Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017789.01.T01 | KEH36177.1 | 86.806 | 288 | 27 | 2 | 1 | 288 | 1 | 277 | 3.66E-169 | 479 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017789.01.T01 | A0A072V2U5 | 86.806 | 288 | 27 | 2 | 1 | 288 | 1 | 277 | 1.75e-169 | 479 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011411.01 | MsG0380017789.01 | 0.800425 | 1.449767e-48 | 6.748633e-46 |
| MsG0380011495.01 | MsG0380017789.01 | 0.804458 | 2.139216e-49 | 1.103554e-46 |
| MsG0380017728.01 | MsG0380017789.01 | 0.812422 | 4.274220e-51 | 2.716605e-48 |
| MsG0380017789.01 | MsG0480018324.01 | 0.813896 | 2.029040e-51 | 1.341638e-48 |
| MsG0380017789.01 | MsG0780041092.01 | 0.815134 | 1.080156e-51 | 7.384779e-49 |
| MsG0380017789.01 | MsG0880047111.01 | 0.800183 | 1.623876e-48 | 7.512422e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017789.01.T01 | MTR_3g112100 | 86.806 | 288 | 27 | 2 | 1 | 288 | 1 | 277 | 4.44e-173 | 479 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 62 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAAATTAATTTCCTAATA+TGG | 0.169496 | 3:-100674685 | None:intergenic |
| TGAATCTCCTCAACTCTTTC+GGG | 0.202962 | 3:-100674495 | None:intergenic |
| TTGAATCTCCTCAACTCTTT+CGG | 0.229356 | 3:-100674496 | None:intergenic |
| CTTGGCCCTGACTCCATATT+AGG | 0.296205 | 3:+100674672 | MsG0380017789.01.T01:CDS |
| GACTCTACGCGATCGGTAAC+AGG | 0.314731 | 3:-100674229 | None:intergenic |
| TGGTGGAAACGTCGTCGTTT+TGG | 0.336274 | 3:-100674045 | None:intergenic |
| GACAACTACGGGAGATAATA+AGG | 0.353674 | 3:+100674405 | MsG0380017789.01.T01:CDS |
| TTGGTTGGAGAGTTTATATA+TGG | 0.358818 | 3:-100674026 | None:intergenic |
| TTTCGGAGGAAAAGTGTTAC+AGG | 0.363849 | 3:+100674428 | MsG0380017789.01.T01:CDS |
| GGATCTTGTTGTTGTTTCTC+AGG | 0.379351 | 3:-100674175 | None:intergenic |
| TAATTTCCTAATATGGAGTC+AGG | 0.394555 | 3:-100674678 | None:intergenic |
| AGAGTCGCTGAAGAGGGATT+TGG | 0.407242 | 3:-100673966 | None:intergenic |
| TAAAATAACCGCAAAGGAAC+CGG | 0.410022 | 3:+100674141 | MsG0380017789.01.T01:CDS |
| TACGGGAGATAATAAGGTTT+CGG | 0.411849 | 3:+100674411 | MsG0380017789.01.T01:CDS |
| AAAGCAAGAGAGTGGCAAAA+AGG | 0.415088 | 3:-100673883 | None:intergenic |
| GTAACTGCCATGACAGGTTC+AGG | 0.423281 | 3:-100674334 | None:intergenic |
| TTACCGATCGCGTAGAGTCT+TGG | 0.447245 | 3:+100674233 | MsG0380017789.01.T01:CDS |
| CAACACATGTTTGAAGAAGA+AGG | 0.449031 | 3:+100674516 | MsG0380017789.01.T01:CDS |
| ACAAGTGGAAAATGTATTCA+AGG | 0.458274 | 3:-100673856 | None:intergenic |
| TGTTGAAGAATTGAGTAAAG+AGG | 0.462598 | 3:+100674561 | MsG0380017789.01.T01:CDS |
| GTCTCAGAAAACAGAGTACT+TGG | 0.484229 | 3:+100674654 | MsG0380017789.01.T01:CDS |
| GGAGTGTTATGAACGGAGTC+TGG | 0.486988 | 3:+100674462 | MsG0380017789.01.T01:CDS |
| AACAAACAAACACCATGAAA+AGG | 0.490054 | 3:+100673813 | None:intergenic |
| ACATAAGAAGATGATGCAAT+GGG | 0.497256 | 3:+100674618 | MsG0380017789.01.T01:CDS |
| AGACTCTACCGGTTCCTTTG+CGG | 0.506127 | 3:-100674149 | None:intergenic |
| TCTTAAAATAACCTCTTCAT+CGG | 0.520835 | 3:+100674084 | MsG0380017789.01.T01:CDS |
| GTGTTACAGGAGAGTTCAAT+CGG | 0.523641 | 3:+100674441 | MsG0380017789.01.T01:CDS |
| AACATAAGAAGATGATGCAA+TGG | 0.524063 | 3:+100674617 | MsG0380017789.01.T01:CDS |
| AAGAGCGTAGAGATGTGAAA+AGG | 0.528813 | 3:-100673942 | None:intergenic |
| GTCTCAGTAACTGCCATGAC+AGG | 0.529041 | 3:-100674340 | None:intergenic |
| TTCTCAGGCAAAGACTCTAC+CGG | 0.530771 | 3:-100674160 | None:intergenic |
| TTGCACGACCGTGACAACTA+CGG | 0.535525 | 3:+100674393 | MsG0380017789.01.T01:CDS |
| TGCACGACCGTGACAACTAC+GGG | 0.538337 | 3:+100674394 | MsG0380017789.01.T01:CDS |
| CATAGAAAGAGTCGCTGAAG+AGG | 0.548724 | 3:-100673973 | None:intergenic |
| TCGCGTAGAGTCTTGGTTGG+AGG | 0.551551 | 3:+100674240 | MsG0380017789.01.T01:CDS |
| TTCTCCAACGAAGACACTAC+AGG | 0.555602 | 3:-100674196 | None:intergenic |
| AGATCCTGTAGTGTCTTCGT+TGG | 0.558492 | 3:+100674192 | MsG0380017789.01.T01:CDS |
| AAAACATTGGTAATCGGTGT+CGG | 0.558677 | 3:-100674119 | None:intergenic |
| CGTGCAACACGTTGTTGTTG+TGG | 0.561323 | 3:-100674377 | None:intergenic |
| AAGACATTGGTTGTAGACAA+TGG | 0.574027 | 3:-100674065 | None:intergenic |
| CGATCGCGTAGAGTCTTGGT+TGG | 0.577565 | 3:+100674237 | MsG0380017789.01.T01:CDS |
| ATAGAAAGAGTCGCTGAAGA+GGG | 0.577864 | 3:-100673972 | None:intergenic |
| GGAGGAACCTGAACCTGTCA+TGG | 0.581065 | 3:+100674327 | MsG0380017789.01.T01:CDS |
| ATTATCTCCCGTAGTTGTCA+CGG | 0.585430 | 3:-100674401 | None:intergenic |
| CGAATAACCCGAAAGAGTTG+AGG | 0.594150 | 3:+100674488 | MsG0380017789.01.T01:CDS |
| AGTAACTAACTCCGATGAAG+AGG | 0.607096 | 3:-100674095 | None:intergenic |
| ATATGGAGAGAATGATAACA+TGG | 0.610639 | 3:-100674009 | None:intergenic |
| AATTTCCTAATATGGAGTCA+GGG | 0.612963 | 3:-100674677 | None:intergenic |
| CAACCAAGACTCTACGCGAT+CGG | 0.620858 | 3:-100674236 | None:intergenic |
| GGGAGATAATAAGGTTTCGG+AGG | 0.625066 | 3:+100674414 | MsG0380017789.01.T01:CDS |
| TAAAGAGGAGTTCAAGCGCA+CGG | 0.627740 | 3:+100674576 | MsG0380017789.01.T01:CDS |
| CATGGCAGTTACTGAGACCG+CGG | 0.630430 | 3:+100674345 | MsG0380017789.01.T01:CDS |
| TGAAAAGGATTTGAGAGCGA+TGG | 0.632108 | 3:-100673927 | None:intergenic |
| TTCAATCGGAGTGTTATGAA+CGG | 0.647297 | 3:+100674455 | MsG0380017789.01.T01:CDS |
| GTGTTATGAACGGAGTCTGG+TGG | 0.647712 | 3:+100674465 | MsG0380017789.01.T01:CDS |
| TGAAGAAGAAGGAACCGCAG+AGG | 0.656653 | 3:+100674527 | MsG0380017789.01.T01:CDS |
| TAAGAAGATGATGCAATGGG+AGG | 0.667634 | 3:+100674621 | MsG0380017789.01.T01:CDS |
| TGCAATGGGAGGAACAAGCA+CGG | 0.671220 | 3:+100674632 | MsG0380017789.01.T01:CDS |
| AAATGTATTCAAGGTTACGA+CGG | 0.671817 | 3:-100673847 | None:intergenic |
| TGTTGTGGTCTCAGTAACCG+CGG | 0.693857 | 3:-100674362 | None:intergenic |
| ACATTGGTTGTAGACAATGG+TGG | 0.711206 | 3:-100674062 | None:intergenic |
| ACATAACACAAACTCCTCTG+CGG | 0.740468 | 3:-100674541 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTAAAACATTGGTAAT+CGG | - | Chr3:100674128-100674147 | None:intergenic | 15.0% |
| !!! | ACAACAAAATTTTTGCAATT+CGG | + | Chr3:100674264-100674283 | MsG0380017789.01.T01:CDS | 20.0% |
| ! | TCTTAAAATAACCTCTTCAT+CGG | + | Chr3:100674084-100674103 | MsG0380017789.01.T01:CDS | 25.0% |
| !!! | AAGAGGTTATTTTAAGACAT+TGG | - | Chr3:100674081-100674100 | None:intergenic | 25.0% |
| !!! | ATGTTTTAAAATAACCGCAA+AGG | + | Chr3:100674135-100674154 | MsG0380017789.01.T01:CDS | 25.0% |
| !!! | TTGCGGTTATTTTAAAACAT+TGG | - | Chr3:100674135-100674154 | None:intergenic | 25.0% |
| AAATGTATTCAAGGTTACGA+CGG | - | Chr3:100673850-100673869 | None:intergenic | 30.0% | |
| AACATAAGAAGATGATGCAA+TGG | + | Chr3:100674617-100674636 | MsG0380017789.01.T01:CDS | 30.0% | |
| ACAAGTGGAAAATGTATTCA+AGG | - | Chr3:100673859-100673878 | None:intergenic | 30.0% | |
| ACATAAGAAGATGATGCAAT+GGG | + | Chr3:100674618-100674637 | MsG0380017789.01.T01:CDS | 30.0% | |
| ATATGGAGAGAATGATAACA+TGG | - | Chr3:100674012-100674031 | None:intergenic | 30.0% | |
| TGTTGAAGAATTGAGTAAAG+AGG | + | Chr3:100674561-100674580 | MsG0380017789.01.T01:CDS | 30.0% | |
| TTGGTTGGAGAGTTTATATA+TGG | - | Chr3:100674029-100674048 | None:intergenic | 30.0% | |
| ! | AATTTTTGCAATTCGGAAGT+TGG | + | Chr3:100674271-100674290 | MsG0380017789.01.T01:CDS | 30.0% |
| CAACACATGTTTGAAGAAGA+AGG | + | Chr3:100674516-100674535 | MsG0380017789.01.T01:CDS | 35.0% | |
| TAAAATAACCGCAAAGGAAC+CGG | + | Chr3:100674141-100674160 | MsG0380017789.01.T01:CDS | 35.0% | |
| TACGGGAGATAATAAGGTTT+CGG | + | Chr3:100674411-100674430 | MsG0380017789.01.T01:CDS | 35.0% | |
| TTGAATCTCCTCAACTCTTT+CGG | - | Chr3:100674499-100674518 | None:intergenic | 35.0% | |
| ! | AAAACATTGGTAATCGGTGT+CGG | - | Chr3:100674122-100674141 | None:intergenic | 35.0% |
| ! | AAGACATTGGTTGTAGACAA+TGG | - | Chr3:100674068-100674087 | None:intergenic | 35.0% |
| ! | AGATTCGTCTCTGATTTTTG+AGG | + | Chr3:100674294-100674313 | MsG0380017789.01.T01:CDS | 35.0% |
| ! | TTCAATCGGAGTGTTATGAA+CGG | + | Chr3:100674455-100674474 | MsG0380017789.01.T01:CDS | 35.0% |
| AAAGCAAGAGAGTGGCAAAA+AGG | - | Chr3:100673886-100673905 | None:intergenic | 40.0% | |
| ACATAACACAAACTCCTCTG+CGG | - | Chr3:100674544-100674563 | None:intergenic | 40.0% | |
| AGTAACTAACTCCGATGAAG+AGG | - | Chr3:100674098-100674117 | None:intergenic | 40.0% | |
| ATAGAAAGAGTCGCTGAAGA+GGG | - | Chr3:100673975-100673994 | None:intergenic | 40.0% | |
| ATTATCTCCCGTAGTTGTCA+CGG | - | Chr3:100674404-100674423 | None:intergenic | 40.0% | |
| GACAACTACGGGAGATAATA+AGG | + | Chr3:100674405-100674424 | MsG0380017789.01.T01:CDS | 40.0% | |
| GTGTTACAGGAGAGTTCAAT+CGG | + | Chr3:100674441-100674460 | MsG0380017789.01.T01:CDS | 40.0% | |
| TAAGAAGATGATGCAATGGG+AGG | + | Chr3:100674621-100674640 | MsG0380017789.01.T01:CDS | 40.0% | |
| TGAAAAGGATTTGAGAGCGA+TGG | - | Chr3:100673930-100673949 | None:intergenic | 40.0% | |
| TGAATCTCCTCAACTCTTTC+GGG | - | Chr3:100674498-100674517 | None:intergenic | 40.0% | |
| TGGCAAAAAGGTTGAACAAG+TGG | - | Chr3:100673874-100673893 | None:intergenic | 40.0% | |
| ! | AAGAGCGTAGAGATGTGAAA+AGG | - | Chr3:100673945-100673964 | None:intergenic | 40.0% |
| ! | ACATTGGTTGTAGACAATGG+TGG | - | Chr3:100674065-100674084 | None:intergenic | 40.0% |
| ! | GTCTCAGAAAACAGAGTACT+TGG | + | Chr3:100674654-100674673 | MsG0380017789.01.T01:CDS | 40.0% |
| !! | GATTTTTGAGGAAACTGTCG+CGG | + | Chr3:100674306-100674325 | MsG0380017789.01.T01:CDS | 40.0% |
| !! | GGATCTTGTTGTTGTTTCTC+AGG | - | Chr3:100674178-100674197 | None:intergenic | 40.0% |
| !! | TTTCGGAGGAAAAGTGTTAC+AGG | + | Chr3:100674428-100674447 | MsG0380017789.01.T01:CDS | 40.0% |
| !!! | TTGCCACTCTCTTGCTTTTT+TGG | + | Chr3:100673888-100673907 | MsG0380017789.01.T01:CDS | 40.0% |
| AGATCCTGTAGTGTCTTCGT+TGG | + | Chr3:100674192-100674211 | MsG0380017789.01.T01:CDS | 45.0% | |
| CATAGAAAGAGTCGCTGAAG+AGG | - | Chr3:100673976-100673995 | None:intergenic | 45.0% | |
| CGAATAACCCGAAAGAGTTG+AGG | + | Chr3:100674488-100674507 | MsG0380017789.01.T01:CDS | 45.0% | |
| GGGAGATAATAAGGTTTCGG+AGG | + | Chr3:100674414-100674433 | MsG0380017789.01.T01:CDS | 45.0% | |
| GTGCCAAAAAAGCAAGAGAG+TGG | - | Chr3:100673894-100673913 | None:intergenic | 45.0% | |
| TAAAGAGGAGTTCAAGCGCA+CGG | + | Chr3:100674576-100674595 | MsG0380017789.01.T01:CDS | 45.0% | |
| TTCTCAGGCAAAGACTCTAC+CGG | - | Chr3:100674163-100674182 | None:intergenic | 45.0% | |
| TTCTCCAACGAAGACACTAC+AGG | - | Chr3:100674199-100674218 | None:intergenic | 45.0% | |
| AGAGTCGCTGAAGAGGGATT+TGG | - | Chr3:100673969-100673988 | None:intergenic | 50.0% | |
| CAACCAAGACTCTACGCGAT+CGG | - | Chr3:100674239-100674258 | None:intergenic | 50.0% | |
| GTAACTGCCATGACAGGTTC+AGG | - | Chr3:100674337-100674356 | None:intergenic | 50.0% | |
| GTCTCAGTAACTGCCATGAC+AGG | - | Chr3:100674343-100674362 | None:intergenic | 50.0% | |
| GTGTTATGAACGGAGTCTGG+TGG | + | Chr3:100674465-100674484 | MsG0380017789.01.T01:CDS | 50.0% | |
| TGAAGAAGAAGGAACCGCAG+AGG | + | Chr3:100674527-100674546 | MsG0380017789.01.T01:CDS | 50.0% | |
| TGCAATGGGAGGAACAAGCA+CGG | + | Chr3:100674632-100674651 | MsG0380017789.01.T01:CDS | 50.0% | |
| TGTTGTGGTCTCAGTAACCG+CGG | - | Chr3:100674365-100674384 | None:intergenic | 50.0% | |
| TTACCGATCGCGTAGAGTCT+TGG | + | Chr3:100674233-100674252 | MsG0380017789.01.T01:CDS | 50.0% | |
| TTGCACGACCGTGACAACTA+CGG | + | Chr3:100674393-100674412 | MsG0380017789.01.T01:CDS | 50.0% | |
| ! | CGTGCAACACGTTGTTGTTG+TGG | - | Chr3:100674380-100674399 | None:intergenic | 50.0% |
| ! | GGAGTGTTATGAACGGAGTC+TGG | + | Chr3:100674462-100674481 | MsG0380017789.01.T01:CDS | 50.0% |
| ! | TGGTGGAAACGTCGTCGTTT+TGG | - | Chr3:100674048-100674067 | None:intergenic | 50.0% |
| !! | AGACTCTACCGGTTCCTTTG+CGG | - | Chr3:100674152-100674171 | None:intergenic | 50.0% |
| !! | GGAAACGTCGTCGTTTTGGT+TGG | - | Chr3:100674044-100674063 | None:intergenic | 50.0% |
| !! | TTTTGAGGAAACTGTCGCGG+AGG | + | Chr3:100674309-100674328 | MsG0380017789.01.T01:CDS | 50.0% |
| CATGGCAGTTACTGAGACCG+CGG | + | Chr3:100674345-100674364 | MsG0380017789.01.T01:CDS | 55.0% | |
| CGATCGCGTAGAGTCTTGGT+TGG | + | Chr3:100674237-100674256 | MsG0380017789.01.T01:CDS | 55.0% | |
| GACTCTACGCGATCGGTAAC+AGG | - | Chr3:100674232-100674251 | None:intergenic | 55.0% | |
| GGAGGAACCTGAACCTGTCA+TGG | + | Chr3:100674327-100674346 | MsG0380017789.01.T01:CDS | 55.0% | |
| TCGCGTAGAGTCTTGGTTGG+AGG | + | Chr3:100674240-100674259 | MsG0380017789.01.T01:CDS | 55.0% | |
| TGCACGACCGTGACAACTAC+GGG | + | Chr3:100674394-100674413 | MsG0380017789.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 100673827 | 100674693 | 100673827 | ID=MsG0380017789.01;Name=MsG0380017789.01 |
| Chr3 | mRNA | 100673827 | 100674693 | 100673827 | ID=MsG0380017789.01.T01;Parent=MsG0380017789.01;Name=MsG0380017789.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|288 |
| Chr3 | exon | 100673827 | 100674693 | 100673827 | ID=MsG0380017789.01.T01:exon:35823;Parent=MsG0380017789.01.T01 |
| Chr3 | CDS | 100673827 | 100674693 | 100673827 | ID=MsG0380017789.01.T01:cds;Parent=MsG0380017789.01.T01 |
| Gene Sequence |
| Protein sequence |
