Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015757.01.T01 | KEH35027.1 | 96.552 | 116 | 4 | 0 | 1 | 116 | 1 | 116 | 2.29E-75 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015757.01.T01 | A0A072UZN8 | 96.552 | 116 | 4 | 0 | 1 | 116 | 1 | 116 | 1.09e-75 | 229 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003274.01 | MsG0380015757.01 | 0.814332 | 1.625933e-51 | 1.087874e-48 |
| MsG0380015757.01 | MsG0380015758.01 | 0.867273 | 1.544919e-65 | 5.361793e-62 |
| MsG0380015757.01 | MsG0580026024.01 | 0.803565 | 3.280638e-49 | 1.654105e-46 |
| MsG0380015757.01 | MsG0780037963.01 | 0.815631 | 8.375422e-52 | 5.805428e-49 |
| MsG0380015757.01 | MsG0880044453.01 | 0.805848 | 1.094701e-49 | 5.853230e-47 |
| MsG0380015757.01 | MsG0880047660.01 | 0.800176 | 1.629847e-48 | 7.538346e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015757.01.T01 | MTR_3g076950 | 96.552 | 116 | 4 | 0 | 1 | 116 | 1 | 116 | 2.77e-79 | 229 |
| MsG0380015757.01.T01 | MTR_3g076950 | 96.552 | 116 | 4 | 0 | 1 | 116 | 1 | 116 | 6.72e-79 | 228 |
| MsG0380015757.01.T01 | MTR_3g076960 | 53.448 | 116 | 45 | 4 | 1 | 115 | 1 | 108 | 4.63e-25 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTACCTCTCCCACATTTC+AGG | 0.262487 | 3:-74706682 | MsG0380015757.01.T01:CDS |
| TTGAAGGCCATCTCGGCATT+TGG | 0.273710 | 3:+74706616 | None:intergenic |
| TGGTGGTGGTGATGGAGGCC+AGG | 0.325123 | 3:+74706553 | None:intergenic |
| TGCAGAAATGGAAGACATTC+AGG | 0.354752 | 3:-74706375 | MsG0380015757.01.T01:CDS |
| CAGGGTTGTGGTGGTGGTGA+TGG | 0.386204 | 3:+74706545 | None:intergenic |
| TGAAGGCCATCTCGGCATTT+GGG | 0.408131 | 3:+74706617 | None:intergenic |
| GTGGTCTTGAATTGCAGAAA+TGG | 0.416224 | 3:-74706387 | MsG0380015757.01.T01:CDS |
| GGTGGTGGTGATGGAGGCCA+GGG | 0.441175 | 3:+74706554 | None:intergenic |
| ATACATAACCCTCTTGTTGA+AGG | 0.453856 | 3:+74706600 | None:intergenic |
| GGATCACAGGGTTGTGGTGG+TGG | 0.468374 | 3:+74706539 | None:intergenic |
| GGTTGTGGTGGTGGTGATGG+AGG | 0.469628 | 3:+74706548 | None:intergenic |
| CAACAAGAGGGTTATGTATG+AGG | 0.473099 | 3:-74706596 | MsG0380015757.01.T01:CDS |
| TCAACCCTCTCACGGATCAC+AGG | 0.488304 | 3:+74706526 | None:intergenic |
| ATCTCGGCATTTGGGTAGCG+AGG | 0.500298 | 3:+74706625 | None:intergenic |
| CCTCTTGTTGAAGGCCATCT+CGG | 0.506710 | 3:+74706609 | None:intergenic |
| GATGAGATCGAGAGATCCCC+TGG | 0.509719 | 3:-74706571 | MsG0380015757.01.T01:CDS |
| CCCTTCTGACACCTGAAATG+TGG | 0.510434 | 3:+74706671 | None:intergenic |
| ATGAACGAGTGCCTGAGGTT+AGG | 0.516029 | 3:-74706492 | MsG0380015757.01.T01:CDS |
| CCACCTGGTCTATTCCTTCG+TGG | 0.525594 | 3:+74706406 | None:intergenic |
| TTCTGCAATTCAAGACCACC+TGG | 0.539379 | 3:+74706391 | None:intergenic |
| TCGCTACCCAAATGCCGAGA+TGG | 0.540986 | 3:-74706623 | MsG0380015757.01.T01:CDS |
| CCCACATTTCAGGTGTCAGA+AGG | 0.548322 | 3:-74706672 | MsG0380015757.01.T01:CDS |
| CCTTCTGACACCTGAAATGT+GGG | 0.553733 | 3:+74706672 | None:intergenic |
| GTGGTGGTGATGGAGGCCAG+GGG | 0.559067 | 3:+74706555 | None:intergenic |
| GGGACGTCGGTGAGAATACT+CGG | 0.567594 | 3:+74706646 | None:intergenic |
| CCACCACTTCAACCCTCTCA+CGG | 0.569930 | 3:+74706518 | None:intergenic |
| CCGTGAGAGGGTTGAAGTGG+TGG | 0.574338 | 3:-74706518 | MsG0380015757.01.T01:CDS |
| CACGGATCACAGGGTTGTGG+TGG | 0.575880 | 3:+74706536 | None:intergenic |
| CAACCCTCTCACGGATCACA+GGG | 0.582696 | 3:+74706527 | None:intergenic |
| GTGATCTATGAAATCGAAGA+GGG | 0.592242 | 3:-74706457 | MsG0380015757.01.T01:CDS |
| TGACACCTGAAATGTGGGAG+AGG | 0.593532 | 3:+74706677 | None:intergenic |
| TTATCACCATACCTAACCTC+AGG | 0.596763 | 3:+74706481 | None:intergenic |
| CCGAGATGGCCTTCAACAAG+AGG | 0.602505 | 3:-74706609 | MsG0380015757.01.T01:CDS |
| TCTCACGGATCACAGGGTTG+TGG | 0.603361 | 3:+74706533 | None:intergenic |
| CCACATTTCAGGTGTCAGAA+GGG | 0.607175 | 3:-74706671 | MsG0380015757.01.T01:CDS |
| CGAGTGCCTGAGGTTAGGTA+TGG | 0.623840 | 3:-74706487 | MsG0380015757.01.T01:CDS |
| AGAGATCATGTTGAGATCCG+AGG | 0.635203 | 3:-74706704 | None:intergenic |
| ACAACCCTGTGATCCGTGAG+AGG | 0.642119 | 3:-74706531 | MsG0380015757.01.T01:CDS |
| CGAGATGGCCTTCAACAAGA+GGG | 0.643622 | 3:-74706608 | MsG0380015757.01.T01:CDS |
| CAACCCTGTGATCCGTGAGA+GGG | 0.649779 | 3:-74706530 | MsG0380015757.01.T01:CDS |
| TCTCGGCATTTGGGTAGCGA+GGG | 0.649823 | 3:+74706626 | None:intergenic |
| AATGTGGGAGAGGTAGTCCT+CGG | 0.653733 | 3:+74706687 | None:intergenic |
| AGTGATCTATGAAATCGAAG+AGG | 0.656153 | 3:-74706458 | MsG0380015757.01.T01:CDS |
| CCAATCAAAATTATCCACGA+AGG | 0.659783 | 3:-74706420 | MsG0380015757.01.T01:CDS |
| TATCCACGAAGGAATAGACC+AGG | 0.660548 | 3:-74706409 | MsG0380015757.01.T01:CDS |
| ATTTGGGTAGCGAGGGACGT+CGG | 0.663123 | 3:+74706633 | None:intergenic |
| GATCCGTGAGAGGGTTGAAG+TGG | 0.674987 | 3:-74706521 | MsG0380015757.01.T01:CDS |
| GGAGTATGAACGAGTGCCTG+AGG | 0.699247 | 3:-74706497 | MsG0380015757.01.T01:CDS |
| CCACGAAGGAATAGACCAGG+TGG | 0.728780 | 3:-74706406 | MsG0380015757.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTGATCTATGAAATCGAAG+AGG | - | Chr3:74706608-74706627 | MsG0380015757.01.T01:CDS | 35.0% | |
| ATACATAACCCTCTTGTTGA+AGG | + | Chr3:74706469-74706488 | None:intergenic | 35.0% | |
| CCAATCAAAATTATCCACGA+AGG | - | Chr3:74706646-74706665 | MsG0380015757.01.T01:CDS | 35.0% | |
| GTGATCTATGAAATCGAAGA+GGG | - | Chr3:74706609-74706628 | MsG0380015757.01.T01:CDS | 35.0% | |
| !! | CCTTCGTGGATAATTTTGAT+TGG | + | Chr3:74706649-74706668 | None:intergenic | 35.0% |
| CAACAAGAGGGTTATGTATG+AGG | - | Chr3:74706470-74706489 | MsG0380015757.01.T01:CDS | 40.0% | |
| TGCAGAAATGGAAGACATTC+AGG | - | Chr3:74706691-74706710 | MsG0380015757.01.T01:CDS | 40.0% | |
| TTATCACCATACCTAACCTC+AGG | + | Chr3:74706588-74706607 | None:intergenic | 40.0% | |
| ! | GTGGTCTTGAATTGCAGAAA+TGG | - | Chr3:74706679-74706698 | MsG0380015757.01.T01:CDS | 40.0% |
| CCTTCTGACACCTGAAATGT+GGG | + | Chr3:74706397-74706416 | None:intergenic | 45.0% | |
| TATCCACGAAGGAATAGACC+AGG | - | Chr3:74706657-74706676 | MsG0380015757.01.T01:CDS | 45.0% | |
| TTCTGCAATTCAAGACCACC+TGG | + | Chr3:74706678-74706697 | None:intergenic | 45.0% | |
| ! | CCACATTTCAGGTGTCAGAA+GGG | - | Chr3:74706395-74706414 | MsG0380015757.01.T01:CDS | 45.0% |
| AATGTGGGAGAGGTAGTCCT+CGG | + | Chr3:74706382-74706401 | None:intergenic | 50.0% | |
| ATGAACGAGTGCCTGAGGTT+AGG | - | Chr3:74706574-74706593 | MsG0380015757.01.T01:CDS | 50.0% | |
| CCCTTCTGACACCTGAAATG+TGG | + | Chr3:74706398-74706417 | None:intergenic | 50.0% | |
| CCTCTTGTTGAAGGCCATCT+CGG | + | Chr3:74706460-74706479 | None:intergenic | 50.0% | |
| CGAGATGGCCTTCAACAAGA+GGG | - | Chr3:74706458-74706477 | MsG0380015757.01.T01:CDS | 50.0% | |
| GACTACCTCTCCCACATTTC+AGG | - | Chr3:74706384-74706403 | MsG0380015757.01.T01:CDS | 50.0% | |
| TGAAGGCCATCTCGGCATTT+GGG | + | Chr3:74706452-74706471 | None:intergenic | 50.0% | |
| TGACACCTGAAATGTGGGAG+AGG | + | Chr3:74706392-74706411 | None:intergenic | 50.0% | |
| TTGAAGGCCATCTCGGCATT+TGG | + | Chr3:74706453-74706472 | None:intergenic | 50.0% | |
| ! | CCCACATTTCAGGTGTCAGA+AGG | - | Chr3:74706394-74706413 | MsG0380015757.01.T01:CDS | 50.0% |
| ACAACCCTGTGATCCGTGAG+AGG | - | Chr3:74706535-74706554 | MsG0380015757.01.T01:CDS | 55.0% | |
| ATCTCGGCATTTGGGTAGCG+AGG | + | Chr3:74706444-74706463 | None:intergenic | 55.0% | |
| ATTTGGGTAGCGAGGGACGT+CGG | + | Chr3:74706436-74706455 | None:intergenic | 55.0% | |
| CAACCCTCTCACGGATCACA+GGG | + | Chr3:74706542-74706561 | None:intergenic | 55.0% | |
| CAACCCTGTGATCCGTGAGA+GGG | - | Chr3:74706536-74706555 | MsG0380015757.01.T01:CDS | 55.0% | |
| CCACCACTTCAACCCTCTCA+CGG | + | Chr3:74706551-74706570 | None:intergenic | 55.0% | |
| CCACCTGGTCTATTCCTTCG+TGG | + | Chr3:74706663-74706682 | None:intergenic | 55.0% | |
| CCACGAAGGAATAGACCAGG+TGG | - | Chr3:74706660-74706679 | MsG0380015757.01.T01:CDS | 55.0% | |
| CCGAGATGGCCTTCAACAAG+AGG | - | Chr3:74706457-74706476 | MsG0380015757.01.T01:CDS | 55.0% | |
| CGAGTGCCTGAGGTTAGGTA+TGG | - | Chr3:74706579-74706598 | MsG0380015757.01.T01:CDS | 55.0% | |
| GATCCGTGAGAGGGTTGAAG+TGG | - | Chr3:74706545-74706564 | MsG0380015757.01.T01:CDS | 55.0% | |
| GATGAGATCGAGAGATCCCC+TGG | - | Chr3:74706495-74706514 | MsG0380015757.01.T01:CDS | 55.0% | |
| GGAGTATGAACGAGTGCCTG+AGG | - | Chr3:74706569-74706588 | MsG0380015757.01.T01:CDS | 55.0% | |
| GGGACGTCGGTGAGAATACT+CGG | + | Chr3:74706423-74706442 | None:intergenic | 55.0% | |
| TCAACCCTCTCACGGATCAC+AGG | + | Chr3:74706543-74706562 | None:intergenic | 55.0% | |
| TCGCTACCCAAATGCCGAGA+TGG | - | Chr3:74706443-74706462 | MsG0380015757.01.T01:CDS | 55.0% | |
| TCTCACGGATCACAGGGTTG+TGG | + | Chr3:74706536-74706555 | None:intergenic | 55.0% | |
| TCTCGGCATTTGGGTAGCGA+GGG | + | Chr3:74706443-74706462 | None:intergenic | 55.0% | |
| CACGGATCACAGGGTTGTGG+TGG | + | Chr3:74706533-74706552 | None:intergenic | 60.0% | |
| CCGTGAGAGGGTTGAAGTGG+TGG | - | Chr3:74706548-74706567 | MsG0380015757.01.T01:CDS | 60.0% | |
| GGATCACAGGGTTGTGGTGG+TGG | + | Chr3:74706530-74706549 | None:intergenic | 60.0% | |
| !! | CAGGGTTGTGGTGGTGGTGA+TGG | + | Chr3:74706524-74706543 | None:intergenic | 60.0% |
| !! | GGTTGTGGTGGTGGTGATGG+AGG | + | Chr3:74706521-74706540 | None:intergenic | 60.0% |
| !! | GGTGGTGGTGATGGAGGCCA+GGG | + | Chr3:74706515-74706534 | None:intergenic | 65.0% |
| !! | GTGGTGGTGATGGAGGCCAG+GGG | + | Chr3:74706514-74706533 | None:intergenic | 65.0% |
| !! | TGGTGGTGGTGATGGAGGCC+AGG | + | Chr3:74706516-74706535 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 74706369 | 74706719 | 74706369 | ID=MsG0380015757.01;Name=MsG0380015757.01 |
| Chr3 | mRNA | 74706369 | 74706719 | 74706369 | ID=MsG0380015757.01.T01;Parent=MsG0380015757.01;Name=MsG0380015757.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|116 |
| Chr3 | exon | 74706369 | 74706719 | 74706369 | ID=MsG0380015757.01.T01:exon:14937;Parent=MsG0380015757.01.T01 |
| Chr3 | CDS | 74706369 | 74706719 | 74706369 | ID=MsG0380015757.01.T01:cds;Parent=MsG0380015757.01.T01 |
| Gene Sequence |
| Protein sequence |
