AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037988.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037988.01.T01 MTR_7g033770 96.377 138 5 0 1 138 1 138 7.78e-94 269
MsG0780037988.01.T01 MTR_4g051270 97.101 138 4 0 1 138 1 138 7.36e-92 273
MsG0780037988.01.T01 MTR_7g021770 97.101 138 4 0 1 138 1 138 1.83e-91 273
MsG0780037988.01.T01 MTR_7g117930 96.377 138 5 0 1 138 1 138 9.19e-91 271
MsG0780037988.01.T01 MTR_6g055010 96.377 138 5 0 1 138 1 138 7.94e-90 269
MsG0780037988.01.T01 MTR_0002s0660 91.304 138 12 0 1 138 1 138 2.16e-78 239
MsG0780037988.01.T01 MTR_5g075460 42.056 107 51 4 42 138 17 122 1.49e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037988.01.T01 ATCG00490 91.304 138 12 0 1 138 1 138 7.88e-79 241

Find 35 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATTGTAGGTAATGTATT+TGG 0.076014 7:+37743622 MsG0780037988.01.T01:CDS
TGCCAAGATATCAGTATCTT+TGG 0.281178 7:-37743359 None:intergenic
ACCAGTCTTGATCGTTATAA+AGG 0.335616 7:+37743490 MsG0780037988.01.T01:CDS
CCATTGTAGGTAATGTATTT+GGG 0.341419 7:+37743623 MsG0780037988.01.T01:CDS
GTTGCTTTAGTTTCTGTTTG+TGG 0.353241 7:-37743274 None:intergenic
TAACAGAACCTTCTTCAAAA+AGG 0.354806 7:-37743585 None:intergenic
AAGTCCTCAACCTGGAGTTC+TGG 0.377883 7:+37743393 MsG0780037988.01.T01:CDS
GTATCTTTGGTTTCATAGTC+AGG 0.391019 7:-37743346 None:intergenic
AACCAAAGATACTGATATCT+TGG 0.402306 7:+37743357 MsG0780037988.01.T01:CDS
AGGTAATGTATTTGGGTTCA+AGG 0.415592 7:+37743630 MsG0780037988.01.T01:CDS
AACATGTTTACCTCCATTGT+AGG 0.424333 7:+37743610 MsG0780037988.01.T01:CDS
TGACTCTCTTCTCCAGCAAC+AGG 0.424738 7:-37743532 None:intergenic
GGCCTTGAGTGCTCTACGTC+TGG 0.427222 7:+37743651 MsG0780037988.01.T01:CDS
TACCACATCGAACCTGTTGC+TGG 0.448326 7:+37743520 MsG0780037988.01.T01:CDS
GCTTCTTCAGCCAGAACTCC+AGG 0.464840 7:-37743403 None:intergenic
GGAGTTCTGGCTGAAGAAGC+AGG 0.503845 7:+37743406 MsG0780037988.01.T01:CDS
CCCAAATACATTACCTACAA+TGG 0.508175 7:-37743623 None:intergenic
GCAACGGTTGGGTTCAAAGC+TGG 0.510113 7:+37743292 MsG0780037988.01.T01:CDS
CTGAATCTTCCACTGGGACA+TGG 0.521266 7:+37743443 MsG0780037988.01.T01:CDS
ACAGAAACTAAAGCAACGGT+TGG 0.522484 7:+37743280 MsG0780037988.01.T01:CDS
GTAGCTGCTGAATCTTCCAC+TGG 0.537280 7:+37743436 MsG0780037988.01.T01:CDS
TCCTTTATAACGATCAAGAC+TGG 0.551429 7:-37743491 None:intergenic
TCAGCCAGAACTCCAGGTTG+AGG 0.563203 7:-37743397 None:intergenic
TTCCAGACGTAGAGCACTCA+AGG 0.569208 7:-37743653 None:intergenic
CTCCAGGTTGAGGACTTACT+CGG 0.570888 7:-37743387 None:intergenic
CAGAAACTAAAGCAACGGTT+GGG 0.575347 7:+37743281 MsG0780037988.01.T01:CDS
ATCAAGACTGGTAAGTTCAT+CGG 0.580416 7:-37743479 None:intergenic
TTCCGAGTAAGTCCTCAACC+TGG 0.592101 7:+37743385 MsG0780037988.01.T01:CDS
GGCTGAAGAAGCAGGTGCAG+CGG 0.618937 7:+37743414 MsG0780037988.01.T01:CDS
AAATACATTACCTACAATGG+AGG 0.637677 7:-37743620 None:intergenic
ACAAACAGAAACTAAAGCAA+CGG 0.641511 7:+37743276 MsG0780037988.01.T01:CDS
CTCCAGCAACAGGTTCGATG+TGG 0.653396 7:-37743522 None:intergenic
CACAGTTGTCCATGTCCCAG+TGG 0.656758 7:-37743452 None:intergenic
TAGCTGCTGAATCTTCCACT+GGG 0.674504 7:+37743437 MsG0780037988.01.T01:CDS
CTGGGACATGGACAACTGTG+TGG 0.707072 7:+37743455 MsG0780037988.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAATACATTACCTACAATGG+AGG - Chr7:37743623-37743642 None:intergenic 30.0%
AACCAAAGATACTGATATCT+TGG + Chr7:37743357-37743376 MsG0780037988.01.T01:CDS 30.0%
ACAAACAGAAACTAAAGCAA+CGG + Chr7:37743276-37743295 MsG0780037988.01.T01:CDS 30.0%
CCATTGTAGGTAATGTATTT+GGG + Chr7:37743623-37743642 MsG0780037988.01.T01:CDS 30.0%
TAACAGAACCTTCTTCAAAA+AGG - Chr7:37743588-37743607 None:intergenic 30.0%
TCCATTGTAGGTAATGTATT+TGG + Chr7:37743622-37743641 MsG0780037988.01.T01:CDS 30.0%
AACATGTTTACCTCCATTGT+AGG + Chr7:37743610-37743629 MsG0780037988.01.T01:CDS 35.0%
ACCAGTCTTGATCGTTATAA+AGG + Chr7:37743490-37743509 MsG0780037988.01.T01:CDS 35.0%
ACCTTCTTCAAAAAGGTCTA+AGG - Chr7:37743581-37743600 None:intergenic 35.0%
ATCAAGACTGGTAAGTTCAT+CGG - Chr7:37743482-37743501 None:intergenic 35.0%
CCCAAATACATTACCTACAA+TGG - Chr7:37743626-37743645 None:intergenic 35.0%
CCTTCTTCAAAAAGGTCTAA+GGG - Chr7:37743580-37743599 None:intergenic 35.0%
TCCTTTATAACGATCAAGAC+TGG - Chr7:37743494-37743513 None:intergenic 35.0%
TGCCAAGATATCAGTATCTT+TGG - Chr7:37743362-37743381 None:intergenic 35.0%
! AGGTAATGTATTTGGGTTCA+AGG + Chr7:37743630-37743649 MsG0780037988.01.T01:CDS 35.0%
! GTATCTTTGGTTTCATAGTC+AGG - Chr7:37743349-37743368 None:intergenic 35.0%
! GTTGCTTTAGTTTCTGTTTG+TGG - Chr7:37743277-37743296 None:intergenic 35.0%
ACAGAAACTAAAGCAACGGT+TGG + Chr7:37743280-37743299 MsG0780037988.01.T01:CDS 40.0%
CAGAAACTAAAGCAACGGTT+GGG + Chr7:37743281-37743300 MsG0780037988.01.T01:CDS 40.0%
!! CCCTTAGACCTTTTTGAAGA+AGG + Chr7:37743577-37743596 MsG0780037988.01.T01:CDS 40.0%
TAGCTGCTGAATCTTCCACT+GGG + Chr7:37743437-37743456 MsG0780037988.01.T01:CDS 45.0%
AAGTCCTCAACCTGGAGTTC+TGG + Chr7:37743393-37743412 MsG0780037988.01.T01:CDS 50.0%
CTGAATCTTCCACTGGGACA+TGG + Chr7:37743443-37743462 MsG0780037988.01.T01:CDS 50.0%
GTAGCTGCTGAATCTTCCAC+TGG + Chr7:37743436-37743455 MsG0780037988.01.T01:CDS 50.0%
TACCACATCGAACCTGTTGC+TGG + Chr7:37743520-37743539 MsG0780037988.01.T01:CDS 50.0%
TGACTCTCTTCTCCAGCAAC+AGG - Chr7:37743535-37743554 None:intergenic 50.0%
TTCCGAGTAAGTCCTCAACC+TGG + Chr7:37743385-37743404 MsG0780037988.01.T01:CDS 50.0%
! CTCCAGGTTGAGGACTTACT+CGG - Chr7:37743390-37743409 None:intergenic 50.0%
! TTCCAGACGTAGAGCACTCA+AGG - Chr7:37743656-37743675 None:intergenic 50.0%
CACAGTTGTCCATGTCCCAG+TGG - Chr7:37743455-37743474 None:intergenic 55.0%
CTCCAGCAACAGGTTCGATG+TGG - Chr7:37743525-37743544 None:intergenic 55.0%
CTGGGACATGGACAACTGTG+TGG + Chr7:37743455-37743474 MsG0780037988.01.T01:CDS 55.0%
GCAACGGTTGGGTTCAAAGC+TGG + Chr7:37743292-37743311 MsG0780037988.01.T01:CDS 55.0%
GCTTCTTCAGCCAGAACTCC+AGG - Chr7:37743406-37743425 None:intergenic 55.0%
TCAGCCAGAACTCCAGGTTG+AGG - Chr7:37743400-37743419 None:intergenic 55.0%
! GGAGTTCTGGCTGAAGAAGC+AGG + Chr7:37743406-37743425 MsG0780037988.01.T01:CDS 55.0%
GGCCTTGAGTGCTCTACGTC+TGG + Chr7:37743651-37743670 MsG0780037988.01.T01:CDS 60.0%
GGCTGAAGAAGCAGGTGCAG+CGG + Chr7:37743414-37743433 MsG0780037988.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 37743268 37743684 37743268 ID=MsG0780037988.01;Name=MsG0780037988.01
Chr7 mRNA 37743268 37743684 37743268 ID=MsG0780037988.01.T01;Parent=MsG0780037988.01;Name=MsG0780037988.01.T01;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|138
Chr7 exon 37743268 37743684 37743268 ID=MsG0780037988.01.T01:exon:5611;Parent=MsG0780037988.01.T01
Chr7 CDS 37743268 37743684 37743268 ID=MsG0780037988.01.T01:cds;Parent=MsG0780037988.01.T01
Gene Sequence

>MsG0780037988.01.T01

ATGTCTCCACAAACAGAAACTAAAGCAACGGTTGGGTTCAAAGCTGGTGTTAAAGATTATAGATTGACTTATTATACTCCTGACTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAAGTCCTCAACCTGGAGTTCTGGCTGAAGAAGCAGGTGCAGCGGTAGCTGCTGAATCTTCCACTGGGACATGGACAACTGTGTGGACCGATGAACTTACCAGTCTTGATCGTTATAAAGGACACTGCTACCACATCGAACCTGTTGCTGGAGAAGAGAGTCAATTTATTGCTTATGTAGCTTATCCCTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACCTCCATTGTAGGTAATGTATTTGGGTTCAAGGCCTTGAGTGCTCTACGTCTGGAAGATTTGTGA

Protein sequence

>MsG0780037988.01.T01

MSPQTETKATVGFKAGVKDYRLTYYTPDYETKDTDILAAFRVSPQPGVLAEEAGAAVAAESSTGTWTTVWTDELTSLDRYKGHCYHIEPVAGEESQFIAYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALSALRLEDL*