Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015174.01.T01 | XP_003600619.2 | 97.073 | 205 | 6 | 0 | 1 | 205 | 1 | 205 | 1.01E-138 | 396 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015174.01.T01 | I3S5Y6 | 97.073 | 205 | 6 | 0 | 1 | 205 | 1 | 205 | 4.84e-139 | 396 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000452.01 | MsG0380015174.01 | 0.857043 | 2.134605e-62 | 5.198638e-59 |
| MsG0380015174.01 | MsG0780041648.01 | 0.870136 | 1.833196e-66 | 7.053355e-63 |
| MsG0280006700.01 | MsG0380015174.01 | 0.856388 | 3.327015e-62 | 7.928337e-59 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015174.01.T01 | MTR_3g064350 | 97.073 | 205 | 6 | 0 | 1 | 205 | 1 | 205 | 1.23e-142 | 396 |
| MsG0380015174.01.T01 | MTR_6g090460 | 47.368 | 114 | 49 | 4 | 76 | 187 | 64 | 168 | 1.22e-21 | 87.4 |
| MsG0380015174.01.T01 | MTR_8g102350 | 55.556 | 63 | 26 | 2 | 75 | 135 | 75 | 137 | 9.12e-15 | 69.3 |
| MsG0380015174.01.T01 | MTR_3g105210 | 42.045 | 88 | 47 | 2 | 74 | 157 | 54 | 141 | 3.46e-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015174.01.T01 | AT5G56550 | 51.613 | 93 | 42 | 1 | 75 | 164 | 74 | 166 | 5.54e-18 | 78.2 |
| MsG0380015174.01.T01 | AT4G26288 | 56.061 | 66 | 21 | 2 | 75 | 136 | 59 | 120 | 8.38e-13 | 63.9 |
| MsG0380015174.01.T01 | AT5G21940 | 50.847 | 59 | 21 | 1 | 75 | 125 | 74 | 132 | 7.82e-11 | 60.1 |
| MsG0380015174.01.T01 | AT3G43850 | 44.444 | 81 | 38 | 2 | 75 | 148 | 51 | 131 | 8.57e-11 | 59.3 |
Find 49 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAAGTGGAAGTAACTTTA+TGG | 0.218762 | 3:+66235222 | MsG0380015174.01.T01:CDS |
| TAAAATGAGGTGATATAGTT+TGG | 0.239625 | 3:-66234473 | None:intergenic |
| TAGATTTAAAACTTGATTCT+TGG | 0.251748 | 3:-66235123 | None:intergenic |
| TTCTTTGGTGATTCAATCAT+AGG | 0.303697 | 3:-66234598 | None:intergenic |
| GAGAAAGAAACTTACTTGAT+TGG | 0.349701 | 3:-66234835 | None:intergenic |
| AAAATGAGGTGATATAGTTT+GGG | 0.350977 | 3:-66234472 | None:intergenic |
| AACTGCTTTGTTTGCTTGAA+AGG | 0.362454 | 3:+66235309 | MsG0380015174.01.T01:exon |
| AACAAATGTGAAGAGTTTAG+AGG | 0.373645 | 3:+66235027 | MsG0380015174.01.T01:CDS |
| AACTATGATGATATTATAGA+TGG | 0.388503 | 3:+66234637 | MsG0380015174.01.T01:CDS |
| CTCTTGTTGTAAGGACTCTC+TGG | 0.389294 | 3:-66235061 | None:intergenic |
| TGCGGATATCTTGTAGTGTA+AGG | 0.400519 | 3:+66235395 | MsG0380015174.01.T01:three_prime_UTR |
| TTCTTCTTTGAATGCAAGAA+AGG | 0.409881 | 3:+66235201 | MsG0380015174.01.T01:CDS |
| GACTTCAACCTCTTGTTGTA+AGG | 0.410223 | 3:-66235070 | None:intergenic |
| TAAAAGATTGTGACTTTCCT+TGG | 0.414868 | 3:-66234997 | None:intergenic |
| GATCTATTATGAGGATGAAT+TGG | 0.430717 | 3:-66235256 | None:intergenic |
| TCTGATTCTGAAGTTTCAAA+TGG | 0.436088 | 3:+66234673 | MsG0380015174.01.T01:CDS |
| ACTGCTTTGTTTGCTTGAAA+GGG | 0.445401 | 3:+66235310 | MsG0380015174.01.T01:exon |
| GGAAGTGGAAGTAACTTTAT+GGG | 0.451892 | 3:+66235223 | MsG0380015174.01.T01:CDS |
| GGGTGAATTCAGCAAGTTCA+AGG | 0.456612 | 3:+66235170 | MsG0380015174.01.T01:CDS |
| GAAGTAACTTTATGGGTAGT+AGG | 0.457592 | 3:+66235230 | MsG0380015174.01.T01:CDS |
| TTGAAGTCATGTAGAAGCTA+TGG | 0.472853 | 3:+66235085 | MsG0380015174.01.T01:CDS |
| TGTCAAGGCTTGTTTCTAAG+AGG | 0.473310 | 3:+66235146 | MsG0380015174.01.T01:CDS |
| ATTTATGGAGCTGTGATTTG+CGG | 0.484670 | 3:+66235377 | MsG0380015174.01.T01:three_prime_UTR |
| GTGTTTGTAATTGTTGCTTG+TGG | 0.494459 | 3:+66234521 | MsG0380015174.01.T01:five_prime_UTR |
| ATGAAAGAAAAGTGTTGGAG+AGG | 0.494857 | 3:+66235460 | MsG0380015174.01.T01:three_prime_UTR |
| GAGGATGAAGAAGATGAAGT+AGG | 0.495656 | 3:-66234748 | None:intergenic |
| AAAGAAGACATATCATTCAA+TGG | 0.508412 | 3:-66234799 | None:intergenic |
| GTCAAGGCTTGTTTCTAAGA+GGG | 0.514801 | 3:+66235147 | MsG0380015174.01.T01:CDS |
| AGTTTGATTAGAGATATTGT+TGG | 0.516221 | 3:-66235290 | None:intergenic |
| CTAGTAGATGATCTATTATG+AGG | 0.516955 | 3:-66235265 | None:intergenic |
| TTGAATGCAAGAAAGGGAAG+TGG | 0.520993 | 3:+66235208 | MsG0380015174.01.T01:CDS |
| TCTTCTTTGAATGCAAGAAA+GGG | 0.526225 | 3:+66235202 | MsG0380015174.01.T01:CDS |
| TGAAAGAAAAGTGTTGGAGA+GGG | 0.526991 | 3:+66235461 | MsG0380015174.01.T01:three_prime_UTR |
| ACATGCACTTTAATTTGTCT+AGG | 0.527593 | 3:-66234500 | None:intergenic |
| CAGAGAGTCCTTACAACAAG+AGG | 0.555710 | 3:+66235062 | MsG0380015174.01.T01:CDS |
| TGAAGATATCTAATTAGAGA+TGG | 0.556375 | 3:+66234558 | MsG0380015174.01.T01:exon |
| CAAGGCTTGTTTCTAAGAGG+GGG | 0.561375 | 3:+66235149 | MsG0380015174.01.T01:CDS |
| GGTGAATTCAGCAAGTTCAA+GGG | 0.566478 | 3:+66235171 | MsG0380015174.01.T01:CDS |
| AAGTCATGTAGAAGCTATGG+AGG | 0.581269 | 3:+66235088 | MsG0380015174.01.T01:CDS |
| CTATTATGAGGATGAATTGG+TGG | 0.590564 | 3:-66235253 | None:intergenic |
| GTGAATTCAGCAAGTTCAAG+GGG | 0.594667 | 3:+66235172 | MsG0380015174.01.T01:CDS |
| GAAAGAAAAGTGTTGGAGAG+GGG | 0.597518 | 3:+66235462 | MsG0380015174.01.T01:three_prime_UTR |
| TCAAGGCTTGTTTCTAAGAG+GGG | 0.607183 | 3:+66235148 | MsG0380015174.01.T01:CDS |
| TTGTCTAGGAAAATAAAATG+AGG | 0.609967 | 3:-66234486 | None:intergenic |
| GGACTTTCAAAATTCTACCA+AGG | 0.610945 | 3:+66234980 | MsG0380015174.01.T01:CDS |
| AAATGAGGTGATATAGTTTG+GGG | 0.632419 | 3:-66234471 | None:intergenic |
| AAGGCTTGTTTCTAAGAGGG+GGG | 0.642551 | 3:+66235150 | MsG0380015174.01.T01:CDS |
| AGTCATGTAGAAGCTATGGA+GGG | 0.680331 | 3:+66235089 | MsG0380015174.01.T01:CDS |
| TTCAAGTTGATGAGTAGATG+AGG | 0.689907 | 3:-66234767 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAACTATAAAAATTGA+TGG | - | Chr3:66234860-66234879 | None:intergenic | 10.0% |
| !!! | TTTTTTTTGCTTTTTTTAAT+GGG | + | Chr3:66234873-66234892 | MsG0380015174.01.T01:intron | 10.0% |
| !!! | AAAGTTTGTGTTTTTTTTAT+AGG | + | Chr3:66234954-66234973 | MsG0380015174.01.T01:intron | 15.0% |
| !!! | GTTTTTTTTGCTTTTTTTAA+TGG | + | Chr3:66234872-66234891 | MsG0380015174.01.T01:intron | 15.0% |
| !!! | TTTTTGATGTTGTTAATTTA+TGG | + | Chr3:66235362-66235381 | MsG0380015174.01.T01:three_prime_UTR | 15.0% |
| !! | AACTATGATGATATTATAGA+TGG | + | Chr3:66234637-66234656 | MsG0380015174.01.T01:CDS | 20.0% |
| !!! | TAGATTTAAAACTTGATTCT+TGG | - | Chr3:66235126-66235145 | None:intergenic | 20.0% |
| !!! | TTATTTCCATATTTGCTTTT+GGG | + | Chr3:66234905-66234924 | MsG0380015174.01.T01:intron | 20.0% |
| !!! | TTTATTTCCATATTTGCTTT+TGG | + | Chr3:66234904-66234923 | MsG0380015174.01.T01:intron | 20.0% |
| !!! | TTTGTGTTTTTTTTATAGGA+GGG | + | Chr3:66234958-66234977 | MsG0380015174.01.T01:intron | 20.0% |
| ! | AAAGAAGACATATCATTCAA+TGG | - | Chr3:66234802-66234821 | None:intergenic | 25.0% |
| ! | AGTTTGATTAGAGATATTGT+TGG | - | Chr3:66235293-66235312 | None:intergenic | 25.0% |
| ! | TGAAGATATCTAATTAGAGA+TGG | + | Chr3:66234558-66234577 | MsG0380015174.01.T01:exon | 25.0% |
| ! | TTGTCTAGGAAAATAAAATG+AGG | - | Chr3:66234489-66234508 | None:intergenic | 25.0% |
| !! | TCATCATAGTTTTCATGAAA+TGG | - | Chr3:66234628-66234647 | None:intergenic | 25.0% |
| !!! | CAAGTTTTAAATCTATGTCA+AGG | + | Chr3:66235131-66235150 | MsG0380015174.01.T01:CDS | 25.0% |
| !!! | GTTTGTGTTTTTTTTATAGG+AGG | + | Chr3:66234957-66234976 | MsG0380015174.01.T01:intron | 25.0% |
| !!! | TTGTGTTTTTTTTATAGGAG+GGG | + | Chr3:66234959-66234978 | MsG0380015174.01.T01:intron | 25.0% |
| !!! | TTTTGATGAAAGAAAAGTGT+TGG | + | Chr3:66235455-66235474 | MsG0380015174.01.T01:three_prime_UTR | 25.0% |
| AACAAATGTGAAGAGTTTAG+AGG | + | Chr3:66235027-66235046 | MsG0380015174.01.T01:CDS | 30.0% | |
| CTAGTAGATGATCTATTATG+AGG | - | Chr3:66235268-66235287 | None:intergenic | 30.0% | |
| GAGAAAGAAACTTACTTGAT+TGG | - | Chr3:66234838-66234857 | None:intergenic | 30.0% | |
| GATCTATTATGAGGATGAAT+TGG | - | Chr3:66235259-66235278 | None:intergenic | 30.0% | |
| TAAAAGATTGTGACTTTCCT+TGG | - | Chr3:66235000-66235019 | None:intergenic | 30.0% | |
| TCTTCTTTGAATGCAAGAAA+GGG | + | Chr3:66235202-66235221 | MsG0380015174.01.T01:CDS | 30.0% | |
| TTCTTCTTTGAATGCAAGAA+AGG | + | Chr3:66235201-66235220 | MsG0380015174.01.T01:CDS | 30.0% | |
| ! | TCATGAAATGGTCTTTTCTT+TGG | - | Chr3:66234616-66234635 | None:intergenic | 30.0% |
| ! | TTCTTTGGTGATTCAATCAT+AGG | - | Chr3:66234601-66234620 | None:intergenic | 30.0% |
| !! | ACATGCACTTTAATTTGTCT+AGG | - | Chr3:66234503-66234522 | None:intergenic | 30.0% |
| !! | TCTGATTCTGAAGTTTCAAA+TGG | + | Chr3:66234673-66234692 | MsG0380015174.01.T01:CDS | 30.0% |
| ATTTATGGAGCTGTGATTTG+CGG | + | Chr3:66235377-66235396 | MsG0380015174.01.T01:three_prime_UTR | 35.0% | |
| CAATAGCCCAAAAGCAAATA+TGG | - | Chr3:66234914-66234933 | None:intergenic | 35.0% | |
| CTATTATGAGGATGAATTGG+TGG | - | Chr3:66235256-66235275 | None:intergenic | 35.0% | |
| GAAGTAACTTTATGGGTAGT+AGG | + | Chr3:66235230-66235249 | MsG0380015174.01.T01:CDS | 35.0% | |
| GGAAGTGGAAGTAACTTTAT+GGG | + | Chr3:66235223-66235242 | MsG0380015174.01.T01:CDS | 35.0% | |
| GGACTTTCAAAATTCTACCA+AGG | + | Chr3:66234980-66234999 | MsG0380015174.01.T01:CDS | 35.0% | |
| TTGAAGTCATGTAGAAGCTA+TGG | + | Chr3:66235085-66235104 | MsG0380015174.01.T01:CDS | 35.0% | |
| ! | GTGTTTGTAATTGTTGCTTG+TGG | + | Chr3:66234521-66234540 | MsG0380015174.01.T01:five_prime_UTR | 35.0% |
| !! | AACTGCTTTGTTTGCTTGAA+AGG | + | Chr3:66235309-66235328 | MsG0380015174.01.T01:exon | 35.0% |
| !! | ACTGCTTTGTTTGCTTGAAA+GGG | + | Chr3:66235310-66235329 | MsG0380015174.01.T01:exon | 35.0% |
| !! | ATGAAAGAAAAGTGTTGGAG+AGG | + | Chr3:66235460-66235479 | MsG0380015174.01.T01:three_prime_UTR | 35.0% |
| !! | TGAAAGAAAAGTGTTGGAGA+GGG | + | Chr3:66235461-66235480 | MsG0380015174.01.T01:three_prime_UTR | 35.0% |
| !! | TTCAAGTTGATGAGTAGATG+AGG | - | Chr3:66234770-66234789 | None:intergenic | 35.0% |
| AAGTCATGTAGAAGCTATGG+AGG | + | Chr3:66235088-66235107 | MsG0380015174.01.T01:CDS | 40.0% | |
| AGTCATGTAGAAGCTATGGA+GGG | + | Chr3:66235089-66235108 | MsG0380015174.01.T01:CDS | 40.0% | |
| GAGGATGAAGAAGATGAAGT+AGG | - | Chr3:66234751-66234770 | None:intergenic | 40.0% | |
| GGGAAGTGGAAGTAACTTTA+TGG | + | Chr3:66235222-66235241 | MsG0380015174.01.T01:CDS | 40.0% | |
| GGTGAATTCAGCAAGTTCAA+GGG | + | Chr3:66235171-66235190 | MsG0380015174.01.T01:CDS | 40.0% | |
| GTGAATTCAGCAAGTTCAAG+GGG | + | Chr3:66235172-66235191 | MsG0380015174.01.T01:CDS | 40.0% | |
| TGCGGATATCTTGTAGTGTA+AGG | + | Chr3:66235395-66235414 | MsG0380015174.01.T01:three_prime_UTR | 40.0% | |
| TTGAATGCAAGAAAGGGAAG+TGG | + | Chr3:66235208-66235227 | MsG0380015174.01.T01:CDS | 40.0% | |
| ! | GACTTCAACCTCTTGTTGTA+AGG | - | Chr3:66235073-66235092 | None:intergenic | 40.0% |
| !! | GAAAGAAAAGTGTTGGAGAG+GGG | + | Chr3:66235462-66235481 | MsG0380015174.01.T01:three_prime_UTR | 40.0% |
| !! | GTCAAGGCTTGTTTCTAAGA+GGG | + | Chr3:66235147-66235166 | MsG0380015174.01.T01:CDS | 40.0% |
| !! | TCAAGGCTTGTTTCTAAGAG+GGG | + | Chr3:66235148-66235167 | MsG0380015174.01.T01:CDS | 40.0% |
| !! | TGTCAAGGCTTGTTTCTAAG+AGG | + | Chr3:66235146-66235165 | MsG0380015174.01.T01:CDS | 40.0% |
| CAGAGAGTCCTTACAACAAG+AGG | + | Chr3:66235062-66235081 | MsG0380015174.01.T01:CDS | 45.0% | |
| GGGTGAATTCAGCAAGTTCA+AGG | + | Chr3:66235170-66235189 | MsG0380015174.01.T01:CDS | 45.0% | |
| ! | CTCTTGTTGTAAGGACTCTC+TGG | - | Chr3:66235064-66235083 | None:intergenic | 45.0% |
| !! | AAGGCTTGTTTCTAAGAGGG+GGG | + | Chr3:66235150-66235169 | MsG0380015174.01.T01:CDS | 45.0% |
| !! | CAAGGCTTGTTTCTAAGAGG+GGG | + | Chr3:66235149-66235168 | MsG0380015174.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 66234474 | 66235517 | 66234474 | ID=MsG0380015174.01;Name=MsG0380015174.01 |
| Chr3 | mRNA | 66234474 | 66235517 | 66234474 | ID=MsG0380015174.01.T01;Parent=MsG0380015174.01;Name=MsG0380015174.01.T01;_AED=0.24;_eAED=0.24;_QI=103|1|1|1|1|1|2|190|205 |
| Chr3 | exon | 66234474 | 66234842 | 66234474 | ID=MsG0380015174.01.T01:exon:22945;Parent=MsG0380015174.01.T01 |
| Chr3 | exon | 66234976 | 66235517 | 66234976 | ID=MsG0380015174.01.T01:exon:22946;Parent=MsG0380015174.01.T01 |
| Chr3 | five_prime_UTR | 66234474 | 66234576 | 66234474 | ID=MsG0380015174.01.T01:five_prime_utr;Parent=MsG0380015174.01.T01 |
| Chr3 | CDS | 66234577 | 66234842 | 66234577 | ID=MsG0380015174.01.T01:cds;Parent=MsG0380015174.01.T01 |
| Chr3 | CDS | 66234976 | 66235327 | 66234976 | ID=MsG0380015174.01.T01:cds;Parent=MsG0380015174.01.T01 |
| Chr3 | three_prime_UTR | 66235328 | 66235517 | 66235328 | ID=MsG0380015174.01.T01:three_prime_utr;Parent=MsG0380015174.01.T01 |
| Gene Sequence |
| Protein sequence |
