AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022248.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022248.01.T01 MTR_4g082860 91.200 125 8 1 1 122 1 125 7.33e-68 200
MsG0480022248.01.T01 MTR_4g082830 66.667 132 31 4 3 122 11 141 2.31e-40 131
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022248.01.T01 AT2G03440 42.636 129 52 5 11 122 64 187 4.74e-14 65.5
MsG0480022248.01.T01 AT1G13930 48.062 129 47 8 8 122 33 155 2.28e-13 63.2
MsG0480022248.01.T01 AT1G13930 48.062 129 47 8 8 122 33 155 2.28e-13 63.2
MsG0480022248.01.T01 AT1G13930 48.062 129 47 8 8 122 33 155 2.28e-13 63.2

Find 26 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCAGCACAGTCAGGTTTC+GGG 0.304942 4:-70086045 MsG0480022248.01.T01:CDS
GAGGCAGCACAGTCAGGTTT+CGG 0.309235 4:-70086046 MsG0480022248.01.T01:CDS
CCACCTGATCCTCCACCTTC+AGG 0.354191 4:+70085815 None:intergenic
GGTGGAGGATCAGGTGGGCT+TGG 0.384709 4:-70085809 MsG0480022248.01.T01:CDS
AGTTGGTCAGTATGCTAAAT+TGG 0.398975 4:-70085969 MsG0480022248.01.T01:CDS
TGCACTTGCCATTAGCTCAC+TGG 0.413536 4:+70086081 None:intergenic
GATTTCAACCAGTGAGCTAA+TGG 0.416458 4:-70086089 MsG0480022248.01.T01:CDS
GGAGGATCAGGTGGGCTTGG+TGG 0.418763 4:-70085806 MsG0480022248.01.T01:CDS
AAGCCTGAAGGTGGAGGATC+AGG 0.423090 4:-70085818 MsG0480022248.01.T01:CDS
AGCTGCTGATTATCTGCATA+AGG 0.469713 4:-70085912 MsG0480022248.01.T01:CDS
GACAAGACAGCAGAAGCTGC+TGG 0.526359 4:-70086007 MsG0480022248.01.T01:CDS
GTAGCAGAGGCAGCACAGTC+AGG 0.527765 4:-70086052 MsG0480022248.01.T01:CDS
TGATCTGCAGGTTTAGAAGC+TGG 0.532370 4:+70085863 None:intergenic
AAGGTTGAGAACACTACTAC+TGG 0.547092 4:-70085893 MsG0480022248.01.T01:CDS
AAGTGCAAAGGTGGTAGCAG+AGG 0.549500 4:-70086065 MsG0480022248.01.T01:CDS
TTGGATGAACAGAAAGGTGT+AGG 0.558648 4:-70085950 MsG0480022248.01.T01:CDS
GCTAAATTGGATGAACAGAA+AGG 0.567227 4:-70085956 MsG0480022248.01.T01:CDS
CCTGAAGGTGGAGGATCAGG+TGG 0.574520 4:-70085815 MsG0480022248.01.T01:CDS
GGTGATCTTCTAGATGCAGT+TGG 0.590308 4:-70085986 MsG0480022248.01.T01:CDS
TGAGCTAATGGCAAGTGCAA+AGG 0.603559 4:-70086077 MsG0480022248.01.T01:CDS
GCTAATGGCAAGTGCAAAGG+TGG 0.615992 4:-70086074 MsG0480022248.01.T01:CDS
GTTGCTAAGCCTGAAGGTGG+AGG 0.637775 4:-70085824 MsG0480022248.01.T01:CDS
TCTGCAGGTTTAGAAGCTGG+TGG 0.662750 4:+70085866 None:intergenic
GATGATGTTGCTAAGCCTGA+AGG 0.667542 4:-70085830 MsG0480022248.01.T01:CDS
GATGTTGCTAAGCCTGAAGG+TGG 0.674394 4:-70085827 MsG0480022248.01.T01:CDS
CTGAAGGTGGAGGATCAGGT+GGG 0.684433 4:-70085814 MsG0480022248.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGCAACATCATCACTTTT+AGG + Chr4:70086048-70086067 None:intergenic 30.0%
AGTTGGTCAGTATGCTAAAT+TGG - Chr4:70085915-70085934 MsG0480022248.01.T01:CDS 35.0%
GCTAAATTGGATGAACAGAA+AGG - Chr4:70085928-70085947 MsG0480022248.01.T01:CDS 35.0%
AAGGTTGAGAACACTACTAC+TGG - Chr4:70085991-70086010 MsG0480022248.01.T01:CDS 40.0%
GATTTCAACCAGTGAGCTAA+TGG - Chr4:70085795-70085814 MsG0480022248.01.T01:CDS 40.0%
! AGCTGCTGATTATCTGCATA+AGG - Chr4:70085972-70085991 MsG0480022248.01.T01:CDS 40.0%
! TTGGATGAACAGAAAGGTGT+AGG - Chr4:70085934-70085953 MsG0480022248.01.T01:CDS 40.0%
GATGATGTTGCTAAGCCTGA+AGG - Chr4:70086054-70086073 MsG0480022248.01.T01:CDS 45.0%
GGTGATCTTCTAGATGCAGT+TGG - Chr4:70085898-70085917 MsG0480022248.01.T01:CDS 45.0%
TGAGCTAATGGCAAGTGCAA+AGG - Chr4:70085807-70085826 MsG0480022248.01.T01:CDS 45.0%
TGATCTGCAGGTTTAGAAGC+TGG + Chr4:70086024-70086043 None:intergenic 45.0%
! GATTTTGCTAAGGTTGCTGG+AGG - Chr4:70086102-70086121 MsG0480022248.01.T01:CDS 45.0%
! GGTGATTTTGCTAAGGTTGC+TGG - Chr4:70086099-70086118 MsG0480022248.01.T01:CDS 45.0%
!! TCACTTTTAGGCTGATCTGC+AGG + Chr4:70086036-70086055 None:intergenic 45.0%
!!! GCTTGGTGGTGATTTTGCTA+AGG - Chr4:70086092-70086111 MsG0480022248.01.T01:CDS 45.0%
AAGTGCAAAGGTGGTAGCAG+AGG - Chr4:70085819-70085838 MsG0480022248.01.T01:CDS 50.0%
GATGTTGCTAAGCCTGAAGG+TGG - Chr4:70086057-70086076 MsG0480022248.01.T01:CDS 50.0%
GCTAATGGCAAGTGCAAAGG+TGG - Chr4:70085810-70085829 MsG0480022248.01.T01:CDS 50.0%
TCTGCAGGTTTAGAAGCTGG+TGG + Chr4:70086021-70086040 None:intergenic 50.0%
TGCACTTGCCATTAGCTCAC+TGG + Chr4:70085806-70085825 None:intergenic 50.0%
AAGCCTGAAGGTGGAGGATC+AGG - Chr4:70086066-70086085 MsG0480022248.01.T01:CDS 55.0%
AGGCAGCACAGTCAGGTTTC+GGG - Chr4:70085839-70085858 MsG0480022248.01.T01:CDS 55.0%
CTGAAGGTGGAGGATCAGGT+GGG - Chr4:70086070-70086089 MsG0480022248.01.T01:CDS 55.0%
GACAAGACAGCAGAAGCTGC+TGG - Chr4:70085877-70085896 MsG0480022248.01.T01:CDS 55.0%
GAGGCAGCACAGTCAGGTTT+CGG - Chr4:70085838-70085857 MsG0480022248.01.T01:CDS 55.0%
GTTGCTAAGCCTGAAGGTGG+AGG - Chr4:70086060-70086079 MsG0480022248.01.T01:CDS 55.0%
CCACCTGATCCTCCACCTTC+AGG + Chr4:70086072-70086091 None:intergenic 60.0%
CCTGAAGGTGGAGGATCAGG+TGG - Chr4:70086069-70086088 MsG0480022248.01.T01:CDS 60.0%
GTAGCAGAGGCAGCACAGTC+AGG - Chr4:70085832-70085851 MsG0480022248.01.T01:CDS 60.0%
! GGAGGATCAGGTGGGCTTGG+TGG - Chr4:70086078-70086097 MsG0480022248.01.T01:CDS 65.0%
! GGTGGAGGATCAGGTGGGCT+TGG - Chr4:70086075-70086094 MsG0480022248.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 70085769 70086137 70085769 ID=MsG0480022248.01;Name=MsG0480022248.01
Chr4 mRNA 70085769 70086137 70085769 ID=MsG0480022248.01.T01;Parent=MsG0480022248.01;Name=MsG0480022248.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|122
Chr4 exon 70085769 70086137 70085769 ID=MsG0480022248.01.T01:exon:11103;Parent=MsG0480022248.01.T01
Chr4 CDS 70085769 70086137 70085769 ID=MsG0480022248.01.T01:cds;Parent=MsG0480022248.01.T01
Gene Sequence

>MsG0480022248.01.T01

ATGGCAGCAAGTGGAGAAGAGAAGAAGATTTCAACCAGTGAGCTAATGGCAAGTGCAAAGGTGGTAGCAGAGGCAGCACAGTCAGGTTTCGGGAAAGATTTAGACAAAGACAAGACAGCAGAAGCTGCTGGTGATCTTCTAGATGCAGTTGGTCAGTATGCTAAATTGGATGAACAGAAAGGTGTAGGATCATACGTTGATAAAGCTGCTGATTATCTGCATAAGGTTGAGAACACTACTACTGGTGCTCCACCAGCTTCTAAACCTGCAGATCAGCCTAAAAGTGATGATGTTGCTAAGCCTGAAGGTGGAGGATCAGGTGGGCTTGGTGGTGATTTTGCTAAGGTTGCTGGAGGTTTCTTTAAATGA

Protein sequence

>MsG0480022248.01.T01

MAASGEEKKISTSELMASAKVVAEAAQSGFGKDLDKDKTAEAAGDLLDAVGQYAKLDEQKGVGSYVDKAADYLHKVENTTTGAPPASKPADQPKSDDVAKPEGGGSGGLGGDFAKVAGGFFK*