AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026024.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026024.01.T01 MTR_5g037890 97.619 126 3 0 1 126 1 126 7.23e-92 261
MsG0580026024.01.T01 MTR_5g037950 66.667 126 41 1 1 125 1 126 2.69e-52 161
MsG0580026024.01.T01 MTR_5g037930 66.406 128 41 1 1 126 1 128 3.74e-52 161
MsG0580026024.01.T01 MTR_7g009070 57.547 106 45 0 20 125 28 133 3.46e-44 141
MsG0580026024.01.T01 MTR_3g112410 47.748 111 58 0 14 124 2 112 2.13e-36 120
MsG0580026024.01.T01 MTR_1g023140 43.363 113 64 0 14 126 2 114 1.23e-34 116
MsG0580026024.01.T01 MTR_5g021180 45.045 111 61 0 14 124 4 114 1.90e-34 116
MsG0580026024.01.T01 MTR_8g107460 42.308 104 60 0 18 121 254 357 9.44e-25 96.7
MsG0580026024.01.T01 MTR_8g005320 34.286 105 69 0 17 121 25 129 2.54e-23 88.2
MsG0580026024.01.T01 MTR_7g073680 36.190 105 67 0 17 121 23 127 3.37e-23 88.2
MsG0580026024.01.T01 MTR_2g010750 39.048 105 64 0 18 122 9 113 5.71e-23 87.0
MsG0580026024.01.T01 MTR_8g006685 34.286 105 69 0 17 121 94 198 4.20e-22 87.0
MsG0580026024.01.T01 MTR_8g006560 34.286 105 69 0 17 121 94 198 4.20e-22 87.0
MsG0580026024.01.T01 MTR_4g081380 37.383 107 63 2 18 122 10 114 5.13e-21 82.0
MsG0580026024.01.T01 MTR_2g082590 32.381 105 71 0 18 122 10 114 8.71e-20 79.0
MsG0580026024.01.T01 MTR_2g082590 33.628 113 66 2 10 122 24 127 1.42e-19 78.6
MsG0580026024.01.T01 MTR_1g098660 33.721 86 54 2 39 123 77 160 1.77e-15 68.9
MsG0580026024.01.T01 MTR_7g080250 37.500 88 54 1 40 126 94 181 5.51e-15 67.8
MsG0580026024.01.T01 MTR_4g094690 34.146 82 52 1 44 123 85 166 3.63e-14 65.5
MsG0580026024.01.T01 MTR_8g059015 34.091 88 56 2 38 123 87 174 5.40e-14 65.1
MsG0580026024.01.T01 MTR_3g089065 31.313 99 63 3 27 123 76 171 1.43e-13 63.9
MsG0580026024.01.T01 MTR_2g079420 34.091 88 56 2 38 123 84 171 4.01e-13 62.8
MsG0580026024.01.T01 MTR_1g114290 31.646 79 50 2 38 115 84 159 7.47e-12 59.7
MsG0580026024.01.T01 MTR_3g112400 32.000 100 48 3 15 114 2 81 2.76e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026024.01.T01 AT5G39950 48.649 111 57 0 15 125 22 132 6.07e-42 135
MsG0580026024.01.T01 AT1G69880 47.664 107 56 0 17 123 36 142 5.77e-35 118
MsG0580026024.01.T01 AT1G59730 41.406 128 72 1 1 125 1 128 6.26e-35 117
MsG0580026024.01.T01 AT3G51030 42.727 110 63 0 14 123 2 111 2.85e-33 112
MsG0580026024.01.T01 AT1G19730 46.429 112 59 1 14 124 2 113 1.38e-32 111
MsG0580026024.01.T01 AT1G45145 43.925 107 60 0 18 124 5 111 2.17e-31 108
MsG0580026024.01.T01 AT5G42980 44.037 109 61 0 18 126 5 113 7.94e-31 107
MsG0580026024.01.T01 AT3G17880 40.777 103 61 0 19 121 264 366 1.45e-22 91.3
MsG0580026024.01.T01 AT3G17880 40.777 103 61 0 19 121 271 373 1.46e-22 91.3
MsG0580026024.01.T01 AT3G56420 38.043 92 57 0 28 119 50 141 2.87e-21 83.6
MsG0580026024.01.T01 AT3G56420 38.043 92 57 0 28 119 50 141 2.87e-21 83.6
MsG0580026024.01.T01 AT3G56420 38.043 92 57 0 28 119 50 141 2.87e-21 83.6
MsG0580026024.01.T01 AT3G08710 33.333 111 72 1 11 121 18 126 1.50e-20 81.3
MsG0580026024.01.T01 AT3G08710 33.333 111 72 1 11 121 18 126 1.50e-20 81.3
MsG0580026024.01.T01 AT3G08710 33.333 111 72 1 11 121 18 126 1.50e-20 81.3
MsG0580026024.01.T01 AT1G11530 34.286 105 69 0 18 122 2 106 5.30e-19 76.6
MsG0580026024.01.T01 AT2G40790 34.783 92 60 0 28 119 50 141 3.95e-17 72.8
MsG0580026024.01.T01 AT2G40790 34.783 92 60 0 28 119 50 141 3.95e-17 72.8
MsG0580026024.01.T01 AT5G16400 38.636 88 53 1 40 126 97 184 2.95e-16 71.2
MsG0580026024.01.T01 AT1G43560 29.915 117 77 3 1 112 33 149 3.01e-16 70.9
MsG0580026024.01.T01 AT3G02730 40.230 87 51 1 41 126 88 174 5.61e-16 70.5
MsG0580026024.01.T01 AT4G03520 40.845 71 41 1 43 112 101 171 4.02e-15 68.6
MsG0580026024.01.T01 AT1G76760 30.526 95 59 3 33 120 70 164 1.44e-14 66.6
MsG0580026024.01.T01 AT1G03680 38.272 81 48 2 45 123 97 177 1.79e-14 66.6
MsG0580026024.01.T01 AT3G15360 32.967 91 53 3 38 123 102 189 2.72e-14 66.2
MsG0580026024.01.T01 AT2G35010 37.349 83 48 2 44 123 110 191 2.40e-13 63.9
MsG0580026024.01.T01 AT2G35010 37.349 83 48 2 44 123 110 191 2.40e-13 63.9
MsG0580026024.01.T01 AT1G31020 30.000 110 75 1 16 123 47 156 2.58e-13 63.2
MsG0580026024.01.T01 AT1G52990 34.066 91 58 2 34 123 222 311 2.95e-12 62.4
MsG0580026024.01.T01 AT1G50320 35.443 79 47 2 38 115 85 160 4.96e-12 60.1

Find 24 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCATTCCACTGCTAAA+TGG 0.238150 5:-29311291 MsG0580026024.01.T01:CDS
GCTTGATGTTGATGAGTTAT+TGG 0.264813 5:-29311039 MsG0580026024.01.T01:intron
GCAGCAAAATCTTTCAAAAC+TGG 0.287096 5:+29311089 None:intergenic
TTATTGATTTCACAGCTAAA+TGG 0.322109 5:-29311136 MsG0580026024.01.T01:CDS
GGGAGTGTCTCAAACCTTCC+AGG 0.392325 5:-29310877 MsG0580026024.01.T01:CDS
TAAAGTGGTGGGAGCAAGAA+AGG 0.432390 5:-29310805 MsG0580026024.01.T01:CDS
TTCTTAATGAGTAGAAAAGT+TGG 0.438267 5:+29310843 None:intergenic
GTGTGGACCTTGCAAAGTTA+TGG 0.487071 5:-29311114 MsG0580026024.01.T01:CDS
AGGAAAGGTTGTTGATAAAG+TGG 0.493130 5:-29310820 MsG0580026024.01.T01:CDS
TAGCAGTGGAATGGAATGTA+AGG 0.498154 5:+29311296 None:intergenic
TCTACTCATTAAGAAAGGAA+AGG 0.517021 5:-29310835 MsG0580026024.01.T01:CDS
ATACACAAAATTATACAAGT+TGG 0.527315 5:+29311348 None:intergenic
CATTCCATTTAGCAGTGGAA+TGG 0.530623 5:+29311287 None:intergenic
TGGAATGGAATGTAAGGACA+TGG 0.568326 5:+29311302 None:intergenic
AAGTTGGCAATGAATGCACC+TGG 0.579245 5:+29310859 None:intergenic
TGGCAATGAATGCACCTGGA+AGG 0.589602 5:+29310863 None:intergenic
GATTTCACAGCTAAATGGTG+TGG 0.590907 5:-29311131 MsG0580026024.01.T01:CDS
AAGGTTGTTGATAAAGTGGT+GGG 0.601867 5:-29310816 MsG0580026024.01.T01:CDS
ACTGGATCCATAACTTTGCA+AGG 0.608925 5:+29311107 None:intergenic
ATGAGCATTCCATTTAGCAG+TGG 0.609708 5:+29311282 None:intergenic
AAAGGTTGTTGATAAAGTGG+TGG 0.610487 5:-29310817 MsG0580026024.01.T01:CDS
CAAGAAAGGAGGAACTACAA+AGG 0.696570 5:-29310791 MsG0580026024.01.T01:CDS
AGTGGTGGGAGCAAGAAAGG+AGG 0.703427 5:-29310802 MsG0580026024.01.T01:CDS
GGAATGGAATGTAAGGACAT+GGG 0.736550 5:+29311303 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAAGAAACTAATAAAC+TGG - Chr5:29310871-29310890 MsG0580026024.01.T01:CDS 15.0%
!! ATACACAAAATTATACAAGT+TGG + Chr5:29310777-29310796 None:intergenic 20.0%
!!! TCTTATGCATTATTTTTCTT+TGG - Chr5:29310954-29310973 MsG0580026024.01.T01:intron 20.0%
!!! TTATTTTTCTTTGGTATAGA+TGG - Chr5:29310963-29310982 MsG0580026024.01.T01:intron 20.0%
! TAACAAACTAGTTAAAGTGA+AGG + Chr5:29311124-29311143 None:intergenic 25.0%
! TTCTTAATGAGTAGAAAAGT+TGG + Chr5:29311282-29311301 None:intergenic 25.0%
!! ACTTTTCTACTCATTAAGAA+AGG - Chr5:29311282-29311301 MsG0580026024.01.T01:CDS 25.0%
!! TGTTTTTCCCACAATATAAT+TGG - Chr5:29311217-29311236 MsG0580026024.01.T01:intron 25.0%
!!! TTATTGATTTCACAGCTAAA+TGG - Chr5:29310986-29311005 MsG0580026024.01.T01:intron 25.0%
TCTACTCATTAAGAAAGGAA+AGG - Chr5:29311287-29311306 MsG0580026024.01.T01:CDS 30.0%
CCACAATATAATTGGCAACA+GGG - Chr5:29311225-29311244 MsG0580026024.01.T01:intron 35.0%
CCTGTTGCCAATTATATTGT+GGG + Chr5:29311227-29311246 None:intergenic 35.0%
GCAGCAAAATCTTTCAAAAC+TGG + Chr5:29311036-29311055 None:intergenic 35.0%
! AAAGGTTGTTGATAAAGTGG+TGG - Chr5:29311305-29311324 MsG0580026024.01.T01:CDS 35.0%
! AAGGTTGTTGATAAAGTGGT+GGG - Chr5:29311306-29311325 MsG0580026024.01.T01:CDS 35.0%
! AGGAAAGGTTGTTGATAAAG+TGG - Chr5:29311302-29311321 MsG0580026024.01.T01:CDS 35.0%
! GCTTGATGTTGATGAGTTAT+TGG - Chr5:29311083-29311102 MsG0580026024.01.T01:CDS 35.0%
ACAAATCAGTTCTCAAGTGC+TGG + Chr5:29311200-29311219 None:intergenic 40.0%
ACTGGATCCATAACTTTGCA+AGG + Chr5:29311018-29311037 None:intergenic 40.0%
ATGAGCATTCCATTTAGCAG+TGG + Chr5:29310843-29310862 None:intergenic 40.0%
CAAGAAAGGAGGAACTACAA+AGG - Chr5:29311331-29311350 MsG0580026024.01.T01:CDS 40.0%
CATTCCATTCCACTGCTAAA+TGG - Chr5:29310831-29310850 MsG0580026024.01.T01:CDS 40.0%
CATTCCATTTAGCAGTGGAA+TGG + Chr5:29310838-29310857 None:intergenic 40.0%
CCCACAATATAATTGGCAAC+AGG - Chr5:29311224-29311243 MsG0580026024.01.T01:intron 40.0%
CCCTGTTGCCAATTATATTG+TGG + Chr5:29311228-29311247 None:intergenic 40.0%
GATTTCACAGCTAAATGGTG+TGG - Chr5:29310991-29311010 MsG0580026024.01.T01:intron 40.0%
GGAATGGAATGTAAGGACAT+GGG + Chr5:29310822-29310841 None:intergenic 40.0%
TAGCAGTGGAATGGAATGTA+AGG + Chr5:29310829-29310848 None:intergenic 40.0%
TGGAATGGAATGTAAGGACA+TGG + Chr5:29310823-29310842 None:intergenic 40.0%
AAGTTGGCAATGAATGCACC+TGG + Chr5:29311266-29311285 None:intergenic 45.0%
GTGTGGACCTTGCAAAGTTA+TGG - Chr5:29311008-29311027 MsG0580026024.01.T01:intron 45.0%
! TAAAGTGGTGGGAGCAAGAA+AGG - Chr5:29311317-29311336 MsG0580026024.01.T01:CDS 45.0%
TGGCAATGAATGCACCTGGA+AGG + Chr5:29311262-29311281 None:intergenic 50.0%
! AGTGGTGGGAGCAAGAAAGG+AGG - Chr5:29311320-29311339 MsG0580026024.01.T01:CDS 55.0%
!! GGGAGTGTCTCAAACCTTCC+AGG - Chr5:29311245-29311264 MsG0580026024.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 29310767 29311377 29310767 ID=MsG0580026024.01;Name=MsG0580026024.01
Chr5 mRNA 29310767 29311377 29310767 ID=MsG0580026024.01.T01;Parent=MsG0580026024.01;Name=MsG0580026024.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|1|1|3|0|126
Chr5 exon 29311252 29311377 29311252 ID=MsG0580026024.01.T01:exon:33455;Parent=MsG0580026024.01.T01
Chr5 exon 29311040 29311162 29311040 ID=MsG0580026024.01.T01:exon:33454;Parent=MsG0580026024.01.T01
Chr5 exon 29310767 29310898 29310767 ID=MsG0580026024.01.T01:exon:33453;Parent=MsG0580026024.01.T01
Chr5 CDS 29311252 29311377 29311252 ID=MsG0580026024.01.T01:cds;Parent=MsG0580026024.01.T01
Chr5 CDS 29311040 29311162 29311040 ID=MsG0580026024.01.T01:cds;Parent=MsG0580026024.01.T01
Chr5 CDS 29310767 29310898 29310767 ID=MsG0580026024.01.T01:cds;Parent=MsG0580026024.01.T01
Gene Sequence

>MsG0580026024.01.T01

ATGAGTTCCAACTTGTATAATTTTGTGTATGTTGATAGATCAACAGAATCATCCCATGTCCTTACATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTTAAAGAAACTAATAAACTGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTTTGAAAGATTTTGCTGCAAAATACACTGATGTTGAGTTCATTAAGCTTGATGTTGATGAGTTATTGGGAGTGTCTCAAACCTTCCAGGTGCATTCATTGCCAACTTTTCTACTCATTAAGAAAGGAAAGGTTGTTGATAAAGTGGTGGGAGCAAGAAAGGAGGAACTACAAAGGATGATTGAAAAACAAAGAAATTAA

Protein sequence

>MsG0580026024.01.T01

MSSNLYNFVYVDRSTESSHVLTFHSTAKWNAHFEAFKETNKLMVIDFTAKWCGPCKVMDPVLKDFAAKYTDVEFIKLDVDELLGVSQTFQVHSLPTFLLIKKGKVVDKVVGARKEELQRMIEKQRN*