Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011069.01.T01 | XP_003597410.1 | 70.558 | 197 | 19 | 7 | 1 | 158 | 7 | 203 | 2.67E-75 | 238 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011069.01.T01 | Q2HS22 | 70.558 | 197 | 19 | 7 | 1 | 158 | 7 | 203 | 1.27e-75 | 238 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004704.01 | MsG0280011069.01 | 0.827350 | 1.642631e-54 | 1.581940e-51 |
| MsG0280011069.01 | MsG0380013100.01 | 0.825211 | 5.303653e-54 | 4.803327e-51 |
| MsG0280011069.01 | MsG0480019107.01 | 0.810813 | 9.563959e-51 | 5.822998e-48 |
| MsG0280011069.01 | MsG0480023291.01 | 0.817157 | 3.813970e-52 | 2.756325e-49 |
| MsG0280011069.01 | MsG0580025145.01 | 0.821802 | 3.326435e-53 | 2.734233e-50 |
| MsG0280011069.01 | MsG0880043185.01 | 0.801115 | 1.048394e-48 | 4.966277e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011069.01.T01 | MTR_2g097800 | 70.558 | 197 | 19 | 7 | 1 | 158 | 7 | 203 | 3.23e-79 | 238 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTACGCTAACGTGGTTAA+AGG | 0.245389 | 2:-79090700 | MsG0280011069.01.T01:CDS |
| AAATTAAAGCCATTGAATTA+AGG | 0.289984 | 2:-79090549 | MsG0280011069.01.T01:CDS |
| ATCTCTTCTTTAGCCTCATT+TGG | 0.290701 | 2:+79090505 | None:intergenic |
| CCTACGCTAACGTGGTTAAA+GGG | 0.390281 | 2:-79090699 | MsG0280011069.01.T01:CDS |
| TGATGAAAATCTATCAATAT+TGG | 0.390503 | 2:+79090594 | None:intergenic |
| CTCCGCCAGCTACACCTTGT+TGG | 0.404982 | 2:-79090732 | MsG0280011069.01.T01:CDS |
| GTCTTCCAACAAGGTGTAGC+TGG | 0.435910 | 2:+79090727 | None:intergenic |
| GAGATTCAGAAGCAAGGTAA+TGG | 0.461458 | 2:-79090487 | MsG0280011069.01.T01:CDS |
| GCTAACGTGGTTAAAGGGGT+TGG | 0.488779 | 2:-79090694 | MsG0280011069.01.T01:CDS |
| GCTCCATTGCAGACCAGTGG+TGG | 0.500459 | 2:-79090425 | MsG0280011069.01.T01:CDS |
| CTGCATCATTCCTCCACCAC+TGG | 0.505752 | 2:+79090412 | None:intergenic |
| TTGTAACCAGGGAGCTCAGA+AGG | 0.509786 | 2:-79090772 | MsG0280011069.01.T01:intron |
| CTAACGTGGTTAAAGGGGTT+GGG | 0.524400 | 2:-79090693 | MsG0280011069.01.T01:CDS |
| CCTCCACCACTGGTCTGCAA+TGG | 0.524420 | 2:+79090422 | None:intergenic |
| ATCACACAAAGCATTGATTG+AGG | 0.524445 | 2:-79090575 | MsG0280011069.01.T01:CDS |
| TGAAAATCTATCAATATTGG+AGG | 0.528441 | 2:+79090597 | None:intergenic |
| CCATTGCAGACCAGTGGTGG+AGG | 0.531905 | 2:-79090422 | MsG0280011069.01.T01:CDS |
| ATTCAGAAGCAAGGTAATGG+AGG | 0.545729 | 2:-79090484 | MsG0280011069.01.T01:CDS |
| CACCATGGTTCCAACGGACG+AGG | 0.546751 | 2:-79091067 | MsG0280011069.01.T01:CDS |
| ATCCTCGTCCGTTGGAACCA+TGG | 0.552840 | 2:+79091065 | None:intergenic |
| GCGACTTCTAAACAGAATCA+TGG | 0.566085 | 2:-79090643 | MsG0280011069.01.T01:CDS |
| CAATCACACCATGGTTCCAA+CGG | 0.579340 | 2:-79091073 | MsG0280011069.01.T01:CDS |
| GTGATGAAATCCTCGTCCGT+TGG | 0.582382 | 2:+79091057 | None:intergenic |
| CTACGCTAACGTGGTTAAAG+GGG | 0.595945 | 2:-79090698 | MsG0280011069.01.T01:CDS |
| TTACATCAAGCAGCCAAATG+AGG | 0.598629 | 2:-79090518 | MsG0280011069.01.T01:CDS |
| CCCTTTAACCACGTTAGCGT+AGG | 0.602141 | 2:+79090699 | None:intergenic |
| AGCCTTGTTAGATGAGGACG+AGG | 0.611957 | 2:+79090448 | None:intergenic |
| AAAGAAGAGATTCAGAAGCA+AGG | 0.613101 | 2:-79090493 | MsG0280011069.01.T01:CDS |
| AGACATTTGATGAGTTAACT+CGG | 0.619910 | 2:+79091035 | None:intergenic |
| TTCAGAAGCAAGGTAATGGA+GGG | 0.622585 | 2:-79090483 | MsG0280011069.01.T01:CDS |
| TTCGGAATTGTGACTCTGGG+AGG | 0.633765 | 2:+79090960 | None:intergenic |
| TTCCAACAAGGTGTAGCTGG+CGG | 0.645177 | 2:+79090730 | None:intergenic |
| TGGAGCAGCCTTGTTAGATG+AGG | 0.645334 | 2:+79090442 | None:intergenic |
| TGTAACCAGGGAGCTCAGAA+GGG | 0.649768 | 2:-79090771 | MsG0280011069.01.T01:intron |
| GTCCTCGTCCTCATCTAACA+AGG | 0.653193 | 2:-79090450 | MsG0280011069.01.T01:CDS |
| GCTGCTCCATTGCAGACCAG+TGG | 0.667639 | 2:-79090428 | MsG0280011069.01.T01:CDS |
| GATGATAATAATATGTACCA+TGG | 0.691410 | 2:-79091117 | None:intergenic |
| TAGCAACAACAATCACACCA+TGG | 0.737768 | 2:-79091082 | MsG0280011069.01.T01:CDS |
| TGCTAAGTGAGAGAACACCA+TGG | 0.818904 | 2:+79091100 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAAAGCCATTGAATTA+AGG | - | Chr2:79090973-79090992 | MsG0280011069.01.T01:intron | 20.0% |
| !! | TGATGAAAATCTATCAATAT+TGG | + | Chr2:79090931-79090950 | None:intergenic | 20.0% |
| !!! | ATGTTTTTTCCTTAATTCAA+TGG | + | Chr2:79090985-79091004 | None:intergenic | 20.0% |
| ! | CACACAAGAGTTAATAATAT+AGG | + | Chr2:79090659-79090678 | None:intergenic | 25.0% |
| ! | TGAAAATCTATCAATATTGG+AGG | + | Chr2:79090928-79090947 | None:intergenic | 25.0% |
| AGACATTTGATGAGTTAACT+CGG | + | Chr2:79090490-79090509 | None:intergenic | 30.0% | |
| GTTATTCAATAGACCTGAAA+AGG | + | Chr2:79090624-79090643 | None:intergenic | 30.0% | |
| AAAGAAGAGATTCAGAAGCA+AGG | - | Chr2:79091029-79091048 | MsG0280011069.01.T01:CDS | 35.0% | |
| ATCTCTTCTTTAGCCTCATT+TGG | + | Chr2:79091020-79091039 | None:intergenic | 35.0% | |
| GTGGGATTTAATTAGTTCAC+CGG | - | Chr2:79090705-79090724 | MsG0280011069.01.T01:CDS | 35.0% | |
| ! | TAGGTTTTTGTCTTCCAACA+AGG | + | Chr2:79090807-79090826 | None:intergenic | 35.0% |
| !! | ATCACACAAAGCATTGATTG+AGG | - | Chr2:79090947-79090966 | MsG0280011069.01.T01:intron | 35.0% |
| AATCCCACGACTACATGAAA+AGG | + | Chr2:79090693-79090712 | None:intergenic | 40.0% | |
| GAGATTCAGAAGCAAGGTAA+TGG | - | Chr2:79091035-79091054 | MsG0280011069.01.T01:CDS | 40.0% | |
| GCGACTTCTAAACAGAATCA+TGG | - | Chr2:79090879-79090898 | MsG0280011069.01.T01:intron | 40.0% | |
| TAGCAACAACAATCACACCA+TGG | - | Chr2:79090440-79090459 | MsG0280011069.01.T01:CDS | 40.0% | |
| TTACATCAAGCAGCCAAATG+AGG | - | Chr2:79091004-79091023 | MsG0280011069.01.T01:CDS | 40.0% | |
| ! | ATTCAGAAGCAAGGTAATGG+AGG | - | Chr2:79091038-79091057 | MsG0280011069.01.T01:CDS | 40.0% |
| ! | CTTCCTTTTCATGTAGTCGT+GGG | - | Chr2:79090687-79090706 | MsG0280011069.01.T01:CDS | 40.0% |
| ! | TAGTTCACCGGATTAACTTG+TGG | - | Chr2:79090717-79090736 | MsG0280011069.01.T01:CDS | 40.0% |
| ! | TCTTCCTTTTCATGTAGTCG+TGG | - | Chr2:79090686-79090705 | MsG0280011069.01.T01:CDS | 40.0% |
| ! | TGGATAGTAAGGGAAGCATA+TGG | + | Chr2:79090594-79090613 | None:intergenic | 40.0% |
| !! | TTCAGAAGCAAGGTAATGGA+GGG | - | Chr2:79091039-79091058 | MsG0280011069.01.T01:CDS | 40.0% |
| ACCGTGACCACAAGTTAATC+CGG | + | Chr2:79090727-79090746 | None:intergenic | 45.0% | |
| ACCTACGCTAACGTGGTTAA+AGG | - | Chr2:79090822-79090841 | MsG0280011069.01.T01:intron | 45.0% | |
| CAATCACACCATGGTTCCAA+CGG | - | Chr2:79090449-79090468 | MsG0280011069.01.T01:CDS | 45.0% | |
| CAGTTCGGAATTGTGACTCT+GGG | + | Chr2:79090568-79090587 | None:intergenic | 45.0% | |
| CCTACGCTAACGTGGTTAAA+GGG | - | Chr2:79090823-79090842 | MsG0280011069.01.T01:intron | 45.0% | |
| CTAACGTGGTTAAAGGGGTT+GGG | - | Chr2:79090829-79090848 | MsG0280011069.01.T01:intron | 45.0% | |
| CTACGCTAACGTGGTTAAAG+GGG | - | Chr2:79090824-79090843 | MsG0280011069.01.T01:intron | 45.0% | |
| GACAAAAACCTACGCTAACG+TGG | - | Chr2:79090815-79090834 | MsG0280011069.01.T01:intron | 45.0% | |
| TGCTAAGTGAGAGAACACCA+TGG | + | Chr2:79090425-79090444 | None:intergenic | 45.0% | |
| ! | GGAAGCATATGGTACCAGTT+CGG | + | Chr2:79090583-79090602 | None:intergenic | 45.0% |
| !! | ACCGGATTAACTTGTGGTCA+CGG | - | Chr2:79090723-79090742 | MsG0280011069.01.T01:CDS | 45.0% |
| CCAGAGTCACAATTCCGAAC+TGG | - | Chr2:79090566-79090585 | MsG0280011069.01.T01:CDS | 50.0% | |
| CCAGTTCGGAATTGTGACTC+TGG | + | Chr2:79090569-79090588 | None:intergenic | 50.0% | |
| CCCTTTAACCACGTTAGCGT+AGG | + | Chr2:79090826-79090845 | None:intergenic | 50.0% | |
| GCTAACGTGGTTAAAGGGGT+TGG | - | Chr2:79090828-79090847 | MsG0280011069.01.T01:intron | 50.0% | |
| GTCACGGTGAGTTGTAACCA+GGG | - | Chr2:79090739-79090758 | MsG0280011069.01.T01:CDS | 50.0% | |
| GTCCTCGTCCTCATCTAACA+AGG | - | Chr2:79091072-79091091 | MsG0280011069.01.T01:CDS | 50.0% | |
| GTCTTCCAACAAGGTGTAGC+TGG | + | Chr2:79090798-79090817 | None:intergenic | 50.0% | |
| GTGATGAAATCCTCGTCCGT+TGG | + | Chr2:79090468-79090487 | None:intergenic | 50.0% | |
| TGTAACCAGGGAGCTCAGAA+GGG | - | Chr2:79090751-79090770 | MsG0280011069.01.T01:CDS | 50.0% | |
| TTCCAACAAGGTGTAGCTGG+CGG | + | Chr2:79090795-79090814 | None:intergenic | 50.0% | |
| TTCGGAATTGTGACTCTGGG+AGG | + | Chr2:79090565-79090584 | None:intergenic | 50.0% | |
| TTGTAACCAGGGAGCTCAGA+AGG | - | Chr2:79090750-79090769 | MsG0280011069.01.T01:CDS | 50.0% | |
| ! | AAGGAAGCGCGTGGATAGTA+AGG | + | Chr2:79090605-79090624 | None:intergenic | 50.0% |
| ! | AGGAAGCGCGTGGATAGTAA+GGG | + | Chr2:79090604-79090623 | None:intergenic | 50.0% |
| ! | TATCCACGCGCTTCCTTTTC+AGG | - | Chr2:79090608-79090627 | MsG0280011069.01.T01:CDS | 50.0% |
| !! | AGCCTTGTTAGATGAGGACG+AGG | + | Chr2:79091077-79091096 | None:intergenic | 50.0% |
| !! | TGGAGCAGCCTTGTTAGATG+AGG | + | Chr2:79091083-79091102 | None:intergenic | 50.0% |
| ATCCTCGTCCGTTGGAACCA+TGG | + | Chr2:79090460-79090479 | None:intergenic | 55.0% | |
| GGTCACGGTGAGTTGTAACC+AGG | - | Chr2:79090738-79090757 | MsG0280011069.01.T01:CDS | 55.0% | |
| ! | AGACCTGAAAAGGAAGCGCG+TGG | + | Chr2:79090614-79090633 | None:intergenic | 55.0% |
| CACCATGGTTCCAACGGACG+AGG | - | Chr2:79090455-79090474 | MsG0280011069.01.T01:CDS | 60.0% | |
| CCATTGCAGACCAGTGGTGG+AGG | - | Chr2:79091100-79091119 | MsG0280011069.01.T01:CDS | 60.0% | |
| CTCCGCCAGCTACACCTTGT+TGG | - | Chr2:79090790-79090809 | MsG0280011069.01.T01:intron | 60.0% | |
| GATGACCCTTCTGAGCTCCC+TGG | + | Chr2:79090759-79090778 | None:intergenic | 60.0% | |
| GCTCCATTGCAGACCAGTGG+TGG | - | Chr2:79091097-79091116 | MsG0280011069.01.T01:CDS | 60.0% | |
| GCTGCTCCATTGCAGACCAG+TGG | - | Chr2:79091094-79091113 | MsG0280011069.01.T01:CDS | 60.0% | |
| ! | CCTCCACCACTGGTCTGCAA+TGG | + | Chr2:79091103-79091122 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 79090417 | 79091127 | 79090417 | ID=MsG0280011069.01;Name=MsG0280011069.01 |
| Chr2 | mRNA | 79090417 | 79091127 | 79090417 | ID=MsG0280011069.01.T01;Parent=MsG0280011069.01;Name=MsG0280011069.01.T01;_AED=0.49;_eAED=0.54;_QI=0|0|0|1|1|1|2|0|173 |
| Chr2 | exon | 79090974 | 79091127 | 79090974 | ID=MsG0280011069.01.T01:exon:37728;Parent=MsG0280011069.01.T01 |
| Chr2 | exon | 79090417 | 79090784 | 79090417 | ID=MsG0280011069.01.T01:exon:37729;Parent=MsG0280011069.01.T01 |
| Chr2 | CDS | 79090974 | 79091127 | 79090974 | ID=MsG0280011069.01.T01:cds;Parent=MsG0280011069.01.T01 |
| Chr2 | CDS | 79090417 | 79090784 | 79090417 | ID=MsG0280011069.01.T01:cds;Parent=MsG0280011069.01.T01 |
| Gene Sequence |
| Protein sequence |
