AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380011772.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380011772.01.T01 MTR_3g030250 74.054 185 46 1 19 201 196 380 1.10e-93 278
MsG0380011772.01.T01 MTR_3g006500 73.797 187 45 2 19 201 184 370 2.90e-92 274
MsG0380011772.01.T01 MTR_3g030240 71.351 185 50 2 17 201 197 378 4.74e-90 269
MsG0380011772.01.T01 MTR_3g023930 68.649 185 57 1 17 201 185 368 2.84e-83 251
MsG0380011772.01.T01 MTR_3g010520 67.742 186 58 2 17 201 176 360 5.98e-81 245
MsG0380011772.01.T01 MTR_3g010590 63.351 191 61 3 19 201 197 386 5.58e-74 228
MsG0380011772.01.T01 MTR_3g010870 60.302 199 72 3 9 201 188 385 4.20e-73 226
MsG0380011772.01.T01 MTR_3g010920 62.105 190 64 4 17 201 196 382 8.27e-72 223
MsG0380011772.01.T01 MTR_0044s0300 54.359 195 79 3 17 201 196 390 2.19e-69 216
MsG0380011772.01.T01 MTR_3g010970 59.677 186 68 3 20 201 210 392 2.05e-68 214
MsG0380011772.01.T01 MTR_3g024150 59.067 193 71 3 17 201 194 386 2.97e-68 213
MsG0380011772.01.T01 MTR_3g010870 61.236 178 63 2 30 201 140 317 2.52e-67 209
MsG0380011772.01.T01 MTR_3g010880 60.326 184 54 4 36 201 159 341 8.39e-67 208
MsG0380011772.01.T01 MTR_3g023940 57.447 188 60 3 28 201 203 384 8.65e-65 204
MsG0380011772.01.T01 MTR_3g030330 53.623 207 79 5 9 201 169 372 4.29e-63 200
MsG0380011772.01.T01 MTR_6g065980 53.763 186 80 4 22 201 174 359 1.91e-60 192
MsG0380011772.01.T01 MTR_2g078870 51.031 194 82 4 18 201 118 308 5.04e-56 180
MsG0380011772.01.T01 MTR_3g036430 52.486 181 83 3 23 201 164 343 1.42e-55 180
MsG0380011772.01.T01 MTR_1g113900 50.515 194 87 4 15 201 55 246 7.16e-55 175
MsG0380011772.01.T01 MTR_3g010620 52.195 205 74 6 17 201 193 393 8.87e-55 179
MsG0380011772.01.T01 MTR_3g024110 51.892 185 84 3 22 201 264 448 3.22e-54 179
MsG0380011772.01.T01 MTR_1004s0010 52.174 184 82 3 22 201 179 360 7.00e-54 176
MsG0380011772.01.T01 MTR_2g078850 58.442 154 59 3 36 184 214 367 8.88e-54 176
MsG0380011772.01.T01 MTR_1g115160 47.917 192 87 4 20 201 179 367 9.60e-54 176
MsG0380011772.01.T01 MTR_0760s0010 52.174 184 82 3 22 201 209 390 1.11e-53 176
MsG0380011772.01.T01 MTR_3g030320 52.128 188 78 5 24 201 114 299 1.62e-53 173
MsG0380011772.01.T01 MTR_2g021190 51.872 187 77 4 22 201 193 373 1.95e-53 175
MsG0380011772.01.T01 MTR_3g083660 50.556 180 86 3 22 198 99 278 4.07e-52 169
MsG0380011772.01.T01 MTR_3g006570 49.733 187 87 5 22 201 191 377 1.73e-50 167
MsG0380011772.01.T01 MTR_5g046280 48.404 188 89 4 20 201 69 254 5.68e-49 160
MsG0380011772.01.T01 MTR_1g112930 49.490 196 87 6 13 200 184 375 5.79e-49 164
MsG0380011772.01.T01 MTR_5g046300 48.936 188 88 4 20 201 166 351 7.07e-49 162
MsG0380011772.01.T01 MTR_5g046290 48.936 188 88 4 20 201 166 351 7.07e-49 162
MsG0380011772.01.T01 MTR_3g030310 46.829 205 86 6 15 201 192 391 9.98e-49 163
MsG0380011772.01.T01 MTR_3g030350 50.259 193 83 6 22 201 199 391 1.70e-48 162
MsG0380011772.01.T01 MTR_2g029990 48.421 190 82 4 22 201 220 403 4.88e-48 162
MsG0380011772.01.T01 MTR_2g078900 53.548 155 64 3 18 164 193 347 1.02e-47 160
MsG0380011772.01.T01 MTR_3g006530 48.421 190 93 3 15 201 210 397 1.37e-47 160
MsG0380011772.01.T01 MTR_1g114650 47.090 189 85 5 25 201 46 231 7.38e-47 154
MsG0380011772.01.T01 MTR_3g036335 46.032 189 95 4 18 201 209 395 2.75e-46 157
MsG0380011772.01.T01 MTR_3g010430 46.117 206 93 6 4 201 198 393 4.57e-46 157
MsG0380011772.01.T01 MTR_3g006640 43.939 198 94 6 19 200 96 292 6.12e-43 145
MsG0380011772.01.T01 MTR_3g083640 45.161 186 92 3 22 201 222 403 7.83e-43 148
MsG0380011772.01.T01 MTR_3g024130 45.550 191 82 8 22 201 136 315 1.01e-41 143
MsG0380011772.01.T01 MTR_5g011200 46.500 200 84 8 18 201 183 375 5.37e-41 142
MsG0380011772.01.T01 MTR_3g036290 44.103 195 87 8 17 201 175 357 2.48e-40 140
MsG0380011772.01.T01 MTR_1g115185 46.597 191 87 7 24 201 212 400 3.20e-39 139
MsG0380011772.01.T01 MTR_3g013770 43.750 192 85 7 22 201 208 388 7.31e-39 137
MsG0380011772.01.T01 MTR_1g055375 43.062 209 98 6 2 201 171 367 7.92e-39 137
MsG0380011772.01.T01 MTR_3g096280 43.548 186 77 3 22 201 180 343 9.44e-39 136
MsG0380011772.01.T01 MTR_3g014540 41.791 201 93 8 13 201 190 378 1.29e-38 137
MsG0380011772.01.T01 MTR_3g006550 42.941 170 54 3 33 201 11 138 1.65e-38 129
MsG0380011772.01.T01 MTR_6g013270 43.590 195 91 6 23 201 202 393 7.40e-38 135
MsG0380011772.01.T01 MTR_4g055060 42.786 201 92 8 13 201 198 387 7.45e-38 135
MsG0380011772.01.T01 MTR_6g014720 42.784 194 94 6 23 201 184 375 1.55e-37 134
MsG0380011772.01.T01 MTR_6g007135 44.000 200 87 7 20 201 198 390 1.74e-37 134
MsG0380011772.01.T01 MTR_1g054725 42.857 189 94 6 22 201 193 376 2.77e-37 133
MsG0380011772.01.T01 MTR_3g436150 42.632 190 90 7 22 201 213 393 5.36e-37 132
MsG0380011772.01.T01 MTR_5g046260 42.703 185 66 4 20 201 151 298 5.49e-37 130
MsG0380011772.01.T01 MTR_6g021880 44.041 193 89 7 22 201 196 382 6.85e-37 132
MsG0380011772.01.T01 MTR_7g407070 42.487 193 89 7 22 201 216 399 7.54e-37 132
MsG0380011772.01.T01 MTR_2g435950 43.716 183 81 8 28 201 128 297 1.50e-36 129
MsG0380011772.01.T01 MTR_2g021155 44.318 176 81 5 22 186 203 372 1.76e-36 135
MsG0380011772.01.T01 MTR_2g021155 39.286 196 99 5 20 201 767 956 1.65e-33 126
MsG0380011772.01.T01 MTR_4g031770 40.455 220 103 7 2 201 158 369 2.39e-36 130
MsG0380011772.01.T01 MTR_7g498320 49.265 136 64 3 41 173 83 216 3.25e-36 126
MsG0380011772.01.T01 MTR_3g011020 40.625 192 92 7 22 201 190 371 8.07e-36 129
MsG0380011772.01.T01 MTR_6g007150 42.000 200 90 9 22 201 145 338 8.33e-36 128
MsG0380011772.01.T01 MTR_3g014570 41.667 192 89 8 22 201 199 379 9.95e-36 129
MsG0380011772.01.T01 MTR_2g021210 45.652 184 84 6 27 201 249 425 1.82e-35 129
MsG0380011772.01.T01 MTR_3g009240 42.424 198 94 7 14 201 196 383 2.07e-35 128
MsG0380011772.01.T01 MTR_3g010390 42.157 204 93 7 14 201 204 398 2.91e-35 128
MsG0380011772.01.T01 MTR_3g007410 43.655 197 87 9 16 200 218 402 8.20e-35 127
MsG0380011772.01.T01 MTR_3g013740 42.347 196 85 9 22 201 224 407 3.76e-34 125
MsG0380011772.01.T01 MTR_1g114700 42.857 189 93 5 24 201 204 388 7.13e-34 124
MsG0380011772.01.T01 MTR_3g009260 40.758 211 102 9 5 201 184 385 8.82e-34 124
MsG0380011772.01.T01 MTR_3g007360 43.147 197 88 9 16 200 218 402 9.74e-34 124
MsG0380011772.01.T01 MTR_3g007390 42.021 188 86 8 22 201 32 204 1.02e-33 119
MsG0380011772.01.T01 MTR_4g061340 42.308 182 87 7 25 201 208 376 1.63e-33 123
MsG0380011772.01.T01 MTR_6g048120 41.026 195 93 9 25 201 218 408 5.12e-33 122
MsG0380011772.01.T01 MTR_4g132560 39.130 207 106 8 9 201 59 259 5.73e-33 119
MsG0380011772.01.T01 MTR_3g009300 42.268 194 84 9 23 201 173 353 6.72e-33 121
MsG0380011772.01.T01 MTR_7g007300 43.407 182 90 6 30 201 268 446 1.44e-32 122
MsG0380011772.01.T01 MTR_3g006670 41.000 200 87 9 22 201 222 410 3.57e-32 120
MsG0380011772.01.T01 MTR_3g007430 41.885 191 82 9 22 201 215 387 4.94e-32 119
MsG0380011772.01.T01 MTR_3g011100 40.796 201 95 8 19 201 408 602 5.69e-32 121
MsG0380011772.01.T01 MTR_1g058190 40.909 198 93 8 22 199 176 369 7.77e-32 118
MsG0380011772.01.T01 MTR_7g013590 38.835 206 91 8 24 201 206 404 1.33e-31 118
MsG0380011772.01.T01 MTR_8g062660 42.162 185 92 7 24 201 205 381 2.40e-31 120
MsG0380011772.01.T01 MTR_6g053240 38.144 194 104 7 22 201 205 396 3.51e-31 117
MsG0380011772.01.T01 MTR_5g009060 40.625 192 88 8 22 200 220 398 6.67e-31 116
MsG0380011772.01.T01 MTR_5g007610 40.816 196 89 8 22 201 217 401 7.56e-31 116
MsG0380011772.01.T01 MTR_6g048140 37.563 197 108 7 17 201 210 403 1.05e-30 116
MsG0380011772.01.T01 MTR_6g004660 39.594 197 98 7 20 201 191 381 1.13e-30 115
MsG0380011772.01.T01 MTR_3g096250 48.889 135 51 3 69 201 228 346 2.02e-30 114
MsG0380011772.01.T01 MTR_3g011060 39.791 191 92 7 22 201 209 387 2.99e-30 114
MsG0380011772.01.T01 MTR_6g053260 36.788 193 108 6 22 201 509 700 4.13e-30 116
MsG0380011772.01.T01 MTR_2g021252 38.298 188 95 5 18 200 213 384 4.22e-30 114
MsG0380011772.01.T01 MTR_1g056270 39.037 187 96 6 22 201 47 222 5.17e-30 110
MsG0380011772.01.T01 MTR_3g024040 38.919 185 101 6 24 201 194 373 8.14e-30 113
MsG0380011772.01.T01 MTR_3g007270 38.424 203 103 10 11 201 218 410 9.65e-30 114
MsG0380011772.01.T01 MTR_6g004210 39.535 215 89 9 22 201 208 416 1.72e-29 113
MsG0380011772.01.T01 MTR_3g009310 37.037 189 98 7 23 201 214 391 5.48e-29 111
MsG0380011772.01.T01 MTR_1g056280 38.537 205 98 7 2 186 14 210 7.32e-29 107
MsG0380011772.01.T01 MTR_3g007330 38.421 190 96 6 22 201 215 393 4.03e-28 109
MsG0380011772.01.T01 MTR_1g056710 38.830 188 103 5 22 200 204 388 1.15e-27 107
MsG0380011772.01.T01 MTR_1g100763 44.242 165 81 7 18 173 134 296 1.24e-27 106
MsG0380011772.01.T01 MTR_3g007300 39.459 185 97 7 22 201 193 367 1.47e-27 107
MsG0380011772.01.T01 MTR_7g067680 40.541 185 87 8 24 199 206 376 2.58e-27 107
MsG0380011772.01.T01 MTR_1g055045 41.875 160 83 7 49 201 6 162 3.10e-27 101
MsG0380011772.01.T01 MTR_1g055065 38.220 191 108 6 20 201 99 288 6.49e-27 103
MsG0380011772.01.T01 MTR_5g026830 43.511 131 58 6 77 201 161 281 1.19e-26 103
MsG0380011772.01.T01 MTR_7g074260 43.784 185 70 6 23 201 71 227 1.71e-26 101
MsG0380011772.01.T01 MTR_1g055055 43.558 163 78 8 20 173 178 335 2.18e-26 103
MsG0380011772.01.T01 MTR_8g054320 40.116 172 80 8 41 201 142 301 2.85e-26 102
MsG0380011772.01.T01 MTR_6g027890 37.374 198 103 8 16 201 137 325 3.26e-26 103
MsG0380011772.01.T01 MTR_1g115180 46.099 141 65 4 69 200 70 208 6.06e-26 99.8
MsG0380011772.01.T01 MTR_6g027910 38.071 197 97 9 18 201 191 375 1.48e-25 102
MsG0380011772.01.T01 MTR_2g021150 35.204 196 103 9 21 201 183 369 2.74e-25 101
MsG0380011772.01.T01 MTR_0015s0100 34.225 187 94 6 19 201 96 257 1.15e-24 97.4
MsG0380011772.01.T01 MTR_1g057890 43.357 143 72 6 64 201 219 357 2.41e-24 98.6
MsG0380011772.01.T01 MTR_5g056990 35.829 187 99 7 27 201 63 240 3.10e-23 93.6
MsG0380011772.01.T01 MTR_5g057710 35.829 187 99 7 27 201 218 395 2.00e-22 93.6
MsG0380011772.01.T01 MTR_3g058470 37.725 167 88 6 47 201 210 372 8.47e-22 91.7
MsG0380011772.01.T01 MTR_1g097560 35.135 185 98 8 27 201 202 374 1.07e-21 91.7
MsG0380011772.01.T01 MTR_3g010440 39.785 186 87 10 36 201 152 332 2.57e-21 90.1
MsG0380011772.01.T01 MTR_5g057790 34.715 193 103 9 22 201 212 394 5.43e-21 89.7
MsG0380011772.01.T01 MTR_3g011660 37.879 132 66 4 77 201 4 126 5.71e-21 84.3
MsG0380011772.01.T01 MTR_5g057690 36.364 187 97 9 27 201 208 384 6.41e-20 86.7
MsG0380011772.01.T01 MTR_2g046920 32.124 193 103 9 25 201 193 373 8.87e-19 83.2
MsG0380011772.01.T01 MTR_6g007130 39.286 112 58 4 94 201 17 122 1.41e-18 78.2
MsG0380011772.01.T01 MTR_2g046800 32.821 195 105 6 22 201 210 393 2.09e-18 82.4
MsG0380011772.01.T01 MTR_1g094210 33.168 202 116 7 12 201 205 399 3.20e-18 82.0
MsG0380011772.01.T01 MTR_2g046900 29.293 198 113 7 22 201 197 385 4.16e-18 81.6
MsG0380011772.01.T01 MTR_2g046830 31.053 190 106 10 27 200 195 375 1.36e-17 80.1
MsG0380011772.01.T01 MTR_2g046860 31.792 173 89 8 22 175 81 243 1.77e-17 79.3
MsG0380011772.01.T01 MTR_6g088385 27.670 206 118 6 22 199 115 317 5.18e-17 77.8
MsG0380011772.01.T01 MTR_7g070880 32.701 211 106 12 23 200 213 420 1.25e-16 77.4
MsG0380011772.01.T01 MTR_4g133170 33.333 150 85 8 20 156 169 316 1.46e-16 77.4
MsG0380011772.01.T01 MTR_3g096270 47.872 94 42 2 15 101 155 248 1.55e-16 76.3
MsG0380011772.01.T01 MTR_2g055970 31.746 189 109 8 27 201 379 561 1.83e-16 77.4
MsG0380011772.01.T01 MTR_5g022420 30.516 213 118 10 12 201 173 378 2.78e-16 76.3
MsG0380011772.01.T01 MTR_8g101580 31.720 186 110 8 27 201 216 395 3.73e-16 76.3
MsG0380011772.01.T01 MTR_1g097490 31.522 184 104 9 27 201 221 391 5.76e-16 75.5
MsG0380011772.01.T01 MTR_3g006480 42.342 111 60 3 22 129 125 234 7.44e-16 73.9
MsG0380011772.01.T01 MTR_1g097510 40.000 115 58 4 28 132 91 204 1.25e-15 73.6
MsG0380011772.01.T01 MTR_4g055040 53.521 71 27 3 116 183 1 68 1.71e-15 68.9
MsG0380011772.01.T01 MTR_4g108820 37.273 110 64 2 27 132 181 289 1.95e-15 73.6
MsG0380011772.01.T01 MTR_2g047832 29.897 194 89 10 27 182 199 383 2.31e-15 73.9
MsG0380011772.01.T01 MTR_1g056290 40.000 160 66 5 49 201 179 315 2.48e-15 73.2
MsG0380011772.01.T01 MTR_7g056290 29.386 228 115 10 17 200 195 420 3.05e-15 73.6
MsG0380011772.01.T01 MTR_7g056390 28.761 226 119 11 17 200 191 416 6.05e-15 72.8
MsG0380011772.01.T01 MTR_1g050542 31.034 203 105 10 21 201 216 405 6.41e-15 72.8
MsG0380011772.01.T01 MTR_5g056020 34.483 145 63 6 18 131 201 344 1.04e-14 72.0
MsG0380011772.01.T01 MTR_1g054575 37.391 115 62 4 27 132 219 332 1.09e-14 72.4
MsG0380011772.01.T01 MTR_6g016285 27.477 222 113 7 22 200 201 417 1.48e-14 71.6
MsG0380011772.01.T01 MTR_5g057020 38.983 118 56 5 27 132 203 316 1.53e-14 71.2
MsG0380011772.01.T01 MTR_7g070830 30.332 211 111 11 23 200 213 420 1.75e-14 71.2
MsG0380011772.01.T01 MTR_6g007140 37.634 93 49 3 94 183 17 103 7.78e-14 66.2
MsG0380011772.01.T01 MTR_5g017515 42.553 94 49 2 15 103 98 191 8.41e-14 67.8
MsG0380011772.01.T01 MTR_1g097330 35.593 118 62 6 27 132 183 298 1.57e-13 68.6
MsG0380011772.01.T01 MTR_7g078860 34.091 132 65 4 22 131 188 319 2.31e-13 68.2
MsG0380011772.01.T01 MTR_1g094210 32.571 175 100 6 12 174 205 373 2.34e-13 68.2
MsG0380011772.01.T01 MTR_4g108810 32.143 168 89 6 51 201 78 237 2.78e-13 66.6
MsG0380011772.01.T01 MTR_0707s0020 27.962 211 111 10 22 199 240 442 2.81e-13 67.8
MsG0380011772.01.T01 MTR_7g078900 33.333 135 63 5 22 131 201 333 3.62e-13 67.4
MsG0380011772.01.T01 MTR_5g058620 32.836 134 62 5 24 131 61 192 1.17e-12 65.5
MsG0380011772.01.T01 MTR_1g058870 32.836 134 62 5 24 131 61 192 1.17e-12 65.5
MsG0380011772.01.T01 MTR_2g025420 28.500 200 120 9 23 201 196 393 1.73e-12 65.5
MsG0380011772.01.T01 MTR_3g080370 26.457 223 114 8 22 200 201 417 1.73e-12 65.5
MsG0380011772.01.T01 MTR_5g068040 24.171 211 125 7 23 199 170 379 4.64e-12 64.3
MsG0380011772.01.T01 MTR_7g056260 29.936 157 78 5 7 131 159 315 7.65e-12 63.5
MsG0380011772.01.T01 MTR_4g070550 28.934 197 114 9 24 201 192 381 1.47e-11 62.8
MsG0380011772.01.T01 MTR_8g467140 32.773 119 67 4 27 132 200 318 1.51e-11 62.8
MsG0380011772.01.T01 MTR_2g043400 29.787 141 73 5 17 131 191 331 1.62e-11 62.8
MsG0380011772.01.T01 MTR_0267s0050 29.245 212 114 9 19 201 180 384 1.63e-11 62.8
MsG0380011772.01.T01 MTR_7g061090 30.534 131 67 4 25 131 205 335 1.71e-11 62.8
MsG0380011772.01.T01 MTR_7g057270 25.792 221 120 9 19 200 198 413 1.89e-11 62.4
MsG0380011772.01.T01 MTR_2g047892 26.364 220 119 11 17 200 184 396 2.26e-11 62.4
MsG0380011772.01.T01 MTR_7g061050 28.293 205 112 8 25 200 185 383 2.34e-11 62.4
MsG0380011772.01.T01 MTR_3g013950 28.571 210 118 8 19 201 182 386 2.36e-11 62.4
MsG0380011772.01.T01 MTR_2g028140 33.065 124 67 5 24 131 198 321 2.53e-11 62.4
MsG0380011772.01.T01 MTR_3g010870 59.615 52 20 1 9 60 188 238 2.89e-11 61.6
MsG0380011772.01.T01 MTR_2g060555 30.719 153 96 6 17 161 101 251 3.96e-11 61.2
MsG0380011772.01.T01 MTR_1g015730 26.147 218 116 11 21 199 182 393 4.59e-11 61.2
MsG0380011772.01.T01 MTR_2g006360 32.479 117 65 5 24 131 178 289 5.93e-11 60.8
MsG0380011772.01.T01 MTR_0710s0010 52.542 59 28 0 143 201 10 68 6.74e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACTTTGGGGACTGATTAT+TGG 0.252847 3:+4932743 MsG0380011772.01.T01:CDS
TGATGGCATGTGGCATTATA+TGG 0.281702 3:-4932784 None:intergenic
CACAAATCACTCTTGGAATT+AGG 0.327896 3:-4932913 None:intergenic
TGAAGTTAGTGTTTATACTT+TGG 0.337424 3:+4932729 MsG0380011772.01.T01:CDS
TTGTCAGTGGCACTGTTAAT+TGG 0.342579 3:+4932818 MsG0380011772.01.T01:CDS
GAAGTTAGTGTTTATACTTT+GGG 0.360273 3:+4932730 MsG0380011772.01.T01:CDS
AAGAGTGATTTGTGGTTCAT+TGG 0.373984 3:+4932922 MsG0380011772.01.T01:CDS
CAAAACAACTATGAACAAAT+AGG 0.379427 3:+4933207 MsG0380011772.01.T01:CDS
TGATTTGTGGTTCATTGGTT+TGG 0.395727 3:+4932927 MsG0380011772.01.T01:CDS
TCAAGCTTTATATATTTATG+AGG 0.412749 3:+4933086 MsG0380011772.01.T01:CDS
GTTGTTTATGATTCCAAGAC+TGG 0.432607 3:+4933162 MsG0380011772.01.T01:CDS
TGTTCCTGACATACAAGATC+AGG 0.453740 3:+4933050 MsG0380011772.01.T01:CDS
TGACAAATGTTGCGGATTGA+TGG 0.454143 3:-4932801 None:intergenic
AATATTAAAAGTACCAGTCT+TGG 0.456386 3:-4933175 None:intergenic
TTTAGATGTTTGGATTATGA+AGG 0.458410 3:+4932990 MsG0380011772.01.T01:CDS
CTAATTCCAAGAGTGATTTG+TGG 0.466749 3:+4932914 MsG0380011772.01.T01:CDS
AATGAACCACAAATCACTCT+TGG 0.471657 3:-4932920 None:intergenic
AGACCCTGATCTTGTATGTC+AGG 0.483457 3:-4933054 None:intergenic
GTGGCATTATATGGGATGTC+TGG 0.483541 3:-4932775 None:intergenic
GTTTGGATTATGAAGGAGTA+CGG 0.489917 3:+4932997 MsG0380011772.01.T01:CDS
GTGGTTCATTGGTTTGGTGA+GGG 0.490133 3:+4932933 MsG0380011772.01.T01:CDS
CTGTTAGAATTTCATGAGAC+GGG 0.497223 3:+4933120 MsG0380011772.01.T01:CDS
GAATTTCATGAGACGGGAGG+TGG 0.498863 3:+4933126 MsG0380011772.01.T01:CDS
CAGTGGCACTGTTAATTGGT+TGG 0.504385 3:+4932822 MsG0380011772.01.T01:CDS
TGTGGTTCATTGGTTTGGTG+AGG 0.505377 3:+4932932 MsG0380011772.01.T01:CDS
CATGAAATTCTAACAGCACT+TGG 0.514325 3:-4933113 None:intergenic
AGTACGGAAATCAAGAGTCT+TGG 0.527425 3:+4933013 MsG0380011772.01.T01:CDS
TCTTTCTCTTGATTTAGTGA+AGG 0.560353 3:+4932867 MsG0380011772.01.T01:CDS
GATGGCATGTGGCATTATAT+GGG 0.560385 3:-4932783 None:intergenic
AAGTTAGTGTTTATACTTTG+GGG 0.566642 3:+4932731 MsG0380011772.01.T01:CDS
GCTGTTAGAATTTCATGAGA+CGG 0.578046 3:+4933119 MsG0380011772.01.T01:CDS
ATACAAGATCAGGGTCTCGA+AGG 0.595140 3:+4933060 MsG0380011772.01.T01:CDS
GCCCACAAAAGATGTGCCAT+CGG 0.604298 3:-4932545 None:intergenic
GATGTAATGTGCTCTTCCGA+TGG 0.618328 3:+4932529 None:intergenic
GTTCCTGACATACAAGATCA+GGG 0.641985 3:+4933051 MsG0380011772.01.T01:CDS
AGTGCCACTGACAAATGTTG+CGG 0.659613 3:-4932809 None:intergenic
TTAGAATTTCATGAGACGGG+AGG 0.667550 3:+4933123 MsG0380011772.01.T01:CDS
CAATCCGCAACATTTGTCAG+TGG 0.668666 3:+4932805 MsG0380011772.01.T01:CDS
GATCTTGTATGTCAGGAACA+CGG 0.696452 3:-4933047 None:intergenic
TGTTGCGGATTGATGGCATG+TGG 0.752299 3:-4932794 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTCATTGATAATTATA+AGG + Chr3:4932685-4932704 MsG0380011772.01.T01:intron 15.0%
!!! TTAGTTCTTATTCTTATTTT+TGG + Chr3:4932578-4932597 MsG0380011772.01.T01:intron 15.0%
!!! AACTTAATTTTCTAATGGAA+GGG - Chr3:4932609-4932628 None:intergenic 20.0%
!!! ATAAACACTAACTTCATTTT+TGG - Chr3:4932725-4932744 None:intergenic 20.0%
!!! ATGTTTTTCAATTAAGCTTT+GGG + Chr3:4932657-4932676 MsG0380011772.01.T01:intron 20.0%
!!! GCAATAACTTAATTTTCTAA+TGG - Chr3:4932614-4932633 None:intergenic 20.0%
!!! TAACTTAATTTTCTAATGGA+AGG - Chr3:4932610-4932629 None:intergenic 20.0%
!!! TCAAGCTTTATATATTTATG+AGG + Chr3:4933086-4933105 MsG0380011772.01.T01:CDS 20.0%
! AAGTTAGTGTTTATACTTTG+GGG + Chr3:4932731-4932750 MsG0380011772.01.T01:CDS 25.0%
! CAAAACAACTATGAACAAAT+AGG + Chr3:4933207-4933226 MsG0380011772.01.T01:CDS 25.0%
! GAAGTTAGTGTTTATACTTT+GGG + Chr3:4932730-4932749 MsG0380011772.01.T01:CDS 25.0%
! TGAAGTTAGTGTTTATACTT+TGG + Chr3:4932729-4932748 MsG0380011772.01.T01:CDS 25.0%
! TTTAGATGTTTGGATTATGA+AGG + Chr3:4932990-4933009 MsG0380011772.01.T01:CDS 25.0%
!!! AATATTAAAAGTACCAGTCT+TGG - Chr3:4933178-4933197 None:intergenic 25.0%
!!! CATGTTTTTCAATTAAGCTT+TGG + Chr3:4932656-4932675 MsG0380011772.01.T01:intron 25.0%
!!! CTGATATGTTTTTAGATGTT+TGG + Chr3:4932980-4932999 MsG0380011772.01.T01:CDS 25.0%
!!! GTTCTTATTCTTATTTTTGG+TGG + Chr3:4932581-4932600 MsG0380011772.01.T01:intron 25.0%
AATTAAGTTATTGCCTCCTT+TGG + Chr3:4932620-4932639 MsG0380011772.01.T01:intron 30.0%
TCTTTCTCTTGATTTAGTGA+AGG + Chr3:4932867-4932886 MsG0380011772.01.T01:CDS 30.0%
!!! TCATAGTTGTTTTGAAACTC+AGG - Chr3:4933201-4933220 None:intergenic 30.0%
AATGAACCACAAATCACTCT+TGG - Chr3:4932923-4932942 None:intergenic 35.0%
CACAAATCACTCTTGGAATT+AGG - Chr3:4932916-4932935 None:intergenic 35.0%
CATGAAATTCTAACAGCACT+TGG - Chr3:4933116-4933135 None:intergenic 35.0%
CTAATTCCAAGAGTGATTTG+TGG + Chr3:4932914-4932933 MsG0380011772.01.T01:CDS 35.0%
CTGTTAGAATTTCATGAGAC+GGG + Chr3:4933120-4933139 MsG0380011772.01.T01:CDS 35.0%
GCTGTTAGAATTTCATGAGA+CGG + Chr3:4933119-4933138 MsG0380011772.01.T01:CDS 35.0%
GTTGTTTATGATTCCAAGAC+TGG + Chr3:4933162-4933181 MsG0380011772.01.T01:CDS 35.0%
GTTTGGATTATGAAGGAGTA+CGG + Chr3:4932997-4933016 MsG0380011772.01.T01:CDS 35.0%
! AAGAGTGATTTGTGGTTCAT+TGG + Chr3:4932922-4932941 MsG0380011772.01.T01:CDS 35.0%
! ATACTTTGGGGACTGATTAT+TGG + Chr3:4932743-4932762 MsG0380011772.01.T01:CDS 35.0%
! ATGTCGAGTGAATTTTCCAA+AGG - Chr3:4932639-4932658 None:intergenic 35.0%
! TGATTTGTGGTTCATTGGTT+TGG + Chr3:4932927-4932946 MsG0380011772.01.T01:CDS 35.0%
AGTACGGAAATCAAGAGTCT+TGG + Chr3:4933013-4933032 MsG0380011772.01.T01:CDS 40.0%
GATCTTGTATGTCAGGAACA+CGG - Chr3:4933050-4933069 None:intergenic 40.0%
GATGGCATGTGGCATTATAT+GGG - Chr3:4932786-4932805 None:intergenic 40.0%
GTTCCTGACATACAAGATCA+GGG + Chr3:4933051-4933070 MsG0380011772.01.T01:CDS 40.0%
TGATGGCATGTGGCATTATA+TGG - Chr3:4932787-4932806 None:intergenic 40.0%
TGTTCCTGACATACAAGATC+AGG + Chr3:4933050-4933069 MsG0380011772.01.T01:CDS 40.0%
TTAGAATTTCATGAGACGGG+AGG + Chr3:4933123-4933142 MsG0380011772.01.T01:CDS 40.0%
TTGTCAGTGGCACTGTTAAT+TGG + Chr3:4932818-4932837 MsG0380011772.01.T01:CDS 40.0%
! TCGAGTGAATTTTCCAAAGG+AGG - Chr3:4932636-4932655 None:intergenic 40.0%
! TGACAAATGTTGCGGATTGA+TGG - Chr3:4932804-4932823 None:intergenic 40.0%
AGACCCTGATCTTGTATGTC+AGG - Chr3:4933057-4933076 None:intergenic 45.0%
ATACAAGATCAGGGTCTCGA+AGG + Chr3:4933060-4933079 MsG0380011772.01.T01:CDS 45.0%
CAATCCGCAACATTTGTCAG+TGG + Chr3:4932805-4932824 MsG0380011772.01.T01:CDS 45.0%
CAGTGGCACTGTTAATTGGT+TGG + Chr3:4932822-4932841 MsG0380011772.01.T01:CDS 45.0%
GGGAGGTGGAAAAATGAAGT+TGG + Chr3:4933140-4933159 MsG0380011772.01.T01:CDS 45.0%
GTGGCATTATATGGGATGTC+TGG - Chr3:4932778-4932797 None:intergenic 45.0%
! AGTGCCACTGACAAATGTTG+CGG - Chr3:4932812-4932831 None:intergenic 45.0%
! TCCGATGGCACATCTTTTGT+GGG + Chr3:4932544-4932563 MsG0380011772.01.T01:CDS 45.0%
! TTCCGATGGCACATCTTTTG+TGG + Chr3:4932543-4932562 MsG0380011772.01.T01:CDS 45.0%
!! GTGGTTCATTGGTTTGGTGA+GGG + Chr3:4932933-4932952 MsG0380011772.01.T01:CDS 45.0%
!! TGTGGTTCATTGGTTTGGTG+AGG + Chr3:4932932-4932951 MsG0380011772.01.T01:CDS 45.0%
GAATTTCATGAGACGGGAGG+TGG + Chr3:4933126-4933145 MsG0380011772.01.T01:CDS 50.0%
GCCCACAAAAGATGTGCCAT+CGG - Chr3:4932548-4932567 None:intergenic 50.0%
! TGTTGCGGATTGATGGCATG+TGG - Chr3:4932797-4932816 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 4932535 4933266 4932535 ID=MsG0380011772.01;Name=MsG0380011772.01
Chr3 mRNA 4932535 4933266 4932535 ID=MsG0380011772.01.T01;Parent=MsG0380011772.01;Name=MsG0380011772.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|201
Chr3 exon 4932535 4932580 4932535 ID=MsG0380011772.01.T01:exon:2349;Parent=MsG0380011772.01.T01
Chr3 exon 4932707 4933266 4932707 ID=MsG0380011772.01.T01:exon:2350;Parent=MsG0380011772.01.T01
Chr3 CDS 4932535 4932580 4932535 ID=MsG0380011772.01.T01:cds;Parent=MsG0380011772.01.T01
Chr3 CDS 4932707 4933266 4932707 ID=MsG0380011772.01.T01:cds;Parent=MsG0380011772.01.T01
Gene Sequence

>MsG0380011772.01.T01

ATGTGCTCTTCCGATGGCACATCTTTTGTGGGCAAGCTCGATGTTAGTCTTGTTGTTTCTACCAAAAATGAAGTTAGTGTTTATACTTTGGGGACTGATTATTGGCTAAGAATACCAGACATCCCATATAATGCCACATGCCATCAATCCGCAACATTTGTCAGTGGCACTGTTAATTGGTTGGCAAGCAATGATTCACATTTCATTCTTTCTCTTGATTTAGTGAAGGAGTCATATCAAAAACTTTTGCTACCTAATTCCAAGAGTGATTTGTGGTTCATTGGTTTGGTGAGGGATTGCTTGTGTGTCTCTGCAACTACTGATATGTTTTTAGATGTTTGGATTATGAAGGAGTACGGAAATCAAGAGTCTTGGACTAAATTGTACCGTGTTCCTGACATACAAGATCAGGGTCTCGAAGGCTATCAAGCTTTATATATTTATGAGGATGACCAAGTGCTGTTAGAATTTCATGAGACGGGAGGTGGAAAAATGAAGTTGGTTGTTTATGATTCCAAGACTGGTACTTTTAATATTCCTGAGTTTCAAAACAACTATGAACAAATAGGCTCAAATGTCTACATTGAGAGTTTGATATCACCTTAA
Protein sequence

>MsG0380011772.01.T01

MCSSDGTSFVGKLDVSLVVSTKNEVSVYTLGTDYWLRIPDIPYNATCHQSATFVSGTVNWLASNDSHFILSLDLVKESYQKLLLPNSKSDLWFIGLVRDCLCVSATTDMFLDVWIMKEYGNQESWTKLYRVPDIQDQGLEGYQALYIYEDDQVLLEFHETGGGKMKLVVYDSKTGTFNIPEFQNNYEQIGSNVYIESLISP*