Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015191.01.T01 | XP_018459315.1 | 45.773 | 343 | 162 | 8 | 1 | 342 | 1 | 320 | 3.24E-94 | 293 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015191.01.T01 | A0A6J0LGH1 | 45.773 | 343 | 162 | 8 | 1 | 342 | 1 | 320 | 1.55e-94 | 293 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012192.01 | MsG0380015191.01 | 0.820644 | 6.152571e-53 | 4.895668e-50 |
| MsG0380015191.01 | MsG0580025014.01 | 0.865911 | 4.189158e-65 | 1.386017e-61 |
| MsG0280007556.01 | MsG0380015191.01 | 0.803015 | 4.265616e-49 | 2.120264e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015191.01.T01 | MTR_7g062720 | 39.865 | 148 | 81 | 3 | 1 | 146 | 1 | 142 | 2.88e-31 | 116 |
| MsG0380015191.01.T01 | MTR_4g037355 | 39.007 | 141 | 56 | 4 | 155 | 290 | 1 | 116 | 1.73e-21 | 89.4 |
| MsG0380015191.01.T01 | MTR_4g112730 | 57.971 | 69 | 28 | 1 | 143 | 211 | 2 | 69 | 1.09e-17 | 77.8 |
| MsG0380015191.01.T01 | MTR_5g061150 | 32.432 | 111 | 75 | 0 | 40 | 150 | 26 | 136 | 3.41e-17 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 61 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATCAATGGAACAAATTA+AGG | 0.196151 | 3:+66499114 | MsG0380015191.01.T01:CDS |
| AGTCTTATTGCCCTCATTAA+TGG | 0.201947 | 3:+66499094 | MsG0380015191.01.T01:CDS |
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 3:-66499410 | None:intergenic |
| CACGACCTCTACCACGAAAA+TGG | 0.218437 | 3:-66499526 | None:intergenic |
| CAAATGATCCCAAGATCTTA+TGG | 0.231862 | 3:+66499036 | MsG0380015191.01.T01:CDS |
| GACAAAATTAAAGCAAGATT+TGG | 0.252197 | 3:+66499059 | MsG0380015191.01.T01:CDS |
| GATTCTTGAGGGAGACGATT+TGG | 0.271296 | 3:+66498917 | MsG0380015191.01.T01:CDS |
| CTCAATTAAGTTGTTGTTCC+AGG | 0.274617 | 3:-66498868 | None:intergenic |
| ATTTACTGAATACTCTGATT+TGG | 0.325598 | 3:+66499304 | MsG0380015191.01.T01:CDS |
| AGACAGGTAGTGTCATTCTT+TGG | 0.342502 | 3:-66499782 | None:intergenic |
| CCTGATGATTCTTGTTTGAT+TGG | 0.348440 | 3:-66499586 | None:intergenic |
| ATTCTTGAGGGAGACGATTT+GGG | 0.354692 | 3:+66498918 | MsG0380015191.01.T01:CDS |
| TATTTCTAGAGGGACCTTCC+TGG | 0.364970 | 3:-66499625 | None:intergenic |
| CATATCCACCACGTCCTCTT+TGG | 0.374344 | 3:-66499460 | None:intergenic |
| ACAAAATTAAAGCAAGATTT+GGG | 0.407053 | 3:+66499060 | MsG0380015191.01.T01:CDS |
| CGTGGTGGATATGCTCGTTT+TGG | 0.409902 | 3:+66499470 | MsG0380015191.01.T01:CDS |
| CAAAGAATCTGTGGATGAAA+AGG | 0.424603 | 3:+66499730 | MsG0380015191.01.T01:CDS |
| CCAATCAAACAAGAATCATC+AGG | 0.430863 | 3:+66499586 | MsG0380015191.01.T01:CDS |
| TGCCCTCATTAATGGATCAA+TGG | 0.432677 | 3:+66499102 | MsG0380015191.01.T01:CDS |
| TATAACTGAAGTGGAAAAGT+TGG | 0.441180 | 3:+66499217 | MsG0380015191.01.T01:CDS |
| ACGACCTCTACCACGAAAAT+GGG | 0.460127 | 3:-66499525 | None:intergenic |
| AAATGATCCCAAGATCTTAT+GGG | 0.461716 | 3:+66499037 | MsG0380015191.01.T01:CDS |
| CGTGGTAGAGGTCGTGGAAG+AGG | 0.462783 | 3:+66499533 | MsG0380015191.01.T01:CDS |
| ATCTGTGGATGAAAAGGAAA+AGG | 0.463214 | 3:+66499736 | MsG0380015191.01.T01:CDS |
| AACCACCAGTTGCGTCCTAC+AGG | 0.478404 | 3:+66499374 | MsG0380015191.01.T01:CDS |
| TTGTTCCTGTAGGACGCAAC+TGG | 0.480977 | 3:-66499379 | None:intergenic |
| ATCAAGCACCACATTGATGA+TGG | 0.492315 | 3:+66498990 | MsG0380015191.01.T01:CDS |
| AACAAGAATCATCAGGGTAA+AGG | 0.500113 | 3:+66499593 | MsG0380015191.01.T01:CDS |
| CTGGCGACAACTACATAACC+TGG | 0.502097 | 3:+66498850 | MsG0380015191.01.T01:CDS |
| GATGCGGAAAGAAAGGTCAT+TGG | 0.503149 | 3:+66499666 | MsG0380015191.01.T01:CDS |
| ATGCGGAAAGAAAGGTCATT+GGG | 0.509313 | 3:+66499667 | MsG0380015191.01.T01:CDS |
| GTTCCATTGATCCATTAATG+AGG | 0.509644 | 3:-66499105 | None:intergenic |
| TGTTATAGATGCGGAAAGAA+AGG | 0.513070 | 3:+66499659 | MsG0380015191.01.T01:CDS |
| GGACTAGACAAGATTCTTGA+GGG | 0.521095 | 3:+66498906 | MsG0380015191.01.T01:CDS |
| TGCAACAACAATATAGAATG+AGG | 0.523271 | 3:+66499279 | MsG0380015191.01.T01:CDS |
| TTTGATTGGTCATATTTGAG+AGG | 0.528343 | 3:-66499572 | None:intergenic |
| TTCCATTGATCCATTAATGA+GGG | 0.533283 | 3:-66499104 | None:intergenic |
| TGGCAAAACTATAACTGAAG+TGG | 0.552382 | 3:+66499208 | MsG0380015191.01.T01:CDS |
| AAAATCAGCAGCCTCAAGAC+AGG | 0.562896 | 3:-66499798 | None:intergenic |
| GTCATTGGGCTAAAACATGC+AGG | 0.564978 | 3:+66499681 | MsG0380015191.01.T01:CDS |
| AATTGAGTATCTTGCATGTG+AGG | 0.565013 | 3:+66498884 | MsG0380015191.01.T01:CDS |
| CCACGAAAATGGGTTCTGCT+AGG | 0.571397 | 3:-66499515 | None:intergenic |
| ATTGAGTATCTTGCATGTGA+GGG | 0.575966 | 3:+66498885 | MsG0380015191.01.T01:CDS |
| GGATATGCCATTGTTCCTGT+AGG | 0.578642 | 3:-66499389 | None:intergenic |
| TTTGGTTGCAGCTCTTCTAG+TGG | 0.579996 | 3:+66499322 | MsG0380015191.01.T01:CDS |
| ACTATCATCATTATTTCTAG+AGG | 0.581988 | 3:-66499636 | None:intergenic |
| TTCCTGTAGGACGCAACTGG+TGG | 0.593030 | 3:-66499376 | None:intergenic |
| CTATCATCATTATTTCTAGA+GGG | 0.603915 | 3:-66499635 | None:intergenic |
| GCAACAACAATATAGAATGA+GGG | 0.604762 | 3:+66499280 | MsG0380015191.01.T01:CDS |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 3:-66499423 | None:intergenic |
| TATAATCGCCAAAGAGGACG+TGG | 0.608957 | 3:+66499452 | MsG0380015191.01.T01:CDS |
| GGGACTAGACAAGATTCTTG+AGG | 0.613207 | 3:+66498905 | MsG0380015191.01.T01:CDS |
| GATGATAGTTGTTATAGATG+CGG | 0.615209 | 3:+66499650 | MsG0380015191.01.T01:CDS |
| TAAAAGATACAAAGAATCTG+TGG | 0.628320 | 3:+66499721 | MsG0380015191.01.T01:CDS |
| CAATCAAACAAGAATCATCA+GGG | 0.632179 | 3:+66499587 | MsG0380015191.01.T01:CDS |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 3:+66499446 | MsG0380015191.01.T01:CDS |
| GAATGACACTACCTGTCTTG+AGG | 0.649563 | 3:+66499787 | MsG0380015191.01.T01:CDS |
| GTTGCGTCCTACAGGAACAA+TGG | 0.661047 | 3:+66499382 | MsG0380015191.01.T01:CDS |
| AATCGCCAAAGAGGACGTGG+TGG | 0.675947 | 3:+66499455 | MsG0380015191.01.T01:CDS |
| TTTGTAATCCATCATCAATG+TGG | 0.715751 | 3:-66498998 | None:intergenic |
| CGATTTGGGAGATGTCCCAG+AGG | 0.725435 | 3:+66498932 | MsG0380015191.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATTAAAGCAAGATTT+GGG | + | Chr3:66499060-66499079 | MsG0380015191.01.T01:CDS | 20.0% |
| !!! | TTCAAAATTTTAAGCATAAC+TGG | + | Chr3:66498831-66498850 | MsG0380015191.01.T01:CDS | 20.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | + | Chr3:66499304-66499323 | MsG0380015191.01.T01:CDS | 25.0% |
| ! | GACAAAATTAAAGCAAGATT+TGG | + | Chr3:66499059-66499078 | MsG0380015191.01.T01:CDS | 25.0% |
| ! | TAAAAGATACAAAGAATCTG+TGG | + | Chr3:66499721-66499740 | MsG0380015191.01.T01:CDS | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | + | Chr3:66499188-66499207 | MsG0380015191.01.T01:CDS | 25.0% |
| !!! | ACTATCATCATTATTTCTAG+AGG | - | Chr3:66499639-66499658 | None:intergenic | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | - | Chr3:66499142-66499161 | None:intergenic | 25.0% |
| !!! | CTATCATCATTATTTCTAGA+GGG | - | Chr3:66499638-66499657 | None:intergenic | 25.0% |
| !!! | TGTTGAAGGAGATTTTAATA+TGG | + | Chr3:66499820-66499839 | MsG0380015191.01.T01:CDS | 25.0% |
| !!! | TTTTCTTTGATAATTCCTCT+GGG | - | Chr3:66498950-66498969 | None:intergenic | 25.0% |
| !!! | TTTTTCTTTGATAATTCCTC+TGG | - | Chr3:66498951-66498970 | None:intergenic | 25.0% |
| AAATGATCCCAAGATCTTAT+GGG | + | Chr3:66499037-66499056 | MsG0380015191.01.T01:CDS | 30.0% | |
| CAATCAAACAAGAATCATCA+GGG | + | Chr3:66499587-66499606 | MsG0380015191.01.T01:CDS | 30.0% | |
| GATGATAGTTGTTATAGATG+CGG | + | Chr3:66499650-66499669 | MsG0380015191.01.T01:CDS | 30.0% | |
| GCAACAACAATATAGAATGA+GGG | + | Chr3:66499280-66499299 | MsG0380015191.01.T01:CDS | 30.0% | |
| TATAACTGAAGTGGAAAAGT+TGG | + | Chr3:66499217-66499236 | MsG0380015191.01.T01:CDS | 30.0% | |
| TGCAACAACAATATAGAATG+AGG | + | Chr3:66499279-66499298 | MsG0380015191.01.T01:CDS | 30.0% | |
| TGGATCAATGGAACAAATTA+AGG | + | Chr3:66499114-66499133 | MsG0380015191.01.T01:CDS | 30.0% | |
| TTCCATTGATCCATTAATGA+GGG | - | Chr3:66499107-66499126 | None:intergenic | 30.0% | |
| TTTGTAATCCATCATCAATG+TGG | - | Chr3:66499001-66499020 | None:intergenic | 30.0% | |
| ! | AATTTTGTCCCATAAGATCT+TGG | - | Chr3:66499048-66499067 | None:intergenic | 30.0% |
| ! | ATTTTGTCCCATAAGATCTT+GGG | - | Chr3:66499047-66499066 | None:intergenic | 30.0% |
| ! | TATCTTTTACACAGATACTC+TGG | - | Chr3:66499710-66499729 | None:intergenic | 30.0% |
| ! | TTTGATTGGTCATATTTGAG+AGG | - | Chr3:66499575-66499594 | None:intergenic | 30.0% |
| AAAGGTAAAAACATCCAGGA+AGG | + | Chr3:66499611-66499630 | MsG0380015191.01.T01:CDS | 35.0% | |
| AAAGTTGTGGCGTTTATTTC+AGG | - | Chr3:66499413-66499432 | None:intergenic | 35.0% | |
| AACAAGAATCATCAGGGTAA+AGG | + | Chr3:66499593-66499612 | MsG0380015191.01.T01:CDS | 35.0% | |
| AATTGAGTATCTTGCATGTG+AGG | + | Chr3:66498884-66498903 | MsG0380015191.01.T01:CDS | 35.0% | |
| ATCTGTGGATGAAAAGGAAA+AGG | + | Chr3:66499736-66499755 | MsG0380015191.01.T01:CDS | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | + | Chr3:66498885-66498904 | MsG0380015191.01.T01:CDS | 35.0% | |
| CAAAGAATCTGTGGATGAAA+AGG | + | Chr3:66499730-66499749 | MsG0380015191.01.T01:CDS | 35.0% | |
| CAAATGATCCCAAGATCTTA+TGG | + | Chr3:66499036-66499055 | MsG0380015191.01.T01:CDS | 35.0% | |
| CCAATCAAACAAGAATCATC+AGG | + | Chr3:66499586-66499605 | MsG0380015191.01.T01:CDS | 35.0% | |
| GTTCCATTGATCCATTAATG+AGG | - | Chr3:66499108-66499127 | None:intergenic | 35.0% | |
| TGGCAAAACTATAACTGAAG+TGG | + | Chr3:66499208-66499227 | MsG0380015191.01.T01:CDS | 35.0% | |
| TGTTATAGATGCGGAAAGAA+AGG | + | Chr3:66499659-66499678 | MsG0380015191.01.T01:CDS | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | + | Chr3:66499419-66499438 | MsG0380015191.01.T01:CDS | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | + | Chr3:66499420-66499439 | MsG0380015191.01.T01:CDS | 35.0% |
| ! | CCTGATGATTCTTGTTTGAT+TGG | - | Chr3:66499589-66499608 | None:intergenic | 35.0% |
| ! | CTCAATTAAGTTGTTGTTCC+AGG | - | Chr3:66498871-66498890 | None:intergenic | 35.0% |
| !! | AGTCTTATTGCCCTCATTAA+TGG | + | Chr3:66499094-66499113 | MsG0380015191.01.T01:CDS | 35.0% |
| ACGCCCTTGATTAAAAGTTG+TGG | - | Chr3:66499426-66499445 | None:intergenic | 40.0% | |
| ATCAAGCACCACATTGATGA+TGG | + | Chr3:66498990-66499009 | MsG0380015191.01.T01:CDS | 40.0% | |
| ATGCGGAAAGAAAGGTCATT+GGG | + | Chr3:66499667-66499686 | MsG0380015191.01.T01:CDS | 40.0% | |
| ATTCTTGAGGGAGACGATTT+GGG | + | Chr3:66498918-66498937 | MsG0380015191.01.T01:CDS | 40.0% | |
| GGACTAGACAAGATTCTTGA+GGG | + | Chr3:66498906-66498925 | MsG0380015191.01.T01:CDS | 40.0% | |
| GGGTAAAGGTAAAAACATCC+AGG | + | Chr3:66499607-66499626 | MsG0380015191.01.T01:CDS | 40.0% | |
| ! | AACTTTTCATGCATCTCAGG+TGG | + | Chr3:66499253-66499272 | MsG0380015191.01.T01:CDS | 40.0% |
| ! | ACAACTTTTAATCAAGGGCG+TGG | + | Chr3:66499425-66499444 | MsG0380015191.01.T01:CDS | 40.0% |
| ! | AGACAGGTAGTGTCATTCTT+TGG | - | Chr3:66499785-66499804 | None:intergenic | 40.0% |
| ! | CTCAACTTTTCATGCATCTC+AGG | + | Chr3:66499250-66499269 | MsG0380015191.01.T01:CDS | 40.0% |
| ! | TGCCCTCATTAATGGATCAA+TGG | + | Chr3:66499102-66499121 | MsG0380015191.01.T01:CDS | 40.0% |
| AAAATCAGCAGCCTCAAGAC+AGG | - | Chr3:66499801-66499820 | None:intergenic | 45.0% | |
| ACGACCTCTACCACGAAAAT+GGG | - | Chr3:66499528-66499547 | None:intergenic | 45.0% | |
| GAATGACACTACCTGTCTTG+AGG | + | Chr3:66499787-66499806 | MsG0380015191.01.T01:CDS | 45.0% | |
| GATGCGGAAAGAAAGGTCAT+TGG | + | Chr3:66499666-66499685 | MsG0380015191.01.T01:CDS | 45.0% | |
| GATTCTTGAGGGAGACGATT+TGG | + | Chr3:66498917-66498936 | MsG0380015191.01.T01:CDS | 45.0% | |
| GGATATGCCATTGTTCCTGT+AGG | - | Chr3:66499392-66499411 | None:intergenic | 45.0% | |
| GGGACTAGACAAGATTCTTG+AGG | + | Chr3:66498905-66498924 | MsG0380015191.01.T01:CDS | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | + | Chr3:66499446-66499465 | MsG0380015191.01.T01:CDS | 45.0% | |
| GTCATTGGGCTAAAACATGC+AGG | + | Chr3:66499681-66499700 | MsG0380015191.01.T01:CDS | 45.0% | |
| TATAATCGCCAAAGAGGACG+TGG | + | Chr3:66499452-66499471 | MsG0380015191.01.T01:CDS | 45.0% | |
| TTTGGTTGCAGCTCTTCTAG+TGG | + | Chr3:66499322-66499341 | MsG0380015191.01.T01:CDS | 45.0% | |
| ! | AGAACCCATTTTCGTGGTAG+AGG | + | Chr3:66499521-66499540 | MsG0380015191.01.T01:CDS | 45.0% |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | + | Chr3:66499428-66499447 | MsG0380015191.01.T01:CDS | 45.0% |
| !! | TATTTCTAGAGGGACCTTCC+TGG | - | Chr3:66499628-66499647 | None:intergenic | 45.0% |
| !!! | GAGGCTGCTGATTTTGTTGA+AGG | + | Chr3:66499806-66499825 | MsG0380015191.01.T01:CDS | 45.0% |
| CACGACCTCTACCACGAAAA+TGG | - | Chr3:66499529-66499548 | None:intergenic | 50.0% | |
| CATATCCACCACGTCCTCTT+TGG | - | Chr3:66499463-66499482 | None:intergenic | 50.0% | |
| CTGGCGACAACTACATAACC+TGG | + | Chr3:66498850-66498869 | MsG0380015191.01.T01:CDS | 50.0% | |
| GTTGCGTCCTACAGGAACAA+TGG | + | Chr3:66499382-66499401 | MsG0380015191.01.T01:CDS | 50.0% | |
| TTGTTCCTGTAGGACGCAAC+TGG | - | Chr3:66499382-66499401 | None:intergenic | 50.0% | |
| ! | CATTTTCGTGGTAGAGGTCG+TGG | + | Chr3:66499527-66499546 | MsG0380015191.01.T01:CDS | 50.0% |
| ! | CCACGAAAATGGGTTCTGCT+AGG | - | Chr3:66499518-66499537 | None:intergenic | 50.0% |
| ! | CCTAGCAGAACCCATTTTCG+TGG | + | Chr3:66499515-66499534 | MsG0380015191.01.T01:CDS | 50.0% |
| ! | GGTGGATATGCTCGTTTTGG+TGG | + | Chr3:66499473-66499492 | MsG0380015191.01.T01:CDS | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | + | Chr3:66499479-66499498 | MsG0380015191.01.T01:CDS | 50.0% |
| !!! | CGTGGTGGATATGCTCGTTT+TGG | + | Chr3:66499470-66499489 | MsG0380015191.01.T01:CDS | 50.0% |
| AACCACCAGTTGCGTCCTAC+AGG | + | Chr3:66499374-66499393 | MsG0380015191.01.T01:CDS | 55.0% | |
| AATCGCCAAAGAGGACGTGG+TGG | + | Chr3:66499455-66499474 | MsG0380015191.01.T01:CDS | 55.0% | |
| TTCCTGTAGGACGCAACTGG+TGG | - | Chr3:66499379-66499398 | None:intergenic | 55.0% | |
| ! | CGATTTGGGAGATGTCCCAG+AGG | + | Chr3:66498932-66498951 | MsG0380015191.01.T01:CDS | 55.0% |
| CGTGGTAGAGGTCGTGGAAG+AGG | + | Chr3:66499533-66499552 | MsG0380015191.01.T01:CDS | 60.0% | |
| !! | CGTTTTGGTGGTCGTGGTCG+AGG | + | Chr3:66499485-66499504 | MsG0380015191.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 66498810 | 66499847 | 66498810 | ID=MsG0380015191.01;Name=MsG0380015191.01 |
| Chr3 | mRNA | 66498810 | 66499847 | 66498810 | ID=MsG0380015191.01.T01;Parent=MsG0380015191.01;Name=MsG0380015191.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|345 |
| Chr3 | exon | 66498810 | 66499847 | 66498810 | ID=MsG0380015191.01.T01:exon:23004;Parent=MsG0380015191.01.T01 |
| Chr3 | CDS | 66498810 | 66499847 | 66498810 | ID=MsG0380015191.01.T01:cds;Parent=MsG0380015191.01.T01 |
| Gene Sequence |
| Protein sequence |
