Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022492.01.T02 | XP_003608218.1 | 98.689 | 305 | 4 | 0 | 1 | 305 | 1 | 305 | 0 | 620 |
| MsG0480022492.01.T04 | XP_003608218.1 | 97.333 | 75 | 2 | 0 | 1 | 75 | 231 | 305 | 2.36E-41 | 147 |
| MsG0480022492.01.T01 | XP_003608218.1 | 94.574 | 258 | 4 | 1 | 1 | 248 | 48 | 305 | 1.71E-175 | 494 |
| MsG0480022492.01.T03 | XP_003608218.1 | 98.45 | 258 | 4 | 0 | 1 | 258 | 48 | 305 | 0 | 523 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022492.01.T01 | O64791 | 74.708 | 257 | 51 | 2 | 1 | 247 | 47 | 299 | 1.52E-138 | 394 |
| MsG0480022492.01.T02 | O64791 | 77.632 | 304 | 63 | 2 | 1 | 304 | 1 | 299 | 1.90E-177 | 495 |
| MsG0480022492.01.T03 | O64791 | 78.21 | 257 | 52 | 1 | 1 | 257 | 47 | 299 | 2.30E-149 | 422 |
| MsG0480022492.01.T04 | O64791 | 70.27 | 74 | 18 | 1 | 1 | 74 | 230 | 299 | 1.56E-27 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022492.01.T01 | G7JRS4 | 94.574 | 258 | 4 | 1 | 1 | 248 | 48 | 305 | 8.15e-176 | 494 |
| MsG0480022492.01.T02 | G7JRS4 | 98.689 | 305 | 4 | 0 | 1 | 305 | 1 | 305 | 0.0 | 620 |
| MsG0480022492.01.T03 | G7JRS4 | 98.450 | 258 | 4 | 0 | 1 | 258 | 48 | 305 | 0.0 | 523 |
| MsG0480022492.01.T04 | G7JRS4 | 97.333 | 75 | 2 | 0 | 1 | 75 | 231 | 305 | 1.13e-41 | 147 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480022492.01 | MsG0580024472.01 | PPI |
| MsG0580024489.01 | MsG0480022492.01 | PPI |
| MsG0380014303.01 | MsG0480022492.01 | PPI |
| MsG0480022492.01 | MsG0580024472.01 | PPI |
| MsG0580024489.01 | MsG0480022492.01 | PPI |
| MsG0380014303.01 | MsG0480022492.01 | PPI |
| MsG0380012445.01 | MsG0480022492.01 | PPI |
| MsG0380012445.01 | MsG0480022492.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022492.01.T02 | MTR_4g090900 | 98.689 | 305 | 4 | 0 | 1 | 305 | 1 | 305 | 0.0 | 620 |
| MsG0480022492.01.T02 | MTR_2g012270 | 88.816 | 304 | 33 | 1 | 1 | 304 | 1 | 303 | 0.0 | 563 |
| MsG0480022492.01.T02 | MTR_1g056550 | 58.741 | 286 | 109 | 3 | 1 | 278 | 1 | 285 | 1.04e-120 | 348 |
| MsG0480022492.01.T02 | MTR_1g056560 | 57.045 | 291 | 114 | 2 | 1 | 280 | 1 | 291 | 1.27e-118 | 345 |
| MsG0480022492.01.T02 | MTR_7g106420 | 55.000 | 280 | 120 | 1 | 1 | 280 | 1 | 274 | 1.13e-107 | 315 |
| MsG0480022492.01.T02 | MTR_5g012010 | 50.338 | 296 | 129 | 4 | 1 | 278 | 1 | 296 | 2.66e-99 | 295 |
| MsG0480022492.01.T02 | MTR_6g028140 | 48.239 | 284 | 139 | 2 | 1 | 280 | 1 | 280 | 7.75e-91 | 273 |
| MsG0480022492.01.T02 | MTR_2g088700 | 46.643 | 283 | 146 | 1 | 1 | 278 | 1 | 283 | 5.01e-87 | 263 |
| MsG0480022492.01.T02 | MTR_2g088700 | 43.092 | 304 | 168 | 1 | 1 | 299 | 1 | 304 | 3.05e-86 | 261 |
| MsG0480022492.01.T02 | MTR_5g020670 | 44.599 | 287 | 142 | 4 | 1 | 279 | 1 | 278 | 4.53e-72 | 224 |
| MsG0480022492.01.T02 | MTR_8g073900 | 40.476 | 168 | 71 | 3 | 1 | 161 | 1 | 146 | 1.56e-25 | 99.8 |
| MsG0480022492.01.T04 | MTR_4g090900 | 97.333 | 75 | 2 | 0 | 1 | 75 | 231 | 305 | 2.86e-45 | 147 |
| MsG0480022492.01.T04 | MTR_2g012270 | 85.135 | 74 | 11 | 0 | 1 | 74 | 230 | 303 | 1.68e-38 | 129 |
| MsG0480022492.01.T04 | MTR_1g056560 | 65.306 | 49 | 17 | 0 | 1 | 49 | 242 | 290 | 3.30e-17 | 73.9 |
| MsG0480022492.01.T04 | MTR_1g056550 | 64.583 | 48 | 17 | 0 | 1 | 48 | 238 | 285 | 1.89e-15 | 68.9 |
| MsG0480022492.01.T04 | MTR_7g106420 | 58.000 | 50 | 21 | 0 | 1 | 50 | 225 | 274 | 1.29e-14 | 66.6 |
| MsG0480022492.01.T04 | MTR_5g012010 | 52.083 | 48 | 23 | 0 | 1 | 48 | 249 | 296 | 3.06e-12 | 60.1 |
| MsG0480022492.01.T04 | MTR_2g088700 | 60.417 | 48 | 19 | 0 | 1 | 48 | 236 | 283 | 3.41e-12 | 59.7 |
| MsG0480022492.01.T04 | MTR_6g028140 | 50.000 | 50 | 25 | 0 | 1 | 50 | 231 | 280 | 8.70e-11 | 55.8 |
| MsG0480022492.01.T01 | MTR_4g090900 | 94.574 | 258 | 4 | 1 | 1 | 248 | 48 | 305 | 2.07e-179 | 494 |
| MsG0480022492.01.T01 | MTR_2g012270 | 86.381 | 257 | 25 | 1 | 1 | 247 | 47 | 303 | 3.54e-164 | 456 |
| MsG0480022492.01.T01 | MTR_1g056550 | 59.740 | 231 | 83 | 1 | 1 | 221 | 55 | 285 | 2.99e-99 | 292 |
| MsG0480022492.01.T01 | MTR_1g056560 | 60.086 | 233 | 83 | 1 | 1 | 223 | 59 | 291 | 4.11e-99 | 293 |
| MsG0480022492.01.T01 | MTR_7g106420 | 57.940 | 233 | 88 | 1 | 1 | 223 | 42 | 274 | 2.32e-94 | 279 |
| MsG0480022492.01.T01 | MTR_5g012010 | 53.879 | 232 | 96 | 2 | 1 | 221 | 65 | 296 | 2.60e-82 | 249 |
| MsG0480022492.01.T01 | MTR_6g028140 | 50.000 | 230 | 105 | 1 | 4 | 223 | 51 | 280 | 2.14e-75 | 231 |
| MsG0480022492.01.T01 | MTR_2g088700 | 51.316 | 228 | 101 | 1 | 4 | 221 | 56 | 283 | 5.60e-75 | 230 |
| MsG0480022492.01.T01 | MTR_2g088700 | 46.586 | 249 | 123 | 1 | 4 | 242 | 56 | 304 | 5.20e-74 | 228 |
| MsG0480022492.01.T01 | MTR_5g020670 | 46.411 | 209 | 110 | 2 | 16 | 222 | 70 | 278 | 1.21e-59 | 191 |
| MsG0480022492.01.T01 | MTR_8g073900 | 41.414 | 99 | 36 | 1 | 16 | 114 | 70 | 146 | 1.50e-15 | 72.0 |
| MsG0480022492.01.T03 | MTR_4g090900 | 98.450 | 258 | 4 | 0 | 1 | 258 | 48 | 305 | 0.0 | 523 |
| MsG0480022492.01.T03 | MTR_2g012270 | 90.272 | 257 | 25 | 0 | 1 | 257 | 47 | 303 | 1.82e-175 | 485 |
| MsG0480022492.01.T03 | MTR_1g056560 | 63.090 | 233 | 86 | 0 | 1 | 233 | 59 | 291 | 1.44e-108 | 317 |
| MsG0480022492.01.T03 | MTR_1g056550 | 62.771 | 231 | 86 | 0 | 1 | 231 | 55 | 285 | 4.06e-108 | 315 |
| MsG0480022492.01.T03 | MTR_7g106420 | 59.657 | 233 | 94 | 0 | 1 | 233 | 42 | 274 | 7.18e-101 | 296 |
| MsG0480022492.01.T03 | MTR_5g012010 | 55.603 | 232 | 102 | 1 | 1 | 231 | 65 | 296 | 4.23e-90 | 270 |
| MsG0480022492.01.T03 | MTR_6g028140 | 53.043 | 230 | 108 | 0 | 4 | 233 | 51 | 280 | 9.82e-86 | 258 |
| MsG0480022492.01.T03 | MTR_2g088700 | 53.509 | 228 | 106 | 0 | 4 | 231 | 56 | 283 | 1.17e-82 | 250 |
| MsG0480022492.01.T03 | MTR_2g088700 | 48.594 | 249 | 128 | 0 | 4 | 252 | 56 | 304 | 6.05e-82 | 248 |
| MsG0480022492.01.T03 | MTR_5g020670 | 44.954 | 218 | 110 | 2 | 16 | 232 | 70 | 278 | 6.01e-59 | 189 |
| MsG0480022492.01.T03 | MTR_8g073900 | 41.414 | 99 | 36 | 1 | 16 | 114 | 70 | 146 | 1.69e-15 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022492.01.T02 | AT1G61290 | 77.632 | 304 | 63 | 2 | 1 | 304 | 1 | 299 | 1.94e-178 | 495 |
| MsG0480022492.01.T02 | AT1G61290 | 77.632 | 304 | 63 | 2 | 1 | 304 | 1 | 299 | 1.94e-178 | 495 |
| MsG0480022492.01.T02 | AT1G11250 | 80.357 | 280 | 49 | 2 | 1 | 280 | 1 | 274 | 6.59e-166 | 463 |
| MsG0480022492.01.T02 | AT4G03330 | 69.536 | 302 | 90 | 1 | 1 | 300 | 1 | 302 | 6.90e-149 | 420 |
| MsG0480022492.01.T02 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 5 | 257 | 3.18e-124 | 358 |
| MsG0480022492.01.T02 | AT3G11820 | 67.194 | 253 | 83 | 0 | 26 | 278 | 36 | 288 | 1.41e-123 | 357 |
| MsG0480022492.01.T02 | AT3G52400 | 55.973 | 293 | 112 | 3 | 1 | 280 | 1 | 289 | 5.71e-107 | 315 |
| MsG0480022492.01.T02 | AT3G03800 | 47.872 | 282 | 140 | 2 | 1 | 278 | 1 | 279 | 4.22e-88 | 266 |
| MsG0480022492.01.T02 | AT3G03800 | 47.872 | 282 | 140 | 2 | 1 | 278 | 1 | 279 | 4.22e-88 | 266 |
| MsG0480022492.01.T02 | AT1G08560 | 46.735 | 291 | 137 | 4 | 1 | 278 | 1 | 286 | 4.51e-86 | 261 |
| MsG0480022492.01.T02 | AT5G08080 | 49.643 | 280 | 137 | 2 | 1 | 278 | 1 | 278 | 2.22e-81 | 248 |
| MsG0480022492.01.T02 | AT5G08080 | 49.643 | 280 | 137 | 2 | 1 | 278 | 1 | 278 | 2.22e-81 | 248 |
| MsG0480022492.01.T02 | AT5G08080 | 47.423 | 291 | 138 | 4 | 1 | 278 | 1 | 289 | 2.94e-79 | 243 |
| MsG0480022492.01.T02 | AT2G18260 | 36.977 | 311 | 176 | 5 | 1 | 298 | 1 | 304 | 8.99e-64 | 203 |
| MsG0480022492.01.T02 | AT2G18260 | 36.977 | 311 | 176 | 5 | 1 | 298 | 1 | 304 | 8.99e-64 | 203 |
| MsG0480022492.01.T02 | AT5G08080 | 46.040 | 202 | 105 | 2 | 1 | 200 | 1 | 200 | 2.93e-44 | 150 |
| MsG0480022492.01.T02 | AT5G08080 | 46.040 | 202 | 105 | 2 | 1 | 200 | 1 | 200 | 2.93e-44 | 150 |
| MsG0480022492.01.T04 | AT1G61290 | 70.270 | 74 | 18 | 1 | 1 | 74 | 230 | 299 | 1.59e-28 | 103 |
| MsG0480022492.01.T04 | AT1G61290 | 70.270 | 74 | 18 | 1 | 1 | 74 | 230 | 299 | 1.59e-28 | 103 |
| MsG0480022492.01.T04 | AT1G11250 | 86.000 | 50 | 7 | 0 | 1 | 50 | 225 | 274 | 3.27e-24 | 92.4 |
| MsG0480022492.01.T04 | AT3G11820 | 72.917 | 48 | 13 | 0 | 1 | 48 | 241 | 288 | 4.98e-19 | 79.0 |
| MsG0480022492.01.T04 | AT3G11820 | 72.917 | 48 | 13 | 0 | 1 | 48 | 210 | 257 | 8.55e-19 | 78.2 |
| MsG0480022492.01.T04 | AT4G03330 | 62.857 | 70 | 26 | 0 | 1 | 70 | 233 | 302 | 6.83e-17 | 72.8 |
| MsG0480022492.01.T04 | AT3G52400 | 58.000 | 50 | 21 | 0 | 1 | 50 | 240 | 289 | 3.83e-14 | 65.5 |
| MsG0480022492.01.T04 | AT1G08560 | 52.083 | 48 | 23 | 0 | 1 | 48 | 239 | 286 | 3.79e-12 | 59.7 |
| MsG0480022492.01.T04 | AT2G18260 | 39.706 | 68 | 41 | 0 | 1 | 68 | 237 | 304 | 4.37e-12 | 59.7 |
| MsG0480022492.01.T04 | AT2G18260 | 39.706 | 68 | 41 | 0 | 1 | 68 | 237 | 304 | 4.37e-12 | 59.7 |
| MsG0480022492.01.T04 | AT5G08080 | 54.167 | 48 | 22 | 0 | 1 | 48 | 231 | 278 | 4.91e-11 | 56.6 |
| MsG0480022492.01.T04 | AT5G08080 | 54.167 | 48 | 22 | 0 | 1 | 48 | 231 | 278 | 4.91e-11 | 56.6 |
| MsG0480022492.01.T04 | AT5G08080 | 54.167 | 48 | 22 | 0 | 1 | 48 | 242 | 289 | 4.95e-11 | 56.6 |
| MsG0480022492.01.T01 | AT1G61290 | 74.708 | 257 | 51 | 2 | 1 | 247 | 47 | 299 | 1.55e-139 | 394 |
| MsG0480022492.01.T01 | AT1G61290 | 74.708 | 257 | 51 | 2 | 1 | 247 | 47 | 299 | 1.55e-139 | 394 |
| MsG0480022492.01.T01 | AT1G11250 | 78.112 | 233 | 41 | 1 | 1 | 223 | 42 | 274 | 7.09e-133 | 377 |
| MsG0480022492.01.T01 | AT4G03330 | 68.775 | 253 | 69 | 1 | 1 | 243 | 50 | 302 | 1.75e-118 | 340 |
| MsG0480022492.01.T01 | AT3G11820 | 64.502 | 231 | 72 | 1 | 1 | 221 | 27 | 257 | 1.02e-103 | 303 |
| MsG0480022492.01.T01 | AT3G11820 | 64.502 | 231 | 72 | 1 | 1 | 221 | 58 | 288 | 3.19e-103 | 303 |
| MsG0480022492.01.T01 | AT3G52400 | 57.940 | 233 | 88 | 1 | 1 | 223 | 57 | 289 | 1.42e-88 | 266 |
| MsG0480022492.01.T01 | AT3G03800 | 50.450 | 222 | 100 | 1 | 10 | 221 | 58 | 279 | 1.11e-72 | 224 |
| MsG0480022492.01.T01 | AT3G03800 | 50.450 | 222 | 100 | 1 | 10 | 221 | 58 | 279 | 1.11e-72 | 224 |
| MsG0480022492.01.T01 | AT5G08080 | 52.294 | 218 | 94 | 1 | 14 | 221 | 61 | 278 | 1.54e-68 | 214 |
| MsG0480022492.01.T01 | AT5G08080 | 52.294 | 218 | 94 | 1 | 14 | 221 | 61 | 278 | 1.54e-68 | 214 |
| MsG0480022492.01.T01 | AT5G08080 | 52.294 | 218 | 94 | 1 | 14 | 221 | 72 | 289 | 1.95e-68 | 214 |
| MsG0480022492.01.T01 | AT1G08560 | 48.276 | 232 | 105 | 3 | 1 | 221 | 59 | 286 | 1.13e-67 | 211 |
| MsG0480022492.01.T01 | AT2G18260 | 37.860 | 243 | 144 | 2 | 4 | 241 | 64 | 304 | 5.64e-55 | 179 |
| MsG0480022492.01.T01 | AT2G18260 | 37.860 | 243 | 144 | 2 | 4 | 241 | 64 | 304 | 5.64e-55 | 179 |
| MsG0480022492.01.T01 | AT5G08080 | 52.593 | 135 | 64 | 0 | 14 | 148 | 61 | 195 | 2.46e-37 | 131 |
| MsG0480022492.01.T01 | AT5G08080 | 52.593 | 135 | 64 | 0 | 14 | 148 | 61 | 195 | 2.46e-37 | 131 |
| MsG0480022492.01.T03 | AT1G61290 | 78.210 | 257 | 52 | 1 | 1 | 257 | 47 | 299 | 2.35e-150 | 422 |
| MsG0480022492.01.T03 | AT1G61290 | 78.210 | 257 | 52 | 1 | 1 | 257 | 47 | 299 | 2.35e-150 | 422 |
| MsG0480022492.01.T03 | AT1G11250 | 81.974 | 233 | 42 | 0 | 1 | 233 | 42 | 274 | 1.31e-143 | 404 |
| MsG0480022492.01.T03 | AT4G03330 | 71.937 | 253 | 71 | 0 | 1 | 253 | 50 | 302 | 2.41e-129 | 369 |
| MsG0480022492.01.T03 | AT3G11820 | 67.100 | 231 | 76 | 0 | 1 | 231 | 27 | 257 | 8.42e-113 | 327 |
| MsG0480022492.01.T03 | AT3G11820 | 67.100 | 231 | 76 | 0 | 1 | 231 | 58 | 288 | 2.61e-112 | 327 |
| MsG0480022492.01.T03 | AT3G52400 | 60.944 | 233 | 91 | 0 | 1 | 233 | 57 | 289 | 5.30e-98 | 290 |
| MsG0480022492.01.T03 | AT3G03800 | 54.054 | 222 | 102 | 0 | 10 | 231 | 58 | 279 | 7.68e-83 | 250 |
| MsG0480022492.01.T03 | AT3G03800 | 54.054 | 222 | 102 | 0 | 10 | 231 | 58 | 279 | 7.68e-83 | 250 |
| MsG0480022492.01.T03 | AT5G08080 | 55.505 | 218 | 97 | 0 | 14 | 231 | 72 | 289 | 1.78e-78 | 239 |
| MsG0480022492.01.T03 | AT5G08080 | 55.505 | 218 | 97 | 0 | 14 | 231 | 61 | 278 | 1.82e-78 | 239 |
| MsG0480022492.01.T03 | AT5G08080 | 55.505 | 218 | 97 | 0 | 14 | 231 | 61 | 278 | 1.82e-78 | 239 |
| MsG0480022492.01.T03 | AT1G08560 | 49.569 | 232 | 112 | 2 | 1 | 231 | 59 | 286 | 5.88e-75 | 231 |
| MsG0480022492.01.T03 | AT2G18260 | 37.500 | 248 | 148 | 3 | 4 | 251 | 64 | 304 | 7.54e-54 | 176 |
| MsG0480022492.01.T03 | AT2G18260 | 37.500 | 248 | 148 | 3 | 4 | 251 | 64 | 304 | 7.54e-54 | 176 |
| MsG0480022492.01.T03 | AT5G08080 | 53.571 | 140 | 65 | 0 | 14 | 153 | 61 | 200 | 4.08e-41 | 141 |
| MsG0480022492.01.T03 | AT5G08080 | 53.571 | 140 | 65 | 0 | 14 | 153 | 61 | 200 | 4.08e-41 | 141 |
Find 41 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCAGTTGTTAGTGGTTT+AGG | 0.190941 | 4:-73200321 | MsG0480022492.01.T03:CDS |
| AAACGTGTCAAAATTATTAA+AGG | 0.208067 | 4:-73200429 | MsG0480022492.01.T03:CDS |
| CTTCAGTTGTTAGTGGTTTA+GGG | 0.301650 | 4:-73200320 | MsG0480022492.01.T03:CDS |
| TCAAATAATGGACACTATTT+CGG | 0.305761 | 4:-73200103 | MsG0480022492.01.T03:CDS |
| GAGTGCAACAAAAGAATTCT+AGG | 0.314563 | 4:-73199897 | MsG0480022492.01.T03:CDS |
| TCGTGCTCGAAGTTCTTTCA+TGG | 0.315609 | 4:+73200479 | None:intergenic |
| ACATGTTATGCCATAATTCT+TGG | 0.328356 | 4:-73199868 | MsG0480022492.01.T03:CDS |
| TAGAACAATACCAAGAATTA+TGG | 0.331526 | 4:+73199858 | None:intergenic |
| ACAAGGTAGGGGTCAAATAA+TGG | 0.355079 | 4:-73200115 | MsG0480022492.01.T03:CDS |
| AAGAGGGACGGAACAACTTC+AGG | 0.385821 | 4:-73199926 | MsG0480022492.01.T03:CDS |
| AGAAAGACATGATGCTGTTA+AGG | 0.392149 | 4:-73200073 | MsG0480022492.01.T03:CDS |
| ACTAGAACTTCAGTTGTTAG+TGG | 0.396328 | 4:-73200327 | MsG0480022492.01.T03:CDS |
| AAACAATTGAACAATTGTAT+AGG | 0.433871 | 4:-73200551 | MsG0480022492.01.T03:CDS |
| TACCTTGATGATGTCGAGGC+AGG | 0.443621 | 4:-73200639 | MsG0480022492.01.T02:CDS |
| GCAGCTAATAGAAACATTCC+AGG | 0.458362 | 4:-73200378 | MsG0480022492.01.T03:CDS |
| TAAAGGAAAACTCGAAGCAT+TGG | 0.462694 | 4:-73200412 | MsG0480022492.01.T03:CDS |
| AACATTCCAGGGTGTGGTCC+AGG | 0.464073 | 4:-73200366 | MsG0480022492.01.T03:CDS |
| GAAGAAACTCAAAGATATGA+TGG | 0.479536 | 4:-73200298 | MsG0480022492.01.T03:CDS |
| GTTCTATCTGCTGATGAACC+TGG | 0.498169 | 4:+73200348 | None:intergenic |
| AAGTGAAAATTTCATGCAAA+GGG | 0.501636 | 4:-73200148 | MsG0480022492.01.T03:CDS |
| GGCAATTCAAGAACAAGGTA+GGG | 0.504105 | 4:-73200127 | MsG0480022492.01.T03:CDS |
| AACAAAAGAATTCTAGGAAG+TGG | 0.524800 | 4:-73199891 | MsG0480022492.01.T03:CDS |
| GGGCAATTCAAGAACAAGGT+AGG | 0.529449 | 4:-73200128 | MsG0480022492.01.T03:CDS |
| ATAGAAAATTTGATATCAAG+TGG | 0.532572 | 4:-73200174 | MsG0480022492.01.T03:CDS |
| ACCTTGATGATGTCGAGGCA+GGG | 0.538277 | 4:-73200638 | MsG0480022492.01.T02:CDS |
| TACAATAACTGGAGAGAAAG+CGG | 0.540895 | 4:-73200208 | MsG0480022492.01.T03:CDS |
| GAAAGAACTTCGAGCACGAA+TGG | 0.541312 | 4:-73200475 | MsG0480022492.01.T03:CDS |
| GCATCTTGAGTATAAGGAAA+CGG | 0.542829 | 4:-73200247 | MsG0480022492.01.T03:CDS |
| CGATAAATTCTTCGAAGATG+TGG | 0.548904 | 4:-73200595 | MsG0480022492.01.T02:CDS |
| ATTGTATAGGAAATTGAAAG+AGG | 0.552639 | 4:-73200538 | MsG0480022492.01.T03:CDS |
| GATGAACCTGGACCACACCC+TGG | 0.554068 | 4:+73200360 | None:intergenic |
| AAAGTGAAAATTTCATGCAA+AGG | 0.560085 | 4:-73200149 | MsG0480022492.01.T03:CDS |
| AATAGAAACATTCCAGGGTG+TGG | 0.577755 | 4:-73200372 | MsG0480022492.01.T03:CDS |
| TCCCTGCCTCGACATCATCA+AGG | 0.592333 | 4:+73200637 | None:intergenic |
| CAAAGGGCAATTCAAGAACA+AGG | 0.597990 | 4:-73200132 | MsG0480022492.01.T03:CDS |
| AGCTTACCTTGATGATGTCG+AGG | 0.625446 | 4:-73200643 | MsG0480022492.01.T02:CDS |
| GCAATTCAAGAACAAGGTAG+GGG | 0.626558 | 4:-73200126 | MsG0480022492.01.T03:CDS |
| GCAGCTTTGGTAGAGTCTCA+AGG | 0.630011 | 4:-73200003 | MsG0480022492.01.T03:CDS |
| AAGAACTAGCATGTGCAACA+TGG | 0.643271 | 4:+73199956 | None:intergenic |
| CAGCTAATAGAAACATTCCA+GGG | 0.687559 | 4:-73200377 | MsG0480022492.01.T03:CDS |
| TCTTGAGTATAAGGAAACGG+TGG | 0.715657 | 4:-73200244 | MsG0480022492.01.T03:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAATTGAACAATTGTAT+AGG | - | Chr4:73199946-73199965 | MsG0480022492.01.T03:CDS | 20.0% |
| !! | AAACGTGTCAAAATTATTAA+AGG | - | Chr4:73200068-73200087 | MsG0480022492.01.T03:CDS | 20.0% |
| !! | ATAGAAAATTTGATATCAAG+TGG | - | Chr4:73200323-73200342 | MsG0480022492.01.T03:CDS | 20.0% |
| !!! | TAATAATTTTGACACGTTTA+AGG | + | Chr4:73200067-73200086 | None:intergenic | 20.0% |
| ! | AAAAATGCATCTTGAGTATA+AGG | - | Chr4:73200244-73200263 | MsG0480022492.01.T03:CDS | 25.0% |
| ! | AAAGTGAAAATTTCATGCAA+AGG | - | Chr4:73200348-73200367 | MsG0480022492.01.T03:CDS | 25.0% |
| ! | AAGTGAAAATTTCATGCAAA+GGG | - | Chr4:73200349-73200368 | MsG0480022492.01.T03:CDS | 25.0% |
| ! | ATTGTATAGGAAATTGAAAG+AGG | - | Chr4:73199959-73199978 | MsG0480022492.01.T03:CDS | 25.0% |
| ! | TAGAACAATACCAAGAATTA+TGG | + | Chr4:73200642-73200661 | None:intergenic | 25.0% |
| !! | CGAAGATATTTTACAATAAC+TGG | - | Chr4:73200278-73200297 | MsG0480022492.01.T03:CDS | 25.0% |
| !!! | TCAAATAATGGACACTATTT+CGG | - | Chr4:73200394-73200413 | MsG0480022492.01.T03:CDS | 25.0% |
| ACATGTTATGCCATAATTCT+TGG | - | Chr4:73200629-73200648 | MsG0480022492.01.T02:CDS | 30.0% | |
| GAAGAAACTCAAAGATATGA+TGG | - | Chr4:73200199-73200218 | MsG0480022492.01.T03:CDS | 30.0% | |
| ! | AACAAAAGAATTCTAGGAAG+TGG | - | Chr4:73200606-73200625 | MsG0480022492.01.T02:CDS | 30.0% |
| !! | CAAAAAGTACACTGATTTGA+AGG | - | Chr4:73199827-73199846 | MsG0480022492.01.T03:CDS | 30.0% |
| !! | CAAATCAGTGTACTTTTTGA+AGG | + | Chr4:73199826-73199845 | None:intergenic | 30.0% |
| !! | CTAGTTCTTTTGTAAGAAGA+GGG | - | Chr4:73200555-73200574 | MsG0480022492.01.T03:CDS | 30.0% |
| !!! | GATATGATGGATGATTTTCA+AGG | - | Chr4:73200212-73200231 | MsG0480022492.01.T03:CDS | 30.0% |
| ACTAGAACTTCAGTTGTTAG+TGG | - | Chr4:73200170-73200189 | MsG0480022492.01.T03:CDS | 35.0% | |
| ACTTCAGTTGTTAGTGGTTT+AGG | - | Chr4:73200176-73200195 | MsG0480022492.01.T03:CDS | 35.0% | |
| AGAAAGACATGATGCTGTTA+AGG | - | Chr4:73200424-73200443 | MsG0480022492.01.T03:CDS | 35.0% | |
| CAGCTAATAGAAACATTCCA+GGG | - | Chr4:73200120-73200139 | MsG0480022492.01.T03:CDS | 35.0% | |
| CGATAAATTCTTCGAAGATG+TGG | - | Chr4:73199902-73199921 | MsG0480022492.01.T03:CDS | 35.0% | |
| CTTCAGTTGTTAGTGGTTTA+GGG | - | Chr4:73200177-73200196 | MsG0480022492.01.T03:CDS | 35.0% | |
| GAGTGCAACAAAAGAATTCT+AGG | - | Chr4:73200600-73200619 | MsG0480022492.01.T02:CDS | 35.0% | |
| GCATCTTGAGTATAAGGAAA+CGG | - | Chr4:73200250-73200269 | MsG0480022492.01.T03:CDS | 35.0% | |
| GGTAAAAATGATGTCAAGAG+AGG | + | Chr4:73200683-73200702 | None:intergenic | 35.0% | |
| TAAAGGAAAACTCGAAGCAT+TGG | - | Chr4:73200085-73200104 | MsG0480022492.01.T03:CDS | 35.0% | |
| TACAATAACTGGAGAGAAAG+CGG | - | Chr4:73200289-73200308 | MsG0480022492.01.T03:CDS | 35.0% | |
| ! | TTCTTTTGTAAGAAGAGGGA+CGG | - | Chr4:73200559-73200578 | MsG0480022492.01.T02:CDS | 35.0% |
| !! | GCTAGTTCTTTTGTAAGAAG+AGG | - | Chr4:73200554-73200573 | MsG0480022492.01.T03:CDS | 35.0% |
| !!! | GCATCAAGTGTTTTTGGATA+TGG | - | Chr4:73200472-73200491 | MsG0480022492.01.T03:CDS | 35.0% |
| AAGAACTAGCATGTGCAACA+TGG | + | Chr4:73200544-73200563 | None:intergenic | 40.0% | |
| AATAGAAACATTCCAGGGTG+TGG | - | Chr4:73200125-73200144 | MsG0480022492.01.T03:CDS | 40.0% | |
| ACAAGGTAGGGGTCAAATAA+TGG | - | Chr4:73200382-73200401 | MsG0480022492.01.T03:CDS | 40.0% | |
| CAAAGGGCAATTCAAGAACA+AGG | - | Chr4:73200365-73200384 | MsG0480022492.01.T03:CDS | 40.0% | |
| GCAATTCAAGAACAAGGTAG+GGG | - | Chr4:73200371-73200390 | MsG0480022492.01.T03:CDS | 40.0% | |
| GCAGCTAATAGAAACATTCC+AGG | - | Chr4:73200119-73200138 | MsG0480022492.01.T03:CDS | 40.0% | |
| GGCAATTCAAGAACAAGGTA+GGG | - | Chr4:73200370-73200389 | MsG0480022492.01.T03:CDS | 40.0% | |
| TCTTGAGTATAAGGAAACGG+TGG | - | Chr4:73200253-73200272 | MsG0480022492.01.T03:CDS | 40.0% | |
| ! | CGAGTTGCATCAAGTGTTTT+TGG | - | Chr4:73200466-73200485 | MsG0480022492.01.T03:CDS | 40.0% |
| !! | GTTTTTGGATATGGCAGCTT+TGG | - | Chr4:73200481-73200500 | MsG0480022492.01.T03:CDS | 40.0% |
| !!! | CATGGTTTTAGCATTGTGCA+CGG | + | Chr4:73200003-73200022 | None:intergenic | 40.0% |
| GAAAGAACTTCGAGCACGAA+TGG | - | Chr4:73200022-73200041 | MsG0480022492.01.T03:CDS | 45.0% | |
| GGGCAATTCAAGAACAAGGT+AGG | - | Chr4:73200369-73200388 | MsG0480022492.01.T03:CDS | 45.0% | |
| GTTCTATCTGCTGATGAACC+TGG | + | Chr4:73200152-73200171 | None:intergenic | 45.0% | |
| TCGTGCTCGAAGTTCTTTCA+TGG | + | Chr4:73200021-73200040 | None:intergenic | 45.0% | |
| !! | AGCTTACCTTGATGATGTCG+AGG | - | Chr4:73199854-73199873 | MsG0480022492.01.T03:CDS | 45.0% |
| AAGAGGGACGGAACAACTTC+AGG | - | Chr4:73200571-73200590 | MsG0480022492.01.T02:CDS | 50.0% | |
| ! | GCAGCTTTGGTAGAGTCTCA+AGG | - | Chr4:73200494-73200513 | MsG0480022492.01.T03:CDS | 50.0% |
| !! | ACCTTGATGATGTCGAGGCA+GGG | - | Chr4:73199859-73199878 | MsG0480022492.01.T03:CDS | 50.0% |
| !! | TACCTTGATGATGTCGAGGC+AGG | - | Chr4:73199858-73199877 | MsG0480022492.01.T03:CDS | 50.0% |
| AACATTCCAGGGTGTGGTCC+AGG | - | Chr4:73200131-73200150 | MsG0480022492.01.T03:CDS | 55.0% | |
| TCCCTGCCTCGACATCATCA+AGG | + | Chr4:73199863-73199882 | None:intergenic | 55.0% | |
| GATGAACCTGGACCACACCC+TGG | + | Chr4:73200140-73200159 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 73199801 | 73200718 | 73199801 | ID=MsG0480022492.01;Name=MsG0480022492.01 |
| Chr4 | mRNA | 73199801 | 73200577 | 73199801 | ID=MsG0480022492.01.T01;Parent=MsG0480022492.01;Name=MsG0480022492.01.T01;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|0|0|2|0|248 |
| Chr4 | exon | 73199801 | 73200102 | 73199801 | ID=MsG0480022492.01.T01:exon:10280;Parent=MsG0480022492.01.T01 |
| Chr4 | exon | 73200133 | 73200577 | 73200133 | ID=MsG0480022492.01.T01:exon:10279;Parent=MsG0480022492.01.T01 |
| Chr4 | CDS | 73200133 | 73200577 | 73200133 | ID=MsG0480022492.01.T01:cds;Parent=MsG0480022492.01.T01 |
| Chr4 | CDS | 73199801 | 73200102 | 73199801 | ID=MsG0480022492.01.T01:cds;Parent=MsG0480022492.01.T01 |
| Chr4 | mRNA | 73199801 | 73200718 | 73199801 | ID=MsG0480022492.01.T02;Parent=MsG0480022492.01;Name=MsG0480022492.01.T02;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|305 |
| Chr4 | exon | 73199801 | 73200718 | 73199801 | ID=MsG0480022492.01.T02:exon:10281;Parent=MsG0480022492.01.T02 |
| Chr4 | CDS | 73199801 | 73200718 | 73199801 | ID=MsG0480022492.01.T02:cds;Parent=MsG0480022492.01.T02 |
| Chr4 | mRNA | 73199801 | 73200577 | 73199801 | ID=MsG0480022492.01.T03;Parent=MsG0480022492.01;Name=MsG0480022492.01.T03;_AED=0.08;_eAED=0.08;_QI=0|-1|0|1|-1|0|1|0|258 |
| Chr4 | exon | 73199801 | 73200577 | 73199801 | ID=MsG0480022492.01.T03:exon:10282;Parent=MsG0480022492.01.T03 |
| Chr4 | CDS | 73199801 | 73200577 | 73199801 | ID=MsG0480022492.01.T03:cds;Parent=MsG0480022492.01.T03 |
| Chr4 | mRNA | 73199801 | 73200028 | 73199801 | ID=MsG0480022492.01.T04;Parent=MsG0480022492.01;Name=MsG0480022492.01.T04;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|0|1|0|75 |
| Chr4 | exon | 73199801 | 73200028 | 73199801 | ID=MsG0480022492.01.T04:exon:10283;Parent=MsG0480022492.01.T04 |
| Chr4 | CDS | 73199801 | 73200028 | 73199801 | ID=MsG0480022492.01.T04:cds;Parent=MsG0480022492.01.T04 |
| Gene Sequence |
| Protein sequence |
