Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011676.01.T01 | XP_003598217.2 | 99.02 | 102 | 1 | 0 | 1 | 102 | 2 | 103 | 9.97E-66 | 203 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011676.01.T01 | Q9S7A3 | 64.211 | 95 | 31 | 1 | 11 | 102 | 2 | 96 | 4.15E-36 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011676.01.T01 | G7IYH1 | 99.020 | 102 | 1 | 0 | 1 | 102 | 2 | 103 | 4.76e-66 | 203 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380011676.01 | MsG0380016910.01 | 0.811001 | 8.708961e-51 | 5.329770e-48 |
| MsG0380011676.01 | MsG0480022707.01 | 0.825868 | 3.707140e-54 | 3.420874e-51 |
| MsG0380011676.01 | MsG0780040858.01 | 0.806238 | 9.063647e-50 | 4.894691e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011676.01.T01 | MTR_3g008540 | 99.020 | 102 | 1 | 0 | 1 | 102 | 2 | 103 | 1.21e-69 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011676.01.T01 | AT4G10100 | 64.211 | 95 | 31 | 1 | 11 | 102 | 2 | 96 | 4.23e-37 | 120 |
| MsG0380011676.01.T01 | AT4G10100 | 64.211 | 95 | 31 | 1 | 11 | 102 | 2 | 96 | 4.23e-37 | 120 |
| MsG0380011676.01.T01 | AT4G10100 | 64.211 | 95 | 31 | 1 | 11 | 102 | 2 | 96 | 4.23e-37 | 120 |
Find 28 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAAGGAAATGTTAGTATT+TGG | 0.159129 | 3:+3362665 | MsG0380011676.01.T01:CDS |
| CCAAGTTTGGAAGAAATAAA+AGG | 0.205219 | 3:+3362850 | MsG0380011676.01.T01:CDS |
| TTAATTCAACCACCACTTAT+TGG | 0.233813 | 3:-3362955 | None:intergenic |
| TCTTGTTCAATTTCCAAGTT+TGG | 0.290022 | 3:+3362837 | MsG0380011676.01.T01:CDS |
| TTTCTCTCTTGGTTTGAGTT+TGG | 0.311075 | 3:-3362696 | None:intergenic |
| GCACGAGCGCGGGATCTTAC+CGG | 0.343720 | 3:+3362751 | MsG0380011676.01.T01:CDS |
| ACCACTTATTGGAGGTATAA+TGG | 0.347886 | 3:-3362944 | None:intergenic |
| GGAAGGAAATGTTAGTATTT+GGG | 0.378443 | 3:+3362666 | MsG0380011676.01.T01:CDS |
| AACCAAGAGAGAAAGTTCTT+TGG | 0.387525 | 3:+3362705 | MsG0380011676.01.T01:CDS |
| GTGCCATTGGAGGTGTCATC+TGG | 0.398317 | 3:+3362784 | MsG0380011676.01.T01:CDS |
| TACCAAAGAACTTTCTCTCT+TGG | 0.404179 | 3:-3362707 | None:intergenic |
| CGGCTTGAGCGAGGTGCCAT+TGG | 0.416694 | 3:+3362771 | MsG0380011676.01.T01:CDS |
| CTCATCCTTGTCTTTAACAA+TGG | 0.440598 | 3:-3362917 | None:intergenic |
| CAAGTTTGGAAGAAATAAAA+GGG | 0.452506 | 3:+3362851 | MsG0380011676.01.T01:CDS |
| CTTGAGCGAGGTGCCATTGG+AGG | 0.470713 | 3:+3362774 | MsG0380011676.01.T01:CDS |
| CATGGTTCTTGCTCTTAATG+AGG | 0.506386 | 3:+3362876 | MsG0380011676.01.T01:CDS |
| GGAAATGTTAGTATTTGGGA+TGG | 0.520044 | 3:+3362670 | MsG0380011676.01.T01:CDS |
| TGTTAAAGACAAGGATGAGT+TGG | 0.525125 | 3:+3362921 | MsG0380011676.01.T01:CDS |
| ATTCAACCACCACTTATTGG+AGG | 0.552937 | 3:-3362952 | None:intergenic |
| GCCATTATACCTCCAATAAG+TGG | 0.555058 | 3:+3362943 | MsG0380011676.01.T01:CDS |
| GGAAGAAATAAAAGGGTGCA+TGG | 0.556522 | 3:+3362858 | MsG0380011676.01.T01:CDS |
| CCTAAGATATTTATGATGGA+AGG | 0.558494 | 3:+3362649 | None:intergenic |
| CAATGGCACCTCGCTCAAGC+CGG | 0.577981 | 3:-3362770 | None:intergenic |
| CTTCCAGATGACACCTCCAA+TGG | 0.605197 | 3:-3362787 | None:intergenic |
| GGATCTTACCGGCTTGAGCG+AGG | 0.618028 | 3:+3362762 | MsG0380011676.01.T01:CDS |
| TTCAACCATTGTTAAAGACA+AGG | 0.621442 | 3:+3362912 | MsG0380011676.01.T01:CDS |
| TCTTAATGAGGAGTATACAA+TGG | 0.669332 | 3:+3362888 | MsG0380011676.01.T01:CDS |
| ATTATACCTCCAATAAGTGG+TGG | 0.676111 | 3:+3362946 | MsG0380011676.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAAGTTTGGAAGAAATAAAA+GGG | + | Chr3:3362851-3362870 | MsG0380011676.01.T01:CDS | 25.0% |
| CCAAGTTTGGAAGAAATAAA+AGG | + | Chr3:3362850-3362869 | MsG0380011676.01.T01:CDS | 30.0% | |
| GGAAGGAAATGTTAGTATTT+GGG | + | Chr3:3362666-3362685 | MsG0380011676.01.T01:CDS | 30.0% | |
| TCTTGTTCAATTTCCAAGTT+TGG | + | Chr3:3362837-3362856 | MsG0380011676.01.T01:CDS | 30.0% | |
| TGGAAGGAAATGTTAGTATT+TGG | + | Chr3:3362665-3362684 | MsG0380011676.01.T01:CDS | 30.0% | |
| TTCAACCATTGTTAAAGACA+AGG | + | Chr3:3362912-3362931 | MsG0380011676.01.T01:CDS | 30.0% | |
| ! | TCTTAATGAGGAGTATACAA+TGG | + | Chr3:3362888-3362907 | MsG0380011676.01.T01:CDS | 30.0% |
| !! | CCTTTTATTTCTTCCAAACT+TGG | - | Chr3:3362853-3362872 | None:intergenic | 30.0% |
| AACCAAGAGAGAAAGTTCTT+TGG | + | Chr3:3362705-3362724 | MsG0380011676.01.T01:CDS | 35.0% | |
| ATTATACCTCCAATAAGTGG+TGG | + | Chr3:3362946-3362965 | MsG0380011676.01.T01:CDS | 35.0% | |
| CTCATCCTTGTCTTTAACAA+TGG | - | Chr3:3362920-3362939 | None:intergenic | 35.0% | |
| GGAAATGTTAGTATTTGGGA+TGG | + | Chr3:3362670-3362689 | MsG0380011676.01.T01:CDS | 35.0% | |
| TACCAAAGAACTTTCTCTCT+TGG | - | Chr3:3362710-3362729 | None:intergenic | 35.0% | |
| TGTTAAAGACAAGGATGAGT+TGG | + | Chr3:3362921-3362940 | MsG0380011676.01.T01:CDS | 35.0% | |
| ! | ACCACTTATTGGAGGTATAA+TGG | - | Chr3:3362947-3362966 | None:intergenic | 35.0% |
| ! | TTTCTCTCTTGGTTTGAGTT+TGG | - | Chr3:3362699-3362718 | None:intergenic | 35.0% |
| CATGGTTCTTGCTCTTAATG+AGG | + | Chr3:3362876-3362895 | MsG0380011676.01.T01:CDS | 40.0% | |
| GCCATTATACCTCCAATAAG+TGG | + | Chr3:3362943-3362962 | MsG0380011676.01.T01:CDS | 40.0% | |
| ! | GGAAGAAATAAAAGGGTGCA+TGG | + | Chr3:3362858-3362877 | MsG0380011676.01.T01:CDS | 40.0% |
| CTTCCAGATGACACCTCCAA+TGG | - | Chr3:3362790-3362809 | None:intergenic | 50.0% | |
| !!! | TGCTGTTTTTTGCACGAGCG+CGG | + | Chr3:3362740-3362759 | MsG0380011676.01.T01:CDS | 50.0% |
| !! | GTGCCATTGGAGGTGTCATC+TGG | + | Chr3:3362784-3362803 | MsG0380011676.01.T01:CDS | 55.0% |
| !!! | GCTGTTTTTTGCACGAGCGC+GGG | + | Chr3:3362741-3362760 | MsG0380011676.01.T01:CDS | 55.0% |
| CAATGGCACCTCGCTCAAGC+CGG | - | Chr3:3362773-3362792 | None:intergenic | 60.0% | |
| !! | CTTGAGCGAGGTGCCATTGG+AGG | + | Chr3:3362774-3362793 | MsG0380011676.01.T01:CDS | 60.0% |
| !! | GGATCTTACCGGCTTGAGCG+AGG | + | Chr3:3362762-3362781 | MsG0380011676.01.T01:CDS | 60.0% |
| GCACGAGCGCGGGATCTTAC+CGG | + | Chr3:3362751-3362770 | MsG0380011676.01.T01:CDS | 65.0% | |
| ! | CGGCTTGAGCGAGGTGCCAT+TGG | + | Chr3:3362771-3362790 | MsG0380011676.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 3362664 | 3362972 | 3362664 | ID=MsG0380011676.01;Name=MsG0380011676.01 |
| Chr3 | mRNA | 3362664 | 3362972 | 3362664 | ID=MsG0380011676.01.T01;Parent=MsG0380011676.01;Name=MsG0380011676.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|102 |
| Chr3 | exon | 3362664 | 3362972 | 3362664 | ID=MsG0380011676.01.T01:exon:999;Parent=MsG0380011676.01.T01 |
| Chr3 | CDS | 3362664 | 3362972 | 3362664 | ID=MsG0380011676.01.T01:cds;Parent=MsG0380011676.01.T01 |
| Gene Sequence |
| Protein sequence |
