Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016690.01.T01 | XP_003602442.1 | 97.436 | 117 | 3 | 0 | 1 | 117 | 1 | 117 | 2.02E-75 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016690.01.T01 | Q9FIR9 | 48.571 | 105 | 39 | 3 | 12 | 113 | 1 | 93 | 6.11E-22 | 85.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016690.01.T01 | G7J9Q1 | 97.436 | 117 | 3 | 0 | 1 | 117 | 1 | 117 | 9.63e-76 | 229 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380016690.01 | MsG0480022248.01 | 0.806563 | 7.743025e-50 | 4.216082e-47 |
| MsG0380016690.01 | MsG0780038631.01 | 0.800568 | 1.356230e-48 | 6.335665e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016690.01.T01 | MTR_3g093400 | 97.436 | 117 | 3 | 0 | 1 | 117 | 1 | 117 | 2.44e-79 | 229 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016690.01.T01 | AT5G24660 | 48.571 | 105 | 39 | 3 | 12 | 113 | 1 | 93 | 6.22e-23 | 85.9 |
| MsG0380016690.01.T01 | AT5G24655 | 48.485 | 99 | 36 | 3 | 12 | 107 | 1 | 87 | 3.40e-21 | 81.3 |
| MsG0380016690.01.T01 | AT3G49570 | 52.083 | 96 | 37 | 2 | 12 | 107 | 1 | 87 | 1.89e-19 | 77.0 |
| MsG0380016690.01.T01 | AT3G49580 | 52.632 | 76 | 29 | 1 | 29 | 104 | 16 | 84 | 1.99e-19 | 77.0 |
| MsG0380016690.01.T01 | AT3G49580 | 71.053 | 38 | 11 | 0 | 67 | 104 | 28 | 65 | 1.41e-12 | 58.9 |
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAGAGTTGAAGGAGAGT+AGG | 0.397427 | 3:-86619372 | MsG0380016690.01.T01:CDS |
| GCTGCGCGTGGCGGAGGAAG+CGG | 0.401471 | 3:-86619302 | MsG0380016690.01.T01:CDS |
| GAAAGAGTTGAAGGAGAGTA+GGG | 0.413600 | 3:-86619371 | MsG0380016690.01.T01:CDS |
| CTTCTCTTCAGCTCCGACTC+CGG | 0.438820 | 3:+86619409 | None:intergenic |
| CAATCGTATTGTTTCTCTGA+TGG | 0.446813 | 3:-86619212 | MsG0380016690.01.T01:CDS |
| GGAAAGGCTGCGCGTGGCGG+AGG | 0.464821 | 3:-86619308 | MsG0380016690.01.T01:CDS |
| TCAGCTTGAGAAAGAGTTGA+AGG | 0.470244 | 3:-86619380 | MsG0380016690.01.T01:CDS |
| ATGCTCACAGCTCGGAGAGC+TGG | 0.481510 | 3:-86619269 | MsG0380016690.01.T01:CDS |
| GTGGGAAAGGCTGCGCGTGG+CGG | 0.509515 | 3:-86619311 | MsG0380016690.01.T01:CDS |
| AGTTACAGAGCGCGTGGGAA+AGG | 0.511401 | 3:-86619324 | MsG0380016690.01.T01:CDS |
| TGCGGTGCCGGTGCCGGAGT+CGG | 0.513302 | 3:-86619422 | MsG0380016690.01.T01:CDS |
| GTGCCGATCACTGCCATCAT+CGG | 0.537295 | 3:+86619469 | None:intergenic |
| CGCGTGGGAAAGGCTGCGCG+TGG | 0.538078 | 3:-86619314 | MsG0380016690.01.T01:CDS |
| TTCAGCTCCGACTCCGGCAC+CGG | 0.539404 | 3:+86619415 | None:intergenic |
| TCACCGATGATGGCAGTGAT+CGG | 0.571734 | 3:-86619472 | MsG0380016690.01.T01:CDS |
| CAAAACCAATCACAACGAGT+TGG | 0.575914 | 3:+86619147 | None:intergenic |
| ATGATGGCAGTGATCGGCAC+CGG | 0.580016 | 3:-86619466 | MsG0380016690.01.T01:CDS |
| GCGCGTGGCGGAGGAAGCGG+AGG | 0.588927 | 3:-86619299 | MsG0380016690.01.T01:CDS |
| GACGGCAGTTACAGAGCGCG+TGG | 0.590880 | 3:-86619330 | MsG0380016690.01.T01:CDS |
| AGAGAGAAGAGCAAATGAGA+CGG | 0.606848 | 3:-86619348 | MsG0380016690.01.T01:CDS |
| TGGCGGAGGAAGCGGAGGAG+AGG | 0.608333 | 3:-86619294 | MsG0380016690.01.T01:CDS |
| ATGGCAGTGATCGGCACCGG+AGG | 0.608782 | 3:-86619463 | MsG0380016690.01.T01:CDS |
| GAGAGGTTATGCTCACAGCT+CGG | 0.627892 | 3:-86619277 | MsG0380016690.01.T01:CDS |
| ACGGCAGTTACAGAGCGCGT+GGG | 0.692882 | 3:-86619329 | MsG0380016690.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAGAGTCTAAAAATG+CGG | - | Chr3:86619203-86619222 | MsG0380016690.01.T01:CDS | 20.0% |
| ! | TATTTTCATTTCACCGATGA+TGG | - | Chr3:86619161-86619180 | MsG0380016690.01.T01:CDS | 30.0% |
| !!! | TTTAGACTCTTTTTTTCCTC+CGG | + | Chr3:86619199-86619218 | None:intergenic | 30.0% |
| ! | CAATCGTATTGTTTCTCTGA+TGG | - | Chr3:86619431-86619450 | MsG0380016690.01.T01:CDS | 35.0% |
| AGAAAGAGTTGAAGGAGAGT+AGG | - | Chr3:86619271-86619290 | MsG0380016690.01.T01:CDS | 40.0% | |
| AGAGAGAAGAGCAAATGAGA+CGG | - | Chr3:86619295-86619314 | MsG0380016690.01.T01:CDS | 40.0% | |
| GAAAGAGTTGAAGGAGAGTA+GGG | - | Chr3:86619272-86619291 | MsG0380016690.01.T01:CDS | 40.0% | |
| TCAGCTTGAGAAAGAGTTGA+AGG | - | Chr3:86619263-86619282 | MsG0380016690.01.T01:CDS | 40.0% | |
| AGAGTCTAAAAATGCGGTGC+CGG | - | Chr3:86619209-86619228 | MsG0380016690.01.T01:CDS | 45.0% | |
| GAGAGGTTATGCTCACAGCT+CGG | - | Chr3:86619366-86619385 | MsG0380016690.01.T01:CDS | 50.0% | |
| ! | TCACCGATGATGGCAGTGAT+CGG | - | Chr3:86619171-86619190 | MsG0380016690.01.T01:CDS | 50.0% |
| AGTTACAGAGCGCGTGGGAA+AGG | - | Chr3:86619319-86619338 | MsG0380016690.01.T01:CDS | 55.0% | |
| ATGATGGCAGTGATCGGCAC+CGG | - | Chr3:86619177-86619196 | MsG0380016690.01.T01:CDS | 55.0% | |
| CTTCTCTTCAGCTCCGACTC+CGG | + | Chr3:86619237-86619256 | None:intergenic | 55.0% | |
| ! | GTGCCGATCACTGCCATCAT+CGG | + | Chr3:86619177-86619196 | None:intergenic | 55.0% |
| !! | TAAAAATGCGGTGCCGGTGC+CGG | - | Chr3:86619215-86619234 | MsG0380016690.01.T01:CDS | 55.0% |
| ACGGCAGTTACAGAGCGCGT+GGG | - | Chr3:86619314-86619333 | MsG0380016690.01.T01:CDS | 60.0% | |
| ATGCTCACAGCTCGGAGAGC+TGG | - | Chr3:86619374-86619393 | MsG0380016690.01.T01:CDS | 60.0% | |
| GACGGCAGTTACAGAGCGCG+TGG | - | Chr3:86619313-86619332 | MsG0380016690.01.T01:CDS | 65.0% | |
| TTCAGCTCCGACTCCGGCAC+CGG | + | Chr3:86619231-86619250 | None:intergenic | 65.0% | |
| ! | ATGGCAGTGATCGGCACCGG+AGG | - | Chr3:86619180-86619199 | MsG0380016690.01.T01:CDS | 65.0% |
| GTGGGAAAGGCTGCGCGTGG+CGG | - | Chr3:86619332-86619351 | MsG0380016690.01.T01:CDS | 70.0% | |
| TGGCGGAGGAAGCGGAGGAG+AGG | - | Chr3:86619349-86619368 | MsG0380016690.01.T01:CDS | 70.0% | |
| CGCGTGGGAAAGGCTGCGCG+TGG | - | Chr3:86619329-86619348 | MsG0380016690.01.T01:CDS | 75.0% | |
| GGAAAGGCTGCGCGTGGCGG+AGG | - | Chr3:86619335-86619354 | MsG0380016690.01.T01:CDS | 75.0% | |
| ! | GCTGCGCGTGGCGGAGGAAG+CGG | - | Chr3:86619341-86619360 | MsG0380016690.01.T01:CDS | 75.0% |
| !! | TGCGGTGCCGGTGCCGGAGT+CGG | - | Chr3:86619221-86619240 | MsG0380016690.01.T01:CDS | 75.0% |
| !! | GCGCGTGGCGGAGGAAGCGG+AGG | - | Chr3:86619344-86619363 | MsG0380016690.01.T01:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 86619156 | 86619509 | 86619156 | ID=MsG0380016690.01;Name=MsG0380016690.01 |
| Chr3 | mRNA | 86619156 | 86619509 | 86619156 | ID=MsG0380016690.01.T01;Parent=MsG0380016690.01;Name=MsG0380016690.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|117 |
| Chr3 | exon | 86619156 | 86619509 | 86619156 | ID=MsG0380016690.01.T01:exon:6689;Parent=MsG0380016690.01.T01 |
| Chr3 | CDS | 86619156 | 86619509 | 86619156 | ID=MsG0380016690.01.T01:cds;Parent=MsG0380016690.01.T01 |
| Gene Sequence |
| Protein sequence |
