AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046867.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046867.01.T01 MTR_4g116200 95.753 259 8 2 1 259 1 256 0.0 514
MsG0880046867.01.T01 MTR_2g450720 52.830 265 93 9 1 259 1 239 1.95e-82 247
MsG0880046867.01.T01 MTR_4g009760 48.667 150 68 4 1 142 1 149 8.13e-38 132
MsG0880046867.01.T01 MTR_2g104230 46.250 160 69 6 1 146 1 157 2.96e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046867.01.T01 AT2G12462 33.571 280 134 13 1 259 1 249 1.32e-33 122
MsG0880046867.01.T01 AT1G15760 39.157 166 87 3 3 162 5 162 4.82e-31 114
MsG0880046867.01.T01 AT1G80520 51.923 104 43 2 1 98 1 103 3.56e-29 109

Find 48 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCAATGCTTTATTATTT+TGG 0.098715 8:-79249082 None:intergenic
TCACCTTTGGGTTTATTATA+AGG 0.198904 8:-79249314 None:intergenic
TCAATAGCCCTACTTCTTAA+TGG 0.233145 8:-79249167 None:intergenic
CCATCTAATGGTCCTGAATA+TGG 0.284057 8:-79249035 None:intergenic
ATCAAAATCAAAATCACCTT+TGG 0.295369 8:-79249327 None:intergenic
AATAAAGCATTGAAGTTATC+AGG 0.300318 8:+79249089 MsG0880046867.01.T01:CDS
CATTCACTCATATATGATTA+TGG 0.310367 8:+79248660 MsG0880046867.01.T01:CDS
ACCAAGAGTCCAAGAGTATC+TGG 0.350418 8:+79249200 MsG0880046867.01.T01:CDS
GAGAATCTCTAGTCTGTGTT+TGG 0.350721 8:-79248769 None:intergenic
TTCATCAATCTTCCATCAAT+TGG 0.355382 8:-79249113 None:intergenic
ACAGAATTCCTTGTAATAGT+TGG 0.363635 8:-79249260 None:intergenic
AGCAGAACAATGCCATATTC+AGG 0.374090 8:+79249023 MsG0880046867.01.T01:CDS
TTCCAACTGAAGCTCATTGC+GGG 0.374523 8:-79248694 None:intergenic
TCCAAGAGTATCTGGACCTT+TGG 0.386924 8:+79249208 MsG0880046867.01.T01:CDS
TCAAAATCAAAATCACCTTT+GGG 0.389288 8:-79249326 None:intergenic
TCATGCATTCTTCCATCTAA+TGG 0.401833 8:-79249047 None:intergenic
AAGTTATCAGGACCAATTGA+TGG 0.410093 8:+79249101 MsG0880046867.01.T01:CDS
CCATATTCAGGACCATTAGA+TGG 0.411197 8:+79249035 MsG0880046867.01.T01:CDS
TCCAGATACTCTTGGACTCT+TGG 0.414833 8:-79249201 None:intergenic
ATAGAAGTCCATTAAGAAGT+AGG 0.426654 8:+79249159 MsG0880046867.01.T01:CDS
ATTGTTCTGCTTCTATACAT+AGG 0.436227 8:-79249011 None:intergenic
AAGAATGCATGAGCAGAAAA+TGG 0.442330 8:+79249058 MsG0880046867.01.T01:CDS
TGGTTCTACACGAACCGAAT+TGG 0.445561 8:+79248934 MsG0880046867.01.T01:CDS
TGTTGAAACTGATAGCCCTA+TGG 0.446699 8:+79249280 MsG0880046867.01.T01:CDS
TTAAGAAGTAGGGCTATTGA+TGG 0.487712 8:+79249170 MsG0880046867.01.T01:CDS
TCGTGTAGAACCACAGGTAA+AGG 0.487940 8:-79248924 None:intergenic
CATAATCATATATGAGTGAA+TGG 0.492231 8:-79248659 None:intergenic
CCATGCTTTGTAGAAACTCA+TGG 0.497469 8:-79248734 None:intergenic
GTTGAAACTGATAGCCCTAT+GGG 0.514418 8:+79249281 MsG0880046867.01.T01:CDS
TCCAAAGGTCCAGATACTCT+TGG 0.514906 8:-79249209 None:intergenic
CCATGAGTTTCTACAAAGCA+TGG 0.527981 8:+79248734 MsG0880046867.01.T01:CDS
TAGAAGTCCATTAAGAAGTA+GGG 0.530916 8:+79249160 MsG0880046867.01.T01:CDS
TTCCCGCAATGAGCTTCAGT+TGG 0.551524 8:+79248692 MsG0880046867.01.T01:CDS
TTCGGTTCGTGTAGAACCAC+AGG 0.553276 8:-79248930 None:intergenic
TAAGGACTACTATAACCCAT+AGG 0.553316 8:-79249296 None:intergenic
TTGATGATGAGCATTCACTT+TGG 0.562009 8:+79249345 MsG0880046867.01.T01:CDS
ACGAACCGAATTGGTACCAC+GGG 0.566859 8:+79248943 MsG0880046867.01.T01:CDS
CATGAGTTTCTACAAAGCAT+GGG 0.601787 8:+79248735 MsG0880046867.01.T01:CDS
CTTCCAACTGAAGCTCATTG+CGG 0.606732 8:-79248695 None:intergenic
ATTGATGAATGAGAGAATGA+TGG 0.606802 8:+79249127 MsG0880046867.01.T01:CDS
AAGGACTACTATAACCCATA+GGG 0.607941 8:-79249295 None:intergenic
TTAAGATCTTGAAAGAGTGT+AGG 0.613928 8:-79249368 None:intergenic
TAACCATGAAAACCAGTCCA+TGG 0.615491 8:-79248619 None:intergenic
AGTCCTTATAATAAACCCAA+AGG 0.617768 8:+79249311 MsG0880046867.01.T01:CDS
ATGGAGAGGAGCATTGGTCA+AGG 0.622754 8:+79248971 MsG0880046867.01.T01:CDS
ATAGAAGTCCAACTATTACA+AGG 0.630633 8:+79249252 MsG0880046867.01.T01:CDS
CACGAACCGAATTGGTACCA+CGG 0.649002 8:+79248942 MsG0880046867.01.T01:CDS
GCTCATTGCGGGAAAAGACG+AGG 0.654195 8:-79248683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTCAATGCTTTATTATTT+TGG - Chr8:79249085-79249104 None:intergenic 20.0%
!!! TTTTTTTAGACACTTTTTGA+TGG - Chr8:79248856-79248875 None:intergenic 20.0%
! ATCAAAATCAAAATCACCTT+TGG - Chr8:79249330-79249349 None:intergenic 25.0%
! CATAATCATATATGAGTGAA+TGG - Chr8:79248662-79248681 None:intergenic 25.0%
! CATTCACTCATATATGATTA+TGG + Chr8:79248660-79248679 MsG0880046867.01.T01:CDS 25.0%
! TCAAAATCAAAATCACCTTT+GGG - Chr8:79249329-79249348 None:intergenic 25.0%
!!! AATAAAGCATTGAAGTTATC+AGG + Chr8:79249089-79249108 MsG0880046867.01.T01:CDS 25.0%
ACAGAATTCCTTGTAATAGT+TGG - Chr8:79249263-79249282 None:intergenic 30.0%
AGTCCTTATAATAAACCCAA+AGG + Chr8:79249311-79249330 MsG0880046867.01.T01:CDS 30.0%
ATAGAAGTCCAACTATTACA+AGG + Chr8:79249252-79249271 MsG0880046867.01.T01:CDS 30.0%
ATAGAAGTCCATTAAGAAGT+AGG + Chr8:79249159-79249178 MsG0880046867.01.T01:CDS 30.0%
ATTGATGAATGAGAGAATGA+TGG + Chr8:79249127-79249146 MsG0880046867.01.T01:CDS 30.0%
ATTGTTCTGCTTCTATACAT+AGG - Chr8:79249014-79249033 None:intergenic 30.0%
TAGAAGTCCATTAAGAAGTA+GGG + Chr8:79249160-79249179 MsG0880046867.01.T01:CDS 30.0%
TTCATCAATCTTCCATCAAT+TGG - Chr8:79249116-79249135 None:intergenic 30.0%
! AACATGATTTTTGCATCCAA+AGG - Chr8:79249227-79249246 None:intergenic 30.0%
! TCACCTTTGGGTTTATTATA+AGG - Chr8:79249317-79249336 None:intergenic 30.0%
! TTAAGATCTTGAAAGAGTGT+AGG - Chr8:79249371-79249390 None:intergenic 30.0%
AAGAATGCATGAGCAGAAAA+TGG + Chr8:79249058-79249077 MsG0880046867.01.T01:CDS 35.0%
AAGGACTACTATAACCCATA+GGG - Chr8:79249298-79249317 None:intergenic 35.0%
AAGTTATCAGGACCAATTGA+TGG + Chr8:79249101-79249120 MsG0880046867.01.T01:CDS 35.0%
CATGAGTTTCTACAAAGCAT+GGG + Chr8:79248735-79248754 MsG0880046867.01.T01:CDS 35.0%
TAAGGACTACTATAACCCAT+AGG - Chr8:79249299-79249318 None:intergenic 35.0%
TCAATAGCCCTACTTCTTAA+TGG - Chr8:79249170-79249189 None:intergenic 35.0%
TCATGCATTCTTCCATCTAA+TGG - Chr8:79249050-79249069 None:intergenic 35.0%
TTAAGAAGTAGGGCTATTGA+TGG + Chr8:79249170-79249189 MsG0880046867.01.T01:CDS 35.0%
TTACGACCAAAAAACCTTTC+TGG + Chr8:79248828-79248847 MsG0880046867.01.T01:CDS 35.0%
!! ACTTTTTGATGGTACCAGAA+AGG - Chr8:79248845-79248864 None:intergenic 35.0%
!! TTGATGATGAGCATTCACTT+TGG + Chr8:79249345-79249364 MsG0880046867.01.T01:CDS 35.0%
!!! ATGGTACCAGAAAGGTTTTT+TGG - Chr8:79248837-79248856 None:intergenic 35.0%
!!! GAAAGGTTTTTTGGTCGTAA+TGG - Chr8:79248828-79248847 None:intergenic 35.0%
AGCAGAACAATGCCATATTC+AGG + Chr8:79249023-79249042 MsG0880046867.01.T01:CDS 40.0%
CCATATTCAGGACCATTAGA+TGG + Chr8:79249035-79249054 MsG0880046867.01.T01:CDS 40.0%
CCATCTAATGGTCCTGAATA+TGG - Chr8:79249038-79249057 None:intergenic 40.0%
CCATGAGTTTCTACAAAGCA+TGG + Chr8:79248734-79248753 MsG0880046867.01.T01:CDS 40.0%
CCATGCTTTGTAGAAACTCA+TGG - Chr8:79248737-79248756 None:intergenic 40.0%
GAAAAACATGCCTTTACCTG+TGG + Chr8:79248914-79248933 MsG0880046867.01.T01:CDS 40.0%
GAGAATCTCTAGTCTGTGTT+TGG - Chr8:79248772-79248791 None:intergenic 40.0%
GTTGAAACTGATAGCCCTAT+GGG + Chr8:79249281-79249300 MsG0880046867.01.T01:CDS 40.0%
TGTTGAAACTGATAGCCCTA+TGG + Chr8:79249280-79249299 MsG0880046867.01.T01:CDS 40.0%
ATTGGTACCACGGGCAAAAA+TGG + Chr8:79248952-79248971 MsG0880046867.01.T01:CDS 45.0%
CTTCCAACTGAAGCTCATTG+CGG - Chr8:79248698-79248717 None:intergenic 45.0%
TCCAAAGGTCCAGATACTCT+TGG - Chr8:79249212-79249231 None:intergenic 45.0%
TCCAGATACTCTTGGACTCT+TGG - Chr8:79249204-79249223 None:intergenic 45.0%
TCGTGTAGAACCACAGGTAA+AGG - Chr8:79248927-79248946 None:intergenic 45.0%
TTCCAACTGAAGCTCATTGC+GGG - Chr8:79248697-79248716 None:intergenic 45.0%
! GCAAAAATGGAGAGGAGCAT+TGG + Chr8:79248965-79248984 MsG0880046867.01.T01:CDS 45.0%
! TGGTTCTACACGAACCGAAT+TGG + Chr8:79248934-79248953 MsG0880046867.01.T01:CDS 45.0%
! TTTTGCCCGTGGTACCAATT+CGG - Chr8:79248951-79248970 None:intergenic 45.0%
!! ACCAAGAGTCCAAGAGTATC+TGG + Chr8:79249200-79249219 MsG0880046867.01.T01:CDS 45.0%
!! TCCAAGAGTATCTGGACCTT+TGG + Chr8:79249208-79249227 MsG0880046867.01.T01:CDS 45.0%
ACGAACCGAATTGGTACCAC+GGG + Chr8:79248943-79248962 MsG0880046867.01.T01:CDS 50.0%
CACGAACCGAATTGGTACCA+CGG + Chr8:79248942-79248961 MsG0880046867.01.T01:CDS 50.0%
TACCACGGGCAAAAATGGAG+AGG + Chr8:79248957-79248976 MsG0880046867.01.T01:CDS 50.0%
TTCCCGCAATGAGCTTCAGT+TGG + Chr8:79248692-79248711 MsG0880046867.01.T01:CDS 50.0%
! TTCGGTTCGTGTAGAACCAC+AGG - Chr8:79248933-79248952 None:intergenic 50.0%
!! ATGGAGAGGAGCATTGGTCA+AGG + Chr8:79248971-79248990 MsG0880046867.01.T01:CDS 50.0%
GCTCATTGCGGGAAAAGACG+AGG - Chr8:79248686-79248705 None:intergenic 55.0%
!! CTCCTCTCCATTTTTGCCCG+TGG - Chr8:79248962-79248981 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 79248621 79249400 79248621 ID=MsG0880046867.01;Name=MsG0880046867.01
Chr8 mRNA 79248621 79249400 79248621 ID=MsG0880046867.01.T01;Parent=MsG0880046867.01;Name=MsG0880046867.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|259
Chr8 exon 79248621 79249400 79248621 ID=MsG0880046867.01.T01:exon:34256;Parent=MsG0880046867.01.T01
Chr8 CDS 79248621 79249400 79248621 ID=MsG0880046867.01.T01:cds;Parent=MsG0880046867.01.T01
Gene Sequence

>MsG0880046867.01.T01

ATGGACTGGTTTTCATGGTTATCAAAAACAACTCTTGACCATTCACTCATATATGATTATGGCCTCGTCTTTTCCCGCAATGAGCTTCAGTTGGAAGATGCAACTTACTTCAACCATGAGTTTCTACAAAGCATGGGAATCTCAATAGCCAAACACAGACTAGAGATTCTCAAACTAGTCAAAAAAGAACAAGAACTTGATCAACCATTACGACCAAAAAACCTTTCTGGTACCATCAAAAAGTGTCTAAAAAAATGCATGAGCAAGTTTGTTTTTCGCGACGATAGTGTTAAGAAAAACATGCCTTTACCTGTGGTTCTACACGAACCGAATTGGTACCACGGGCAAAAATGGAGAGGAGCATTGGTCAAGGAAGAGAAGCATCAGAAACCTATGTATAGAAGCAGAACAATGCCATATTCAGGACCATTAGATGGAAGAATGCATGAGCAGAAAATGGTCCAAAATAATAAAGCATTGAAGTTATCAGGACCAATTGATGGAAGATTGATGAATGAGAGAATGATGGTGTATACTAATAGAAGTCCATTAAGAAGTAGGGCTATTGATGGAAGATTCACCAAGAGTCCAAGAGTATCTGGACCTTTGGATGCAAAAATCATGTTTGAGAATAGAAGTCCAACTATTACAAGGAATTCTGTTGAAACTGATAGCCCTATGGGTTATAGTAGTCCTTATAATAAACCCAAAGGTGATTTTGATTTTGATGATGAGCATTCACTTTGGCCTACACTCTTTCAAGATCTTAATCCAACTTGA

Protein sequence

>MsG0880046867.01.T01

MDWFSWLSKTTLDHSLIYDYGLVFSRNELQLEDATYFNHEFLQSMGISIAKHRLEILKLVKKEQELDQPLRPKNLSGTIKKCLKKCMSKFVFRDDSVKKNMPLPVVLHEPNWYHGQKWRGALVKEEKHQKPMYRSRTMPYSGPLDGRMHEQKMVQNNKALKLSGPIDGRLMNERMMVYTNRSPLRSRAIDGRFTKSPRVSGPLDAKIMFENRSPTITRNSVETDSPMGYSSPYNKPKGDFDFDDEHSLWPTLFQDLNPT*