Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014910.01.T01 | XP_014505133.1 | 78.607 | 201 | 15 | 1 | 20 | 192 | 134 | 334 | 1.09E-106 | 322 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014910.01.T01 | Q8RX37 | 58.621 | 203 | 54 | 3 | 20 | 192 | 125 | 327 | 1.94E-75 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014910.01.T01 | A0A072UFF5 | 83.857 | 223 | 8 | 1 | 20 | 214 | 126 | 348 | 5.67e-127 | 372 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005172.01 | MsG0380014910.01 | 0.834232 | 3.376874e-56 | 3.982431e-53 |
| MsG0380014910.01 | MsG0580029259.01 | 0.911598 | 5.070948e-83 | 1.094577e-78 |
| MsG0380014910.01 | MsG0780037727.01 | 0.800271 | 1.558632e-48 | 7.226755e-46 |
| MsG0280010459.01 | MsG0380014910.01 | 0.804934 | 1.701777e-49 | 8.888070e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014910.01.T01 | MTR_5g071550 | 83.857 | 223 | 8 | 1 | 20 | 214 | 126 | 348 | 1.44e-130 | 372 |
| MsG0380014910.01.T01 | MTR_5g071550 | 82.297 | 209 | 9 | 1 | 20 | 200 | 126 | 334 | 3.38e-120 | 346 |
| MsG0380014910.01.T01 | MTR_3g464650 | 63.636 | 209 | 48 | 1 | 20 | 200 | 66 | 274 | 2.38e-92 | 273 |
| MsG0380014910.01.T01 | MTR_3g464700 | 65.174 | 201 | 42 | 1 | 20 | 192 | 90 | 290 | 2.53e-92 | 274 |
| MsG0380014910.01.T01 | MTR_5g071550 | 75.342 | 146 | 8 | 1 | 20 | 137 | 126 | 271 | 3.58e-71 | 218 |
| MsG0380014910.01.T01 | MTR_1g014640 | 37.019 | 208 | 96 | 3 | 25 | 200 | 85 | 289 | 1.87e-35 | 128 |
| MsG0380014910.01.T01 | MTR_1g013400 | 37.019 | 208 | 96 | 3 | 25 | 200 | 85 | 289 | 1.87e-35 | 128 |
| MsG0380014910.01.T01 | MTR_3g105730 | 36.500 | 200 | 92 | 3 | 25 | 192 | 20 | 216 | 2.45e-33 | 121 |
| MsG0380014910.01.T01 | MTR_3g105730 | 36.500 | 200 | 92 | 3 | 25 | 192 | 42 | 238 | 4.46e-33 | 120 |
| MsG0380014910.01.T01 | MTR_3g091060 | 35.644 | 202 | 95 | 3 | 24 | 193 | 101 | 299 | 2.41e-32 | 120 |
| MsG0380014910.01.T01 | MTR_1g115570 | 36.816 | 201 | 88 | 6 | 24 | 192 | 102 | 295 | 5.47e-32 | 119 |
| MsG0380014910.01.T01 | MTR_4g125810 | 35.135 | 185 | 76 | 4 | 48 | 190 | 253 | 435 | 1.64e-23 | 97.8 |
| MsG0380014910.01.T01 | MTR_4g116420 | 32.735 | 223 | 86 | 8 | 25 | 193 | 93 | 305 | 1.96e-23 | 96.7 |
| MsG0380014910.01.T01 | MTR_3g068200 | 31.628 | 215 | 98 | 4 | 25 | 192 | 100 | 312 | 1.65e-21 | 91.7 |
| MsG0380014910.01.T01 | MTR_8g463130 | 34.715 | 193 | 89 | 4 | 30 | 189 | 20 | 208 | 2.35e-21 | 89.0 |
| MsG0380014910.01.T01 | MTR_5g080680 | 31.455 | 213 | 101 | 3 | 25 | 193 | 109 | 320 | 2.57e-21 | 90.9 |
| MsG0380014910.01.T01 | MTR_8g463130 | 34.715 | 193 | 89 | 4 | 30 | 189 | 48 | 236 | 4.12e-21 | 89.0 |
| MsG0380014910.01.T01 | MTR_2g078760 | 33.500 | 200 | 95 | 5 | 24 | 189 | 13 | 208 | 1.13e-20 | 87.4 |
| MsG0380014910.01.T01 | MTR_2g078760 | 33.500 | 200 | 95 | 5 | 24 | 189 | 92 | 287 | 1.26e-20 | 87.8 |
| MsG0380014910.01.T01 | MTR_2g078760 | 33.500 | 200 | 95 | 5 | 24 | 189 | 92 | 287 | 2.46e-20 | 87.8 |
| MsG0380014910.01.T01 | MTR_1g050520 | 30.631 | 222 | 109 | 4 | 21 | 200 | 391 | 609 | 2.82e-20 | 89.0 |
| MsG0380014910.01.T01 | MTR_1g028300 | 34.500 | 200 | 95 | 4 | 25 | 189 | 120 | 318 | 5.47e-20 | 87.4 |
| MsG0380014910.01.T01 | MTR_4g094542 | 35.028 | 177 | 79 | 6 | 45 | 189 | 147 | 319 | 2.75e-19 | 85.1 |
| MsG0380014910.01.T01 | MTR_5g063940 | 31.905 | 210 | 104 | 7 | 14 | 189 | 32 | 236 | 2.97e-19 | 84.0 |
| MsG0380014910.01.T01 | MTR_5g063940 | 32.990 | 194 | 91 | 7 | 30 | 189 | 1 | 189 | 3.83e-19 | 82.8 |
| MsG0380014910.01.T01 | MTR_1g015110 | 31.429 | 210 | 89 | 5 | 32 | 190 | 273 | 478 | 4.11e-19 | 85.5 |
| MsG0380014910.01.T01 | MTR_8g102550 | 36.667 | 180 | 76 | 5 | 48 | 190 | 152 | 330 | 5.85e-19 | 84.3 |
| MsG0380014910.01.T01 | MTR_3g104710 | 30.808 | 198 | 83 | 4 | 43 | 190 | 281 | 474 | 1.11e-18 | 84.3 |
| MsG0380014910.01.T01 | MTR_3g074610 | 30.374 | 214 | 112 | 6 | 9 | 189 | 27 | 236 | 4.34e-18 | 80.9 |
| MsG0380014910.01.T01 | MTR_2g033000 | 29.146 | 199 | 92 | 4 | 37 | 190 | 89 | 283 | 5.59e-18 | 81.6 |
| MsG0380014910.01.T01 | MTR_2g040500 | 48.837 | 86 | 43 | 1 | 107 | 192 | 388 | 472 | 9.47e-18 | 81.3 |
| MsG0380014910.01.T01 | MTR_2g008850 | 29.730 | 222 | 121 | 7 | 16 | 204 | 31 | 250 | 1.52e-17 | 79.3 |
| MsG0380014910.01.T01 | MTR_2g008850 | 30.583 | 206 | 110 | 5 | 30 | 204 | 1 | 204 | 3.10e-17 | 77.8 |
| MsG0380014910.01.T01 | MTR_4g094208 | 29.858 | 211 | 111 | 9 | 13 | 189 | 24 | 231 | 5.88e-17 | 77.8 |
| MsG0380014910.01.T01 | MTR_5g009370 | 30.986 | 213 | 99 | 5 | 25 | 192 | 60 | 269 | 6.58e-17 | 78.2 |
| MsG0380014910.01.T01 | MTR_4g007440 | 35.000 | 100 | 52 | 2 | 108 | 198 | 189 | 284 | 4.27e-12 | 64.7 |
| MsG0380014910.01.T01 | MTR_3g105880 | 32.174 | 115 | 64 | 3 | 108 | 213 | 189 | 298 | 4.74e-11 | 61.6 |
| MsG0380014910.01.T01 | MTR_3g105880 | 34.000 | 100 | 53 | 2 | 108 | 198 | 189 | 284 | 5.39e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014910.01.T01 | AT1G07160 | 58.621 | 203 | 54 | 3 | 20 | 192 | 125 | 327 | 1.98e-76 | 235 |
| MsG0380014910.01.T01 | AT2G30020 | 59.606 | 203 | 52 | 3 | 20 | 192 | 142 | 344 | 6.27e-76 | 234 |
| MsG0380014910.01.T01 | AT2G40180 | 55.981 | 209 | 54 | 5 | 20 | 192 | 131 | 337 | 1.41e-66 | 210 |
| MsG0380014910.01.T01 | AT1G67820 | 44.651 | 215 | 88 | 3 | 20 | 204 | 87 | 300 | 1.30e-52 | 174 |
| MsG0380014910.01.T01 | AT1G67820 | 44.651 | 215 | 88 | 3 | 20 | 204 | 123 | 336 | 3.07e-52 | 174 |
| MsG0380014910.01.T01 | AT2G05050 | 45.732 | 164 | 65 | 3 | 30 | 192 | 1 | 141 | 2.03e-37 | 129 |
| MsG0380014910.01.T01 | AT4G08260 | 43.820 | 178 | 67 | 5 | 30 | 192 | 1 | 160 | 4.43e-37 | 129 |
| MsG0380014910.01.T01 | AT4G31750 | 38.000 | 200 | 89 | 3 | 25 | 192 | 41 | 237 | 6.18e-34 | 123 |
| MsG0380014910.01.T01 | AT4G31750 | 38.000 | 200 | 89 | 3 | 25 | 192 | 41 | 237 | 6.49e-34 | 123 |
| MsG0380014910.01.T01 | AT4G31750 | 38.000 | 200 | 89 | 3 | 25 | 192 | 41 | 237 | 6.49e-34 | 123 |
| MsG0380014910.01.T01 | AT5G10740 | 36.869 | 198 | 90 | 3 | 25 | 190 | 41 | 235 | 1.90e-32 | 120 |
| MsG0380014910.01.T01 | AT5G10740 | 36.869 | 198 | 90 | 3 | 25 | 190 | 41 | 235 | 1.90e-32 | 120 |
| MsG0380014910.01.T01 | AT5G53140 | 36.098 | 205 | 95 | 4 | 21 | 192 | 104 | 305 | 2.09e-32 | 121 |
| MsG0380014910.01.T01 | AT5G53140 | 36.098 | 205 | 95 | 4 | 21 | 192 | 104 | 305 | 2.09e-32 | 121 |
| MsG0380014910.01.T01 | AT5G24940 | 35.096 | 208 | 100 | 3 | 25 | 200 | 41 | 245 | 3.04e-31 | 119 |
| MsG0380014910.01.T01 | AT3G27140 | 40.719 | 167 | 68 | 3 | 30 | 192 | 1 | 140 | 1.71e-30 | 112 |
| MsG0380014910.01.T01 | AT1G43900 | 35.071 | 211 | 97 | 6 | 24 | 200 | 130 | 334 | 6.67e-30 | 114 |
| MsG0380014910.01.T01 | AT4G28400 | 29.358 | 218 | 116 | 5 | 21 | 204 | 39 | 252 | 2.43e-22 | 91.7 |
| MsG0380014910.01.T01 | AT4G26080 | 32.719 | 217 | 94 | 6 | 25 | 190 | 136 | 351 | 1.01e-21 | 92.4 |
| MsG0380014910.01.T01 | AT5G57050 | 33.333 | 204 | 87 | 5 | 30 | 190 | 104 | 301 | 1.08e-21 | 92.0 |
| MsG0380014910.01.T01 | AT5G57050 | 34.409 | 186 | 75 | 4 | 48 | 190 | 160 | 341 | 1.67e-21 | 91.7 |
| MsG0380014910.01.T01 | AT3G15260 | 34.715 | 193 | 89 | 5 | 30 | 189 | 55 | 243 | 2.81e-21 | 89.7 |
| MsG0380014910.01.T01 | AT3G15260 | 34.715 | 193 | 89 | 5 | 30 | 189 | 55 | 243 | 2.81e-21 | 89.7 |
| MsG0380014910.01.T01 | AT1G78200 | 32.821 | 195 | 91 | 5 | 30 | 188 | 1 | 191 | 3.14e-21 | 88.6 |
| MsG0380014910.01.T01 | AT1G78200 | 32.821 | 195 | 91 | 5 | 30 | 188 | 1 | 191 | 3.14e-21 | 88.6 |
| MsG0380014910.01.T01 | AT4G28400 | 30.542 | 203 | 103 | 5 | 21 | 189 | 17 | 215 | 4.04e-21 | 88.6 |
| MsG0380014910.01.T01 | AT1G78200 | 32.039 | 206 | 100 | 5 | 19 | 188 | 35 | 236 | 5.28e-21 | 88.6 |
| MsG0380014910.01.T01 | AT1G78200 | 32.039 | 206 | 100 | 5 | 19 | 188 | 35 | 236 | 5.28e-21 | 88.6 |
| MsG0380014910.01.T01 | AT1G78200 | 32.039 | 206 | 100 | 5 | 19 | 188 | 35 | 236 | 5.28e-21 | 88.6 |
| MsG0380014910.01.T01 | AT4G28400 | 30.542 | 203 | 103 | 5 | 21 | 189 | 39 | 237 | 7.19e-21 | 88.6 |
| MsG0380014910.01.T01 | AT2G29380 | 32.420 | 219 | 95 | 6 | 25 | 192 | 85 | 301 | 2.22e-20 | 88.2 |
| MsG0380014910.01.T01 | AT2G40860 | 30.000 | 230 | 111 | 5 | 17 | 200 | 388 | 613 | 4.67e-20 | 88.2 |
| MsG0380014910.01.T01 | AT2G40860 | 30.455 | 220 | 103 | 5 | 17 | 190 | 388 | 603 | 4.91e-20 | 88.2 |
| MsG0380014910.01.T01 | AT2G40860 | 30.000 | 230 | 111 | 5 | 17 | 200 | 290 | 515 | 5.90e-20 | 87.8 |
| MsG0380014910.01.T01 | AT1G07430 | 29.680 | 219 | 102 | 4 | 25 | 192 | 128 | 345 | 9.63e-20 | 87.0 |
| MsG0380014910.01.T01 | AT2G20630 | 30.392 | 204 | 103 | 5 | 19 | 189 | 36 | 233 | 1.98e-19 | 84.3 |
| MsG0380014910.01.T01 | AT3G11410 | 30.942 | 223 | 98 | 3 | 25 | 192 | 112 | 333 | 2.39e-19 | 85.5 |
| MsG0380014910.01.T01 | AT2G20630 | 30.392 | 204 | 103 | 5 | 19 | 189 | 36 | 233 | 3.01e-19 | 84.0 |
| MsG0380014910.01.T01 | AT5G51760 | 50.575 | 87 | 42 | 1 | 104 | 190 | 226 | 311 | 5.01e-18 | 81.6 |
| MsG0380014910.01.T01 | AT5G51760 | 50.575 | 87 | 42 | 1 | 104 | 190 | 256 | 341 | 6.32e-18 | 81.6 |
| MsG0380014910.01.T01 | AT2G34740 | 30.102 | 196 | 94 | 6 | 30 | 189 | 97 | 285 | 7.18e-18 | 80.5 |
| MsG0380014910.01.T01 | AT2G34740 | 30.102 | 196 | 94 | 6 | 30 | 189 | 115 | 303 | 7.19e-18 | 80.9 |
| MsG0380014910.01.T01 | AT1G22280 | 29.048 | 210 | 106 | 8 | 19 | 189 | 36 | 241 | 9.27e-18 | 80.1 |
| MsG0380014910.01.T01 | AT2G34740 | 30.102 | 196 | 94 | 6 | 30 | 189 | 101 | 289 | 1.31e-17 | 80.1 |
| MsG0380014910.01.T01 | AT5G59220 | 30.213 | 235 | 105 | 5 | 14 | 192 | 102 | 333 | 1.35e-17 | 80.9 |
| MsG0380014910.01.T01 | AT1G22280 | 29.557 | 203 | 108 | 7 | 19 | 189 | 36 | 235 | 4.72e-17 | 78.2 |
| MsG0380014910.01.T01 | AT1G72770 | 51.724 | 87 | 41 | 1 | 104 | 190 | 351 | 436 | 1.37e-16 | 78.2 |
| MsG0380014910.01.T01 | AT1G72770 | 51.724 | 87 | 41 | 1 | 104 | 190 | 351 | 436 | 1.37e-16 | 78.2 |
| MsG0380014910.01.T01 | AT1G72770 | 51.724 | 87 | 41 | 1 | 104 | 190 | 351 | 436 | 1.37e-16 | 78.2 |
| MsG0380014910.01.T01 | AT1G34750 | 31.414 | 191 | 98 | 5 | 30 | 189 | 48 | 236 | 2.92e-15 | 72.4 |
| MsG0380014910.01.T01 | AT1G34750 | 31.414 | 191 | 98 | 5 | 30 | 189 | 48 | 236 | 3.57e-15 | 72.8 |
| MsG0380014910.01.T01 | AT1G34750 | 31.414 | 191 | 98 | 5 | 30 | 189 | 48 | 236 | 3.57e-15 | 72.8 |
| MsG0380014910.01.T01 | AT1G34750 | 31.414 | 191 | 98 | 5 | 30 | 189 | 48 | 236 | 3.57e-15 | 72.8 |
| MsG0380014910.01.T01 | AT1G17550 | 50.000 | 78 | 38 | 1 | 113 | 190 | 360 | 436 | 1.32e-14 | 72.4 |
| MsG0380014910.01.T01 | AT1G17550 | 50.000 | 78 | 38 | 1 | 113 | 190 | 360 | 436 | 1.32e-14 | 72.4 |
| MsG0380014910.01.T01 | AT3G63340 | 41.837 | 98 | 47 | 4 | 105 | 194 | 298 | 393 | 2.03e-13 | 68.9 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 298 | 395 | 2.12e-13 | 68.9 |
| MsG0380014910.01.T01 | AT4G33920 | 25.571 | 219 | 92 | 5 | 43 | 190 | 56 | 274 | 2.81e-13 | 68.2 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 331 | 428 | 2.90e-13 | 68.6 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 298 | 395 | 3.04e-13 | 68.6 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 298 | 395 | 3.14e-13 | 68.6 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 298 | 395 | 3.14e-13 | 68.6 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 298 | 395 | 3.16e-13 | 68.6 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 298 | 395 | 3.16e-13 | 68.6 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 298 | 395 | 3.16e-13 | 68.6 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 298 | 395 | 3.16e-13 | 68.6 |
| MsG0380014910.01.T01 | AT3G63340 | 41.000 | 100 | 49 | 4 | 105 | 196 | 298 | 395 | 3.18e-13 | 68.6 |
| MsG0380014910.01.T01 | AT3G63320 | 41.111 | 90 | 47 | 3 | 109 | 194 | 158 | 245 | 4.74e-12 | 64.7 |
| MsG0380014910.01.T01 | AT4G33920 | 35.398 | 113 | 48 | 2 | 103 | 190 | 49 | 161 | 5.08e-12 | 63.9 |
| MsG0380014910.01.T01 | AT4G33920 | 35.398 | 113 | 48 | 2 | 103 | 190 | 79 | 191 | 5.93e-12 | 63.9 |
| MsG0380014910.01.T01 | AT3G63320 | 41.111 | 90 | 47 | 3 | 109 | 194 | 241 | 328 | 8.40e-12 | 63.9 |
| MsG0380014910.01.T01 | AT4G31860 | 37.624 | 101 | 47 | 3 | 110 | 198 | 191 | 287 | 1.10e-11 | 63.2 |
| MsG0380014910.01.T01 | AT4G31860 | 37.755 | 98 | 45 | 3 | 113 | 198 | 194 | 287 | 1.24e-11 | 63.2 |
| MsG0380014910.01.T01 | AT1G47380 | 28.205 | 195 | 95 | 7 | 42 | 192 | 58 | 251 | 2.35e-11 | 62.8 |
| MsG0380014910.01.T01 | AT2G25070 | 35.714 | 98 | 50 | 2 | 110 | 198 | 191 | 284 | 5.19e-11 | 61.6 |
| MsG0380014910.01.T01 | AT2G25070 | 35.714 | 98 | 50 | 2 | 110 | 198 | 191 | 284 | 5.19e-11 | 61.6 |
| MsG0380014910.01.T01 | AT4G31860 | 38.372 | 86 | 40 | 2 | 113 | 189 | 194 | 275 | 8.69e-11 | 60.5 |
Find 50 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTAGCTTCAGATGGATTA+TGG | 0.192944 | 3:-62339062 | MsG0380014910.01.T01:CDS |
| GATCAACCTATATGTGTATT+TGG | 0.250044 | 3:-62339010 | MsG0380014910.01.T01:CDS |
| TCTTCGTGGTGAAAACAATT+TGG | 0.251614 | 3:-62340133 | MsG0380014910.01.T01:intron |
| GAGAGGGAGGAGGGAGTATA+TGG | 0.257999 | 3:-62340181 | MsG0380014910.01.T01:CDS |
| TTGATTTATGTCGCGGTGTT+TGG | 0.326760 | 3:-62339203 | MsG0380014910.01.T01:CDS |
| AATATTAACAAGTCATGTTC+AGG | 0.369231 | 3:+62339081 | None:intergenic |
| ATTAGCTTCAGATGGATTAT+GGG | 0.376455 | 3:-62339061 | MsG0380014910.01.T01:CDS |
| TCACTTTCCGTCATCCCTTC+CGG | 0.392780 | 3:-62340223 | MsG0380014910.01.T01:CDS |
| CGCGGTGTTTGGAGGATTCA+AGG | 0.409122 | 3:-62339192 | MsG0380014910.01.T01:CDS |
| ATTCTAATAACTTTAGTCTC+AGG | 0.420683 | 3:+62339108 | None:intergenic |
| CAAACGCCAAATACACATAT+AGG | 0.429593 | 3:+62339004 | None:intergenic |
| TGCAAATGCCGCTGAATTTG+CGG | 0.434315 | 3:-62339628 | MsG0380014910.01.T01:CDS |
| TTATTGTAAGAGAGGGAGGA+GGG | 0.452027 | 3:-62340190 | MsG0380014910.01.T01:CDS |
| TAACCTAGAAAAGAACATCT+TGG | 0.457684 | 3:-62339601 | MsG0380014910.01.T01:CDS |
| ATTTATGTCGCGGTGTTTGG+AGG | 0.459287 | 3:-62339200 | MsG0380014910.01.T01:CDS |
| ATGGAAGATCGTTATACAGC+TGG | 0.461700 | 3:-62340162 | MsG0380014910.01.T01:CDS |
| TTTATTGTAAGAGAGGGAGG+AGG | 0.463391 | 3:-62340191 | MsG0380014910.01.T01:CDS |
| ACCTTCAAGGGAAGACGAAA+AGG | 0.493186 | 3:-62339360 | MsG0380014910.01.T01:CDS |
| TTCAGATGGATTATGGGATA+AGG | 0.496992 | 3:-62339055 | MsG0380014910.01.T01:CDS |
| CTTGCTGTATCAAGAGGAAT+CGG | 0.497083 | 3:-62339165 | MsG0380014910.01.T01:CDS |
| TCACCCATTGTTTCAAGTGT+CGG | 0.498005 | 3:+62339139 | None:intergenic |
| CCTCTCTTACAATAAACAAA+AGG | 0.499449 | 3:+62340198 | None:intergenic |
| TTTGGTGTATTTGATGGCCA+TGG | 0.500054 | 3:-62339654 | MsG0380014910.01.T01:CDS |
| ATCTGATCACCGACCTTCAA+GGG | 0.504972 | 3:-62339372 | MsG0380014910.01.T01:CDS |
| CATCTGATCACCGACCTTCA+AGG | 0.507130 | 3:-62339373 | MsG0380014910.01.T01:CDS |
| TTTCGTCTTCCCTTGAAGGT+CGG | 0.508205 | 3:+62339363 | None:intergenic |
| ACAGCTGGGGTTAATCTTCG+TGG | 0.510384 | 3:-62340147 | MsG0380014910.01.T01:CDS |
| TTGTTAATATTAGCTTCAGA+TGG | 0.519141 | 3:-62339069 | MsG0380014910.01.T01:CDS |
| AGACCGACACTTGAAACAAT+GGG | 0.534360 | 3:-62339142 | MsG0380014910.01.T01:CDS |
| TGGAAGATCGTTATACAGCT+GGG | 0.545761 | 3:-62340161 | MsG0380014910.01.T01:CDS |
| CAGCGGCATTTGCACCTCCA+TGG | 0.547238 | 3:+62339637 | None:intergenic |
| TGAACTCAGAATCGGTATTG+AGG | 0.547989 | 3:+62339523 | None:intergenic |
| TTACAATAAACAAAAGGTAC+CGG | 0.558757 | 3:+62340204 | None:intergenic |
| TTGTTTATTGTAAGAGAGGG+AGG | 0.566076 | 3:-62340194 | MsG0380014910.01.T01:CDS |
| GAGACCGACACTTGAAACAA+TGG | 0.575527 | 3:-62339143 | MsG0380014910.01.T01:CDS |
| AAAGGTACCGGAAGGGATGA+CGG | 0.580540 | 3:+62340216 | None:intergenic |
| GGATCTCTTGCTGTATCAAG+AGG | 0.589287 | 3:-62339171 | MsG0380014910.01.T01:CDS |
| AATAAACAAAAGGTACCGGA+AGG | 0.607822 | 3:+62340208 | None:intergenic |
| GAGTGATGAAGATGATGTTG+AGG | 0.608161 | 3:-62339565 | MsG0380014910.01.T01:CDS |
| CTGCTCCCTTACGTTGATCA+CGG | 0.614701 | 3:+62340390 | None:intergenic |
| GTTATTAGCCGCAAATTCAG+CGG | 0.628860 | 3:+62339620 | None:intergenic |
| TAGTAGAGGAGGTGTTGCTG+AGG | 0.637738 | 3:-62339402 | MsG0380014910.01.T01:intron |
| TGATGAAGATGATGTTGAGG+AGG | 0.640938 | 3:-62339562 | MsG0380014910.01.T01:CDS |
| GGTTATGTTGATTTATGTCG+CGG | 0.653499 | 3:-62339210 | MsG0380014910.01.T01:CDS |
| GGTGTATTTGATGGCCATGG+AGG | 0.663870 | 3:-62339651 | MsG0380014910.01.T01:CDS |
| ATAAACAAAAGGTACCGGAA+GGG | 0.664203 | 3:+62340209 | None:intergenic |
| GGAAGATCGTTATACAGCTG+GGG | 0.671830 | 3:-62340160 | MsG0380014910.01.T01:CDS |
| AAAGGACAGAATTGAGACCC+TGG | 0.675506 | 3:-62339342 | MsG0380014910.01.T01:intron |
| AGAAAAGAACATCTTGGACG+AGG | 0.682239 | 3:-62339595 | MsG0380014910.01.T01:CDS |
| GTTGAGGAGGCAGTGAAGCG+CGG | 0.685913 | 3:-62339549 | MsG0380014910.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGAATATATAATATTGATT+TGG | - | Chr3:62339279-62339298 | MsG0380014910.01.T01:intron | 10.0% |
| !!! | TTTCAATATTTGTTTTTTTT+TGG | - | Chr3:62339403-62339422 | MsG0380014910.01.T01:intron | 10.0% |
| !!! | ATATATAATATTGATTTGGA+TGG | - | Chr3:62339283-62339302 | MsG0380014910.01.T01:intron | 15.0% |
| !! | CACAATTCAAATTTAACTAA+TGG | + | Chr3:62339554-62339573 | None:intergenic | 20.0% |
| ! | AAATGGTAAGAAAATTTACC+AGG | + | Chr3:62340067-62340086 | None:intergenic | 25.0% |
| ! | AATATTAACAAGTCATGTTC+AGG | + | Chr3:62340310-62340329 | None:intergenic | 25.0% |
| ! | AATGGTAAGAAAATTTACCA+GGG | + | Chr3:62340066-62340085 | None:intergenic | 25.0% |
| ! | ATTCTAATAACTTTAGTCTC+AGG | + | Chr3:62340283-62340302 | None:intergenic | 25.0% |
| ! | TTTCTTACCATTTGTGTATT+TGG | - | Chr3:62340074-62340093 | MsG0380014910.01.T01:intron | 25.0% |
| !! | GTTGATATGATTTTGTCAAT+GGG | - | Chr3:62339313-62339332 | MsG0380014910.01.T01:intron | 25.0% |
| !! | TGTTGATATGATTTTGTCAA+TGG | - | Chr3:62339312-62339331 | MsG0380014910.01.T01:intron | 25.0% |
| !! | TTACAATAAACAAAAGGTAC+CGG | + | Chr3:62339187-62339206 | None:intergenic | 25.0% |
| !! | TTGTTAATATTAGCTTCAGA+TGG | - | Chr3:62340319-62340338 | MsG0380014910.01.T01:intron | 25.0% |
| !!! | CTTTTGTTTATTGTAAGAGA+GGG | - | Chr3:62339191-62339210 | MsG0380014910.01.T01:CDS | 25.0% |
| !!! | GTTTATTTTGTGATAATTGC+AGG | - | Chr3:62339706-62339725 | MsG0380014910.01.T01:intron | 25.0% |
| !!! | TGTTTTTTTTTGGTCTGATT+TGG | - | Chr3:62339413-62339432 | MsG0380014910.01.T01:intron | 25.0% |
| !!! | TTTTTTCATGAACTCAGAAT+CGG | + | Chr3:62339876-62339895 | None:intergenic | 25.0% |
| AAGAGAACCAAATACACAAA+TGG | + | Chr3:62340084-62340103 | None:intergenic | 30.0% | |
| ACTTGATTTCTTAATTGCTC+TGG | - | Chr3:62339353-62339372 | MsG0380014910.01.T01:CDS | 30.0% | |
| ATGCTTGAAATTTATCTTGC+AGG | - | Chr3:62340153-62340172 | MsG0380014910.01.T01:CDS | 30.0% | |
| CCTCTCTTACAATAAACAAA+AGG | + | Chr3:62339193-62339212 | None:intergenic | 30.0% | |
| GATCAACCTATATGTGTATT+TGG | - | Chr3:62340378-62340397 | MsG0380014910.01.T01:intron | 30.0% | |
| TAACCTAGAAAAGAACATCT+TGG | - | Chr3:62339787-62339806 | MsG0380014910.01.T01:intron | 30.0% | |
| TGCTTGAAATTTATCTTGCA+GGG | - | Chr3:62340154-62340173 | MsG0380014910.01.T01:CDS | 30.0% | |
| TTCATGAAAAAAGATCTGCA+TGG | - | Chr3:62339884-62339903 | MsG0380014910.01.T01:intron | 30.0% | |
| ! | AAAGTCAATTTTAAGCTGCT+AGG | - | Chr3:62339478-62339497 | MsG0380014910.01.T01:intron | 30.0% |
| ! | GTGTAAAATCTAACTTGTAC+AGG | - | Chr3:62339684-62339703 | MsG0380014910.01.T01:intron | 30.0% |
| !! | ATTAGCTTCAGATGGATTAT+GGG | - | Chr3:62340327-62340346 | MsG0380014910.01.T01:intron | 30.0% |
| !! | CCTTTTGTTTATTGTAAGAG+AGG | - | Chr3:62339190-62339209 | MsG0380014910.01.T01:CDS | 30.0% |
| !! | TATTAGCTTCAGATGGATTA+TGG | - | Chr3:62340326-62340345 | MsG0380014910.01.T01:intron | 30.0% |
| !!! | GCTTTTTTTGGTGTATTTGA+TGG | - | Chr3:62339728-62339747 | MsG0380014910.01.T01:intron | 30.0% |
| !!! | TGATAATTGCAGGCTTTTTT+TGG | - | Chr3:62339716-62339735 | MsG0380014910.01.T01:intron | 30.0% |
| AGAACCAAACTCAAAACACA+AGG | + | Chr3:62340126-62340145 | None:intergenic | 35.0% | |
| ATGAAAAAAGATCTGCATGG+TGG | - | Chr3:62339887-62339906 | MsG0380014910.01.T01:intron | 35.0% | |
| CAAACGCCAAATACACATAT+AGG | + | Chr3:62340387-62340406 | None:intergenic | 35.0% | |
| GCTGTGTAACAACATTCATT+AGG | - | Chr3:62339915-62339934 | MsG0380014910.01.T01:intron | 35.0% | |
| GTAACAACATTCATTAGGAG+TGG | - | Chr3:62339920-62339939 | MsG0380014910.01.T01:intron | 35.0% | |
| TCTTCGTGGTGAAAACAATT+TGG | - | Chr3:62339255-62339274 | MsG0380014910.01.T01:intron | 35.0% | |
| TTGAAATTTATCTTGCAGGG+TGG | - | Chr3:62340157-62340176 | MsG0380014910.01.T01:CDS | 35.0% | |
| TTGTTTATTGTAAGAGAGGG+AGG | - | Chr3:62339194-62339213 | MsG0380014910.01.T01:CDS | 35.0% | |
| !! | AATAAACAAAAGGTACCGGA+AGG | + | Chr3:62339183-62339202 | None:intergenic | 35.0% |
| !! | ATAAACAAAAGGTACCGGAA+GGG | + | Chr3:62339182-62339201 | None:intergenic | 35.0% |
| !! | CTTAATTGCTCTGGTCTTAT+TGG | - | Chr3:62339362-62339381 | MsG0380014910.01.T01:CDS | 35.0% |
| !! | GGTTATGTTGATTTATGTCG+CGG | - | Chr3:62340178-62340197 | MsG0380014910.01.T01:CDS | 35.0% |
| !! | TTCAGATGGATTATGGGATA+AGG | - | Chr3:62340333-62340352 | MsG0380014910.01.T01:intron | 35.0% |
| !!! | GAAACCTTGTGTTTTGAGTT+TGG | - | Chr3:62340119-62340138 | MsG0380014910.01.T01:intron | 35.0% |
| ACTTAGTTGTGTCTTAACGC+TGG | - | Chr3:62339945-62339964 | MsG0380014910.01.T01:intron | 40.0% | |
| AGAAAAGAACATCTTGGACG+AGG | - | Chr3:62339793-62339812 | MsG0380014910.01.T01:intron | 40.0% | |
| AGACCGACACTTGAAACAAT+GGG | - | Chr3:62340246-62340265 | MsG0380014910.01.T01:intron | 40.0% | |
| ATGGAAGATCGTTATACAGC+TGG | - | Chr3:62339226-62339245 | MsG0380014910.01.T01:intron | 40.0% | |
| CTCCTCTACTAATAACAGCA+CGG | + | Chr3:62339977-62339996 | None:intergenic | 40.0% | |
| GAGTGATGAAGATGATGTTG+AGG | - | Chr3:62339823-62339842 | MsG0380014910.01.T01:intron | 40.0% | |
| GTTATTAGCCGCAAATTCAG+CGG | + | Chr3:62339771-62339790 | None:intergenic | 40.0% | |
| TGAACTCAGAATCGGTATTG+AGG | + | Chr3:62339868-62339887 | None:intergenic | 40.0% | |
| TGATGAAGATGATGTTGAGG+AGG | - | Chr3:62339826-62339845 | MsG0380014910.01.T01:intron | 40.0% | |
| TGGAAGATCGTTATACAGCT+GGG | - | Chr3:62339227-62339246 | MsG0380014910.01.T01:intron | 40.0% | |
| TTATTGTAAGAGAGGGAGGA+GGG | - | Chr3:62339198-62339217 | MsG0380014910.01.T01:CDS | 40.0% | |
| TTGATTTATGTCGCGGTGTT+TGG | - | Chr3:62340185-62340204 | MsG0380014910.01.T01:CDS | 40.0% | |
| TTTATTGTAAGAGAGGGAGG+AGG | - | Chr3:62339197-62339216 | MsG0380014910.01.T01:CDS | 40.0% | |
| ! | ATATGCTTGCTTGCTTGTTG+TGG | - | Chr3:62339630-62339649 | MsG0380014910.01.T01:CDS | 40.0% |
| ! | CGTCCAAGATGTTCTTTTCT+AGG | + | Chr3:62339793-62339812 | None:intergenic | 40.0% |
| ! | CTTGCTGTATCAAGAGGAAT+CGG | - | Chr3:62340223-62340242 | MsG0380014910.01.T01:CDS | 40.0% |
| ! | TCACCCATTGTTTCAAGTGT+CGG | + | Chr3:62340252-62340271 | None:intergenic | 40.0% |
| ! | TTTGGTGTATTTGATGGCCA+TGG | - | Chr3:62339734-62339753 | MsG0380014910.01.T01:intron | 40.0% |
| !! | TCTGATTTGGTAGCTGCATA+TGG | - | Chr3:62339426-62339445 | MsG0380014910.01.T01:intron | 40.0% |
| AAAGGACAGAATTGAGACCC+TGG | - | Chr3:62340046-62340065 | MsG0380014910.01.T01:intron | 45.0% | |
| ACCTTCAAGGGAAGACGAAA+AGG | - | Chr3:62340028-62340047 | MsG0380014910.01.T01:intron | 45.0% | |
| ATCTGATCACCGACCTTCAA+GGG | - | Chr3:62340016-62340035 | MsG0380014910.01.T01:intron | 45.0% | |
| ATTTATGTCGCGGTGTTTGG+AGG | - | Chr3:62340188-62340207 | MsG0380014910.01.T01:CDS | 45.0% | |
| GAGACCGACACTTGAAACAA+TGG | - | Chr3:62340245-62340264 | MsG0380014910.01.T01:intron | 45.0% | |
| GGAAGATCGTTATACAGCTG+GGG | - | Chr3:62339228-62339247 | MsG0380014910.01.T01:intron | 45.0% | |
| GTCATCCGTGATCAACGTAA+GGG | - | Chr3:62338993-62339012 | MsG0380014910.01.T01:CDS | 45.0% | |
| TGCAAATGCCGCTGAATTTG+CGG | - | Chr3:62339760-62339779 | MsG0380014910.01.T01:intron | 45.0% | |
| TGTCATCCGTGATCAACGTA+AGG | - | Chr3:62338992-62339011 | MsG0380014910.01.T01:CDS | 45.0% | |
| TTTCGTCTTCCCTTGAAGGT+CGG | + | Chr3:62340028-62340047 | None:intergenic | 45.0% | |
| ! | CGTGCTGTTATTAGTAGAGG+AGG | - | Chr3:62339975-62339994 | MsG0380014910.01.T01:intron | 45.0% |
| ! | GGATCTCTTGCTGTATCAAG+AGG | - | Chr3:62340217-62340236 | MsG0380014910.01.T01:CDS | 45.0% |
| ! | TCCTTTTCGTCTTCCCTTGA+AGG | + | Chr3:62340032-62340051 | None:intergenic | 45.0% |
| ! | TGCCGTGCTGTTATTAGTAG+AGG | - | Chr3:62339972-62339991 | MsG0380014910.01.T01:intron | 45.0% |
| ACAGCTGGGGTTAATCTTCG+TGG | - | Chr3:62339241-62339260 | MsG0380014910.01.T01:intron | 50.0% | |
| CATCTGATCACCGACCTTCA+AGG | - | Chr3:62340015-62340034 | MsG0380014910.01.T01:intron | 50.0% | |
| CGGAAAGTGACCTTAGCAAG+AGG | + | Chr3:62339155-62339174 | None:intergenic | 50.0% | |
| CTGCTCCCTTACGTTGATCA+CGG | + | Chr3:62339001-62339020 | None:intergenic | 50.0% | |
| GAACGCAACAGCTACCTCAA+AGG | - | Chr3:62339089-62339108 | MsG0380014910.01.T01:CDS | 50.0% | |
| GTGGGTGTGGGATTGGAAAA+CGG | + | Chr3:62339027-62339046 | None:intergenic | 50.0% | |
| TCACTTTCCGTCATCCCTTC+CGG | - | Chr3:62339165-62339184 | MsG0380014910.01.T01:CDS | 50.0% | |
| ! | GGTGTATTTGATGGCCATGG+AGG | - | Chr3:62339737-62339756 | MsG0380014910.01.T01:intron | 50.0% |
| !! | AAAGGTACCGGAAGGGATGA+CGG | + | Chr3:62339175-62339194 | None:intergenic | 50.0% |
| !! | GCTTGTTGTGGCTGATGACT+TGG | - | Chr3:62339642-62339661 | MsG0380014910.01.T01:CDS | 50.0% |
| !! | TAGTAGAGGAGGTGTTGCTG+AGG | - | Chr3:62339986-62340005 | MsG0380014910.01.T01:intron | 50.0% |
| ATCCCACACCCACCATCGAA+AGG | - | Chr3:62339034-62339053 | MsG0380014910.01.T01:CDS | 55.0% | |
| ATCGACGTCATCCACGATCC+AGG | - | Chr3:62339119-62339138 | MsG0380014910.01.T01:CDS | 55.0% | |
| CGAAGTCGAGGGAACGGAAA+CGG | + | Chr3:62339067-62339086 | None:intergenic | 55.0% | |
| CGCGGTGTTTGGAGGATTCA+AGG | - | Chr3:62340196-62340215 | MsG0380014910.01.T01:CDS | 55.0% | |
| CGTCGATGACGACACCTTTG+AGG | + | Chr3:62339106-62339125 | None:intergenic | 55.0% | |
| CGTGATCAACGTAAGGGAGC+AGG | - | Chr3:62338999-62339018 | MsG0380014910.01.T01:CDS | 55.0% | |
| CGTTTCCGTTCCCTCGACTT+CGG | - | Chr3:62339065-62339084 | MsG0380014910.01.T01:CDS | 55.0% | |
| GAAGTCGAGGGAACGGAAAC+GGG | + | Chr3:62339066-62339085 | None:intergenic | 55.0% | |
| ! | GAGAGGGAGGAGGGAGTATA+TGG | - | Chr3:62339207-62339226 | MsG0380014910.01.T01:CDS | 55.0% |
| !! | GACCTTTCGATGGTGGGTGT+GGG | + | Chr3:62339039-62339058 | None:intergenic | 55.0% |
| !! | TTCGATGGTGGGTGTGGGAT+TGG | + | Chr3:62339034-62339053 | None:intergenic | 55.0% |
| AACGGGCGAGGACCTTTCGA+TGG | + | Chr3:62339049-62339068 | None:intergenic | 60.0% | |
| CAGCGGCATTTGCACCTCCA+TGG | + | Chr3:62339754-62339773 | None:intergenic | 60.0% | |
| GGCGAGGACCTTTCGATGGT+GGG | + | Chr3:62339045-62339064 | None:intergenic | 60.0% | |
| GTCATCCACGATCCAGGTCG+CGG | - | Chr3:62339125-62339144 | MsG0380014910.01.T01:CDS | 60.0% | |
| GTTCGCCGAAGTCGAGGGAA+CGG | + | Chr3:62339073-62339092 | None:intergenic | 60.0% | |
| GTTGAGGAGGCAGTGAAGCG+CGG | - | Chr3:62339839-62339858 | MsG0380014910.01.T01:intron | 60.0% | |
| GTTGCGTTCGCCGAAGTCGA+GGG | + | Chr3:62339078-62339097 | None:intergenic | 60.0% | |
| TCGCGGTGCACCTCTTGCTA+AGG | - | Chr3:62339142-62339161 | MsG0380014910.01.T01:CDS | 60.0% | |
| TGTTGCGTTCGCCGAAGTCG+AGG | + | Chr3:62339079-62339098 | None:intergenic | 60.0% | |
| !! | GGACCTTTCGATGGTGGGTG+TGG | + | Chr3:62339040-62339059 | None:intergenic | 60.0% |
| GGGCGAGGACCTTTCGATGG+TGG | + | Chr3:62339046-62339065 | None:intergenic | 65.0% | |
| CGAGGGAACGGAAACGGGCG+AGG | + | Chr3:62339061-62339080 | None:intergenic | 70.0% | |
| GTGCACCGCGACCTGGATCG+TGG | + | Chr3:62339133-62339152 | None:intergenic | 70.0% | |
| ! | GCAAGAGGTGCACCGCGACC+TGG | + | Chr3:62339140-62339159 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 62338984 | 62340426 | 62338984 | ID=MsG0380014910.01;Name=MsG0380014910.01 |
| Chr3 | mRNA | 62338984 | 62340426 | 62338984 | ID=MsG0380014910.01.T01;Parent=MsG0380014910.01;Name=MsG0380014910.01.T01;_AED=0.47;_eAED=0.49;_QI=0|0|0|0.8|0.75|0.6|5|0|214 |
| Chr3 | exon | 62340402 | 62340426 | 62340402 | ID=MsG0380014910.01.T01:exon:29484;Parent=MsG0380014910.01.T01 |
| Chr3 | exon | 62340134 | 62340246 | 62340134 | ID=MsG0380014910.01.T01:exon:29483;Parent=MsG0380014910.01.T01 |
| Chr3 | exon | 62339502 | 62339682 | 62339502 | ID=MsG0380014910.01.T01:exon:29482;Parent=MsG0380014910.01.T01 |
| Chr3 | exon | 62339343 | 62339416 | 62339343 | ID=MsG0380014910.01.T01:exon:29481;Parent=MsG0380014910.01.T01 |
| Chr3 | exon | 62338984 | 62339235 | 62338984 | ID=MsG0380014910.01.T01:exon:29480;Parent=MsG0380014910.01.T01 |
| Chr3 | CDS | 62340402 | 62340426 | 62340402 | ID=MsG0380014910.01.T01:cds;Parent=MsG0380014910.01.T01 |
| Chr3 | CDS | 62340134 | 62340246 | 62340134 | ID=MsG0380014910.01.T01:cds;Parent=MsG0380014910.01.T01 |
| Chr3 | CDS | 62339502 | 62339682 | 62339502 | ID=MsG0380014910.01.T01:cds;Parent=MsG0380014910.01.T01 |
| Chr3 | CDS | 62339343 | 62339416 | 62339343 | ID=MsG0380014910.01.T01:cds;Parent=MsG0380014910.01.T01 |
| Chr3 | CDS | 62338984 | 62339235 | 62338984 | ID=MsG0380014910.01.T01:cds;Parent=MsG0380014910.01.T01 |
| Gene Sequence |
| Protein sequence |
