Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014303.01.T01 | ACJ84463.1 | 90.323 | 124 | 12 | 0 | 5 | 128 | 63 | 186 | 4.27E-73 | 227 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014303.01.T01 | Q9SA23 | 71.756 | 131 | 33 | 2 | 13 | 142 | 73 | 200 | 2.39E-58 | 183 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014303.01.T01 | B7FI84 | 90.323 | 124 | 12 | 0 | 5 | 128 | 63 | 186 | 2.04e-73 | 227 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380014303.01 | MsG0280008648.01 | PPI |
| MsG0580029609.01 | MsG0380014303.01 | PPI |
| MsG0380014303.01 | MsG0480022492.01 | PPI |
| MsG0380014303.01 | MsG0480022492.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014303.01.T01 | MTR_8g031190 | 90.323 | 124 | 12 | 0 | 5 | 128 | 63 | 186 | 2.25e-76 | 226 |
| MsG0380014303.01.T01 | MTR_3g055200 | 91.129 | 124 | 11 | 0 | 5 | 128 | 95 | 218 | 2.85e-76 | 227 |
| MsG0380014303.01.T01 | MTR_5g094010 | 82.090 | 67 | 12 | 0 | 13 | 79 | 73 | 139 | 6.24e-34 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014303.01.T01 | AT1G16240 | 70.992 | 131 | 37 | 1 | 13 | 142 | 73 | 203 | 2.02e-59 | 183 |
| MsG0380014303.01.T01 | AT1G16240 | 71.756 | 131 | 33 | 2 | 13 | 142 | 73 | 200 | 2.43e-59 | 183 |
| MsG0380014303.01.T01 | AT1G16240 | 71.756 | 131 | 33 | 2 | 13 | 142 | 73 | 200 | 2.43e-59 | 183 |
| MsG0380014303.01.T01 | AT1G16240 | 71.756 | 131 | 33 | 2 | 13 | 142 | 73 | 200 | 2.43e-59 | 183 |
| MsG0380014303.01.T01 | AT1G79590 | 75.214 | 117 | 28 | 1 | 13 | 128 | 74 | 190 | 4.17e-57 | 177 |
| MsG0380014303.01.T01 | AT1G79590 | 75.214 | 117 | 28 | 1 | 13 | 128 | 74 | 190 | 4.17e-57 | 177 |
| MsG0380014303.01.T01 | AT1G79590 | 75.214 | 117 | 28 | 1 | 13 | 128 | 105 | 221 | 5.27e-57 | 178 |
| MsG0380014303.01.T01 | AT1G16230 | 61.538 | 117 | 44 | 1 | 13 | 129 | 73 | 188 | 7.70e-42 | 137 |
| MsG0380014303.01.T01 | AT1G16225 | 52.212 | 113 | 51 | 1 | 13 | 125 | 49 | 158 | 1.99e-28 | 103 |
| MsG0380014303.01.T01 | AT1G16225 | 52.212 | 113 | 51 | 1 | 13 | 125 | 73 | 182 | 3.99e-28 | 103 |
| MsG0380014303.01.T01 | AT1G16225 | 49.254 | 67 | 31 | 1 | 13 | 79 | 73 | 136 | 6.86e-12 | 59.3 |
Find 31 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGCATCCGGTTTAATTTC+TGG | 0.251487 | 3:-52701825 | None:intergenic |
| AGATGAGGGCCTTGAGAAAT+TGG | 0.280110 | 3:+52701997 | MsG0380014303.01.T01:CDS |
| TTCTTTGTCAAATGATAAAT+AGG | 0.332707 | 3:+52701685 | MsG0380014303.01.T01:CDS |
| TGTTCAAAGTTGAAGCGATT+TGG | 0.364115 | 3:-52701767 | None:intergenic |
| CATGTCAAACTTTGCAAGTA+GGG | 0.424671 | 3:+52701788 | MsG0380014303.01.T01:CDS |
| TTGGATAACAATGGACTTGT+TGG | 0.440074 | 3:+52701864 | MsG0380014303.01.T01:CDS |
| CTCTTACCACAACTTTCTTT+AGG | 0.443448 | 3:+52702238 | MsG0380014303.01.T01:CDS |
| AATATGTTTCGTACTTAATA+CGG | 0.447438 | 3:-52702026 | None:intergenic |
| ACAGTTCTGCTCATTGCATC+CGG | 0.457368 | 3:-52701837 | None:intergenic |
| GCAAGTAGGGATAGTTTGCT+CGG | 0.468877 | 3:+52701801 | MsG0380014303.01.T01:CDS |
| AGGACAACCTTGCAAATTTG+AGG | 0.487031 | 3:+52701733 | MsG0380014303.01.T01:CDS |
| ATGTAGATGTCACAGATTCT+CGG | 0.487590 | 3:+52702203 | MsG0380014303.01.T01:CDS |
| CCTTGCAAATTTGAGGTCAA+AGG | 0.488877 | 3:+52701740 | MsG0380014303.01.T01:CDS |
| ACATGTCAAACTTTGCAAGT+AGG | 0.506274 | 3:+52701787 | MsG0380014303.01.T01:CDS |
| GCTCGGGCCAGAAATTAAAC+CGG | 0.520325 | 3:+52701818 | MsG0380014303.01.T01:CDS |
| ATGTCACAGATTCTCGGCCG+AGG | 0.549772 | 3:+52702209 | MsG0380014303.01.T01:CDS |
| CAAGTAGGGATAGTTTGCTC+GGG | 0.550936 | 3:+52701802 | MsG0380014303.01.T01:CDS |
| ATGGACTTGTTGGTCTGCAA+CGG | 0.564781 | 3:+52701874 | MsG0380014303.01.T01:CDS |
| CGGTCTCCTCCAATTTCTCA+AGG | 0.568939 | 3:-52702006 | None:intergenic |
| CCTTTGACCTCAAATTTGCA+AGG | 0.577028 | 3:-52701740 | None:intergenic |
| TTTCTAACAGAGCAAGATGA+GGG | 0.582199 | 3:+52701983 | MsG0380014303.01.T01:intron |
| AAGTACGAAACATATTTCGT+TGG | 0.587897 | 3:+52702033 | MsG0380014303.01.T01:CDS |
| CTGCAACGGCAAGTTATGAA+AGG | 0.592264 | 3:+52701888 | MsG0380014303.01.T01:CDS |
| TGAGGGCCTTGAGAAATTGG+AGG | 0.594430 | 3:+52702000 | MsG0380014303.01.T01:CDS |
| GATGCAATGAGCAGAACTGT+TGG | 0.599434 | 3:+52701840 | MsG0380014303.01.T01:CDS |
| TGTCACAGATTCTCGGCCGA+GGG | 0.605718 | 3:+52702210 | MsG0380014303.01.T01:CDS |
| AATGAGCAGAACTGTTGGCT+TGG | 0.609524 | 3:+52701845 | MsG0380014303.01.T01:CDS |
| ACTGTTGGCTTGGATAACAA+TGG | 0.640180 | 3:+52701855 | MsG0380014303.01.T01:CDS |
| TGTGGTAAGAGTGTTACCCT+CGG | 0.652117 | 3:-52702226 | None:intergenic |
| GAAAGAGATGAATCGTCGCA+AGG | 0.664446 | 3:+52701713 | MsG0380014303.01.T01:CDS |
| TATATGCCTAAAGAAAGTTG+TGG | 0.697010 | 3:-52702244 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATGTTTCGTACTTAATA+CGG | - | Chr3:52702029-52702048 | None:intergenic | 20.0% |
| !! | TTCTTTGTCAAATGATAAAT+AGG | + | Chr3:52701685-52701704 | MsG0380014303.01.T01:CDS | 20.0% |
| ! | AAAGACAATTAAGATAGGAT+GGG | - | Chr3:52702104-52702123 | None:intergenic | 25.0% |
| ! | TTAGCAAAGACAATTAAGAT+AGG | - | Chr3:52702109-52702128 | None:intergenic | 25.0% |
| !! | AAATACATAACTTGTGCTTT+GGG | + | Chr3:52701940-52701959 | MsG0380014303.01.T01:intron | 25.0% |
| AAGTACGAAACATATTTCGT+TGG | + | Chr3:52702033-52702052 | MsG0380014303.01.T01:CDS | 30.0% | |
| CAAAGACAATTAAGATAGGA+TGG | - | Chr3:52702105-52702124 | None:intergenic | 30.0% | |
| TATATGCCTAAAGAAAGTTG+TGG | - | Chr3:52702247-52702266 | None:intergenic | 30.0% | |
| ! | GAAATACATAACTTGTGCTT+TGG | + | Chr3:52701939-52701958 | MsG0380014303.01.T01:intron | 30.0% |
| ! | TTTTCAGACCGATAGAAAAA+CGG | - | Chr3:52702143-52702162 | None:intergenic | 30.0% |
| !! | TTTGAGATCCGTTTTTCTAT+CGG | + | Chr3:52702132-52702151 | MsG0380014303.01.T01:intron | 30.0% |
| !!! | GAATCTCTTTATTTTCTGCT+AGG | + | Chr3:52702165-52702184 | MsG0380014303.01.T01:intron | 30.0% |
| ACATGTCAAACTTTGCAAGT+AGG | + | Chr3:52701787-52701806 | MsG0380014303.01.T01:CDS | 35.0% | |
| ATGTAGATGTCACAGATTCT+CGG | + | Chr3:52702203-52702222 | MsG0380014303.01.T01:CDS | 35.0% | |
| CATGTCAAACTTTGCAAGTA+GGG | + | Chr3:52701788-52701807 | MsG0380014303.01.T01:CDS | 35.0% | |
| CTCTTACCACAACTTTCTTT+AGG | + | Chr3:52702238-52702257 | MsG0380014303.01.T01:CDS | 35.0% | |
| TGTTCAAAGTTGAAGCGATT+TGG | - | Chr3:52701770-52701789 | None:intergenic | 35.0% | |
| TTTCTAACAGAGCAAGATGA+GGG | + | Chr3:52701983-52702002 | MsG0380014303.01.T01:intron | 35.0% | |
| ! | ATTGCATCCGGTTTAATTTC+TGG | - | Chr3:52701828-52701847 | None:intergenic | 35.0% |
| ! | TATTTTCTGCTAGGATGACT+TGG | + | Chr3:52702174-52702193 | MsG0380014303.01.T01:intron | 35.0% |
| ! | TTGGATAACAATGGACTTGT+TGG | + | Chr3:52701864-52701883 | MsG0380014303.01.T01:CDS | 35.0% |
| ! | TTTTCTAACAGAGCAAGATG+AGG | + | Chr3:52701982-52702001 | MsG0380014303.01.T01:intron | 35.0% |
| AGGACAACCTTGCAAATTTG+AGG | + | Chr3:52701733-52701752 | MsG0380014303.01.T01:CDS | 40.0% | |
| CCTTTGACCTCAAATTTGCA+AGG | - | Chr3:52701743-52701762 | None:intergenic | 40.0% | |
| ! | CCTTGCAAATTTGAGGTCAA+AGG | + | Chr3:52701740-52701759 | MsG0380014303.01.T01:CDS | 40.0% |
| !! | ACTGTTGGCTTGGATAACAA+TGG | + | Chr3:52701855-52701874 | MsG0380014303.01.T01:CDS | 40.0% |
| !! | GCTTTGGGTATTGATTTCAG+AGG | + | Chr3:52701955-52701974 | MsG0380014303.01.T01:intron | 40.0% |
| ACAGTTCTGCTCATTGCATC+CGG | - | Chr3:52701840-52701859 | None:intergenic | 45.0% | |
| AGATGAGGGCCTTGAGAAAT+TGG | + | Chr3:52701997-52702016 | MsG0380014303.01.T01:CDS | 45.0% | |
| CTGCAACGGCAAGTTATGAA+AGG | + | Chr3:52701888-52701907 | MsG0380014303.01.T01:CDS | 45.0% | |
| GAAAGAGATGAATCGTCGCA+AGG | + | Chr3:52701713-52701732 | MsG0380014303.01.T01:CDS | 45.0% | |
| GATGCAATGAGCAGAACTGT+TGG | + | Chr3:52701840-52701859 | MsG0380014303.01.T01:CDS | 45.0% | |
| ! | AATGAGCAGAACTGTTGGCT+TGG | + | Chr3:52701845-52701864 | MsG0380014303.01.T01:CDS | 45.0% |
| !! | ATGGACTTGTTGGTCTGCAA+CGG | + | Chr3:52701874-52701893 | MsG0380014303.01.T01:CDS | 45.0% |
| !! | CAAGTAGGGATAGTTTGCTC+GGG | + | Chr3:52701802-52701821 | MsG0380014303.01.T01:CDS | 45.0% |
| !! | GCAAGTAGGGATAGTTTGCT+CGG | + | Chr3:52701801-52701820 | MsG0380014303.01.T01:CDS | 45.0% |
| !! | TGTGGTAAGAGTGTTACCCT+CGG | - | Chr3:52702229-52702248 | None:intergenic | 45.0% |
| CGGTCTCCTCCAATTTCTCA+AGG | - | Chr3:52702009-52702028 | None:intergenic | 50.0% | |
| GCTCGGGCCAGAAATTAAAC+CGG | + | Chr3:52701818-52701837 | MsG0380014303.01.T01:CDS | 50.0% | |
| TGAGGGCCTTGAGAAATTGG+AGG | + | Chr3:52702000-52702019 | MsG0380014303.01.T01:CDS | 50.0% | |
| ATGTCACAGATTCTCGGCCG+AGG | + | Chr3:52702209-52702228 | MsG0380014303.01.T01:CDS | 55.0% | |
| TGTCACAGATTCTCGGCCGA+GGG | + | Chr3:52702210-52702229 | MsG0380014303.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 52701672 | 52702276 | 52701672 | ID=MsG0380014303.01;Name=MsG0380014303.01 |
| Chr3 | mRNA | 52701672 | 52702276 | 52701672 | ID=MsG0380014303.01.T01;Parent=MsG0380014303.01;Name=MsG0380014303.01.T01;_AED=0.51;_eAED=0.52;_QI=0|1|0.33|1|1|1|3|0|142 |
| Chr3 | exon | 52701672 | 52701909 | 52701672 | ID=MsG0380014303.01.T01:exon:10443;Parent=MsG0380014303.01.T01 |
| Chr3 | exon | 52701993 | 52702093 | 52701993 | ID=MsG0380014303.01.T01:exon:10444;Parent=MsG0380014303.01.T01 |
| Chr3 | exon | 52702187 | 52702276 | 52702187 | ID=MsG0380014303.01.T01:exon:10445;Parent=MsG0380014303.01.T01 |
| Chr3 | CDS | 52701672 | 52701909 | 52701672 | ID=MsG0380014303.01.T01:cds;Parent=MsG0380014303.01.T01 |
| Chr3 | CDS | 52701993 | 52702093 | 52701993 | ID=MsG0380014303.01.T01:cds;Parent=MsG0380014303.01.T01 |
| Chr3 | CDS | 52702187 | 52702276 | 52702187 | ID=MsG0380014303.01.T01:cds;Parent=MsG0380014303.01.T01 |
| Gene Sequence |
| Protein sequence |
