Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014145.01.T01 | XP_004500060.1 | 98.601 | 143 | 2 | 0 | 1 | 143 | 551 | 693 | 9.23E-93 | 293 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014145.01.T01 | A0A1S2Y7G9 | 98.601 | 143 | 2 | 0 | 1 | 143 | 551 | 693 | 4.41e-93 | 293 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048809.01 | MsG0380014145.01 | 0.829979 | 3.800823e-55 | 3.951794e-52 |
| MsG0080048901.01 | MsG0380014145.01 | 0.812934 | 3.302467e-51 | 2.127270e-48 |
| MsG0280011419.01 | MsG0380014145.01 | 0.821169 | 4.656826e-53 | 3.760394e-50 |
| MsG0380014145.01 | MsG0380014551.01 | 0.823184 | 1.587466e-53 | 1.357102e-50 |
| MsG0380014145.01 | MsG0380016809.01 | 0.809820 | 1.566522e-50 | 9.291193e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014145.01.T01 | MTR_3g051430 | 97.902 | 143 | 3 | 0 | 1 | 143 | 553 | 695 | 6.20e-96 | 291 |
| MsG0380014145.01.T01 | MTR_5g092610 | 74.825 | 143 | 35 | 1 | 2 | 143 | 544 | 686 | 5.14e-68 | 218 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014145.01.T01 | AT1G50660 | 59.155 | 142 | 46 | 7 | 14 | 143 | 584 | 725 | 5.44e-40 | 142 |
| MsG0380014145.01.T01 | AT3G20350 | 50.355 | 141 | 53 | 5 | 8 | 143 | 545 | 673 | 4.07e-31 | 117 |
Find 51 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCACTTATTTCAGTTAA+TGG | 0.169469 | 3:+50337766 | None:intergenic |
| CTTCACTTATTTCAGTTAAT+GGG | 0.249830 | 3:+50337767 | None:intergenic |
| CAGTTAAGTTCTCCCGATTC+AGG | 0.275240 | 3:-50337535 | MsG0380014145.01.T01:CDS |
| CCTTTATCAAGTCCCCATTC+AGG | 0.359947 | 3:+50337586 | None:intergenic |
| TATCTTGTAATTGTCCTCAT+TGG | 0.393252 | 3:+50337675 | None:intergenic |
| TAACTGGCATAAAATGTCTT+GGG | 0.395169 | 3:+50337552 | None:intergenic |
| ATTATGCACGCTTCCTGAAT+CGG | 0.399262 | 3:+50337522 | None:intergenic |
| TAGTTTGAAAGCAAGACTTA+TGG | 0.399494 | 3:-50337458 | MsG0380014145.01.T01:CDS |
| ATTCGCTTCGATTGCTTCGT+TGG | 0.401778 | 3:+50337733 | None:intergenic |
| CAATTACAAGATAATCTCTA+TGG | 0.402648 | 3:-50337665 | MsG0380014145.01.T01:CDS |
| CCTGAATGGGGACTTGATAA+AGG | 0.403248 | 3:-50337586 | MsG0380014145.01.T01:CDS |
| CTGAATCGGGAGAACTTAAC+TGG | 0.405141 | 3:+50337536 | None:intergenic |
| TCAAATGGAAAGGTATCTAA+TGG | 0.422968 | 3:-50337622 | MsG0380014145.01.T01:CDS |
| ATAGCTAGGCTTTGGAGGTC+AGG | 0.428537 | 3:-50337703 | MsG0380014145.01.T01:CDS |
| CGGGGATGAGTGTGATAGAA+TGG | 0.432179 | 3:-50337813 | None:intergenic |
| TTATGCACGCTTCCTGAATC+GGG | 0.441236 | 3:+50337523 | None:intergenic |
| TATCATCCATAGCTAGGCTT+TGG | 0.448915 | 3:-50337711 | MsG0380014145.01.T01:CDS |
| TTAACTGGCATAAAATGTCT+TGG | 0.453586 | 3:+50337551 | None:intergenic |
| CATCCATAGCTAGGCTTTGG+AGG | 0.461831 | 3:-50337708 | MsG0380014145.01.T01:CDS |
| TTGTAATTGTCCTCATTGGT+TGG | 0.482063 | 3:+50337679 | None:intergenic |
| TGGCATCATGTCTCCTGAAT+GGG | 0.490175 | 3:-50337599 | MsG0380014145.01.T01:CDS |
| CTTGCTTTCAAACTACCCTT+CGG | 0.491855 | 3:+50337466 | None:intergenic |
| ATCTCTATGGAGGGAATGAA+CGG | 0.506340 | 3:-50337652 | MsG0380014145.01.T01:CDS |
| TCTCTATGGAGGGAATGAAC+GGG | 0.520215 | 3:-50337651 | MsG0380014145.01.T01:CDS |
| AAATCTTCTGTTTCAGAACA+TGG | 0.525509 | 3:+50337401 | None:intergenic |
| TGACCTCCAAAGCCTAGCTA+TGG | 0.541253 | 3:+50337705 | None:intergenic |
| ATGAACGGGAGACTTTCAAA+TGG | 0.547140 | 3:-50337637 | MsG0380014145.01.T01:CDS |
| TTCACTTATTTCAGTTAATG+GGG | 0.553559 | 3:+50337768 | None:intergenic |
| GGGGATGAGTGTGATAGAAT+GGG | 0.554217 | 3:-50337812 | None:intergenic |
| GTGGCATCATGTCTCCTGAA+TGG | 0.556230 | 3:-50337600 | MsG0380014145.01.T01:CDS |
| TTACAAGATAATCTCTATGG+AGG | 0.556931 | 3:-50337662 | MsG0380014145.01.T01:CDS |
| CGGGAGACTTTCAAATGGAA+AGG | 0.557469 | 3:-50337632 | MsG0380014145.01.T01:CDS |
| CATTCCTCGCACAGGACCGA+AGG | 0.559925 | 3:-50337482 | MsG0380014145.01.T01:CDS |
| AAGCAAGACTTATGGAGGCT+AGG | 0.565845 | 3:-50337450 | MsG0380014145.01.T01:CDS |
| AACTGGCATAAAATGTCTTG+GGG | 0.580797 | 3:+50337553 | None:intergenic |
| ATAGAATGGGAAGGAAATGC+AGG | 0.582843 | 3:-50337799 | MsG0380014145.01.T01:CDS |
| GGCATCATGTCTCCTGAATG+GGG | 0.584389 | 3:-50337598 | MsG0380014145.01.T01:CDS |
| AGCGAATATCATCCATAGCT+AGG | 0.587566 | 3:-50337717 | MsG0380014145.01.T01:CDS |
| TTTGAAAGCAAGACTTATGG+AGG | 0.593390 | 3:-50337455 | MsG0380014145.01.T01:CDS |
| GTGCATAATCGAGGCATGAA+AGG | 0.599317 | 3:-50337508 | MsG0380014145.01.T01:CDS |
| AAAGGATGCATTCCTCGCAC+AGG | 0.599405 | 3:-50337490 | MsG0380014145.01.T01:CDS |
| CTACCCTTCGGTCCTGTGCG+AGG | 0.606504 | 3:+50337478 | None:intergenic |
| TACAAGATAATCTCTATGGA+GGG | 0.607094 | 3:-50337661 | MsG0380014145.01.T01:CDS |
| TCAGGAAGCGTGCATAATCG+AGG | 0.611921 | 3:-50337517 | MsG0380014145.01.T01:CDS |
| AATGGAAAGGTATCTAATGG+TGG | 0.631991 | 3:-50337619 | MsG0380014145.01.T01:CDS |
| ATTCCTCGCACAGGACCGAA+GGG | 0.653123 | 3:-50337481 | MsG0380014145.01.T01:CDS |
| GAATGGGGACTTGATAAAGG+TGG | 0.654198 | 3:-50337583 | MsG0380014145.01.T01:CDS |
| ACTGGCATAAAATGTCTTGG+GGG | 0.656958 | 3:+50337554 | None:intergenic |
| ATGAGTGTGATAGAATGGGA+AGG | 0.657189 | 3:-50337808 | MsG0380014145.01.T01:CDS |
| GAGGTCAGGACCAACCAATG+AGG | 0.694089 | 3:-50337689 | MsG0380014145.01.T01:CDS |
| GGCTAGGATAGAAAGCAACA+AGG | 0.714877 | 3:-50337434 | MsG0380014145.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTTCACTTATTTCAGTTAA+TGG | + | Chr3:50337444-50337463 | None:intergenic | 25.0% |
| ! | CAATTACAAGATAATCTCTA+TGG | - | Chr3:50337542-50337561 | MsG0380014145.01.T01:CDS | 25.0% |
| ! | CTTCACTTATTTCAGTTAAT+GGG | + | Chr3:50337443-50337462 | None:intergenic | 25.0% |
| ! | TTCACTTATTTCAGTTAATG+GGG | + | Chr3:50337442-50337461 | None:intergenic | 25.0% |
| AAATCTTCTGTTTCAGAACA+TGG | + | Chr3:50337809-50337828 | None:intergenic | 30.0% | |
| TAACTGGCATAAAATGTCTT+GGG | + | Chr3:50337658-50337677 | None:intergenic | 30.0% | |
| TACAAGATAATCTCTATGGA+GGG | - | Chr3:50337546-50337565 | MsG0380014145.01.T01:CDS | 30.0% | |
| TAGTTTGAAAGCAAGACTTA+TGG | - | Chr3:50337749-50337768 | MsG0380014145.01.T01:CDS | 30.0% | |
| TATCTTGTAATTGTCCTCAT+TGG | + | Chr3:50337535-50337554 | None:intergenic | 30.0% | |
| TTAACTGGCATAAAATGTCT+TGG | + | Chr3:50337659-50337678 | None:intergenic | 30.0% | |
| TTACAAGATAATCTCTATGG+AGG | - | Chr3:50337545-50337564 | MsG0380014145.01.T01:CDS | 30.0% | |
| !! | TCAAATGGAAAGGTATCTAA+TGG | - | Chr3:50337585-50337604 | MsG0380014145.01.T01:CDS | 30.0% |
| AACTGGCATAAAATGTCTTG+GGG | + | Chr3:50337657-50337676 | None:intergenic | 35.0% | |
| TTGTAATTGTCCTCATTGGT+TGG | + | Chr3:50337531-50337550 | None:intergenic | 35.0% | |
| TTTGAAAGCAAGACTTATGG+AGG | - | Chr3:50337752-50337771 | MsG0380014145.01.T01:CDS | 35.0% | |
| !! | AATGGAAAGGTATCTAATGG+TGG | - | Chr3:50337588-50337607 | MsG0380014145.01.T01:CDS | 35.0% |
| ACTGGCATAAAATGTCTTGG+GGG | + | Chr3:50337656-50337675 | None:intergenic | 40.0% | |
| AGCGAATATCATCCATAGCT+AGG | - | Chr3:50337490-50337509 | MsG0380014145.01.T01:CDS | 40.0% | |
| ATAGAATGGGAAGGAAATGC+AGG | - | Chr3:50337408-50337427 | MsG0380014145.01.T01:CDS | 40.0% | |
| ATCTCTATGGAGGGAATGAA+CGG | - | Chr3:50337555-50337574 | MsG0380014145.01.T01:CDS | 40.0% | |
| ATGAACGGGAGACTTTCAAA+TGG | - | Chr3:50337570-50337589 | MsG0380014145.01.T01:CDS | 40.0% | |
| ATGAGTGTGATAGAATGGGA+AGG | - | Chr3:50337399-50337418 | MsG0380014145.01.T01:CDS | 40.0% | |
| ATTATGCACGCTTCCTGAAT+CGG | + | Chr3:50337688-50337707 | None:intergenic | 40.0% | |
| CTTGCTTTCAAACTACCCTT+CGG | + | Chr3:50337744-50337763 | None:intergenic | 40.0% | |
| TATCATCCATAGCTAGGCTT+TGG | - | Chr3:50337496-50337515 | MsG0380014145.01.T01:CDS | 40.0% | |
| AAGCAAGACTTATGGAGGCT+AGG | - | Chr3:50337757-50337776 | MsG0380014145.01.T01:CDS | 45.0% | |
| ATTCGCTTCGATTGCTTCGT+TGG | + | Chr3:50337477-50337496 | None:intergenic | 45.0% | |
| CAGTTAAGTTCTCCCGATTC+AGG | - | Chr3:50337672-50337691 | MsG0380014145.01.T01:CDS | 45.0% | |
| CCTGAATGGGGACTTGATAA+AGG | - | Chr3:50337621-50337640 | MsG0380014145.01.T01:CDS | 45.0% | |
| CCTTTATCAAGTCCCCATTC+AGG | + | Chr3:50337624-50337643 | None:intergenic | 45.0% | |
| CGGGAGACTTTCAAATGGAA+AGG | - | Chr3:50337575-50337594 | MsG0380014145.01.T01:CDS | 45.0% | |
| CTGAATCGGGAGAACTTAAC+TGG | + | Chr3:50337674-50337693 | None:intergenic | 45.0% | |
| GAATGGGGACTTGATAAAGG+TGG | - | Chr3:50337624-50337643 | MsG0380014145.01.T01:CDS | 45.0% | |
| GGCTAGGATAGAAAGCAACA+AGG | - | Chr3:50337773-50337792 | MsG0380014145.01.T01:CDS | 45.0% | |
| GTGCATAATCGAGGCATGAA+AGG | - | Chr3:50337699-50337718 | MsG0380014145.01.T01:CDS | 45.0% | |
| TCTCTATGGAGGGAATGAAC+GGG | - | Chr3:50337556-50337575 | MsG0380014145.01.T01:CDS | 45.0% | |
| TGGCATCATGTCTCCTGAAT+GGG | - | Chr3:50337608-50337627 | MsG0380014145.01.T01:CDS | 45.0% | |
| TTATGCACGCTTCCTGAATC+GGG | + | Chr3:50337687-50337706 | None:intergenic | 45.0% | |
| AAAGGATGCATTCCTCGCAC+AGG | - | Chr3:50337717-50337736 | MsG0380014145.01.T01:CDS | 50.0% | |
| GGCATCATGTCTCCTGAATG+GGG | - | Chr3:50337609-50337628 | MsG0380014145.01.T01:CDS | 50.0% | |
| GTGGCATCATGTCTCCTGAA+TGG | - | Chr3:50337607-50337626 | MsG0380014145.01.T01:CDS | 50.0% | |
| TGACCTCCAAAGCCTAGCTA+TGG | + | Chr3:50337505-50337524 | None:intergenic | 50.0% | |
| ! | CATCCATAGCTAGGCTTTGG+AGG | - | Chr3:50337499-50337518 | MsG0380014145.01.T01:CDS | 50.0% |
| ! | TCAGGAAGCGTGCATAATCG+AGG | - | Chr3:50337690-50337709 | MsG0380014145.01.T01:CDS | 50.0% |
| !! | ATAGCTAGGCTTTGGAGGTC+AGG | - | Chr3:50337504-50337523 | MsG0380014145.01.T01:CDS | 50.0% |
| ATTCCTCGCACAGGACCGAA+GGG | - | Chr3:50337726-50337745 | MsG0380014145.01.T01:CDS | 55.0% | |
| GAGGTCAGGACCAACCAATG+AGG | - | Chr3:50337518-50337537 | MsG0380014145.01.T01:CDS | 55.0% | |
| CATTCCTCGCACAGGACCGA+AGG | - | Chr3:50337725-50337744 | MsG0380014145.01.T01:CDS | 60.0% | |
| CTACCCTTCGGTCCTGTGCG+AGG | + | Chr3:50337732-50337751 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 50337399 | 50337830 | 50337399 | ID=MsG0380014145.01;Name=MsG0380014145.01 |
| Chr3 | mRNA | 50337399 | 50337830 | 50337399 | ID=MsG0380014145.01.T01;Parent=MsG0380014145.01;Name=MsG0380014145.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|143 |
| Chr3 | exon | 50337399 | 50337830 | 50337399 | ID=MsG0380014145.01.T01:exon:9196;Parent=MsG0380014145.01.T01 |
| Chr3 | CDS | 50337399 | 50337830 | 50337399 | ID=MsG0380014145.01.T01:cds;Parent=MsG0380014145.01.T01 |
| Gene Sequence |
| Protein sequence |
