Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015813.01.T01 | XP_003601280.1 | 97.368 | 304 | 8 | 0 | 1 | 304 | 1 | 304 | 0 | 597 |
| MsG0380015813.01.T02 | XP_003601280.1 | 97.5 | 280 | 7 | 0 | 1 | 280 | 25 | 304 | 0 | 545 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015813.01.T01 | O64761 | 78.859 | 298 | 62 | 1 | 8 | 304 | 6 | 303 | 1.02E-139 | 399 |
| MsG0380015813.01.T02 | O64761 | 79.715 | 281 | 56 | 1 | 1 | 280 | 23 | 303 | 1.06E-130 | 375 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015813.01.T01 | G7J5H5 | 97.368 | 304 | 8 | 0 | 1 | 304 | 1 | 304 | 0.0 | 597 |
| MsG0380015813.01.T02 | G7J5H5 | 97.500 | 280 | 7 | 0 | 1 | 280 | 25 | 304 | 0.0 | 545 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004293.01 | MsG0380015813.01 | 0.801910 | 7.205422e-49 | 3.482444e-46 |
| MsG0380015813.01 | MsG0480023384.01 | -0.806970 | 6.351954e-50 | 3.495124e-47 |
| MsG0380015813.01 | MsG0580024619.01 | -0.805111 | 1.562714e-49 | 8.198483e-47 |
| MsG0380015813.01 | MsG0880045480.01 | 0.808364 | 3.211026e-50 | 1.832559e-47 |
| MsG0380015813.01 | MsG0880046867.01 | -0.810294 | 1.238135e-50 | 7.434710e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015813.01.T01 | MTR_3g077990 | 97.368 | 304 | 8 | 0 | 1 | 304 | 1 | 304 | 0.0 | 597 |
| MsG0380015813.01.T01 | MTR_3g464840 | 84.483 | 58 | 9 | 0 | 198 | 255 | 1729 | 1786 | 5.81e-24 | 102 |
| MsG0380015813.01.T02 | MTR_3g077990 | 97.500 | 280 | 7 | 0 | 1 | 280 | 25 | 304 | 0.0 | 545 |
| MsG0380015813.01.T02 | MTR_3g464840 | 84.483 | 58 | 9 | 0 | 174 | 231 | 1729 | 1786 | 1.67e-23 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015813.01.T01 | AT2G34980 | 78.859 | 298 | 62 | 1 | 8 | 304 | 6 | 303 | 1.04e-140 | 399 |
| MsG0380015813.01.T02 | AT2G34980 | 79.715 | 281 | 56 | 1 | 1 | 280 | 23 | 303 | 1.08e-131 | 375 |
Find 61 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACTTGGTTTGCCCTTAT+TGG | 0.113834 | 3:+75428718 | MsG0380015813.01.T01:CDS |
| CCATTAGTTCTGATTCTATT+TGG | 0.139282 | 3:+75428337 | MsG0380015813.01.T01:CDS |
| CATAACTGGTTTATATGTTT+TGG | 0.181603 | 3:+75428287 | MsG0380015813.01.T01:CDS |
| GATGTAAGTCTTCTCGTTTC+AGG | 0.212723 | 3:+75428186 | MsG0380015813.01.T01:CDS |
| TTTGCCCTTATTGGCTTATA+AGG | 0.252639 | 3:+75428727 | MsG0380015813.01.T01:CDS |
| TTATTGGCTTATAAGGATTC+AGG | 0.266337 | 3:+75428734 | MsG0380015813.01.T01:CDS |
| TGAATCCTTATAAGCCAATA+AGG | 0.269795 | 3:-75428732 | None:intergenic |
| AAAGCATTTCTTGAGTAAAA+AGG | 0.276516 | 3:-75428221 | None:intergenic |
| TGTGCTGCTACTTAGTTTGT+TGG | 0.298882 | 3:+75428689 | MsG0380015813.01.T01:CDS |
| TTGCTCTTGTTGTCTTGGTC+TGG | 0.321036 | 3:+75428115 | MsG0380015813.01.T01:CDS |
| CATCATGTTATTCTCGTTGC+AGG | 0.326129 | 3:+75428539 | MsG0380015813.01.T01:CDS |
| CATTGTTGCTCTTGTTGTCT+TGG | 0.328685 | 3:+75428110 | MsG0380015813.01.T01:CDS |
| CATTAGTTCTGATTCTATTT+GGG | 0.331805 | 3:+75428338 | MsG0380015813.01.T01:CDS |
| TATTGTAATGGATGTCAAAA+TGG | 0.373197 | 3:+75427900 | None:intergenic |
| ACCGATGAAACCTTTCTTGA+AGG | 0.377730 | 3:+75428006 | MsG0380015813.01.T01:CDS |
| TGAATGCTAGTGTTGTAAAA+AGG | 0.384855 | 3:+75428037 | MsG0380015813.01.T01:CDS |
| CATGACCATTCCTTCAAGAA+AGG | 0.407072 | 3:-75428016 | None:intergenic |
| ATGTAACCAGTGGAGCAAAA+AGG | 0.410867 | 3:-75428566 | None:intergenic |
| TTTGTTGGTCTTTGTCAACT+TGG | 0.414907 | 3:+75428704 | MsG0380015813.01.T01:CDS |
| TAATGGACCTCGTAAGAGTT+TGG | 0.421250 | 3:-75428320 | None:intergenic |
| GCAAACACATGTAGTCTTGA+TGG | 0.423869 | 3:-75428516 | None:intergenic |
| AACTAAGTAGCAGCACAAAT+AGG | 0.429506 | 3:-75428683 | None:intergenic |
| CCAAATAGAATCAGAACTAA+TGG | 0.429636 | 3:-75428337 | None:intergenic |
| CTGAATAATCATGCAGGAAA+AGG | 0.430468 | 3:-75428389 | None:intergenic |
| GCAGGGTTCTTCAAAGCCCC+TGG | 0.463363 | 3:-75428429 | None:intergenic |
| AATGCATAGATATTCAGAAA+TGG | 0.466377 | 3:-75428091 | None:intergenic |
| AGCATTTACAGATATACAAC+TGG | 0.476097 | 3:-75428457 | None:intergenic |
| CAGTGTGTACACAAAACTTA+AGG | 0.477379 | 3:-75428652 | None:intergenic |
| CTTCTCCGCCTCGAGCCAAA+TGG | 0.488538 | 3:+75427935 | MsG0380015813.01.T01:CDS |
| GAATCCACTGTCAAAGCCCC+AGG | 0.489514 | 3:+75428411 | MsG0380015813.01.T01:CDS |
| GAATATAAGTTTGAGATCAA+TGG | 0.499009 | 3:+75428756 | MsG0380015813.01.T01:CDS |
| GCCTATGGTGGTATGCAACC+TGG | 0.501903 | 3:+75427967 | MsG0380015813.01.T01:CDS |
| GAACATGCTAAAGGTGATGC+TGG | 0.502172 | 3:+75428059 | MsG0380015813.01.T01:CDS |
| GAATCCTTATAAGCCAATAA+GGG | 0.505222 | 3:-75428731 | None:intergenic |
| GATATACAACTGGTTAATGC+AGG | 0.505761 | 3:-75428447 | None:intergenic |
| AGCCCCTGGGGCTTTGACAG+TGG | 0.507837 | 3:-75428415 | None:intergenic |
| CAGGGTTCTTCAAAGCCCCT+GGG | 0.509672 | 3:-75428428 | None:intergenic |
| ACAAAGCTTAGCCTCGTCCC+AGG | 0.517211 | 3:-75428781 | None:intergenic |
| TGAAACCTTTCTTGAAGGAA+TGG | 0.519707 | 3:+75428011 | MsG0380015813.01.T01:CDS |
| TCCTTCAAGAAAGGTTTCAT+CGG | 0.524359 | 3:-75428007 | None:intergenic |
| CTCGTAAGAGTTTGGTAGAT+AGG | 0.533723 | 3:-75428312 | None:intergenic |
| GTATGATTGTCATCATATCC+AGG | 0.540147 | 3:-75427985 | None:intergenic |
| AGATATTGAGGGCATAATGA+AGG | 0.541095 | 3:-75428257 | None:intergenic |
| AAATGCTTCTGTAGTCGCCT+CGG | 0.545200 | 3:+75428473 | MsG0380015813.01.T01:CDS |
| AAATGGAGAAAAGTAGCCTA+TGG | 0.557172 | 3:+75427952 | MsG0380015813.01.T01:CDS |
| TGGATTCTGAATAATCATGC+AGG | 0.568914 | 3:-75428395 | None:intergenic |
| AAAGCATATGTGTAAGCAGA+AGG | 0.584702 | 3:-75428607 | None:intergenic |
| GATCAATGGACCCTGGGACG+AGG | 0.585309 | 3:+75428770 | MsG0380015813.01.T01:CDS |
| TTAATACAGTATGTAACCAG+TGG | 0.586025 | 3:-75428576 | None:intergenic |
| GTTTGAGATCAATGGACCCT+GGG | 0.600084 | 3:+75428764 | MsG0380015813.01.T01:CDS |
| AGTTTGAGATCAATGGACCC+TGG | 0.600417 | 3:+75428763 | MsG0380015813.01.T01:CDS |
| TCCAGGTTGCATACCACCAT+AGG | 0.605048 | 3:-75427968 | None:intergenic |
| AATCCACTGTCAAAGCCCCA+GGG | 0.616730 | 3:+75428412 | MsG0380015813.01.T01:CDS |
| TTTCTCCATTTGGCTCGAGG+CGG | 0.617404 | 3:-75427940 | None:intergenic |
| CAAAGCTTAGCCTCGTCCCA+GGG | 0.620911 | 3:-75428780 | None:intergenic |
| CTGCAACGAGAATAACATGA+TGG | 0.622923 | 3:-75428538 | None:intergenic |
| CTATCTACCAAACTCTTACG+AGG | 0.647536 | 3:+75428313 | MsG0380015813.01.T01:CDS |
| TGGAGAAAAGTAGCCTATGG+TGG | 0.650401 | 3:+75427955 | MsG0380015813.01.T01:CDS |
| ATATACAACTGGTTAATGCA+GGG | 0.660347 | 3:-75428446 | None:intergenic |
| AGGGTTCTTCAAAGCCCCTG+GGG | 0.663398 | 3:-75428427 | None:intergenic |
| ATCCACTGTCAAAGCCCCAG+GGG | 0.705854 | 3:+75428413 | MsG0380015813.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGAAAAATGAGATATTGA+GGG | - | Chr3:75428271-75428290 | None:intergenic | 20.0% |
| !! | TTATGAAAAATGAGATATTG+AGG | - | Chr3:75428272-75428291 | None:intergenic | 20.0% |
| !!! | AATATCTCATTTTTCATAAC+TGG | + | Chr3:75428273-75428292 | MsG0380015813.01.T01:CDS | 20.0% |
| ! | AATGCATAGATATTCAGAAA+TGG | - | Chr3:75428094-75428113 | None:intergenic | 25.0% |
| !! | AAAGCATTTCTTGAGTAAAA+AGG | - | Chr3:75428224-75428243 | None:intergenic | 25.0% |
| !! | GAATATAAGTTTGAGATCAA+TGG | + | Chr3:75428756-75428775 | MsG0380015813.01.T01:CDS | 25.0% |
| !!! | ATGCTTTTCTATTAGTTTGA+TGG | + | Chr3:75428623-75428642 | MsG0380015813.01.T01:CDS | 25.0% |
| !!! | CATAACTGGTTTATATGTTT+TGG | + | Chr3:75428287-75428306 | MsG0380015813.01.T01:CDS | 25.0% |
| !!! | CATTAGTTCTGATTCTATTT+GGG | + | Chr3:75428338-75428357 | MsG0380015813.01.T01:CDS | 25.0% |
| AGCATTTACAGATATACAAC+TGG | - | Chr3:75428460-75428479 | None:intergenic | 30.0% | |
| ATATACAACTGGTTAATGCA+GGG | - | Chr3:75428449-75428468 | None:intergenic | 30.0% | |
| CCAAATAGAATCAGAACTAA+TGG | - | Chr3:75428340-75428359 | None:intergenic | 30.0% | |
| GAATCCTTATAAGCCAATAA+GGG | - | Chr3:75428734-75428753 | None:intergenic | 30.0% | |
| TGAATCCTTATAAGCCAATA+AGG | - | Chr3:75428735-75428754 | None:intergenic | 30.0% | |
| TGAATGCTAGTGTTGTAAAA+AGG | + | Chr3:75428037-75428056 | MsG0380015813.01.T01:CDS | 30.0% | |
| TGTAAAAAGGAACATGCTAA+AGG | + | Chr3:75428050-75428069 | MsG0380015813.01.T01:CDS | 30.0% | |
| TTAATACAGTATGTAACCAG+TGG | - | Chr3:75428579-75428598 | None:intergenic | 30.0% | |
| !! | CCATTAGTTCTGATTCTATT+TGG | + | Chr3:75428337-75428356 | MsG0380015813.01.T01:CDS | 30.0% |
| !! | TTATTGGCTTATAAGGATTC+AGG | + | Chr3:75428734-75428753 | MsG0380015813.01.T01:CDS | 30.0% |
| AAAGCATATGTGTAAGCAGA+AGG | - | Chr3:75428610-75428629 | None:intergenic | 35.0% | |
| AAATGGAGAAAAGTAGCCTA+TGG | + | Chr3:75427952-75427971 | MsG0380015813.01.T01:CDS | 35.0% | |
| AACTAAGTAGCAGCACAAAT+AGG | - | Chr3:75428686-75428705 | None:intergenic | 35.0% | |
| CAGTGTGTACACAAAACTTA+AGG | - | Chr3:75428655-75428674 | None:intergenic | 35.0% | |
| CTGAATAATCATGCAGGAAA+AGG | - | Chr3:75428392-75428411 | None:intergenic | 35.0% | |
| GATATACAACTGGTTAATGC+AGG | - | Chr3:75428450-75428469 | None:intergenic | 35.0% | |
| GTATGATTGTCATCATATCC+AGG | - | Chr3:75427988-75428007 | None:intergenic | 35.0% | |
| TCCTTCAAGAAAGGTTTCAT+CGG | - | Chr3:75428010-75428029 | None:intergenic | 35.0% | |
| TGAAACCTTTCTTGAAGGAA+TGG | + | Chr3:75428011-75428030 | MsG0380015813.01.T01:CDS | 35.0% | |
| TGGATTCTGAATAATCATGC+AGG | - | Chr3:75428398-75428417 | None:intergenic | 35.0% | |
| ! | AGATATTGAGGGCATAATGA+AGG | - | Chr3:75428260-75428279 | None:intergenic | 35.0% |
| !! | TTTGCCCTTATTGGCTTATA+AGG | + | Chr3:75428727-75428746 | MsG0380015813.01.T01:CDS | 35.0% |
| !! | TTTGTTGGTCTTTGTCAACT+TGG | + | Chr3:75428704-75428723 | MsG0380015813.01.T01:CDS | 35.0% |
| !!! | ATAGGCTACTTTTCTCCATT+TGG | - | Chr3:75427953-75427972 | None:intergenic | 35.0% |
| ACCGATGAAACCTTTCTTGA+AGG | + | Chr3:75428006-75428025 | MsG0380015813.01.T01:CDS | 40.0% | |
| ACGAGAAGCAATAAAAACCG+AGG | - | Chr3:75428493-75428512 | None:intergenic | 40.0% | |
| ATGTAACCAGTGGAGCAAAA+AGG | - | Chr3:75428569-75428588 | None:intergenic | 40.0% | |
| CATCATGTTATTCTCGTTGC+AGG | + | Chr3:75428539-75428558 | MsG0380015813.01.T01:CDS | 40.0% | |
| CATGACCATTCCTTCAAGAA+AGG | - | Chr3:75428019-75428038 | None:intergenic | 40.0% | |
| CTATCTACCAAACTCTTACG+AGG | + | Chr3:75428313-75428332 | MsG0380015813.01.T01:CDS | 40.0% | |
| CTCGTAAGAGTTTGGTAGAT+AGG | - | Chr3:75428315-75428334 | None:intergenic | 40.0% | |
| CTGCAACGAGAATAACATGA+TGG | - | Chr3:75428541-75428560 | None:intergenic | 40.0% | |
| GATGTAAGTCTTCTCGTTTC+AGG | + | Chr3:75428186-75428205 | MsG0380015813.01.T01:CDS | 40.0% | |
| ! | CATTGTTGCTCTTGTTGTCT+TGG | + | Chr3:75428110-75428129 | MsG0380015813.01.T01:CDS | 40.0% |
| ! | GCAAACACATGTAGTCTTGA+TGG | - | Chr3:75428519-75428538 | None:intergenic | 40.0% |
| ! | TAATGGACCTCGTAAGAGTT+TGG | - | Chr3:75428323-75428342 | None:intergenic | 40.0% |
| !! | TCAACTTGGTTTGCCCTTAT+TGG | + | Chr3:75428718-75428737 | MsG0380015813.01.T01:CDS | 40.0% |
| !! | TGTGCTGCTACTTAGTTTGT+TGG | + | Chr3:75428689-75428708 | MsG0380015813.01.T01:CDS | 40.0% |
| AAATGCTTCTGTAGTCGCCT+CGG | + | Chr3:75428473-75428492 | MsG0380015813.01.T01:CDS | 45.0% | |
| GAACATGCTAAAGGTGATGC+TGG | + | Chr3:75428059-75428078 | MsG0380015813.01.T01:CDS | 45.0% | |
| TGGAGAAAAGTAGCCTATGG+TGG | + | Chr3:75427955-75427974 | MsG0380015813.01.T01:CDS | 45.0% | |
| ! | AGTTTGAGATCAATGGACCC+TGG | + | Chr3:75428763-75428782 | MsG0380015813.01.T01:CDS | 45.0% |
| ! | GGTTTTCCTTTTTGCTCCAC+TGG | + | Chr3:75428560-75428579 | MsG0380015813.01.T01:CDS | 45.0% |
| ! | GTTTGAGATCAATGGACCCT+GGG | + | Chr3:75428764-75428783 | MsG0380015813.01.T01:CDS | 45.0% |
| ! | TTGCTCTTGTTGTCTTGGTC+TGG | + | Chr3:75428115-75428134 | MsG0380015813.01.T01:CDS | 45.0% |
| !! | ACTTTTCTCCATTTGGCTCG+AGG | - | Chr3:75427946-75427965 | None:intergenic | 45.0% |
| AATCCACTGTCAAAGCCCCA+GGG | + | Chr3:75428412-75428431 | MsG0380015813.01.T01:CDS | 50.0% | |
| TCCAGGTTGCATACCACCAT+AGG | - | Chr3:75427971-75427990 | None:intergenic | 50.0% | |
| ! | TTTCTCCATTTGGCTCGAGG+CGG | - | Chr3:75427943-75427962 | None:intergenic | 50.0% |
| ACAAAGCTTAGCCTCGTCCC+AGG | - | Chr3:75428784-75428803 | None:intergenic | 55.0% | |
| AGGGTTCTTCAAAGCCCCTG+GGG | - | Chr3:75428430-75428449 | None:intergenic | 55.0% | |
| ATCCACTGTCAAAGCCCCAG+GGG | + | Chr3:75428413-75428432 | MsG0380015813.01.T01:CDS | 55.0% | |
| CAAAGCTTAGCCTCGTCCCA+GGG | - | Chr3:75428783-75428802 | None:intergenic | 55.0% | |
| CAGGGTTCTTCAAAGCCCCT+GGG | - | Chr3:75428431-75428450 | None:intergenic | 55.0% | |
| GAATCCACTGTCAAAGCCCC+AGG | + | Chr3:75428411-75428430 | MsG0380015813.01.T01:CDS | 55.0% | |
| GCCTATGGTGGTATGCAACC+TGG | + | Chr3:75427967-75427986 | MsG0380015813.01.T01:CDS | 55.0% | |
| CTTCTCCGCCTCGAGCCAAA+TGG | + | Chr3:75427935-75427954 | MsG0380015813.01.T01:CDS | 60.0% | |
| GATCAATGGACCCTGGGACG+AGG | + | Chr3:75428770-75428789 | MsG0380015813.01.T01:CDS | 60.0% | |
| GCAGGGTTCTTCAAAGCCCC+TGG | - | Chr3:75428432-75428451 | None:intergenic | 60.0% | |
| ! | AGCCCCTGGGGCTTTGACAG+TGG | - | Chr3:75428418-75428437 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 75427907 | 75428821 | 75427907 | ID=MsG0380015813.01;Name=MsG0380015813.01 |
| Chr3 | mRNA | 75427907 | 75428821 | 75427907 | ID=MsG0380015813.01.T01;Parent=MsG0380015813.01;Name=MsG0380015813.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|304 |
| Chr3 | exon | 75427907 | 75428821 | 75427907 | ID=MsG0380015813.01.T01:exon:14466;Parent=MsG0380015813.01.T01 |
| Chr3 | CDS | 75427907 | 75428821 | 75427907 | ID=MsG0380015813.01.T01:cds;Parent=MsG0380015813.01.T01 |
| Chr3 | mRNA | 75427979 | 75428821 | 75427979 | ID=MsG0380015813.01.T02;Parent=MsG0380015813.01;Name=MsG0380015813.01.T02;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|280 |
| Chr3 | exon | 75427979 | 75428821 | 75427979 | ID=MsG0380015813.01.T02:exon:14467;Parent=MsG0380015813.01.T02 |
| Chr3 | CDS | 75427979 | 75428821 | 75427979 | ID=MsG0380015813.01.T02:cds;Parent=MsG0380015813.01.T02 |
| Gene Sequence |
| Protein sequence |
