AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012192.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012192.01.T01 MTR_7g062720 40.541 148 80 3 1 146 1 142 6.41e-32 117
MsG0380012192.01.T01 MTR_4g037355 38.686 137 62 3 155 290 1 116 1.96e-21 89.4
MsG0380012192.01.T01 MTR_5g061150 33.333 111 74 0 40 150 26 136 8.77e-18 79.3
MsG0380012192.01.T01 MTR_4g112730 57.971 69 28 1 143 211 2 69 1.38e-17 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 66 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCTTATTGCCCTCATTAA+TGG 0.201947 3:-12435424 MsG0380012192.01.T01:CDS
AAAGTTGTGGCGTTTATTTC+AGG 0.203868 3:+12435108 None:intergenic
TGGATCAGTGGAACAAATTA+AGG 0.229899 3:-12435404 MsG0380012192.01.T01:CDS
CAAAAGATCCCAAGATCTTA+TGG 0.237847 3:-12435482 MsG0380012192.01.T01:CDS
GACAAAATTAAAGCAAGATT+TGG 0.252197 3:-12435459 MsG0380012192.01.T01:CDS
GATTCTTGAGGGAGACGATT+TGG 0.271296 3:-12435601 MsG0380012192.01.T01:CDS
CACGACCTCTACCACGAAAA+TGG 0.272842 3:+12434992 None:intergenic
CTCAATTAAGTTGTTGTTCC+AGG 0.274617 3:+12435650 None:intergenic
TGTTGAAGAAGAATTTAATA+TGG 0.313005 3:-12434698 MsG0380012192.01.T01:CDS
ATTTACTGAATACTCTGATT+TGG 0.325598 3:-12435214 MsG0380012192.01.T01:CDS
GAAGAGGTTATATGAATTAT+AGG 0.339571 3:-12434969 MsG0380012192.01.T01:CDS
GGGAGATGTCCCAGAGGAAT+TGG 0.345733 3:-12435580 MsG0380012192.01.T01:CDS
CTTGATGATTCTTGTTTGAT+TGG 0.348440 3:+12434932 None:intergenic
ATTCTTGAGGGAGACGATTT+GGG 0.354692 3:-12435600 MsG0380012192.01.T01:CDS
AAGACAGGTAGTGTCATTCT+TGG 0.372158 3:+12434735 None:intergenic
GATTTCTAGAGGGACCTTCC+TGG 0.373636 3:+12434893 None:intergenic
CATAGCCACCACGTCCTCTT+TGG 0.374275 3:+12435058 None:intergenic
TATAACTGAAGTAGAAAAGT+TGG 0.390961 3:-12435301 MsG0380012192.01.T01:CDS
CGTGGTGGCTATGCTCGTTT+TGG 0.395091 3:-12435048 MsG0380012192.01.T01:CDS
ACAAAATTAAAGCAAGATTT+GGG 0.407053 3:-12435458 MsG0380012192.01.T01:CDS
AGACAGGTAGTGTCATTCTT+GGG 0.452653 3:+12434736 None:intergenic
AAAAGATCCCAAGATCTTAT+GGG 0.458748 3:-12435481 MsG0380012192.01.T01:CDS
AACCATCAGTTGCGTCCTAC+AGG 0.459311 3:-12435144 MsG0380012192.01.T01:CDS
CGTGGTAGAGGTCGTGGAAG+AGG 0.462783 3:-12434985 MsG0380012192.01.T01:CDS
AACAAGAATCATCAAGGTAA+AGG 0.485600 3:-12434925 MsG0380012192.01.T01:CDS
ATCTGTGGATGGAAAGGAAA+GGG 0.491819 3:-12434782 MsG0380012192.01.T01:CDS
ATCAAGCACCACATTGATGA+TGG 0.492315 3:-12435528 MsG0380012192.01.T01:CDS
CTGGCGACAACTACATAACC+TGG 0.502097 3:-12435668 MsG0380012192.01.T01:CDS
GATGCGGAAAGAAAGGTCAT+TGG 0.503149 3:-12434852 MsG0380012192.01.T01:CDS
ATGCGGAAAGAAAGGTCATT+GGG 0.509313 3:-12434851 MsG0380012192.01.T01:CDS
TGTTATAGATGCGGAAAGAA+AGG 0.510631 3:-12434859 MsG0380012192.01.T01:CDS
GGACTAGACAAGATTCTTGA+GGG 0.521095 3:-12435612 MsG0380012192.01.T01:CDS
TGCAACAACAATATAGAATG+AGG 0.523271 3:-12435239 MsG0380012192.01.T01:CDS
TTTGATTGGTCATATTTGAG+AGG 0.523610 3:+12434946 None:intergenic
CAAAGAATCTGTGGATGGAA+AGG 0.532926 3:-12434788 MsG0380012192.01.T01:CDS
CGTGGACGAGGTAGAAATCA+TGG 0.535808 3:-12435021 MsG0380012192.01.T01:CDS
GTCATTGGGCTAAAGCATGC+AGG 0.540179 3:-12434837 MsG0380012192.01.T01:CDS
CCCTCTAGAAATCATGATGA+TGG 0.541875 3:-12434883 MsG0380012192.01.T01:CDS
GTTCCACTGATCCATTAATG+AGG 0.543612 3:+12435413 None:intergenic
AAAATCAGCAGCTTCAAGAC+AGG 0.547335 3:+12434720 None:intergenic
TCTAGAGGGACCTTCCTGGA+TGG 0.548529 3:+12434897 None:intergenic
TTCCACTGATCCATTAATGA+GGG 0.554709 3:+12435414 None:intergenic
TTCCTGTAGGACGCAACTGA+TGG 0.562606 3:+12435142 None:intergenic
AATTGAGTATCTTGCATGTG+AGG 0.565013 3:-12435634 MsG0380012192.01.T01:CDS
AGGTAAAGGTAAAACCATCC+AGG 0.569963 3:-12434911 MsG0380012192.01.T01:CDS
ATTGAGTATCTTGCATGTGA+GGG 0.575966 3:-12435633 MsG0380012192.01.T01:CDS
GGATATGCCATTGTTCCTGT+AGG 0.578642 3:+12435129 None:intergenic
CAATCAAACAAGAATCATCA+AGG 0.578649 3:-12434931 MsG0380012192.01.T01:CDS
TTTGGTTGCAGCTCTTCTAG+TGG 0.579996 3:-12435196 MsG0380012192.01.T01:CDS
TGCCCTCATTAATGGATCAG+TGG 0.582546 3:-12435416 MsG0380012192.01.T01:CDS
AGATACAAAGAATCTGTGGA+TGG 0.602731 3:-12434793 MsG0380012192.01.T01:CDS
GCAACAACAATATAGAATGA+GGG 0.604762 3:-12435238 MsG0380012192.01.T01:CDS
ACGCCCTTGATTAAAAGTTG+TGG 0.608515 3:+12435095 None:intergenic
TATAATCGCCAAAGAGGACG+TGG 0.608957 3:-12435066 MsG0380012192.01.T01:CDS
GGGACTAGACAAGATTCTTG+AGG 0.613207 3:-12435613 MsG0380012192.01.T01:CDS
ACCATCATCATGATTTCTAG+AGG 0.616492 3:+12434882 None:intergenic
TAAAAGATACAAAGAATCTG+TGG 0.628320 3:-12434797 MsG0380012192.01.T01:CDS
GGTGGTTATAATCGCCAAAG+AGG 0.642086 3:-12435072 MsG0380012192.01.T01:CDS
CCATCATCATGATTTCTAGA+GGG 0.647682 3:+12434883 None:intergenic
GTTGCGTCCTACAGGAACAA+TGG 0.648629 3:-12435136 MsG0380012192.01.T01:CDS
AATCTGTGGATGGAAAGGAA+AGG 0.653361 3:-12434783 MsG0380012192.01.T01:CDS
AAAGGTAAAACCATCCAGGA+AGG 0.653894 3:-12434907 MsG0380012192.01.T01:CDS
GATGATGGTTGTTATAGATG+CGG 0.696569 3:-12434868 MsG0380012192.01.T01:CDS
AATCGCCAAAGAGGACGTGG+TGG 0.698905 3:-12435063 MsG0380012192.01.T01:CDS
TTTGTAATCCATCATCAATG+TGG 0.715751 3:+12435520 None:intergenic
CGATTTGGGAGATGTCCCAG+AGG 0.725435 3:-12435586 MsG0380012192.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAATTAAAGCAAGATTT+GGG - Chr3:12434943-12434962 MsG0380012192.01.T01:CDS 20.0%
!! TGTTGAAGAAGAATTTAATA+TGG - Chr3:12435703-12435722 MsG0380012192.01.T01:CDS 20.0%
!!! TTCAAAATTTTAAGCATAAC+TGG - Chr3:12434714-12434733 MsG0380012192.01.T01:CDS 20.0%
! ATTTACTGAATACTCTGATT+TGG - Chr3:12435187-12435206 MsG0380012192.01.T01:CDS 25.0%
! GAAGAGGTTATATGAATTAT+AGG - Chr3:12435432-12435451 MsG0380012192.01.T01:CDS 25.0%
! GACAAAATTAAAGCAAGATT+TGG - Chr3:12434942-12434961 MsG0380012192.01.T01:CDS 25.0%
! TAAAAGATACAAAGAATCTG+TGG - Chr3:12435604-12435623 MsG0380012192.01.T01:CDS 25.0%
! TATAACTGAAGTAGAAAAGT+TGG - Chr3:12435100-12435119 MsG0380012192.01.T01:CDS 25.0%
!! ATTGCACAATTAGAATTTTG+TGG - Chr3:12435071-12435090 MsG0380012192.01.T01:CDS 25.0%
!!! CAACAACAGTTTTATAATCT+TGG + Chr3:12435025-12435044 None:intergenic 25.0%
AAAAGATCCCAAGATCTTAT+GGG - Chr3:12434920-12434939 MsG0380012192.01.T01:CDS 30.0%
AACAAGAATCATCAAGGTAA+AGG - Chr3:12435476-12435495 MsG0380012192.01.T01:CDS 30.0%
CAATCAAACAAGAATCATCA+AGG - Chr3:12435470-12435489 MsG0380012192.01.T01:CDS 30.0%
GCAACAACAATATAGAATGA+GGG - Chr3:12435163-12435182 MsG0380012192.01.T01:CDS 30.0%
TGCAACAACAATATAGAATG+AGG - Chr3:12435162-12435181 MsG0380012192.01.T01:CDS 30.0%
TTTGTAATCCATCATCAATG+TGG + Chr3:12434884-12434903 None:intergenic 30.0%
! AATTTTGTCCCATAAGATCT+TGG + Chr3:12434931-12434950 None:intergenic 30.0%
! ATTTTGTCCCATAAGATCTT+GGG + Chr3:12434930-12434949 None:intergenic 30.0%
! CTTGATGATTCTTGTTTGAT+TGG + Chr3:12435472-12435491 None:intergenic 30.0%
! TATCTTTTACACAGATACTC+TGG + Chr3:12435593-12435612 None:intergenic 30.0%
! TTTGATTGGTCATATTTGAG+AGG + Chr3:12435458-12435477 None:intergenic 30.0%
AAAGTTGTGGCGTTTATTTC+AGG + Chr3:12435296-12435315 None:intergenic 35.0%
AATTGAGTATCTTGCATGTG+AGG - Chr3:12434767-12434786 MsG0380012192.01.T01:CDS 35.0%
AGATACAAAGAATCTGTGGA+TGG - Chr3:12435608-12435627 MsG0380012192.01.T01:CDS 35.0%
ATTGAGTATCTTGCATGTGA+GGG - Chr3:12434768-12434787 MsG0380012192.01.T01:CDS 35.0%
CAAAAGATCCCAAGATCTTA+TGG - Chr3:12434919-12434938 MsG0380012192.01.T01:CDS 35.0%
TGGATCAGTGGAACAAATTA+AGG - Chr3:12434997-12435016 MsG0380012192.01.T01:CDS 35.0%
TGTTATAGATGCGGAAAGAA+AGG - Chr3:12435542-12435561 MsG0380012192.01.T01:CDS 35.0%
TTCCACTGATCCATTAATGA+GGG + Chr3:12434990-12435009 None:intergenic 35.0%
! AACGCCACAACTTTTAATCA+AGG - Chr3:12435302-12435321 MsG0380012192.01.T01:CDS 35.0%
! ACCATCATCATGATTTCTAG+AGG + Chr3:12435522-12435541 None:intergenic 35.0%
! ACGCCACAACTTTTAATCAA+GGG - Chr3:12435303-12435322 MsG0380012192.01.T01:CDS 35.0%
! CCATCATCATGATTTCTAGA+GGG + Chr3:12435521-12435540 None:intergenic 35.0%
! CTCAATTAAGTTGTTGTTCC+AGG + Chr3:12434754-12434773 None:intergenic 35.0%
! GATGATGGTTGTTATAGATG+CGG - Chr3:12435533-12435552 MsG0380012192.01.T01:CDS 35.0%
! TTTTCTTTGCCAATTCCTCT+GGG + Chr3:12434833-12434852 None:intergenic 35.0%
! TTTTTCTTTGCCAATTCCTC+TGG + Chr3:12434834-12434853 None:intergenic 35.0%
!! AGTCTTATTGCCCTCATTAA+TGG - Chr3:12434977-12434996 MsG0380012192.01.T01:CDS 35.0%
AAAATCAGCAGCTTCAAGAC+AGG + Chr3:12435684-12435703 None:intergenic 40.0%
AAAGGTAAAACCATCCAGGA+AGG - Chr3:12435494-12435513 MsG0380012192.01.T01:CDS 40.0%
AATCTGTGGATGGAAAGGAA+AGG - Chr3:12435618-12435637 MsG0380012192.01.T01:CDS 40.0%
ACGCCCTTGATTAAAAGTTG+TGG + Chr3:12435309-12435328 None:intergenic 40.0%
AGGTAAAGGTAAAACCATCC+AGG - Chr3:12435490-12435509 MsG0380012192.01.T01:CDS 40.0%
ATCAAGCACCACATTGATGA+TGG - Chr3:12434873-12434892 MsG0380012192.01.T01:CDS 40.0%
ATCTGTGGATGGAAAGGAAA+GGG - Chr3:12435619-12435638 MsG0380012192.01.T01:CDS 40.0%
ATGCGGAAAGAAAGGTCATT+GGG - Chr3:12435550-12435569 MsG0380012192.01.T01:CDS 40.0%
ATTCTTGAGGGAGACGATTT+GGG - Chr3:12434801-12434820 MsG0380012192.01.T01:CDS 40.0%
CAAAGAATCTGTGGATGGAA+AGG - Chr3:12435613-12435632 MsG0380012192.01.T01:CDS 40.0%
GGACTAGACAAGATTCTTGA+GGG - Chr3:12434789-12434808 MsG0380012192.01.T01:CDS 40.0%
GTTCCACTGATCCATTAATG+AGG + Chr3:12434991-12435010 None:intergenic 40.0%
! AACTTTTCATGCATCTCAGG+TGG - Chr3:12435136-12435155 MsG0380012192.01.T01:CDS 40.0%
! AAGACAGGTAGTGTCATTCT+TGG + Chr3:12435669-12435688 None:intergenic 40.0%
! ACAACTTTTAATCAAGGGCG+TGG - Chr3:12435308-12435327 MsG0380012192.01.T01:CDS 40.0%
! AGACAGGTAGTGTCATTCTT+GGG + Chr3:12435668-12435687 None:intergenic 40.0%
! CCCTCTAGAAATCATGATGA+TGG - Chr3:12435518-12435537 MsG0380012192.01.T01:CDS 40.0%
! CTCAACTTTTCATGCATCTC+AGG - Chr3:12435133-12435152 MsG0380012192.01.T01:CDS 40.0%
GATGCGGAAAGAAAGGTCAT+TGG - Chr3:12435549-12435568 MsG0380012192.01.T01:CDS 45.0%
GATTCTTGAGGGAGACGATT+TGG - Chr3:12434800-12434819 MsG0380012192.01.T01:CDS 45.0%
GGATATGCCATTGTTCCTGT+AGG + Chr3:12435275-12435294 None:intergenic 45.0%
GGGACTAGACAAGATTCTTG+AGG - Chr3:12434788-12434807 MsG0380012192.01.T01:CDS 45.0%
GGTGGTTATAATCGCCAAAG+AGG - Chr3:12435329-12435348 MsG0380012192.01.T01:CDS 45.0%
TATAATCGCCAAAGAGGACG+TGG - Chr3:12435335-12435354 MsG0380012192.01.T01:CDS 45.0%
TTTGGTTGCAGCTCTTCTAG+TGG - Chr3:12435205-12435224 MsG0380012192.01.T01:CDS 45.0%
! CATGGTCGAAACCATTTTCG+TGG - Chr3:12435398-12435417 MsG0380012192.01.T01:CDS 45.0%
! CGAAACCATTTTCGTGGTAG+AGG - Chr3:12435404-12435423 MsG0380012192.01.T01:CDS 45.0%
! TGCCCTCATTAATGGATCAG+TGG - Chr3:12434985-12435004 MsG0380012192.01.T01:CDS 45.0%
!! ACTTTTAATCAAGGGCGTGG+TGG - Chr3:12435311-12435330 MsG0380012192.01.T01:CDS 45.0%
AACCATCAGTTGCGTCCTAC+AGG - Chr3:12435257-12435276 MsG0380012192.01.T01:CDS 50.0%
CACGACCTCTACCACGAAAA+TGG + Chr3:12435412-12435431 None:intergenic 50.0%
CGTGGACGAGGTAGAAATCA+TGG - Chr3:12435380-12435399 MsG0380012192.01.T01:CDS 50.0%
CTGGCGACAACTACATAACC+TGG - Chr3:12434733-12434752 MsG0380012192.01.T01:CDS 50.0%
GTCATTGGGCTAAAGCATGC+AGG - Chr3:12435564-12435583 MsG0380012192.01.T01:CDS 50.0%
GTTGCGTCCTACAGGAACAA+TGG - Chr3:12435265-12435284 MsG0380012192.01.T01:CDS 50.0%
TTCCTGTAGGACGCAACTGA+TGG + Chr3:12435262-12435281 None:intergenic 50.0%
! CATTTTCGTGGTAGAGGTCG+TGG - Chr3:12435410-12435429 MsG0380012192.01.T01:CDS 50.0%
! GATTTCTAGAGGGACCTTCC+TGG + Chr3:12435511-12435530 None:intergenic 50.0%
!! TATGCTCGTTTTGGTGGTCG+TGG - Chr3:12435362-12435381 MsG0380012192.01.T01:CDS 50.0%
AATCGCCAAAGAGGACGTGG+TGG - Chr3:12435338-12435357 MsG0380012192.01.T01:CDS 55.0%
CATAGCCACCACGTCCTCTT+TGG + Chr3:12435346-12435365 None:intergenic 55.0%
GGGAGATGTCCCAGAGGAAT+TGG - Chr3:12434821-12434840 MsG0380012192.01.T01:CDS 55.0%
TCTAGAGGGACCTTCCTGGA+TGG + Chr3:12435507-12435526 None:intergenic 55.0%
! CGATTTGGGAGATGTCCCAG+AGG - Chr3:12434815-12434834 MsG0380012192.01.T01:CDS 55.0%
! CGTGGTGGCTATGCTCGTTT+TGG - Chr3:12435353-12435372 MsG0380012192.01.T01:CDS 55.0%
! GGTGGCTATGCTCGTTTTGG+TGG - Chr3:12435356-12435375 MsG0380012192.01.T01:CDS 55.0%
CGTGGTAGAGGTCGTGGAAG+AGG - Chr3:12435416-12435435 MsG0380012192.01.T01:CDS 60.0%
!! CGTTTTGGTGGTCGTGGACG+AGG - Chr3:12435368-12435387 MsG0380012192.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 12434693 12435730 12434693 ID=MsG0380012192.01;Name=MsG0380012192.01
Chr3 mRNA 12434693 12435730 12434693 ID=MsG0380012192.01.T01;Parent=MsG0380012192.01;Name=MsG0380012192.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|345
Chr3 exon 12434693 12435730 12434693 ID=MsG0380012192.01.T01:exon:2394;Parent=MsG0380012192.01.T01
Chr3 CDS 12434693 12435730 12434693 ID=MsG0380012192.01.T01:cds;Parent=MsG0380012192.01.T01
Gene Sequence

>MsG0380012192.01.T01

ATGTCAAACGTTAAGCATCAATTCAAAATTTTAAGCATAACTGGCGACAACTACATAACCTGGAACAACAACTTAATTGAGTATCTTGCATGTGAGGGACTAGACAAGATTCTTGAGGGAGACGATTTGGGAGATGTCCCAGAGGAATTGGCAAAGAAAAAATCAAAAGTAAATCGTATAATCAAGCACCACATTGATGATGGATTACAAACAGAGTATTCAAATGCAAAAGATCCCAAGATCTTATGGGACAAAATTAAAGCAAGATTTGGGCATCAAAAGAAAGTCTTATTGCCCTCATTAATGGATCAGTGGAACAAATTAAGGTTCCAAGATTATAAAACTGTTGTTGCATATAACTCTGCTATGCATCAAATTATTGCACAATTAGAATTTTGTGGAAAAAATATAACTGAAGTAGAAAAGTTGGAGAAAACATTCTCAACTTTTCATGCATCTCAGGTGGTGTTGCAACAACAATATAGAATGAGGGAATTTACTGAATACTCTGATTTGGTTGCAGCTCTTCTAGTGGCTGAACAAAATAACGAGCTGCTCATTAAAAACCATCAGTTGCGTCCTACAGGAACAATGGCATATCCTGAAATAAACGCCACAACTTTTAATCAAGGGCGTGGTGGTTATAATCGCCAAAGAGGACGTGGTGGCTATGCTCGTTTTGGTGGTCGTGGACGAGGTAGAAATCATGGTCGAAACCATTTTCGTGGTAGAGGTCGTGGAAGAGGTTATATGAATTATAGGCCTCTCAAATATGACCAATCAAACAAGAATCATCAAGGTAAAGGTAAAACCATCCAGGAAGGTCCCTCTAGAAATCATGATGATGGTTGTTATAGATGCGGAAAGAAAGGTCATTGGGCTAAAGCATGCAGGACACCAGAGTATCTGTGTAAAAGATACAAAGAATCTGTGGATGGAAAGGAAAGGGAAGTTAATTTTAATGAAATTAAACCCAAGAATGACACTACCTGTCTTGAAGCTGCTGATTTTGTTGAAGAAGAATTTAATATGGATTAA

Protein sequence

>MsG0380012192.01.T01

MSNVKHQFKILSITGDNYITWNNNLIEYLACEGLDKILEGDDLGDVPEELAKKKSKVNRIIKHHIDDGLQTEYSNAKDPKILWDKIKARFGHQKKVLLPSLMDQWNKLRFQDYKTVVAYNSAMHQIIAQLEFCGKNITEVEKLEKTFSTFHASQVVLQQQYRMREFTEYSDLVAALLVAEQNNELLIKNHQLRPTGTMAYPEINATTFNQGRGGYNRQRGRGGYARFGGRGRGRNHGRNHFRGRGRGRGYMNYRPLKYDQSNKNHQGKGKTIQEGPSRNHDDGCYRCGKKGHWAKACRTPEYLCKRYKESVDGKEREVNFNEIKPKNDTTCLEAADFVEEEFNMD*