AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012006.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012006.01.T01 MTR_3g011890 89.175 194 17 2 7 199 67 257 2.22e-127 361
MsG0380012006.01.T01 MTR_3g415610 84.507 142 16 2 67 207 13 149 1.78e-77 243
MsG0380012006.01.T01 MTR_8g041870 54.945 182 79 2 19 199 81 260 9.98e-69 219
MsG0380012006.01.T01 MTR_8g041880 54.945 182 78 3 19 199 81 259 2.60e-67 219
MsG0380012006.01.T01 MTR_8g041650 57.527 186 73 4 19 202 84 265 1.00e-66 218
MsG0380012006.01.T01 MTR_8g041660 54.098 183 79 3 19 198 80 260 8.61e-64 208
MsG0380012006.01.T01 MTR_8g041660 54.098 183 79 3 19 198 80 260 7.50e-63 207
MsG0380012006.01.T01 MTR_8g041710 51.630 184 83 4 19 199 77 257 8.36e-63 207
MsG0380012006.01.T01 MTR_8g041910 50.549 182 87 2 19 199 89 268 3.47e-60 201
MsG0380012006.01.T01 MTR_8g041890 51.099 182 86 2 19 199 81 260 2.25e-58 196
MsG0380012006.01.T01 MTR_8g041690 45.109 184 95 4 19 199 80 260 5.97e-51 176
MsG0380012006.01.T01 MTR_3g011910 37.306 193 117 3 7 197 79 269 8.86e-40 144
MsG0380012006.01.T01 MTR_3g011910 37.306 193 117 3 7 197 53 243 3.52e-39 143
MsG0380012006.01.T01 MTR_2g088980 35.676 185 114 4 5 187 64 245 4.46e-34 123
MsG0380012006.01.T01 MTR_2g088930 34.239 184 118 2 5 187 64 245 2.37e-33 121
MsG0380012006.01.T01 MTR_7g052020 36.111 180 110 4 19 195 70 247 1.31e-30 114
MsG0380012006.01.T01 MTR_5g005480 34.659 176 107 3 18 188 79 251 1.28e-29 116
MsG0380012006.01.T01 MTR_1g105820 32.432 185 122 2 18 199 79 263 1.35e-28 113
MsG0380012006.01.T01 MTR_1g105615 30.890 191 124 5 19 205 83 269 1.29e-27 110
MsG0380012006.01.T01 MTR_6g055960 33.871 186 112 4 19 198 72 252 2.88e-27 110
MsG0380012006.01.T01 MTR_6g055960 27.473 182 126 4 9 186 304 483 5.59e-21 91.7
MsG0380012006.01.T01 MTR_6g463830 36.702 188 106 7 19 198 77 259 3.39e-27 109
MsG0380012006.01.T01 MTR_6g463830 27.551 196 133 5 9 197 311 504 6.88e-20 88.6
MsG0380012006.01.T01 MTR_1g105640 35.326 184 109 6 19 194 79 260 3.55e-27 109
MsG0380012006.01.T01 MTR_1g105640 35.326 184 109 6 19 194 79 260 3.71e-27 109
MsG0380012006.01.T01 MTR_1g105640 35.326 184 109 6 19 194 79 260 3.80e-27 109
MsG0380012006.01.T01 MTR_3g415620 88.679 53 6 0 18 70 155 207 4.35e-27 103
MsG0380012006.01.T01 MTR_0007s0020 34.536 194 103 4 19 195 74 260 1.15e-26 108
MsG0380012006.01.T01 MTR_0007s0020 29.101 189 127 6 19 203 323 508 1.80e-21 93.2
MsG0380012006.01.T01 MTR_1g104890 34.043 188 117 5 18 200 89 274 1.38e-26 105
MsG0380012006.01.T01 MTR_1g021610 33.523 176 107 5 19 187 81 253 1.66e-26 107
MsG0380012006.01.T01 MTR_1g105755 33.908 174 109 3 19 189 80 250 1.80e-26 107
MsG0380012006.01.T01 MTR_5g005530 33.721 172 108 4 18 186 79 247 2.79e-26 107
MsG0380012006.01.T01 MTR_1g104890 34.043 188 117 5 18 200 89 274 5.34e-26 105
MsG0380012006.01.T01 MTR_1g105800 32.065 184 119 3 19 199 81 261 7.01e-26 105
MsG0380012006.01.T01 MTR_1g104890 34.043 188 117 5 18 200 89 274 1.28e-25 105
MsG0380012006.01.T01 MTR_2g010730 32.143 196 115 5 21 207 84 270 1.56e-25 101
MsG0380012006.01.T01 MTR_1g104890 34.043 188 117 5 18 200 89 274 1.71e-25 104
MsG0380012006.01.T01 MTR_2g010710 29.570 186 116 4 21 200 79 255 4.38e-25 100
MsG0380012006.01.T01 MTR_5g068260 32.184 174 109 5 19 187 76 245 5.78e-25 103
MsG0380012006.01.T01 MTR_1g105650 34.615 182 101 5 17 187 80 254 6.28e-25 103
MsG0380012006.01.T01 MTR_5g068250 33.721 172 109 4 19 187 81 250 1.02e-24 102
MsG0380012006.01.T01 MTR_5g005450 30.233 172 113 3 18 186 78 245 1.10e-24 102
MsG0380012006.01.T01 MTR_1g105655 32.461 191 117 5 23 206 89 274 2.30e-24 101
MsG0380012006.01.T01 MTR_5g005520 31.319 182 117 7 18 193 74 253 9.58e-24 99.8
MsG0380012006.01.T01 MTR_6g463660 31.373 204 125 6 19 209 79 280 1.04e-23 99.4
MsG0380012006.01.T01 MTR_6g463660 28.962 183 124 4 8 186 310 490 1.40e-21 93.2
MsG0380012006.01.T01 MTR_6g057680 31.373 204 125 6 19 209 79 280 1.04e-23 99.4
MsG0380012006.01.T01 MTR_6g057680 28.962 183 124 4 8 186 310 490 1.40e-21 93.2
MsG0380012006.01.T01 MTR_5g005520 31.319 182 117 7 18 193 79 258 1.51e-23 99.4
MsG0380012006.01.T01 MTR_1g021632 33.333 192 115 6 18 203 78 262 2.25e-23 98.6
MsG0380012006.01.T01 MTR_6g057750 28.824 170 117 2 19 186 85 252 6.17e-23 97.4
MsG0380012006.01.T01 MTR_6g057750 27.128 188 121 6 19 196 330 511 1.12e-16 79.3
MsG0380012006.01.T01 MTR_6g463700 28.824 170 117 2 19 186 85 252 6.51e-23 97.4
MsG0380012006.01.T01 MTR_5g068190 32.386 176 113 4 19 188 74 249 1.28e-22 96.7
MsG0380012006.01.T01 MTR_1g105725 31.053 190 107 5 18 191 91 272 1.32e-22 96.3
MsG0380012006.01.T01 MTR_1g105750 30.978 184 124 2 19 199 81 264 2.19e-22 95.9
MsG0380012006.01.T01 MTR_1g105840 30.994 171 116 2 27 195 77 247 2.62e-22 95.5
MsG0380012006.01.T01 MTR_1g021638 34.091 176 110 5 18 187 78 253 3.35e-22 93.2
MsG0380012006.01.T01 MTR_1g021635 31.638 177 115 4 19 189 79 255 2.16e-21 92.8
MsG0380012006.01.T01 MTR_6g463400 30.168 179 110 5 20 189 80 252 2.36e-21 92.4
MsG0380012006.01.T01 MTR_6g463400 28.962 183 124 4 8 186 310 490 8.24e-21 90.9
MsG0380012006.01.T01 MTR_8g028065 28.649 185 127 2 19 203 80 259 2.91e-21 92.8
MsG0380012006.01.T01 MTR_8g028065 27.528 178 113 5 20 187 317 488 7.14e-18 82.8
MsG0380012006.01.T01 MTR_1g105850 29.213 178 115 6 18 187 84 258 3.22e-21 90.5
MsG0380012006.01.T01 MTR_6g463710 29.319 191 111 5 9 186 312 491 7.47e-21 91.3
MsG0380012006.01.T01 MTR_6g463710 32.967 182 114 3 18 193 79 258 8.86e-21 91.3
MsG0380012006.01.T01 MTR_6g057770 29.319 191 111 5 9 186 312 491 7.54e-21 91.3
MsG0380012006.01.T01 MTR_6g057770 32.967 182 114 3 18 193 79 258 8.61e-21 91.3
MsG0380012006.01.T01 MTR_1g021642 33.533 167 102 7 27 186 102 266 9.28e-21 91.3
MsG0380012006.01.T01 MTR_1g105860 28.342 187 123 5 19 197 99 282 2.76e-20 87.4
MsG0380012006.01.T01 MTR_1g105860 28.342 187 123 5 19 197 82 265 3.26e-20 87.0
MsG0380012006.01.T01 MTR_1g105885 29.762 168 109 3 27 187 86 251 3.29e-19 84.0
MsG0380012006.01.T01 MTR_0341s0010 30.601 183 115 6 18 189 78 259 1.08e-18 83.6
MsG0380012006.01.T01 MTR_1g021630 30.601 183 115 6 18 189 78 259 2.69e-18 84.0
MsG0380012006.01.T01 MTR_1g105710 27.778 198 127 7 19 204 72 265 2.88e-18 84.0
MsG0380012006.01.T01 MTR_1g105830 27.717 184 117 3 19 200 95 264 4.04e-18 81.6
MsG0380012006.01.T01 MTR_6g053000 30.588 170 113 4 19 186 77 243 6.40e-18 82.8
MsG0380012006.01.T01 MTR_1g105600 31.111 180 111 6 18 186 332 509 1.12e-17 82.0
MsG0380012006.01.T01 MTR_1g105595 28.108 185 128 4 18 199 84 266 2.13e-17 81.3
MsG0380012006.01.T01 MTR_1g105595 28.108 185 128 4 18 199 84 266 2.84e-17 80.9
MsG0380012006.01.T01 MTR_2g010720 28.723 188 115 8 21 201 144 319 4.43e-17 79.3
MsG0380012006.01.T01 MTR_1g105700 28.571 196 120 7 19 200 77 266 2.03e-16 78.6
MsG0380012006.01.T01 MTR_1g105585 29.885 174 115 5 18 187 84 254 5.58e-16 77.0
MsG0380012006.01.T01 MTR_3g079850 29.730 185 112 8 19 187 80 262 3.36e-15 74.7
MsG0380012006.01.T01 MTR_3g079850 29.730 185 112 8 19 187 80 262 3.48e-15 74.7
MsG0380012006.01.T01 MTR_5g065130 25.000 192 129 5 19 198 68 256 3.66e-14 71.6
MsG0380012006.01.T01 MTR_7g098410 27.607 163 109 2 19 180 74 228 4.43e-14 70.5
MsG0380012006.01.T01 MTR_3g011930 31.193 109 73 1 87 195 5 111 4.53e-14 71.2
MsG0380012006.01.T01 MTR_0341s0020 27.119 177 98 6 18 189 78 228 1.86e-11 63.9
MsG0380012006.01.T01 MTR_8g041670 60.976 41 16 0 148 188 14 54 2.47e-11 63.2
MsG0380012006.01.T01 MTR_0074s0060 25.926 189 105 6 19 197 77 240 5.04e-11 62.4
MsG0380012006.01.T01 MTR_5g068290 31.469 143 83 6 65 201 1 134 5.79e-11 62.0
MsG0380012006.01.T01 MTR_6g057440 41.772 79 42 3 19 93 77 155 8.80e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012006.01.T01 AT4G05200 34.104 173 107 4 21 189 82 251 7.39e-31 120
MsG0380012006.01.T01 AT4G05200 34.104 173 107 4 21 189 100 269 8.72e-31 120
MsG0380012006.01.T01 AT3G22060 35.882 170 103 4 21 187 84 250 1.48e-30 114
MsG0380012006.01.T01 AT4G23180 34.302 172 103 5 19 186 88 253 1.78e-28 112
MsG0380012006.01.T01 AT4G23180 34.302 172 103 5 19 186 88 253 2.20e-28 112
MsG0380012006.01.T01 AT4G23180 34.302 172 103 5 19 186 88 253 2.67e-28 112
MsG0380012006.01.T01 AT3G58310 34.503 171 105 4 19 185 86 253 3.57e-27 105
MsG0380012006.01.T01 AT4G38830 31.429 175 109 4 19 186 75 245 4.29e-25 103
MsG0380012006.01.T01 AT4G21410 31.019 216 132 6 19 221 80 291 2.05e-24 101
MsG0380012006.01.T01 AT5G48540 33.721 172 108 4 19 186 72 241 2.16e-24 98.2
MsG0380012006.01.T01 AT4G21410 31.019 216 132 6 19 221 80 291 2.96e-24 101
MsG0380012006.01.T01 AT4G23310 32.240 183 113 6 19 197 190 365 6.02e-23 97.4
MsG0380012006.01.T01 AT4G23310 25.731 171 112 3 19 182 79 241 1.80e-11 63.9
MsG0380012006.01.T01 AT4G23310 32.240 183 113 6 19 197 190 365 6.66e-23 97.4
MsG0380012006.01.T01 AT4G23310 25.731 171 112 3 19 182 79 241 2.06e-11 63.9
MsG0380012006.01.T01 AT4G23280 31.551 187 114 6 19 197 77 257 7.62e-23 97.1
MsG0380012006.01.T01 AT2G31620 32.632 190 107 7 7 185 73 252 7.77e-23 94.0
MsG0380012006.01.T01 AT4G23280 31.977 172 107 5 19 186 77 242 9.23e-23 97.1
MsG0380012006.01.T01 AT4G23280 31.977 172 107 5 19 186 77 242 1.03e-22 96.7
MsG0380012006.01.T01 AT4G23270 32.558 172 106 5 19 186 75 240 4.57e-22 94.7
MsG0380012006.01.T01 AT4G23270 32.558 172 106 5 19 186 75 240 4.57e-22 94.7
MsG0380012006.01.T01 AT4G23270 32.558 172 106 5 19 186 75 240 5.33e-22 94.7
MsG0380012006.01.T01 AT4G21400 29.016 193 119 7 19 202 82 265 5.65e-22 94.4
MsG0380012006.01.T01 AT4G21400 29.016 193 119 7 19 202 82 265 5.65e-22 94.4
MsG0380012006.01.T01 AT4G21400 29.016 193 119 7 19 202 82 265 5.65e-22 94.4
MsG0380012006.01.T01 AT4G21400 28.796 191 122 7 19 202 56 239 7.27e-22 94.4
MsG0380012006.01.T01 AT4G21400 29.016 193 119 7 19 202 82 265 7.42e-22 94.0
MsG0380012006.01.T01 AT4G21400 28.796 191 122 7 19 202 82 265 7.67e-22 94.4
MsG0380012006.01.T01 AT4G21400 28.796 191 122 7 19 202 82 265 8.22e-22 94.4
MsG0380012006.01.T01 AT4G23230 29.444 180 116 4 19 193 75 248 3.97e-21 92.0
MsG0380012006.01.T01 AT3G21990 31.818 176 107 5 21 187 84 255 4.04e-21 89.4
MsG0380012006.01.T01 AT3G21960 28.402 169 115 3 25 187 93 261 4.71e-21 89.4
MsG0380012006.01.T01 AT5G41290 30.636 173 108 5 19 183 76 244 5.16e-21 89.7
MsG0380012006.01.T01 AT4G23160 32.086 187 115 6 19 199 674 854 2.21e-20 90.1
MsG0380012006.01.T01 AT4G23160 32.086 187 115 6 19 199 674 854 2.21e-20 90.1
MsG0380012006.01.T01 AT4G20670 28.649 185 121 3 21 197 84 265 2.45e-20 87.4
MsG0380012006.01.T01 AT4G23160 32.086 187 115 6 19 199 94 274 2.96e-20 89.7
MsG0380012006.01.T01 AT1G61750 32.727 165 105 5 20 180 78 240 3.78e-20 88.2
MsG0380012006.01.T01 AT1G63570 30.994 171 112 4 19 184 74 243 6.47e-20 86.7
MsG0380012006.01.T01 AT1G63570 30.994 171 112 4 19 184 74 243 6.47e-20 86.7
MsG0380012006.01.T01 AT5G41300 30.952 168 108 4 19 180 80 245 6.75e-20 86.7
MsG0380012006.01.T01 AT4G20640 29.143 175 113 3 21 187 84 255 7.90e-20 85.9
MsG0380012006.01.T01 AT4G20580 29.143 175 113 3 21 187 84 255 7.90e-20 85.9
MsG0380012006.01.T01 AT4G20610 29.143 175 113 3 21 187 84 255 7.90e-20 85.9
MsG0380012006.01.T01 AT4G20630 29.143 175 113 3 21 187 84 255 7.90e-20 85.9
MsG0380012006.01.T01 AT4G20620 29.143 175 113 3 21 187 84 255 7.90e-20 85.9
MsG0380012006.01.T01 AT4G20590 29.143 175 113 3 21 187 84 255 7.90e-20 85.9
MsG0380012006.01.T01 AT4G20600 29.143 175 113 3 21 187 84 255 7.90e-20 85.9
MsG0380012006.01.T01 AT5G41280 30.178 169 110 4 19 181 77 243 8.76e-20 86.3
MsG0380012006.01.T01 AT1G63570 30.994 171 112 4 19 184 127 296 8.93e-20 87.0
MsG0380012006.01.T01 AT4G20570 28.902 173 112 3 21 185 98 267 9.49e-20 85.9
MsG0380012006.01.T01 AT4G20560 28.902 173 112 3 21 185 98 267 9.49e-20 85.9
MsG0380012006.01.T01 AT4G20645 28.902 173 112 3 21 185 98 267 9.49e-20 85.9
MsG0380012006.01.T01 AT4G20550 28.902 173 112 3 21 185 98 267 9.49e-20 85.9
MsG0380012006.01.T01 AT4G20530 28.902 173 112 3 21 185 98 267 9.49e-20 85.9
MsG0380012006.01.T01 AT4G20540 28.902 173 112 3 21 185 98 267 9.49e-20 85.9
MsG0380012006.01.T01 AT4G23170 29.609 179 113 4 19 190 77 249 1.45e-19 85.1
MsG0380012006.01.T01 AT3G29050 27.551 196 115 5 21 195 91 280 4.53e-19 84.3
MsG0380012006.01.T01 AT3G21930 32.402 179 106 5 19 185 92 267 4.63e-19 84.0
MsG0380012006.01.T01 AT4G23150 30.270 185 106 5 18 190 76 249 6.93e-19 85.5
MsG0380012006.01.T01 AT3G21920 32.402 179 106 5 19 185 114 289 9.69e-19 83.6
MsG0380012006.01.T01 AT3G21920 32.402 179 106 5 19 185 92 267 1.01e-18 83.2
MsG0380012006.01.T01 AT4G20680 29.381 194 112 6 19 194 87 273 7.78e-18 80.5
MsG0380012006.01.T01 AT3G21970 32.203 177 107 5 19 185 88 261 1.39e-17 79.7
MsG0380012006.01.T01 AT1G63550 28.402 169 111 3 19 180 78 243 2.17e-17 80.1
MsG0380012006.01.T01 AT3G22000 30.412 194 110 7 19 194 87 273 2.44e-17 79.3
MsG0380012006.01.T01 AT4G21230 28.736 174 117 4 27 196 83 253 2.64e-17 80.9
MsG0380012006.01.T01 AT4G23140 30.857 175 108 5 19 186 78 246 2.82e-17 80.9
MsG0380012006.01.T01 AT4G23140 30.857 175 108 5 19 186 78 246 3.04e-17 80.9
MsG0380012006.01.T01 AT4G23130 31.818 176 109 5 19 189 78 247 3.45e-17 80.9
MsG0380012006.01.T01 AT4G23130 31.818 176 109 5 19 189 78 247 3.64e-17 80.5
MsG0380012006.01.T01 AT4G23130 31.818 176 109 5 19 189 78 247 3.69e-17 80.5
MsG0380012006.01.T01 AT3G22050 28.736 174 115 5 19 185 84 255 1.44e-16 77.0
MsG0380012006.01.T01 AT3G22040 30.726 179 99 6 24 187 88 256 1.72e-16 76.6
MsG0380012006.01.T01 AT3G45860 29.775 178 112 6 19 190 81 251 2.41e-16 78.2
MsG0380012006.01.T01 AT3G21910 31.053 190 113 8 19 195 88 272 3.64e-16 76.3
MsG0380012006.01.T01 AT3G60720 28.000 175 114 4 20 193 82 245 5.16e-16 75.1
MsG0380012006.01.T01 AT3G22010 28.788 198 109 8 7 185 76 260 1.17e-15 74.3
MsG0380012006.01.T01 AT3G60720 27.907 172 112 4 20 190 82 242 1.19e-15 74.7
MsG0380012006.01.T01 AT4G11460 26.702 191 105 5 19 185 71 250 1.39e-15 75.9
MsG0380012006.01.T01 AT4G11460 26.702 191 105 5 19 185 71 250 1.40e-15 75.9
MsG0380012006.01.T01 AT3G60720 27.907 172 112 4 20 190 82 242 1.49e-15 74.7
MsG0380012006.01.T01 AT3G21940 31.111 180 100 7 29 187 3 179 1.80e-15 72.4
MsG0380012006.01.T01 AT3G29040 29.310 174 113 5 19 185 82 252 8.24e-15 72.0
MsG0380012006.01.T01 AT1G63600 28.655 171 113 4 19 181 83 252 1.08e-14 72.4
MsG0380012006.01.T01 AT4G11460 27.429 175 92 5 35 185 12 175 1.41e-14 73.2
MsG0380012006.01.T01 AT4G23240 27.136 199 132 7 19 207 72 267 1.53e-14 72.8
MsG0380012006.01.T01 AT1G63590 28.824 170 114 5 19 181 75 244 1.88e-14 71.2
MsG0380012006.01.T01 AT4G11530 29.560 159 97 5 36 181 86 242 3.78e-14 71.6
MsG0380012006.01.T01 AT4G23220 28.571 189 128 5 19 201 68 255 1.01e-13 70.5
MsG0380012006.01.T01 AT4G11521 26.415 159 106 3 36 185 85 241 8.73e-13 66.6
MsG0380012006.01.T01 AT3G21900 27.225 191 121 6 2 185 68 247 1.54e-12 65.9
MsG0380012006.01.T01 AT1G70690 28.902 173 107 5 11 180 73 232 2.48e-12 65.5
MsG0380012006.01.T01 AT4G21410 30.000 170 102 6 65 221 1 166 4.68e-12 65.5
MsG0380012006.01.T01 AT4G23210 28.351 194 118 7 19 193 71 262 4.73e-12 65.5
MsG0380012006.01.T01 AT1G63580 24.432 176 114 3 19 181 68 237 5.84e-12 64.3
MsG0380012006.01.T01 AT4G23210 28.351 194 118 7 19 193 71 262 6.36e-12 65.1
MsG0380012006.01.T01 AT4G23190 28.571 175 115 5 21 185 74 248 8.51e-12 64.7
MsG0380012006.01.T01 AT4G23210 28.351 194 118 7 19 193 71 262 9.68e-12 64.7
MsG0380012006.01.T01 AT4G23210 28.351 194 118 7 19 193 71 262 9.68e-12 64.7
MsG0380012006.01.T01 AT4G23210 28.351 194 118 7 19 193 71 262 9.76e-12 64.7
MsG0380012006.01.T01 AT4G23190 28.814 177 112 6 21 185 74 248 1.02e-11 64.7
MsG0380012006.01.T01 AT4G23300 26.396 197 127 4 19 199 71 265 2.30e-11 63.5
MsG0380012006.01.T01 AT3G21940 29.167 192 104 9 19 187 92 274 2.42e-11 62.4
MsG0380012006.01.T01 AT4G23190 28.659 164 107 5 32 185 7 170 6.25e-11 62.4
MsG0380012006.01.T01 AT4G23190 28.659 164 107 5 32 185 7 170 6.25e-11 62.4

Find 46 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCATTGCATACAACTTAT+TGG 0.213123 3:-9434455 None:intergenic
GATTACATTGTTTGTCATTA+AGG 0.244483 3:-9434540 None:intergenic
CAGATGAATGGGAAAGGTTT+GGG 0.326981 3:+9433864 None:intergenic
GAATCATACAAACCTGGATT+TGG 0.328307 3:-9434703 None:intergenic
TTTAATAAGTGAAGCTACTT+TGG 0.335015 3:+9434429 MsG0380012006.01.T01:CDS
CACAAACTCTAGAAGAAAAT+AGG 0.336468 3:-9435750 None:intergenic
GGGAAAGGTTTGGGGCTGGG+TGG 0.337424 3:+9433873 MsG0380012006.01.T01:CDS
ATAACAGCTGAAGCTCTATT+AGG 0.342092 3:-9434101 None:intergenic
AATGGGAAAGGTTTGGGGCT+GGG 0.343230 3:+9433870 MsG0380012006.01.T01:CDS
ATAGAGCTTCAGCTGTTATA+TGG 0.367320 3:+9434105 MsG0380012006.01.T01:CDS
GACCCTGGCCTTTGCCATGA+TGG 0.388372 3:-9434352 None:intergenic
ATGGATTGGTGCAGTGTAGT+AGG 0.390086 3:+9434515 MsG0380012006.01.T01:CDS
CAAACAATGTAATCAATGTT+TGG 0.403809 3:+9434549 MsG0380012006.01.T01:CDS
ACAGATGAATGGGAAAGGTT+TGG 0.407470 3:+9433863 None:intergenic
CCTCTGGTTGATGCTGTCTA+TGG 0.415611 3:+9433993 MsG0380012006.01.T01:CDS
ACCAGAGGCGGAGCTATGTA+TGG 0.423410 3:-9433978 None:intergenic
TCTGCTCCTTTGCCAAATCC+AGG 0.449840 3:+9434691 MsG0380012006.01.T01:CDS
GGAAAGGTTTGGGGCTGGGT+GGG 0.461661 3:+9433874 MsG0380012006.01.T01:CDS
TCAATGTTTGGAGGCTATGT+TGG 0.470199 3:+9434561 MsG0380012006.01.T01:CDS
TTAACGTGTAACAGATGAAT+GGG 0.490451 3:+9433853 None:intergenic
AATCCATCATGGCAAAGGCC+AGG 0.494932 3:+9434349 MsG0380012006.01.T01:CDS
GTTAACGTGTAACAGATGAA+TGG 0.495817 3:+9433852 None:intergenic
GAATGGGAAAGGTTTGGGGC+TGG 0.499793 3:+9433869 MsG0380012006.01.T01:CDS
CCCATACATAGCTCCGCCTC+TGG 0.512800 3:+9433977 MsG0380012006.01.T01:CDS
CAGAAGTTGCAGAAGTAAGT+TGG 0.514885 3:-9434672 None:intergenic
CAATAAGTTGTATGCAATGA+GGG 0.524170 3:+9434456 MsG0380012006.01.T01:CDS
TCATACTTTATCAAACAACT+TGG 0.533938 3:-9434634 None:intergenic
GATTGTAGAGGTGATGTCAC+AGG 0.560133 3:+9434023 MsG0380012006.01.T01:CDS
TGGATTGGTGCAGTGTAGTA+GGG 0.565311 3:+9434516 MsG0380012006.01.T01:CDS
ATCCATCATGGCAAAGGCCA+GGG 0.569785 3:+9434350 MsG0380012006.01.T01:CDS
TCAGCTTTATCAAGTTCTCG+TGG 0.573307 3:-9434391 None:intergenic
GACGAAGTTCCTAAATGCTG+TGG 0.573980 3:+9434583 MsG0380012006.01.T01:CDS
GCCATGATGGATTTGTTGTG+AGG 0.574968 3:-9434339 None:intergenic
ACCTCACAACAAATCCATCA+TGG 0.591319 3:+9434338 MsG0380012006.01.T01:CDS
CCAGAGGCGGAGCTATGTAT+GGG 0.594860 3:-9433977 None:intergenic
TACAAACCTGGATTTGGCAA+AGG 0.598161 3:-9434697 None:intergenic
CCAATAAGTTGTATGCAATG+AGG 0.600025 3:+9434455 MsG0380012006.01.T01:CDS
ACAATGTAATCAATGTTTGG+AGG 0.612800 3:+9434552 MsG0380012006.01.T01:CDS
GTGTAACAGATGAATGGGAA+AGG 0.619364 3:+9433858 None:intergenic
CAACAAATCCATCATGGCAA+AGG 0.619573 3:+9434344 MsG0380012006.01.T01:CDS
AGATGAATGGGAAAGGTTTG+GGG 0.625333 3:+9433865 None:intergenic
ACGAAGTTCCTAAATGCTGT+GGG 0.644645 3:+9434584 MsG0380012006.01.T01:CDS
TATGGTCTCTATGATTGTAG+AGG 0.653148 3:+9434011 MsG0380012006.01.T01:CDS
ACGAGAACTTGATAAAGCTG+AGG 0.674476 3:+9434393 MsG0380012006.01.T01:CDS
TAGACAGCATCAACCAGAGG+CGG 0.688697 3:-9433990 None:intergenic
CCATAGACAGCATCAACCAG+AGG 0.718225 3:-9433993 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATCTTTTTTATTTTTGA+GGG + Chr3:9434831-9434850 MsG0380012006.01.T01:intron 10.0%
!!! TAAAATCTTTTTTATTTTTG+AGG + Chr3:9434830-9434849 MsG0380012006.01.T01:intron 10.0%
!! AAAAAAAAGTTAATATTGAC+AGG - Chr3:9435650-9435669 None:intergenic 15.0%
!! AAAAAAATAAAATAAGCTGA+GGG + Chr3:9433905-9433924 MsG0380012006.01.T01:intron 15.0%
!! TATACTAAAGATGATAAATT+AGG + Chr3:9435097-9435116 MsG0380012006.01.T01:intron 15.0%
!! TGAAAAAATAATGTTCAAAA+TGG + Chr3:9435443-9435462 MsG0380012006.01.T01:intron 15.0%
!!! AAATCTTTTTTATTTTTGAG+GGG + Chr3:9434832-9434851 MsG0380012006.01.T01:intron 15.0%
!!! AATTTTTATCTTATTGTGTT+TGG - Chr3:9435315-9435334 None:intergenic 15.0%
!!! TACATATAATGACTTTTAAA+AGG + Chr3:9435500-9435519 MsG0380012006.01.T01:intron 15.0%
!!! TTTAATTTACTTCAAAACAA+AGG + Chr3:9435543-9435562 MsG0380012006.01.T01:intron 15.0%
!!! TTTTTTACGTTCTTTTTTTT+TGG + Chr3:9434909-9434928 MsG0380012006.01.T01:intron 15.0%
!! AATACTATATAAGATGTTGT+TGG - Chr3:9434173-9434192 None:intergenic 20.0%
!! CATTATGAACAATTTAATTG+TGG + Chr3:9435269-9435288 MsG0380012006.01.T01:intron 20.0%
!! GAAAAAAATAAAATAAGCTG+AGG + Chr3:9433904-9433923 MsG0380012006.01.T01:intron 20.0%
!! TATTGAAAAAAGATATGGAT+TGG + Chr3:9434501-9434520 MsG0380012006.01.T01:CDS 20.0%
!! TTATATGTATGTTGAGTTTA+AGG - Chr3:9435489-9435508 None:intergenic 20.0%
!!! AATCCATATCTTTTTTCAAT+AGG - Chr3:9434502-9434521 None:intergenic 20.0%
! AATAGTCATCTAATATAGGA+CGG + Chr3:9434252-9434271 MsG0380012006.01.T01:intron 25.0%
! AGGAACATTAACTATTATAC+TGG - Chr3:9435630-9435649 None:intergenic 25.0%
! AGTCCTATTGAAAAAAGATA+TGG + Chr3:9434496-9434515 MsG0380012006.01.T01:CDS 25.0%
! CAAACAATGTAATCAATGTT+TGG + Chr3:9434549-9434568 MsG0380012006.01.T01:CDS 25.0%
! CAAAGTTAAATATTTGCACA+TGG + Chr3:9435419-9435438 MsG0380012006.01.T01:intron 25.0%
! GATTACATTGTTTGTCATTA+AGG - Chr3:9434543-9434562 None:intergenic 25.0%
! GTCAAATAGTCATCTAATAT+AGG + Chr3:9434248-9434267 MsG0380012006.01.T01:intron 25.0%
! TCATACTTTATCAAACAACT+TGG - Chr3:9434637-9434656 None:intergenic 25.0%
! TTTAATAAGTGAAGCTACTT+TGG + Chr3:9434429-9434448 MsG0380012006.01.T01:CDS 25.0%
! TTTCTAATATGTCTTTGAGT+AGG + Chr3:9434293-9434312 MsG0380012006.01.T01:intron 25.0%
!!! TTGGTAATTTCACGAATTTT+GGG + Chr3:9433932-9433951 MsG0380012006.01.T01:intron 25.0%
!!! TTTAAGGTTTAGAAAGTGTT+AGG - Chr3:9435473-9435492 None:intergenic 25.0%
!!! TTTACGTTCTTTTTTTTTGG+TGG + Chr3:9434912-9434931 MsG0380012006.01.T01:intron 25.0%
AAAAAAGCTGAAAGACAGAT+AGG + Chr3:9435349-9435368 MsG0380012006.01.T01:intron 30.0%
AAAAGTGAATCATACAAACC+TGG - Chr3:9434712-9434731 None:intergenic 30.0%
ACAATGTAATCAATGTTTGG+AGG + Chr3:9434552-9434571 MsG0380012006.01.T01:CDS 30.0%
CAATAAGTTGTATGCAATGA+GGG + Chr3:9434456-9434475 MsG0380012006.01.T01:CDS 30.0%
TTGTGAGGTTTCCAAAAAAA+TGG - Chr3:9434327-9434346 None:intergenic 30.0%
! AAATCTGAATTTGAGTGTTG+TGG + Chr3:9435045-9435064 MsG0380012006.01.T01:intron 30.0%
! CACAAACTCTAGAAGAAAAT+AGG - Chr3:9435753-9435772 None:intergenic 30.0%
! TGGTAATTTCACGAATTTTG+GGG + Chr3:9433933-9433952 MsG0380012006.01.T01:intron 30.0%
!!! AGGTATTCCAACCATTTTTT+TGG + Chr3:9434313-9434332 MsG0380012006.01.T01:intron 30.0%
!!! GTTGGTAATTTCACGAATTT+TGG + Chr3:9433931-9433950 MsG0380012006.01.T01:intron 30.0%
AAATGCTGTGGGACAAAAAT+TGG + Chr3:9434595-9434614 MsG0380012006.01.T01:CDS 35.0%
ATAACAGCTGAAGCTCTATT+AGG - Chr3:9434104-9434123 None:intergenic 35.0%
ATAGAGCTTCAGCTGTTATA+TGG + Chr3:9434105-9434124 MsG0380012006.01.T01:CDS 35.0%
CCAATAAGTTGTATGCAATG+AGG + Chr3:9434455-9434474 MsG0380012006.01.T01:CDS 35.0%
CCTCATTGCATACAACTTAT+TGG - Chr3:9434458-9434477 None:intergenic 35.0%
CTGGCAAAAACTTTGTTGAT+TGG - Chr3:9435611-9435630 None:intergenic 35.0%
GAATCATACAAACCTGGATT+TGG - Chr3:9434706-9434725 None:intergenic 35.0%
GAGGTTTCCAAAAAAATGGT+TGG - Chr3:9434323-9434342 None:intergenic 35.0%
GATTGGTATTTACTTGTCGT+TGG - Chr3:9435594-9435613 None:intergenic 35.0%
TATGGTCTCTATGATTGTAG+AGG + Chr3:9434011-9434030 MsG0380012006.01.T01:CDS 35.0%
! AAAATAAGCTGAGGGTATGT+TGG + Chr3:9433913-9433932 MsG0380012006.01.T01:intron 35.0%
! ATTTTTGTCCCACAGCATTT+AGG - Chr3:9434595-9434614 None:intergenic 35.0%
! GGTAATTTCACGAATTTTGG+GGG + Chr3:9433934-9433953 MsG0380012006.01.T01:intron 35.0%
! GGTACAACTTTTGCATTTTC+AGG + Chr3:9434126-9434145 MsG0380012006.01.T01:CDS 35.0%
!!! TTAGTGATGTTTTTTGACCC+TGG - Chr3:9434370-9434389 None:intergenic 35.0%
ACCTCACAACAAATCCATCA+TGG + Chr3:9434338-9434357 MsG0380012006.01.T01:CDS 40.0%
ACGAAGTTCCTAAATGCTGT+GGG + Chr3:9434584-9434603 MsG0380012006.01.T01:CDS 40.0%
ACGAGAACTTGATAAAGCTG+AGG + Chr3:9434393-9434412 MsG0380012006.01.T01:CDS 40.0%
AGTCATCTAATATAGGACGG+AGG + Chr3:9434255-9434274 MsG0380012006.01.T01:intron 40.0%
CAACAAATCCATCATGGCAA+AGG + Chr3:9434344-9434363 MsG0380012006.01.T01:CDS 40.0%
CAGAAGTTGCAGAAGTAAGT+TGG - Chr3:9434675-9434694 None:intergenic 40.0%
GTCATCTAATATAGGACGGA+GGG + Chr3:9434256-9434275 MsG0380012006.01.T01:intron 40.0%
TACAAACCTGGATTTGGCAA+AGG - Chr3:9434700-9434719 None:intergenic 40.0%
TCAATGTTTGGAGGCTATGT+TGG + Chr3:9434561-9434580 MsG0380012006.01.T01:CDS 40.0%
! TCAGCTTTATCAAGTTCTCG+TGG - Chr3:9434394-9434413 None:intergenic 40.0%
!! AATTTCACGAATTTTGGGGG+TGG + Chr3:9433937-9433956 MsG0380012006.01.T01:intron 40.0%
!! ATTTCACGAATTTTGGGGGT+GGG + Chr3:9433938-9433957 MsG0380012006.01.T01:intron 40.0%
GACGAAGTTCCTAAATGCTG+TGG + Chr3:9434583-9434602 MsG0380012006.01.T01:CDS 45.0%
GATTGTAGAGGTGATGTCAC+AGG + Chr3:9434023-9434042 MsG0380012006.01.T01:CDS 45.0%
GCTGTGGGACAAAAATTGGT+TGG + Chr3:9434599-9434618 MsG0380012006.01.T01:CDS 45.0%
TGGGACAAAAATTGGTTGGC+AGG + Chr3:9434603-9434622 MsG0380012006.01.T01:CDS 45.0%
!! ATGGATTGGTGCAGTGTAGT+AGG + Chr3:9434515-9434534 MsG0380012006.01.T01:CDS 45.0%
!! GCCATGATGGATTTGTTGTG+AGG - Chr3:9434342-9434361 None:intergenic 45.0%
!! TGGATTGGTGCAGTGTAGTA+GGG + Chr3:9434516-9434535 MsG0380012006.01.T01:CDS 45.0%
!!! TTATAATAAGTTTTTTTTTA+GGG - Chr3:9435715-9435734 None:intergenic 5.0%
!!! TTTATAATAAGTTTTTTTTT+AGG - Chr3:9435716-9435735 None:intergenic 5.0%
AATCCATCATGGCAAAGGCC+AGG + Chr3:9434349-9434368 MsG0380012006.01.T01:CDS 50.0%
ATCCATCATGGCAAAGGCCA+GGG + Chr3:9434350-9434369 MsG0380012006.01.T01:CDS 50.0%
CCATAGACAGCATCAACCAG+AGG - Chr3:9433996-9434015 None:intergenic 50.0%
TAGACAGCATCAACCAGAGG+CGG - Chr3:9433993-9434012 None:intergenic 50.0%
TCTGCTCCTTTGCCAAATCC+AGG + Chr3:9434691-9434710 MsG0380012006.01.T01:CDS 50.0%
! AATGGGAAAGGTTTGGGGCT+GGG + Chr3:9433870-9433889 MsG0380012006.01.T01:CDS 50.0%
! AGCTATGTATGGGCTGAGGT+GGG - Chr3:9433970-9433989 None:intergenic 50.0%
! CCTCTGGTTGATGCTGTCTA+TGG + Chr3:9433993-9434012 MsG0380012006.01.T01:CDS 50.0%
ACCAGAGGCGGAGCTATGTA+TGG - Chr3:9433981-9434000 None:intergenic 55.0%
CCAGAGGCGGAGCTATGTAT+GGG - Chr3:9433980-9433999 None:intergenic 55.0%
! GAATGGGAAAGGTTTGGGGC+TGG + Chr3:9433869-9433888 MsG0380012006.01.T01:CDS 55.0%
! GAGCTATGTATGGGCTGAGG+TGG - Chr3:9433971-9433990 None:intergenic 55.0%
!! TTGGGGCTGGGTGGGTTATT+TGG + Chr3:9433882-9433901 MsG0380012006.01.T01:intron 55.0%
CCCATACATAGCTCCGCCTC+TGG + Chr3:9433977-9433996 MsG0380012006.01.T01:CDS 60.0%
! GACCCTGGCCTTTGCCATGA+TGG - Chr3:9434355-9434374 None:intergenic 60.0%
! GCGGAGCTATGTATGGGCTG+AGG - Chr3:9433974-9433993 None:intergenic 60.0%
! GGAAAGGTTTGGGGCTGGGT+GGG + Chr3:9433874-9433893 MsG0380012006.01.T01:CDS 60.0%
! GGGAAAGGTTTGGGGCTGGG+TGG + Chr3:9433873-9433892 MsG0380012006.01.T01:CDS 65.0%
! GTATGGGCTGAGGTGGGCCA+CGG - Chr3:9433964-9433983 None:intergenic 65.0%
!! CGAATTTTGGGGGTGGGCCG+TGG + Chr3:9433944-9433963 MsG0380012006.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 9433867 9435796 9433867 ID=MsG0380012006.01;Name=MsG0380012006.01
Chr3 mRNA 9433867 9435796 9433867 ID=MsG0380012006.01.T01;Parent=MsG0380012006.01;Name=MsG0380012006.01.T01;_AED=0.53;_eAED=0.60;_QI=0|0|0|0.75|0.33|0.5|4|0|239
Chr3 exon 9433867 9433895 9433867 ID=MsG0380012006.01.T01:exon:2817;Parent=MsG0380012006.01.T01
Chr3 exon 9433977 9434147 9433977 ID=MsG0380012006.01.T01:exon:2818;Parent=MsG0380012006.01.T01
Chr3 exon 9434315 9434712 9434315 ID=MsG0380012006.01.T01:exon:2819;Parent=MsG0380012006.01.T01
Chr3 exon 9435675 9435796 9435675 ID=MsG0380012006.01.T01:exon:2820;Parent=MsG0380012006.01.T01
Chr3 CDS 9433867 9433895 9433867 ID=MsG0380012006.01.T01:cds;Parent=MsG0380012006.01.T01
Chr3 CDS 9433977 9434147 9433977 ID=MsG0380012006.01.T01:cds;Parent=MsG0380012006.01.T01
Chr3 CDS 9434315 9434712 9434315 ID=MsG0380012006.01.T01:cds;Parent=MsG0380012006.01.T01
Chr3 CDS 9435675 9435796 9435675 ID=MsG0380012006.01.T01:cds;Parent=MsG0380012006.01.T01
Gene Sequence

>MsG0380012006.01.T01

ATGAATGGGAAAGGTTTGGGGCTGGGTGGCCCATACATAGCTCCGCCTCTGGTTGATGCTGTCTATGGTCTCTATGATTGTAGAGGTGATGTCACAGGATCATTTTGTCAATTTTGTGTCTCTACTGCTGCTTCAGATGTTCTTCAACGCTGTCCTAATAGAGCTTCAGCTGTTATATGGTACAACTTTTGCATTTTCAGGTATTCCAACCATTTTTTTGGAAACCTCACAACAAATCCATCATGGCAAAGGCCAGGGTCAAAAAACATCACTAATCCACGAGAACTTGATAAAGCTGAGGATAATATGCAAAGTTTAATAAGTGAAGCTACTTTGGAGACCAATAAGTTGTATGCAATGAGGGAGTTCAATTTGAGTAATAGTCCTATTGAAAAAAGATATGGATTGGTGCAGTGTAGTAGGGACCTTAATGACAAACAATGTAATCAATGTTTGGAGGCTATGTTGGACGAAGTTCCTAAATGCTGTGGGACAAAAATTGGTTGGCAGGTTCTTGCTCCAAGTTGTTTGATAAAGTATGATGATTTTATGTTCTACCAACTTACTTCTGCAACTTCTGCTCCTTTGCCAAATCCAGAGTCAATATTAAGTTCAATAACTTATTATAAATATATCCCTAAAAAAAAACTTATTATAAATATAAATTATCATTCCTATTTTCTTCTAGAGTTTGTGTTATTTGAACAACCATTTATGTGA
Protein sequence

>MsG0380012006.01.T01

MNGKGLGLGGPYIAPPLVDAVYGLYDCRGDVTGSFCQFCVSTAASDVLQRCPNRASAVIWYNFCIFRYSNHFFGNLTTNPSWQRPGSKNITNPRELDKAEDNMQSLISEATLETNKLYAMREFNLSNSPIEKRYGLVQCSRDLNDKQCNQCLEAMLDEVPKCCGTKIGWQVLAPSCLIKYDDFMFYQLTSATSAPLPNPESILSSITYYKYIPKKKLIININYHSYFLLEFVLFEQPFM*