANISPEAPFIIFFVFDSIALFISLAVVVVQTSVVVIESKAKKQMAAIINKLMWLACVLISVAFLALSFVVVGKEEKWLAIFVTIIGATIMATTLGTMCYWVIKHRIEASNLRNIRKSSMESKSKSFSVTALSDSELMNNEFKKMYAI* |
