AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011026.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011026.01.T01 MTR_2g096900 81.379 145 5 1 1 123 1 145 1.55e-58 184
MsG0280011026.01.T01 MTR_2g096920 72.727 143 17 1 1 121 1 143 4.30e-54 173
MsG0280011026.01.T01 MTR_2g096920 69.000 100 9 1 1 78 1 100 9.92e-39 132
MsG0280011026.01.T01 MTR_1g093780 43.684 190 89 5 127 303 331 515 1.73e-32 125
MsG0280011026.01.T01 MTR_2g437210 43.810 105 50 3 202 303 123 221 7.46e-15 72.8
MsG0280011026.01.T01 MTR_7g083320 43.750 128 43 5 181 302 1 105 2.76e-14 68.2
MsG0280011026.01.T01 MTR_4g121900 29.221 154 60 3 18 122 24 177 4.16e-12 63.9
MsG0280011026.01.T01 MTR_2g074890 38.255 149 69 7 162 303 345 477 6.67e-12 66.2
MsG0280011026.01.T01 MTR_2g034550 27.097 155 63 2 18 122 26 180 3.75e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011026.01.T01 AT2G28900 53.846 143 44 2 1 121 1 143 4.67e-45 150

Find 99 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGATAATGAAGGAATTT+TGG 0.142163 2:-78570538 MsG0280011026.01.T01:CDS
ACATGACAGTCACAAATATT+TGG 0.169688 2:+78570289 None:intergenic
CCTTTGCCAATGTGAGTCTT+AGG 0.217782 2:+78570380 None:intergenic
ATGCCTCATAGCAGCATTTC+AGG 0.220503 2:-78572832 MsG0280011026.01.T01:CDS
CTTCTCAACCTCACTGTTGA+TGG 0.269257 2:-78572760 MsG0280011026.01.T01:CDS
CACAAAAGTGTGACCACTTA+TGG 0.284275 2:+78572972 None:intergenic
CATCCAAACTCGTTCCAAAA+AGG 0.284341 2:-78570330 MsG0280011026.01.T01:CDS
GCTGGCTGCTGAGTGGTTAC+TGG 0.311741 2:+78570571 None:intergenic
AGTCTGGGCAAAAGAGTTAT+AGG 0.327469 2:-78570611 MsG0280011026.01.T01:CDS
ATTGTAGTAGGTGCCATTAC+AGG 0.330295 2:-78571319 MsG0280011026.01.T01:CDS
ACTGCTGCAGCATTCATAAA+TGG 0.345573 2:-78571283 MsG0280011026.01.T01:CDS
GGAACAGCCAAAGACAAAAC+TGG 0.362261 2:+78570659 None:intergenic
CATGACAGTCACAAATATTT+GGG 0.363803 2:+78570290 None:intergenic
CAAATGTTGGACTCTGTCTC+GGG 0.375402 2:-78570725 MsG0280011026.01.T01:CDS
GTTGATGGTTTCTTGAAGAT+TGG 0.388016 2:-78572745 MsG0280011026.01.T01:CDS
CTTAGTCTTCCTACCAGGCT+TGG 0.394054 2:+78570415 None:intergenic
TGTGGATGAAGATTTGAGTC+TGG 0.401407 2:-78570627 MsG0280011026.01.T01:CDS
CTCAGACATTAACACTGATA+TGG 0.403641 2:-78570489 MsG0280011026.01.T01:CDS
AGATGCTATGTCAGTATGTT+TGG 0.419644 2:+78570442 None:intergenic
AGCTACCAGCTTGAATGTTA+AGG 0.419870 2:+78572900 None:intergenic
TGTTGGACTCTGTCTCGGGG+GGG 0.422135 2:-78570721 MsG0280011026.01.T01:CDS
GGATCCGTGGCACCAGAGAC+TGG 0.423846 2:-78571709 MsG0280011026.01.T01:CDS
ACATTTGGAGGTGCAGTGAC+AGG 0.426302 2:-78571382 MsG0280011026.01.T01:CDS
GAACACCGAACCACCAAACT+TGG 0.430785 2:+78570689 None:intergenic
ATGTTGGACTCTGTCTCGGG+GGG 0.433498 2:-78570722 MsG0280011026.01.T01:CDS
GTATATGTTGGAACAGAATA+CGG 0.434501 2:-78571740 MsG0280011026.01.T01:CDS
CTTTGCCAATGTGAGTCTTA+GGG 0.439223 2:+78570381 None:intergenic
CGCAATTGCAGCCCCTGTAA+TGG 0.440225 2:+78571306 None:intergenic
AGGTGCAGTGACAGGGGCTC+TGG 0.442203 2:-78571374 MsG0280011026.01.T01:CDS
TGCTTCTTAGTCTTCCTACC+AGG 0.442253 2:+78570410 None:intergenic
AGAGTTATAGGTGGAAAGTC+TGG 0.443810 2:-78570599 MsG0280011026.01.T01:CDS
CACCTATTCCATTGGAATCA+AGG 0.445195 2:-78570781 MsG0280011026.01.T01:CDS
GTGATCCAAGTTTGGTGGTT+CGG 0.457160 2:-78570694 MsG0280011026.01.T01:CDS
GCAAATGTTGGACTCTGTCT+CGG 0.463965 2:-78570726 MsG0280011026.01.T01:CDS
ATTTGCTTGTTAGAAACTAT+AGG 0.472776 2:+78570743 None:intergenic
GGCCTTGATTCCAATGGAAT+AGG 0.478081 2:+78570779 None:intergenic
ACCCTTTCGAGCAATATGAT+AGG 0.487140 2:+78572600 None:intergenic
TGAGAGAATTACCCAGTCTC+TGG 0.488901 2:+78571697 None:intergenic
GTCAATGACCACATCGACCT+TGG 0.493655 2:+78572795 None:intergenic
ACCTATCATATTGCTCGAAA+GGG 0.494769 2:-78572601 MsG0280011026.01.T01:intron
AAGGCAGACAAGATTGTAGT+AGG 0.498217 2:-78571331 MsG0280011026.01.T01:CDS
GGGGTGTGATCCAAGTTTGG+TGG 0.499618 2:-78570699 MsG0280011026.01.T01:CDS
GCTATAGCTGGTGTATATGT+TGG 0.502697 2:-78571752 MsG0280011026.01.T01:CDS
ATCTTCATCCACAGAAGTGA+CGG 0.511130 2:+78570637 None:intergenic
CTGCAGCATTCATAAATGGC+TGG 0.512276 2:-78571279 MsG0280011026.01.T01:CDS
TACCTATCATATTGCTCGAA+AGG 0.512817 2:-78572602 MsG0280011026.01.T01:CDS
ATCTGCAGCCAACAATGAAA+AGG 0.513448 2:-78571350 MsG0280011026.01.T01:CDS
TCAATGACCACATCGACCTT+GGG 0.517343 2:+78572796 None:intergenic
TTGTAGTAGGTGCCATTACA+GGG 0.523008 2:-78571318 MsG0280011026.01.T01:CDS
AGTTTCTAACAAGCAAATGT+TGG 0.526698 2:-78570738 MsG0280011026.01.T01:CDS
GCAAGATGCACCTATTCCAT+TGG 0.528869 2:-78570789 MsG0280011026.01.T01:CDS
AGGATCAGTGTCTTCTCCCA+AGG 0.530325 2:-78572812 MsG0280011026.01.T01:CDS
TATATGTTGGAACAGAATAC+GGG 0.530549 2:-78571739 MsG0280011026.01.T01:CDS
GGCTGTTCCCGTCACTTCTG+TGG 0.535219 2:-78570645 MsG0280011026.01.T01:CDS
GAACAGCCAAAGACAAAACT+GGG 0.536588 2:+78570660 None:intergenic
CACATTGGCAAAGGTTCTGA+TGG 0.538523 2:-78570371 MsG0280011026.01.T01:CDS
ACCTTCCTTAACATTCAAGC+TGG 0.539605 2:-78572905 MsG0280011026.01.T01:five_prime_UTR
GAGGATGCTTGCTTAGAGCC+AGG 0.540490 2:-78570818 MsG0280011026.01.T01:CDS
CATCTAACACCAAGCCTGGT+AGG 0.540647 2:-78570424 MsG0280011026.01.T01:CDS
CATTTGGAGGTGCAGTGACA+GGG 0.546047 2:-78571381 MsG0280011026.01.T01:CDS
TTGTTAGAAACTATAGGTAG+AGG 0.547056 2:+78570749 None:intergenic
TAAAAGTGTGCAGATAATGA+AGG 0.550725 2:-78570546 MsG0280011026.01.T01:CDS
ATAGCATCTAACACCAAGCC+TGG 0.555962 2:-78570428 MsG0280011026.01.T01:CDS
ACGCTTACAGGAGCTATAGC+TGG 0.557663 2:-78571764 MsG0280011026.01.T01:intron
GGTCGATGTGGTCATTGACA+TGG 0.564917 2:-78572791 MsG0280011026.01.T01:CDS
GATCCTGAAATGCTGCTATG+AGG 0.565334 2:+78572829 None:intergenic
ATCCTCTGCAAGTGCCCTTG+TGG 0.568133 2:+78572624 None:intergenic
GTGGATGAAGATTTGAGTCT+GGG 0.570778 2:-78570626 MsG0280011026.01.T01:CDS
CTTTCAATCACAGCCATAAG+TGG 0.570799 2:-78572985 None:intergenic
ACTATAGGTAGAGGCTGAGA+GGG 0.572049 2:+78570758 None:intergenic
AACTATAGGTAGAGGCTGAG+AGG 0.577361 2:+78570757 None:intergenic
TGTCACTGCACCTCCAAATG+TGG 0.580744 2:+78571384 None:intergenic
TGAGAGGGCCTTGATTCCAA+TGG 0.582673 2:+78570773 None:intergenic
AAATGTTGGACTCTGTCTCG+GGG 0.583414 2:-78570724 MsG0280011026.01.T01:CDS
TTACCCAGTCTCTGGTGCCA+CGG 0.593471 2:+78571705 None:intergenic
GATCCGTGGCACCAGAGACT+GGG 0.594640 2:-78571708 MsG0280011026.01.T01:intron
AGCTTGAATGTTAAGGAAGG+TGG 0.594953 2:+78572907 None:intergenic
AGCCACAAGGGCACTTGCAG+AGG 0.596020 2:-78572626 MsG0280011026.01.T01:CDS
CATATCAGTGTTAATGTCTG+AGG 0.597268 2:+78570490 None:intergenic
AATGTTGGACTCTGTCTCGG+GGG 0.601976 2:-78570723 MsG0280011026.01.T01:CDS
AAAAGCCCTAAGACTCACAT+TGG 0.608767 2:-78570386 MsG0280011026.01.T01:CDS
TCTTCATCCACAGAAGTGAC+GGG 0.611855 2:+78570638 None:intergenic
TACGGGGTGGAGAGGATCCG+TGG 0.613293 2:-78571722 MsG0280011026.01.T01:CDS
ATATACTACTATATAGCAAG+AGG 0.614367 2:+78572943 None:intergenic
CCTAAGACTCACATTGGCAA+AGG 0.618293 2:-78570380 MsG0280011026.01.T01:CDS
GTCTTCTCCCAAGGTCGATG+TGG 0.618468 2:-78572803 MsG0280011026.01.T01:CDS
GAACAGAATACGGGGTGGAG+AGG 0.619958 2:-78571730 MsG0280011026.01.T01:CDS
GTCGATGTGGTCATTGACAT+GGG 0.620754 2:-78572790 MsG0280011026.01.T01:CDS
GGTGCATCTTGCTCTAGACC+TGG 0.627077 2:+78570800 None:intergenic
ACCAGCTTGAATGTTAAGGA+AGG 0.629164 2:+78572904 None:intergenic
TCAACAGTGAGGTTGAGAAG+TGG 0.636785 2:+78572763 None:intergenic
ATTTGGAGGTGCAGTGACAG+GGG 0.640699 2:-78571380 MsG0280011026.01.T01:CDS
CTGGGCAAAAGAGTTATAGG+TGG 0.668810 2:-78570608 MsG0280011026.01.T01:CDS
GGTAGCTAAACAAAGCAAAG+AGG 0.681272 2:-78572884 MsG0280011026.01.T01:five_prime_UTR
TGTAGTAGGTGCCATTACAG+GGG 0.704939 2:-78571317 MsG0280011026.01.T01:CDS
TCAAGAAACCATCAACAGTG+AGG 0.715759 2:+78572752 None:intergenic
TGTTGGAACAGAATACGGGG+TGG 0.732754 2:-78571735 MsG0280011026.01.T01:CDS
AACAGCCAAAGACAAAACTG+GGG 0.763097 2:+78570661 None:intergenic
ATATGTTGGAACAGAATACG+GGG 0.764667 2:-78571738 MsG0280011026.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTAAAAATTATATTTT+AGG + Chr2:78570967-78570986 None:intergenic 0.0%
!!! AAATTATATTTTAGGAGTAA+AGG + Chr2:78570959-78570978 None:intergenic 15.0%
!! AAAAGATGAAAACTTTGATT+CGG - Chr2:78570741-78570760 MsG0280011026.01.T01:CDS 20.0%
!! AACTTAAATTGAACAATAGA+AGG + Chr2:78571810-78571829 None:intergenic 20.0%
!! ATATATATCATGATTTCAAC+AGG + Chr2:78572343-78572362 None:intergenic 20.0%
!! CATCAAAATTATTACATAAC+CGG + Chr2:78572107-78572126 None:intergenic 20.0%
!! TATATATCATGATTTCAACA+GGG + Chr2:78572342-78572361 None:intergenic 20.0%
!! TATTATTCCATGTTTGAAAA+GGG - Chr2:78572050-78572069 MsG0280011026.01.T01:intron 20.0%
!! TATTTATCAAAGTTAAAGCA+TGG + Chr2:78571195-78571214 None:intergenic 20.0%
!!! AAATGTGTTAACTTTGTTTT+AGG - Chr2:78571772-78571791 MsG0280011026.01.T01:intron 20.0%
!!! ATGCTTTAACTTTGATAAAT+AGG - Chr2:78571194-78571213 MsG0280011026.01.T01:intron 20.0%
!!! CGTTTTCATAAATATTATTG+CGG - Chr2:78572269-78572288 MsG0280011026.01.T01:intron 20.0%
!!! TTATCATGATTGATTCATTT+GGG - Chr2:78570929-78570948 MsG0280011026.01.T01:intron 20.0%
!!! TTCAGTATTTTTTAAAGCAA+CGG + Chr2:78572381-78572400 None:intergenic 20.0%
!!! TTTTGGAGATTTTAACAAAA+GGG + Chr2:78570725-78570744 None:intergenic 20.0%
! AAAGATGAAAACTTTGATTC+GGG - Chr2:78570742-78570761 MsG0280011026.01.T01:CDS 25.0%
! AGACAAAATGTCTATTGAAT+GGG + Chr2:78571331-78571350 None:intergenic 25.0%
! AGAGATTAGATGTCATTATA+TGG + Chr2:78571689-78571708 None:intergenic 25.0%
! ATATACTACTATATAGCAAG+AGG + Chr2:78570334-78570353 None:intergenic 25.0%
! ATTATTCCATGTTTGAAAAG+GGG - Chr2:78572051-78572070 MsG0280011026.01.T01:intron 25.0%
! CAATAATGTGATTTGAACTT+GGG - Chr2:78571048-78571067 MsG0280011026.01.T01:intron 25.0%
! CTATTATTCCATGTTTGAAA+AGG - Chr2:78572049-78572068 MsG0280011026.01.T01:intron 25.0%
! GAAAACACTTAAAATAGACT+TGG - Chr2:78571611-78571630 MsG0280011026.01.T01:intron 25.0%
! TAGACAAAATGTCTATTGAA+TGG + Chr2:78571332-78571351 None:intergenic 25.0%
!! ACTTTTAGTATAAAGAAACC+TGG - Chr2:78572193-78572212 MsG0280011026.01.T01:intron 25.0%
!! ATTTGCTTGTTAGAAACTAT+AGG + Chr2:78572534-78572553 None:intergenic 25.0%
!! CGGTTATGTAATAATTTTGA+TGG - Chr2:78572105-78572124 MsG0280011026.01.T01:intron 25.0%
!! CTTATCATGATTGATTCATT+TGG - Chr2:78570928-78570947 MsG0280011026.01.T01:intron 25.0%
!! CTTTTAGTATAAAGAAACCT+GGG - Chr2:78572194-78572213 MsG0280011026.01.T01:intron 25.0%
!! GAATCAAAGTTTTCATCTTT+TGG + Chr2:78570742-78570761 None:intergenic 25.0%
!! TATCATGATTGATTCATTTG+GGG - Chr2:78570930-78570949 MsG0280011026.01.T01:intron 25.0%
!!! CTTTTGGAGATTTTAACAAA+AGG + Chr2:78570726-78570745 None:intergenic 25.0%
AAATGAGAGAATCCGAATTT+AGG + Chr2:78571111-78571130 None:intergenic 30.0%
AAATTGAACAATAGAAGGCA+TGG + Chr2:78571805-78571824 None:intergenic 30.0%
AAATTTAAACATTAGCAGCC+AGG - Chr2:78572232-78572251 MsG0280011026.01.T01:intron 30.0%
AACGTTAAACTTTATTCACC+TGG + Chr2:78572253-78572272 None:intergenic 30.0%
AATGAGAGAATCCGAATTTA+GGG + Chr2:78571110-78571129 None:intergenic 30.0%
AGTTTCTAACAAGCAAATGT+TGG - Chr2:78572536-78572555 MsG0280011026.01.T01:intron 30.0%
CTGAAGAAGACATTTAAAGA+AGG - Chr2:78571396-78571415 MsG0280011026.01.T01:intron 30.0%
GAGAATCTAAATCCTGAAAA+GGG - Chr2:78571298-78571317 MsG0280011026.01.T01:CDS 30.0%
GCAATAATGTGATTTGAACT+TGG - Chr2:78571047-78571066 MsG0280011026.01.T01:intron 30.0%
GTATATGTTGGAACAGAATA+CGG - Chr2:78571534-78571553 MsG0280011026.01.T01:intron 30.0%
TATATGTTGGAACAGAATAC+GGG - Chr2:78571535-78571554 MsG0280011026.01.T01:intron 30.0%
TCCTAGAAAAGAATAATGAC+AGG - Chr2:78571079-78571098 MsG0280011026.01.T01:intron 30.0%
TGAGAATCTAAATCCTGAAA+AGG - Chr2:78571297-78571316 MsG0280011026.01.T01:CDS 30.0%
TTGAGCTGTTTGATCAAAAT+CGG - Chr2:78571164-78571183 MsG0280011026.01.T01:intron 30.0%
TTGTTAGAAACTATAGGTAG+AGG + Chr2:78572528-78572547 None:intergenic 30.0%
TTTCTGTGTATATCCTAAAG+AGG - Chr2:78572437-78572456 MsG0280011026.01.T01:intron 30.0%
! ATTTAAAGAAGGTGCATACT+GGG - Chr2:78571407-78571426 MsG0280011026.01.T01:intron 30.0%
! CAGATAATGAAGGAATTTTG+GGG - Chr2:78572738-78572757 MsG0280011026.01.T01:intron 30.0%
! GCAGATAATGAAGGAATTTT+GGG - Chr2:78572737-78572756 MsG0280011026.01.T01:intron 30.0%
! TAAAAGTGTGCAGATAATGA+AGG - Chr2:78572728-78572747 MsG0280011026.01.T01:intron 30.0%
! TCATTATCTGCACACTTTTA+AGG + Chr2:78572728-78572747 None:intergenic 30.0%
! TCCTGTCATTATTCTTTTCT+AGG + Chr2:78571083-78571102 None:intergenic 30.0%
! TGCAGATAATGAAGGAATTT+TGG - Chr2:78572736-78572755 MsG0280011026.01.T01:intron 30.0%
AACAAGACCTCTCATTAACA+AGG + Chr2:78571372-78571391 None:intergenic 35.0%
ACAAGGAACAAAGCATATCA+TGG + Chr2:78571355-78571374 None:intergenic 35.0%
ACCTATCATATTGCTCGAAA+GGG - Chr2:78570673-78570692 MsG0280011026.01.T01:CDS 35.0%
ACTGACATATCAAGATCAGA+TGG + Chr2:78571245-78571264 None:intergenic 35.0%
ACTGGGTAATTCTCTCAATA+GGG - Chr2:78571583-78571602 MsG0280011026.01.T01:intron 35.0%
AGATGCTATGTCAGTATGTT+TGG + Chr2:78572835-78572854 None:intergenic 35.0%
ATATGTTGGAACAGAATACG+GGG - Chr2:78571536-78571555 MsG0280011026.01.T01:intron 35.0%
ATCCAAACTCGTTCCAAAAA+GGG - Chr2:78572945-78572964 MsG0280011026.01.T01:five_prime_UTR 35.0%
ATGAGAGAATCCGAATTTAG+GGG + Chr2:78571109-78571128 None:intergenic 35.0%
CAAAAGGGTTATAGCAAAAG+TGG + Chr2:78570710-78570729 None:intergenic 35.0%
CATATCAGTGTTAATGTCTG+AGG + Chr2:78572787-78572806 None:intergenic 35.0%
CTCAGACATTAACACTGATA+TGG - Chr2:78572785-78572804 MsG0280011026.01.T01:CDS 35.0%
CTTTGTTCCTTGTTAATGAG+AGG - Chr2:78571362-78571381 MsG0280011026.01.T01:CDS 35.0%
GTACAGAAAAATGCCACATT+TGG - Chr2:78571877-78571896 MsG0280011026.01.T01:intron 35.0%
TAAATGGCTGGAGTTTCTAA+TGG - Chr2:78572007-78572026 MsG0280011026.01.T01:intron 35.0%
TAATGTGATTTGAACTTGGG+AGG - Chr2:78571051-78571070 MsG0280011026.01.T01:intron 35.0%
TACCTATCATATTGCTCGAA+AGG - Chr2:78570672-78570691 MsG0280011026.01.T01:CDS 35.0%
TCTGTACATCAAAAGACCAA+GGG + Chr2:78571864-78571883 None:intergenic 35.0%
TTCAGCTTTCTGTCAATTCT+TGG - Chr2:78572150-78572169 MsG0280011026.01.T01:intron 35.0%
TTCTAGATCATGAGATGAAG+GGG - Chr2:78572292-78572311 MsG0280011026.01.T01:intron 35.0%
TTCTGTACATCAAAAGACCA+AGG + Chr2:78571865-78571884 None:intergenic 35.0%
TTGGTATGTATGCATCTTTG+AGG - Chr2:78571025-78571044 MsG0280011026.01.T01:intron 35.0%
! ATTTTCCTTTCTACGCTTAC+AGG - Chr2:78571498-78571517 MsG0280011026.01.T01:intron 35.0%
! CATTTAAAGAAGGTGCATAC+TGG - Chr2:78571406-78571425 MsG0280011026.01.T01:intron 35.0%
! GTTCTAGATCATGAGATGAA+GGG - Chr2:78572291-78572310 MsG0280011026.01.T01:intron 35.0%
! TTCTGTCAATTCTTGGTCTT+TGG - Chr2:78572157-78572176 MsG0280011026.01.T01:intron 35.0%
! TTTAAAGAAGGTGCATACTG+GGG - Chr2:78571408-78571427 MsG0280011026.01.T01:intron 35.0%
!! GCTTTTGATTACACCCTTTT+TGG + Chr2:78572961-78572980 None:intergenic 35.0%
!! GTTGATGGTTTCTTGAAGAT+TGG - Chr2:78570529-78570548 MsG0280011026.01.T01:CDS 35.0%
!! TAGAGAGAGCACTTTATTTC+TGG - Chr2:78571654-78571673 MsG0280011026.01.T01:intron 35.0%
AAAAGCCCTAAGACTCACAT+TGG - Chr2:78572888-78572907 MsG0280011026.01.T01:five_prime_UTR 40.0%
AACAGCCAAAGACAAAACTG+GGG + Chr2:78572616-78572635 None:intergenic 40.0%
AAGGCAGACAAGATTGTAGT+AGG - Chr2:78571943-78571962 MsG0280011026.01.T01:intron 40.0%
ACCAGCTTGAATGTTAAGGA+AGG + Chr2:78570373-78570392 None:intergenic 40.0%
ACCCTTTCGAGCAATATGAT+AGG + Chr2:78570677-78570696 None:intergenic 40.0%
ACCTTCCTTAACATTCAAGC+TGG - Chr2:78570369-78570388 MsG0280011026.01.T01:CDS 40.0%
ACTGCTGCAGCATTCATAAA+TGG - Chr2:78571991-78572010 MsG0280011026.01.T01:intron 40.0%
AGAGTTATAGGTGGAAAGTC+TGG - Chr2:78572675-78572694 MsG0280011026.01.T01:intron 40.0%
AGCTACCAGCTTGAATGTTA+AGG + Chr2:78570377-78570396 None:intergenic 40.0%
AGCTTGAATGTTAAGGAAGG+TGG + Chr2:78570370-78570389 None:intergenic 40.0%
AGTCTGGGCAAAAGAGTTAT+AGG - Chr2:78572663-78572682 MsG0280011026.01.T01:intron 40.0%
ATCTAAATCCTGAAAAGGGC+AGG - Chr2:78571302-78571321 MsG0280011026.01.T01:CDS 40.0%
ATCTGCAGCCAACAATGAAA+AGG - Chr2:78571924-78571943 MsG0280011026.01.T01:intron 40.0%
ATCTTCATCCACAGAAGTGA+CGG + Chr2:78572640-78572659 None:intergenic 40.0%
CACCTATTCCATTGGAATCA+AGG - Chr2:78572493-78572512 MsG0280011026.01.T01:intron 40.0%
CAGAAAAATGCCACATTTGG+AGG - Chr2:78571880-78571899 MsG0280011026.01.T01:intron 40.0%
CATCCAAACTCGTTCCAAAA+AGG - Chr2:78572944-78572963 MsG0280011026.01.T01:five_prime_UTR 40.0%
CTTCTAAACATGTCTGTTCC+AGG - Chr2:78570845-78570864 MsG0280011026.01.T01:intron 40.0%
CTTTGCCAATGTGAGTCTTA+GGG + Chr2:78572896-78572915 None:intergenic 40.0%
GAACAGCCAAAGACAAAACT+GGG + Chr2:78572617-78572636 None:intergenic 40.0%
GACTGGGTAATTCTCTCAAT+AGG - Chr2:78571582-78571601 MsG0280011026.01.T01:intron 40.0%
GCTATAGCTGGTGTATATGT+TGG - Chr2:78571522-78571541 MsG0280011026.01.T01:intron 40.0%
GGTAGCTAAACAAAGCAAAG+AGG - Chr2:78570390-78570409 MsG0280011026.01.T01:CDS 40.0%
GTGGATGAAGATTTGAGTCT+GGG - Chr2:78572648-78572667 MsG0280011026.01.T01:intron 40.0%
TCAAAAGACCAAGGGAACAA+CGG + Chr2:78571856-78571875 None:intergenic 40.0%
TCAAGAAACCATCAACAGTG+AGG + Chr2:78570525-78570544 None:intergenic 40.0%
TCTAAGCAAGCATCCTCTTT+AGG + Chr2:78572453-78572472 None:intergenic 40.0%
TGTGGATGAAGATTTGAGTC+TGG - Chr2:78572647-78572666 MsG0280011026.01.T01:intron 40.0%
TTCACGTAATCACACACTTG+CGG + Chr2:78571274-78571293 None:intergenic 40.0%
TTGAAAAGGGGTTGCACATT+TGG - Chr2:78572063-78572082 MsG0280011026.01.T01:intron 40.0%
! CACAAAAGTGTGACCACTTA+TGG + Chr2:78570305-78570324 None:intergenic 40.0%
! CATGATTGATTCATTTGGGG+TGG - Chr2:78570933-78570952 MsG0280011026.01.T01:intron 40.0%
! CTTGTCTGCCTTTTCATTGT+TGG + Chr2:78571935-78571954 None:intergenic 40.0%
! GTCTCTTTTGTCTGTCTTAC+CGG - Chr2:78572085-78572104 MsG0280011026.01.T01:intron 40.0%
!! ATTGTAGTAGGTGCCATTAC+AGG - Chr2:78571955-78571974 MsG0280011026.01.T01:intron 40.0%
!! GGTTCTAGATCATGAGATGA+AGG - Chr2:78572290-78572309 MsG0280011026.01.T01:intron 40.0%
!! TTGTAGTAGGTGCCATTACA+GGG - Chr2:78571956-78571975 MsG0280011026.01.T01:intron 40.0%
!!! TATCTGCACACTTTTAAGGC+TGG + Chr2:78572724-78572743 None:intergenic 40.0%
AAAGAAACCTGGGCATGAAC+AGG - Chr2:78572204-78572223 MsG0280011026.01.T01:intron 45.0%
AAATGTTGGACTCTGTCTCG+GGG - Chr2:78572550-78572569 MsG0280011026.01.T01:intron 45.0%
AACTATAGGTAGAGGCTGAG+AGG + Chr2:78572520-78572539 None:intergenic 45.0%
ACTATAGGTAGAGGCTGAGA+GGG + Chr2:78572519-78572538 None:intergenic 45.0%
ATAGCATCTAACACCAAGCC+TGG - Chr2:78572846-78572865 MsG0280011026.01.T01:exon 45.0%
ATGCCTCATAGCAGCATTTC+AGG - Chr2:78570442-78570461 MsG0280011026.01.T01:CDS 45.0%
CAAATGTTGGACTCTGTCTC+GGG - Chr2:78572549-78572568 MsG0280011026.01.T01:intron 45.0%
CCTAAGACTCACATTGGCAA+AGG - Chr2:78572894-78572913 MsG0280011026.01.T01:five_prime_UTR 45.0%
CCTTTGCCAATGTGAGTCTT+AGG + Chr2:78572897-78572916 None:intergenic 45.0%
CTGCAGCATTCATAAATGGC+TGG - Chr2:78571995-78572014 MsG0280011026.01.T01:intron 45.0%
CTGGGCAAAAGAGTTATAGG+TGG - Chr2:78572666-78572685 MsG0280011026.01.T01:intron 45.0%
CTTCTCAACCTCACTGTTGA+TGG - Chr2:78570514-78570533 MsG0280011026.01.T01:CDS 45.0%
GACAGGAAAGCCCCTAAATT+CGG - Chr2:78571096-78571115 MsG0280011026.01.T01:intron 45.0%
GATCCTGAAATGCTGCTATG+AGG + Chr2:78570448-78570467 None:intergenic 45.0%
GATTCTTACCGTTGTTCCCT+TGG - Chr2:78571845-78571864 MsG0280011026.01.T01:intron 45.0%
GCAAATGTTGGACTCTGTCT+CGG - Chr2:78572548-78572567 MsG0280011026.01.T01:intron 45.0%
GCAAGATGCACCTATTCCAT+TGG - Chr2:78572485-78572504 MsG0280011026.01.T01:intron 45.0%
GGAACAGCCAAAGACAAAAC+TGG + Chr2:78572618-78572637 None:intergenic 45.0%
GGCCTTGATTCCAATGGAAT+AGG + Chr2:78572498-78572517 None:intergenic 45.0%
GTCGATGTGGTCATTGACAT+GGG - Chr2:78570484-78570503 MsG0280011026.01.T01:CDS 45.0%
TAGCTCCTGTAAGCGTAGAA+AGG + Chr2:78571506-78571525 None:intergenic 45.0%
TCAACAGTGAGGTTGAGAAG+TGG + Chr2:78570514-78570533 None:intergenic 45.0%
TCAATGACCACATCGACCTT+GGG + Chr2:78570481-78570500 None:intergenic 45.0%
TCACTACTTGAGACACTTCC+TGG + Chr2:78570866-78570885 None:intergenic 45.0%
TCTTCATCCACAGAAGTGAC+GGG + Chr2:78572639-78572658 None:intergenic 45.0%
TGAGAGAATTACCCAGTCTC+TGG + Chr2:78571580-78571599 None:intergenic 45.0%
TTGAAAACCTGTTCATGCCC+AGG + Chr2:78572214-78572233 None:intergenic 45.0%
! CACATTGGCAAAGGTTCTGA+TGG - Chr2:78572903-78572922 MsG0280011026.01.T01:five_prime_UTR 45.0%
! GTGATCCAAGTTTGGTGGTT+CGG - Chr2:78572580-78572599 MsG0280011026.01.T01:intron 45.0%
! GTGCAACCCCTTTTCAAACA+TGG + Chr2:78572060-78572079 None:intergenic 45.0%
! TGCTTCTTAGTCTTCCTACC+AGG + Chr2:78572867-78572886 None:intergenic 45.0%
!! TAGCACCCCAGTTTTGTCTT+TGG - Chr2:78572608-78572627 MsG0280011026.01.T01:CDS 45.0%
!! TGTAGTAGGTGCCATTACAG+GGG - Chr2:78571957-78571976 MsG0280011026.01.T01:intron 45.0%
!!! CACCCTTTTTGGAACGAGTT+TGG + Chr2:78572950-78572969 None:intergenic 45.0%
!!! TTTTCAGGTTGCAGCCACAA+GGG - Chr2:78570636-78570655 MsG0280011026.01.T01:CDS 45.0%
!!! TTTTTCAGGTTGCAGCCACA+AGG - Chr2:78570635-78570654 MsG0280011026.01.T01:CDS 45.0%
!! AAAAATAAAAAATAAATAGT+TGG + Chr2:78570616-78570635 None:intergenic 5.0%
!! ATATATAAATTACTTATAAT+TGG + Chr2:78571006-78571025 None:intergenic 5.0%
!! ATTATAAGTAATTTATATAT+TGG - Chr2:78571006-78571025 MsG0280011026.01.T01:intron 5.0%
!!! AAATAATAATATATTGATTT+TGG + Chr2:78570778-78570797 None:intergenic 5.0%
!!! TTATTTTTTATTTTTTTTTC+AGG - Chr2:78570621-78570640 MsG0280011026.01.T01:CDS 5.0%
AAGGGGACGTGTTGTAAGGT+AGG - Chr2:78572309-78572328 MsG0280011026.01.T01:intron 50.0%
AATGTTGGACTCTGTCTCGG+GGG - Chr2:78572551-78572570 MsG0280011026.01.T01:intron 50.0%
ACGCTTACAGGAGCTATAGC+TGG - Chr2:78571510-78571529 MsG0280011026.01.T01:intron 50.0%
AGGATCAGTGTCTTCTCCCA+AGG - Chr2:78570462-78570481 MsG0280011026.01.T01:CDS 50.0%
CATCTAACACCAAGCCTGGT+AGG - Chr2:78572850-78572869 MsG0280011026.01.T01:exon 50.0%
GAACACCGAACCACCAAACT+TGG + Chr2:78572588-78572607 None:intergenic 50.0%
GATGAAGGGGACGTGTTGTA+AGG - Chr2:78572305-78572324 MsG0280011026.01.T01:intron 50.0%
GGTCGATGTGGTCATTGACA+TGG - Chr2:78570483-78570502 MsG0280011026.01.T01:CDS 50.0%
GTCAATGACCACATCGACCT+TGG + Chr2:78570482-78570501 None:intergenic 50.0%
TGAGAGGGCCTTGATTCCAA+TGG + Chr2:78572504-78572523 None:intergenic 50.0%
TGTCACTGCACCTCCAAATG+TGG + Chr2:78571893-78571912 None:intergenic 50.0%
TGTTGGAACAGAATACGGGG+TGG - Chr2:78571539-78571558 MsG0280011026.01.T01:intron 50.0%
! ACATTTGGAGGTGCAGTGAC+AGG - Chr2:78571892-78571911 MsG0280011026.01.T01:intron 50.0%
! ATGGGATTCCTGCCCTTTTC+AGG + Chr2:78571313-78571332 None:intergenic 50.0%
! ATTTGGAGGTGCAGTGACAG+GGG - Chr2:78571894-78571913 MsG0280011026.01.T01:intron 50.0%
! CATTTGGAGGTGCAGTGACA+GGG - Chr2:78571893-78571912 MsG0280011026.01.T01:intron 50.0%
! CTTAGTCTTCCTACCAGGCT+TGG + Chr2:78572862-78572881 None:intergenic 50.0%
!!! TTTTAAGGCTGGCTGCTGAG+TGG + Chr2:78572713-78572732 None:intergenic 50.0%
ATCCTCTGCAAGTGCCCTTG+TGG + Chr2:78570653-78570672 None:intergenic 55.0%
ATGTTGGACTCTGTCTCGGG+GGG - Chr2:78572552-78572571 MsG0280011026.01.T01:intron 55.0%
CGCAATTGCAGCCCCTGTAA+TGG + Chr2:78571971-78571990 None:intergenic 55.0%
GAACAGAATACGGGGTGGAG+AGG - Chr2:78571544-78571563 MsG0280011026.01.T01:intron 55.0%
GAGGATGCTTGCTTAGAGCC+AGG - Chr2:78572456-78572475 MsG0280011026.01.T01:intron 55.0%
GGGGTGTGATCCAAGTTTGG+TGG - Chr2:78572575-78572594 MsG0280011026.01.T01:intron 55.0%
GTCTTCTCCCAAGGTCGATG+TGG - Chr2:78570471-78570490 MsG0280011026.01.T01:CDS 55.0%
! GGTGCATCTTGCTCTAGACC+TGG + Chr2:78572477-78572496 None:intergenic 55.0%
! TTACCCAGTCTCTGGTGCCA+CGG + Chr2:78571572-78571591 None:intergenic 55.0%
GATCCGTGGCACCAGAGACT+GGG - Chr2:78571566-78571585 MsG0280011026.01.T01:intron 60.0%
GCTGGCTGCTGAGTGGTTAC+TGG + Chr2:78572706-78572725 None:intergenic 60.0%
GGCTGTTCCCGTCACTTCTG+TGG - Chr2:78572629-78572648 MsG0280011026.01.T01:CDS 60.0%
TGTTGGACTCTGTCTCGGGG+GGG - Chr2:78572553-78572572 MsG0280011026.01.T01:intron 60.0%
! GGGGGGGTGTGATCCAAGTT+TGG - Chr2:78572572-78572591 MsG0280011026.01.T01:intron 60.0%
!! AGCCACAAGGGCACTTGCAG+AGG - Chr2:78570648-78570667 MsG0280011026.01.T01:CDS 60.0%
AGGTGCAGTGACAGGGGCTC+TGG - Chr2:78571900-78571919 MsG0280011026.01.T01:intron 65.0%
GGATCCGTGGCACCAGAGAC+TGG - Chr2:78571565-78571584 MsG0280011026.01.T01:intron 65.0%
GTTGGACTCTGTCTCGGGGG+GGG - Chr2:78572554-78572573 MsG0280011026.01.T01:intron 65.0%
TACGGGGTGGAGAGGATCCG+TGG - Chr2:78571552-78571571 MsG0280011026.01.T01:intron 65.0%
TTGGACTCTGTCTCGGGGGG+GGG - Chr2:78572555-78572574 MsG0280011026.01.T01:intron 65.0%
TGGACTCTGTCTCGGGGGGG+GGG - Chr2:78572556-78572575 MsG0280011026.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 78570298 78572998 78570298 ID=MsG0280011026.01;Name=MsG0280011026.01
Chr2 mRNA 78570298 78572998 78570298 ID=MsG0280011026.01.T01;Parent=MsG0280011026.01;Name=MsG0280011026.01.T01;_AED=0.50;_eAED=0.50;_QI=144|0.5|0.6|1|1|1|5|0|303
Chr2 exon 78570298 78570839 78570298 ID=MsG0280011026.01.T01:exon:17279;Parent=MsG0280011026.01.T01
Chr2 exon 78571278 78571413 78571278 ID=MsG0280011026.01.T01:exon:17278;Parent=MsG0280011026.01.T01
Chr2 exon 78571709 78571776 78571709 ID=MsG0280011026.01.T01:exon:17277;Parent=MsG0280011026.01.T01
Chr2 exon 78572602 78572653 78572602 ID=MsG0280011026.01.T01:exon:17276;Parent=MsG0280011026.01.T01
Chr2 exon 78572741 78572998 78572741 ID=MsG0280011026.01.T01:exon:17275;Parent=MsG0280011026.01.T01
Chr2 five_prime_UTR 78572855 78572998 78572855 ID=MsG0280011026.01.T01:five_prime_utr;Parent=MsG0280011026.01.T01
Chr2 CDS 78572741 78572854 78572741 ID=MsG0280011026.01.T01:cds;Parent=MsG0280011026.01.T01
Chr2 CDS 78572602 78572653 78572602 ID=MsG0280011026.01.T01:cds;Parent=MsG0280011026.01.T01
Chr2 CDS 78571709 78571776 78571709 ID=MsG0280011026.01.T01:cds;Parent=MsG0280011026.01.T01
Chr2 CDS 78571278 78571413 78571278 ID=MsG0280011026.01.T01:cds;Parent=MsG0280011026.01.T01
Chr2 CDS 78570298 78570839 78570298 ID=MsG0280011026.01.T01:cds;Parent=MsG0280011026.01.T01
Gene Sequence

>MsG0280011026.01.T01

ATGCCTCATAGCAGCATTTCAGGATCAGTGTCTTCTCCCAAGGTCGATGTGGTCATTGACATGGGCAATCCACTTCTCAACCTCACTGTTGATGGTTTCTTGAAGATTGGAGCTGTTGCAGCCACAAGGGCACTTGCAGAGGATACCTATCATATTGCTCGAAAGGGAGCTATAGCTGGTGTATATGTTGGAACAGAATACGGGGTGGAGAGGATCCGTGGCACCAGAGACTGGAAAAATGCCACATTTGGAGGTGCAGTGACAGGGGCTCTGGTATCTGCAGCCAACAATGAAAAGGCAGACAAGATTGTAGTAGGTGCCATTACAGGGGCTGCAATTGCGACTGCTGCAGCATTCATAAATGGCTGGAAGGATGCTTGCTTAGAGCCAGGTCTAGAGCAAGATGCACCTATTCCATTGGAATCAAGGCCCTCTCAGCCTCTACCTATAGTTTCTAACAAGCAAATGTTGGACTCTGTCTCGGGGGGGGGGTGTGATCCAAGTTTGGTGGTTCGGTGTTCTAGCACCCCAGTTTTGTCTTTGGCTGTTCCCGTCACTTCTGTGGATGAAGATTTGAGTCTGGGCAAAAGAGTTATAGGTGGAAAGTCTGGTGACACCAGTAACCACTCAGCAGCCAGCCTTAAAAGTGTGCAGATAATGAAGGAATTTTGGGGTGATGTTGATGACGACGACTCTGCCTCAGACATTAACACTGATATGGAGCATGCAGCTGACAGATACTTAGCCAAACATACTGACATAGCATCTAACACCAAGCCTGGTAGGAAGACTAAGAAGCAGAAAAGCCCTAAGACTCACATTGGCAAAGGTTCTGATGGCATCACAAGTAGTGAGCACATCCAAACTCGTTCCAAAAAGGGTGTAATCAAAAGCAATCCCAAATATTTGTGA
Protein sequence

>MsG0280011026.01.T01

MPHSSISGSVSSPKVDVVIDMGNPLLNLTVDGFLKIGAVAATRALAEDTYHIARKGAIAGVYVGTEYGVERIRGTRDWKNATFGGAVTGALVSAANNEKADKIVVGAITGAAIATAAAFINGWKDACLEPGLEQDAPIPLESRPSQPLPIVSNKQMLDSVSGGGCDPSLVVRCSSTPVLSLAVPVTSVDEDLSLGKRVIGGKSGDTSNHSAASLKSVQIMKEFWGDVDDDDSASDINTDMEHAADRYLAKHTDIASNTKPGRKTKKQKSPKTHIGKGSDGITSSEHIQTRSKKGVIKSNPKYL*