AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029609.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029609.01.T01 MTR_5g089370 74.570 291 2 2 1 291 1 219 3.14e-148 414
MsG0580029609.01.T01 MTR_4g115390 47.458 295 79 3 1 291 1 223 4.90e-95 280
MsG0580029609.01.T01 MTR_4g052550 48.927 233 76 1 1 233 1 190 2.62e-79 239
MsG0580029609.01.T01 MTR_1g047720 28.014 282 133 5 3 282 6 219 2.53e-37 132
MsG0580029609.01.T01 MTR_4g022570 32.618 233 111 4 3 233 6 194 1.20e-36 130
MsG0580029609.01.T01 MTR_7g058900 32.759 232 112 4 3 233 6 194 4.58e-36 129
MsG0580029609.01.T01 MTR_1g111890 30.040 253 112 4 2 233 5 213 4.19e-35 127
MsG0580029609.01.T01 MTR_2g028790 32.328 232 113 4 3 233 10 198 9.93e-34 123
MsG0580029609.01.T01 MTR_4g083490 30.472 233 116 4 3 233 8 196 5.24e-33 121
MsG0580029609.01.T01 MTR_2g436870 30.172 232 116 4 4 233 7 194 1.01e-30 115
MsG0580029609.01.T01 MTR_7g058640 28.326 233 121 4 3 233 6 194 5.83e-27 105
MsG0580029609.01.T01 MTR_7g058640 28.704 216 108 4 2 215 5 176 7.13e-23 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029609.01.T01 AT5G11150 56.849 292 53 3 1 291 1 220 3.06e-119 341
MsG0580029609.01.T01 AT5G11150 56.849 292 53 3 1 291 1 220 7.34e-119 340
MsG0580029609.01.T01 AT4G32150 60.000 290 44 2 1 290 1 218 8.87e-114 327
MsG0580029609.01.T01 AT2G25340 58.621 290 48 2 1 290 1 218 2.71e-111 321
MsG0580029609.01.T01 AT5G22360 42.612 291 94 3 1 290 1 219 2.69e-82 247
MsG0580029609.01.T01 AT2G33120 32.189 233 112 4 3 233 6 194 2.35e-36 130
MsG0580029609.01.T01 AT1G04750 31.760 233 113 4 3 233 6 194 2.15e-35 127
MsG0580029609.01.T01 AT2G32670 32.759 232 112 4 3 233 71 259 6.23e-35 128
MsG0580029609.01.T01 AT1G04760 30.043 233 117 4 3 233 6 194 6.87e-35 126
MsG0580029609.01.T01 AT1G04760 30.043 233 117 4 3 233 6 194 6.87e-35 126
MsG0580029609.01.T01 AT3G54300 28.854 253 115 4 2 233 5 213 1.93e-34 125
MsG0580029609.01.T01 AT3G54300 28.854 253 115 4 2 233 5 213 1.93e-34 125
MsG0580029609.01.T01 AT3G54300 28.854 253 115 4 2 233 5 213 1.93e-34 125
MsG0580029609.01.T01 AT2G33120 30.705 241 113 5 3 233 6 202 3.12e-33 122
MsG0580029609.01.T01 AT4G15780 29.956 227 109 5 4 229 7 184 2.53e-27 105
MsG0580029609.01.T01 AT2G33110 30.901 233 111 8 3 233 6 190 1.65e-23 95.9
MsG0580029609.01.T01 AT2G33110 32.000 200 92 5 3 201 6 162 1.02e-21 90.5
MsG0580029609.01.T01 AT2G33120 35.065 77 50 0 157 233 36 112 3.69e-11 60.5

Find 79 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGCCTTGCAACAAATTTC+TGG 0.200994 5:+99358607 MsG0580029609.01.T01:three_prime_UTR
CCGTTATTCACGATTCAAAA+TGG 0.202220 5:+99355509 MsG0580029609.01.T01:exon
GCAACAAATTTCTGGTGTTT+TGG 0.204706 5:+99358615 MsG0580029609.01.T01:three_prime_UTR
AGAAAATATTGACAAAGTTT+TGG 0.239741 5:+99356745 MsG0580029609.01.T01:CDS
AACATGTGTATCGTTGTTTC+CGG 0.248239 5:-99355637 None:intergenic
GATAGGATTAATCGGCTTAA+AGG 0.299245 5:+99355900 MsG0580029609.01.T01:CDS
GTTTGGACTGACAGTGAATT+TGG 0.321615 5:-99356221 None:intergenic
CAGAGGTTTGTAAGGAGTTA+TGG 0.338003 5:+99355780 MsG0580029609.01.T01:CDS
CAAACATATGTGTGCGTGTT+TGG 0.342286 5:+99356239 MsG0580029609.01.T01:CDS
AAAGTGTTGTTATAGCTTGA+TGG 0.353719 5:+99358765 MsG0580029609.01.T01:three_prime_UTR
GTCAAGATTATGATGGCTTA+TGG 0.359479 5:+99356190 MsG0580029609.01.T01:intron
GCAATTGAAATCAAAACAAA+TGG 0.381148 5:-99358556 None:intergenic
GTGTCCTTTCATATCATCTC+TGG 0.400774 5:+99358488 MsG0580029609.01.T01:three_prime_UTR
AGATTCATCAGAGGTTTGTA+AGG 0.404505 5:+99355772 MsG0580029609.01.T01:CDS
AGGTGATAGATTGGAATTGC+TGG 0.413308 5:+99356772 MsG0580029609.01.T01:CDS
TTTGGATAGAGGTGATAGAT+TGG 0.413502 5:+99356763 MsG0580029609.01.T01:CDS
CAAACACGCACACATATGTT+TGG 0.417062 5:-99356238 None:intergenic
CGCGCTGGTGGCGCGTGGAA+CGG 0.419354 5:+99355542 MsG0580029609.01.T01:CDS
ACATGTGTATCGTTGTTTCC+GGG 0.436400 5:-99355636 None:intergenic
GGCGGCGTTTGTTGTTGTTC+CGG 0.443043 5:-99355583 None:intergenic
CGTTATTCACGATTCAAAAT+GGG 0.447571 5:+99355510 MsG0580029609.01.T01:exon
CCTATCAGCATTCGGATCAC+TGG 0.457235 5:-99355883 None:intergenic
CGAATGCTGATAGGATTAAT+CGG 0.459876 5:+99355892 MsG0580029609.01.T01:CDS
AGAACTGGAACAAACATCAT+TGG 0.465201 5:+99358727 MsG0580029609.01.T01:three_prime_UTR
ACCGTCTTGCTTCTAGTGAA+TGG 0.468398 5:+99358450 MsG0580029609.01.T01:exon
CCAGTGATCCGAATGCTGAT+AGG 0.476298 5:+99355883 MsG0580029609.01.T01:CDS
TGGACTCACGGTGCTGTGTA+TGG 0.483242 5:+99355704 MsG0580029609.01.T01:CDS
ACTATCACCCTTGTAGATGC+AGG 0.483563 5:+99357626 MsG0580029609.01.T01:CDS
GAACTGGAACAAACATCATT+GGG 0.485467 5:+99358728 MsG0580029609.01.T01:three_prime_UTR
CAAACGCCGCCGCAATCGCG+CGG 0.490314 5:+99355595 MsG0580029609.01.T01:CDS
CGATTAATCCTATCAGCATT+CGG 0.491371 5:-99355891 None:intergenic
TGACCGCATTGAATTGGTCA+TGG 0.497335 5:-99357601 None:intergenic
CTTCTATTTATCTTCAATTG+TGG 0.501020 5:+99358524 MsG0580029609.01.T01:three_prime_UTR
GTCGCTTTAGAAGCACTGTC+TGG 0.502008 5:+99356849 MsG0580029609.01.T01:CDS
CATGTGTATCGTTGTTTCCG+GGG 0.512854 5:-99355635 None:intergenic
TGTGATAGCATATGTTGTGT+TGG 0.514806 5:+99358402 MsG0580029609.01.T01:CDS
TAGAAGCAAGACGGTAGTGC+AGG 0.518587 5:-99358442 None:intergenic
GACCGCATTGAATTGGTCAT+GGG 0.522553 5:-99357600 None:intergenic
CTCTACGCGCTGGTGGCGCG+TGG 0.524194 5:+99355537 MsG0580029609.01.T01:CDS
AACACCAGAAATTTGTTGCA+AGG 0.525105 5:-99358611 None:intergenic
GCTTAAAGGTGAAATGTCTC+AGG 0.529462 5:+99355914 MsG0580029609.01.T01:CDS
AGGAATACCCTGCATCTACA+AGG 0.530963 5:-99357634 None:intergenic
ATCTCTTCAAGAAACGCAAA+CGG 0.535896 5:-99355753 None:intergenic
GAGGATTTGCCGCGCGATTG+CGG 0.538753 5:-99355604 None:intergenic
GTGGTTCTCGCGGAATTCAC+CGG 0.548241 5:+99355564 MsG0580029609.01.T01:CDS
CAAATCCTCGAGAAAATCCC+CGG 0.556101 5:+99355618 MsG0580029609.01.T01:CDS
TTAGAAGCACTGTCTGGTGG+AGG 0.558410 5:+99356855 MsG0580029609.01.T01:CDS
CAATTGAAATCAAAACAAAT+GGG 0.562866 5:-99358555 None:intergenic
ACCTTTCAATGAGCTACGAC+GGG 0.566127 5:-99358844 None:intergenic
TTATAGCTTGATGGAGTCAT+CGG 0.567492 5:+99358774 MsG0580029609.01.T01:three_prime_UTR
ATTTATCTTCAATTGTGGCG+TGG 0.571192 5:+99358529 MsG0580029609.01.T01:three_prime_UTR
GCTGGTGGCGCGTGGAACGG+TGG 0.578906 5:+99355545 MsG0580029609.01.T01:CDS
GATAAAGCTGCTAATATGCA+AGG 0.579433 5:+99356797 MsG0580029609.01.T01:CDS
GCGTGGAACGGTGGTTCTCG+CGG 0.582843 5:+99355554 MsG0580029609.01.T01:CDS
ATGTTTCGTATTCACAAGAT+CGG 0.583230 5:+99355655 MsG0580029609.01.T01:CDS
CAACATATGCTATCACAAGT+AGG 0.588389 5:-99358396 None:intergenic
GATTTGCCGCGCGATTGCGG+CGG 0.588839 5:-99355601 None:intergenic
GCTTTAGAAGCACTGTCTGG+TGG 0.589136 5:+99356852 MsG0580029609.01.T01:CDS
AGTCAAAAGTTATTCTGTAG+TGG 0.590154 5:+99356277 MsG0580029609.01.T01:CDS
TGATAGATTGGAATTGCTGG+TGG 0.593278 5:+99356775 MsG0580029609.01.T01:CDS
AAGACGGTAGTGCAGGCCCA+TGG 0.602690 5:-99358435 None:intergenic
CTATCACCCTTGTAGATGCA+GGG 0.604825 5:+99357627 MsG0580029609.01.T01:CDS
TCCCGTCGTAGCTCATTGAA+AGG 0.606260 5:+99358843 MsG0580029609.01.T01:three_prime_UTR
GAAACGAACCGATGGACTCA+CGG 0.607839 5:+99355692 MsG0580029609.01.T01:CDS
CAAGCCAGAGATGATATGAA+AGG 0.608146 5:-99358492 None:intergenic
ACACAGCACCGTGAGTCCAT+CGG 0.624165 5:-99355700 None:intergenic
GACCTTTCAATGAGCTACGA+CGG 0.624182 5:-99358845 None:intergenic
TTTCACGTGAAACGAACCGA+TGG 0.628828 5:+99355684 MsG0580029609.01.T01:CDS
GGGTATACTCTACGCGCTGG+TGG 0.634486 5:+99355530 MsG0580029609.01.T01:CDS
AATGGGTATACTCTACGCGC+TGG 0.637918 5:+99355527 MsG0580029609.01.T01:exon
TGTAAGGAGTTATGGACGTG+CGG 0.656982 5:+99355788 MsG0580029609.01.T01:CDS
TTCTTGAAGAGATTCATCAG+AGG 0.658130 5:+99355763 MsG0580029609.01.T01:CDS
GGAATACCCTGCATCTACAA+GGG 0.660746 5:-99357633 None:intergenic
ACCATTCACTAGAAGCAAGA+CGG 0.663304 5:-99358451 None:intergenic
TGGCGGATGATAGCGTTGGA+CGG 0.663803 5:+99355724 MsG0580029609.01.T01:CDS
TGTATGGCGGATGATAGCGT+TGG 0.668509 5:+99355720 MsG0580029609.01.T01:CDS
TCACAATTGTGCCCGCAATG+CGG 0.703949 5:+99357562 MsG0580029609.01.T01:CDS
ACTCACGGTGCTGTGTATGG+CGG 0.734585 5:+99355707 MsG0580029609.01.T01:CDS
ATCCCATGACCAATTCAATG+CGG 0.756371 5:+99357598 MsG0580029609.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAAAAAAAAACTTATAAA+CGG - Chr5:99356957-99356976 None:intergenic 10.0%
!! TAAATAATTCAAAAAGAAAA+TGG - Chr5:99358804-99358823 None:intergenic 10.0%
!!! TTCTATTTTTTTTTTTTTAC+AGG + Chr5:99356706-99356725 MsG0580029609.01.T01:intron 10.0%
!!! TTTTATTTCTTACAAATAAA+AGG - Chr5:99358172-99358191 None:intergenic 10.0%
!! AAGAAATTAAAATATTGTTG+TGG - Chr5:99357691-99357710 None:intergenic 15.0%
!!! AAATTGATTTTGATATGTTT+GGG + Chr5:99356302-99356321 MsG0580029609.01.T01:CDS 15.0%
!!! GTGTTATCTTTTTTTTTTTT+TGG - Chr5:99355360-99355379 None:intergenic 15.0%
!! ATAATATAACCCCAAAAAAT+TGG + Chr5:99358702-99358721 MsG0580029609.01.T01:three_prime_UTR 20.0%
!! CAATTGAAATCAAAACAAAT+GGG - Chr5:99358558-99358577 None:intergenic 20.0%
!! GAATAAAATATACAACACTT+TGG - Chr5:99357788-99357807 None:intergenic 20.0%
!! GACAAATATTCTATCTTTAT+AGG - Chr5:99357002-99357021 None:intergenic 20.0%
!! GTTGAAAATTTGAATTTACA+CGG + Chr5:99356018-99356037 MsG0580029609.01.T01:intron 20.0%
!!! AAAATCAATTTTAAATCTGC+AGG - Chr5:99356410-99356429 None:intergenic 20.0%
!!! AATTGATTTAAGTTGTTTGT+AGG - Chr5:99356598-99356617 None:intergenic 20.0%
!!! AATTGATTTTGATATGTTTG+GGG + Chr5:99356303-99356322 MsG0580029609.01.T01:CDS 20.0%
!!! AATTTTGAAATTTCATGTCA+TGG + Chr5:99358234-99358253 MsG0580029609.01.T01:intron 20.0%
!!! AGAAAATATTGACAAAGTTT+TGG + Chr5:99356745-99356764 MsG0580029609.01.T01:CDS 20.0%
!!! GAAATTGATTTTGATATGTT+TGG + Chr5:99356301-99356320 MsG0580029609.01.T01:CDS 20.0%
!!! TATGAATGATGAATTTTCTA+GGG + Chr5:99355833-99355852 MsG0580029609.01.T01:CDS 20.0%
!!! TCTGTTTTAATTATCATTCT+TGG + Chr5:99357062-99357081 MsG0580029609.01.T01:intron 20.0%
! AAAAAACATCAGTAGAATCA+AGG + Chr5:99358055-99358074 MsG0580029609.01.T01:intron 25.0%
! AAGATAGAATATTTGTCTCT+TGG + Chr5:99357005-99357024 MsG0580029609.01.T01:intron 25.0%
! AATTAAAACAGACGAAGAAA+AGG - Chr5:99357054-99357073 None:intergenic 25.0%
! AATTAAGTGTATGTTTCGAT+TGG + Chr5:99356099-99356118 MsG0580029609.01.T01:intron 25.0%
! AGATAGAATATTTGTCTCTT+GGG + Chr5:99357006-99357025 MsG0580029609.01.T01:intron 25.0%
! ATCACATTTATTAATCTCTG+AGG - Chr5:99357147-99357166 None:intergenic 25.0%
! ATGATACATTGAATTAGAAG+TGG + Chr5:99355992-99356011 MsG0580029609.01.T01:intron 25.0%
! CTTCTATTTATCTTCAATTG+TGG + Chr5:99358524-99358543 MsG0580029609.01.T01:three_prime_UTR 25.0%
! CTTTATAGGTTCAACTTATA+TGG - Chr5:99356988-99357007 None:intergenic 25.0%
! GAATAATGTTTGTCAAACTT+AGG + Chr5:99356654-99356673 MsG0580029609.01.T01:intron 25.0%
! GCAATTGAAATCAAAACAAA+TGG - Chr5:99358559-99358578 None:intergenic 25.0%
! TCACATTTATTAATCTCTGA+GGG - Chr5:99357146-99357165 None:intergenic 25.0%
! TGATACATTGAATTAGAAGT+GGG + Chr5:99355993-99356012 MsG0580029609.01.T01:intron 25.0%
! TTCTACTGGGAAAAAAAATT+CGG - Chr5:99357313-99357332 None:intergenic 25.0%
!! ATAGGATTTGTCAATTTTCA+TGG + Chr5:99357093-99357112 MsG0580029609.01.T01:intron 25.0%
!! CTATGAATGATGAATTTTCT+AGG + Chr5:99355832-99355851 MsG0580029609.01.T01:CDS 25.0%
!! TAGTTATGTTCTTCTTTTTG+TGG + Chr5:99355940-99355959 MsG0580029609.01.T01:intron 25.0%
!! TCATTTTATCGTTTATCTTC+CGG - Chr5:99357457-99357476 None:intergenic 25.0%
!!! TTGAGATTTTGTTTTTGGAA+AGG - Chr5:99357657-99357676 None:intergenic 25.0%
AGTCAAAAGTTATTCTGTAG+TGG + Chr5:99356277-99356296 MsG0580029609.01.T01:CDS 30.0%
ATGTTTCGTATTCACAAGAT+CGG + Chr5:99355655-99355674 MsG0580029609.01.T01:CDS 30.0%
CGTTATTCACGATTCAAAAT+GGG + Chr5:99355510-99355529 MsG0580029609.01.T01:exon 30.0%
GTTGTTTGTAGGTTTATGTT+TGG - Chr5:99356587-99356606 None:intergenic 30.0%
TAATTGAATTCTTACCAGAC+AGG - Chr5:99358210-99358229 None:intergenic 30.0%
TCGATGTAAAAAATGCGTTT+AGG - Chr5:99356529-99356548 None:intergenic 30.0%
TGAAATTGAGACAATGAACT+AGG + Chr5:99357739-99357758 MsG0580029609.01.T01:intron 30.0%
TTCCATACAGTTTCAAATTG+CGG + Chr5:99357482-99357501 MsG0580029609.01.T01:intron 30.0%
TTGAAACTTAGGTTTACACA+TGG + Chr5:99357852-99357871 MsG0580029609.01.T01:intron 30.0%
! AACAGCAAAATAGTTCTACT+GGG - Chr5:99357326-99357345 None:intergenic 30.0%
! AGCTTTTGTCAAGATTATGA+TGG + Chr5:99356183-99356202 MsG0580029609.01.T01:intron 30.0%
! CCATTTTGAATCGTGAATAA+CGG - Chr5:99355512-99355531 None:intergenic 30.0%
! GTAATTTTTCGTTGCATATC+TGG + Chr5:99357398-99357417 MsG0580029609.01.T01:intron 30.0%
! TAACAGCAAAATAGTTCTAC+TGG - Chr5:99357327-99357346 None:intergenic 30.0%
! TCAGCACATTGTTTGTTAAA+GGG - Chr5:99357434-99357453 None:intergenic 30.0%
! TTTTCATTACTGAGGTTTGA+AGG + Chr5:99358645-99358664 MsG0580029609.01.T01:three_prime_UTR 30.0%
!! AAAGTGTTGTTATAGCTTGA+TGG + Chr5:99358765-99358784 MsG0580029609.01.T01:three_prime_UTR 30.0%
!! AGAGCTTTAGAATTCCAAAT+TGG + Chr5:99357970-99357989 MsG0580029609.01.T01:intron 30.0%
!! GAATTCTAAAGCTCTTTCTA+AGG - Chr5:99357965-99357984 None:intergenic 30.0%
!!! ATGGTGATTGTTTTTAGTAG+TGG + Chr5:99358253-99358272 MsG0580029609.01.T01:intron 30.0%
!!! ATTGACAAAGTTTTGGATAG+AGG + Chr5:99356752-99356771 MsG0580029609.01.T01:CDS 30.0%
!!! CAACAAATTTCTGGTGTTTT+GGG + Chr5:99358616-99358635 MsG0580029609.01.T01:three_prime_UTR 30.0%
!!! TTTTGATTTCAATTGCTGCT+GGG + Chr5:99358563-99358582 MsG0580029609.01.T01:three_prime_UTR 30.0%
AAACTGAAAGCAAGATTTGC+TGG + Chr5:99358032-99358051 MsG0580029609.01.T01:intron 35.0%
AAAGCCTTGCAACAAATTTC+TGG + Chr5:99358607-99358626 MsG0580029609.01.T01:three_prime_UTR 35.0%
AAGTCAGAATCAAACTCTAG+AGG - Chr5:99356483-99356502 None:intergenic 35.0%
AATATTGTTGTGGCAATGTG+CGG - Chr5:99357681-99357700 None:intergenic 35.0%
AGAACTGGAACAAACATCAT+TGG + Chr5:99358727-99358746 MsG0580029609.01.T01:three_prime_UTR 35.0%
AGATTCATCAGAGGTTTGTA+AGG + Chr5:99355772-99355791 MsG0580029609.01.T01:CDS 35.0%
AGTGTAATAACCATGATTGC+AGG - Chr5:99356377-99356396 None:intergenic 35.0%
ATCTCTTCAAGAAACGCAAA+CGG - Chr5:99355756-99355775 None:intergenic 35.0%
ATTTATCTTCAATTGTGGCG+TGG + Chr5:99358529-99358548 MsG0580029609.01.T01:three_prime_UTR 35.0%
CAACATATGCTATCACAAGT+AGG - Chr5:99358399-99358418 None:intergenic 35.0%
CCGTTATTCACGATTCAAAA+TGG + Chr5:99355509-99355528 MsG0580029609.01.T01:exon 35.0%
CGAATGCTGATAGGATTAAT+CGG + Chr5:99355892-99355911 MsG0580029609.01.T01:CDS 35.0%
CGATTAATCCTATCAGCATT+CGG - Chr5:99355894-99355913 None:intergenic 35.0%
CTCTTTGTAGGAGAATTCAA+AGG + Chr5:99358009-99358028 MsG0580029609.01.T01:intron 35.0%
GAACTGGAACAAACATCATT+GGG + Chr5:99358728-99358747 MsG0580029609.01.T01:three_prime_UTR 35.0%
GATAAAGCTGCTAATATGCA+AGG + Chr5:99356797-99356816 MsG0580029609.01.T01:CDS 35.0%
GATCGATGAATTGTAATCTG+AGG - Chr5:99358325-99358344 None:intergenic 35.0%
GTCAAGATTATGATGGCTTA+TGG + Chr5:99356190-99356209 MsG0580029609.01.T01:intron 35.0%
TCAACAAAATCACGTTTCCA+CGG - Chr5:99356145-99356164 None:intergenic 35.0%
TCATTCTTGGCATGATCAAT+AGG + Chr5:99357075-99357094 MsG0580029609.01.T01:intron 35.0%
TCCATACAGTTTCAAATTGC+GGG + Chr5:99357483-99357502 MsG0580029609.01.T01:intron 35.0%
TGTGATAGCATATGTTGTGT+TGG + Chr5:99358402-99358421 MsG0580029609.01.T01:CDS 35.0%
TTATAGCTTGATGGAGTCAT+CGG + Chr5:99358774-99358793 MsG0580029609.01.T01:three_prime_UTR 35.0%
TTCTTGAAGAGATTCATCAG+AGG + Chr5:99355763-99355782 MsG0580029609.01.T01:CDS 35.0%
TTGTCTCAATTTCAACACAC+TGG - Chr5:99357733-99357752 None:intergenic 35.0%
TTTGGATAGAGGTGATAGAT+TGG + Chr5:99356763-99356782 MsG0580029609.01.T01:CDS 35.0%
! AACATGTGTATCGTTGTTTC+CGG - Chr5:99355640-99355659 None:intergenic 35.0%
! GATAGGATTAATCGGCTTAA+AGG + Chr5:99355900-99355919 MsG0580029609.01.T01:CDS 35.0%
! GGTGGTATTTTTCATTACTG+AGG + Chr5:99358637-99358656 MsG0580029609.01.T01:three_prime_UTR 35.0%
! GTCAGCACATTGTTTGTTAA+AGG - Chr5:99357435-99357454 None:intergenic 35.0%
! TCATATTTTTCACCGCATTG+CGG - Chr5:99357577-99357596 None:intergenic 35.0%
! TGCGTGTTTGGATTCATTTT+TGG + Chr5:99356251-99356270 MsG0580029609.01.T01:CDS 35.0%
! TGCTGCAAAGTTTGAAACTT+AGG + Chr5:99357841-99357860 MsG0580029609.01.T01:intron 35.0%
! TTAACAAACAATGTGCTGAC+CGG + Chr5:99357435-99357454 MsG0580029609.01.T01:intron 35.0%
!! AACACCAGAAATTTGTTGCA+AGG - Chr5:99358614-99358633 None:intergenic 35.0%
!! GCGTGTTTGGATTCATTTTT+GGG + Chr5:99356252-99356271 MsG0580029609.01.T01:CDS 35.0%
!!! CAGTTCTCTCCAATTTTTTG+GGG - Chr5:99358714-99358733 None:intergenic 35.0%
!!! CCAGTTCTCTCCAATTTTTT+GGG - Chr5:99358715-99358734 None:intergenic 35.0%
!!! GCAACAAATTTCTGGTGTTT+TGG + Chr5:99358615-99358634 MsG0580029609.01.T01:three_prime_UTR 35.0%
!!! GCGGTTTGAGATTTTGTTTT+TGG - Chr5:99357662-99357681 None:intergenic 35.0%
!!! GTTTTGATTTCAATTGCTGC+TGG + Chr5:99358562-99358581 MsG0580029609.01.T01:three_prime_UTR 35.0%
!!! TAGTTTTGACCGCATTGAAT+TGG - Chr5:99357610-99357629 None:intergenic 35.0%
!!! TCCAGTTCTCTCCAATTTTT+TGG - Chr5:99358716-99358735 None:intergenic 35.0%
!!! TTTCAACACACTGGTTTTTG+AGG - Chr5:99357724-99357743 None:intergenic 35.0%
AACTAGTCAGAGTTAGAGAG+AGG + Chr5:99357372-99357391 MsG0580029609.01.T01:intron 40.0%
ACCATTCACTAGAAGCAAGA+CGG - Chr5:99358454-99358473 None:intergenic 40.0%
ACTAGTCAGAGTTAGAGAGA+GGG + Chr5:99357373-99357392 MsG0580029609.01.T01:intron 40.0%
ACTGTCTTCAGCTTAAGCTT+AGG + Chr5:99357230-99357249 MsG0580029609.01.T01:intron 40.0%
AGGTGATAGATTGGAATTGC+TGG + Chr5:99356772-99356791 MsG0580029609.01.T01:CDS 40.0%
ATCCCATGACCAATTCAATG+CGG + Chr5:99357598-99357617 MsG0580029609.01.T01:CDS 40.0%
ATTCCAAATTGGCGTATCCA+AGG + Chr5:99357981-99358000 MsG0580029609.01.T01:intron 40.0%
CAAACACGCACACATATGTT+TGG - Chr5:99356241-99356260 None:intergenic 40.0%
CAAACATATGTGTGCGTGTT+TGG + Chr5:99356239-99356258 MsG0580029609.01.T01:CDS 40.0%
CAAGCCAGAGATGATATGAA+AGG - Chr5:99358495-99358514 None:intergenic 40.0%
CAGAGGTTTGTAAGGAGTTA+TGG + Chr5:99355780-99355799 MsG0580029609.01.T01:CDS 40.0%
CCCAAAAAATTGGAGAGAAC+TGG + Chr5:99358712-99358731 MsG0580029609.01.T01:three_prime_UTR 40.0%
GCTTAAAGGTGAAATGTCTC+AGG + Chr5:99355914-99355933 MsG0580029609.01.T01:CDS 40.0%
GTGTCCTTTCATATCATCTC+TGG + Chr5:99358488-99358507 MsG0580029609.01.T01:three_prime_UTR 40.0%
GTTTGGACTGACAGTGAATT+TGG - Chr5:99356224-99356243 None:intergenic 40.0%
TATAAGTGCAATCCTGCACA+TGG - Chr5:99358371-99358390 None:intergenic 40.0%
TCCAAGGCAATTCTCTTTGT+AGG + Chr5:99357997-99358016 MsG0580029609.01.T01:intron 40.0%
TCCTACAAAGAGAATTGCCT+TGG - Chr5:99358001-99358020 None:intergenic 40.0%
TGATAGATTGGAATTGCTGG+TGG + Chr5:99356775-99356794 MsG0580029609.01.T01:CDS 40.0%
! ACATGTGTATCGTTGTTTCC+GGG - Chr5:99355639-99355658 None:intergenic 40.0%
! ACCCGCAATTTGAAACTGTA+TGG - Chr5:99357487-99357506 None:intergenic 40.0%
! ATTAATCTCTGAGGGTTCGA+AGG - Chr5:99357138-99357157 None:intergenic 40.0%
! CATATTTTTCACCGCATTGC+GGG - Chr5:99357576-99357595 None:intergenic 40.0%
!!! CAAATTTCTGGTGTTTTGGG+TGG + Chr5:99358619-99358638 MsG0580029609.01.T01:three_prime_UTR 40.0%
!!! TAGGGTTTTGAATCAGCAGA+TGG + Chr5:99355851-99355870 MsG0580029609.01.T01:CDS 40.0%
!!! TGAGTTTTGAGAAATCACCG+TGG + Chr5:99356125-99356144 MsG0580029609.01.T01:intron 40.0%
!!! TGTTGGCTTTTGTTTGCCAT+GGG + Chr5:99358419-99358438 MsG0580029609.01.T01:CDS 40.0%
ACCGTCTTGCTTCTAGTGAA+TGG + Chr5:99358450-99358469 MsG0580029609.01.T01:exon 45.0%
ACCTTTCAATGAGCTACGAC+GGG - Chr5:99358847-99358866 None:intergenic 45.0%
ACTATCACCCTTGTAGATGC+AGG + Chr5:99357626-99357645 MsG0580029609.01.T01:CDS 45.0%
AGGAATACCCTGCATCTACA+AGG - Chr5:99357637-99357656 None:intergenic 45.0%
CAAACTTAGGAATGTGCCAG+TGG + Chr5:99356667-99356686 MsG0580029609.01.T01:intron 45.0%
CAAATCCTCGAGAAAATCCC+CGG + Chr5:99355618-99355637 MsG0580029609.01.T01:CDS 45.0%
CTATCACCCTTGTAGATGCA+GGG + Chr5:99357627-99357646 MsG0580029609.01.T01:CDS 45.0%
GACAAAATGCTTCCATGTGC+AGG + Chr5:99358356-99358375 MsG0580029609.01.T01:intron 45.0%
GACCGCATTGAATTGGTCAT+GGG - Chr5:99357603-99357622 None:intergenic 45.0%
GACCTTTCAATGAGCTACGA+CGG - Chr5:99358848-99358867 None:intergenic 45.0%
GGAATACCCTGCATCTACAA+GGG - Chr5:99357636-99357655 None:intergenic 45.0%
GTCTTCAGCTTAAGCTTAGG+TGG + Chr5:99357233-99357252 MsG0580029609.01.T01:intron 45.0%
TCAGCTTAAGCTTAGGTGGT+GGG + Chr5:99357237-99357256 MsG0580029609.01.T01:intron 45.0%
TGACCGCATTGAATTGGTCA+TGG - Chr5:99357604-99357623 None:intergenic 45.0%
TGTAAGGAGTTATGGACGTG+CGG + Chr5:99355788-99355807 MsG0580029609.01.T01:CDS 45.0%
TTAAAGCAAGTGTGCCTGTC+TGG + Chr5:99358193-99358212 MsG0580029609.01.T01:intron 45.0%
TTCAGCTTAAGCTTAGGTGG+TGG + Chr5:99357236-99357255 MsG0580029609.01.T01:intron 45.0%
TTGCCTTGGATACGCCAATT+TGG - Chr5:99357987-99358006 None:intergenic 45.0%
TTTACACATGGCACACACGA+GGG + Chr5:99357864-99357883 MsG0580029609.01.T01:intron 45.0%
TTTCACGTGAAACGAACCGA+TGG + Chr5:99355684-99355703 MsG0580029609.01.T01:CDS 45.0%
! CATGTGTATCGTTGTTTCCG+GGG - Chr5:99355638-99355657 None:intergenic 45.0%
! GTAGCTTTTGCCTGCAATCA+TGG + Chr5:99356364-99356383 MsG0580029609.01.T01:intron 45.0%
!! GGTCGAAGCTTTTGTTTCGA+CGG - Chr5:99357944-99357963 None:intergenic 45.0%
!!! GTGTTGGCTTTTGTTTGCCA+TGG + Chr5:99358418-99358437 MsG0580029609.01.T01:CDS 45.0%
AATGGGTATACTCTACGCGC+TGG + Chr5:99355527-99355546 MsG0580029609.01.T01:exon 50.0%
ACCTGAGCAAAGACAACCAC+TGG - Chr5:99356686-99356705 None:intergenic 50.0%
CAGCTTAAGCTTAGGTGGTG+GGG + Chr5:99357238-99357257 MsG0580029609.01.T01:intron 50.0%
CAGTTTCAAATTGCGGGTCG+TGG + Chr5:99357489-99357508 MsG0580029609.01.T01:intron 50.0%
CCAGTGATCCGAATGCTGAT+AGG + Chr5:99355883-99355902 MsG0580029609.01.T01:CDS 50.0%
CCTATCAGCATTCGGATCAC+TGG - Chr5:99355886-99355905 None:intergenic 50.0%
GGGGATTGAGTAGAGGTTCT+AGG + Chr5:99357257-99357276 MsG0580029609.01.T01:intron 50.0%
GTTTACACATGGCACACACG+AGG + Chr5:99357863-99357882 MsG0580029609.01.T01:intron 50.0%
TAGAAGCAAGACGGTAGTGC+AGG - Chr5:99358445-99358464 None:intergenic 50.0%
TCACAATTGTGCCCGCAATG+CGG + Chr5:99357562-99357581 MsG0580029609.01.T01:CDS 50.0%
TCCCGTCGTAGCTCATTGAA+AGG + Chr5:99358843-99358862 MsG0580029609.01.T01:three_prime_UTR 50.0%
! GAAACGAACCGATGGACTCA+CGG + Chr5:99355692-99355711 MsG0580029609.01.T01:CDS 50.0%
! TGTTTCCGGGGATTTTCTCG+AGG - Chr5:99355626-99355645 None:intergenic 50.0%
!! GCTTTAGAAGCACTGTCTGG+TGG + Chr5:99356852-99356871 MsG0580029609.01.T01:CDS 50.0%
!! GTCGCTTTAGAAGCACTGTC+TGG + Chr5:99356849-99356868 MsG0580029609.01.T01:CDS 50.0%
!! TGTATGGCGGATGATAGCGT+TGG + Chr5:99355720-99355739 MsG0580029609.01.T01:CDS 50.0%
!! TTAGAAGCACTGTCTGGTGG+AGG + Chr5:99356855-99356874 MsG0580029609.01.T01:CDS 50.0%
ACACAGCACCGTGAGTCCAT+CGG - Chr5:99355703-99355722 None:intergenic 55.0%
GCCAGTGGTTGTCTTTGCTC+AGG + Chr5:99356682-99356701 MsG0580029609.01.T01:intron 55.0%
GTGGTTCTCGCGGAATTCAC+CGG + Chr5:99355564-99355583 MsG0580029609.01.T01:CDS 55.0%
! TGGCGGATGATAGCGTTGGA+CGG + Chr5:99355724-99355743 MsG0580029609.01.T01:CDS 55.0%
!! ACTCACGGTGCTGTGTATGG+CGG + Chr5:99355707-99355726 MsG0580029609.01.T01:CDS 55.0%
!! AGGTGGTGGGGATTGAGTAG+AGG + Chr5:99357250-99357269 MsG0580029609.01.T01:intron 55.0%
!! GGCGGCGTTTGTTGTTGTTC+CGG - Chr5:99355586-99355605 None:intergenic 55.0%
!! TGGACTCACGGTGCTGTGTA+TGG + Chr5:99355704-99355723 MsG0580029609.01.T01:CDS 55.0%
!!! TGGACGTGCGGTTTTGACTG+CGG + Chr5:99355800-99355819 MsG0580029609.01.T01:CDS 55.0%
AAGACGGTAGTGCAGGCCCA+TGG - Chr5:99358438-99358457 None:intergenic 60.0%
CTTGGGTCGAGCGAAGCTGT+TGG + Chr5:99357023-99357042 MsG0580029609.01.T01:intron 60.0%
GAGGATTTGCCGCGCGATTG+CGG - Chr5:99355607-99355626 None:intergenic 60.0%
GGGTATACTCTACGCGCTGG+TGG + Chr5:99355530-99355549 MsG0580029609.01.T01:CDS 60.0%
GATTTGCCGCGCGATTGCGG+CGG - Chr5:99355604-99355623 None:intergenic 65.0%
GCGTGGAACGGTGGTTCTCG+CGG + Chr5:99355554-99355573 MsG0580029609.01.T01:CDS 65.0%
CAAACGCCGCCGCAATCGCG+CGG + Chr5:99355595-99355614 MsG0580029609.01.T01:CDS 70.0%
! CGCGCTGGTGGCGCGTGGAA+CGG + Chr5:99355542-99355561 MsG0580029609.01.T01:CDS 75.0%
! CTCTACGCGCTGGTGGCGCG+TGG + Chr5:99355537-99355556 MsG0580029609.01.T01:CDS 75.0%
! GCTGGTGGCGCGTGGAACGG+TGG + Chr5:99355545-99355564 MsG0580029609.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr5 gene 99355350 99358872 99355350 ID=MsG0580029609.01;Name=MsG0580029609.01
Chr5 mRNA 99355350 99358872 99355350 ID=MsG0580029609.01.T01;Parent=MsG0580029609.01;Name=MsG0580029609.01.T01;_AED=0.49;_eAED=0.57;_QI=178|0|0.6|1|1|1|5|407|291
Chr5 exon 99355350 99355935 99355350 ID=MsG0580029609.01.T01:exon:36209;Parent=MsG0580029609.01.T01
Chr5 exon 99356196 99356324 99356196 ID=MsG0580029609.01.T01:exon:36210;Parent=MsG0580029609.01.T01
Chr5 exon 99356728 99356891 99356728 ID=MsG0580029609.01.T01:exon:36211;Parent=MsG0580029609.01.T01
Chr5 exon 99357562 99357648 99357562 ID=MsG0580029609.01.T01:exon:36212;Parent=MsG0580029609.01.T01
Chr5 exon 99358378 99358872 99358378 ID=MsG0580029609.01.T01:exon:36213;Parent=MsG0580029609.01.T01
Chr5 five_prime_UTR 99355350 99355527 99355350 ID=MsG0580029609.01.T01:five_prime_utr;Parent=MsG0580029609.01.T01
Chr5 CDS 99355528 99355935 99355528 ID=MsG0580029609.01.T01:cds;Parent=MsG0580029609.01.T01
Chr5 CDS 99356196 99356324 99356196 ID=MsG0580029609.01.T01:cds;Parent=MsG0580029609.01.T01
Chr5 CDS 99356728 99356891 99356728 ID=MsG0580029609.01.T01:cds;Parent=MsG0580029609.01.T01
Chr5 CDS 99357562 99357648 99357562 ID=MsG0580029609.01.T01:cds;Parent=MsG0580029609.01.T01
Chr5 CDS 99358378 99358465 99358378 ID=MsG0580029609.01.T01:cds;Parent=MsG0580029609.01.T01
Chr5 three_prime_UTR 99358466 99358872 99358466 ID=MsG0580029609.01.T01:three_prime_utr;Parent=MsG0580029609.01.T01
Gene Sequence

>MsG0580029609.01.T01

ATGGGTATACTCTACGCGCTGGTGGCGCGTGGAACGGTGGTTCTCGCGGAATTCACCGGAACAACAACAAACGCCGCCGCAATCGCGCGGCAAATCCTCGAGAAAATCCCCGGAAACAACGATACACATGTTTCGTATTCACAAGATCGGTATATTTTTCACGTGAAACGAACCGATGGACTCACGGTGCTGTGTATGGCGGATGATAGCGTTGGACGGAGAATTCCGTTTGCGTTTCTTGAAGAGATTCATCAGAGGTTTGTAAGGAGTTATGGACGTGCGGTTTTGACTGCGGAAGCATATGCTATGAATGATGAATTTTCTAGGGTTTTGAATCAGCAGATGGAGTATTTCTCCAGTGATCCGAATGCTGATAGGATTAATCGGCTTAAAGGTGAAATGTCTCAGATTATGATGGCTTATGGTGATTCTGCCAAATTCACTGTCAGTCCAAACATATGTGTGCGTGTTTGGATTCATTTTTGGGAGAGTCAAAAGTTATTCTGTAGTGGTGAAATTGATTTTGATATGTTTGGGGTAAGAAATGTTATGATAGAAAATATTGACAAAGTTTTGGATAGAGGTGATAGATTGGAATTGCTGGTGGATAAAGCTGCTAATATGCAAGGAAATACTTTTCGCTTCAAAAAGCAAGCTCGTCGCTTTAGAAGCACTGTCTGGTGGAGGAATGTCAAGCTCACTCACAATTGTGCCCGCAATGCGGTGAAAAATATGATATCCCATGACCAATTCAATGCGGTCAAAACTATCACCCTTGTAGATGCAGGGATTGCACTTATAGTCATCCTACTTGTGATAGCATATGTTGTGTTGGCTTTTGTTTGCCATGGGCCTGCACTACCGTCTTGCTTCTAG
Protein sequence

>MsG0580029609.01.T01

MGILYALVARGTVVLAEFTGTTTNAAAIARQILEKIPGNNDTHVSYSQDRYIFHVKRTDGLTVLCMADDSVGRRIPFAFLEEIHQRFVRSYGRAVLTAEAYAMNDEFSRVLNQQMEYFSSDPNADRINRLKGEMSQIMMAYGDSAKFTVSPNICVRVWIHFWESQKLFCSGEIDFDMFGVRNVMIENIDKVLDRGDRLELLVDKAANMQGNTFRFKKQARRFRSTVWWRNVKLTHNCARNAVKNMISHDQFNAVKTITLVDAGIALIVILLVIAYVVLAFVCHGPALPSCF*