AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049008.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049008.01.T01 MTR_8g104765 97.222 216 6 0 1 216 1 216 4.14e-158 436
MsG0080049008.01.T01 MTR_4g120830 93.056 216 6 1 1 216 1 207 1.17e-147 409
MsG0080049008.01.T01 MTR_4g120825 92.593 216 7 1 1 216 1 207 6.13e-147 407
MsG0080049008.01.T01 MTR_3g008840 39.888 178 95 4 38 213 19 186 4.43e-38 130
MsG0080049008.01.T01 MTR_4g101760 34.951 206 118 5 9 213 11 201 6.80e-37 128
MsG0080049008.01.T01 MTR_4g035905 40.588 170 87 4 44 211 36 193 9.07e-37 127
MsG0080049008.01.T01 MTR_5g012000 40.351 171 93 4 42 211 46 208 6.30e-36 126
MsG0080049008.01.T01 MTR_4g035880 40.237 169 89 3 44 211 36 193 1.57e-35 124
MsG0080049008.01.T01 MTR_4g035870 37.688 199 107 5 11 208 8 190 6.24e-35 123
MsG0080049008.01.T01 MTR_3g008820 36.932 176 99 4 38 211 18 183 6.60e-34 120
MsG0080049008.01.T01 MTR_4g025610 34.884 172 107 2 44 213 32 200 5.94e-33 118
MsG0080049008.01.T01 MTR_7g108790 34.884 172 100 3 45 216 34 193 1.58e-32 117
MsG0080049008.01.T01 MTR_4g025550 30.952 210 132 4 6 213 2 200 1.32e-31 115
MsG0080049008.01.T01 MTR_4g025590 34.302 172 108 2 44 213 3 171 1.38e-31 114
MsG0080049008.01.T01 MTR_3g008760 33.333 183 113 2 28 209 50 224 7.66e-31 113
MsG0080049008.01.T01 MTR_4g025620 33.721 172 109 2 44 213 32 200 2.08e-30 112
MsG0080049008.01.T01 MTR_2g043560 36.994 173 89 4 43 212 33 188 6.65e-28 105
MsG0080049008.01.T01 MTR_1g088540 37.419 155 85 3 61 215 3 145 2.32e-26 99.8
MsG0080049008.01.T01 MTR_4g035855 31.925 213 132 6 1 211 1 202 4.25e-26 100
MsG0080049008.01.T01 MTR_4g124855 31.884 207 113 9 7 208 11 194 4.35e-25 97.8
MsG0080049008.01.T01 MTR_0036s0140 32.317 164 90 6 45 207 78 221 7.66e-17 76.6
MsG0080049008.01.T01 MTR_3g010780 29.697 165 101 4 45 207 71 222 4.21e-16 76.6
MsG0080049008.01.T01 MTR_8g042900 29.518 166 101 4 45 207 76 228 3.94e-15 73.9
MsG0080049008.01.T01 MTR_8g023150 25.150 167 108 3 45 208 77 229 7.75e-14 70.1
MsG0080049008.01.T01 MTR_8g023310 25.581 172 117 3 44 213 67 229 4.19e-13 68.2
MsG0080049008.01.T01 MTR_3g091380 25.000 200 140 4 9 206 42 233 2.06e-12 65.9
MsG0080049008.01.T01 MTR_1g008140 26.506 166 113 4 45 206 82 242 2.60e-12 65.9
MsG0080049008.01.T01 MTR_7g112580 24.623 199 137 4 10 206 3 190 7.85e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049008.01.T01 AT2G01610 56.842 190 76 4 33 216 33 222 2.34e-70 214
MsG0080049008.01.T01 AT1G14890 54.106 207 77 5 9 214 29 218 1.23e-68 209
MsG0080049008.01.T01 AT1G23205 53.846 182 74 3 39 215 28 204 6.30e-60 187
MsG0080049008.01.T01 AT1G70720 44.860 214 106 4 3 215 8 210 1.67e-57 181
MsG0080049008.01.T01 AT1G70720 47.959 196 90 4 21 215 14 198 1.35e-56 178
MsG0080049008.01.T01 AT5G62350 40.642 187 103 3 27 212 21 200 5.62e-40 136
MsG0080049008.01.T01 AT4G25260 37.745 204 115 5 12 213 7 200 3.69e-39 134
MsG0080049008.01.T01 AT3G47380 39.153 189 105 4 28 213 20 201 2.39e-37 129
MsG0080049008.01.T01 AT4G12390 41.520 171 93 3 44 213 39 203 5.08e-36 126
MsG0080049008.01.T01 AT1G62770 37.968 187 106 5 29 213 20 198 5.30e-35 123
MsG0080049008.01.T01 AT4G25250 32.020 203 127 5 9 210 4 196 1.22e-33 119
MsG0080049008.01.T01 AT5G20740 36.019 211 114 6 10 216 7 200 9.02e-33 117
MsG0080049008.01.T01 AT5G20740 35.096 208 120 5 10 216 41 234 3.10e-32 117
MsG0080049008.01.T01 AT1G62760 37.209 172 97 4 41 211 144 305 3.94e-31 116
MsG0080049008.01.T01 AT5G51520 31.220 205 121 5 10 207 8 199 5.10e-31 113
MsG0080049008.01.T01 AT5G62360 35.294 170 97 4 43 211 42 199 7.54e-31 112
MsG0080049008.01.T01 AT4G00080 30.542 203 122 5 9 207 9 196 9.58e-26 99.8
MsG0080049008.01.T01 AT2G47670 32.370 173 104 4 44 215 48 208 7.48e-21 87.0
MsG0080049008.01.T01 AT3G62820 26.829 205 126 5 13 215 12 194 2.42e-20 85.1
MsG0080049008.01.T01 AT3G14300 27.059 170 108 4 45 207 271 431 1.01e-15 75.9
MsG0080049008.01.T01 AT3G14300 26.437 174 118 5 42 213 456 621 1.90e-11 63.2
MsG0080049008.01.T01 AT5G53370 26.066 211 135 7 10 216 78 271 1.10e-14 72.8
MsG0080049008.01.T01 AT5G53370 26.066 211 135 7 10 216 42 235 1.26e-14 72.4
MsG0080049008.01.T01 AT3G49220 28.293 205 115 4 10 206 97 277 4.52e-14 70.9
MsG0080049008.01.T01 AT3G49220 28.293 205 115 4 10 206 52 232 4.73e-14 70.9
MsG0080049008.01.T01 AT3G49220 28.293 205 115 4 10 206 97 277 5.37e-14 70.5
MsG0080049008.01.T01 AT5G62340 27.232 224 133 10 1 213 1 205 2.57e-13 66.6
MsG0080049008.01.T01 AT1G53830 25.301 166 112 4 44 207 61 216 6.57e-11 61.6

Find 60 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGCTTTGGCACTTGTTAA+TGG 0.258713 contig604end:+28266 MsG0080049008.01.T01:CDS
GTTAATGGTTACGCACAAAA+AGG 0.286538 contig604end:+28281 MsG0080049008.01.T01:CDS
GCAACTTGAACGGATGAATT+CGG 0.350009 contig604end:-27787 None:intergenic
TCTGCTCCGGTGGCGGTTGT+TGG 0.357625 contig604end:-27738 None:intergenic
CGAAGAAGATGATTGATAAT+AGG 0.374294 contig604end:-27692 None:intergenic
GTCGGAACTGGCGCCGGTGC+CGG 0.392219 contig604end:+28083 MsG0080049008.01.T01:CDS
ATCTGCTTCGCGAGTGAGAT+TGG 0.399064 contig604end:-27949 None:intergenic
CAGATTGGAGCCGTCGGAAC+TGG 0.401781 contig604end:+28071 MsG0080049008.01.T01:CDS
GGCGGTTGTTGGACGGAGAT+TGG 0.404587 contig604end:-27727 None:intergenic
AGGGAAGTGTAGCATATGTC+AGG 0.436656 contig604end:-27825 None:intergenic
GTGGACTTTGGAGAAGCTTA+CGG 0.443650 contig604end:-27907 None:intergenic
AAAGTTCACGAGTAATGCTT+TGG 0.448902 contig604end:+28253 MsG0080049008.01.T01:CDS
TTTGGAGAAGCTTACGGCTA+TGG 0.464721 contig604end:-27901 None:intergenic
GGGATTCTGTTGAACTGCAT+TGG 0.465344 contig604end:-27859 None:intergenic
AGAGGCGGTTCGGTGGACTT+TGG 0.468926 contig604end:-27919 None:intergenic
CTTAAACAGATGCGACAGAT+TGG 0.471352 contig604end:+28056 MsG0080049008.01.T01:CDS
CTCCGGTGGCGGTTGTTGGA+CGG 0.475048 contig604end:-27734 None:intergenic
GGCGATGCGAGCGAGTTGGC+CGG 0.484500 contig604end:-27880 None:intergenic
GCGTCGTCGTTGGTGGTGGT+TGG 0.487249 contig604end:-27765 None:intergenic
GAGGAAACTGTGAGAGTGTT+GGG 0.489323 contig604end:-27670 None:intergenic
TGGAGGTTCTGCTCCGGTGG+CGG 0.492157 contig604end:-27745 None:intergenic
GGAGGAAACTGTGAGAGTGT+TGG 0.492920 contig604end:-27671 None:intergenic
AAACGAATCTGCTCCGGCAC+CGG 0.499792 contig604end:-28096 None:intergenic
TTCTGTACATCCGCTTTCAC+AGG 0.501048 contig604end:-28212 None:intergenic
TCGTCGTTGGTGGTGGTTGG+AGG 0.502344 contig604end:-27762 None:intergenic
CTCCGTCCAACAACCGCCAC+CGG 0.510383 contig604end:+27732 MsG0080049008.01.T01:CDS
TGGAGAGATAAGAGGCGGTT+CGG 0.511261 contig604end:-27929 None:intergenic
GGTGGTTGGAGGTTCTGCTC+CGG 0.513292 contig604end:-27751 None:intergenic
GATGAATTCGGCGTCGTCGT+TGG 0.515433 contig604end:-27775 None:intergenic
TTCGGCGTCGTCGTTGGTGG+TGG 0.522970 contig604end:-27769 None:intergenic
AGTGAGATTGGAGAGATAAG+AGG 0.523775 contig604end:-27937 None:intergenic
GGCACCGGCGCCAGTTCCGA+CGG 0.530037 contig604end:-28081 None:intergenic
CTGCATTGGCGTAGCGAGAG+AGG 0.532228 contig604end:-27845 None:intergenic
AGAAGATGATTGATAATAGG+AGG 0.540415 contig604end:-27689 None:intergenic
GAATTCGGCGTCGTCGTTGG+TGG 0.541966 contig604end:-27772 None:intergenic
AAACAGAAACGAATCTGCTC+CGG 0.547260 contig604end:-28102 None:intergenic
GGTTGGAGGTTCTGCTCCGG+TGG 0.548791 contig604end:-27748 None:intergenic
GCGATGCGAGCGAGTTGGCC+GGG 0.559441 contig604end:-27879 None:intergenic
GGAGCCGTCGGAACTGGCGC+CGG 0.568149 contig604end:+28077 MsG0080049008.01.T01:CDS
TGCATTGGCGTAGCGAGAGA+GGG 0.576173 contig604end:-27844 None:intergenic
TGTACAGAATCGTGTCTCGA+AGG 0.577511 contig604end:+28226 MsG0080049008.01.T01:CDS
CTATGGCGATGCGAGCGAGT+TGG 0.577700 contig604end:-27884 None:intergenic
TACTGATGAAGAAACGTGTA+CGG 0.583108 contig604end:+28163 MsG0080049008.01.T01:CDS
CGAGATTTGAGAAACAGTCA+TGG 0.583249 contig604end:-28004 None:intergenic
GATGAAGAAACGTGTACGGA+TGG 0.588482 contig604end:+28167 MsG0080049008.01.T01:CDS
ACAGTCATGGAGAGCGAGTG+CGG 0.590149 contig604end:-27991 None:intergenic
AATGCAGTTCAACAGAATCC+CGG 0.598872 contig604end:+27861 MsG0080049008.01.T01:CDS
AGATGAGTAACGTGCAGACG+TGG 0.606703 contig604end:+28126 MsG0080049008.01.T01:CDS
AGAGATAAGAGGCGGTTCGG+TGG 0.627950 contig604end:-27926 None:intergenic
GGGAAGTGTAGCATATGTCA+GGG 0.632502 contig604end:-27824 None:intergenic
GCATCTGTTTAAGCGAGCCA+CGG 0.637289 contig604end:-28046 None:intergenic
GTGTTGCGTTGCAACTTGAA+CGG 0.641224 contig604end:-27797 None:intergenic
ATGCGACAGATTGGAGCCGT+CGG 0.644503 contig604end:+28065 MsG0080049008.01.T01:CDS
TGGACGGAGATTGGAGAATG+TGG 0.645484 contig604end:-27718 None:intergenic
CGTGCAGACGTGGATGAGTG+CGG 0.674734 contig604end:+28136 MsG0080049008.01.T01:CDS
GAGATTGGAGAGATAAGAGG+CGG 0.675120 contig604end:-27934 None:intergenic
AGAAGATTGTCCTGTGAAAG+CGG 0.686477 contig604end:+28202 MsG0080049008.01.T01:CDS
ACGGAGATTGGAGAATGTGG+CGG 0.690066 contig604end:-27715 None:intergenic
GATGCTGTTGATGAAATCCG+TGG 0.736309 contig604end:+28029 MsG0080049008.01.T01:CDS
ACTCGCGAAGCAGATTACAG+CGG 0.792244 contig604end:+27957 MsG0080049008.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAGATGATTGATAATAGG+AGG - contig604end:27692-27711 None:intergenic 30.0%
! CGAAGAAGATGATTGATAAT+AGG - contig604end:27695-27714 None:intergenic 30.0%
AAAGTTCACGAGTAATGCTT+TGG + contig604end:28253-28272 MsG0080049008.01.T01:CDS 35.0%
GTTAATGGTTACGCACAAAA+AGG + contig604end:28281-28300 MsG0080049008.01.T01:CDS 35.0%
TACTGATGAAGAAACGTGTA+CGG + contig604end:28163-28182 MsG0080049008.01.T01:CDS 35.0%
! AATGCTTTGGCACTTGTTAA+TGG + contig604end:28266-28285 MsG0080049008.01.T01:CDS 35.0%
AAACAGAAACGAATCTGCTC+CGG - contig604end:28105-28124 None:intergenic 40.0%
AATGCAGTTCAACAGAATCC+CGG + contig604end:27861-27880 MsG0080049008.01.T01:CDS 40.0%
AGAAGATTGTCCTGTGAAAG+CGG + contig604end:28202-28221 MsG0080049008.01.T01:CDS 40.0%
AGTGAGATTGGAGAGATAAG+AGG - contig604end:27940-27959 None:intergenic 40.0%
CGAGATTTGAGAAACAGTCA+TGG - contig604end:28007-28026 None:intergenic 40.0%
CTTAAACAGATGCGACAGAT+TGG + contig604end:28056-28075 MsG0080049008.01.T01:CDS 40.0%
GCAACTTGAACGGATGAATT+CGG - contig604end:27790-27809 None:intergenic 40.0%
GAGATTGGAGAGATAAGAGG+CGG - contig604end:27937-27956 None:intergenic 45.0%
GATGAAGAAACGTGTACGGA+TGG + contig604end:28167-28186 MsG0080049008.01.T01:CDS 45.0%
GGGATTCTGTTGAACTGCAT+TGG - contig604end:27862-27881 None:intergenic 45.0%
GTGTTGCGTTGCAACTTGAA+CGG - contig604end:27800-27819 None:intergenic 45.0%
TGTACAGAATCGTGTCTCGA+AGG + contig604end:28226-28245 MsG0080049008.01.T01:CDS 45.0%
TTCTGTACATCCGCTTTCAC+AGG - contig604end:28215-28234 None:intergenic 45.0%
TTTGGAGAAGCTTACGGCTA+TGG - contig604end:27904-27923 None:intergenic 45.0%
! AGGGAAGTGTAGCATATGTC+AGG - contig604end:27828-27847 None:intergenic 45.0%
! GATGCTGTTGATGAAATCCG+TGG + contig604end:28029-28048 MsG0080049008.01.T01:CDS 45.0%
! GGGAAGTGTAGCATATGTCA+GGG - contig604end:27827-27846 None:intergenic 45.0%
! GTGGACTTTGGAGAAGCTTA+CGG - contig604end:27910-27929 None:intergenic 45.0%
!! GAGGAAACTGTGAGAGTGTT+GGG - contig604end:27673-27692 None:intergenic 45.0%
ACGGAGATTGGAGAATGTGG+CGG - contig604end:27718-27737 None:intergenic 50.0%
ACTCGCGAAGCAGATTACAG+CGG + contig604end:27957-27976 MsG0080049008.01.T01:CDS 50.0%
AGATGAGTAACGTGCAGACG+TGG + contig604end:28126-28145 MsG0080049008.01.T01:CDS 50.0%
ATCTGCTTCGCGAGTGAGAT+TGG - contig604end:27952-27971 None:intergenic 50.0%
GCATCTGTTTAAGCGAGCCA+CGG - contig604end:28049-28068 None:intergenic 50.0%
TGGACGGAGATTGGAGAATG+TGG - contig604end:27721-27740 None:intergenic 50.0%
TGGAGAGATAAGAGGCGGTT+CGG - contig604end:27932-27951 None:intergenic 50.0%
! GGAGGAAACTGTGAGAGTGT+TGG - contig604end:27674-27693 None:intergenic 50.0%
AAACGAATCTGCTCCGGCAC+CGG - contig604end:28099-28118 None:intergenic 55.0%
ACAGTCATGGAGAGCGAGTG+CGG - contig604end:27994-28013 None:intergenic 55.0%
ATGCGACAGATTGGAGCCGT+CGG + contig604end:28065-28084 MsG0080049008.01.T01:CDS 55.0%
GATGAATTCGGCGTCGTCGT+TGG - contig604end:27778-27797 None:intergenic 55.0%
! AGAGATAAGAGGCGGTTCGG+TGG - contig604end:27929-27948 None:intergenic 55.0%
! TGCATTGGCGTAGCGAGAGA+GGG - contig604end:27847-27866 None:intergenic 55.0%
CAGATTGGAGCCGTCGGAAC+TGG + contig604end:28071-28090 MsG0080049008.01.T01:CDS 60.0%
CGTGCAGACGTGGATGAGTG+CGG + contig604end:28136-28155 MsG0080049008.01.T01:CDS 60.0%
CTATGGCGATGCGAGCGAGT+TGG - contig604end:27887-27906 None:intergenic 60.0%
GAATTCGGCGTCGTCGTTGG+TGG - contig604end:27775-27794 None:intergenic 60.0%
GGCGGTTGTTGGACGGAGAT+TGG - contig604end:27730-27749 None:intergenic 60.0%
GGTGGTTGGAGGTTCTGCTC+CGG - contig604end:27754-27773 None:intergenic 60.0%
! CTGCATTGGCGTAGCGAGAG+AGG - contig604end:27848-27867 None:intergenic 60.0%
!! AGAGGCGGTTCGGTGGACTT+TGG - contig604end:27922-27941 None:intergenic 60.0%
!! TCGTCGTTGGTGGTGGTTGG+AGG - contig604end:27765-27784 None:intergenic 60.0%
CTCCGTCCAACAACCGCCAC+CGG + contig604end:27732-27751 MsG0080049008.01.T01:CDS 65.0%
GGTTGGAGGTTCTGCTCCGG+TGG - contig604end:27751-27770 None:intergenic 65.0%
TGGAGGTTCTGCTCCGGTGG+CGG - contig604end:27748-27767 None:intergenic 65.0%
! CTCCGGTGGCGGTTGTTGGA+CGG - contig604end:27737-27756 None:intergenic 65.0%
! TCTGCTCCGGTGGCGGTTGT+TGG - contig604end:27741-27760 None:intergenic 65.0%
! TTCGGCGTCGTCGTTGGTGG+TGG - contig604end:27772-27791 None:intergenic 65.0%
!! GCGTCGTCGTTGGTGGTGGT+TGG - contig604end:27768-27787 None:intergenic 65.0%
! GCGATGCGAGCGAGTTGGCC+GGG - contig604end:27882-27901 None:intergenic 70.0%
! GGCGATGCGAGCGAGTTGGC+CGG - contig604end:27883-27902 None:intergenic 70.0%
GGAGCCGTCGGAACTGGCGC+CGG + contig604end:28077-28096 MsG0080049008.01.T01:CDS 75.0%
!! GGCACCGGCGCCAGTTCCGA+CGG - contig604end:28084-28103 None:intergenic 75.0%
!! GTCGGAACTGGCGCCGGTGC+CGG + contig604end:28083-28102 MsG0080049008.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
contig604end gene 27663 28313 27663 ID=MsG0080049008.01;Name=MsG0080049008.01
contig604end mRNA 27663 28313 27663 ID=MsG0080049008.01.T01;Parent=MsG0080049008.01;Name=MsG0080049008.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|216
contig604end exon 27663 28313 27663 ID=MsG0080049008.01.T01:exon:11009;Parent=MsG0080049008.01.T01
contig604end CDS 27663 28313 27663 ID=MsG0080049008.01.T01:cds;Parent=MsG0080049008.01.T01
Gene Sequence

>MsG0080049008.01.T01

ATGACAACCCAACACTCTCACAGTTTCCTCCTATTATCAATCATCTTCTTCGCCGCCACATTCTCCAATCTCCGTCCAACAACCGCCACCGGAGCAGAACCTCCAACCACCACCAACGACGACGCCGAATTCATCCGTTCAAGTTGCAACGCAACACTCTACCCTGACATATGCTACACTTCCCTCTCTCGCTACGCCAATGCAGTTCAACAGAATCCCGGCCAACTCGCTCGCATCGCCATAGCCGTAAGCTTCTCCAAAGTCCACCGAACCGCCTCTTATCTCTCCAATCTCACTCGCGAAGCAGATTACAGCGGTAGTTCACGCGCCGCACTCGCTCTCCATGACTGTTTCTCAAATCTCGACGATGCTGTTGATGAAATCCGTGGCTCGCTTAAACAGATGCGACAGATTGGAGCCGTCGGAACTGGCGCCGGTGCCGGAGCAGATTCGTTTCTGTTTCAGATGAGTAACGTGCAGACGTGGATGAGTGCGGCACTTACTGATGAAGAAACGTGTACGGATGGATTTCAAGATGTAGAAGATTGTCCTGTGAAAGCGGATGTACAGAATCGTGTCTCGAAGGTGAAAAAGTTCACGAGTAATGCTTTGGCACTTGTTAATGGTTACGCACAAAAAGGAATGCCATGA

Protein sequence

>MsG0080049008.01.T01

MTTQHSHSFLLLSIIFFAATFSNLRPTTATGAEPPTTTNDDAEFIRSSCNATLYPDICYTSLSRYANAVQQNPGQLARIAIAVSFSKVHRTASYLSNLTREADYSGSSRAALALHDCFSNLDDAVDEIRGSLKQMRQIGAVGTGAGAGADSFLFQMSNVQTWMSAALTDEETCTDGFQDVEDCPVKADVQNRVSKVKKFTSNALALVNGYAQKGMP*