AlfalfaGEDB | MsG0280011306.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011306.01

KEGG: K09580
GO: GO:0045454
PFAM: PF00085

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011306.01.T01	XP_004486741.1	75.799	438	64	4	1	411	99	521	0	653

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011306.01.T01	Q66GQ3	50.569	439	174	5	1	411	111	534	2.03E-150	439

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011306.01.T01	G7II21	83.790	438	29	4	1	411	98	520	0.0	715

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280011306.01	MsG0480022090.01	0.825215	5.292754e-54	4.793973e-51
MsG0280011306.01	MsG0780039654.01	0.831195	1.915658e-55	2.063802e-52
MsG0280011306.01	MsG0780039657.01	0.812379	4.367877e-51	2.772764e-48
MsG0280011306.01	MsG0780039761.01	0.801744	7.793241e-49	3.750491e-46
MsG0280011306.01	MsG0880045022.01	0.809124	2.209188e-50	1.286291e-47
MsG0280011306.01	MsG0880047293.01	0.816759	4.685761e-52	3.349589e-49

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PPI

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Gene1	Gene2	Type
MsG0280010906.01	MsG0280011306.01	PPI
MsG0280011306.01	MsG0380016364.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011306.01.T01	MTR_2g101880	83.790	438	29	4	1	411	98	520	0.0	715
MsG0280011306.01.T01	MTR_2g093500	76.880	359	40	4	61	411	197	520	0.0	515
MsG0280011306.01.T01	MTR_2g094180	29.668	391	241	3	15	386	126	501	3.77e-60	205
MsG0280011306.01.T01	MTR_4g059450	31.552	393	231	5	16	387	149	524	2.04e-59	203
MsG0280011306.01.T01	MTR_3g088220	25.000	428	249	14	2	388	70	466	7.76e-23	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011306.01.T01	AT3G16110	50.569	439	174	5	1	411	111	534	2.07e-151	439
MsG0280011306.01.T01	AT1G52260	50.000	440	176	6	1	411	113	537	2.01e-146	427
MsG0280011306.01.T01	AT5G60640	29.298	413	256	4	2	395	140	535	3.68e-61	207
MsG0280011306.01.T01	AT5G60640	29.310	406	251	4	2	388	140	528	1.52e-60	206
MsG0280011306.01.T01	AT3G54960	28.148	405	253	6	2	386	136	522	2.66e-50	179
MsG0280011306.01.T01	AT3G54960	28.205	390	242	6	2	371	136	507	1.67e-46	167
MsG0280011306.01.T01	AT5G60640	28.316	392	209	8	2	388	140	464	2.94e-44	161
MsG0280011306.01.T01	AT1G21750	25.121	414	256	12	2	383	67	458	4.66e-24	104
MsG0280011306.01.T01	AT1G21750	25.121	414	256	12	2	383	67	458	4.69e-24	104
MsG0280011306.01.T01	AT1G77510	24.228	421	250	12	2	383	66	456	1.76e-21	96.7

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Find 87 sgRNAs with CRISPR-Local

Find 364 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGAGAGAATTATTAA+TGG	0.349452	2:+82183568	MsG0280011306.01.T01:five_prime_UTR
AAACAGAGAGAATTATTAAT+GGG	0.423977	2:+82183569	MsG0280011306.01.T01:five_prime_UTR
AACATGGGACCCTAAAATTT+AGG	0.332943	2:+82183189	MsG0280011306.01.T01:five_prime_UTR
AACCTGAAAGATATAACATA+TGG	0.486298	2:+82186207	MsG0280011306.01.T01:CDS
AACGAACTGGGTTTCATTAT+CGG	0.273450	2:-82186102	None:intergenic
AAGCAAATGATAAGACAAAT+CGG	0.498644	2:+82188730	MsG0280011306.01.T01:CDS
AAGCAAGCAAGTAGAGAAGT+TGG	0.475868	2:+82188465	MsG0280011306.01.T01:CDS
AAGCCCTTCCTGACAATGTT+TGG	0.312127	2:+82187137	MsG0280011306.01.T01:CDS
AAGGGTTAGAAGGAAATTGA+TGG	0.446480	2:-82183372	None:intergenic
AAGGTCACGGGTTCAAGTCC+TGG	0.449453	2:+82187987	MsG0280011306.01.T01:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAGAGAATGATTCTAAC+TGG	+	Chr2:82184541-82184560	MsG0280011306.01.T01:intron	30.0%
AACTGTTAGGACTTAGAATA+AGG	-	Chr2:82184909-82184928	None:intergenic	30.0%
AATATCTTTCTCGTACTAAC+AGG	+	Chr2:82184615-82184634	MsG0280011306.01.T01:intron	30.0%
ACAAATCGGTAATTACTCAA+TGG	+	Chr2:82188744-82188763	MsG0280011306.01.T01:intron	30.0%
ACATTTCAAGAACATCAGTT+GGG	+	Chr2:82187571-82187590	MsG0280011306.01.T01:intron	30.0%
ACCTCAACATATAAACATTC+AGG	-	Chr2:82184458-82184477	None:intergenic	30.0%
ACTAGTAGTAATAGAAACCA+TGG	-	Chr2:82183908-82183927	None:intergenic	30.0%
AGGAAGAAAAAAAATCATGG+CGG	-	Chr2:82186531-82186550	None:intergenic	30.0%
AGTCATTCACCTGAAAAATT+AGG	-	Chr2:82188689-82188708	None:intergenic	30.0%
ATTCAAATCTGCTCAGTATT+GGG	-	Chr2:82184520-82184539	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	82183170	82188937	82183170	ID=MsG0280011306.01;Name=MsG0280011306.01
Chr2	mRNA	82183170	82188937	82183170	ID=MsG0280011306.01.T01;Parent=MsG0280011306.01;Name=MsG0280011306.01.T01;_AED=0.42;_eAED=0.43;_QI=479\|0.58\|0.76\|0.84\|1\|1\|13\|0\|411
Chr2	exon	82183170	82183827	82183170	ID=MsG0280011306.01.T01:exon:12298;Parent=MsG0280011306.01.T01
Chr2	exon	82185781	82185910	82185781	ID=MsG0280011306.01.T01:exon:12299;Parent=MsG0280011306.01.T01
Chr2	exon	82186065	82186228	82186065	ID=MsG0280011306.01.T01:exon:12300;Parent=MsG0280011306.01.T01
Chr2	exon	82186789	82186852	82186789	ID=MsG0280011306.01.T01:exon:12301;Parent=MsG0280011306.01.T01
Chr2	exon	82186937	82187017	82186937	ID=MsG0280011306.01.T01:exon:12302;Parent=MsG0280011306.01.T01
Chr2	exon	82187095	82187184	82187095	ID=MsG0280011306.01.T01:exon:12303;Parent=MsG0280011306.01.T01
Chr2	exon	82187274	82187345	82187274	ID=MsG0280011306.01.T01:exon:12304;Parent=MsG0280011306.01.T01
Chr2	exon	82187430	82187498	82187430	ID=MsG0280011306.01.T01:exon:12305;Parent=MsG0280011306.01.T01
Chr2	exon	82187823	82187903	82187823	ID=MsG0280011306.01.T01:exon:12306;Parent=MsG0280011306.01.T01
Chr2	exon	82187990	82188034	82187990	ID=MsG0280011306.01.T01:exon:12307;Parent=MsG0280011306.01.T01
Chr2	exon	82188430	82188564	82188430	ID=MsG0280011306.01.T01:exon:12308;Parent=MsG0280011306.01.T01
Chr2	exon	82188699	82188751	82188699	ID=MsG0280011306.01.T01:exon:12309;Parent=MsG0280011306.01.T01
Chr2	exon	82188865	82188937	82188865	ID=MsG0280011306.01.T01:exon:12310;Parent=MsG0280011306.01.T01
Chr2	five_prime_UTR	82183170	82183648	82183170	ID=MsG0280011306.01.T01:five_prime_utr;Parent=MsG0280011306.01.T01
Chr2	CDS	82183649	82183827	82183649	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82185781	82185910	82185781	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82186065	82186228	82186065	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82186789	82186852	82186789	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82186937	82187017	82186937	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82187095	82187184	82187095	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82187274	82187345	82187274	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82187430	82187498	82187430	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82187823	82187903	82187823	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82187990	82188034	82187990	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82188430	82188564	82188430	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82188699	82188751	82188699	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01
Chr2	CDS	82188865	82188937	82188865	ID=MsG0280011306.01.T01:cds;Parent=MsG0280011306.01.T01

Gene Sequence

>MsG0280011306.01.T01

ATGCCTCATTTTGCTGAAGCTGCTAATTCTTTGAAAGATTTTGGAAATTCTCTTGTTTTGGCTAAGGTGGATGGTGATAGGTTTACTAAAGCTGCTTCTTTTCTTGGGATTAAAGGCTACCCTACTTTGCTGCTTTTTGTTAATGGGACTTCTCAACCTTATTCTGGCGGTTTCACTGCGGATGATATAGTTATATGGGCAAGAAAAAGGACCGGTACGCCTGTTATTCGGATAAGTACAGAGAAAGCAGCAGAGGAATTTCTGAAAAAGTATCACACTTTTCTCATTGGACGCTTTGATAAATTTGAGGGCCCTGAATATGAAGAATTTGTGAGTGCTGCAAAGTCCGATAATGAAACCCAGTTCGTTGAAGTGAGTAAGGTAGAGCTTGCCCAAGTTCTTTATCCAGATATCAAGCCTACTGGTAATTTTCTCGGGATCGTTAAGAGCGAACCTGAAAGATATAACATATGTTCTGTTCATATTCTGTCTCTTGCGTGTTTTTGCCACATGTTGATTGCCCCTGGATGCTTTGCTGTTTTTGTCTTTGCGAACATTGATGACTTCAAGAATCTTCTTGATCCACTTCAAGAAGTAGCAAGAACTTTCAAGTCGAAGATAATGTTTATATATGTGGATATTAACGACGAGAACCTTGCAAAGCCCTTCCTGACAATGTTTGGACTTGAAGAATCAACAAATACTGTGGTAGCTGCATTTGATAATGGAATGAGCTCAAAATTTCTATTGGAGTCAAAACCAACACGAAGCAATATTGAAGAGTTTTGCAGTAAACTTGTGCAAGGTTCTTTGTTGACTTACTTCAAGTCGCAGCCAATACCTGATAATACTGAAGCTAGTGTTCAAGTTATTGTTGGGAAAACATTTGATGAAGAAATTTTGAACAGCAAGAAGGATGTGGTTTTAGAGGTCACGGGTTCAAGTCCTGGAAACAGCCTCTTGTGTAAAAACAGGGTATTCACACCTTGGTGTTTCAACTGTGAGGACACAAGCAAGCAAGTAGAGAAGTTGGCAAAACACTACAAAAGTTCGAGTAATTTAATATTTGCAAAGATAGATGCTTCAGCAAATGAACATCCAAAACTGCAAGTGAATGACTTTCCCACACTTTTACTTTACAAAGCAAATGATAAGACAAATCGTTTGAAAGAATTGGCTGCATCCATCAACAAACACGTTAAAGTCAAGGATCAAGTCGCCAAAGATGAGCTATAG

Protein sequence

>MsG0280011306.01.T01

MPHFAEAANSLKDFGNSLVLAKVDGDRFTKAASFLGIKGYPTLLLFVNGTSQPYSGGFTADDIVIWARKRTGTPVIRISTEKAAEEFLKKYHTFLIGRFDKFEGPEYEEFVSAAKSDNETQFVEVSKVELAQVLYPDIKPTGNFLGIVKSEPERYNICSVHILSLACFCHMLIAPGCFAVFVFANIDDFKNLLDPLQEVARTFKSKIMFIYVDINDENLAKPFLTMFGLEESTNTVVAAFDNGMSSKFLLESKPTRSNIEEFCSKLVQGSLLTYFKSQPIPDNTEASVQVIVGKTFDEEILNSKKDVVLEVTGSSPGNSLLCKNRVFTPWCFNCEDTSKQVEKLAKHYKSSSNLIFAKIDASANEHPKLQVNDFPTLLLYKANDKTNRLKELAASINKHVKVKDQVAKDEL*