AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040858.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040858.01.T01 MTR_7g099950 81.250 288 17 1 1 288 85 335 1.80e-161 452
MsG0780040858.01.T01 MTR_1g068825 40.068 292 121 6 1 287 1 243 4.24e-39 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040858.01.T01 AT3G53670 33.083 133 83 1 98 230 51 177 6.60e-13 66.6
MsG0780040858.01.T01 AT3G53670 33.083 133 83 1 98 230 51 177 6.60e-13 66.6
MsG0780040858.01.T01 AT2G37480 47.126 87 41 2 150 231 85 171 3.05e-12 64.7
MsG0780040858.01.T01 AT2G37480 47.674 86 40 2 151 231 87 172 3.82e-12 64.3

Find 54 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTTATGCACATGAAAT+AGG 0.221808 7:+83504614 None:intergenic
TATGTATTATAAAGGAAATT+TGG 0.264568 7:-83504398 MsG0780040858.01.T01:three_prime_UTR
TCATGGCTTATGTATTATAA+AGG 0.273130 7:-83504406 MsG0780040858.01.T01:three_prime_UTR
TGCTTCCGCAGTTTGAATCT+TGG 0.342574 7:+83504716 None:intergenic
GGCAACGTCGCATGAGGAAT+TGG 0.368193 7:+83505508 None:intergenic
GTAAAATATCTTGAATGCTT+TGG 0.371014 7:-83504637 MsG0780040858.01.T01:three_prime_UTR
CAACAAATCAATACCACATA+TGG 0.392250 7:+83505286 None:intergenic
ATAGGAAATTGACGGTGAAA+TGG 0.412052 7:-83504996 MsG0780040858.01.T01:CDS
TTAATGATGCTTATGTGTAT+TGG 0.412073 7:-83505360 MsG0780040858.01.T01:CDS
TTTATCACGGCTACCTTTCA+AGG 0.425025 7:+83505032 None:intergenic
ACAAGAAACAGCCGAAATCC+AGG 0.447680 7:-83504921 MsG0780040858.01.T01:CDS
TAGGAAATTGACGGTGAAAT+GGG 0.449608 7:-83504995 MsG0780040858.01.T01:CDS
TGGGATTCTCAAGTTATTGA+AGG 0.461070 7:-83504772 MsG0780040858.01.T01:CDS
TCCTCGTCAGACGAGCTTGA+AGG 0.485645 7:+83505487 None:intergenic
GTATATATAGTGAAAAGCTT+GGG 0.495142 7:-83504570 MsG0780040858.01.T01:three_prime_UTR
AAGCAGTCTCGCAACGTTGG+TGG 0.501681 7:-83504820 MsG0780040858.01.T01:CDS
GTGAAAAGCTTGGGATTGTA+TGG 0.514719 7:-83504561 MsG0780040858.01.T01:three_prime_UTR
TGTATATATAGTGAAAAGCT+TGG 0.519873 7:-83504571 MsG0780040858.01.T01:three_prime_UTR
CAGCTTCAAACTGCCTCTGA+AGG 0.523218 7:+83504522 None:intergenic
AAGGCAGATTTGAGCTCCGA+AGG 0.526871 7:+83505051 None:intergenic
AGCTGGCGTTATTCCATATG+TGG 0.531457 7:-83505299 MsG0780040858.01.T01:intron
AGCAGTCTCGCAACGTTGGT+GGG 0.540201 7:-83504819 MsG0780040858.01.T01:CDS
TGCGACACTAGTGTAGGTAT+AGG 0.544332 7:+83504955 None:intergenic
TTCACCGTCAATTTCCTATG+TGG 0.544822 7:+83505000 None:intergenic
AAATGTAAAGAAAGGTAACA+AGG 0.560400 7:-83504878 MsG0780040858.01.T01:CDS
AATGTAAAGAAAGGTAACAA+GGG 0.568123 7:-83504877 MsG0780040858.01.T01:CDS
GAGCTCAAATCTGCCTTGAA+AGG 0.568787 7:-83505045 MsG0780040858.01.T01:CDS
GAAATCTGAACATTTGTCTG+AGG 0.568873 7:-83505632 MsG0780040858.01.T01:CDS
AAATCTGAACATTTGTCTGA+GGG 0.573710 7:-83505631 MsG0780040858.01.T01:CDS
GCCTTCAAGCTCGTCTGACG+AGG 0.576208 7:-83505488 MsG0780040858.01.T01:CDS
TGAACCACATAGGAAATTGA+CGG 0.581672 7:-83505004 MsG0780040858.01.T01:CDS
CTGATCTGTGCAGTAAGTCG+TGG 0.587620 7:-83504792 MsG0780040858.01.T01:CDS
TGCGATGTGCAATGTAGCCA+AGG 0.589787 7:-83505605 MsG0780040858.01.T01:CDS
ATCTGAACATTTGTCTGAGG+GGG 0.595296 7:-83505629 MsG0780040858.01.T01:CDS
CTTGAAGGCAACGTCGCATG+AGG 0.601933 7:+83505502 None:intergenic
AGAAGTGCATTACTCAGTTG+CGG 0.604827 7:-83504671 MsG0780040858.01.T01:CDS
TGATCTGTGCAGTAAGTCGT+GGG 0.608135 7:-83504791 MsG0780040858.01.T01:CDS
AGCTTCAAACTGCCTCTGAA+GGG 0.608595 7:+83504523 None:intergenic
AAGAAGCAGTCTCGCAACGT+TGG 0.609694 7:-83504823 MsG0780040858.01.T01:CDS
TAGCCAAGGAAAACTCAAGT+GGG 0.611731 7:-83505591 MsG0780040858.01.T01:CDS
ATCAAGAGAGTCAAATTCAG+AGG 0.614512 7:+83504747 None:intergenic
ATGTAAAGAAAGGTAACAAG+GGG 0.626414 7:-83504876 MsG0780040858.01.T01:CDS
TGATGAGCAGAATGCTCATG+AGG 0.641178 7:+83505439 None:intergenic
AAGGGGAATTCAACCCGAGG+GGG 0.642568 7:-83504859 MsG0780040858.01.T01:CDS
GAAAGAATCAAAACCCAACA+TGG 0.643797 7:-83505557 MsG0780040858.01.T01:CDS
CGTCGCATGAGGAATTGGAA+AGG 0.653621 7:+83505513 None:intergenic
ACAGTGTGCGACACTAGTGT+AGG 0.663479 7:+83504949 None:intergenic
TGTAGCCAAGATTCAAACTG+CGG 0.664125 7:-83504721 MsG0780040858.01.T01:CDS
GTAGCCAAGGAAAACTCAAG+TGG 0.667603 7:-83505592 MsG0780040858.01.T01:CDS
AATCTGAACATTTGTCTGAG+GGG 0.684222 7:-83505630 MsG0780040858.01.T01:CDS
AACAAGGGGAATTCAACCCG+AGG 0.692269 7:-83504862 MsG0780040858.01.T01:CDS
ACAAGGGGAATTCAACCCGA+GGG 0.697575 7:-83504861 MsG0780040858.01.T01:CDS
AGCCAAGGAAAACTCAAGTG+GGG 0.705918 7:-83505590 MsG0780040858.01.T01:CDS
CAAGGGGAATTCAACCCGAG+GGG 0.745728 7:-83504860 MsG0780040858.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGCTAAATTAAAAAAAAAA+CGG + Chr7:83504987-83505006 None:intergenic 15.0%
!! TATGTATTATAAAGGAAATT+TGG - Chr7:83505711-83505730 MsG0780040858.01.T01:CDS 15.0%
!! AGTTTAACACTATATTGAAT+AGG + Chr7:83504893-83504912 None:intergenic 20.0%
!! ATTGAATAGGTAAATTCAAT+AGG + Chr7:83504880-83504899 None:intergenic 20.0%
!! GAAAAGAAAAATGTAAAGAA+AGG - Chr7:83505223-83505242 MsG0780040858.01.T01:intron 20.0%
!! GATGTAAAATACATAGAAAA+AGG + Chr7:83505031-83505050 None:intergenic 20.0%
!! TCAATATAGTGTTAAACTAA+TGG - Chr7:83504895-83504914 MsG0780040858.01.T01:CDS 20.0%
!! TTTATTTATGCACATGAAAT+AGG + Chr7:83505498-83505517 None:intergenic 20.0%
! AAATGTAAAGAAAGGTAACA+AGG - Chr7:83505231-83505250 MsG0780040858.01.T01:intron 25.0%
! AATGTAAAGAAAGGTAACAA+GGG - Chr7:83505232-83505251 MsG0780040858.01.T01:intron 25.0%
! GTAAAATATCTTGAATGCTT+TGG - Chr7:83505472-83505491 MsG0780040858.01.T01:CDS 25.0%
! GTATATATAGTGAAAAGCTT+GGG - Chr7:83505539-83505558 MsG0780040858.01.T01:CDS 25.0%
! TCATGGCTTATGTATTATAA+AGG - Chr7:83505703-83505722 MsG0780040858.01.T01:CDS 25.0%
! TGTATATATAGTGAAAAGCT+TGG - Chr7:83505538-83505557 MsG0780040858.01.T01:CDS 25.0%
! TTAATGATGCTTATGTGTAT+TGG - Chr7:83504749-83504768 MsG0780040858.01.T01:CDS 25.0%
! TTGTAACATGAAAAACTTAC+AGG + Chr7:83504721-83504740 None:intergenic 25.0%
!! AGTTTTTCATGTTACAAAGT+CGG - Chr7:83504724-83504743 MsG0780040858.01.T01:CDS 25.0%
!! TTCTATGTATTTTACATCAC+AGG - Chr7:83505033-83505052 MsG0780040858.01.T01:CDS 25.0%
!!! AATTTCTGTTTTGAAAAAGC+TGG - Chr7:83504793-83504812 MsG0780040858.01.T01:CDS 25.0%
!!! ACACATTTTTGTTTGTAATG+TGG - Chr7:83505639-83505658 MsG0780040858.01.T01:CDS 25.0%
!!! TTGTTGTTTTTATGCTTTCA+TGG - Chr7:83505686-83505705 MsG0780040858.01.T01:CDS 25.0%
AAATCTGAACATTTGTCTGA+GGG - Chr7:83504478-83504497 MsG0780040858.01.T01:three_prime_UTR 30.0%
ATGTAAAGAAAGGTAACAAG+GGG - Chr7:83505233-83505252 MsG0780040858.01.T01:intron 30.0%
CAACAAATCAATACCACATA+TGG + Chr7:83504826-83504845 None:intergenic 30.0%
TGTCCGAATAACAAAAAGAA+CGG + Chr7:83504932-83504951 None:intergenic 30.0%
! CTTTTCATTCTTCTTTTTCC+TGG + Chr7:83505209-83505228 None:intergenic 30.0%
! TTCTTCTTTTTCCTGGATTT+CGG + Chr7:83505202-83505221 None:intergenic 30.0%
!! CATATGTGGTATTGATTTGT+TGG - Chr7:83504824-83504843 MsG0780040858.01.T01:CDS 30.0%
!! TTTTTCAAACACACCATGTT+GGG + Chr7:83504568-83504587 None:intergenic 30.0%
!!! ACACCGTTCTTTTTGTTATT+CGG - Chr7:83504926-83504945 MsG0780040858.01.T01:CDS 30.0%
!!! TTTTTTCAAACACACCATGT+TGG + Chr7:83504569-83504588 None:intergenic 30.0%
AAAGGTAGCCGTGATAAAAA+CGG - Chr7:83505082-83505101 MsG0780040858.01.T01:intron 35.0%
AATCTGAACATTTGTCTGAG+GGG - Chr7:83504479-83504498 MsG0780040858.01.T01:three_prime_UTR 35.0%
AGCTGTTGAAAAAAATGCAC+AGG + Chr7:83505007-83505026 None:intergenic 35.0%
ATAAAAACGGTGAACCACAT+AGG - Chr7:83505095-83505114 MsG0780040858.01.T01:intron 35.0%
ATAGGAAATTGACGGTGAAA+TGG - Chr7:83505113-83505132 MsG0780040858.01.T01:intron 35.0%
ATCAAGAGAGTCAAATTCAG+AGG + Chr7:83505365-83505384 None:intergenic 35.0%
CAATAGGAATGCTTAACAGA+TGG + Chr7:83504864-83504883 None:intergenic 35.0%
GAAAGAATCAAAACCCAACA+TGG - Chr7:83504552-83504571 MsG0780040858.01.T01:three_prime_UTR 35.0%
GAAATCTGAACATTTGTCTG+AGG - Chr7:83504477-83504496 MsG0780040858.01.T01:three_prime_UTR 35.0%
TAGGAAATTGACGGTGAAAT+GGG - Chr7:83505114-83505133 MsG0780040858.01.T01:intron 35.0%
TGAACCACATAGGAAATTGA+CGG - Chr7:83505105-83505124 MsG0780040858.01.T01:intron 35.0%
TGGGATTCTCAAGTTATTGA+AGG - Chr7:83505337-83505356 MsG0780040858.01.T01:CDS 35.0%
! GAAATTTTTGTACCCTTCAG+AGG - Chr7:83505574-83505593 MsG0780040858.01.T01:CDS 35.0%
ATCTGAACATTTGTCTGAGG+GGG - Chr7:83504480-83504499 MsG0780040858.01.T01:three_prime_UTR 40.0%
GTGAAAAGCTTGGGATTGTA+TGG - Chr7:83505548-83505567 MsG0780040858.01.T01:CDS 40.0%
TAGCCAAGGAAAACTCAAGT+GGG - Chr7:83504518-83504537 MsG0780040858.01.T01:three_prime_UTR 40.0%
TGTAGCCAAGATTCAAACTG+CGG - Chr7:83505388-83505407 MsG0780040858.01.T01:CDS 40.0%
TTCACCGTCAATTTCCTATG+TGG + Chr7:83505112-83505131 None:intergenic 40.0%
TTTATCACGGCTACCTTTCA+AGG + Chr7:83505080-83505099 None:intergenic 40.0%
! AGAAGTGCATTACTCAGTTG+CGG - Chr7:83505438-83505457 MsG0780040858.01.T01:CDS 40.0%
! TTTTACATCACAGGCTCCTT+CGG - Chr7:83505042-83505061 MsG0780040858.01.T01:CDS 40.0%
!! GTGGTTCACCGTTTTTATCA+CGG + Chr7:83505093-83505112 None:intergenic 40.0%
ACAAGAAACAGCCGAAATCC+AGG - Chr7:83505188-83505207 MsG0780040858.01.T01:intron 45.0%
AGCCAAGGAAAACTCAAGTG+GGG - Chr7:83504519-83504538 MsG0780040858.01.T01:three_prime_UTR 45.0%
AGCTGGCGTTATTCCATATG+TGG - Chr7:83504810-83504829 MsG0780040858.01.T01:CDS 45.0%
AGCTTCAAACTGCCTCTGAA+GGG + Chr7:83505589-83505608 None:intergenic 45.0%
GAGCTCAAATCTGCCTTGAA+AGG - Chr7:83505064-83505083 MsG0780040858.01.T01:intron 45.0%
GTAGCCAAGGAAAACTCAAG+TGG - Chr7:83504517-83504536 MsG0780040858.01.T01:three_prime_UTR 45.0%
TGATCTGTGCAGTAAGTCGT+GGG - Chr7:83505318-83505337 MsG0780040858.01.T01:CDS 45.0%
TGATGAGCAGAATGCTCATG+AGG + Chr7:83504673-83504692 None:intergenic 45.0%
TGCGACACTAGTGTAGGTAT+AGG + Chr7:83505157-83505176 None:intergenic 45.0%
TGCTTCCGCAGTTTGAATCT+TGG + Chr7:83505396-83505415 None:intergenic 45.0%
! AACCCCACTTGAGTTTTCCT+TGG + Chr7:83504524-83504543 None:intergenic 45.0%
AACAAGGGGAATTCAACCCG+AGG - Chr7:83505247-83505266 MsG0780040858.01.T01:intron 50.0%
AAGAAGCAGTCTCGCAACGT+TGG - Chr7:83505286-83505305 MsG0780040858.01.T01:intron 50.0%
AAGGCAGATTTGAGCTCCGA+AGG + Chr7:83505061-83505080 None:intergenic 50.0%
ACAAGGGGAATTCAACCCGA+GGG - Chr7:83505248-83505267 MsG0780040858.01.T01:intron 50.0%
ACAGTGTGCGACACTAGTGT+AGG + Chr7:83505163-83505182 None:intergenic 50.0%
CAGCTTCAAACTGCCTCTGA+AGG + Chr7:83505590-83505609 None:intergenic 50.0%
CGTCGCATGAGGAATTGGAA+AGG + Chr7:83504599-83504618 None:intergenic 50.0%
CTGATCTGTGCAGTAAGTCG+TGG - Chr7:83505317-83505336 MsG0780040858.01.T01:CDS 50.0%
TATCTTTGCCATTGCCCCCT+CGG + Chr7:83505267-83505286 None:intergenic 50.0%
TGCGATGTGCAATGTAGCCA+AGG - Chr7:83504504-83504523 MsG0780040858.01.T01:three_prime_UTR 50.0%
AAGCAGTCTCGCAACGTTGG+TGG - Chr7:83505289-83505308 MsG0780040858.01.T01:intron 55.0%
AAGGGGAATTCAACCCGAGG+GGG - Chr7:83505250-83505269 MsG0780040858.01.T01:intron 55.0%
AATTCAACCCGAGGGGGCAA+TGG - Chr7:83505256-83505275 MsG0780040858.01.T01:intron 55.0%
AGCAGTCTCGCAACGTTGGT+GGG - Chr7:83505290-83505309 MsG0780040858.01.T01:intron 55.0%
ATCTTTGCCATTGCCCCCTC+GGG + Chr7:83505266-83505285 None:intergenic 55.0%
CAAGGGGAATTCAACCCGAG+GGG - Chr7:83505249-83505268 MsG0780040858.01.T01:intron 55.0%
CTTGAAGGCAACGTCGCATG+AGG + Chr7:83504610-83504629 None:intergenic 55.0%
GGCAACGTCGCATGAGGAAT+TGG + Chr7:83504604-83504623 None:intergenic 55.0%
TCCTCGTCAGACGAGCTTGA+AGG + Chr7:83504625-83504644 None:intergenic 55.0%
GCCTTCAAGCTCGTCTGACG+AGG - Chr7:83504621-83504640 MsG0780040858.01.T01:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 83504382 83505749 83504382 ID=MsG0780040858.01;Name=MsG0780040858.01
Chr7 mRNA 83504382 83505749 83504382 ID=MsG0780040858.01.T01;Parent=MsG0780040858.01;Name=MsG0780040858.01.T01;_AED=0.34;_eAED=0.40;_QI=0|0|0.5|1|1|1|2|278|288
Chr7 exon 83504382 83505076 83504382 ID=MsG0780040858.01.T01:exon:19789;Parent=MsG0780040858.01.T01
Chr7 exon 83505300 83505749 83505300 ID=MsG0780040858.01.T01:exon:19788;Parent=MsG0780040858.01.T01
Chr7 CDS 83505300 83505749 83505300 ID=MsG0780040858.01.T01:cds;Parent=MsG0780040858.01.T01
Chr7 CDS 83504660 83505076 83504660 ID=MsG0780040858.01.T01:cds;Parent=MsG0780040858.01.T01
Chr7 three_prime_UTR 83504382 83504659 83504382 ID=MsG0780040858.01.T01:three_prime_utr;Parent=MsG0780040858.01.T01
Gene Sequence

>MsG0780040858.01.T01

ATGGAAGTGTGCTCTTCTGTTAATGACATGCACGAGAACTGCATAAGTAATCTCGAAAGCACTTTCAGCAAGTCATTGCATATACAAGATACTGAGAAATCTGAACATTTGTCTGAGGGGGATGCGATGTGCAATGTAGCCAAGGAAAACTCAAGTGGGGTTAAAAAACAGAAAGAATCAAAACCCAACATGGTGTGTTTGAAAAAATCTGCAACCTTTCCAATTCCTCATGCGACGTTGCCTTCAAGCTCGTCTGACGAGGACGCTGATTCATCAGTTATAGAATCGCCTCATGAGCATTCTGCTCATCAAGCTTACTCTCGTTCGATATCTCTGCCTGTAAGTTTTTCATGTTACAAAGTCGGTGTTAATGATGCTTATGTGTATTGGTTTCAAGCAATTATGCTTCTTAATTTCTGTTTTGAAAAAGCTGGCGTTATTCCATATGTGGCTCCTTCGGAGCTCAAATCTGCCTTGAAAGGTAGCCGTGATAAAAACGGTGAACCACATAGGAAATTGACGGTGAAATGGGCTGCTGATGTGTATGATCCTATACCTACACTAGTGTCGCACACTGTCAAAAACAAGAAACAGCCGAAATCCAGGAAAAAGAAGAATGAAAAGAAAAATGTAAAGAAAGGTAACAAGGGGAATTCAACCCGAGGGGGCAATGGCAAAGATAAGAAGCAGTCTCGCAACGTTGGTGGGCAATCTGATCTGTGCAGTAAGTCGTGGGATTCTCAAGTTATTGAAGGTTCCTCTGAATTTGACTCTCTTGATGTTTGTAGCCAAGATTCAAACTGCGGAAGCAGTTTCTTGAAAAAATCTGTTACAGAAGTGCATTACTCAGTTGCGGAAGCACAATGA

Protein sequence

>MsG0780040858.01.T01

MEVCSSVNDMHENCISNLESTFSKSLHIQDTEKSEHLSEGDAMCNVAKENSSGVKKQKESKPNMVCLKKSATFPIPHATLPSSSSDEDADSSVIESPHEHSAHQAYSRSISLPVSFSCYKVGVNDAYVYWFQAIMLLNFCFEKAGVIPYVAPSELKSALKGSRDKNGEPHRKLTVKWAADVYDPIPTLVSHTVKNKKQPKSRKKKNEKKNVKKGNKGNSTRGGNGKDKKQSRNVGGQSDLCSKSWDSQVIEGSSEFDSLDVCSQDSNCGSSFLKKSVTEVHYSVAEAQ*