AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014551.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014551.01.T01 MTR_3g060200 44.275 131 51 2 2 110 45 175 1.04e-25 98.6
MsG0380014551.01.T01 MTR_3g100040 50.000 106 31 1 3 86 94 199 6.76e-25 95.1
MsG0380014551.01.T01 MTR_3g100040 50.000 106 31 1 3 86 198 303 1.59e-24 95.9
MsG0380014551.01.T01 MTR_3g060200 65.000 60 21 0 2 61 45 104 1.64e-18 79.7
MsG0380014551.01.T01 MTR_3g060200 65.000 60 21 0 2 61 45 104 1.74e-18 80.1
MsG0380014551.01.T01 MTR_3g060200 65.000 60 21 0 2 61 45 104 1.76e-18 79.7
MsG0380014551.01.T01 MTR_4g093730 46.512 86 42 3 2 84 172 256 2.10e-15 70.9
MsG0380014551.01.T01 MTR_3g100050 40.698 86 48 2 1 86 194 276 1.15e-13 65.9
MsG0380014551.01.T01 MTR_5g066510 40.964 83 42 2 7 86 203 281 2.03e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014551.01.T01 AT1G51600 62.791 43 16 0 7 49 223 265 2.68e-13 64.7
MsG0380014551.01.T01 AT1G51600 62.791 43 16 0 7 49 223 265 2.68e-13 64.7
MsG0380014551.01.T01 AT3G21175 62.791 43 16 0 7 49 219 261 1.61e-12 62.8
MsG0380014551.01.T01 AT3G21175 62.791 43 16 0 7 49 217 259 1.63e-12 62.8
MsG0380014551.01.T01 AT4G24470 43.056 72 36 1 7 78 214 280 1.95e-12 62.4
MsG0380014551.01.T01 AT4G24470 43.056 72 36 1 7 78 214 280 1.95e-12 62.4
MsG0380014551.01.T01 AT4G24470 56.818 44 19 0 7 50 214 257 1.35e-11 60.1

Find 24 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCGTCACGTGGGCCAAAA+AGG 0.305959 3:+56419773 MsG0380014551.01.T01:CDS
TTTAAAACTACCTCTTCAAC+GGG 0.359063 3:-56421515 None:intergenic
CAAATGCGTCCCGGGCCAGC+TGG 0.413545 3:+56419726 MsG0380014551.01.T01:CDS
AGTAAATCTTCCAGCTGGCC+CGG 0.440593 3:-56419736 None:intergenic
TTCTGTTGATCAAAGTGGTC+TGG 0.446513 3:+56420294 MsG0380014551.01.T01:CDS
AGTGGCAGAACACGGCCAAT+TGG 0.462481 3:-56421563 None:intergenic
TCCAAGCTCATTGTTGCCGA+TGG 0.464599 3:+56421470 MsG0380014551.01.T01:CDS
ATTTAAAACTACCTCTTCAA+CGG 0.506639 3:-56421516 None:intergenic
ACCATCGGCAACAATGAGCT+TGG 0.511631 3:-56421471 None:intergenic
AACACTTCTCAAATGCGTCC+CGG 0.512353 3:+56419717 MsG0380014551.01.T01:CDS
TGCATGATTGTTCGAACCAT+CGG 0.515819 3:-56421486 None:intergenic
TCGGCAACAATGAGCTTGGA+AGG 0.523449 3:-56421467 None:intergenic
ACACTTCTCAAATGCGTCCC+GGG 0.528359 3:+56419718 MsG0380014551.01.T01:CDS
TGCATTGAACCCCGTTGAAG+AGG 0.538463 3:+56421505 MsG0380014551.01.T01:CDS
GTAAATCTTCCAGCTGGCCC+GGG 0.542997 3:-56419735 None:intergenic
ACTCATTCTGTTGATCAAAG+TGG 0.569763 3:+56420289 MsG0380014551.01.T01:CDS
CATAGAGTAAATCTTCCAGC+TGG 0.585448 3:-56419741 None:intergenic
TACTTAAAAGTGGCAGAACA+CGG 0.589574 3:-56421571 None:intergenic
GTAATACATGCGGCGTCACG+TGG 0.612083 3:+56419763 MsG0380014551.01.T01:CDS
AGACGTAGATGTCAACATTG+TGG 0.642693 3:+56419681 MsG0380014551.01.T01:CDS
CGGCAACAATGAGCTTGGAA+GGG 0.643164 3:-56421466 None:intergenic
TTTACTCTATGTAATACATG+CGG 0.695440 3:+56419753 MsG0380014551.01.T01:CDS
TAATACATGCGGCGTCACGT+GGG 0.699536 3:+56419764 MsG0380014551.01.T01:CDS
TTAAAACTACCTCTTCAACG+GGG 0.758088 3:-56421514 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTTGTTAATAAATAAAA+GGG - Chr3:56420663-56420682 None:intergenic 10.0%
!! TTACTTTGTTAATAAATAAA+AGG - Chr3:56420664-56420683 None:intergenic 10.0%
!!! TTTTTGTCTTTATTTTATAT+TGG + Chr3:56421397-56421416 MsG0380014551.01.T01:intron 10.0%
!! AAAAAAATTATGCTATGTTC+AGG + Chr3:56421430-56421449 MsG0380014551.01.T01:intron 20.0%
!! AAAAAATTATGCTATGTTCA+GGG + Chr3:56421431-56421450 MsG0380014551.01.T01:intron 20.0%
!! AAAATAATGTTGAATGCAAA+TGG - Chr3:56421252-56421271 None:intergenic 20.0%
!! AACTCTACAAGTAATTAAAA+TGG - Chr3:56421337-56421356 None:intergenic 20.0%
!! TACAAGTAATTAAAATGGAA+AGG - Chr3:56421332-56421351 None:intergenic 20.0%
!!! AAAGTTTTACTTGTTCATTT+TGG - Chr3:56421189-56421208 None:intergenic 20.0%
!!! ACCAATTCATAAATTTTGAA+AGG + Chr3:56421058-56421077 MsG0380014551.01.T01:intron 20.0%
!!! CTCATGATTTATTTTATAGT+TGG + Chr3:56420344-56420363 MsG0380014551.01.T01:intron 20.0%
!!! GCATAATTTTTTTTTGCAAA+TGG - Chr3:56421423-56421442 None:intergenic 20.0%
!!! TCACAAAATACTTTATTTCA+TGG - Chr3:56420706-56420725 None:intergenic 20.0%
! AATAGTTATATTTGCAAACG+CGG - Chr3:56420209-56420228 None:intergenic 25.0%
! ACACAATAACATCATTTATG+TGG - Chr3:56419889-56419908 None:intergenic 25.0%
! ACACGTCATAATTCTTTAAT+TGG - Chr3:56420735-56420754 None:intergenic 25.0%
! ACATAATCTGTTCAATCATT+TGG + Chr3:56421303-56421322 MsG0380014551.01.T01:intron 25.0%
! CATTATAATGCTTATGAACT+TGG - Chr3:56421368-56421387 None:intergenic 25.0%
! GCCTTTCAAAATTTATGAAT+TGG - Chr3:56421062-56421081 None:intergenic 25.0%
! GCTAGAATAAAAAAGAAGTT+TGG + Chr3:56419912-56419931 MsG0380014551.01.T01:intron 25.0%
! TGAAATATAATGAAGGACAA+TGG - Chr3:56420073-56420092 None:intergenic 25.0%
! TGCATCATGAAATATAATGA+AGG - Chr3:56420080-56420099 None:intergenic 25.0%
! TTTACTCTATGTAATACATG+CGG + Chr3:56419753-56419772 MsG0380014551.01.T01:CDS 25.0%
!! AACAAAATGACTAACCTTTT+TGG - Chr3:56419790-56419809 None:intergenic 25.0%
!! ATTTAAAACTACCTCTTCAA+CGG - Chr3:56421519-56421538 None:intergenic 25.0%
!! GTTTTGTCAATACTTAAAAG+TGG - Chr3:56421584-56421603 None:intergenic 25.0%
!!! AAATGCTTTTGAAAGGATTA+AGG - Chr3:56421004-56421023 None:intergenic 25.0%
!!! AACGGATTGTTAAAATTGTT+TGG - Chr3:56420405-56420424 None:intergenic 25.0%
!!! AGTCATTTTGTTAACAAGTA+TGG + Chr3:56419798-56419817 MsG0380014551.01.T01:intron 25.0%
!!! GTTTTTTTTTAAACTCTGCA+GGG + Chr3:56420235-56420254 MsG0380014551.01.T01:intron 25.0%
!!! TGCATTTTGATATAATATGC+TGG + Chr3:56420096-56420115 MsG0380014551.01.T01:intron 25.0%
!!! TGTTTTGAAATGCTTTTGAA+AGG - Chr3:56421011-56421030 None:intergenic 25.0%
!!! TTATCTGCTTGTACTTTTTT+TGG + Chr3:56420853-56420872 MsG0380014551.01.T01:intron 25.0%
!!! TTTTTTTTTAAACTCTGCAG+GGG + Chr3:56420236-56420255 MsG0380014551.01.T01:intron 25.0%
ATCCTGCAAATTATATTCAG+TGG - Chr3:56421156-56421175 None:intergenic 30.0%
ATTTCATATCCAGTTCTTTG+TGG + Chr3:56420920-56420939 MsG0380014551.01.T01:intron 30.0%
GCAAATTATATTCAGTGGAT+AGG - Chr3:56421151-56421170 None:intergenic 30.0%
TATGGTTTCAATGAAATGCT+TGG + Chr3:56419816-56419835 MsG0380014551.01.T01:intron 30.0%
TTAAATCTTCCACAAAGAAC+TGG - Chr3:56420932-56420951 None:intergenic 30.0%
TTTATGTGGTTAGTACTTAG+TGG - Chr3:56419875-56419894 None:intergenic 30.0%
! ACAATCCGTTTGAAATCAAA+TGG + Chr3:56420415-56420434 MsG0380014551.01.T01:intron 30.0%
! ATCCACTGAATATAATTTGC+AGG + Chr3:56421151-56421170 MsG0380014551.01.T01:intron 30.0%
! CAACACCATTTGATTTCAAA+CGG - Chr3:56420423-56420442 None:intergenic 30.0%
! TTCATTCTTTTACAGCCAAT+TGG + Chr3:56421548-56421567 MsG0380014551.01.T01:CDS 30.0%
! TTTAAAACTACCTCTTCAAC+GGG - Chr3:56421518-56421537 None:intergenic 30.0%
!! TGGTTTATTTTCCCCTTATT+GGG - Chr3:56421042-56421061 None:intergenic 30.0%
!! TTGGTTTATTTTCCCCTTAT+TGG - Chr3:56421043-56421062 None:intergenic 30.0%
!!! CGTTTTTTTTTAAACTCTGC+AGG + Chr3:56420234-56420253 MsG0380014551.01.T01:intron 30.0%
AAAATAATACCAGTTGGAGG+AGG + Chr3:56420568-56420587 MsG0380014551.01.T01:intron 35.0%
AAACAATCTAACCCCCAATA+AGG + Chr3:56421026-56421045 MsG0380014551.01.T01:intron 35.0%
AACAATCTAACCCCCAATAA+GGG + Chr3:56421027-56421046 MsG0380014551.01.T01:intron 35.0%
ACTCATTCTGTTGATCAAAG+TGG + Chr3:56420289-56420308 MsG0380014551.01.T01:CDS 35.0%
ATCACGAATGCGAAGTAAAA+AGG - Chr3:56420139-56420158 None:intergenic 35.0%
GAAGGGAAAATAATACCAGT+TGG + Chr3:56420562-56420581 MsG0380014551.01.T01:intron 35.0%
TACTTAAAAGTGGCAGAACA+CGG - Chr3:56421574-56421593 None:intergenic 35.0%
TTAAAACTACCTCTTCAACG+GGG - Chr3:56421517-56421536 None:intergenic 35.0%
TTAGTAGAGAATGCAGAGAA+GGG + Chr3:56420545-56420564 MsG0380014551.01.T01:intron 35.0%
TTATATTATCCTCCTCCAAC+TGG - Chr3:56420580-56420599 None:intergenic 35.0%
TTTAGTAGAGAATGCAGAGA+AGG + Chr3:56420544-56420563 MsG0380014551.01.T01:intron 35.0%
! ACTTGTTCATTTTGGATGAC+AGG - Chr3:56421181-56421200 None:intergenic 35.0%
! AGGAAGCATAATCTCTTATC+AGG + Chr3:56420506-56420525 MsG0380014551.01.T01:intron 35.0%
! CGTTTGAAATCAAATGGTGT+TGG + Chr3:56420421-56420440 MsG0380014551.01.T01:intron 35.0%
! GGAAGCATAATCTCTTATCA+GGG + Chr3:56420507-56420526 MsG0380014551.01.T01:intron 35.0%
! TTACTCAAGTGCTTTTGGAT+AGG + Chr3:56420481-56420500 MsG0380014551.01.T01:intron 35.0%
! TTCAGTGGATAGGATGTTTT+AGG - Chr3:56421141-56421160 None:intergenic 35.0%
!! AAATCAAATGGTGTTGGTGA+TGG + Chr3:56420427-56420446 MsG0380014551.01.T01:intron 35.0%
!! GGTTTATTTTCCCCTTATTG+GGG - Chr3:56421041-56421060 None:intergenic 35.0%
!! GTTTATTTTCCCCTTATTGG+GGG - Chr3:56421040-56421059 None:intergenic 35.0%
!!! CTGCTTGTACTTTTTTTGGA+TGG + Chr3:56420857-56420876 MsG0380014551.01.T01:intron 35.0%
ACAATCTAACCCCCAATAAG+GGG + Chr3:56421028-56421047 MsG0380014551.01.T01:intron 40.0%
AGACGTAGATGTCAACATTG+TGG + Chr3:56419681-56419700 MsG0380014551.01.T01:CDS 40.0%
AGCAAACAAGTATGGTCAAC+AGG - Chr3:56420163-56420182 None:intergenic 40.0%
CACGTCAATAACTTGACGTA+TGG - Chr3:56420612-56420631 None:intergenic 40.0%
CATAGAGTAAATCTTCCAGC+TGG - Chr3:56419744-56419763 None:intergenic 40.0%
CCAGTTGCAAAAAGCTTAGT+GGG + Chr3:56420370-56420389 MsG0380014551.01.T01:intron 40.0%
GAGAATGCAGCAAACAAGTA+TGG - Chr3:56420171-56420190 None:intergenic 40.0%
GGGAAAATAATACCAGTTGG+AGG + Chr3:56420565-56420584 MsG0380014551.01.T01:intron 40.0%
TGCATGATTGTTCGAACCAT+CGG - Chr3:56421489-56421508 None:intergenic 40.0%
TTCTGTTGATCAAAGTGGTC+TGG + Chr3:56420294-56420313 MsG0380014551.01.T01:CDS 40.0%
! CAAGTGCTTTTGGATAGGAA+AGG + Chr3:56420486-56420505 MsG0380014551.01.T01:intron 40.0%
! GTGTCTTACTCAAGTGCTTT+TGG + Chr3:56420476-56420495 MsG0380014551.01.T01:intron 40.0%
! TTTTGGATGGCACAATGAGA+TGG + Chr3:56420870-56420889 MsG0380014551.01.T01:intron 40.0%
AACACTTCTCAAATGCGTCC+CGG + Chr3:56419717-56419736 MsG0380014551.01.T01:CDS 45.0%
GCCAGTTGCAAAAAGCTTAG+TGG + Chr3:56420369-56420388 MsG0380014551.01.T01:intron 45.0%
GCCTTACTTGAAAGTCATCG+TGG + Chr3:56421080-56421099 MsG0380014551.01.T01:intron 45.0%
! CCCACTAAGCTTTTTGCAAC+TGG - Chr3:56420373-56420392 None:intergenic 45.0%
! GCCACGATGACTTTCAAGTA+AGG - Chr3:56421084-56421103 None:intergenic 45.0%
ACACTTCTCAAATGCGTCCC+GGG + Chr3:56419718-56419737 MsG0380014551.01.T01:CDS 50.0%
ACCATCGGCAACAATGAGCT+TGG - Chr3:56421474-56421493 None:intergenic 50.0%
AGTAAATCTTCCAGCTGGCC+CGG - Chr3:56419739-56419758 None:intergenic 50.0%
CGGCAACAATGAGCTTGGAA+GGG - Chr3:56421469-56421488 None:intergenic 50.0%
TAATACATGCGGCGTCACGT+GGG + Chr3:56419764-56419783 MsG0380014551.01.T01:CDS 50.0%
TCCAAGCTCATTGTTGCCGA+TGG + Chr3:56421470-56421489 MsG0380014551.01.T01:CDS 50.0%
TCGGCAACAATGAGCTTGGA+AGG - Chr3:56421470-56421489 None:intergenic 50.0%
TGCATTGAACCCCGTTGAAG+AGG + Chr3:56421505-56421524 MsG0380014551.01.T01:CDS 50.0%
AGTGGCAGAACACGGCCAAT+TGG - Chr3:56421566-56421585 None:intergenic 55.0%
GTAAATCTTCCAGCTGGCCC+GGG - Chr3:56419738-56419757 None:intergenic 55.0%
GTAATACATGCGGCGTCACG+TGG + Chr3:56419763-56419782 MsG0380014551.01.T01:CDS 55.0%
CGGCGTCACGTGGGCCAAAA+AGG + Chr3:56419773-56419792 MsG0380014551.01.T01:CDS 65.0%
CAAATGCGTCCCGGGCCAGC+TGG + Chr3:56419726-56419745 MsG0380014551.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 56419672 56421628 56419672 ID=MsG0380014551.01;Name=MsG0380014551.01
Chr3 mRNA 56419672 56421628 56419672 ID=MsG0380014551.01.T01;Parent=MsG0380014551.01;Name=MsG0380014551.01.T01;_AED=0.49;_eAED=0.62;_QI=0|1|0.33|1|1|1|3|0|119
Chr3 exon 56419672 56419794 56419672 ID=MsG0380014551.01.T01:exon:23680;Parent=MsG0380014551.01.T01
Chr3 exon 56420256 56420315 56420256 ID=MsG0380014551.01.T01:exon:23681;Parent=MsG0380014551.01.T01
Chr3 exon 56421452 56421628 56421452 ID=MsG0380014551.01.T01:exon:23682;Parent=MsG0380014551.01.T01
Chr3 CDS 56419672 56419794 56419672 ID=MsG0380014551.01.T01:cds;Parent=MsG0380014551.01.T01
Chr3 CDS 56420256 56420315 56420256 ID=MsG0380014551.01.T01:cds;Parent=MsG0380014551.01.T01
Chr3 CDS 56421452 56421628 56421452 ID=MsG0380014551.01.T01:cds;Parent=MsG0380014551.01.T01
Gene Sequence

>MsG0380014551.01.T01

ATGTCTTCTAGACGTAGATGTCAACATTGTGGACTCGACGAAAATAACACTTCTCAAATGCGTCCCGGGCCAGCTGGAAGATTTACTCTATGTAATACATGCGGCGTCACGTGGGCCAAAAAGGGGATACTTAGAGATCTCAGTAAGAGACGAAGAACTCATTCTGTTGATCAAAGTGGTCTGGGTATTTCTGAAGACCCTTCCAAGCTCATTGTTGCCGATGGTTCGAACAATCATGCATTGAACCCCGTTGAAGAGGTAGTTTTAAATTTGTTTTTCTTCATTCTTTTACAGCCAATTGGCCGTGTTCTGCCACTTTTAAGTATTGACAAAACAAAAGTTTCTATGAGTAGTTTATGA

Protein sequence

>MsG0380014551.01.T01

MSSRRRCQHCGLDENNTSQMRPGPAGRFTLCNTCGVTWAKKGILRDLSKRRRTHSVDQSGLGISEDPSKLIVADGSNNHALNPVEEVVLNLFFFILLQPIGRVLPLLSIDKTKVSMSSL*