Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017682.01.T01 | XP_013456998.1 | 75.641 | 78 | 11 | 2 | 1 | 70 | 307 | 384 | 3.31E-22 | 98.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017682.01.T01 | A0A072UML9 | 75.641 | 78 | 11 | 2 | 1 | 70 | 307 | 384 | 1.58e-22 | 98.2 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048515.01 | MsG0380017682.01 | 0.800933 | 1.142092e-48 | 5.385358e-46 |
| MsG0180000470.01 | MsG0380017682.01 | 0.810919 | 9.072844e-51 | 5.540039e-48 |
| MsG0380017682.01 | MsG0480018653.01 | 0.800868 | 1.177903e-48 | 5.544877e-46 |
| MsG0380017682.01 | MsG0680030880.01 | 0.812466 | 4.179719e-51 | 2.659710e-48 |
| MsG0380017682.01 | MsG0780037457.01 | 0.802596 | 5.204612e-49 | 2.559786e-46 |
| MsG0280007072.01 | MsG0380017682.01 | 0.804358 | 2.244341e-49 | 1.154827e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017682.01.T01 | MTR_4g088580 | 75.641 | 78 | 11 | 2 | 1 | 70 | 307 | 384 | 4.01e-26 | 98.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATTCTTCTTCTCTTTAT+TGG | 0.115189 | 3:+99515405 | MsG0380017682.01.T01:CDS |
| TGAAAACCAGATGGAGAATT+TGG | 0.129395 | 3:+99515376 | MsG0380017682.01.T01:CDS |
| GAATTATGACCAATATTATT+AGG | 0.234994 | 3:-99515500 | None:intergenic |
| CAACAAGTTTGAGTAGTGTT+TGG | 0.345092 | 3:+99515462 | MsG0380017682.01.T01:CDS |
| GAAAACCAGATGGAGAATTT+GGG | 0.394132 | 3:+99515377 | MsG0380017682.01.T01:CDS |
| GATCATCAACCTAATAATAT+TGG | 0.412223 | 3:+99515491 | MsG0380017682.01.T01:CDS |
| TTCAGTTACTTCTCTGATCT+AGG | 0.441864 | 3:+99515520 | MsG0380017682.01.T01:CDS |
| ACAAACCCAAATTCTCCATC+TGG | 0.460332 | 3:-99515382 | None:intergenic |
| AGTAATGATATGAATGCTAA+TGG | 0.543549 | 3:+99515341 | MsG0380017682.01.T01:CDS |
| TACAAAATAGGATGGATCAG+TGG | 0.666710 | 3:+99515318 | None:intergenic |
| AATGATATGAATGCTAATGG+TGG | 0.699787 | 3:+99515344 | MsG0380017682.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAATTATGACCAATATTATT+AGG | - | Chr3:99515503-99515522 | None:intergenic | 20.0% |
| ! | AGTAATGATATGAATGCTAA+TGG | + | Chr3:99515341-99515360 | MsG0380017682.01.T01:CDS | 25.0% |
| ! | GATCATCAACCTAATAATAT+TGG | + | Chr3:99515491-99515510 | MsG0380017682.01.T01:CDS | 25.0% |
| AATGATATGAATGCTAATGG+TGG | + | Chr3:99515344-99515363 | MsG0380017682.01.T01:CDS | 30.0% | |
| CAGATTCTTCTTCTCTTTAT+TGG | + | Chr3:99515405-99515424 | MsG0380017682.01.T01:CDS | 30.0% | |
| CAACAAGTTTGAGTAGTGTT+TGG | + | Chr3:99515462-99515481 | MsG0380017682.01.T01:CDS | 35.0% | |
| GAAAACCAGATGGAGAATTT+GGG | + | Chr3:99515377-99515396 | MsG0380017682.01.T01:CDS | 35.0% | |
| TGAAAACCAGATGGAGAATT+TGG | + | Chr3:99515376-99515395 | MsG0380017682.01.T01:CDS | 35.0% | |
| ACAAACCCAAATTCTCCATC+TGG | - | Chr3:99515385-99515404 | None:intergenic | 40.0% | |
| ! | TGCAGTGTTGTTGTTGTTGT+TGG | - | Chr3:99515441-99515460 | None:intergenic | 40.0% |
| !!! | CGTTGCTTTTGAAAACCAGA+TGG | + | Chr3:99515367-99515386 | MsG0380017682.01.T01:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 99515329 | 99515541 | 99515329 | ID=MsG0380017682.01;Name=MsG0380017682.01 |
| Chr3 | mRNA | 99515329 | 99515541 | 99515329 | ID=MsG0380017682.01.T01;Parent=MsG0380017682.01;Name=MsG0380017682.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|70 |
| Chr3 | exon | 99515329 | 99515541 | 99515329 | ID=MsG0380017682.01.T01:exon:2663;Parent=MsG0380017682.01.T01 |
| Chr3 | CDS | 99515329 | 99515541 | 99515329 | ID=MsG0380017682.01.T01:cds;Parent=MsG0380017682.01.T01 |
| Gene Sequence |
| Protein sequence |
