AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023962.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023962.01.T01 MTR_8g105890 99.010 101 1 0 35 135 8 108 6.12e-70 206
MsG0480023962.01.T01 MTR_8g105890 100.000 100 0 0 36 135 25 124 8.43e-70 206
MsG0480023962.01.T01 MTR_5g097030 92.079 101 8 0 35 135 59 159 1.41e-62 189
MsG0480023962.01.T01 MTR_8g105890 100.000 82 0 0 54 135 1 82 9.34e-56 169
MsG0480023962.01.T03 MTR_8g105890 100.000 108 0 0 1 108 1 108 2.29e-76 221
MsG0480023962.01.T03 MTR_8g105890 100.000 100 0 0 9 108 25 124 6.54e-70 205
MsG0480023962.01.T03 MTR_5g097030 91.509 106 8 1 3 108 55 159 4.71e-65 194
MsG0480023962.01.T03 MTR_8g105890 100.000 82 0 0 27 108 1 82 6.03e-56 168
MsG0480023962.01.T02 MTR_8g105890 91.089 101 1 1 35 127 8 108 6.44e-61 182
MsG0480023962.01.T02 MTR_8g105890 92.000 100 0 1 36 127 25 124 1.18e-60 182
MsG0480023962.01.T02 MTR_5g097030 84.158 101 8 1 35 127 59 159 3.23e-53 165
MsG0480023962.01.T02 MTR_8g105890 90.244 82 0 1 54 127 1 82 1.13e-46 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023962.01.T01 AT3G62840 95.918 98 4 0 38 135 12 109 1.11e-65 195
MsG0480023962.01.T01 AT2G47640 95.918 98 4 0 38 135 12 109 1.11e-65 195
MsG0480023962.01.T01 AT2G47640 95.918 98 4 0 38 135 12 109 1.11e-65 195
MsG0480023962.01.T01 AT3G62840 95.918 98 4 0 38 135 11 108 1.53e-65 194
MsG0480023962.01.T01 AT2G47640 95.918 98 4 0 38 135 11 108 1.53e-65 194
MsG0480023962.01.T01 AT2G47640 95.918 98 4 0 38 135 11 108 1.53e-65 194
MsG0480023962.01.T03 AT3G62840 93.519 108 7 0 1 108 1 108 3.57e-71 207
MsG0480023962.01.T03 AT2G47640 93.519 108 7 0 1 108 1 108 3.57e-71 207
MsG0480023962.01.T03 AT2G47640 93.519 108 7 0 1 108 1 108 3.57e-71 207
MsG0480023962.01.T03 AT3G62840 92.661 109 7 1 1 108 1 109 1.41e-69 203
MsG0480023962.01.T03 AT2G47640 92.661 109 7 1 1 108 1 109 1.41e-69 203
MsG0480023962.01.T03 AT2G47640 92.661 109 7 1 1 108 1 109 1.41e-69 203
MsG0480023962.01.T02 AT3G62840 87.755 98 4 1 38 127 12 109 1.52e-56 171
MsG0480023962.01.T02 AT2G47640 87.755 98 4 1 38 127 12 109 1.52e-56 171
MsG0480023962.01.T02 AT2G47640 87.755 98 4 1 38 127 12 109 1.52e-56 171
MsG0480023962.01.T02 AT3G62840 87.755 98 4 1 38 127 11 108 1.66e-56 171
MsG0480023962.01.T02 AT2G47640 87.755 98 4 1 38 127 11 108 1.66e-56 171
MsG0480023962.01.T02 AT2G47640 87.755 98 4 1 38 127 11 108 1.66e-56 171

Find 17 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGAGATACTAGCTTATT+TGG 0.120204 4:+91724889 None:intergenic
ATTCTGTCATCATTGTTCTT+AGG 0.279548 4:-91724916 MsG0480023962.01.T01:CDS
ATGGAGATACTAGCTTATTT+GGG 0.292207 4:+91724890 None:intergenic
ATCAGCAAAATGTTCCTTCG+TGG 0.434985 4:-91724941 MsG0480023962.01.T01:CDS
TAGCTTACATCTTCTTCCAT+TGG 0.480536 4:+91726037 None:intergenic
TTACATCTTCTTCCATTGGC+CGG 0.497216 4:+91726041 None:intergenic
ACTCATTGTTACAGGCTGTC+GGG 0.503568 4:-91725392 MsG0480023962.01.T01:CDS
CTCATCATGAGTACAGATAG+TGG 0.509132 4:+91725339 None:intergenic
GAAGGCTCAGCCAGTCAACA+AGG 0.537512 4:-91724972 MsG0480023962.01.T01:CDS
ATGAAACGATCCTTGTTGAC+TGG 0.541044 4:+91724962 None:intergenic
TTCTTCTTGAAGCCGGCCAA+TGG 0.551208 4:-91726053 MsG0480023962.01.T03:intron
TGTCGGGAAGAATGAGGAAG+AGG 0.555164 4:-91725376 MsG0480023962.01.T01:CDS
TACTCATTGTTACAGGCTGT+CGG 0.558965 4:-91725393 MsG0480023962.01.T01:CDS
TGGAGATACTAGCTTATTTG+GGG 0.598626 4:+91724891 None:intergenic
ACAGGCTGTCGGGAAGAATG+AGG 0.617342 4:-91725382 MsG0480023962.01.T01:CDS
GAGGAAGAGGAATTCAACAC+TGG 0.639973 4:-91725363 MsG0480023962.01.T01:CDS
TGATGACAGAATCTCCACGA+AGG 0.746510 4:+91724927 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAAAAAAAAAAAAA+TGG + Chr4:91725203-91725222 None:intergenic 0.0%
!!! TTTTTTTTTTTAAAAAACAG+AGG - Chr4:91725206-91725225 MsG0480023962.01.T01:CDS 10.0%
!! AAGATTGAGCTTAAATAATA+AGG + Chr4:91726146-91726165 None:intergenic 20.0%
!!! ACTTTGACTTTAATGTTAAA+TGG - Chr4:91725284-91725303 MsG0480023962.01.T01:intron 20.0%
!!! TGTTTTATCATTACATAACT+TGG - Chr4:91725006-91725025 MsG0480023962.01.T01:intron 20.0%
! AACAAGATCAAACAAATTTG+CGG - Chr4:91726019-91726038 MsG0480023962.01.T03:intron 25.0%
! ATCATTACATAACTTGGTAA+AGG - Chr4:91725012-91725031 MsG0480023962.01.T01:intron 25.0%
! TGCATTATTGGTATGAAATA+TGG - Chr4:91725920-91725939 MsG0480023962.01.T03:intron 25.0%
! TTGAGCTTAAATAATAAGGA+AGG + Chr4:91726142-91726161 None:intergenic 25.0%
!! TCAATATGCTCTATTTGTTT+TGG - Chr4:91724951-91724970 MsG0480023962.01.T01:CDS 25.0%
ATATGGTTCTTGAAAATGTC+CGG - Chr4:91725606-91725625 MsG0480023962.01.T03:intron 30.0%
ATGCAAATTACTTCTCTACT+TGG - Chr4:91725662-91725681 MsG0480023962.01.T03:intron 30.0%
ATTCTGTCATCATTGTTCTT+AGG - Chr4:91725827-91725846 MsG0480023962.01.T03:intron 30.0%
CATTTGAATTTACTTTCTGC+TGG - Chr4:91725687-91725706 MsG0480023962.01.T03:intron 30.0%
CTCCACGTTTCAATAAAATT+TGG + Chr4:91725959-91725978 None:intergenic 30.0%
GAAGAAAGAAAAAAGAAGAC+TGG + Chr4:91725240-91725259 None:intergenic 30.0%
TGAAATCACAATTCTGAATC+AGG + Chr4:91724977-91724996 None:intergenic 30.0%
TGTAGCTTCTGTTAAAATCA+AGG + Chr4:91724858-91724877 None:intergenic 30.0%
TGTTCTATTTAGTGCACATT+TGG - Chr4:91724799-91724818 MsG0480023962.01.T01:three_prime_UTR 30.0%
TTATTGAAACGTGGAGAAAT+TGG - Chr4:91725963-91725982 MsG0480023962.01.T03:intron 30.0%
TTTGCATTATGATCCTGATA+TGG - Chr4:91724761-91724780 MsG0480023962.01.T01:three_prime_UTR 30.0%
! AGCCAAATTTTATTGAAACG+TGG - Chr4:91725954-91725973 MsG0480023962.01.T03:intron 30.0%
! ATGGAGATACTAGCTTATTT+GGG + Chr4:91725856-91725875 None:intergenic 30.0%
! GATTTTACAAACACTTATGC+AGG - Chr4:91725517-91725536 MsG0480023962.01.T03:intron 30.0%
! TGTAGTGATGATGTATGAAA+TGG - Chr4:91726064-91726083 MsG0480023962.01.T03:intron 30.0%
! TTGATTTTTCTTCTTGAAGC+CGG - Chr4:91724683-91724702 MsG0480023962.01.T01:three_prime_UTR 30.0%
! TTTTGATAGACACTGCAATA+TGG - Chr4:91725589-91725608 MsG0480023962.01.T03:intron 30.0%
!! ATTTTAACAGAAGCTACACT+TGG - Chr4:91724860-91724879 MsG0480023962.01.T01:three_prime_UTR 30.0%
!!! GCTTTGTTTTCTTGCATTAT+TGG - Chr4:91725908-91725927 MsG0480023962.01.T03:intron 30.0%
AAAAAAGAAGACTGGACTTC+AGG + Chr4:91725232-91725251 None:intergenic 35.0%
ACAGATATGCAATCCATATC+AGG + Chr4:91724777-91724796 None:intergenic 35.0%
ATGTTCTGCTTGTATGTATC+AGG - Chr4:91725723-91725742 MsG0480023962.01.T03:intron 35.0%
ATTTGGATTGACCTTGAGTT+TGG - Chr4:91724816-91724835 MsG0480023962.01.T01:three_prime_UTR 35.0%
CGTAACAACAAAAAGCTTCT+AGG - Chr4:91725554-91725573 MsG0480023962.01.T03:intron 35.0%
TACTGTGTGTTCTTCTTGAT+CGG - Chr4:91725473-91725492 MsG0480023962.01.T01:CDS 35.0%
TAGCTTACATCTTCTTCCAT+TGG + Chr4:91724709-91724728 None:intergenic 35.0%
TATGGTTCTTGAAAATGTCC+GGG - Chr4:91725607-91725626 MsG0480023962.01.T03:intron 35.0%
TGAGTTTGACTAAATCATGC+TGG + Chr4:91724907-91724926 None:intergenic 35.0%
TTCTTTCCTTTACCAGTCTT+AGG + Chr4:91725751-91725770 None:intergenic 35.0%
TTGAATTTACTTTCTGCTGG+TGG - Chr4:91725690-91725709 MsG0480023962.01.T03:intron 35.0%
! GATGGAGATACTAGCTTATT+TGG + Chr4:91725857-91725876 None:intergenic 35.0%
! TGGAGATACTAGCTTATTTG+GGG + Chr4:91725855-91725874 None:intergenic 35.0%
!!! AAGTAGTGGGCTCTTATTTT+TGG - Chr4:91725886-91725905 MsG0480023962.01.T03:intron 35.0%
ATCAGCAAAATGTTCCTTCG+TGG - Chr4:91725802-91725821 MsG0480023962.01.T03:intron 40.0%
ATGAAACGATCCTTGTTGAC+TGG + Chr4:91725784-91725803 None:intergenic 40.0%
CTCATCATGAGTACAGATAG+TGG + Chr4:91725407-91725426 None:intergenic 40.0%
GACTGGTAAAGGAAAGAAGA+AGG - Chr4:91725753-91725772 MsG0480023962.01.T03:intron 40.0%
GAGTGTGAAAAACAACACAC+AGG - Chr4:91725424-91725443 MsG0480023962.01.T01:CDS 40.0%
GCTGACATACTCATTGTTAC+AGG - Chr4:91725343-91725362 MsG0480023962.01.T01:CDS 40.0%
TACTCATTGTTACAGGCTGT+CGG - Chr4:91725350-91725369 MsG0480023962.01.T01:CDS 40.0%
TCTTTCTTCATGCGTCACAA+TGG - Chr4:91725251-91725270 MsG0480023962.01.T01:intron 40.0%
TGAGGTATGTATTCTCTTGC+TGG - Chr4:91724591-91724610 MsG0480023962.01.T01:three_prime_UTR 40.0%
TGTATGAAATGGTTCGACTC+TGG - Chr4:91726075-91726094 MsG0480023962.01.T03:intron 40.0%
TTACATCTTCTTCCATTGGC+CGG + Chr4:91724705-91724724 None:intergenic 40.0%
TTGAAAATGTCCGGGAAATG+TGG - Chr4:91725615-91725634 MsG0480023962.01.T03:intron 40.0%
TTGAAACGTGGAGAAATTGG+TGG - Chr4:91725966-91725985 MsG0480023962.01.T03:intron 40.0%
TTGGTGGAGGATCTTGATTT+AGG - Chr4:91725982-91726001 MsG0480023962.01.T03:intron 40.0%
! TGAAAGTCAGCTTTGTGTTG+TGG - Chr4:91725099-91725118 MsG0480023962.01.T01:intron 40.0%
AAACGTGGAGAAATTGGTGG+AGG - Chr4:91725969-91725988 MsG0480023962.01.T03:intron 45.0%
ACTCATTGTTACAGGCTGTC+GGG - Chr4:91725351-91725370 MsG0480023962.01.T01:CDS 45.0%
ATACCTCAGTCCACATTTCC+CGG + Chr4:91725628-91725647 None:intergenic 45.0%
ATGTATCAGGTGCCTAAGAC+TGG - Chr4:91725736-91725755 MsG0480023962.01.T03:intron 45.0%
CATCCCTCTGTTGAAGTAGT+GGG - Chr4:91725873-91725892 MsG0480023962.01.T03:intron 45.0%
GAGGAAGAGGAATTCAACAC+TGG - Chr4:91725380-91725399 MsG0480023962.01.T01:CDS 45.0%
GTCTATCAAAAGCCCTGACA+CGG + Chr4:91725580-91725599 None:intergenic 45.0%
TGATGACAGAATCTCCACGA+AGG + Chr4:91725819-91725838 None:intergenic 45.0%
TGGATTGACCTTGAGTTTGG+CGG - Chr4:91724819-91724838 MsG0480023962.01.T01:three_prime_UTR 45.0%
AAAAGCTTCTAGGCCGTGTC+AGG - Chr4:91725564-91725583 MsG0480023962.01.T03:intron 50.0%
AAAGCTTCTAGGCCGTGTCA+GGG - Chr4:91725565-91725584 MsG0480023962.01.T03:intron 50.0%
AGAGCCCACTACTTCAACAG+AGG + Chr4:91725880-91725899 None:intergenic 50.0%
CAGGTGCCTAAGACTGGTAA+AGG - Chr4:91725742-91725761 MsG0480023962.01.T03:intron 50.0%
CCAAGATTCCGCCAAACTCA+AGG + Chr4:91724830-91724849 None:intergenic 50.0%
CCACTACTTCAACAGAGGGA+TGG + Chr4:91725875-91725894 None:intergenic 50.0%
CCATCCCTCTGTTGAAGTAG+TGG - Chr4:91725872-91725891 MsG0480023962.01.T03:intron 50.0%
CCTTGAGTTTGGCGGAATCT+TGG - Chr4:91724827-91724846 MsG0480023962.01.T01:three_prime_UTR 50.0%
GAGCCCACTACTTCAACAGA+GGG + Chr4:91725879-91725898 None:intergenic 50.0%
TGTCGGGAAGAATGAGGAAG+AGG - Chr4:91725367-91725386 MsG0480023962.01.T01:CDS 50.0%
TTCTTCTTGAAGCCGGCCAA+TGG - Chr4:91724690-91724709 MsG0480023962.01.T01:three_prime_UTR 50.0%
ACAGGCTGTCGGGAAGAATG+AGG - Chr4:91725361-91725380 MsG0480023962.01.T01:CDS 55.0%
GAAGGCTCAGCCAGTCAACA+AGG - Chr4:91725771-91725790 MsG0480023962.01.T03:intron 55.0%
! TGTCCGGGAAATGTGGACTG+AGG - Chr4:91725622-91725641 MsG0480023962.01.T03:intron 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 91724590 91726175 91724590 ID=MsG0480023962.01;Name=MsG0480023962.01
Chr4 mRNA 91724590 91725505 91724590 ID=MsG0480023962.01.T01;Parent=MsG0480023962.01;Name=MsG0480023962.01.T01;_AED=0.43;_eAED=0.43;_QI=0|1|1|1|0.4|0.33|6|314|135
Chr4 CDS 91725320 91725505 91725320 ID=MsG0480023962.01.T01:cds;Parent=MsG0480023962.01.T01
Chr4 CDS 91725122 91725226 91725122 ID=MsG0480023962.01.T01:cds;Parent=MsG0480023962.01.T01
Chr4 CDS 91724904 91725020 91724904 ID=MsG0480023962.01.T01:cds;Parent=MsG0480023962.01.T01
Chr4 three_prime_UTR 91724590 91724903 91724590 ID=MsG0480023962.01.T01:three_prime_utr;Parent=MsG0480023962.01.T01
Chr4 mRNA 91724904 91725505 91724904 ID=MsG0480023962.01.T02;Parent=MsG0480023962.01;Name=MsG0480023962.01.T02;_AED=0.45;_eAED=0.45;_QI=0|0.8|0.83|1|0.2|0.16|6|0|127
Chr4 exon 91724904 91724996 91724904 ID=MsG0480023962.01.T02:exon:8002;Parent=MsG0480023962.01.T02
Chr4 CDS 91725320 91725505 91725320 ID=MsG0480023962.01.T02:cds;Parent=MsG0480023962.01.T02
Chr4 CDS 91725122 91725226 91725122 ID=MsG0480023962.01.T02:cds;Parent=MsG0480023962.01.T02
Chr4 CDS 91724904 91724996 91724904 ID=MsG0480023962.01.T02:cds;Parent=MsG0480023962.01.T02
Chr4 mRNA 91724590 91726175 91724590 ID=MsG0480023962.01.T03;Parent=MsG0480023962.01;Name=MsG0480023962.01.T03;_AED=0.46;_eAED=0.46;_QI=0|1|1|1|0.4|0.5|6|314|108
Chr4 exon 91725320 91725400 91725320 ID=MsG0480023962.01.T03:exon:8005;Parent=MsG0480023962.01.T03
Chr4 exon 91726045 91726063 91726045 ID=MsG0480023962.01.T03:exon:8004;Parent=MsG0480023962.01.T03
Chr4 exon 91726171 91726175 91726171 ID=MsG0480023962.01.T03:exon:8003;Parent=MsG0480023962.01.T03
Chr4 CDS 91726171 91726175 91726171 ID=MsG0480023962.01.T03:cds;Parent=MsG0480023962.01.T03
Chr4 CDS 91726045 91726063 91726045 ID=MsG0480023962.01.T03:cds;Parent=MsG0480023962.01.T03
Chr4 CDS 91725320 91725400 91725320 ID=MsG0480023962.01.T03:cds;Parent=MsG0480023962.01.T03
Chr4 CDS 91725122 91725226 91725122 ID=MsG0480023962.01.T03:cds;Parent=MsG0480023962.01.T03
Chr4 CDS 91724904 91725020 91724904 ID=MsG0480023962.01.T03:cds;Parent=MsG0480023962.01.T03
Chr4 three_prime_UTR 91724590 91724903 91724590 ID=MsG0480023962.01.T03:three_prime_utr;Parent=MsG0480023962.01.T03
Gene Sequence

>MsG0480023962.01.T01

ATGCGTCACAATGGTTCTTACTGCACTTTGACTTTAATGTTAAATGGCTGTTTAAAGTTTAATATGCTTTATAATCTTGTCAAGCTGACATACTCATTGTTACAGGCTGTCGGGAAGAATGAGGAAGAGGAATTCAACACTGGTCCACTATCTGTACTCATGATGAGTGTGAAAAACAACACACAGGTTCTGATCAATTGTCGTAACAACAAAAAGCTTCTAGGCCGTGTCAGGGCTTTTGATAGACACTGCAATATGGTTCTTGAAAATGTCCGGGAAATGTGGACTGAGGTGCCTAAGACTGGTAAAGGAAAGAAGAAGGCTCAGCCAGTCAACAAGGATCGTTTCATCAGCAAAATGTTCCTTCGTGGAGATTCTGTCATCATTGTTCTTAGGAACCCCAAATAA

>MsG0480023962.01.T03

ATGAGCCGGCCAATGGAAGAAGATGCTGTCGGGAAGAATGAGGAAGAGGAATTCAACACTGGTCCACTATCTGTACTCATGATGAGTGTGAAAAACAACACACAGGTTCTGATCAATTGTCGTAACAACAAAAAGCTTCTAGGCCGTGTCAGGGCTTTTGATAGACACTGCAATATGGTTCTTGAAAATGTCCGGGAAATGTGGACTGAGGTGCCTAAGACTGGTAAAGGAAAGAAGAAGGCTCAGCCAGTCAACAAGGATCGTTTCATCAGCAAAATGTTCCTTCGTGGAGATTCTGTCATCATTGTTCTTAGGAACCCCAAATAA

>MsG0480023962.01.T02

ATGCGTCACAATGGTTCTTACTGCACTTTGACTTTAATGTTAAATGGCTGTTTAAAGTTTAATATGCTTTATAATCTTGTCAAGCTGACATACTCATTGTTACAGGCTGTCGGGAAGAATGAGGAAGAGGAATTCAACACTGGTCCACTATCTGTACTCATGATGAGTGTGAAAAACAACACACAGGTTCTGATCAATTGTCGTAACAACAAAAAGCTTCTAGGCCGTGTCAGGGCTTTTGATAGACACTGCAATATGGTTCTTGAAAATGTCCGGGAAATGTGGACTGAGAAGAAGGCTCAGCCAGTCAACAAGGATCGTTTCATCAGCAAAATGTTCCTTCGTGGAGATTCTGTCATCATTGTTCTTAGGAACCCCAAATAA

Protein sequence

>MsG0480023962.01.T01

MRHNGSYCTLTLMLNGCLKFNMLYNLVKLTYSLLQAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKAQPVNKDRFISKMFLRGDSVIIVLRNPK*

>MsG0480023962.01.T03

MSRPMEEDAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKAQPVNKDRFISKMFLRGDSVIIVLRNPK*

>MsG0480023962.01.T02

MRHNGSYCTLTLMLNGCLKFNMLYNLVKLTYSLLQAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEKKAQPVNKDRFISKMFLRGDSVIIVLRNPK*