Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042454.01.T01 | RHN71862.1 | 91.971 | 137 | 11 | 0 | 13 | 149 | 1 | 137 | 2.67E-69 | 215 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042454.01.T01 | A0A396J3D3 | 91.971 | 137 | 11 | 0 | 13 | 149 | 1 | 137 | 1.28e-69 | 215 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048515.01 | MsG0880042454.01 | 0.814423 | 1.552304e-51 | 1.041210e-48 |
| MsG0180000470.01 | MsG0880042454.01 | 0.811490 | 6.820960e-51 | 4.228151e-48 |
| MsG0180001410.01 | MsG0880042454.01 | 0.800313 | 1.527931e-48 | 7.092328e-46 |
| MsG0180002089.01 | MsG0880042454.01 | 0.823065 | 1.692942e-53 | 1.442476e-50 |
| MsG0180002233.01 | MsG0880042454.01 | 0.830314 | 3.149782e-55 | 3.306496e-52 |
| MsG0180002234.01 | MsG0880042454.01 | 0.854457 | 1.215438e-61 | 2.715980e-58 |
| MsG0180002282.01 | MsG0880042454.01 | 0.808965 | 2.390167e-50 | 1.385653e-47 |
| MsG0180002563.01 | MsG0880042454.01 | 0.821496 | 3.913920e-53 | 3.189337e-50 |
| MsG0180002667.01 | MsG0880042454.01 | 0.833588 | 4.891945e-56 | 5.658976e-53 |
| MsG0280006325.01 | MsG0880042454.01 | 0.804095 | 2.546225e-49 | 1.301344e-46 |
| MsG0780040576.01 | MsG0880042454.01 | -0.826496 | 2.627081e-54 | 2.467718e-51 |
| MsG0780040596.01 | MsG0880042454.01 | 0.823541 | 1.310102e-53 | 1.131296e-50 |
| MsG0780041419.01 | MsG0880042454.01 | 0.819611 | 1.060587e-52 | 8.200616e-50 |
| MsG0880041916.01 | MsG0880042454.01 | 0.800263 | 1.564352e-48 | 7.251914e-46 |
| MsG0880042161.01 | MsG0880042454.01 | 0.817133 | 3.861562e-52 | 2.788831e-49 |
| MsG0880042196.01 | MsG0880042454.01 | 0.820791 | 5.689471e-53 | 4.546144e-50 |
| MsG0880042247.01 | MsG0880042454.01 | 0.828582 | 8.299371e-55 | 8.283841e-52 |
| MsG0880042454.01 | MsG0880042886.01 | 0.835475 | 1.641766e-56 | 2.009849e-53 |
| MsG0880042454.01 | MsG0880043941.01 | 0.805508 | 1.290277e-49 | 6.838360e-47 |
| MsG0880042454.01 | MsG0880044323.01 | 0.822631 | 2.136249e-53 | 1.798078e-50 |
| MsG0880042454.01 | MsG0880044573.01 | 0.803022 | 4.251241e-49 | 2.113527e-46 |
| MsG0880042454.01 | MsG0880045691.01 | 0.811898 | 5.561076e-51 | 3.484379e-48 |
| MsG0880042454.01 | MsG0880045992.01 | 0.804083 | 2.560617e-49 | 1.308305e-46 |
| MsG0280011415.01 | MsG0880042454.01 | 0.810115 | 1.353411e-50 | 8.089508e-48 |
| MsG0380011759.01 | MsG0880042454.01 | 0.843917 | 1.043301e-58 | 1.657195e-55 |
| MsG0380012007.01 | MsG0880042454.01 | 0.808291 | 3.328822e-50 | 1.896045e-47 |
| MsG0380012009.01 | MsG0880042454.01 | 0.808173 | 3.527517e-50 | 2.003064e-47 |
| MsG0380013061.01 | MsG0880042454.01 | 0.810269 | 1.253509e-50 | 7.522386e-48 |
| MsG0380013896.01 | MsG0880042454.01 | 0.816624 | 5.024048e-52 | 3.578093e-49 |
| MsG0380014867.01 | MsG0880042454.01 | 0.807634 | 4.592903e-50 | 2.571199e-47 |
| MsG0680032157.01 | MsG0880042454.01 | 0.813050 | 3.114547e-51 | 2.012526e-48 |
| MsG0680032158.01 | MsG0880042454.01 | 0.856043 | 4.200170e-62 | 9.893871e-59 |
| MsG0680032801.01 | MsG0880042454.01 | 0.835591 | 1.534786e-56 | 1.885370e-53 |
| MsG0680032844.01 | MsG0880042454.01 | 0.831264 | 1.842672e-55 | 1.989147e-52 |
| MsG0680033379.01 | MsG0880042454.01 | 0.827617 | 1.417156e-54 | 1.375083e-51 |
| MsG0680034565.01 | MsG0880042454.01 | 0.809624 | 1.726208e-50 | 1.018597e-47 |
| MsG0480019435.01 | MsG0880042454.01 | 0.800181 | 1.625472e-48 | 7.519359e-46 |
| MsG0480019730.01 | MsG0880042454.01 | 0.849967 | 2.301099e-60 | 4.438578e-57 |
| MsG0480021869.01 | MsG0880042454.01 | 0.825014 | 5.905053e-54 | 5.318215e-51 |
| MsG0480022259.01 | MsG0880042454.01 | 0.802022 | 6.832273e-49 | 3.311465e-46 |
| MsG0480022947.01 | MsG0880042454.01 | 0.815896 | 7.308486e-52 | 5.102130e-49 |
| MsG0480022951.01 | MsG0880042454.01 | 0.804731 | 1.876264e-49 | 9.748367e-47 |
| MsG0380015720.01 | MsG0880042454.01 | 0.807947 | 3.940689e-50 | 2.224196e-47 |
| MsG0380017326.01 | MsG0880042454.01 | 0.813358 | 2.665733e-51 | 1.737090e-48 |
| MsG0380017822.01 | MsG0880042454.01 | 0.837157 | 6.133439e-57 | 7.898573e-54 |
| MsG0380017848.01 | MsG0880042454.01 | 0.811372 | 7.236368e-51 | 4.471500e-48 |
| MsG0480018318.01 | MsG0880042454.01 | 0.819521 | 1.111595e-52 | 8.574131e-50 |
| MsG0580026197.01 | MsG0880042454.01 | 0.830710 | 2.519813e-55 | 2.676133e-52 |
| MsG0580027018.01 | MsG0880042454.01 | 0.805348 | 1.393833e-49 | 7.357138e-47 |
| MsG0580027142.01 | MsG0880042454.01 | 0.829341 | 5.434806e-55 | 5.547419e-52 |
| MsG0580027687.01 | MsG0880042454.01 | 0.814316 | 1.639313e-51 | 1.096383e-48 |
| MsG0580027688.01 | MsG0880042454.01 | 0.836893 | 7.165183e-57 | 9.154427e-54 |
| MsG0580027922.01 | MsG0880042454.01 | 0.822390 | 2.430146e-53 | 2.031404e-50 |
| MsG0580029204.01 | MsG0880042454.01 | 0.839334 | 1.686506e-57 | 2.321651e-54 |
| MsG0580029500.01 | MsG0880042454.01 | -0.800589 | 1.342856e-48 | 6.276676e-46 |
| MsG0580030050.01 | MsG0880042454.01 | 0.835631 | 1.499783e-56 | 1.844517e-53 |
| MsG0280006588.01 | MsG0880042454.01 | 0.818486 | 1.911900e-52 | 1.433047e-49 |
| MsG0280006626.01 | MsG0880042454.01 | 0.803254 | 3.806351e-49 | 1.903744e-46 |
| MsG0280008759.01 | MsG0880042454.01 | 0.822924 | 1.825507e-53 | 1.549231e-50 |
| MsG0280009646.01 | MsG0880042454.01 | 0.802213 | 6.240805e-49 | 3.039537e-46 |
| MsG0280010117.01 | MsG0880042454.01 | 0.807197 | 5.686326e-50 | 3.147397e-47 |
| MsG0280010510.01 | MsG0880042454.01 | 0.812141 | 4.922332e-51 | 3.104799e-48 |
| MsG0680035828.01 | MsG0880042454.01 | 0.827311 | 1.677718e-54 | 1.613790e-51 |
| MsG0780036080.01 | MsG0880042454.01 | 0.801202 | 1.006481e-48 | 4.778048e-46 |
| MsG0780037457.01 | MsG0880042454.01 | 0.806219 | 9.147343e-50 | 4.937493e-47 |
| MsG0780038439.01 | MsG0880042454.01 | 0.828355 | 9.416694e-55 | 9.336905e-52 |
| MsG0780039178.01 | MsG0880042454.01 | 0.848566 | 5.647312e-60 | 1.040687e-56 |
| MsG0780039282.01 | MsG0880042454.01 | 0.812256 | 4.646698e-51 | 2.940082e-48 |
| MsG0780039423.01 | MsG0880042454.01 | 0.801159 | 1.026738e-48 | 4.869121e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042454.01.T01 | MTR_2g012543 | 91.971 | 137 | 11 | 0 | 13 | 149 | 1 | 137 | 4.44e-73 | 215 |
| MsG0880042454.01.T01 | MTR_6g026970 | 74.101 | 139 | 31 | 1 | 1 | 134 | 1 | 139 | 2.70e-60 | 185 |
| MsG0880042454.01.T01 | MTR_3g052068 | 49.635 | 137 | 67 | 1 | 13 | 149 | 1 | 135 | 2.09e-40 | 132 |
| MsG0880042454.01.T01 | MTR_5g056670 | 48.175 | 137 | 69 | 1 | 13 | 149 | 1 | 135 | 5.57e-40 | 131 |
| MsG0880042454.01.T01 | MTR_4g056340 | 48.175 | 137 | 69 | 1 | 13 | 149 | 1 | 135 | 5.57e-40 | 131 |
| MsG0880042454.01.T01 | MTR_8g466420 | 52.000 | 125 | 58 | 1 | 25 | 149 | 9 | 131 | 1.25e-39 | 130 |
| MsG0880042454.01.T01 | MTR_2g066040 | 48.344 | 151 | 69 | 3 | 6 | 149 | 4 | 152 | 3.77e-39 | 130 |
| MsG0880042454.01.T01 | MTR_6g024220 | 48.175 | 137 | 69 | 1 | 13 | 149 | 1 | 135 | 9.19e-39 | 128 |
| MsG0880042454.01.T01 | MTR_2g094990 | 46.715 | 137 | 71 | 1 | 13 | 149 | 1 | 135 | 2.03e-37 | 125 |
| MsG0880042454.01.T01 | MTR_6g071415 | 51.538 | 130 | 59 | 2 | 6 | 135 | 4 | 129 | 6.79e-36 | 120 |
| MsG0880042454.01.T01 | MTR_7g018900 | 47.222 | 144 | 74 | 1 | 6 | 149 | 4 | 145 | 1.22e-33 | 115 |
| MsG0880042454.01.T01 | MTR_5g047980 | 47.586 | 145 | 67 | 2 | 5 | 149 | 38 | 173 | 5.48e-33 | 115 |
| MsG0880042454.01.T01 | MTR_6g059700 | 72.973 | 74 | 17 | 2 | 1 | 74 | 1 | 71 | 4.62e-32 | 109 |
| MsG0880042454.01.T01 | MTR_5g076090 | 51.562 | 128 | 56 | 1 | 28 | 149 | 25 | 152 | 1.28e-30 | 108 |
| MsG0880042454.01.T01 | MTR_8g103580 | 46.763 | 139 | 59 | 3 | 11 | 149 | 23 | 146 | 1.90e-30 | 107 |
| MsG0880042454.01.T01 | MTR_2g461780 | 48.214 | 112 | 56 | 1 | 38 | 149 | 28 | 137 | 1.94e-30 | 107 |
| MsG0880042454.01.T01 | MTR_6g045260 | 47.368 | 152 | 71 | 3 | 6 | 149 | 2 | 152 | 2.27e-30 | 107 |
| MsG0880042454.01.T01 | MTR_5g049800 | 46.753 | 154 | 69 | 4 | 6 | 149 | 2 | 152 | 4.17e-30 | 107 |
| MsG0880042454.01.T01 | MTR_7g055780 | 44.037 | 109 | 55 | 1 | 35 | 137 | 2 | 110 | 6.34e-30 | 105 |
| MsG0880042454.01.T01 | MTR_2g047865 | 45.968 | 124 | 57 | 2 | 35 | 149 | 5 | 127 | 4.74e-29 | 103 |
| MsG0880042454.01.T01 | MTR_1g024970 | 51.818 | 110 | 45 | 2 | 25 | 127 | 29 | 137 | 3.03e-28 | 102 |
| MsG0880042454.01.T01 | MTR_4g028240 | 47.619 | 126 | 64 | 1 | 5 | 130 | 3 | 126 | 3.73e-28 | 101 |
| MsG0880042454.01.T01 | MTR_6g079080 | 44.144 | 111 | 54 | 2 | 34 | 136 | 35 | 145 | 4.53e-27 | 99.0 |
| MsG0880042454.01.T01 | MTR_2g048830 | 60.294 | 68 | 26 | 1 | 28 | 95 | 25 | 91 | 2.51e-22 | 85.1 |
| MsG0880042454.01.T01 | MTR_1g055150 | 46.218 | 119 | 56 | 2 | 25 | 136 | 29 | 146 | 6.97e-21 | 83.2 |
| MsG0880042454.01.T01 | MTR_3g467190 | 48.611 | 72 | 36 | 1 | 34 | 104 | 35 | 106 | 2.33e-18 | 75.5 |
| MsG0880042454.01.T01 | MTR_8g021280 | 67.347 | 49 | 16 | 0 | 28 | 76 | 25 | 73 | 2.01e-17 | 72.8 |
| MsG0880042454.01.T01 | MTR_2g099650 | 42.308 | 104 | 57 | 1 | 49 | 149 | 2 | 105 | 4.15e-17 | 72.4 |
| MsG0880042454.01.T01 | MTR_5g074720 | 47.561 | 82 | 40 | 1 | 30 | 108 | 29 | 110 | 3.90e-16 | 70.5 |
| MsG0880042454.01.T01 | MTR_7g077640 | 40.000 | 110 | 50 | 3 | 25 | 118 | 5 | 114 | 7.45e-14 | 64.3 |
| MsG0880042454.01.T01 | MTR_8g468340 | 48.193 | 83 | 34 | 2 | 67 | 149 | 2 | 75 | 9.15e-13 | 60.1 |
| MsG0880042454.01.T01 | MTR_8g468130 | 42.222 | 90 | 42 | 3 | 44 | 123 | 12 | 101 | 1.32e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGTAACCAATCCGAATTT+TGG | 0.206838 | 8:-9085475 | MsG0880042454.01.T01:CDS |
| AAGGTTGCAACTACTTTAAT+TGG | 0.258049 | 8:-9085408 | MsG0880042454.01.T01:CDS |
| AGTTGCAACCTTTATCAAAT+TGG | 0.267358 | 8:+9085419 | None:intergenic |
| TTGTAACAATAACAATCTTT+TGG | 0.267393 | 8:-9085212 | MsG0880042454.01.T01:CDS |
| TACAAGAACCAATTTGATAA+AGG | 0.312390 | 8:-9085427 | MsG0880042454.01.T01:CDS |
| GCATTTGGAATTGTAAGCTT+TGG | 0.321533 | 8:-9085265 | MsG0880042454.01.T01:CDS |
| TTGAAAATATCTATTGCATT+TGG | 0.335251 | 8:-9085280 | MsG0880042454.01.T01:CDS |
| CATTTGGAATTGTAAGCTTT+GGG | 0.340209 | 8:-9085264 | MsG0880042454.01.T01:CDS |
| AACTCGTCAGCTTCGTGTTA+TGG | 0.364898 | 8:-9085568 | MsG0880042454.01.T01:CDS |
| CGTGTTATGGATCTATTCTC+AGG | 0.442786 | 8:-9085555 | MsG0880042454.01.T01:CDS |
| CTTTAATTGGTTTGATGCAA+GGG | 0.456776 | 8:-9085395 | MsG0880042454.01.T01:CDS |
| TCCACTTTGCTGCATAGAGT+TGG | 0.493116 | 8:+9085591 | None:intergenic |
| ACAATATTGTTAACAATACT+AGG | 0.494077 | 8:+9085236 | None:intergenic |
| ACTTTAATTGGTTTGATGCA+AGG | 0.501593 | 8:-9085396 | MsG0880042454.01.T01:CDS |
| ACCAACTCTATGCAGCAAAG+TGG | 0.591206 | 8:-9085592 | MsG0880042454.01.T01:CDS |
| TTTCGAATTCTGGGAAACAG+TGG | 0.608184 | 8:+9085615 | None:intergenic |
| TCATGTAGAGACTGTAGATG+TGG | 0.685482 | 8:-9085526 | MsG0880042454.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATATTGTTAACAATACT+AGG | + | Chr8:9085578-9085597 | None:intergenic | 20.0% |
| !! | TTGAAAATATCTATTGCATT+TGG | - | Chr8:9085531-9085550 | MsG0880042454.01.T01:CDS | 20.0% |
| !!! | AATTTTGGGAAAAAAATTTG+GGG | - | Chr8:9085351-9085370 | MsG0880042454.01.T01:CDS | 20.0% |
| !!! | CAAATTTTTTTCCCAAAATT+CGG | + | Chr8:9085351-9085370 | None:intergenic | 20.0% |
| !!! | GAATTTTGGGAAAAAAATTT+GGG | - | Chr8:9085350-9085369 | MsG0880042454.01.T01:CDS | 20.0% |
| !!! | TGTAACAATAACAATCTTTT+GGG | - | Chr8:9085600-9085619 | MsG0880042454.01.T01:CDS | 20.0% |
| !!! | TTGTAACAATAACAATCTTT+TGG | - | Chr8:9085599-9085618 | MsG0880042454.01.T01:CDS | 20.0% |
| ! | TACAAGAACCAATTTGATAA+AGG | - | Chr8:9085384-9085403 | MsG0880042454.01.T01:CDS | 25.0% |
| !!! | CGAATTTTGGGAAAAAAATT+TGG | - | Chr8:9085349-9085368 | MsG0880042454.01.T01:CDS | 25.0% |
| AAGGTTGCAACTACTTTAAT+TGG | - | Chr8:9085403-9085422 | MsG0880042454.01.T01:CDS | 30.0% | |
| AGTTGCAACCTTTATCAAAT+TGG | + | Chr8:9085395-9085414 | None:intergenic | 30.0% | |
| ! | ATGTAACCAATCCGAATTTT+GGG | - | Chr8:9085337-9085356 | MsG0880042454.01.T01:CDS | 30.0% |
| ! | CATTTGGAATTGTAAGCTTT+GGG | - | Chr8:9085547-9085566 | MsG0880042454.01.T01:CDS | 30.0% |
| !! | ACTTTAATTGGTTTGATGCA+AGG | - | Chr8:9085415-9085434 | MsG0880042454.01.T01:CDS | 30.0% |
| !! | CTTTAATTGGTTTGATGCAA+GGG | - | Chr8:9085416-9085435 | MsG0880042454.01.T01:CDS | 30.0% |
| !!! | TTTTTTCCCAAAATTCGGAT+TGG | + | Chr8:9085346-9085365 | None:intergenic | 30.0% |
| CATTAGCTCGTTCACAAAAA+TGG | - | Chr8:9085508-9085527 | MsG0880042454.01.T01:CDS | 35.0% | |
| ! | GATGTAACCAATCCGAATTT+TGG | - | Chr8:9085336-9085355 | MsG0880042454.01.T01:CDS | 35.0% |
| ! | GCATTTGGAATTGTAAGCTT+TGG | - | Chr8:9085546-9085565 | MsG0880042454.01.T01:CDS | 35.0% |
| !!! | AGAGTTGGTTTTCGAATTCT+GGG | + | Chr8:9085208-9085227 | None:intergenic | 35.0% |
| !!! | TAGAGTTGGTTTTCGAATTC+TGG | + | Chr8:9085209-9085228 | None:intergenic | 35.0% |
| CGTGTTATGGATCTATTCTC+AGG | - | Chr8:9085256-9085275 | MsG0880042454.01.T01:CDS | 40.0% | |
| TCATGTAGAGACTGTAGATG+TGG | - | Chr8:9085285-9085304 | MsG0880042454.01.T01:CDS | 40.0% | |
| ! | TTTCGAATTCTGGGAAACAG+TGG | + | Chr8:9085199-9085218 | None:intergenic | 40.0% |
| AACTCGTCAGCTTCGTGTTA+TGG | - | Chr8:9085243-9085262 | MsG0880042454.01.T01:CDS | 45.0% | |
| ACCAACTCTATGCAGCAAAG+TGG | - | Chr8:9085219-9085238 | MsG0880042454.01.T01:CDS | 45.0% | |
| TCCACTTTGCTGCATAGAGT+TGG | + | Chr8:9085223-9085242 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 9085192 | 9085641 | 9085192 | ID=MsG0880042454.01;Name=MsG0880042454.01 |
| Chr8 | mRNA | 9085192 | 9085641 | 9085192 | ID=MsG0880042454.01.T01;Parent=MsG0880042454.01;Name=MsG0880042454.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|149 |
| Chr8 | exon | 9085192 | 9085641 | 9085192 | ID=MsG0880042454.01.T01:exon:2372;Parent=MsG0880042454.01.T01 |
| Chr8 | CDS | 9085192 | 9085641 | 9085192 | ID=MsG0880042454.01.T01:cds;Parent=MsG0880042454.01.T01 |
| Gene Sequence |
| Protein sequence |
