AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014045.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014045.01.T01 MTR_4g051130 88.889 306 6 1 1 278 283 588 0.0 543
MsG0380014045.01.T01 MTR_0002s0510 71.290 310 52 3 1 273 348 657 5.36e-138 430
MsG0380014045.01.T01 MTR_4g055180 94.915 59 3 0 79 137 23 81 9.09e-35 122
MsG0380014045.01.T01 MTR_2g040120 66.667 69 23 0 89 157 9 77 1.85e-24 94.4
MsG0380014045.01.T01 MTR_0552s0020 100.000 42 0 0 225 266 1 42 2.47e-22 88.6
MsG0380014045.01.T01 MTR_4g021033 100.000 42 0 0 225 266 1 42 2.47e-22 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014045.01.T01 ATCG00190 83.224 304 21 1 1 274 296 599 6.96e-177 516

Find 67 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGAGAAAATTGAGTTATT+TGG 0.135693 3:+48723132 MsG0380014045.01.T01:CDS
CATTTGATTGGAATGAAATT+TGG 0.153768 3:+48722839 None:intergenic
TACTGACAAGATCATTTATT+TGG 0.156584 3:+48723739 MsG0380014045.01.T01:CDS
GGAATCAATGTAGGACTTAT+TGG 0.263649 3:+48723250 MsG0380014045.01.T01:CDS
AGCACTGAATCGAGGTATTC+AGG 0.270037 3:+48723421 MsG0380014045.01.T01:CDS
GATCTATTACAAGATCAATT+TGG 0.270369 3:+48722920 MsG0380014045.01.T01:CDS
GATTCATAAGTGGTTGTTAA+TGG 0.270524 3:-48723043 None:intergenic
CAAGATCATTTATTTGGAAA+TGG 0.278779 3:+48723745 MsG0380014045.01.T01:CDS
CGATCTAAAACTTGAGATAA+TGG 0.281648 3:-48723082 None:intergenic
ATAATTCGAGGAACTATATC+TGG 0.305120 3:+48722968 MsG0380014045.01.T01:CDS
GCATCATTATGCTCGATAAA+AGG 0.305503 3:-48723560 None:intergenic
GGGAGAAAATTGAGTTATTT+GGG 0.307341 3:+48723133 MsG0380014045.01.T01:CDS
CGCCAAGTGGCCTTAGATTC+AGG 0.326928 3:+48723671 MsG0380014045.01.T01:CDS
AACGATCTTTCCTGCGTGTT+CGG 0.330632 3:-48723708 None:intergenic
TCACGCAAGGATTGGTCCTT+GGG 0.341088 3:+48723285 MsG0380014045.01.T01:CDS
GTCAAGCAGTTCCTCTTTCT+CGG 0.344262 3:+48723615 MsG0380014045.01.T01:CDS
CCATGAACTATTTGGGTCAA+TGG 0.354074 3:-48723112 None:intergenic
CGGTCCGAAAAGTGCATTGT+TGG 0.359288 3:+48723635 MsG0380014045.01.T01:CDS
AGCGGGAACTCCTGAATCTA+AGG 0.364603 3:-48723681 None:intergenic
TATGCTCGATAAAAGGAATC+AGG 0.367331 3:-48723553 None:intergenic
TTGGGTCCTGGAGGATTGAC+GGG 0.382208 3:+48723151 MsG0380014045.01.T01:CDS
AAGATCATTTATTTGGAAAT+GGG 0.382872 3:+48723746 MsG0380014045.01.T01:CDS
ATTCCTTCCGAGGTGTCAAT+TGG 0.403361 3:-48723232 None:intergenic
TGTAGGACTTATTGGATCCT+TGG 0.411030 3:+48723258 MsG0380014045.01.T01:CDS
TCTAACTATTGCATGGGAAC+AGG 0.414074 3:+48723481 MsG0380014045.01.T01:CDS
GAAAAGTGCATTGTTGGAAC+TGG 0.419529 3:+48723641 MsG0380014045.01.T01:CDS
TTCACGCAAGGATTGGTCCT+TGG 0.420745 3:+48723284 MsG0380014045.01.T01:CDS
TTTGGGTCCTGGAGGATTGA+CGG 0.423824 3:+48723150 MsG0380014045.01.T01:CDS
AACTATTTGGGTCAATGGAT+TGG 0.429624 3:-48723107 None:intergenic
CCATTGACCCAAATAGTTCA+TGG 0.431227 3:+48723112 MsG0380014045.01.T01:CDS
GATATCCATCCTAGTCACTA+TGG 0.449002 3:+48723199 MsG0380014045.01.T01:CDS
AAATTGAGTTATTTGGGTCC+TGG 0.453699 3:+48723139 MsG0380014045.01.T01:CDS
GATCTAAAACTTGAGATAAT+GGG 0.468332 3:-48723081 None:intergenic
ATTACAAGATCAATTTGGAT+TGG 0.471878 3:+48722925 MsG0380014045.01.T01:CDS
AAGAATTTCTAACTATTGCA+TGG 0.481440 3:+48723474 MsG0380014045.01.T01:CDS
CCTGCGTGTTCGGCTATAGC+GGG 0.483049 3:-48723698 None:intergenic
CGTTTAGAAAATATAATTCG+AGG 0.485783 3:+48722956 MsG0380014045.01.T01:CDS
ATTGGAATGAAATTTGGAAT+GGG 0.491267 3:+48722845 None:intergenic
TTGGCAATTCACGCAAGGAT+TGG 0.498707 3:+48723277 MsG0380014045.01.T01:CDS
GATTGGAATGAAATTTGGAA+TGG 0.498930 3:+48722844 None:intergenic
GTGCATTGTTGGAACTGGAT+TGG 0.504759 3:+48723646 MsG0380014045.01.T01:CDS
CTCCTGAATCTAAGGCCACT+TGG 0.506387 3:-48723673 None:intergenic
GTTCGCCCCGTCAATCCTCC+AGG 0.515843 3:-48723157 None:intergenic
AATTCTTGACGGTATCGAGC+TGG 0.536702 3:-48723458 None:intergenic
CAATAGTTAGAAATTCTTGA+CGG 0.539101 3:-48723469 None:intergenic
TCCTGCGTGTTCGGCTATAG+CGG 0.549505 3:-48723699 None:intergenic
AGAATTTCTAACTATTGCAT+GGG 0.549733 3:+48723475 MsG0380014045.01.T01:CDS
CATTGACCCAAATAGTTCAT+GGG 0.556065 3:+48723113 MsG0380014045.01.T01:CDS
ATCTTGTAATAGATCTGCTA+CGG 0.559735 3:-48722912 None:intergenic
GTCACTAAATTCTGAGGAGT+AGG 0.563396 3:-48723013 None:intergenic
TCCTACATTGATTCCTTCCG+AGG 0.572209 3:-48723242 None:intergenic
CAAATGCGTCCATAGTGACT+AGG 0.573493 3:-48723208 None:intergenic
TGTCCAATTGACACCTCGGA+AGG 0.584582 3:+48723229 MsG0380014045.01.T01:CDS
ATGCTCGATAAAAGGAATCA+GGG 0.587469 3:-48723552 None:intergenic
CACGCAAGGATTGGTCCTTG+GGG 0.608804 3:+48723286 MsG0380014045.01.T01:CDS
GATCCTTGGCAATTCACGCA+AGG 0.614349 3:+48723272 MsG0380014045.01.T01:CDS
AACTGGATTGGAACGCCAAG+TGG 0.615266 3:+48723658 MsG0380014045.01.T01:CDS
GTTGAAGTCACTAAATTCTG+AGG 0.617323 3:-48723019 None:intergenic
AATCCTTGCGTGAATTGCCA+AGG 0.621341 3:-48723275 None:intergenic
CATTTGTCCAATTGACACCT+CGG 0.622149 3:+48723225 MsG0380014045.01.T01:CDS
TGCGTCCATAGTGACTAGGA+TGG 0.626242 3:-48723204 None:intergenic
ACGCAAGGATTGGTCCTTGG+GGG 0.629421 3:+48723287 MsG0380014045.01.T01:CDS
TTGAGTTATTTGGGTCCTGG+AGG 0.630319 3:+48723142 MsG0380014045.01.T01:CDS
TGGGTCCTGGAGGATTGACG+GGG 0.631061 3:+48723152 MsG0380014045.01.T01:CDS
ACCTCGGAAGGAATCAATGT+AGG 0.642821 3:+48723241 MsG0380014045.01.T01:CDS
CCCGCTATAGCCGAACACGC+AGG 0.684746 3:+48723698 MsG0380014045.01.T01:CDS
ACTGAATCGAGGTATTCAGG+AGG 0.734798 3:+48723424 MsG0380014045.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATCATTTATTTGGAAAT+GGG + Chr3:48723746-48723765 MsG0380014045.01.T01:CDS 20.0%
!! TCTCAGAAATTTCAAAAAAA+CGG - Chr3:48723325-48723344 None:intergenic 20.0%
! AAGAATTTCTAACTATTGCA+TGG + Chr3:48723474-48723493 MsG0380014045.01.T01:CDS 25.0%
! AAGCTCCAATAGAAAAATAT+TGG - Chr3:48723532-48723551 None:intergenic 25.0%
! AAGTAGAGATGAATACTATA+TGG + Chr3:48723379-48723398 MsG0380014045.01.T01:intron 25.0%
! AGAATTTCTAACTATTGCAT+GGG + Chr3:48723475-48723494 MsG0380014045.01.T01:CDS 25.0%
! ATATAGTATTCATCTCTACT+TGG - Chr3:48723380-48723399 None:intergenic 25.0%
! ATTACAAGATCAATTTGGAT+TGG + Chr3:48722925-48722944 MsG0380014045.01.T01:CDS 25.0%
! CAAGATCATTTATTTGGAAA+TGG + Chr3:48723745-48723764 MsG0380014045.01.T01:CDS 25.0%
! CAATAGTTAGAAATTCTTGA+CGG - Chr3:48723472-48723491 None:intergenic 25.0%
! CCAATAGAAAAATATTGGAA+GGG - Chr3:48723527-48723546 None:intergenic 25.0%
! CGTTTAGAAAATATAATTCG+AGG + Chr3:48722956-48722975 MsG0380014045.01.T01:CDS 25.0%
! GATCTATTACAAGATCAATT+TGG + Chr3:48722920-48722939 MsG0380014045.01.T01:CDS 25.0%
! TACTGACAAGATCATTTATT+TGG + Chr3:48723739-48723758 MsG0380014045.01.T01:CDS 25.0%
! TCCAATAGAAAAATATTGGA+AGG - Chr3:48723528-48723547 None:intergenic 25.0%
!! GATCTAAAACTTGAGATAAT+GGG - Chr3:48723084-48723103 None:intergenic 25.0%
ATAATTCGAGGAACTATATC+TGG + Chr3:48722968-48722987 MsG0380014045.01.T01:CDS 30.0%
ATCTTGTAATAGATCTGCTA+CGG - Chr3:48722915-48722934 None:intergenic 30.0%
GATTCATAAGTGGTTGTTAA+TGG - Chr3:48723046-48723065 None:intergenic 30.0%
GGGAGAAAATTGAGTTATTT+GGG + Chr3:48723133-48723152 MsG0380014045.01.T01:CDS 30.0%
TCCAAAAAACGATTCATAAG+TGG - Chr3:48723056-48723075 None:intergenic 30.0%
TGGGAGAAAATTGAGTTATT+TGG + Chr3:48723132-48723151 MsG0380014045.01.T01:CDS 30.0%
! ATTTTCTCCCATGAACTATT+TGG - Chr3:48723123-48723142 None:intergenic 30.0%
! CCCTTCCAATATTTTTCTAT+TGG + Chr3:48723524-48723543 MsG0380014045.01.T01:CDS 30.0%
! CGATCTAAAACTTGAGATAA+TGG - Chr3:48723085-48723104 None:intergenic 30.0%
! TTTTCTCCCATGAACTATTT+GGG - Chr3:48723122-48723141 None:intergenic 30.0%
!!! AATTTTTTAGCACTGAATCG+AGG + Chr3:48723413-48723432 MsG0380014045.01.T01:CDS 30.0%
!!! ACCACTTATGAATCGTTTTT+TGG + Chr3:48723052-48723071 MsG0380014045.01.T01:CDS 30.0%
AAATTGAGTTATTTGGGTCC+TGG + Chr3:48723139-48723158 MsG0380014045.01.T01:CDS 35.0%
AACTATTTGGGTCAATGGAT+TGG - Chr3:48723110-48723129 None:intergenic 35.0%
ATGCTCGATAAAAGGAATCA+GGG - Chr3:48723555-48723574 None:intergenic 35.0%
CATTGACCCAAATAGTTCAT+GGG + Chr3:48723113-48723132 MsG0380014045.01.T01:CDS 35.0%
GCATCATTATGCTCGATAAA+AGG - Chr3:48723563-48723582 None:intergenic 35.0%
GGAATCAATGTAGGACTTAT+TGG + Chr3:48723250-48723269 MsG0380014045.01.T01:CDS 35.0%
GTTGAAGTCACTAAATTCTG+AGG - Chr3:48723022-48723041 None:intergenic 35.0%
TATGCTCGATAAAAGGAATC+AGG - Chr3:48723556-48723575 None:intergenic 35.0%
! AGTTCCAACAATGCACTTTT+CGG - Chr3:48723642-48723661 None:intergenic 35.0%
! TGAATACTATATGGTAGCGA+CGG + Chr3:48723388-48723407 MsG0380014045.01.T01:intron 35.0%
CATTTGTCCAATTGACACCT+CGG + Chr3:48723225-48723244 MsG0380014045.01.T01:CDS 40.0%
CCATGAACTATTTGGGTCAA+TGG - Chr3:48723115-48723134 None:intergenic 40.0%
CCATTGACCCAAATAGTTCA+TGG + Chr3:48723112-48723131 MsG0380014045.01.T01:CDS 40.0%
GATATCCATCCTAGTCACTA+TGG + Chr3:48723199-48723218 MsG0380014045.01.T01:CDS 40.0%
TCTAACTATTGCATGGGAAC+AGG + Chr3:48723481-48723500 MsG0380014045.01.T01:CDS 40.0%
TGTAGGACTTATTGGATCCT+TGG + Chr3:48723258-48723277 MsG0380014045.01.T01:CDS 40.0%
! GAAAAGTGCATTGTTGGAAC+TGG + Chr3:48723641-48723660 MsG0380014045.01.T01:CDS 40.0%
! GAATACTATATGGTAGCGAC+GGG + Chr3:48723389-48723408 MsG0380014045.01.T01:intron 40.0%
! GTCACTAAATTCTGAGGAGT+AGG - Chr3:48723016-48723035 None:intergenic 40.0%
!!! AACTGCTAGTTTTCGGATAC+GGG + Chr3:48723177-48723196 MsG0380014045.01.T01:CDS 40.0%
!!! GAACTGCTAGTTTTCGGATA+CGG + Chr3:48723176-48723195 MsG0380014045.01.T01:CDS 40.0%
AACGATCTTTCCTGCGTGTT+CGG - Chr3:48723711-48723730 None:intergenic 45.0%
AATCCTTGCGTGAATTGCCA+AGG - Chr3:48723278-48723297 None:intergenic 45.0%
ACCTCGGAAGGAATCAATGT+AGG + Chr3:48723241-48723260 MsG0380014045.01.T01:CDS 45.0%
CAAATGCGTCCATAGTGACT+AGG - Chr3:48723211-48723230 None:intergenic 45.0%
GTCAAGCAGTTCCTCTTTCT+CGG + Chr3:48723615-48723634 MsG0380014045.01.T01:CDS 45.0%
GTGCATTGTTGGAACTGGAT+TGG + Chr3:48723646-48723665 MsG0380014045.01.T01:CDS 45.0%
TTGAGTTATTTGGGTCCTGG+AGG + Chr3:48723142-48723161 MsG0380014045.01.T01:CDS 45.0%
TTGGCAATTCACGCAAGGAT+TGG + Chr3:48723277-48723296 MsG0380014045.01.T01:CDS 45.0%
! AATTCTTGACGGTATCGAGC+TGG - Chr3:48723461-48723480 None:intergenic 45.0%
! ACTGAATCGAGGTATTCAGG+AGG + Chr3:48723424-48723443 MsG0380014045.01.T01:CDS 45.0%
! AGCACTGAATCGAGGTATTC+AGG + Chr3:48723421-48723440 MsG0380014045.01.T01:CDS 45.0%
! ATTCCTTCCGAGGTGTCAAT+TGG - Chr3:48723235-48723254 None:intergenic 45.0%
!! TCCTACATTGATTCCTTCCG+AGG - Chr3:48723245-48723264 None:intergenic 45.0%
AGCGGGAACTCCTGAATCTA+AGG - Chr3:48723684-48723703 None:intergenic 50.0%
GATCCTTGGCAATTCACGCA+AGG + Chr3:48723272-48723291 MsG0380014045.01.T01:CDS 50.0%
GGACTTTCTATAGACCCCCA+AGG - Chr3:48723304-48723323 None:intergenic 50.0%
TGCGTCCATAGTGACTAGGA+TGG - Chr3:48723207-48723226 None:intergenic 50.0%
TGTCCAATTGACACCTCGGA+AGG + Chr3:48723229-48723248 MsG0380014045.01.T01:CDS 50.0%
TTTGGGTCCTGGAGGATTGA+CGG + Chr3:48723150-48723169 MsG0380014045.01.T01:CDS 50.0%
! AACTGGATTGGAACGCCAAG+TGG + Chr3:48723658-48723677 MsG0380014045.01.T01:CDS 50.0%
! CACTTTTCGGACCGAGAAAG+AGG - Chr3:48723629-48723648 None:intergenic 50.0%
! CGGTCCGAAAAGTGCATTGT+TGG + Chr3:48723635-48723654 MsG0380014045.01.T01:CDS 50.0%
! CTCCTGAATCTAAGGCCACT+TGG - Chr3:48723676-48723695 None:intergenic 50.0%
!! TCACGCAAGGATTGGTCCTT+GGG + Chr3:48723285-48723304 MsG0380014045.01.T01:CDS 50.0%
!! TTCACGCAAGGATTGGTCCT+TGG + Chr3:48723284-48723303 MsG0380014045.01.T01:CDS 50.0%
TCCTGCGTGTTCGGCTATAG+CGG - Chr3:48723702-48723721 None:intergenic 55.0%
TTGGGTCCTGGAGGATTGAC+GGG + Chr3:48723151-48723170 MsG0380014045.01.T01:CDS 55.0%
! CGCCAAGTGGCCTTAGATTC+AGG + Chr3:48723671-48723690 MsG0380014045.01.T01:CDS 55.0%
!! ACGCAAGGATTGGTCCTTGG+GGG + Chr3:48723287-48723306 MsG0380014045.01.T01:CDS 55.0%
!! CACGCAAGGATTGGTCCTTG+GGG + Chr3:48723286-48723305 MsG0380014045.01.T01:CDS 55.0%
!!! CGGGGCGAACTGCTAGTTTT+CGG + Chr3:48723170-48723189 MsG0380014045.01.T01:CDS 55.0%
CCTGCGTGTTCGGCTATAGC+GGG - Chr3:48723701-48723720 None:intergenic 60.0%
TGGGTCCTGGAGGATTGACG+GGG + Chr3:48723152-48723171 MsG0380014045.01.T01:CDS 60.0%
CCCGCTATAGCCGAACACGC+AGG + Chr3:48723698-48723717 MsG0380014045.01.T01:CDS 65.0%
GTTCGCCCCGTCAATCCTCC+AGG - Chr3:48723160-48723179 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48722851 48723778 48722851 ID=MsG0380014045.01;Name=MsG0380014045.01
Chr3 mRNA 48722851 48723778 48722851 ID=MsG0380014045.01.T01;Parent=MsG0380014045.01;Name=MsG0380014045.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|280
Chr3 exon 48722851 48723319 48722851 ID=MsG0380014045.01.T01:exon:8543;Parent=MsG0380014045.01.T01
Chr3 exon 48723405 48723778 48723405 ID=MsG0380014045.01.T01:exon:8544;Parent=MsG0380014045.01.T01
Chr3 CDS 48722851 48723319 48722851 ID=MsG0380014045.01.T01:cds;Parent=MsG0380014045.01.T01
Chr3 CDS 48723405 48723778 48723405 ID=MsG0380014045.01.T01:cds;Parent=MsG0380014045.01.T01
Gene Sequence

>MsG0380014045.01.T01

ATGAAATTTGGAATGGGTACACTTGACGATATGAATCATTTGAAAAATAAACGTATTCGTTCCGTAGCAGATCTATTACAAGATCAATTTGGATTGGCTCTCGTTCGTTTAGAAAATATAATTCGAGGAACTATATCTGGAGCAATTAGATATAAATTGATACCTACTCCTCAGAATTTAGTGACTTCAACTCCATTAACAACCACTTATGAATCGTTTTTTGGATTACACCCATTATCTCAAGTTTTAGATCGAACCAATCCATTGACCCAAATAGTTCATGGGAGAAAATTGAGTTATTTGGGTCCTGGAGGATTGACGGGGCGAACTGCTAGTTTTCGGATACGGGATATCCATCCTAGTCACTATGGACGCATTTGTCCAATTGACACCTCGGAAGGAATCAATGTAGGACTTATTGGATCCTTGGCAATTCACGCAAGGATTGGTCCTTGGGGGTCTATAGAAACGACGGGAAATTTTTTAGCACTGAATCGAGGTATTCAGGAGGAGCAGATTGTTCCAGCTCGATACCGTCAAGAATTTCTAACTATTGCATGGGAACAGGTTCATCTTCGAAGTATTTTTCCCTTCCAATATTTTTCTATTGGAGCTTCCCTGATTCCTTTTATCGAGCATAATGATGCGAATCGAGCTTTAATGAGTTCTAATATGCAACGTCAAGCAGTTCCTCTTTCTCGGTCCGAAAAGTGCATTGTTGGAACTGGATTGGAACGCCAAGTGGCCTTAGATTCAGGAGTTCCCGCTATAGCCGAACACGCAGGAAAGATCGTTTATAACGATACTGACAAGATCATTTATTTGGAAATGGGAATGCTCTAA

Protein sequence

>MsG0380014045.01.T01

MKFGMGTLDDMNHLKNKRIRSVADLLQDQFGLALVRLENIIRGTISGAIRYKLIPTPQNLVTSTPLTTTYESFFGLHPLSQVLDRTNPLTQIVHGRKLSYLGPGGLTGRTASFRIRDIHPSHYGRICPIDTSEGINVGLIGSLAIHARIGPWGSIETTGNFLALNRGIQEEQIVPARYRQEFLTIAWEQVHLRSIFPFQYFSIGASLIPFIEHNDANRALMSSNMQRQAVPLSRSEKCIVGTGLERQVALDSGVPAIAEHAGKIVYNDTDKIIYLEMGML*