AlfalfaGEDB | MsG0580026024.01

M. sativa cultivar ZhongmuNo.1 / MsG0580026024.01

KEGG: K03671
GO: GO:0006662 GO:0015035 GO:0045454
PFAM: PF00085

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026024.01.T01	XP_003613544.1	97.619	126	3	0	1	126	1	126	5.97E-88	261

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026024.01.T01	Q38879	48.649	111	57	0	15	125	22	132	5.96E-41	135

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026024.01.T01	D8L5E0	97.619	126	3	0	1	126	1	126	2.85e-88	261

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048969.01	MsG0580026024.01	0.826665	2.395172e-54	2.260830e-51
MsG0080049130.01	MsG0580026024.01	0.805121	1.555362e-49	8.162095e-47
MsG0180000128.01	MsG0580026024.01	0.809408	1.920699e-50	1.126838e-47
MsG0180001523.01	MsG0580026024.01	0.800815	1.207114e-48	5.674563e-46
MsG0180003274.01	MsG0580026024.01	0.805980	1.027227e-49	5.510965e-47
MsG0180003336.01	MsG0580026024.01	0.827418	1.581598e-54	1.526135e-51
MsG0180003352.01	MsG0580026024.01	0.832478	9.239091e-56	1.033908e-52
MsG0180003451.01	MsG0580026024.01	0.820287	7.429008e-53	5.853537e-50
MsG0180003649.01	MsG0580026024.01	0.810494	1.120862e-50	6.766422e-48
MsG0180004937.01	MsG0580026024.01	0.858364	8.666393e-63	2.206322e-59

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PPI

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Gene1	Gene2	Type
MsG0580026024.01	MsG0280006562.01	PPI
MsG0580026024.01	MsG0280006562.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026024.01.T01	MTR_5g037890	97.619	126	3	0	1	126	1	126	7.23e-92	261
MsG0580026024.01.T01	MTR_5g037950	66.667	126	41	1	1	125	1	126	2.69e-52	161
MsG0580026024.01.T01	MTR_5g037930	66.406	128	41	1	1	126	1	128	3.74e-52	161
MsG0580026024.01.T01	MTR_7g009070	57.547	106	45	0	20	125	28	133	3.46e-44	141
MsG0580026024.01.T01	MTR_3g112410	47.748	111	58	0	14	124	2	112	2.13e-36	120
MsG0580026024.01.T01	MTR_1g023140	43.363	113	64	0	14	126	2	114	1.23e-34	116
MsG0580026024.01.T01	MTR_5g021180	45.045	111	61	0	14	124	4	114	1.90e-34	116
MsG0580026024.01.T01	MTR_8g107460	42.308	104	60	0	18	121	254	357	9.44e-25	96.7
MsG0580026024.01.T01	MTR_8g005320	34.286	105	69	0	17	121	25	129	2.54e-23	88.2
MsG0580026024.01.T01	MTR_7g073680	36.190	105	67	0	17	121	23	127	3.37e-23	88.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026024.01.T01	AT5G39950	48.649	111	57	0	15	125	22	132	6.07e-42	135
MsG0580026024.01.T01	AT1G69880	47.664	107	56	0	17	123	36	142	5.77e-35	118
MsG0580026024.01.T01	AT1G59730	41.406	128	72	1	1	125	1	128	6.26e-35	117
MsG0580026024.01.T01	AT3G51030	42.727	110	63	0	14	123	2	111	2.85e-33	112
MsG0580026024.01.T01	AT1G19730	46.429	112	59	1	14	124	2	113	1.38e-32	111
MsG0580026024.01.T01	AT1G45145	43.925	107	60	0	18	124	5	111	2.17e-31	108
MsG0580026024.01.T01	AT5G42980	44.037	109	61	0	18	126	5	113	7.94e-31	107
MsG0580026024.01.T01	AT3G17880	40.777	103	61	0	19	121	264	366	1.45e-22	91.3
MsG0580026024.01.T01	AT3G17880	40.777	103	61	0	19	121	271	373	1.46e-22	91.3
MsG0580026024.01.T01	AT3G56420	38.043	92	57	0	28	119	50	141	2.87e-21	83.6

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Find 24 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTTGTTGATAAAGTGG+TGG	0.610487	5:-29310817	MsG0580026024.01.T01:CDS
AAGGTTGTTGATAAAGTGGT+GGG	0.601867	5:-29310816	MsG0580026024.01.T01:CDS
AAGTTGGCAATGAATGCACC+TGG	0.579245	5:+29310859	None:intergenic
ACTGGATCCATAACTTTGCA+AGG	0.608925	5:+29311107	None:intergenic
AGGAAAGGTTGTTGATAAAG+TGG	0.493130	5:-29310820	MsG0580026024.01.T01:CDS
AGTGGTGGGAGCAAGAAAGG+AGG	0.703427	5:-29310802	MsG0580026024.01.T01:CDS
ATACACAAAATTATACAAGT+TGG	0.527315	5:+29311348	None:intergenic
ATGAGCATTCCATTTAGCAG+TGG	0.609708	5:+29311282	None:intergenic
CAAGAAAGGAGGAACTACAA+AGG	0.696570	5:-29310791	MsG0580026024.01.T01:CDS
CATTCCATTCCACTGCTAAA+TGG	0.238150	5:-29311291	MsG0580026024.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTACTCATTAAGAAAGGAA+AGG	-	Chr5:29311287-29311306	MsG0580026024.01.T01:CDS	30.0%
CCACAATATAATTGGCAACA+GGG	-	Chr5:29311225-29311244	MsG0580026024.01.T01:intron	35.0%
CCTGTTGCCAATTATATTGT+GGG	+	Chr5:29311227-29311246	None:intergenic	35.0%
GCAGCAAAATCTTTCAAAAC+TGG	+	Chr5:29311036-29311055	None:intergenic	35.0%
ACAAATCAGTTCTCAAGTGC+TGG	+	Chr5:29311200-29311219	None:intergenic	40.0%
ACTGGATCCATAACTTTGCA+AGG	+	Chr5:29311018-29311037	None:intergenic	40.0%
ATGAGCATTCCATTTAGCAG+TGG	+	Chr5:29310843-29310862	None:intergenic	40.0%
CAAGAAAGGAGGAACTACAA+AGG	-	Chr5:29311331-29311350	MsG0580026024.01.T01:CDS	40.0%
CATTCCATTCCACTGCTAAA+TGG	-	Chr5:29310831-29310850	MsG0580026024.01.T01:CDS	40.0%
CATTCCATTTAGCAGTGGAA+TGG	+	Chr5:29310838-29310857	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	29310767	29311377	29310767	ID=MsG0580026024.01;Name=MsG0580026024.01
Chr5	mRNA	29310767	29311377	29310767	ID=MsG0580026024.01.T01;Parent=MsG0580026024.01;Name=MsG0580026024.01.T01;_AED=0.45;_eAED=0.45;_QI=0\|0\|0\|1\|1\|1\|3\|0\|126
Chr5	exon	29311252	29311377	29311252	ID=MsG0580026024.01.T01:exon:33455;Parent=MsG0580026024.01.T01
Chr5	exon	29311040	29311162	29311040	ID=MsG0580026024.01.T01:exon:33454;Parent=MsG0580026024.01.T01
Chr5	exon	29310767	29310898	29310767	ID=MsG0580026024.01.T01:exon:33453;Parent=MsG0580026024.01.T01
Chr5	CDS	29311252	29311377	29311252	ID=MsG0580026024.01.T01:cds;Parent=MsG0580026024.01.T01
Chr5	CDS	29311040	29311162	29311040	ID=MsG0580026024.01.T01:cds;Parent=MsG0580026024.01.T01
Chr5	CDS	29310767	29310898	29310767	ID=MsG0580026024.01.T01:cds;Parent=MsG0580026024.01.T01

Gene Sequence

>MsG0580026024.01.T01

ATGAGTTCCAACTTGTATAATTTTGTGTATGTTGATAGATCAACAGAATCATCCCATGTCCTTACATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTTAAAGAAACTAATAAACTGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTTTGAAAGATTTTGCTGCAAAATACACTGATGTTGAGTTCATTAAGCTTGATGTTGATGAGTTATTGGGAGTGTCTCAAACCTTCCAGGTGCATTCATTGCCAACTTTTCTACTCATTAAGAAAGGAAAGGTTGTTGATAAAGTGGTGGGAGCAAGAAAGGAGGAACTACAAAGGATGATTGAAAAACAAAGAAATTAA

Protein sequence

>MsG0580026024.01.T01

MSSNLYNFVYVDRSTESSHVLTFHSTAKWNAHFEAFKETNKLMVIDFTAKWCGPCKVMDPVLKDFAAKYTDVEFIKLDVDELLGVSQTFQVHSLPTFLLIKKGKVVDKVVGARKEELQRMIEKQRN*