AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016977.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016977.01.T01 MTR_3g098820 96.875 64 2 0 140 203 15 78 4.05e-41 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016977.01.T01 AT5G50810 75.000 60 15 0 142 201 17 76 2.03e-24 92.0

Find 54 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTAATATTTAGCAAATTT+TGG 0.095082 3:-90246519 MsG0380016977.01.T01:three_prime_UTR
AAATGTAATCGAGTTGAAAA+AGG 0.324452 3:+90247599 None:intergenic
ACGCCGGCAAATGGTCTTTC+CGG 0.336710 3:-90248639 MsG0380016977.01.T01:CDS
TCCGATATATGCCACTTATA+TGG 0.351533 3:-90246864 MsG0380016977.01.T01:intron
TATGGCTTAGGGGATCTAAA+TGG 0.373832 3:-90246920 MsG0380016977.01.T01:intron
ACCATATAAGTGGCATATAT+CGG 0.381849 3:+90246863 None:intergenic
CACATATAAGTGGTATATAT+CGG 0.393400 3:+90246893 None:intergenic
AAGAGCCATGATTAATGAAA+TGG 0.403854 3:-90246751 MsG0380016977.01.T01:CDS
TGCCACCATTTCATTAATCA+TGG 0.406262 3:+90246746 None:intergenic
AAATGCATCACAGGCACGCC+TGG 0.412006 3:-90246699 MsG0380016977.01.T01:CDS
AATCCATGGCCATCTGGTTA+AGG 0.439237 3:-90248674 None:intergenic
CTGTTAGTGCTAAGGCCAGT+TGG 0.443465 3:-90247332 MsG0380016977.01.T01:CDS
CCTGGGAATAAGTTCAGTTC+CGG 0.455891 3:-90246681 MsG0380016977.01.T01:CDS
CCGCCGGAAAGACCATTTGC+CGG 0.465627 3:+90248636 None:intergenic
CGGCGGAGTGAGATGGTGTC+AGG 0.467898 3:-90248619 MsG0380016977.01.T01:CDS
AGTGAATCAGAGGGAGGCAG+AGG 0.472649 3:-90248573 MsG0380016977.01.T01:CDS
TCGGCGAATCCATGGCCATC+TGG 0.472735 3:-90248680 None:intergenic
CAAAGATGACTAGTCAATGC+TGG 0.492799 3:-90246725 MsG0380016977.01.T01:CDS
GAGGCAGAGGACAAGGACGC+AGG 0.500362 3:-90248560 MsG0380016977.01.T01:intron
AGATTGAAATACCATATAAG+TGG 0.500622 3:+90246853 None:intergenic
TTACTGCTTGTTTGACTGAA+GGG 0.501469 3:-90246403 MsG0380016977.01.T01:three_prime_UTR
GTTGTGAGAACGCCGGCAAA+TGG 0.504295 3:-90248648 MsG0380016977.01.T01:CDS
ACACTGTGCACAGCGTTACA+TGG 0.508169 3:-90246640 MsG0380016977.01.T01:CDS
TCAGGAGAGAATGGCGGCGA+GGG 0.513895 3:-90248601 MsG0380016977.01.T01:CDS
AACGCTGTGCACAGTGTGTT+AGG 0.520352 3:+90246646 None:intergenic
GAGATGGTGTCAGGAGAGAA+TGG 0.521088 3:-90248610 MsG0380016977.01.T01:CDS
CCATGGCCATCTGGTTAAGG+TGG 0.524953 3:-90248671 MsG0380016977.01.T01:CDS
TGCTGGGACAAATGCATCAC+AGG 0.525844 3:-90246708 MsG0380016977.01.T01:CDS
TGAGCATGCTTATCATGAAA+CGG 0.531078 3:-90246614 MsG0380016977.01.T01:CDS
CGGAATATGCCACATATAAG+TGG 0.531179 3:+90246883 None:intergenic
GTCTTTCCGGCGGAGTGAGA+TGG 0.537564 3:-90248626 MsG0380016977.01.T01:CDS
CTTACTGCTTGTTTGACTGA+AGG 0.539908 3:-90246404 MsG0380016977.01.T01:three_prime_UTR
TCAGAGGGAGGCAGAGGACA+AGG 0.545836 3:-90248567 MsG0380016977.01.T01:CDS
TTAATTAAGAAAGCACATCA+AGG 0.547393 3:+90246563 None:intergenic
CCGGAACTGAACTTATTCCC+AGG 0.566395 3:+90246681 None:intergenic
GAGGGAGAGAAGTGAATCAG+AGG 0.570113 3:-90248583 MsG0380016977.01.T01:CDS
AAAGATGACTAGTCAATGCT+GGG 0.584821 3:-90246724 MsG0380016977.01.T01:CDS
CGATATATACCACTTATATG+TGG 0.590415 3:-90246892 MsG0380016977.01.T01:CDS
ATACTGTTCTGTTAGTGCTA+AGG 0.595621 3:-90247340 MsG0380016977.01.T01:CDS
CTGACACCATCTCACTCCGC+CGG 0.596439 3:+90248620 None:intergenic
AATGCATCACAGGCACGCCT+GGG 0.596603 3:-90246698 MsG0380016977.01.T01:CDS
TGTTAGGCAATTAGTCTCAC+CGG 0.596955 3:+90246662 None:intergenic
AGGGAGAGAAGTGAATCAGA+GGG 0.601619 3:-90248582 MsG0380016977.01.T01:CDS
CCACCTTAACCAGATGGCCA+TGG 0.606396 3:+90248671 None:intergenic
AGCCATGATTAATGAAATGG+TGG 0.612893 3:-90246748 MsG0380016977.01.T01:CDS
CATGGCCATCTGGTTAAGGT+GGG 0.621271 3:-90248670 MsG0380016977.01.T01:CDS
GTCAGGAGAGAATGGCGGCG+AGG 0.623602 3:-90248602 MsG0380016977.01.T01:CDS
CACAACCCACCTTAACCAGA+TGG 0.626710 3:+90248665 None:intergenic
GAGTATGCAATGAAGATCAA+CGG 0.630450 3:-90246586 MsG0380016977.01.T01:exon
CCGGCAAATGGTCTTTCCGG+CGG 0.651106 3:-90248636 MsG0380016977.01.T01:CDS
GAGAGAAGTGAATCAGAGGG+AGG 0.656529 3:-90248579 MsG0380016977.01.T01:CDS
GATGAAAAGCTCAGTGCATG+TGG 0.677105 3:-90246434 MsG0380016977.01.T01:three_prime_UTR
ATGGTGTCAGGAGAGAATGG+CGG 0.721835 3:-90248607 MsG0380016977.01.T01:CDS
AAGGTGGGTTGTGAGAACGC+CGG 0.760296 3:-90248655 MsG0380016977.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATTGAAATTATTACA+GGG - Chr3:90246928-90246947 MsG0380016977.01.T01:intron 10.0%
!! TATATAATTGAAATTATTAC+AGG - Chr3:90246927-90246946 MsG0380016977.01.T01:intron 10.0%
!!! TTTAAAATGTTTTTTAAGTT+AGG - Chr3:90247057-90247076 MsG0380016977.01.T01:intron 10.0%
!! AAGAACAAAAATTATTAATG+TGG - Chr3:90248632-90248651 MsG0380016977.01.T01:CDS 15.0%
!! AGAACAAAAATTATTAATGT+GGG - Chr3:90248633-90248652 MsG0380016977.01.T01:CDS 15.0%
!! TTTGATATTTAAAAGTGATT+TGG - Chr3:90247704-90247723 MsG0380016977.01.T01:intron 15.0%
!!! ATTTTTTTCCATAAGATAAA+GGG + Chr3:90247825-90247844 None:intergenic 15.0%
!!! TATTTTTTTCCATAAGATAA+AGG + Chr3:90247826-90247845 None:intergenic 15.0%
!!! TCTAAAACGTATTTTTATTT+TGG + Chr3:90246754-90246773 None:intergenic 15.0%
!! ATTATCATTATTATGGCTTA+GGG - Chr3:90248053-90248072 MsG0380016977.01.T01:intron 20.0%
!! TATATAATTTGAACCATATG+TGG + Chr3:90246913-90246932 None:intergenic 20.0%
!! TCTAATAATAGTTGTAACTA+TGG - Chr3:90247874-90247893 MsG0380016977.01.T01:intron 20.0%
!! TGTGTAAAGTAAAAAAAAGA+CGG - Chr3:90246871-90246890 MsG0380016977.01.T01:CDS 20.0%
!!! AAATATACAACTGAATCTTT+TGG + Chr3:90246805-90246824 None:intergenic 20.0%
!!! CGTTAATATTTAGCAAATTT+TGG - Chr3:90248465-90248484 MsG0380016977.01.T01:intron 20.0%
!!! TTTTATAGAAGTATAAGTCA+AGG + Chr3:90246692-90246711 None:intergenic 20.0%
!!! TTTTTTTCCATAAGATAAAG+GGG + Chr3:90247824-90247843 None:intergenic 20.0%
! AAGAAGAAAAAGAGAAAAGA+TGG + Chr3:90247150-90247169 None:intergenic 25.0%
! AATAGTTGTAACTATGGAAT+TGG - Chr3:90247880-90247899 MsG0380016977.01.T01:intron 25.0%
! AATAGTTTAGGCAAAAGTTA+TGG + Chr3:90246663-90246682 None:intergenic 25.0%
! AGATTGAAATACCATATAAG+TGG + Chr3:90248134-90248153 None:intergenic 25.0%
! CACATATAAGTGGTATATAT+CGG + Chr3:90248094-90248113 None:intergenic 25.0%
! CATTATCATTATTATGGCTT+AGG - Chr3:90248052-90248071 MsG0380016977.01.T01:intron 25.0%
! CTAAGCCATAATAATGATAA+TGG + Chr3:90248054-90248073 None:intergenic 25.0%
! GGTTGTTATATTTCATTACA+GGG + Chr3:90248179-90248198 None:intergenic 25.0%
! GTTGTTATATTTCATTACAG+GGG + Chr3:90248178-90248197 None:intergenic 25.0%
! TACACATTAGACAATTTAGA+AGG + Chr3:90246857-90246876 None:intergenic 25.0%
! TATAAAATTCGAAAAGTCGT+AGG - Chr3:90246991-90247010 MsG0380016977.01.T01:intron 25.0%
! TATAGAAAACCCTAAACTTA+AGG + Chr3:90247176-90247195 None:intergenic 25.0%
! TCAAAATTCATAAGACAGAA+GGG - Chr3:90246720-90246739 MsG0380016977.01.T01:CDS 25.0%
! TTATCATTATTATGGCTTAG+GGG - Chr3:90248054-90248073 MsG0380016977.01.T01:intron 25.0%
!! CTTCTTTTTCCTTAAGTTTA+GGG - Chr3:90247164-90247183 MsG0380016977.01.T01:intron 25.0%
!! TCTTCTTTTTCCTTAAGTTT+AGG - Chr3:90247163-90247182 MsG0380016977.01.T01:intron 25.0%
!! TTAATTAAGAAAGCACATCA+AGG + Chr3:90248424-90248443 None:intergenic 25.0%
!! TTTAAGTTAGGTGATGATTT+GGG - Chr3:90247069-90247088 MsG0380016977.01.T01:intron 25.0%
!!! AAATGTAATCGAGTTGAAAA+AGG + Chr3:90247388-90247407 None:intergenic 25.0%
!!! AATGTAATCGAGTTGAAAAA+GGG + Chr3:90247387-90247406 None:intergenic 25.0%
!!! TTTTAAGTTAGGTGATGATT+TGG - Chr3:90247068-90247087 MsG0380016977.01.T01:intron 25.0%
AAGAGCCATGATTAATGAAA+TGG - Chr3:90248233-90248252 MsG0380016977.01.T01:intron 30.0%
ACCATATAAGTGGCATATAT+CGG + Chr3:90248124-90248143 None:intergenic 30.0%
AGTATCTGTTAATCGTAAGA+AGG + Chr3:90247364-90247383 None:intergenic 30.0%
ATAATTTGAACCATATGTGG+AGG + Chr3:90246910-90246929 None:intergenic 30.0%
ATTCTTTGGATGAAATCTGA+TGG - Chr3:90246593-90246612 MsG0380016977.01.T01:exon 30.0%
CACTGCCATTATCATTATTA+TGG - Chr3:90248046-90248065 MsG0380016977.01.T01:intron 30.0%
CGATATATACCACTTATATG+TGG - Chr3:90248092-90248111 MsG0380016977.01.T01:intron 30.0%
GGGTTGTTATATTTCATTAC+AGG + Chr3:90248180-90248199 None:intergenic 30.0%
GTCAAAATTCATAAGACAGA+AGG - Chr3:90246719-90246738 MsG0380016977.01.T01:CDS 30.0%
TAACAGTTATCACTAAGACA+AGG + Chr3:90247523-90247542 None:intergenic 30.0%
TAATTTGAACCATATGTGGA+GGG + Chr3:90246909-90246928 None:intergenic 30.0%
! TATCAATTTTATGGTGGCTA+AGG - Chr3:90247581-90247600 MsG0380016977.01.T01:CDS 30.0%
! TTTTATATTTTGTCGAGACG+TGG - Chr3:90248493-90248512 MsG0380016977.01.T01:intron 30.0%
!! AGATTTGATTTTTCATGCTG+AGG - Chr3:90247557-90247576 MsG0380016977.01.T01:intron 30.0%
!! ATAGGTTTTTAAGAGAGACA+TGG + Chr3:90247954-90247973 None:intergenic 30.0%
!! CATAATAATGATAATGGCAG+TGG + Chr3:90248048-90248067 None:intergenic 30.0%
AAGAAGACCCCTTTATCTTA+TGG - Chr3:90247814-90247833 MsG0380016977.01.T01:intron 35.0%
ACAAAGCTACAGTAGACTAT+AGG + Chr3:90247972-90247991 None:intergenic 35.0%
AGCCATGATTAATGAAATGG+TGG - Chr3:90248236-90248255 MsG0380016977.01.T01:intron 35.0%
ATACTGTTCTGTTAGTGCTA+AGG - Chr3:90247644-90247663 MsG0380016977.01.T01:intron 35.0%
ATGTTTAACACCCAAGCAAT+CGG - Chr3:90247094-90247113 MsG0380016977.01.T01:intron 35.0%
ATGTTTGACTACATTTCCGT+TGG - Chr3:90247770-90247789 MsG0380016977.01.T01:intron 35.0%
GAGTATGCAATGAAGATCAA+CGG - Chr3:90248398-90248417 MsG0380016977.01.T01:intron 35.0%
TCAAGGTCGAACAATAGTTT+AGG + Chr3:90246675-90246694 None:intergenic 35.0%
TGAGCATGCTTATCATGAAA+CGG - Chr3:90248370-90248389 MsG0380016977.01.T01:intron 35.0%
TGCCACCATTTCATTAATCA+TGG + Chr3:90248241-90248260 None:intergenic 35.0%
TTACTGCTTGTTTGACTGAA+GGG - Chr3:90248581-90248600 MsG0380016977.01.T01:CDS 35.0%
TTTCATTACAGGGGAACAAT+AGG + Chr3:90248169-90248188 None:intergenic 35.0%
! AAAGATGACTAGTCAATGCT+GGG - Chr3:90248260-90248279 MsG0380016977.01.T01:intron 35.0%
! AGATAAAGGGGTCTTCTTTT+TGG + Chr3:90247812-90247831 None:intergenic 35.0%
! AGCGGGTCTCAATTTTTAAT+GGG + Chr3:90247120-90247139 None:intergenic 35.0%
! TCCGATATATGCCACTTATA+TGG - Chr3:90248120-90248139 MsG0380016977.01.T01:intron 35.0%
! TGAGGCTATCAATTTTATGG+TGG - Chr3:90247575-90247594 MsG0380016977.01.T01:CDS 35.0%
! TGCTGAGGCTATCAATTTTA+TGG - Chr3:90247572-90247591 MsG0380016977.01.T01:CDS 35.0%
!! TTTGTTGTTCTTCCTGCATT+TGG + Chr3:90248215-90248234 None:intergenic 35.0%
AATCGACCAAGTCTAACCAA+CGG + Chr3:90247789-90247808 None:intergenic 40.0%
AGAATCAGACAACAGCATCA+TGG + Chr3:90246578-90246597 None:intergenic 40.0%
ATGGAATTGGTACCCAAACA+CGG - Chr3:90247893-90247912 MsG0380016977.01.T01:intron 40.0%
ATTATCTTCCCTCCGTGTTT+GGG + Chr3:90247908-90247927 None:intergenic 40.0%
CATTTCAGCAGAATTGAGGT+CGG + Chr3:90247308-90247327 None:intergenic 40.0%
CGGAATATGCCACATATAAG+TGG + Chr3:90248104-90248123 None:intergenic 40.0%
CTTACTGCTTGTTTGACTGA+AGG - Chr3:90248580-90248599 MsG0380016977.01.T01:CDS 40.0%
GATGCTGTTGTCTGATTCTT+TGG - Chr3:90246579-90246598 MsG0380016977.01.T01:exon 40.0%
GATTGCTTGGGTGTTAAACA+TGG + Chr3:90247095-90247114 None:intergenic 40.0%
GCATTTGGAGGGCAAAATTA+GGG + Chr3:90248200-90248219 None:intergenic 40.0%
TATGGCTTAGGGGATCTAAA+TGG - Chr3:90248064-90248083 MsG0380016977.01.T01:intron 40.0%
TATTATCTTCCCTCCGTGTT+TGG + Chr3:90247909-90247928 None:intergenic 40.0%
TCAATTCTGCTGAAATGCAG+AGG - Chr3:90247311-90247330 MsG0380016977.01.T01:CDS 40.0%
TCTGCATTTCAGCAGAATTG+AGG + Chr3:90247312-90247331 None:intergenic 40.0%
TGCATTTGGAGGGCAAAATT+AGG + Chr3:90248201-90248220 None:intergenic 40.0%
TGTGCTGTGTTCGTTCATTA+GGG - Chr3:90247212-90247231 MsG0380016977.01.T01:intron 40.0%
TTGTGCTGTGTTCGTTCATT+AGG - Chr3:90247211-90247230 MsG0380016977.01.T01:intron 40.0%
TTGTTCTTCCTGCATTTGGA+GGG + Chr3:90248211-90248230 None:intergenic 40.0%
! ACATTTCCGTTGGTTAGACT+TGG - Chr3:90247780-90247799 MsG0380016977.01.T01:intron 40.0%
! CAAAGATGACTAGTCAATGC+TGG - Chr3:90248259-90248278 MsG0380016977.01.T01:intron 40.0%
! CAGCGGGTCTCAATTTTTAA+TGG + Chr3:90247121-90247140 None:intergenic 40.0%
! GGATGTGAAGTGTTTATGCA+CGG - Chr3:90246614-90246633 MsG0380016977.01.T01:CDS 40.0%
! TAATTTTGCCCTCCAAATGC+AGG - Chr3:90248200-90248219 MsG0380016977.01.T01:intron 40.0%
! TGTTAGGCAATTAGTCTCAC+CGG + Chr3:90248325-90248344 None:intergenic 40.0%
!! AGAAAAGATGGCTTGTTCAG+CGG + Chr3:90247138-90247157 None:intergenic 40.0%
!! TTTTAATGGGCCGATTGCTT+GGG + Chr3:90247107-90247126 None:intergenic 40.0%
!! TTTTTAATGGGCCGATTGCT+TGG + Chr3:90247108-90247127 None:intergenic 40.0%
AAGAGAGACATGGCAAACAG+TGG + Chr3:90247944-90247963 None:intergenic 45.0%
AATTGGTACCCAAACACGGA+GGG - Chr3:90247897-90247916 MsG0380016977.01.T01:intron 45.0%
AGAGGAATGCAGAGAGAGAT+GGG - Chr3:90246547-90246566 MsG0380016977.01.T01:three_prime_UTR 45.0%
AGGGAGAGAAGTGAATCAGA+GGG - Chr3:90246402-90246421 MsG0380016977.01.T01:three_prime_UTR 45.0%
ATTGAGAGAGAGCGATTGAG+AGG + Chr3:90247277-90247296 None:intergenic 45.0%
CCTGGGAATAAGTTCAGTTC+CGG - Chr3:90248303-90248322 MsG0380016977.01.T01:intron 45.0%
GATGAAAAGCTCAGTGCATG+TGG - Chr3:90248550-90248569 MsG0380016977.01.T01:intron 45.0%
GTGTTTATGCACGGTGCATA+AGG - Chr3:90246623-90246642 MsG0380016977.01.T01:CDS 45.0%
GTTGTTCTTCCTGCATTTGG+AGG + Chr3:90248212-90248231 None:intergenic 45.0%
TCTCAATCGCTCTCTCTCAA+TGG - Chr3:90247276-90247295 MsG0380016977.01.T01:intron 45.0%
TTGAACCATATGTGGAGGGT+GGG + Chr3:90246905-90246924 None:intergenic 45.0%
TTTGAACCATATGTGGAGGG+TGG + Chr3:90246906-90246925 None:intergenic 45.0%
! ATTTGGTTCACTTCAGCTGC+TGG - Chr3:90247721-90247740 MsG0380016977.01.T01:intron 45.0%
!! GAAAAGATGGCTTGTTCAGC+GGG + Chr3:90247137-90247156 None:intergenic 45.0%
!!! AAGGCCAGTTGGTTTTTGGA+GGG - Chr3:90247663-90247682 MsG0380016977.01.T01:intron 45.0%
!!! TAAGGCCAGTTGGTTTTTGG+AGG - Chr3:90247662-90247681 MsG0380016977.01.T01:intron 45.0%
!!! TGCTAAGGCCAGTTGGTTTT+TGG - Chr3:90247659-90247678 MsG0380016977.01.T01:intron 45.0%
AACGCTGTGCACAGTGTGTT+AGG + Chr3:90248341-90248360 None:intergenic 50.0%
ACACTGTGCACAGCGTTACA+TGG - Chr3:90248344-90248363 MsG0380016977.01.T01:intron 50.0%
AGTCGTAGGACACGACGATT+AGG - Chr3:90247005-90247024 MsG0380016977.01.T01:intron 50.0%
ATTGAGAGGAGAGAGAGAGG+GGG + Chr3:90247263-90247282 None:intergenic 50.0%
CACAACCCACCTTAACCAGA+TGG + Chr3:90246322-90246341 None:intergenic 50.0%
CCGGAACTGAACTTATTCCC+AGG + Chr3:90248306-90248325 None:intergenic 50.0%
CGATTGAGAGGAGAGAGAGA+GGG + Chr3:90247265-90247284 None:intergenic 50.0%
CTGTTAGTGCTAAGGCCAGT+TGG - Chr3:90247652-90247671 MsG0380016977.01.T01:intron 50.0%
GAATTGGTACCCAAACACGG+AGG - Chr3:90247896-90247915 MsG0380016977.01.T01:intron 50.0%
GAGAGAAGTGAATCAGAGGG+AGG - Chr3:90246405-90246424 MsG0380016977.01.T01:three_prime_UTR 50.0%
GAGAGGAATGCAGAGAGAGA+TGG - Chr3:90246546-90246565 MsG0380016977.01.T01:three_prime_UTR 50.0%
GAGGAATGCAGAGAGAGATG+GGG - Chr3:90246548-90246567 MsG0380016977.01.T01:three_prime_UTR 50.0%
GAGGGAGAGAAGTGAATCAG+AGG - Chr3:90246401-90246420 MsG0380016977.01.T01:three_prime_UTR 50.0%
GATTGAGAGGAGAGAGAGAG+GGG + Chr3:90247264-90247283 None:intergenic 50.0%
GTAGGACACGACGATTAGGT+GGG - Chr3:90247009-90247028 MsG0380016977.01.T01:intron 50.0%
TGCTGGGACAAATGCATCAC+AGG - Chr3:90248276-90248295 MsG0380016977.01.T01:intron 50.0%
! ATGGTGTCAGGAGAGAATGG+CGG - Chr3:90246377-90246396 MsG0380016977.01.T01:three_prime_UTR 50.0%
! GAGATGGTGTCAGGAGAGAA+TGG - Chr3:90246374-90246393 MsG0380016977.01.T01:three_prime_UTR 50.0%
!! AGTTGATAGCGGTGGAGGAA+CGG - Chr3:90246466-90246485 MsG0380016977.01.T01:three_prime_UTR 50.0%
!!! AGGCCAGTTGGTTTTTGGAG+GGG - Chr3:90247664-90247683 MsG0380016977.01.T01:intron 50.0%
!!! CAGTTGGTTTTTGGAGGGGA+CGG - Chr3:90247668-90247687 MsG0380016977.01.T01:intron 50.0%
AAATGCATCACAGGCACGCC+TGG - Chr3:90248285-90248304 MsG0380016977.01.T01:intron 55.0%
AATGCATCACAGGCACGCCT+GGG - Chr3:90248286-90248305 MsG0380016977.01.T01:intron 55.0%
ACGCCGGCAAATGGTCTTTC+CGG - Chr3:90246345-90246364 MsG0380016977.01.T01:three_prime_UTR 55.0%
AGTGAATCAGAGGGAGGCAG+AGG - Chr3:90246411-90246430 MsG0380016977.01.T01:three_prime_UTR 55.0%
CGTAGGACACGACGATTAGG+TGG - Chr3:90247008-90247027 MsG0380016977.01.T01:intron 55.0%
CGTCCCCTCCAAAAACCAAC+TGG + Chr3:90247670-90247689 None:intergenic 55.0%
CTTCTCCCACCCTCCACATA+TGG - Chr3:90246897-90246916 MsG0380016977.01.T01:CDS 55.0%
GAATGCAGAGAGAGATGGGG+TGG - Chr3:90246551-90246570 MsG0380016977.01.T01:three_prime_UTR 55.0%
GCGATTGAGAGGAGAGAGAG+AGG + Chr3:90247266-90247285 None:intergenic 55.0%
GTTGTGAGAACGCCGGCAAA+TGG - Chr3:90246336-90246355 MsG0380016977.01.T01:three_prime_UTR 55.0%
!! AACGGAGTTGATAGCGGTGG+AGG - Chr3:90246461-90246480 MsG0380016977.01.T01:three_prime_UTR 55.0%
!! AAGGTGGGTTGTGAGAACGC+CGG - Chr3:90246329-90246348 MsG0380016977.01.T01:three_prime_UTR 55.0%
!! GTGGGGAACGGAGTTGATAG+CGG - Chr3:90246455-90246474 MsG0380016977.01.T01:three_prime_UTR 55.0%
!! GTTTTTGGAGGGGACGGCTA+AGG - Chr3:90247674-90247693 MsG0380016977.01.T01:intron 55.0%
AGGAACGGAAGTGACGGCGA+TGG - Chr3:90246481-90246500 MsG0380016977.01.T01:three_prime_UTR 60.0%
CCGCCGGAAAGACCATTTGC+CGG + Chr3:90246351-90246370 None:intergenic 60.0%
CCGGCAAATGGTCTTTCCGG+CGG - Chr3:90246348-90246367 MsG0380016977.01.T01:three_prime_UTR 60.0%
CGGTGGAGGAACGGAAGTGA+CGG - Chr3:90246475-90246494 MsG0380016977.01.T01:three_prime_UTR 60.0%
CTGACACCATCTCACTCCGC+CGG + Chr3:90246367-90246386 None:intergenic 60.0%
GAACGGAAGTGACGGCGATG+GGG - Chr3:90246483-90246502 MsG0380016977.01.T01:three_prime_UTR 60.0%
GAGGACAAGGACGCAGGTGA+CGG - Chr3:90246430-90246449 MsG0380016977.01.T01:three_prime_UTR 60.0%
GGAACGGAAGTGACGGCGAT+GGG - Chr3:90246482-90246501 MsG0380016977.01.T01:three_prime_UTR 60.0%
GTCTTTCCGGCGGAGTGAGA+TGG - Chr3:90246358-90246377 MsG0380016977.01.T01:three_prime_UTR 60.0%
TCAGAGGGAGGCAGAGGACA+AGG - Chr3:90246417-90246436 MsG0380016977.01.T01:three_prime_UTR 60.0%
TCAGGAGAGAATGGCGGCGA+GGG - Chr3:90246383-90246402 MsG0380016977.01.T01:three_prime_UTR 60.0%
TGGAGGTGGTCGGTTGAGAG+AGG - Chr3:90246529-90246548 MsG0380016977.01.T01:three_prime_UTR 60.0%
! ATGGGGTGGACGGATGATGG+AGG - Chr3:90246500-90246519 MsG0380016977.01.T01:three_prime_UTR 60.0%
!! ACGGATGATGGAGGGTGTGG+TGG - Chr3:90246509-90246528 MsG0380016977.01.T01:three_prime_UTR 60.0%
!! GATGATGGAGGGTGTGGTGG+AGG - Chr3:90246512-90246531 MsG0380016977.01.T01:three_prime_UTR 60.0%
!! GGGAACGGAGTTGATAGCGG+TGG - Chr3:90246458-90246477 MsG0380016977.01.T01:three_prime_UTR 60.0%
!! TGGACGGATGATGGAGGGTG+TGG - Chr3:90246506-90246525 MsG0380016977.01.T01:three_prime_UTR 60.0%
!! TGGGGTGGACGGATGATGGA+GGG - Chr3:90246501-90246520 MsG0380016977.01.T01:three_prime_UTR 60.0%
AGTGACGGCGATGGGGTGGA+CGG - Chr3:90246490-90246509 MsG0380016977.01.T01:three_prime_UTR 65.0%
CGGCGGAGTGAGATGGTGTC+AGG - Chr3:90246365-90246384 MsG0380016977.01.T01:three_prime_UTR 65.0%
GAGGCAGAGGACAAGGACGC+AGG - Chr3:90246424-90246443 MsG0380016977.01.T01:three_prime_UTR 65.0%
GCGATGGGGTGGACGGATGA+TGG - Chr3:90246497-90246516 MsG0380016977.01.T01:three_prime_UTR 65.0%
GTCAGGAGAGAATGGCGGCG+AGG - Chr3:90246382-90246401 MsG0380016977.01.T01:three_prime_UTR 65.0%
! GACAAGGACGCAGGTGACGG+CGG - Chr3:90246433-90246452 MsG0380016977.01.T01:three_prime_UTR 65.0%
! GAGGGTGTGGTGGAGGTGGT+CGG - Chr3:90246519-90246538 MsG0380016977.01.T01:three_prime_UTR 65.0%
! GATGGAGGGTGTGGTGGAGG+TGG - Chr3:90246515-90246534 MsG0380016977.01.T01:three_prime_UTR 65.0%
CGGAAGTGACGGCGATGGGG+TGG - Chr3:90246486-90246505 MsG0380016977.01.T01:three_prime_UTR 70.0%
! AAGGACGCAGGTGACGGCGG+TGG - Chr3:90246436-90246455 MsG0380016977.01.T01:three_prime_UTR 70.0%
! AGGACGCAGGTGACGGCGGT+GGG - Chr3:90246437-90246456 MsG0380016977.01.T01:three_prime_UTR 70.0%
! CAGGTGACGGCGGTGGGGAA+CGG - Chr3:90246443-90246462 MsG0380016977.01.T01:three_prime_UTR 70.0%
! GGACGCAGGTGACGGCGGTG+GGG - Chr3:90246438-90246457 MsG0380016977.01.T01:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 90246315 90248691 90246315 ID=MsG0380016977.01;Name=MsG0380016977.01
Chr3 mRNA 90246315 90248691 90246315 ID=MsG0380016977.01.T01;Parent=MsG0380016977.01;Name=MsG0380016977.01.T01;_AED=0.43;_eAED=0.65;_QI=0|0|0.2|0.8|1|1|5|281|203
Chr3 exon 90246315 90246784 90246315 ID=MsG0380016977.01.T01:exon:30833;Parent=MsG0380016977.01.T01
Chr3 exon 90246865 90246932 90246865 ID=MsG0380016977.01.T01:exon:30832;Parent=MsG0380016977.01.T01
Chr3 exon 90247289 90247447 90247289 ID=MsG0380016977.01.T01:exon:30831;Parent=MsG0380016977.01.T01
Chr3 exon 90247563 90247627 90247563 ID=MsG0380016977.01.T01:exon:30830;Parent=MsG0380016977.01.T01
Chr3 exon 90248561 90248691 90248561 ID=MsG0380016977.01.T01:exon:30829;Parent=MsG0380016977.01.T01
Chr3 CDS 90248561 90248691 90248561 ID=MsG0380016977.01.T01:cds;Parent=MsG0380016977.01.T01
Chr3 CDS 90247563 90247627 90247563 ID=MsG0380016977.01.T01:cds;Parent=MsG0380016977.01.T01
Chr3 CDS 90247289 90247447 90247289 ID=MsG0380016977.01.T01:cds;Parent=MsG0380016977.01.T01
Chr3 CDS 90246865 90246932 90246865 ID=MsG0380016977.01.T01:cds;Parent=MsG0380016977.01.T01
Chr3 CDS 90246596 90246784 90246596 ID=MsG0380016977.01.T01:cds;Parent=MsG0380016977.01.T01
Chr3 three_prime_UTR 90246315 90246595 90246315 ID=MsG0380016977.01.T01:three_prime_utr;Parent=MsG0380016977.01.T01
Gene Sequence

>MsG0380016977.01.T01

ATGGCCATCTGGTTAAGGTGGGTTGTGAGAACGCCGGCAAATGGTCTTTCCGGCGGAGTGAGATGGTGTCAGGAGAGAATGGCGGCGAGGGAGAGAAGTGAATCAGAGGGAGGCAGAGGACAAGGACGCAGATACTCCCTTTTTCAACTCGATTACATTTGTACTGTTTTTGCTTTGTTACTTGTTCATCAATTCAATTTGATTTTTCATGCTGAGGCTATCAATTTTATGGTGGCTAAGGTTGATTCTGTTACTATAGTTTTAAAATTGCAAAACAACACATACTGTTCTGTTAGTGCTAAGGCCAGTTGGTTTTTGGAGGGGACGGCTAAGGATATGTATTTGATATTTAAAAGGGATCTAAATGGTATTCCGATATATACCACTTATATGTGGCATATTCCGATATATGCCACTTATATGGAAGAACAACAAAGAGCCATGATTAATGAAATGGTGGCAAAGATGACTAGTCAATGCTGGGACAAATGCATCACAGGCACGCCTGGGAATAAGTTCAGTTCCGGTGAGACTAATTGCCTAACACACTGTGCACAGCGTTACATGGAGATGAGCATGCTTATCATGAAACGGTTTCAGAGTATGCAATGA
Protein sequence

>MsG0380016977.01.T01

MAIWLRWVVRTPANGLSGGVRWCQERMAARERSESEGGRGQGRRYSLFQLDYICTVFALLLVHQFNLIFHAEAINFMVAKVDSVTIVLKLQNNTYCSVSAKASWFLEGTAKDMYLIFKRDLNGIPIYTTYMWHIPIYATYMEEQQRAMINEMVAKMTSQCWDKCITGTPGNKFSSGETNCLTHCAQRYMEMSMLIMKRFQSMQ*