Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001200.01.T01 | XP_039688129.1 | 93.86 | 114 | 6 | 1 | 4 | 117 | 11 | 123 | 6.16E-70 | 215 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048357.01 | MsG0180001200.01 | 0.877925 | 4.249722e-69 | 2.176822e-65 |
| MsG0180000632.01 | MsG0180001200.01 | 0.881312 | 2.665209e-70 | 1.553757e-66 |
| MsG0180000994.01 | MsG0180001200.01 | 0.825868 | 3.706423e-54 | 3.420230e-51 |
| MsG0180001200.01 | MsG0180001771.01 | 0.858375 | 8.601657e-63 | 2.190580e-59 |
| MsG0180001200.01 | MsG0180003431.01 | 0.845242 | 4.588386e-59 | 7.598605e-56 |
| MsG0180001200.01 | MsG0180003741.01 | 0.817142 | 3.844927e-52 | 2.777428e-49 |
| MsG0180001200.01 | MsG0180005540.01 | 0.814985 | 1.165773e-51 | 7.938496e-49 |
| MsG0180001200.01 | MsG0280007305.01 | 0.889789 | 1.767863e-73 | 1.446922e-69 |
| MsG0180001200.01 | MsG0280007414.01 | 0.825293 | 5.073463e-54 | 4.605405e-51 |
| MsG0180001200.01 | MsG0280008392.01 | 0.802168 | 6.376759e-49 | 3.102245e-46 |
| MsG0180001200.01 | MsG0280008395.01 | 0.806825 | 6.814575e-50 | 3.735694e-47 |
| MsG0180001200.01 | MsG0280010163.01 | 0.820072 | 8.318429e-53 | 6.516184e-50 |
| MsG0180001200.01 | MsG0280010320.01 | 0.816701 | 4.828445e-52 | 3.445977e-49 |
| MsG0180001200.01 | MsG0380011496.01 | 0.825330 | 4.972556e-54 | 4.518676e-51 |
| MsG0180001200.01 | MsG0380011501.01 | 0.814191 | 1.746747e-51 | 1.164233e-48 |
| MsG0180001200.01 | MsG0380011675.01 | 0.823616 | 1.258605e-53 | 1.089190e-50 |
| MsG0180001200.01 | MsG0380014612.01 | 0.805420 | 1.346842e-49 | 7.122091e-47 |
| MsG0180001200.01 | MsG0380016465.01 | 0.846410 | 2.211082e-59 | 3.802556e-56 |
| MsG0180001200.01 | MsG0380016466.01 | 0.838033 | 3.655799e-57 | 4.836811e-54 |
| MsG0180001200.01 | MsG0480018909.01 | 0.834641 | 2.665175e-56 | 3.181988e-53 |
| MsG0180001200.01 | MsG0480024022.01 | 0.832732 | 7.991570e-56 | 9.011081e-53 |
| MsG0180001200.01 | MsG0580024094.01 | 0.848453 | 6.070737e-60 | 1.114706e-56 |
| MsG0180001200.01 | MsG0580025974.01 | 0.801941 | 7.100836e-49 | 3.434453e-46 |
| MsG0180001200.01 | MsG0580026198.01 | 0.830663 | 2.586886e-55 | 2.743526e-52 |
| MsG0180001200.01 | MsG0580027925.01 | 0.800749 | 1.245475e-48 | 5.845109e-46 |
| MsG0180001200.01 | MsG0580029310.01 | 0.824393 | 8.272750e-54 | 7.317675e-51 |
| MsG0180001200.01 | MsG0580029405.01 | 0.829016 | 6.516538e-55 | 6.586719e-52 |
| MsG0180001200.01 | MsG0580029406.01 | 0.805821 | 1.109497e-49 | 5.927974e-47 |
| MsG0180001200.01 | MsG0580029417.01 | 0.878901 | 1.929557e-69 | 1.024945e-65 |
| MsG0180001200.01 | MsG0580029418.01 | 0.890678 | 7.927738e-74 | 6.734426e-70 |
| MsG0180001200.01 | MsG0680031342.01 | 0.811433 | 7.017452e-51 | 4.343409e-48 |
| MsG0180001200.01 | MsG0680031428.01 | 0.805135 | 1.544842e-49 | 8.109833e-47 |
| MsG0180001200.01 | MsG0680031707.01 | 0.890326 | 1.090336e-73 | 9.125991e-70 |
| MsG0180001200.01 | MsG0680033143.01 | 0.869735 | 2.478411e-66 | 9.398891e-63 |
| MsG0180001200.01 | MsG0680034835.01 | 0.810426 | 1.159821e-50 | 6.988669e-48 |
| MsG0180001200.01 | MsG0680035683.01 | 0.845814 | 3.210379e-59 | 5.416166e-56 |
| MsG0180001200.01 | MsG0780036148.01 | 0.863345 | 2.659763e-64 | 8.038105e-61 |
| MsG0180001200.01 | MsG0780036178.01 | 0.819695 | 1.014526e-52 | 7.863292e-50 |
| MsG0180001200.01 | MsG0780040846.01 | 0.840921 | 6.496528e-58 | 9.393710e-55 |
| MsG0180001200.01 | MsG0780040966.01 | 0.865328 | 6.395637e-65 | 2.072594e-61 |
| MsG0180001200.01 | MsG0780041676.01 | 0.820209 | 7.739466e-53 | 6.085222e-50 |
| MsG0180001200.01 | MsG0880041858.01 | 0.874614 | 5.887520e-68 | 2.667679e-64 |
| MsG0180001200.01 | MsG0880042442.01 | 0.815097 | 1.100448e-51 | 7.516089e-49 |
| MsG0180001200.01 | MsG0880045585.01 | 0.840134 | 1.043983e-57 | 1.473238e-54 |
| MsG0180001200.01 | MsG0880045586.01 | 0.835373 | 1.742458e-56 | 2.126709e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001200.01.T01 | MTR_1g032750 | 93.860 | 114 | 6 | 1 | 4 | 117 | 11 | 123 | 7.46e-74 | 215 |
| MsG0180001200.01.T01 | MTR_6g071190 | 40.000 | 115 | 64 | 2 | 1 | 115 | 28 | 137 | 9.72e-22 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001200.01.T01 | AT1G14760 | 42.157 | 102 | 55 | 3 | 14 | 112 | 32 | 132 | 8.56e-20 | 79.0 |
| MsG0180001200.01.T01 | AT1G70510 | 35.849 | 106 | 61 | 2 | 14 | 115 | 68 | 170 | 4.98e-11 | 58.5 |
Find 16 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTGGACTAGTTGTTGTTT+TGG | 0.335695 | 1:+17266915 | None:intergenic |
| CCATCCTTTGTTTGAAGCAT+TGG | 0.359729 | 1:-17267512 | MsG0180001200.01.T01:CDS |
| TGATCAAGCTCTGAATTGTT+TGG | 0.360004 | 1:+17266898 | None:intergenic |
| TTCAGAGCTTGATCACTTCA+TGG | 0.367274 | 1:-17266890 | MsG0180001200.01.T01:intron |
| AAGTGATGCATGGAAGAAAT+TGG | 0.438853 | 1:-17266950 | MsG0180001200.01.T01:CDS |
| GTCTCACATCAATTGTTTGA+AGG | 0.470627 | 1:-17267485 | MsG0180001200.01.T01:CDS |
| TATATTCAACTGCAGGAACT+TGG | 0.501461 | 1:-17265629 | MsG0180001200.01.T01:CDS |
| GTTCATCAATCACGTCTTTG+AGG | 0.505826 | 1:+17265687 | None:intergenic |
| AAAAGAAAGTGAAAATGAAG+AGG | 0.568561 | 1:-17267557 | MsG0180001200.01.T01:CDS |
| CCAATGCTTCAAACAAAGGA+TGG | 0.572089 | 1:+17267512 | None:intergenic |
| TTGATATAACAAGTGATGCA+TGG | 0.576358 | 1:-17266960 | MsG0180001200.01.T01:CDS |
| GGAAAGGGAAGTGATGATGT+AGG | 0.576930 | 1:-17267580 | None:intergenic |
| TGCTACGTATATTCAACTGC+AGG | 0.578517 | 1:-17265636 | MsG0180001200.01.T01:CDS |
| TCAACCAATGCTTCAAACAA+AGG | 0.579326 | 1:+17267508 | None:intergenic |
| TATTTCTTAGGTCGGATCTG+AGG | 0.611391 | 1:-17266995 | MsG0180001200.01.T01:intron |
| CAACTGCAGGAACTTGGTGA+AGG | 0.630993 | 1:-17265623 | MsG0180001200.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATAATCTATAAAAGA+TGG | + | Chr1:17267072-17267091 | None:intergenic | 10.0% |
| !! | AATAATATAGTGTATAATTA+TGG | - | Chr1:17265849-17265868 | MsG0180001200.01.T01:intron | 10.0% |
| !! | ATAGATATATAAATTCTTTA+CGG | - | Chr1:17266502-17266521 | MsG0180001200.01.T01:intron | 10.0% |
| !! | ATATTAATATATACACAAAA+AGG | - | Chr1:17265926-17265945 | MsG0180001200.01.T01:intron | 10.0% |
| !! | TATTAATATATACACAAAAA+GGG | - | Chr1:17265927-17265946 | MsG0180001200.01.T01:intron | 10.0% |
| !! | ACAACTTAAATTTATTTAAG+AGG | - | Chr1:17265748-17265767 | MsG0180001200.01.T01:intron | 15.0% |
| !! | GTATTACATACATTAATTAA+TGG | - | Chr1:17266319-17266338 | MsG0180001200.01.T01:intron | 15.0% |
| !!! | TTACTAAGTAACAATTTTTA+TGG | - | Chr1:17266545-17266564 | MsG0180001200.01.T01:intron | 15.0% |
| !!! | TTGTTACTTAGTAATTAATT+TGG | + | Chr1:17266539-17266558 | None:intergenic | 15.0% |
| !! | AAAATAATAGAGAGAGAAAA+AGG | + | Chr1:17266672-17266691 | None:intergenic | 20.0% |
| !! | AATCATATTATATGTGAACT+AGG | + | Chr1:17267439-17267458 | None:intergenic | 20.0% |
| !! | TAATTATGGAAAGATAGTTT+AGG | - | Chr1:17265863-17265882 | MsG0180001200.01.T01:intron | 20.0% |
| !!! | AAGAAAAATGGATTAATCAA+AGG | - | Chr1:17266120-17266139 | MsG0180001200.01.T01:intron | 20.0% |
| !!! | AGTAATTAATTTGGATGTTT+TGG | + | Chr1:17266530-17266549 | None:intergenic | 20.0% |
| !!! | TCTTTTTTGCAAAAGAAAAA+TGG | - | Chr1:17266108-17266127 | MsG0180001200.01.T01:intron | 20.0% |
| ! | AAAAGAAAGTGAAAATGAAG+AGG | - | Chr1:17265630-17265649 | MsG0180001200.01.T01:CDS | 25.0% |
| ! | CATACATTAATTAATGGTCA+TGG | - | Chr1:17266325-17266344 | MsG0180001200.01.T01:intron | 25.0% |
| ! | GTAACTAAAACTTGTCATTA+TGG | + | Chr1:17266819-17266838 | None:intergenic | 25.0% |
| ! | TAATTTCATTCCATACATGT+TGG | + | Chr1:17266152-17266171 | None:intergenic | 25.0% |
| ! | TATGATTCATTTGTGTACAT+AGG | - | Chr1:17267452-17267471 | MsG0180001200.01.T01:intron | 25.0% |
| ! | TCATGAAAGTTGTCTATTAA+TGG | - | Chr1:17266723-17266742 | MsG0180001200.01.T01:intron | 25.0% |
| ! | TGTGTGTAAAATAGATATTG+TGG | - | Chr1:17266458-17266477 | MsG0180001200.01.T01:intron | 25.0% |
| !! | AATCAAATTTGTTCACTCAA+TGG | - | Chr1:17266410-17266429 | MsG0180001200.01.T01:intron | 25.0% |
| !! | TCTTCATTCCTTTATTTCTT+AGG | - | Chr1:17266180-17266199 | MsG0180001200.01.T01:intron | 25.0% |
| !!! | ACGTAAATTGAAAGTTTTGA+AGG | + | Chr1:17266794-17266813 | None:intergenic | 25.0% |
| !!! | TTTTTAAGCAAGCATAAACT+AGG | - | Chr1:17267333-17267352 | MsG0180001200.01.T01:intron | 25.0% |
| AAGGATATATCCAACATGTA+TGG | - | Chr1:17266139-17266158 | MsG0180001200.01.T01:intron | 30.0% | |
| AGGGTAAAAAAGACAACAAA+AGG | - | Chr1:17265946-17265965 | MsG0180001200.01.T01:intron | 30.0% | |
| CTTGGTGAAGGAAATAATTA+AGG | - | Chr1:17267576-17267595 | MsG0180001200.01.T01:CDS | 30.0% | |
| GGGTAAAAAAGACAACAAAA+GGG | - | Chr1:17265947-17265966 | MsG0180001200.01.T01:intron | 30.0% | |
| GTCAAAAAGTTGTTGCAAAA+TGG | + | Chr1:17266605-17266624 | None:intergenic | 30.0% | |
| TGTAAGTAATGTATTCCTCA+TGG | + | Chr1:17265899-17265918 | None:intergenic | 30.0% | |
| TTGATATAACAAGTGATGCA+TGG | - | Chr1:17266227-17266246 | MsG0180001200.01.T01:intron | 30.0% | |
| ! | AAAGCTTTTGCTTCTTTTGT+TGG | + | Chr1:17267525-17267544 | None:intergenic | 30.0% |
| ! | CATTCCTTTATTTCTTAGGT+CGG | - | Chr1:17266184-17266203 | MsG0180001200.01.T01:intron | 30.0% |
| AAGTGATGCATGGAAGAAAT+TGG | - | Chr1:17266237-17266256 | MsG0180001200.01.T01:intron | 35.0% | |
| AGATCCGACCTAAGAAATAA+AGG | + | Chr1:17266191-17266210 | None:intergenic | 35.0% | |
| GTCTCACATCAATTGTTTGA+AGG | - | Chr1:17265702-17265721 | MsG0180001200.01.T01:CDS | 35.0% | |
| TATATTCAACTGCAGGAACT+TGG | - | Chr1:17267558-17267577 | MsG0180001200.01.T01:CDS | 35.0% | |
| TCAACCAATGCTTCAAACAA+AGG | + | Chr1:17265682-17265701 | None:intergenic | 35.0% | |
| !! | TGATCAAGCTCTGAATTGTT+TGG | + | Chr1:17266292-17266311 | None:intergenic | 35.0% |
| !!! | GTTTGGACTAGTTGTTGTTT+TGG | + | Chr1:17266275-17266294 | None:intergenic | 35.0% |
| !!! | TACATAGGAAGCATTTTGCA+TGG | - | Chr1:17267467-17267486 | MsG0180001200.01.T01:intron | 35.0% |
| AATACGTTGTGACGTACGTA+TGG | + | Chr1:17266870-17266889 | None:intergenic | 40.0% | |
| CCAATGCTTCAAACAAAGGA+TGG | + | Chr1:17265678-17265697 | None:intergenic | 40.0% | |
| GTTCATCAATCACGTCTTTG+AGG | + | Chr1:17267503-17267522 | None:intergenic | 40.0% | |
| TATTTCTTAGGTCGGATCTG+AGG | - | Chr1:17266192-17266211 | MsG0180001200.01.T01:intron | 40.0% | |
| TGCTACGTATATTCAACTGC+AGG | - | Chr1:17267551-17267570 | MsG0180001200.01.T01:CDS | 40.0% | |
| TTCAGAGCTTGATCACTTCA+TGG | - | Chr1:17266297-17266316 | MsG0180001200.01.T01:intron | 40.0% | |
| ! | CCATCCTTTGTTTGAAGCAT+TGG | - | Chr1:17265675-17265694 | MsG0180001200.01.T01:CDS | 40.0% |
| ! | GGAAAGATAGTTTAGGACCA+CGG | - | Chr1:17265870-17265889 | MsG0180001200.01.T01:intron | 40.0% |
| !! | GTACGTATGGTTTTGCATGT+GGG | + | Chr1:17266857-17266876 | None:intergenic | 40.0% |
| !! | ATGGTTTTGCATGTGGGTTG+AGG | + | Chr1:17266851-17266870 | None:intergenic | 45.0% |
| !! | CGTACGTATGGTTTTGCATG+TGG | + | Chr1:17266858-17266877 | None:intergenic | 45.0% |
| !! | GACGTACGTATGGTTTTGCT+AGG | + | Chr1:17267121-17267140 | None:intergenic | 45.0% |
| !! | TGGTTTTGCATGTGGGTTGA+GGG | + | Chr1:17266850-17266869 | None:intergenic | 45.0% |
| TGTATTCCTCATGGTGACCG+TGG | + | Chr1:17265890-17265909 | None:intergenic | 50.0% | |
| ! | CAACTGCAGGAACTTGGTGA+AGG | - | Chr1:17267564-17267583 | MsG0180001200.01.T01:CDS | 50.0% |
| ! | TTGCATGTGGGTTGAGGGTT+CGG | + | Chr1:17266845-17266864 | None:intergenic | 50.0% |
| TTAGGACCACGGTCACCATG+AGG | - | Chr1:17265881-17265900 | MsG0180001200.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 17265613 | 17267596 | 17265613 | ID=MsG0180001200.01;Name=MsG0180001200.01 |
| Chr1 | mRNA | 17265613 | 17267596 | 17265613 | ID=MsG0180001200.01.T01;Parent=MsG0180001200.01;Name=MsG0180001200.01.T01;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|0|0|3|0|117 |
| Chr1 | exon | 17267483 | 17267596 | 17267483 | ID=MsG0180001200.01.T01:exon:17455;Parent=MsG0180001200.01.T01 |
| Chr1 | exon | 17266891 | 17267007 | 17266891 | ID=MsG0180001200.01.T01:exon:17456;Parent=MsG0180001200.01.T01 |
| Chr1 | exon | 17265613 | 17265735 | 17265613 | ID=MsG0180001200.01.T01:exon:17457;Parent=MsG0180001200.01.T01 |
| Chr1 | CDS | 17267483 | 17267596 | 17267483 | ID=MsG0180001200.01.T01:cds;Parent=MsG0180001200.01.T01 |
| Chr1 | CDS | 17266891 | 17267007 | 17266891 | ID=MsG0180001200.01.T01:cds;Parent=MsG0180001200.01.T01 |
| Chr1 | CDS | 17265613 | 17265735 | 17265613 | ID=MsG0180001200.01.T01:cds;Parent=MsG0180001200.01.T01 |
| Gene Sequence |
| Protein sequence |
