AlfalfaGEDB | MsG0380014612.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014612.01

KEGG: -
GO: GO:0003677
PFAM: PF02362

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014612.01.T01	XP_024634637.2	81.041	269	46	2	89	354	20	286	9.48E-154	444

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014612.01.T01	Q9XIB4	34.815	135	83	3	62	196	20	149	1.77E-14	74.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014612.01.T01	G8A2Z2	67.829	258	77	3	97	350	25	280	1.22e-116	348

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TFs/TRs:

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Gene ID	Type	Classification
MsG0380014612.01.T01	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000632.01	MsG0380014612.01	0.829506	4.956285e-55	5.082470e-52
MsG0180001200.01	MsG0380014612.01	0.805420	1.346842e-49	7.122091e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014612.01.T01	MTR_3g061370	67.829	258	77	3	97	350	25	280	3.08e-120	348
MsG0380014612.01.T01	MTR_3g061350	68.217	258	76	3	97	350	28	283	8.74e-120	347
MsG0380014612.01.T01	MTR_3g061340	69.380	258	63	5	97	350	28	273	2.20e-119	346
MsG0380014612.01.T01	MTR_3g061310	67.568	259	77	3	97	350	28	284	6.60e-119	345
MsG0380014612.01.T01	MTR_3g061320	68.235	255	76	2	97	350	28	278	3.32e-118	343
MsG0380014612.01.T01	MTR_3g061330	69.406	219	62	4	97	313	28	243	4.05e-102	301
MsG0380014612.01.T01	MTR_3g061290	80.420	143	28	0	89	231	20	162	1.09e-79	242
MsG0380014612.01.T01	MTR_3g061300	79.688	128	21	2	230	354	1	126	5.15e-65	202
MsG0380014612.01.T01	MTR_1g035460	33.333	288	148	10	99	350	6	285	2.74e-42	152
MsG0380014612.01.T01	MTR_3g061220	65.179	112	25	2	89	200	20	117	1.23e-41	142

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014612.01.T01	AT3G18990	25.234	321	175	8	89	348	16	332	7.34e-21	92.8
MsG0380014612.01.T01	AT1G49475	34.815	135	83	3	62	196	20	149	1.80e-15	74.3
MsG0380014612.01.T01	AT4G01580	30.709	127	81	2	89	214	40	160	6.52e-15	72.8
MsG0380014612.01.T01	AT3G18990	31.933	119	75	1	89	201	16	134	2.19e-14	73.9
MsG0380014612.01.T01	AT3G18960	33.696	92	61	0	90	181	41	132	1.80e-12	65.9
MsG0380014612.01.T01	AT3G18960	33.696	92	61	0	90	181	41	132	1.82e-12	66.2
MsG0380014612.01.T01	AT3G18960	36.145	83	53	0	99	181	13	95	2.17e-12	65.1

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Find 69 sgRNAs with CRISPR-Local

Find 309 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATACCAATGAGTTTACA+AGG	0.579007	3:+57192695	MsG0380014612.01.T01:CDS
AAAGAACTACATCATGGAAT+TGG	0.395138	3:+57194954	MsG0380014612.01.T01:CDS
AAGTATGTGATGCTGCAGAT+TGG	0.577298	3:+57195221	MsG0380014612.01.T01:CDS
AAGTTTGATGTATTAATACT+TGG	0.443758	3:+57192898	MsG0380014612.01.T01:CDS
AATGAGTTTACAAGGAGACA+TGG	0.642216	3:+57192703	MsG0380014612.01.T01:CDS
AATGGCGAAAATCTCGTTAA+TGG	0.355490	3:+57190890	MsG0380014612.01.T01:CDS
ACAAATGGATAAGGGCGACG+TGG	0.661171	3:-57190800	None:intergenic
ACATCATGGAATTGGCCAAA+GGG	0.541191	3:+57194962	MsG0380014612.01.T01:CDS
ACATTTCTAGCCTCACAATA+AGG	0.400255	3:-57195271	None:intergenic
ACCACTTTCTCTTGCAAACA+GGG	0.504654	3:-57195315	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGTCAATGGTGAGATT+TGG	+	Chr3:57192791-57192810	MsG0380014612.01.T01:CDS	30.0%
AAAACAAGCATTCAATCTCA+TGG	+	Chr3:57193756-57193775	MsG0380014612.01.T01:intron	30.0%
AAAACGAATCAAATAGTCTG+GGG	+	Chr3:57191120-57191139	MsG0380014612.01.T01:intron	30.0%
AAAGAACTACATCATGGAAT+TGG	+	Chr3:57194954-57194973	MsG0380014612.01.T01:CDS	30.0%
AAGAGAAGAAAAATGTGCTT+TGG	+	Chr3:57192174-57192193	MsG0380014612.01.T01:intron	30.0%
ACCTCTACTATAAAAAGTTC+AGG	+	Chr3:57193583-57193602	MsG0380014612.01.T01:intron	30.0%
AGAAAACGAATCAAATAGTC+TGG	+	Chr3:57191118-57191137	MsG0380014612.01.T01:intron	30.0%
AGAGAAGAAAAATGTGCTTT+GGG	+	Chr3:57192175-57192194	MsG0380014612.01.T01:intron	30.0%
AGAGGAAAAATTAAACCGAA+AGG	-	Chr3:57191633-57191652	None:intergenic	30.0%
AGTGTGTACCAACTTTAAAT+GGG	+	Chr3:57195180-57195199	MsG0380014612.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	57190764	57195424	57190764	ID=MsG0380014612.01;Name=MsG0380014612.01
Chr3	mRNA	57190764	57195424	57190764	ID=MsG0380014612.01.T01;Parent=MsG0380014612.01;Name=MsG0380014612.01.T01;_AED=0.63;_eAED=0.63;_QI=0\|0\|0\|0.6\|1\|1\|5\|0\|355
Chr3	exon	57190764	57190931	57190764	ID=MsG0380014612.01.T01:exon:16197;Parent=MsG0380014612.01.T01
Chr3	exon	57191397	57191508	57191397	ID=MsG0380014612.01.T01:exon:16198;Parent=MsG0380014612.01.T01
Chr3	exon	57192695	57193093	57192695	ID=MsG0380014612.01.T01:exon:16199;Parent=MsG0380014612.01.T01
Chr3	exon	57194933	57195078	57194933	ID=MsG0380014612.01.T01:exon:16200;Parent=MsG0380014612.01.T01
Chr3	exon	57195182	57195424	57195182	ID=MsG0380014612.01.T01:exon:16201;Parent=MsG0380014612.01.T01
Chr3	CDS	57190764	57190931	57190764	ID=MsG0380014612.01.T01:cds;Parent=MsG0380014612.01.T01
Chr3	CDS	57191397	57191508	57191397	ID=MsG0380014612.01.T01:cds;Parent=MsG0380014612.01.T01
Chr3	CDS	57192695	57193093	57192695	ID=MsG0380014612.01.T01:cds;Parent=MsG0380014612.01.T01
Chr3	CDS	57194933	57195078	57194933	ID=MsG0380014612.01.T01:cds;Parent=MsG0380014612.01.T01
Chr3	CDS	57195182	57195424	57195182	ID=MsG0380014612.01.T01:cds;Parent=MsG0380014612.01.T01

Gene Sequence

>MsG0380014612.01.T01

ATGCTGGATATCTGCTTATGCACCAATGAGGTGGCTCCACGTCGCCCTTATCCATTTGTCTTCTTGAAGTTCGCTGATGACTTCGCTAATGGCGAAACCTCTCTGGAACTTTGCCAACATTTCGCTAATGGCGAAAATCTCGTTAATGGCGAAAATCTTGTGAACTTGAAACAAATTTTTATTGAAAGGCGAATGAAGAAGATTTGTGAAGAAAACAAATTTTATGAAAGGCGAATGAAGAAGATTTGTGAACTTGGAATCGATAATGAGAAATTCTTAGAAAATACCAATGAGTTTACAAGGAGACATGGAGATAGTTTATCAAATCCAGTGTTGATCAAGAGTCCTGATGGTACTAAATGGAAAGTTTATTGGAAAAAAGTCAATGGTGAGATTTGGTTTGAAAAGGGTTGGAAATATTTTACTGAAAATTACTCTCTTGGACATGGCTGTTTGGTGGTGTTTGAATATAAAGGAACTTCAAAGTTTGATGTATTAATACTTGGCCAGAATGCAGTAGAAATAGACTATGATATTTCATGTGACACTGATGATGAAAATGACAATGTTGGTCATAGTGATGATGAATCAGTTGAGATTTTAGATGAATGGCAAAATCAGAAGAGTACTAGAAAAAAGCCGCCATTATTTTCTCCTCGACCACATAAGAAAGTAAGAGCTGTGGTAATGAAGACTAATCAAAGAACTACATCATGGAATTGGCCAAAGGGAGCTAGAGCTCAGGAAGTAACTTTAAAATTCGTCTCAAACAATCCTTTTTTCACAATTCTCATAAACCCTACTCATCTAGCAGAAAATCGGGTGTGTGTACCAACTTTAAATGGGGTTATTGAGAACAAAGAAAAGTATGTGATGCTGCAGATTGGGAAAAGATCAGGAATCTGGAAATGCTTCCTTATTGTGAGGCTAGAAATGTTCGCCGCTCTCGGCGGTTGGTCCCTGTTTGCAAGAGAAAGTGGTCTGCAAGTAGGAGATGTTTGTGTCTTTGAACTGATAAACAAGAATGATTTGGTTTTTAATGTTCATATTACCAAAAGTGACCGTTAA

Protein sequence

>MsG0380014612.01.T01

MLDICLCTNEVAPRRPYPFVFLKFADDFANGETSLELCQHFANGENLVNGENLVNLKQIFIERRMKKICEENKFYERRMKKICELGIDNEKFLENTNEFTRRHGDSLSNPVLIKSPDGTKWKVYWKKVNGEIWFEKGWKYFTENYSLGHGCLVVFEYKGTSKFDVLILGQNAVEIDYDISCDTDDENDNVGHSDDESVEILDEWQNQKSTRKKPPLFSPRPHKKVRAVVMKTNQRTTSWNWPKGARAQEVTLKFVSNNPFFTILINPTHLAENRVCVPTLNGVIENKEKYVMLQIGKRSGIWKCFLIVRLEMFAALGGWSLFARESGLQVGDVCVFELINKNDLVFNVHITKSDR*