AlfalfaGEDB | MsG0280011379.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011379.01

KEGG: -
GO: -
PFAM: PF05553

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011379.01.T01	XP_003597867.1	95.833	96	4	0	1	96	1	96	1.74E-59	187
MsG0280011379.01.T02	XP_003597867.1	98.571	70	1	0	1	70	27	96	4.30E-42	142

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011379.01.T01	Q2HVV6	95.833	96	4	0	1	96	1	96	8.32e-60	187
MsG0280011379.01.T02	Q2HVV6	98.571	70	1	0	1	70	27	96	2.06e-42	142

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049008.01	MsG0280011379.01	0.806654	7.407084e-50	4.042344e-47
MsG0280011379.01	MsG0880045963.01	0.835522	1.598205e-56	1.959197e-53
MsG0280007667.01	MsG0280011379.01	0.802339	5.880582e-49	2.873423e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011379.01.T01	MTR_2g103440	95.833	96	4	0	1	96	1	96	2.11e-63	187
MsG0280011379.01.T01	MTR_4g011030	55.208	96	31	3	1	95	1	85	9.85e-23	84.0
MsG0280011379.01.T02	MTR_2g103440	98.571	70	1	0	1	70	27	96	5.21e-46	142
MsG0280011379.01.T02	MTR_4g011030	80.000	45	8	1	26	69	41	85	1.10e-19	75.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011379.01.T01	AT1G15385	57.778	45	16	1	51	95	31	72	5.19e-11	53.9
MsG0280011379.01.T02	AT1G15385	57.778	45	16	1	25	69	31	72	1.92e-11	53.9

Find 16 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCCTTCATCAAGAATTTC+AGG	0.206507	2:+83281139	MsG0280011379.01.T01:CDS
CATCTTCTCTCTCAAGTTGA+TGG	0.263758	2:-83280998	None:intergenic
TTTCCTGAAATTCTTGATGA+AGG	0.417879	2:-83281142	None:intergenic
CATTCTCTCATAATTCTCAA+TGG	0.446934	2:-83281190	None:intergenic
TAGCTTCTTATTTGTTTCAA+AGG	0.468483	2:-83281079	None:intergenic
ATCAGATCTCAAGAAAATAG+AGG	0.513230	2:+83280966	MsG0280011379.01.T01:CDS
CTTGATGAAGGCATCAGCAC+TGG	0.520908	2:-83281130	None:intergenic
GGAAACAACTCGTGATTCAA+AGG	0.528679	2:+83281160	MsG0280011379.01.T01:CDS
TCTTGAGATCTGATGATGGT+TGG	0.529007	2:-83280957	None:intergenic
AAAGTCATATCAACATGAGA+AGG	0.558128	2:+83280926	None:intergenic
AAACAAATAAGAAGCTAGAG+AGG	0.570340	2:+83281085	MsG0280011379.01.T01:CDS
ATTATGAGAGAATGCTTGCA+AGG	0.574381	2:+83281199	MsG0280011379.01.T01:CDS
TTATGAGAGAATGCTTGCAA+GGG	0.607940	2:+83281200	MsG0280011379.01.T01:CDS
ATTATCATATGATCATAATG+AGG	0.611197	2:+83281038	MsG0280011379.01.T01:CDS
TAATGAGGTAAAGAAAGTTG+AGG	0.652046	2:+83281053	MsG0280011379.01.T01:CDS
TATGAGAGAATGCTTGCAAG+GGG	0.671706	2:+83281201	MsG0280011379.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTATCATATGATCATAATG+AGG	+	Chr2:83281038-83281057	MsG0280011379.01.T01:CDS	20.0%
!!	TAGCTTCTTATTTGTTTCAA+AGG	-	Chr2:83281082-83281101	None:intergenic	25.0%
	AAACAAATAAGAAGCTAGAG+AGG	+	Chr2:83281085-83281104	MsG0280011379.01.T01:CDS	30.0%
	ATCAGATCTCAAGAAAATAG+AGG	+	Chr2:83280966-83280985	MsG0280011379.01.T01:CDS	30.0%
	CATTCTCTCATAATTCTCAA+TGG	-	Chr2:83281193-83281212	None:intergenic	30.0%
	TAATGAGGTAAAGAAAGTTG+AGG	+	Chr2:83281053-83281072	MsG0280011379.01.T01:CDS	30.0%
	TTTCCTGAAATTCTTGATGA+AGG	-	Chr2:83281145-83281164	None:intergenic	30.0%
!	ATTTTCTTGAGATCTGATGA+TGG	-	Chr2:83280964-83280983	None:intergenic	30.0%
	ATGCCTTCATCAAGAATTTC+AGG	+	Chr2:83281139-83281158	MsG0280011379.01.T01:CDS	35.0%
	ATTATGAGAGAATGCTTGCA+AGG	+	Chr2:83281199-83281218	MsG0280011379.01.T01:CDS	35.0%
	TTATGAGAGAATGCTTGCAA+GGG	+	Chr2:83281200-83281219	MsG0280011379.01.T01:CDS	35.0%
	GGAAACAACTCGTGATTCAA+AGG	+	Chr2:83281160-83281179	MsG0280011379.01.T01:CDS	40.0%
	TATGAGAGAATGCTTGCAAG+GGG	+	Chr2:83281201-83281220	MsG0280011379.01.T01:CDS	40.0%
!!	CATCTTCTCTCTCAAGTTGA+TGG	-	Chr2:83281001-83281020	None:intergenic	40.0%
!!	TCTTGAGATCTGATGATGGT+TGG	-	Chr2:83280960-83280979	None:intergenic	40.0%
!!	CTTGATGAAGGCATCAGCAC+TGG	-	Chr2:83281133-83281152	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	83280940	83281230	83280940	ID=MsG0280011379.01;Name=MsG0280011379.01
Chr2	mRNA	83280940	83281230	83280940	ID=MsG0280011379.01.T01;Parent=MsG0280011379.01;Name=MsG0280011379.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|96
Chr2	exon	83280940	83281230	83280940	ID=MsG0280011379.01.T01:exon:12438;Parent=MsG0280011379.01.T01
Chr2	CDS	83280940	83281230	83280940	ID=MsG0280011379.01.T01:cds;Parent=MsG0280011379.01.T01
Chr2	mRNA	83281018	83281230	83281018	ID=MsG0280011379.01.T02;Parent=MsG0280011379.01;Name=MsG0280011379.01.T02;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|70
Chr2	exon	83281018	83281230	83281018	ID=MsG0280011379.01.T02:exon:12439;Parent=MsG0280011379.01.T02
Chr2	CDS	83281018	83281230	83281018	ID=MsG0280011379.01.T02:cds;Parent=MsG0280011379.01.T02

Gene Sequence

>MsG0280011379.01.T01

ATGAGAAGGAGCTACATCCAACCATCATCAGATCTCAAGAAAATAGAGGAAAAAAGCACCATCAACTTGAGAGAGAAGATGAGAAACTCATCAACTTCATTATCATATGATCATAATGAGGTAAAGAAAGTTGAGGACTCCTTTGAAACAAATAAGAAGCTAGAGAGGAAGACTACAGAAGACATCAATGCCAGTGCTGATGCCTTCATCAAGAATTTCAGGAAACAACTCGTGATTCAAAGGCTTCAATCCATTGAGAATTATGAGAGAATGCTTGCAAGGGGTCTCTAG

>MsG0280011379.01.T02

ATGAGAAACTCATCAACTTCATTATCATATGATCATAATGAGGTAAAGAAAGTTGAGGACTCCTTTGAAACAAATAAGAAGCTAGAGAGGAAGACTACAGAAGACATCAATGCCAGTGCTGATGCCTTCATCAAGAATTTCAGGAAACAACTCGTGATTCAAAGGCTTCAATCCATTGAGAATTATGAGAGAATGCTTGCAAGGGGTCTCTAG

Protein sequence

>MsG0280011379.01.T01

MRRSYIQPSSDLKKIEEKSTINLREKMRNSSTSLSYDHNEVKKVEDSFETNKKLERKTTEDINASADAFIKNFRKQLVIQRLQSIENYERMLARGL*

>MsG0280011379.01.T02

MRNSSTSLSYDHNEVKKVEDSFETNKKLERKTTEDINASADAFIKNFRKQLVIQRLQSIENYERMLARGL*