Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046867.01.T01 | XP_003609484.1 | 95.753 | 259 | 8 | 2 | 1 | 259 | 1 | 256 | 0 | 514 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047899.01 | MsG0880046867.01 | 0.804887 | 1.740912e-49 | 9.081778e-47 |
| MsG0080048065.01 | MsG0880046867.01 | 0.819703 | 1.010552e-52 | 7.834292e-50 |
| MsG0080048727.01 | MsG0880046867.01 | 0.845209 | 4.684551e-59 | 7.749571e-56 |
| MsG0080048773.01 | MsG0880046867.01 | 0.878454 | 2.771562e-69 | 1.448270e-65 |
| MsG0080048780.01 | MsG0880046867.01 | 0.841826 | 3.755356e-58 | 5.585052e-55 |
| MsG0080048801.01 | MsG0880046867.01 | 0.814796 | 1.283692e-51 | 8.697053e-49 |
| MsG0080048802.01 | MsG0880046867.01 | 0.868197 | 7.807016e-66 | 2.799850e-62 |
| MsG0080048845.01 | MsG0880046867.01 | 0.805839 | 1.099673e-49 | 5.878280e-47 |
| MsG0080048849.01 | MsG0880046867.01 | 0.825387 | 4.820664e-54 | 4.387387e-51 |
| MsG0080048876.01 | MsG0880046867.01 | 0.857203 | 1.915834e-62 | 4.691047e-59 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046867.01.T01 | MTR_4g116200 | 95.753 | 259 | 8 | 2 | 1 | 259 | 1 | 256 | 0.0 | 514 |
| MsG0880046867.01.T01 | MTR_2g450720 | 52.830 | 265 | 93 | 9 | 1 | 259 | 1 | 239 | 1.95e-82 | 247 |
| MsG0880046867.01.T01 | MTR_4g009760 | 48.667 | 150 | 68 | 4 | 1 | 142 | 1 | 149 | 8.13e-38 | 132 |
| MsG0880046867.01.T01 | MTR_2g104230 | 46.250 | 160 | 69 | 6 | 1 | 146 | 1 | 157 | 2.96e-33 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046867.01.T01 | AT2G12462 | 33.571 | 280 | 134 | 13 | 1 | 259 | 1 | 249 | 1.32e-33 | 122 |
| MsG0880046867.01.T01 | AT1G15760 | 39.157 | 166 | 87 | 3 | 3 | 162 | 5 | 162 | 4.82e-31 | 114 |
| MsG0880046867.01.T01 | AT1G80520 | 51.923 | 104 | 43 | 2 | 1 | 98 | 1 | 103 | 3.56e-29 | 109 |
Find 48 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAATGCATGAGCAGAAAA+TGG | 0.442330 | 8:+79249058 | MsG0880046867.01.T01:CDS |
| AAGGACTACTATAACCCATA+GGG | 0.607941 | 8:-79249295 | None:intergenic |
| AAGTTATCAGGACCAATTGA+TGG | 0.410093 | 8:+79249101 | MsG0880046867.01.T01:CDS |
| AATAAAGCATTGAAGTTATC+AGG | 0.300318 | 8:+79249089 | MsG0880046867.01.T01:CDS |
| ACAGAATTCCTTGTAATAGT+TGG | 0.363635 | 8:-79249260 | None:intergenic |
| ACCAAGAGTCCAAGAGTATC+TGG | 0.350418 | 8:+79249200 | MsG0880046867.01.T01:CDS |
| ACGAACCGAATTGGTACCAC+GGG | 0.566859 | 8:+79248943 | MsG0880046867.01.T01:CDS |
| ACTTCAATGCTTTATTATTT+TGG | 0.098715 | 8:-79249082 | None:intergenic |
| AGCAGAACAATGCCATATTC+AGG | 0.374090 | 8:+79249023 | MsG0880046867.01.T01:CDS |
| AGTCCTTATAATAAACCCAA+AGG | 0.617768 | 8:+79249311 | MsG0880046867.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAGAATTCCTTGTAATAGT+TGG | - | Chr8:79249263-79249282 | None:intergenic | 30.0% | |
| AGTCCTTATAATAAACCCAA+AGG | + | Chr8:79249311-79249330 | MsG0880046867.01.T01:CDS | 30.0% | |
| ATAGAAGTCCAACTATTACA+AGG | + | Chr8:79249252-79249271 | MsG0880046867.01.T01:CDS | 30.0% | |
| ATAGAAGTCCATTAAGAAGT+AGG | + | Chr8:79249159-79249178 | MsG0880046867.01.T01:CDS | 30.0% | |
| ATTGATGAATGAGAGAATGA+TGG | + | Chr8:79249127-79249146 | MsG0880046867.01.T01:CDS | 30.0% | |
| ATTGTTCTGCTTCTATACAT+AGG | - | Chr8:79249014-79249033 | None:intergenic | 30.0% | |
| TAGAAGTCCATTAAGAAGTA+GGG | + | Chr8:79249160-79249179 | MsG0880046867.01.T01:CDS | 30.0% | |
| TTCATCAATCTTCCATCAAT+TGG | - | Chr8:79249116-79249135 | None:intergenic | 30.0% | |
| AAGAATGCATGAGCAGAAAA+TGG | + | Chr8:79249058-79249077 | MsG0880046867.01.T01:CDS | 35.0% | |
| AAGGACTACTATAACCCATA+GGG | - | Chr8:79249298-79249317 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 79248621 | 79249400 | 79248621 | ID=MsG0880046867.01;Name=MsG0880046867.01 |
| Chr8 | mRNA | 79248621 | 79249400 | 79248621 | ID=MsG0880046867.01.T01;Parent=MsG0880046867.01;Name=MsG0880046867.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|259 |
| Chr8 | exon | 79248621 | 79249400 | 79248621 | ID=MsG0880046867.01.T01:exon:34256;Parent=MsG0880046867.01.T01 |
| Chr8 | CDS | 79248621 | 79249400 | 79248621 | ID=MsG0880046867.01.T01:cds;Parent=MsG0880046867.01.T01 |
| Gene Sequence |
>MsG0880046867.01.T01 ATGGACTGGTTTTCATGGTTATCAAAAACAACTCTTGACCATTCACTCATATATGATTATGGCCTCGTCTTTTCCCGCAATGAGCTTCAGTTGGAAGATGCAACTTACTTCAACCATGAGTTTCTACAAAGCATGGGAATCTCAATAGCCAAACACAGACTAGAGATTCTCAAACTAGTCAAAAAAGAACAAGAACTTGATCAACCATTACGACCAAAAAACCTTTCTGGTACCATCAAAAAGTGTCTAAAAAAATGCATGAGCAAGTTTGTTTTTCGCGACGATAGTGTTAAGAAAAACATGCCTTTACCTGTGGTTCTACACGAACCGAATTGGTACCACGGGCAAAAATGGAGAGGAGCATTGGTCAAGGAAGAGAAGCATCAGAAACCTATGTATAGAAGCAGAACAATGCCATATTCAGGACCATTAGATGGAAGAATGCATGAGCAGAAAATGGTCCAAAATAATAAAGCATTGAAGTTATCAGGACCAATTGATGGAAGATTGATGAATGAGAGAATGATGGTGTATACTAATAGAAGTCCATTAAGAAGTAGGGCTATTGATGGAAGATTCACCAAGAGTCCAAGAGTATCTGGACCTTTGGATGCAAAAATCATGTTTGAGAATAGAAGTCCAACTATTACAAGGAATTCTGTTGAAACTGATAGCCCTATGGGTTATAGTAGTCCTTATAATAAACCCAAAGGTGATTTTGATTTTGATGATGAGCATTCACTTTGGCCTACACTCTTTCAAGATCTTAATCCAACTTGA |
| Protein sequence |
>MsG0880046867.01.T01 MDWFSWLSKTTLDHSLIYDYGLVFSRNELQLEDATYFNHEFLQSMGISIAKHRLEILKLVKKEQELDQPLRPKNLSGTIKKCLKKCMSKFVFRDDSVKKNMPLPVVLHEPNWYHGQKWRGALVKEEKHQKPMYRSRTMPYSGPLDGRMHEQKMVQNNKALKLSGPIDGRLMNERMMVYTNRSPLRSRAIDGRFTKSPRVSGPLDAKIMFENRSPTITRNSVETDSPMGYSSPYNKPKGDFDFDDEHSLWPTLFQDLNPT* |
