AlfalfaGEDB | MsG0480022492.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022492.01

KEGG: K08486
GO: GO:0005484 GO:0006886 GO:0016020 GO:0016192
PFAM: PF00804 PF05739

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022492.01.T01	XP_003608218.1	94.574	258	4	1	1	248	48	305	1.71E-175	494
MsG0480022492.01.T02	XP_003608218.1	98.689	305	4	0	1	305	1	305	0	620
MsG0480022492.01.T03	XP_003608218.1	98.45	258	4	0	1	258	48	305	0	523
MsG0480022492.01.T04	XP_003608218.1	97.333	75	2	0	1	75	231	305	2.36E-41	147

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022492.01.T01	O64791	74.708	257	51	2	1	247	47	299	1.52E-138	394
MsG0480022492.01.T02	O64791	77.632	304	63	2	1	304	1	299	1.90E-177	495
MsG0480022492.01.T03	O64791	78.21	257	52	1	1	257	47	299	2.30E-149	422
MsG0480022492.01.T04	O64791	70.27	74	18	1	1	74	230	299	1.56E-27	103

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022492.01.T01	G7JRS4	94.574	258	4	1	1	248	48	305	8.15e-176	494
MsG0480022492.01.T02	G7JRS4	98.689	305	4	0	1	305	1	305	0.0	620
MsG0480022492.01.T03	G7JRS4	98.450	258	4	0	1	258	48	305	0.0	523
MsG0480022492.01.T04	G7JRS4	97.333	75	2	0	1	75	231	305	1.13e-41	147

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0380012445.01	MsG0480022492.01	PPI
MsG0380012445.01	MsG0480022492.01	PPI
MsG0380014303.01	MsG0480022492.01	PPI
MsG0380014303.01	MsG0480022492.01	PPI
MsG0480022492.01	MsG0580024472.01	PPI
MsG0480022492.01	MsG0580024472.01	PPI
MsG0580024489.01	MsG0480022492.01	PPI
MsG0580024489.01	MsG0480022492.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022492.01.T01	MTR_4g090900	94.574	258	4	1	1	248	48	305	2.07e-179	494
MsG0480022492.01.T01	MTR_2g012270	86.381	257	25	1	1	247	47	303	3.54e-164	456
MsG0480022492.01.T01	MTR_1g056550	59.740	231	83	1	1	221	55	285	2.99e-99	292
MsG0480022492.01.T01	MTR_1g056560	60.086	233	83	1	1	223	59	291	4.11e-99	293
MsG0480022492.01.T01	MTR_7g106420	57.940	233	88	1	1	223	42	274	2.32e-94	279
MsG0480022492.01.T01	MTR_5g012010	53.879	232	96	2	1	221	65	296	2.60e-82	249
MsG0480022492.01.T01	MTR_6g028140	50.000	230	105	1	4	223	51	280	2.14e-75	231
MsG0480022492.01.T01	MTR_2g088700	51.316	228	101	1	4	221	56	283	5.60e-75	230
MsG0480022492.01.T01	MTR_2g088700	46.586	249	123	1	4	242	56	304	5.20e-74	228
MsG0480022492.01.T01	MTR_5g020670	46.411	209	110	2	16	222	70	278	1.21e-59	191

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022492.01.T01	AT1G61290	74.708	257	51	2	1	247	47	299	1.55e-139	394
MsG0480022492.01.T01	AT1G61290	74.708	257	51	2	1	247	47	299	1.55e-139	394
MsG0480022492.01.T01	AT1G11250	78.112	233	41	1	1	223	42	274	7.09e-133	377
MsG0480022492.01.T01	AT4G03330	68.775	253	69	1	1	243	50	302	1.75e-118	340
MsG0480022492.01.T01	AT3G11820	64.502	231	72	1	1	221	27	257	1.02e-103	303
MsG0480022492.01.T01	AT3G11820	64.502	231	72	1	1	221	58	288	3.19e-103	303
MsG0480022492.01.T01	AT3G52400	57.940	233	88	1	1	223	57	289	1.42e-88	266
MsG0480022492.01.T01	AT3G03800	50.450	222	100	1	10	221	58	279	1.11e-72	224
MsG0480022492.01.T01	AT3G03800	50.450	222	100	1	10	221	58	279	1.11e-72	224
MsG0480022492.01.T01	AT5G08080	52.294	218	94	1	14	221	61	278	1.54e-68	214

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Find 41 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAATTGAACAATTGTAT+AGG	0.433871	4:-73200551	MsG0480022492.01.T03:CDS
AAACGTGTCAAAATTATTAA+AGG	0.208067	4:-73200429	MsG0480022492.01.T03:CDS
AAAGTGAAAATTTCATGCAA+AGG	0.560085	4:-73200149	MsG0480022492.01.T03:CDS
AACAAAAGAATTCTAGGAAG+TGG	0.524800	4:-73199891	MsG0480022492.01.T03:CDS
AACATTCCAGGGTGTGGTCC+AGG	0.464073	4:-73200366	MsG0480022492.01.T03:CDS
AAGAACTAGCATGTGCAACA+TGG	0.643271	4:+73199956	None:intergenic
AAGAGGGACGGAACAACTTC+AGG	0.385821	4:-73199926	MsG0480022492.01.T03:CDS
AAGTGAAAATTTCATGCAAA+GGG	0.501636	4:-73200148	MsG0480022492.01.T03:CDS
AATAGAAACATTCCAGGGTG+TGG	0.577755	4:-73200372	MsG0480022492.01.T03:CDS
ACAAGGTAGGGGTCAAATAA+TGG	0.355079	4:-73200115	MsG0480022492.01.T03:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATGTTATGCCATAATTCT+TGG	-	Chr4:73200629-73200648	MsG0480022492.01.T02:CDS	30.0%
GAAGAAACTCAAAGATATGA+TGG	-	Chr4:73200199-73200218	MsG0480022492.01.T03:CDS	30.0%
ACTAGAACTTCAGTTGTTAG+TGG	-	Chr4:73200170-73200189	MsG0480022492.01.T03:CDS	35.0%
ACTTCAGTTGTTAGTGGTTT+AGG	-	Chr4:73200176-73200195	MsG0480022492.01.T03:CDS	35.0%
AGAAAGACATGATGCTGTTA+AGG	-	Chr4:73200424-73200443	MsG0480022492.01.T03:CDS	35.0%
CAGCTAATAGAAACATTCCA+GGG	-	Chr4:73200120-73200139	MsG0480022492.01.T03:CDS	35.0%
CGATAAATTCTTCGAAGATG+TGG	-	Chr4:73199902-73199921	MsG0480022492.01.T03:CDS	35.0%
CTTCAGTTGTTAGTGGTTTA+GGG	-	Chr4:73200177-73200196	MsG0480022492.01.T03:CDS	35.0%
GAGTGCAACAAAAGAATTCT+AGG	-	Chr4:73200600-73200619	MsG0480022492.01.T02:CDS	35.0%
GCATCTTGAGTATAAGGAAA+CGG	-	Chr4:73200250-73200269	MsG0480022492.01.T03:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	73199801	73200718	73199801	ID=MsG0480022492.01;Name=MsG0480022492.01
Chr4	mRNA	73199801	73200577	73199801	ID=MsG0480022492.01.T01;Parent=MsG0480022492.01;Name=MsG0480022492.01.T01;_AED=0.10;_eAED=0.10;_QI=0\|0\|0\|1\|0\|0\|2\|0\|248
Chr4	exon	73199801	73200102	73199801	ID=MsG0480022492.01.T01:exon:10280;Parent=MsG0480022492.01.T01
Chr4	exon	73200133	73200577	73200133	ID=MsG0480022492.01.T01:exon:10279;Parent=MsG0480022492.01.T01
Chr4	CDS	73200133	73200577	73200133	ID=MsG0480022492.01.T01:cds;Parent=MsG0480022492.01.T01
Chr4	CDS	73199801	73200102	73199801	ID=MsG0480022492.01.T01:cds;Parent=MsG0480022492.01.T01
Chr4	mRNA	73199801	73200718	73199801	ID=MsG0480022492.01.T02;Parent=MsG0480022492.01;Name=MsG0480022492.01.T02;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|305
Chr4	exon	73199801	73200718	73199801	ID=MsG0480022492.01.T02:exon:10281;Parent=MsG0480022492.01.T02
Chr4	CDS	73199801	73200718	73199801	ID=MsG0480022492.01.T02:cds;Parent=MsG0480022492.01.T02
Chr4	mRNA	73199801	73200577	73199801	ID=MsG0480022492.01.T03;Parent=MsG0480022492.01;Name=MsG0480022492.01.T03;_AED=0.08;_eAED=0.08;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|258
Chr4	exon	73199801	73200577	73199801	ID=MsG0480022492.01.T03:exon:10282;Parent=MsG0480022492.01.T03
Chr4	CDS	73199801	73200577	73199801	ID=MsG0480022492.01.T03:cds;Parent=MsG0480022492.01.T03
Chr4	mRNA	73199801	73200028	73199801	ID=MsG0480022492.01.T04;Parent=MsG0480022492.01;Name=MsG0480022492.01.T04;_AED=0.38;_eAED=0.38;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|75
Chr4	exon	73199801	73200028	73199801	ID=MsG0480022492.01.T04:exon:10283;Parent=MsG0480022492.01.T04
Chr4	CDS	73199801	73200028	73199801	ID=MsG0480022492.01.T04:cds;Parent=MsG0480022492.01.T04

Gene Sequence

>MsG0480022492.01.T02

ATGAACGACTTGTTCTCAAATTCCTTCAAAAAGTACACTGATTTGAAGGAACAAGCTTACCTTGATGATGTCGAGGCAGGGAATAGAGAAACTGTCAATCTCGATAAATTCTTCGAAGATGTGGAGAATATAAAAGAAGACATGAAAACAATTGAACAATTGTATAGGAAATTGAAAGAGGCTAATGAAGAAAGTAAAACCGTGCACAATGCTAAAACCATGAAAGAACTTCGAGCACGAATGGACAAAGATGTTGAACAAGTCCTTAAACGTGTCAAAATTATTAAAGGAAAACTCGAAGCATTGGATCGTTCAAATGCAGCTAATAGAAACATTCCAGGGTGTGGTCCAGGTTCATCAGCAGATAGAACTAGAACTTCAGTTGTTAGTGGTTTAGGGAAGAAACTCAAAGATATGATGGATGATTTTCAAGGTTTGAGAGCAAAAATGCATCTTGAGTATAAGGAAACGGTGGAACGAAGATATTTTACAATAACTGGAGAGAAAGCGGATGAAGAAACTATAGAAAATTTGATATCAAGTGGAGAAAGTGAAAATTTCATGCAAAGGGCAATTCAAGAACAAGGTAGGGGTCAAATAATGGACACTATTTCGGAAATTCAAGAAAGACATGATGCTGTTAAGGAAATAGAGAAGAATTTGATCGAGTTGCATCAAGTGTTTTTGGATATGGCAGCTTTGGTAGAGTCTCAAGGACAACAACTTAATAATATTGAGAGCCATGTTGCACATGCTAGTTCTTTTGTAAGAAGAGGGACGGAACAACTTCAGGAAGCTAGAGTGCAACAAAAGAATTCTAGGAAGTGGACATGTTATGCCATAATTCTTGGTATTGTTCTAGTTGTTGTGCTTTTGTTTCCTCTCTTGACATCATTTTTACCTCATTTGTTAAAATGA

>MsG0480022492.01.T04

ATGGCAGCTTTGGTAGAGTCTCAAGGACAACAACTTAATAATATTGAGAGCCATGTTGCACATGCTAGTTCTTTTGTAAGAAGAGGGACGGAACAACTTCAGGAAGCTAGAGTGCAACAAAAGAATTCTAGGAAGTGGACATGTTATGCCATAATTCTTGGTATTGTTCTAGTTGTTGTGCTTTTGTTTCCTCTCTTGACATCATTTTTACCTCATTTGTTAAAATGA

>MsG0480022492.01.T01

ATGAAAACAATTGAACAATTGTATAGGAAATTGAAAGAGGCTAATGAAGAAAGTAAAACCGTGCACAATGCTAAAACCATGAAAGAACTTCGAGCACGAATGGACAAAGATGTTGAACAAGTCCTTAAACGTGTCAAAATTATTAAAGGAAAACTCGAAGCATTGGATCGTTCAAATGCAGCTAATAGAAACATTCCAGGGTGTGGTCCAGGTTCATCAGCAGATAGAACTAGAACTTCAGTTGTTAGTGGTTTAGGGAAGAAACTCAAAGATATGATGGATGATTTTCAAGGTTTGAGAGCAAAAATGCATCTTGAGTATAAGGAAACGGTGGAACGAAGATATTTTACAATAACTGGAGAGAAAGCGGATGAAGAAACTATAGAAAATTTGATATCAAGTGGAGAAAGTGAAAATTTCATGCAAAGGGCAATTCAAGAACAAGAAATTCAAGAAAGACATGATGCTGTTAAGGAAATAGAGAAGAATTTGATCGAGTTGCATCAAGTGTTTTTGGATATGGCAGCTTTGGTAGAGTCTCAAGGACAACAACTTAATAATATTGAGAGCCATGTTGCACATGCTAGTTCTTTTGTAAGAAGAGGGACGGAACAACTTCAGGAAGCTAGAGTGCAACAAAAGAATTCTAGGAAGTGGACATGTTATGCCATAATTCTTGGTATTGTTCTAGTTGTTGTGCTTTTGTTTCCTCTCTTGACATCATTTTTACCTCATTTGTTAAAATGA

>MsG0480022492.01.T03

ATGAAAACAATTGAACAATTGTATAGGAAATTGAAAGAGGCTAATGAAGAAAGTAAAACCGTGCACAATGCTAAAACCATGAAAGAACTTCGAGCACGAATGGACAAAGATGTTGAACAAGTCCTTAAACGTGTCAAAATTATTAAAGGAAAACTCGAAGCATTGGATCGTTCAAATGCAGCTAATAGAAACATTCCAGGGTGTGGTCCAGGTTCATCAGCAGATAGAACTAGAACTTCAGTTGTTAGTGGTTTAGGGAAGAAACTCAAAGATATGATGGATGATTTTCAAGGTTTGAGAGCAAAAATGCATCTTGAGTATAAGGAAACGGTGGAACGAAGATATTTTACAATAACTGGAGAGAAAGCGGATGAAGAAACTATAGAAAATTTGATATCAAGTGGAGAAAGTGAAAATTTCATGCAAAGGGCAATTCAAGAACAAGGTAGGGGTCAAATAATGGACACTATTTCGGAAATTCAAGAAAGACATGATGCTGTTAAGGAAATAGAGAAGAATTTGATCGAGTTGCATCAAGTGTTTTTGGATATGGCAGCTTTGGTAGAGTCTCAAGGACAACAACTTAATAATATTGAGAGCCATGTTGCACATGCTAGTTCTTTTGTAAGAAGAGGGACGGAACAACTTCAGGAAGCTAGAGTGCAACAAAAGAATTCTAGGAAGTGGACATGTTATGCCATAATTCTTGGTATTGTTCTAGTTGTTGTGCTTTTGTTTCCTCTCTTGACATCATTTTTACCTCATTTGTTAAAATGA

Protein sequence

>MsG0480022492.01.T02

MNDLFSNSFKKYTDLKEQAYLDDVEAGNRETVNLDKFFEDVENIKEDMKTIEQLYRKLKEANEESKTVHNAKTMKELRARMDKDVEQVLKRVKIIKGKLEALDRSNAANRNIPGCGPGSSADRTRTSVVSGLGKKLKDMMDDFQGLRAKMHLEYKETVERRYFTITGEKADEETIENLISSGESENFMQRAIQEQGRGQIMDTISEIQERHDAVKEIEKNLIELHQVFLDMAALVESQGQQLNNIESHVAHASSFVRRGTEQLQEARVQQKNSRKWTCYAIILGIVLVVVLLFPLLTSFLPHLLK*

>MsG0480022492.01.T04

MAALVESQGQQLNNIESHVAHASSFVRRGTEQLQEARVQQKNSRKWTCYAIILGIVLVVVLLFPLLTSFLPHLLK*

>MsG0480022492.01.T01

MKTIEQLYRKLKEANEESKTVHNAKTMKELRARMDKDVEQVLKRVKIIKGKLEALDRSNAANRNIPGCGPGSSADRTRTSVVSGLGKKLKDMMDDFQGLRAKMHLEYKETVERRYFTITGEKADEETIENLISSGESENFMQRAIQEQEIQERHDAVKEIEKNLIELHQVFLDMAALVESQGQQLNNIESHVAHASSFVRRGTEQLQEARVQQKNSRKWTCYAIILGIVLVVVLLFPLLTSFLPHLLK*

>MsG0480022492.01.T03

MKTIEQLYRKLKEANEESKTVHNAKTMKELRARMDKDVEQVLKRVKIIKGKLEALDRSNAANRNIPGCGPGSSADRTRTSVVSGLGKKLKDMMDDFQGLRAKMHLEYKETVERRYFTITGEKADEETIENLISSGESENFMQRAIQEQGRGQIMDTISEIQERHDAVKEIEKNLIELHQVFLDMAALVESQGQQLNNIESHVAHASSFVRRGTEQLQEARVQQKNSRKWTCYAIILGIVLVVVLLFPLLTSFLPHLLK*