Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014501.01.T01 | XP_039687154.1 | 70.149 | 67 | 20 | 0 | 1 | 67 | 26 | 92 | 6.21E-20 | 93.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014501.01.T01 | A0A396IRT2 | 70.149 | 67 | 20 | 0 | 1 | 67 | 26 | 92 | 2.97e-20 | 93.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014501.01 | MsG0580028022.01 | 0.817362 | 3.430697e-52 | 2.493208e-49 |
| MsG0380014501.01 | MsG0880044179.01 | 0.814648 | 1.384493e-51 | 9.342700e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014501.01.T01 | MTR_3g051060 | 68.182 | 66 | 21 | 0 | 2 | 67 | 33 | 98 | 1.81e-22 | 89.7 |
| MsG0380014501.01.T01 | MTR_3g058550 | 69.565 | 46 | 14 | 0 | 22 | 67 | 1 | 46 | 1.21e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTAAACTTGGAAGGAAAT+TGG | 0.292076 | 3:+55722868 | None:intergenic |
| TTGAATCCTCTTGATGTTGA+AGG | 0.391526 | 3:-55723090 | None:intergenic |
| ATCTCTATTGTGCTAAACTT+AGG | 0.441348 | 3:-55722921 | None:intergenic |
| AGGAAATTGGAAAGAGGTTG+AGG | 0.479451 | 3:+55722881 | MsG0380014501.01.T01:CDS |
| GATAATAAACAACATTTACA+TGG | 0.517585 | 3:-55722989 | None:intergenic |
| CTTGGAAGGAAATTGGAAAG+AGG | 0.523946 | 3:+55722875 | MsG0380014501.01.T01:CDS |
| TTTGCACCTTCAACATCAAG+AGG | 0.529318 | 3:+55723084 | MsG0380014501.01.T01:CDS |
| AAATGTTGTTTATTATCTTG+TGG | 0.547211 | 3:+55722995 | MsG0380014501.01.T01:CDS |
| CCATGAATGTAAGAATGAAA+AGG | 0.548192 | 3:+55723052 | MsG0380014501.01.T01:CDS |
| ACATAGGAGCTAAACTTGGA+AGG | 0.589352 | 3:+55722861 | None:intergenic |
| TCTTGATGTTGAAGGTGCAA+AGG | 0.605288 | 3:-55723082 | None:intergenic |
| ATAGAGATCAACAAAAGTAG+AGG | 0.623346 | 3:+55722936 | MsG0380014501.01.T01:CDS |
| TAGAGGAACAGCACTACACG+TGG | 0.628712 | 3:+55722953 | MsG0380014501.01.T01:CDS |
| AGAGGAACAGCACTACACGT+GGG | 0.757144 | 3:+55722954 | MsG0380014501.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAATAAACAACATTTACA+TGG | - | Chr3:55722992-55723011 | None:intergenic | 20.0% |
| !!! | AAATGTTGTTTATTATCTTG+TGG | + | Chr3:55722995-55723014 | MsG0380014501.01.T01:CDS | 20.0% |
| !! | CTTTTCATTCTTACATTCAT+GGG | - | Chr3:55723054-55723073 | None:intergenic | 25.0% |
| ATAGAGATCAACAAAAGTAG+AGG | + | Chr3:55722936-55722955 | MsG0380014501.01.T01:CDS | 30.0% | |
| ATCTCTATTGTGCTAAACTT+AGG | - | Chr3:55722924-55722943 | None:intergenic | 30.0% | |
| CATACGTAACAAAAATGAGA+AGG | + | Chr3:55723025-55723044 | MsG0380014501.01.T01:CDS | 30.0% | |
| CCATGAATGTAAGAATGAAA+AGG | + | Chr3:55723052-55723071 | MsG0380014501.01.T01:CDS | 30.0% | |
| ! | CCTTTTCATTCTTACATTCA+TGG | - | Chr3:55723055-55723074 | None:intergenic | 30.0% |
| ACGTAACAAAAATGAGAAGG+AGG | + | Chr3:55723028-55723047 | MsG0380014501.01.T01:CDS | 35.0% | |
| TTGAATCCTCTTGATGTTGA+AGG | - | Chr3:55723093-55723112 | None:intergenic | 35.0% | |
| AGGAAATTGGAAAGAGGTTG+AGG | + | Chr3:55722881-55722900 | MsG0380014501.01.T01:CDS | 40.0% | |
| CTTGGAAGGAAATTGGAAAG+AGG | + | Chr3:55722875-55722894 | MsG0380014501.01.T01:CDS | 40.0% | |
| TTTGCACCTTCAACATCAAG+AGG | + | Chr3:55723084-55723103 | MsG0380014501.01.T01:CDS | 40.0% | |
| ! | TCTTGATGTTGAAGGTGCAA+AGG | - | Chr3:55723085-55723104 | None:intergenic | 40.0% |
| AGAGGAACAGCACTACACGT+GGG | + | Chr3:55722954-55722973 | MsG0380014501.01.T01:CDS | 50.0% | |
| TAGAGGAACAGCACTACACG+TGG | + | Chr3:55722953-55722972 | MsG0380014501.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 55722873 | 55723124 | 55722873 | ID=MsG0380014501.01;Name=MsG0380014501.01 |
| Chr3 | mRNA | 55722873 | 55723124 | 55722873 | ID=MsG0380014501.01.T01;Parent=MsG0380014501.01;Name=MsG0380014501.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|0|1|0|83 |
| Chr3 | exon | 55722873 | 55723124 | 55722873 | ID=MsG0380014501.01.T01:exon:23873;Parent=MsG0380014501.01.T01 |
| Chr3 | CDS | 55722873 | 55723124 | 55722873 | ID=MsG0380014501.01.T01:cds;Parent=MsG0380014501.01.T01 |
| Gene Sequence |
| Protein sequence |
