Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013061.01.T01 | XP_004488685.1 | 61.491 | 161 | 37 | 3 | 13 | 173 | 296 | 431 | 1.13E-46 | 169 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013061.01.T01 | A0A1S2XFR7 | 61.491 | 161 | 37 | 3 | 13 | 173 | 296 | 431 | 5.42e-47 | 169 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013061.01 | MsG0580027922.01 | 0.802616 | 5.154942e-49 | 2.536658e-46 |
| MsG0380013061.01 | MsG0880042454.01 | 0.810269 | 1.253509e-50 | 7.522386e-48 |
| MsG0380013061.01 | MsG0880045765.01 | 0.816144 | 6.434661e-52 | 4.523032e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013061.01.T01 | MTR_1g115250 | 42.424 | 165 | 73 | 2 | 13 | 176 | 292 | 435 | 1.07e-28 | 110 |
| MsG0380013061.01.T01 | MTR_7g094050 | 45.588 | 136 | 55 | 2 | 38 | 173 | 1 | 117 | 5.18e-26 | 97.1 |
| MsG0380013061.01.T01 | MTR_7g094030 | 45.255 | 137 | 56 | 2 | 37 | 173 | 331 | 448 | 2.02e-24 | 99.4 |
| MsG0380013061.01.T01 | MTR_2g082880 | 37.908 | 153 | 65 | 3 | 13 | 158 | 274 | 403 | 1.85e-21 | 90.5 |
| MsG0380013061.01.T01 | MTR_2g072710 | 34.454 | 119 | 58 | 2 | 39 | 157 | 66 | 164 | 1.03e-15 | 72.0 |
| MsG0380013061.01.T01 | MTR_2g082800 | 39.231 | 130 | 56 | 3 | 13 | 142 | 293 | 399 | 1.47e-14 | 71.2 |
| MsG0380013061.01.T01 | MTR_2g072730 | 35.398 | 113 | 53 | 1 | 45 | 157 | 330 | 422 | 1.59e-14 | 71.2 |
| MsG0380013061.01.T01 | MTR_1g025870 | 35.088 | 114 | 54 | 1 | 44 | 157 | 396 | 489 | 2.06e-14 | 70.9 |
| MsG0380013061.01.T01 | MTR_4g032845 | 36.283 | 113 | 52 | 1 | 45 | 157 | 316 | 408 | 2.47e-12 | 64.7 |
| MsG0380013061.01.T01 | MTR_2g072720 | 35.965 | 114 | 51 | 2 | 45 | 157 | 351 | 443 | 3.03e-12 | 64.3 |
| MsG0380013061.01.T01 | MTR_5g005700 | 30.539 | 167 | 87 | 3 | 9 | 167 | 323 | 468 | 4.30e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGTTAAATTTCGCTAAAT+TGG | 0.191519 | 3:-29689825 | MsG0380013061.01.T01:CDS |
| CATTCCTGACTTTCAAATAT+TGG | 0.191554 | 3:-29690492 | MsG0380013061.01.T01:CDS |
| TTAACATTTCCCATTTCTTC+TGG | 0.234575 | 3:+29689741 | None:intergenic |
| ATTCCTGACTTTCAAATATT+GGG | 0.284897 | 3:-29690491 | MsG0380013061.01.T01:CDS |
| CTCATGTTGTCCTAAGTTGT+TGG | 0.311473 | 3:-29689765 | MsG0380013061.01.T01:CDS |
| CTTTCAAATATTGGGTCATC+TGG | 0.346579 | 3:-29690483 | MsG0380013061.01.T01:CDS |
| GTAAAATTTGAAAGATTTGC+CGG | 0.347989 | 3:-29689857 | MsG0380013061.01.T01:CDS |
| AGTTGTTGGGCCAGAAGAAA+TGG | 0.370869 | 3:-29689751 | MsG0380013061.01.T01:CDS |
| GCTTTCAAGATCACCATGTT+TGG | 0.375297 | 3:-29690423 | MsG0380013061.01.T01:CDS |
| TCATGTTGTCCTAAGTTGTT+GGG | 0.383654 | 3:-29689764 | MsG0380013061.01.T01:CDS |
| TGACCCAATATTTGAAAGTC+AGG | 0.400469 | 3:+29690488 | None:intergenic |
| ATCACCATGTTTGGAGACTC+TGG | 0.444590 | 3:-29690414 | MsG0380013061.01.T01:CDS |
| GCTTAATTCTACATTTATGC+CGG | 0.449117 | 3:-29689906 | MsG0380013061.01.T01:CDS |
| CTTTCTATTTACCCTTAAAG+TGG | 0.467951 | 3:-29689879 | MsG0380013061.01.T01:CDS |
| TTCTTCTGGCCCAACAACTT+AGG | 0.478604 | 3:+29689755 | None:intergenic |
| ATTTAACTCATGTTTAGTAC+CGG | 0.485717 | 3:+29689838 | None:intergenic |
| GCTTTGTTTGCGTAGTAGAA+AGG | 0.492780 | 3:-29690789 | MsG0380013061.01.T01:intron |
| GTTGTTGGGCCAGAAGAAAT+GGG | 0.495328 | 3:-29689750 | MsG0380013061.01.T01:CDS |
| CTGGATCTCGACTTTGCAAC+CGG | 0.511629 | 3:-29690464 | MsG0380013061.01.T01:CDS |
| CTTAATTCTACATTTATGCC+GGG | 0.525933 | 3:-29689905 | MsG0380013061.01.T01:CDS |
| CCTCGTGAGCATAGCTCAGT+TGG | 0.543912 | 3:-29690245 | MsG0380013061.01.T01:intron |
| TGCTATATGGTTCAAGTAGG+AGG | 0.546606 | 3:-29690872 | None:intergenic |
| GATGAATAATGCTCAAGCCT+TGG | 0.546814 | 3:-29689801 | MsG0380013061.01.T01:CDS |
| AAAACCAGAGTCTCCAAACA+TGG | 0.617401 | 3:+29690410 | None:intergenic |
| CCAACTGAGCTATGCTCACG+AGG | 0.617839 | 3:+29690245 | None:intergenic |
| TTATGCTATATGGTTCAAGT+AGG | 0.633988 | 3:-29690875 | None:intergenic |
| AGGGTAAATAGAAAGCAACC+CGG | 0.732013 | 3:+29689887 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATATATGTATATATGTA+AGG | - | Chr3:29690357-29690376 | MsG0380013061.01.T01:intron | 10.0% |
| !! | TTAAATATTTATAGCAAACT+CGG | + | Chr3:29690441-29690460 | None:intergenic | 15.0% |
| !!! | TCACATAAACATTTTATTTA+CGG | - | Chr3:29689937-29689956 | MsG0380013061.01.T01:intron | 15.0% |
| !! | ATTTAATGTATTGCATAACA+TGG | + | Chr3:29690594-29690613 | None:intergenic | 20.0% |
| !! | ATTTGCTCATAGATAAATAT+AGG | - | Chr3:29690215-29690234 | MsG0380013061.01.T01:intron | 20.0% |
| !!! | ATATTTGTTTTACTCTACAT+GGG | - | Chr3:29689884-29689903 | MsG0380013061.01.T01:CDS | 20.0% |
| !!! | TATATTTGTTTTACTCTACA+TGG | - | Chr3:29689883-29689902 | MsG0380013061.01.T01:CDS | 20.0% |
| !!! | TTCAAATTTTACCACTTTAA+GGG | + | Chr3:29690701-29690720 | None:intergenic | 20.0% |
| !!! | TTTCAAATTTTACCACTTTA+AGG | + | Chr3:29690702-29690721 | None:intergenic | 20.0% |
| ! | ATATGTATATATGTAAGGTC+CGG | - | Chr3:29690362-29690381 | MsG0380013061.01.T01:intron | 25.0% |
| ! | ATTCCTGACTTTCAAATATT+GGG | - | Chr3:29690075-29690094 | MsG0380013061.01.T01:intron | 25.0% |
| ! | ATTTAACTCATGTTTAGTAC+CGG | + | Chr3:29690731-29690750 | None:intergenic | 25.0% |
| ! | GTAAAATTTGAAAGATTTGC+CGG | - | Chr3:29690709-29690728 | MsG0380013061.01.T01:intron | 25.0% |
| ! | TGAGTTAAATTTCGCTAAAT+TGG | - | Chr3:29690741-29690760 | MsG0380013061.01.T01:intron | 25.0% |
| !! | ATTTTTGTTCTTTCTCATCA+AGG | - | Chr3:29690020-29690039 | MsG0380013061.01.T01:intron | 25.0% |
| !! | CTTAAGTAGTTTTTGTCTTA+TGG | - | Chr3:29689856-29689875 | MsG0380013061.01.T01:CDS | 25.0% |
| !!! | ACTATACTTGTGTTTTTTTG+TGG | + | Chr3:29690405-29690424 | None:intergenic | 25.0% |
| !!! | TTTTTTTTTGACTGATCATC+AGG | - | Chr3:29690615-29690634 | MsG0380013061.01.T01:intron | 25.0% |
| AAGAACAAAAATTGTGTGTC+AGG | + | Chr3:29690012-29690031 | None:intergenic | 30.0% | |
| CATTCCTGACTTTCAAATAT+TGG | - | Chr3:29690074-29690093 | MsG0380013061.01.T01:intron | 30.0% | |
| CTTAATTCTACATTTATGCC+GGG | - | Chr3:29690661-29690680 | MsG0380013061.01.T01:intron | 30.0% | |
| CTTTCTATTTACCCTTAAAG+TGG | - | Chr3:29690687-29690706 | MsG0380013061.01.T01:intron | 30.0% | |
| GCTTAATTCTACATTTATGC+CGG | - | Chr3:29690660-29690679 | MsG0380013061.01.T01:intron | 30.0% | |
| TATGTATATATGTAAGGTCC+GGG | - | Chr3:29690363-29690382 | MsG0380013061.01.T01:intron | 30.0% | |
| TTAACATTTCCCATTTCTTC+TGG | + | Chr3:29690828-29690847 | None:intergenic | 30.0% | |
| ! | TGAGAAAGTAATCTTTTCCA+AGG | + | Chr3:29690785-29690804 | None:intergenic | 30.0% |
| !!! | ATACTTGTGTTTTTTTGTGG+TGG | + | Chr3:29690402-29690421 | None:intergenic | 30.0% |
| ATGTATATATGTAAGGTCCG+GGG | - | Chr3:29690364-29690383 | MsG0380013061.01.T01:intron | 35.0% | |
| CTTTCAAATATTGGGTCATC+TGG | - | Chr3:29690083-29690102 | MsG0380013061.01.T01:intron | 35.0% | |
| TCATGTTGTCCTAAGTTGTT+GGG | - | Chr3:29690802-29690821 | MsG0380013061.01.T01:CDS | 35.0% | |
| TGACCCAATATTTGAAAGTC+AGG | + | Chr3:29690081-29690100 | None:intergenic | 35.0% | |
| TGGTTCACAATCAGAGATAA+TGG | + | Chr3:29690574-29690593 | None:intergenic | 35.0% | |
| AAAACCAGAGTCTCCAAACA+TGG | + | Chr3:29690159-29690178 | None:intergenic | 40.0% | |
| AGGGTAAATAGAAAGCAACC+CGG | + | Chr3:29690682-29690701 | None:intergenic | 40.0% | |
| CATGCCAAACAAAAATTCGC+CGG | + | Chr3:29690124-29690143 | None:intergenic | 40.0% | |
| CTCATGTTGTCCTAAGTTGT+TGG | - | Chr3:29690801-29690820 | MsG0380013061.01.T01:CDS | 40.0% | |
| GATGAATAATGCTCAAGCCT+TGG | - | Chr3:29690765-29690784 | MsG0380013061.01.T01:intron | 40.0% | |
| GCTTTCAAGATCACCATGTT+TGG | - | Chr3:29690143-29690162 | MsG0380013061.01.T01:intron | 40.0% | |
| TTTGACTGATCATCAGGTAC+AGG | - | Chr3:29690621-29690640 | MsG0380013061.01.T01:intron | 40.0% | |
| !! | GCTTTGTTTGCGTAGTAGAA+AGG | - | Chr3:29689777-29689796 | MsG0380013061.01.T01:CDS | 40.0% |
| !!! | TTGTGTTTTTTTGTGGTGGC+CGG | + | Chr3:29690398-29690417 | None:intergenic | 40.0% |
| !!! | TTTGGAGACTCTGGTTTTGA+AGG | - | Chr3:29690161-29690180 | MsG0380013061.01.T01:intron | 40.0% |
| AGTTGTTGGGCCAGAAGAAA+TGG | - | Chr3:29690815-29690834 | MsG0380013061.01.T01:CDS | 45.0% | |
| ATCACCATGTTTGGAGACTC+TGG | - | Chr3:29690152-29690171 | MsG0380013061.01.T01:intron | 45.0% | |
| GTTGTTGGGCCAGAAGAAAT+GGG | - | Chr3:29690816-29690835 | MsG0380013061.01.T01:CDS | 45.0% | |
| TTCTTCTGGCCCAACAACTT+AGG | + | Chr3:29690814-29690833 | None:intergenic | 45.0% | |
| ! | GCAACCGGCGAATTTTTGTT+TGG | - | Chr3:29690117-29690136 | MsG0380013061.01.T01:intron | 45.0% |
| ! | TGAGCATAGCTCAGTTGGTA+AGG | - | Chr3:29690326-29690345 | MsG0380013061.01.T01:intron | 45.0% |
| !!! | TGTGTTTTTTTGTGGTGGCC+GGG | + | Chr3:29690397-29690416 | None:intergenic | 45.0% |
| CTGGATCTCGACTTTGCAAC+CGG | - | Chr3:29690102-29690121 | MsG0380013061.01.T01:intron | 50.0% | |
| !!! | GTGTTTTTTTGTGGTGGCCG+GGG | + | Chr3:29690396-29690415 | None:intergenic | 50.0% |
| CCAACTGAGCTATGCTCACG+AGG | + | Chr3:29690324-29690343 | None:intergenic | 55.0% | |
| CCTCGTGAGCATAGCTCAGT+TGG | - | Chr3:29690321-29690340 | MsG0380013061.01.T01:intron | 55.0% | |
| AAGGTCCGGGGTTCAAACCC+CGG | - | Chr3:29690376-29690395 | MsG0380013061.01.T01:intron | 60.0% | |
| GGTGGCCGGGGTTTGAACCC+CGG | + | Chr3:29690384-29690403 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 29689700 | 29690888 | 29689700 | ID=MsG0380013061.01;Name=MsG0380013061.01 |
| Chr3 | mRNA | 29689700 | 29690888 | 29689700 | ID=MsG0380013061.01.T01;Parent=MsG0380013061.01;Name=MsG0380013061.01.T01;_AED=0.45;_eAED=0.47;_QI=0|0.5|0.6|0.8|0.5|0.6|5|0|184 |
| Chr3 | exon | 29690790 | 29690888 | 29690790 | ID=MsG0380013061.01.T01:exon:6921;Parent=MsG0380013061.01.T01 |
| Chr3 | exon | 29690406 | 29690546 | 29690406 | ID=MsG0380013061.01.T01:exon:6922;Parent=MsG0380013061.01.T01 |
| Chr3 | exon | 29690246 | 29690308 | 29690246 | ID=MsG0380013061.01.T01:exon:6923;Parent=MsG0380013061.01.T01 |
| Chr3 | exon | 29689700 | 29689951 | 29689700 | ID=MsG0380013061.01.T01:exon:6924;Parent=MsG0380013061.01.T01 |
| Chr3 | CDS | 29690790 | 29690888 | 29690790 | ID=MsG0380013061.01.T01:cds;Parent=MsG0380013061.01.T01 |
| Chr3 | CDS | 29690406 | 29690546 | 29690406 | ID=MsG0380013061.01.T01:cds;Parent=MsG0380013061.01.T01 |
| Chr3 | CDS | 29690246 | 29690308 | 29690246 | ID=MsG0380013061.01.T01:cds;Parent=MsG0380013061.01.T01 |
| Chr3 | CDS | 29689700 | 29689951 | 29689700 | ID=MsG0380013061.01.T01:cds;Parent=MsG0380013061.01.T01 |
| Gene Sequence |
| Protein sequence |
