Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011418.01.T01 | PNX75896.1 | 83.871 | 62 | 10 | 0 | 1 | 62 | 1 | 62 | 1.76E-28 | 106 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011418.01.T01 | O80358 | 66.667 | 60 | 20 | 0 | 1 | 60 | 1 | 60 | 1.08E-23 | 94.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011418.01.T01 | A0A2K3LBI8 | 83.871 | 62 | 10 | 0 | 1 | 62 | 1 | 62 | 8.40e-29 | 106 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003786.01 | MsG0280011418.01 | 0.800838 | 1.194563e-48 | 5.619005e-46 |
| MsG0280011418.01 | MsG0780038603.01 | 0.804875 | 1.750718e-49 | 9.130193e-47 |
| MsG0280007452.01 | MsG0280011418.01 | 0.803997 | 2.668486e-49 | 1.360444e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011418.01.T01 | MTR_2g104100 | 93.333 | 60 | 4 | 0 | 1 | 60 | 1 | 60 | 3.31e-32 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011418.01.T01 | AT1G52500 | 66.667 | 60 | 20 | 0 | 1 | 60 | 1 | 60 | 9.73e-26 | 94.4 |
| MsG0280011418.01.T01 | AT1G52500 | 66.667 | 60 | 20 | 0 | 1 | 60 | 1 | 60 | 1.10e-24 | 94.4 |
| MsG0280011418.01.T01 | AT1G52500 | 66.667 | 60 | 20 | 0 | 1 | 60 | 1 | 60 | 3.00e-24 | 91.7 |
| MsG0280011418.01.T01 | AT1G52500 | 66.667 | 60 | 20 | 0 | 1 | 60 | 1 | 60 | 3.00e-24 | 91.7 |
| MsG0280011418.01.T01 | AT1G52500 | 66.667 | 60 | 20 | 0 | 1 | 60 | 1 | 60 | 1.07e-23 | 90.1 |
Find 20 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGTTCGAAGCTTCCGTTGT+TGG | 0.390645 | 2:-83700303 | MsG0280011418.01.T01:CDS |
| ATCATCGGCGACGATGCATT+TGG | 0.423442 | 2:+83700356 | None:intergenic |
| AGTTCGAAGCTTCCGTTGTT+GGG | 0.447631 | 2:-83700302 | MsG0280011418.01.T01:CDS |
| GCCGGAGCTACCGGAGGTAG+AGG | 0.468617 | 2:-83700424 | MsG0280011418.01.T01:CDS |
| TTGTTGCAGCTCGTCAGAAA+GGG | 0.472253 | 2:-83700272 | MsG0280011418.01.T01:CDS |
| GAGCACAATGCCGGAGCTAC+CGG | 0.482856 | 2:-83700433 | None:intergenic |
| GCCTCTACCTCCGGTAGCTC+CGG | 0.498178 | 2:+83700423 | None:intergenic |
| ATTGTTGCAGCTCGTCAGAA+AGG | 0.513195 | 2:-83700273 | MsG0280011418.01.T01:CDS |
| CTTCTCGCCGCCTCTACCTC+CGG | 0.514260 | 2:+83700414 | None:intergenic |
| CGGTGGAAGAGAACTGTATC+GGG | 0.530121 | 2:-83700389 | MsG0280011418.01.T01:CDS |
| GCGGTGGAAGAGAACTGTAT+CGG | 0.546118 | 2:-83700390 | MsG0280011418.01.T01:CDS |
| GTCGATGACTTTGTCATCAT+CGG | 0.552732 | 2:+83700341 | None:intergenic |
| AGAGGCGGCGAGAAGAGCGG+TGG | 0.561155 | 2:-83700406 | MsG0280011418.01.T01:CDS |
| TCAGAAAGGGGAAGAATATG+TGG | 0.584479 | 2:-83700259 | MsG0280011418.01.T01:CDS |
| GGTAGAGGCGGCGAGAAGAG+CGG | 0.609449 | 2:-83700409 | MsG0280011418.01.T01:CDS |
| TGTTGCAGCTCGTCAGAAAG+GGG | 0.642951 | 2:-83700271 | MsG0280011418.01.T01:CDS |
| GGAGCTACCGGAGGTAGAGG+CGG | 0.644715 | 2:-83700421 | MsG0280011418.01.T01:CDS |
| CACAATGCCGGAGCTACCGG+AGG | 0.654051 | 2:-83700430 | None:intergenic |
| AACAATCGTCTTCCCAACAA+CGG | 0.658793 | 2:+83700290 | None:intergenic |
| GATGATGACAAAGTCATCGA+CGG | 0.687773 | 2:-83700339 | MsG0280011418.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAATCGTCTTCCCAACAA+CGG | + | Chr2:83700387-83700406 | None:intergenic | 40.0% | |
| GATGATGACAAAGTCATCGA+CGG | - | Chr2:83700335-83700354 | MsG0280011418.01.T01:CDS | 40.0% | |
| GTCGATGACTTTGTCATCAT+CGG | + | Chr2:83700336-83700355 | None:intergenic | 40.0% | |
| TCAGAAAGGGGAAGAATATG+TGG | - | Chr2:83700415-83700434 | MsG0280011418.01.T01:CDS | 40.0% | |
| AGTTCGAAGCTTCCGTTGTT+GGG | - | Chr2:83700372-83700391 | MsG0280011418.01.T01:CDS | 45.0% | |
| ATTGTTGCAGCTCGTCAGAA+AGG | - | Chr2:83700401-83700420 | MsG0280011418.01.T01:CDS | 45.0% | |
| TTGTTGCAGCTCGTCAGAAA+GGG | - | Chr2:83700402-83700421 | MsG0280011418.01.T01:CDS | 45.0% | |
| ATCATCGGCGACGATGCATT+TGG | + | Chr2:83700321-83700340 | None:intergenic | 50.0% | |
| CGGTGGAAGAGAACTGTATC+GGG | - | Chr2:83700285-83700304 | MsG0280011418.01.T01:CDS | 50.0% | |
| GAGTTCGAAGCTTCCGTTGT+TGG | - | Chr2:83700371-83700390 | MsG0280011418.01.T01:CDS | 50.0% | |
| GCGGTGGAAGAGAACTGTAT+CGG | - | Chr2:83700284-83700303 | MsG0280011418.01.T01:CDS | 50.0% | |
| TGTTGCAGCTCGTCAGAAAG+GGG | - | Chr2:83700403-83700422 | MsG0280011418.01.T01:CDS | 50.0% | |
| CTTCTCGCCGCCTCTACCTC+CGG | + | Chr2:83700263-83700282 | None:intergenic | 65.0% | |
| GCCTCTACCTCCGGTAGCTC+CGG | + | Chr2:83700254-83700273 | None:intergenic | 65.0% | |
| GGAGCTACCGGAGGTAGAGG+CGG | - | Chr2:83700253-83700272 | MsG0280011418.01.T01:CDS | 65.0% | |
| GGTAGAGGCGGCGAGAAGAG+CGG | - | Chr2:83700265-83700284 | MsG0280011418.01.T01:CDS | 65.0% | |
| AGAGGCGGCGAGAAGAGCGG+TGG | - | Chr2:83700268-83700287 | MsG0280011418.01.T01:CDS | 70.0% | |
| GCCGGAGCTACCGGAGGTAG+AGG | - | Chr2:83700250-83700269 | MsG0280011418.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 83700248 | 83700448 | 83700248 | ID=MsG0280011418.01;Name=MsG0280011418.01 |
| Chr2 | mRNA | 83700248 | 83700448 | 83700248 | ID=MsG0280011418.01.T01;Parent=MsG0280011418.01;Name=MsG0280011418.01.T01;_AED=0.49;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|66 |
| Chr2 | exon | 83700248 | 83700448 | 83700248 | ID=MsG0280011418.01.T01:exon:3158;Parent=MsG0280011418.01.T01 |
| Chr2 | CDS | 83700248 | 83700448 | 83700248 | ID=MsG0280011418.01.T01:cds;Parent=MsG0280011418.01.T01 |
| Gene Sequence |
| Protein sequence |
