AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018093.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018093.01.T01 MTR_4g005190 100.000 206 0 0 10 215 545 750 3.60e-147 432
MsG0480018093.01.T01 MTR_4g005270 88.350 206 24 0 10 215 545 750 1.65e-131 391
MsG0480018093.01.T01 MTR_0013s0230 84.058 207 32 1 10 215 545 751 8.75e-126 377
MsG0480018093.01.T01 MTR_8g016975 81.553 206 38 0 10 215 545 750 3.98e-122 367
MsG0480018093.01.T01 MTR_8g016960 81.068 206 39 0 10 215 540 745 6.62e-122 367
MsG0480018093.01.T01 MTR_8g016940 77.184 206 47 0 10 215 54 259 1.99e-118 341
MsG0480018093.01.T01 MTR_8g017020 78.641 206 44 0 10 215 511 716 4.25e-118 356
MsG0480018093.01.T01 MTR_3g436160 83.981 206 33 0 10 215 484 689 5.37e-117 352
MsG0480018093.01.T01 MTR_7g034535 80.193 207 40 1 10 215 480 686 7.41e-117 352
MsG0480018093.01.T01 MTR_6g093180 61.429 210 81 0 11 220 543 752 1.82e-92 290
MsG0480018093.01.T01 MTR_5g008810 62.621 206 77 0 10 215 543 748 1.31e-90 285
MsG0480018093.01.T01 MTR_6g035010 60.976 205 80 0 11 215 543 747 1.37e-88 280
MsG0480018093.01.T01 MTR_6g034985 59.512 205 83 0 11 215 543 747 4.26e-85 271
MsG0480018093.01.T01 MTR_7g062020 57.767 206 86 1 11 215 555 760 4.30e-84 268
MsG0480018093.01.T01 MTR_6g088140 58.738 206 84 1 11 215 528 733 1.01e-83 267
MsG0480018093.01.T01 MTR_6g093100 57.009 214 83 2 11 215 554 767 2.33e-81 261
MsG0480018093.01.T01 MTR_4g005190 98.400 125 2 0 10 134 545 669 4.55e-81 258
MsG0480018093.01.T01 MTR_6g088140 55.556 126 55 1 11 135 528 653 1.11e-42 155
MsG0480018093.01.T01 MTR_8g016570 73.214 56 15 0 160 215 28 83 4.02e-24 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018093.01.T01 AT1G78955 75.728 206 50 0 10 215 371 576 6.10e-114 341
MsG0480018093.01.T01 AT1G78955 75.728 206 50 0 10 215 481 686 1.02e-112 341
MsG0480018093.01.T01 AT1G78955 75.728 206 50 0 10 215 546 751 5.31e-112 341
MsG0480018093.01.T01 AT1G78960 71.359 206 59 0 10 215 395 600 6.89e-106 321
MsG0480018093.01.T01 AT1G78960 71.359 206 59 0 10 215 546 751 2.43e-104 321
MsG0480018093.01.T01 AT1G78960 71.359 206 59 0 10 215 546 751 2.43e-104 321
MsG0480018093.01.T01 AT1G78960 71.359 206 59 0 10 215 546 751 2.43e-104 321
MsG0480018093.01.T01 AT1G78970 69.668 211 64 0 10 220 543 753 1.32e-103 319
MsG0480018093.01.T01 AT1G78970 69.668 211 64 0 10 220 543 753 1.32e-103 319
MsG0480018093.01.T01 AT1G78970 69.668 211 64 0 10 220 543 753 1.32e-103 319
MsG0480018093.01.T01 AT1G78950 72.330 206 57 0 10 215 555 760 1.16e-99 309
MsG0480018093.01.T01 AT1G78950 72.330 206 57 0 10 215 546 751 1.37e-99 309
MsG0480018093.01.T01 AT1G78955 75.843 178 43 0 10 187 481 658 1.52e-94 293
MsG0480018093.01.T01 AT1G66960 64.563 206 73 0 10 215 546 751 1.65e-94 296
MsG0480018093.01.T01 AT1G66960 64.563 206 73 0 10 215 546 751 1.65e-94 296
MsG0480018093.01.T01 AT1G66960 64.563 206 73 0 10 215 546 751 1.65e-94 296
MsG0480018093.01.T01 AT1G66960 64.563 206 73 0 10 215 555 760 1.81e-94 296
MsG0480018093.01.T01 AT5G48010 60.748 214 84 0 11 224 552 765 1.23e-90 285
MsG0480018093.01.T01 AT2G07050 60.369 217 84 1 10 226 452 666 4.14e-90 282
MsG0480018093.01.T01 AT4G15370 60.952 210 82 0 14 223 547 756 4.16e-90 284
MsG0480018093.01.T01 AT4G15370 60.952 210 82 0 14 223 550 759 4.24e-90 284
MsG0480018093.01.T01 AT2G07050 60.369 217 84 1 10 226 543 757 1.96e-89 282
MsG0480018093.01.T01 AT1G78500 64.706 204 72 0 11 214 553 756 2.25e-89 282
MsG0480018093.01.T01 AT1G78500 64.706 204 72 0 11 214 553 756 2.25e-89 282
MsG0480018093.01.T01 AT4G15340 62.255 204 77 0 11 214 552 755 1.86e-87 277
MsG0480018093.01.T01 AT3G29255 60.784 204 80 0 11 214 493 696 8.07e-87 274
MsG0480018093.01.T01 AT5G36150 59.314 204 83 0 11 214 547 750 9.57e-87 275
MsG0480018093.01.T01 AT5G36150 59.314 204 83 0 11 214 547 750 9.57e-87 275
MsG0480018093.01.T01 AT1G78960 70.787 178 52 0 10 187 546 723 1.26e-86 274
MsG0480018093.01.T01 AT5G48010 61.307 199 77 0 26 224 559 757 7.37e-84 268
MsG0480018093.01.T01 AT3G45130 58.252 206 86 0 10 215 543 748 3.10e-83 266
MsG0480018093.01.T01 AT3G45130 58.252 206 86 0 10 215 543 748 3.10e-83 266
MsG0480018093.01.T01 AT3G45130 58.252 206 86 0 10 215 543 748 3.10e-83 266
MsG0480018093.01.T01 AT3G45130 58.252 206 86 0 10 215 543 748 3.67e-83 266
MsG0480018093.01.T01 AT3G45130 58.252 206 86 0 10 215 543 748 3.67e-83 266
MsG0480018093.01.T01 AT5G42600 60.784 204 79 1 11 214 548 750 4.76e-83 265
MsG0480018093.01.T01 AT1G78955 73.016 126 34 0 10 135 481 606 2.50e-60 202

Find 94 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAAATACGAACCTTCTT+TGG 0.178051 4:-151519 None:intergenic
TTTGTTTCACTTATGGTTCT+TGG 0.208708 4:+151367 MsG0480018093.01.T01:CDS
ACATACTGCATGGGCTCTTA+TGG 0.219444 4:+151654 MsG0480018093.01.T01:CDS
TTAGAAACTAAGCAACTAAA+AGG 0.250761 4:-154001 None:intergenic
TGGGGAGTTTGTTTCACTTA+TGG 0.288057 4:+151360 MsG0480018093.01.T01:CDS
AAGTAAAAGATGAATGTTTA+TGG 0.290115 4:-154059 None:intergenic
ATGGTTCATGGTATGGAAAC+TGG 0.321747 4:+151340 MsG0480018093.01.T01:CDS
AATAAATACGAACCTTCTTT+GGG 0.337567 4:-151518 None:intergenic
GCCAATCGCCTTCTTCCAAC+TGG 0.338128 4:-151750 None:intergenic
CATACTGCATGGGCTCTTAT+GGG 0.340268 4:+151655 MsG0480018093.01.T01:CDS
AAAAGCAGCAAAAGAGGCTC+TGG 0.371603 4:-153936 None:intergenic
ATCTCTTTCTTCCTATGCCC+TGG 0.383363 4:-151264 None:intergenic
CAGAGAGAGGATGGTGGGTG+GGG 0.396519 4:+151477 MsG0480018093.01.T01:CDS
AGAGAGAGGATGGTGGGTGG+GGG 0.400405 4:+151478 MsG0480018093.01.T01:CDS
TATGGTTCTTGGTTTGCTCT+TGG 0.409666 4:+151378 MsG0480018093.01.T01:CDS
TACAAACAGCCGATGGTTCA+TGG 0.415211 4:+151328 MsG0480018093.01.T01:CDS
TTTGCGAATAGCAGCGCAAT+TGG 0.416657 4:-151430 None:intergenic
TAAAACGGTAAAGCTGCAAC+AGG 0.419021 4:-153977 None:intergenic
GAGAGAGGATGGTGGGTGGG+GGG 0.421109 4:+151479 MsG0480018093.01.T01:CDS
AGTGCGAAAATACGAAGATA+TGG 0.423359 4:+153839 MsG0480018093.01.T01:CDS
AAAGCTGCAACAGGGTAACT+TGG 0.429274 4:-153968 None:intergenic
CTTGGTGGTTTAGCAGCTGC+TGG 0.433156 4:+151396 MsG0480018093.01.T01:CDS
GGTAAATATCTCTATACATT+GGG 0.445154 4:-152265 None:intergenic
GGTTCTTGGTTTGCTCTTGG+TGG 0.447161 4:+151381 MsG0480018093.01.T01:CDS
AGGATTGAGATGAACCTGTT+GGG 0.456514 4:-151773 None:intergenic
GTGCCAAGCTTCTGACTTGG+TGG 0.463887 4:-153877 None:intergenic
TTGTTCAAGAAGCTATATCC+AGG 0.467138 4:+151246 MsG0480018093.01.T01:CDS
GCAAAAGAGGCTCTGGGAGG+TGG 0.467341 4:-153929 None:intergenic
AGAAGGCGATTGGCCCCAAC+AGG 0.467418 4:+151759 MsG0480018093.01.T01:CDS
GGGTTTAATTCATGCCGGCC+AGG 0.479935 4:+151675 MsG0480018093.01.T01:CDS
TAGAGATATTTACCCCTTGT+GGG 0.480496 4:+152274 MsG0480018093.01.T01:CDS
CTATCTTTCAAGCCCAAAGA+AGG 0.480841 4:+151506 MsG0480018093.01.T01:CDS
GCAGCACGATGGAGAGGAGT+AGG 0.495145 4:-151709 None:intergenic
ACAGAGAGAGGATGGTGGGT+GGG 0.500291 4:+151476 MsG0480018093.01.T01:CDS
GAGTAGGGTCTCTCTCTGCC+TGG 0.514588 4:-151693 None:intergenic
ATCAATTCCCAGTTGGAAGA+AGG 0.521619 4:+151742 MsG0480018093.01.T01:CDS
TTGGCAGCATGGGAGCCCGC+AGG 0.523182 4:+151031 None:intergenic
CCCAGTTGGAAGAAGGCGAT+TGG 0.523228 4:+151749 MsG0480018093.01.T01:CDS
GGGTAAATATCTCTATACAT+TGG 0.526077 4:-152266 None:intergenic
ACAGCCGATGGTTCATGGTA+TGG 0.527570 4:+151333 MsG0480018093.01.T01:CDS
AAGCAACTAAAAGGGTAAAA+CGG 0.532389 4:-153992 None:intergenic
AACCACTCTTGAGCTCCTGC+GGG 0.532584 4:-151046 None:intergenic
AGGGTCTCTCTCTGCCTGGC+CGG 0.534032 4:-151689 None:intergenic
TAACCACTCTTGAGCTCCTG+CGG 0.534900 4:-151047 None:intergenic
CATGAATATGTTGAGTGCAC+TGG 0.536807 4:+151201 MsG0480018093.01.T01:CDS
TAGAAACTAAGCAACTAAAA+GGG 0.539326 4:-154000 None:intergenic
CTTATGGGTTTAATTCATGC+CGG 0.540134 4:+151670 MsG0480018093.01.T01:CDS
GCCGTGCCACATGTTGGCAC+TGG 0.540574 4:-153902 None:intergenic
AGAGGCTCTGGGAGGTGGCA+CGG 0.544673 4:-153924 None:intergenic
TATTTATGGCAGGAAATCAC+AGG 0.544762 4:+152212 MsG0480018093.01.T01:intron
ATAGAGATATTTACCCCTTG+TGG 0.545304 4:+152273 MsG0480018093.01.T01:CDS
CCTCTCTCTGTGTTGTGAGA+AGG 0.545702 4:-151464 None:intergenic
TGGTTCATGGTATGGAAACT+GGG 0.547996 4:+151341 MsG0480018093.01.T01:CDS
TTCAATGAATCGAACTGCCT+CGG 0.553806 4:-151301 None:intergenic
TTCCCACCAAGTCAGAAGCT+TGG 0.567540 4:+153874 MsG0480018093.01.T01:CDS
CCAATGTTGTACATACTGCA+TGG 0.568294 4:+151644 MsG0480018093.01.T01:CDS
ATTGCTCATCAATTCCCAGT+TGG 0.571873 4:+151735 MsG0480018093.01.T01:CDS
TAAAAGATGAATGTTTATGG+TGG 0.573498 4:-154056 None:intergenic
AGAAGCTATATCCAGGGCAT+AGG 0.579101 4:+151253 MsG0480018093.01.T01:CDS
TGAAGCAAAAGAAAGAGGAC+TGG 0.579472 4:+153807 MsG0480018093.01.T01:CDS
CTCACAACACAGAGAGAGGA+TGG 0.580995 4:+151468 MsG0480018093.01.T01:CDS
CAATGTTGTACATACTGCAT+GGG 0.582226 4:+151645 MsG0480018093.01.T01:CDS
GCCGTGCCAAGCTTCTGACT+TGG 0.582353 4:-153880 None:intergenic
GGCACGGCCGTGCCACATGT+TGG 0.584181 4:-153908 None:intergenic
CACAGAGAGAGGATGGTGGG+TGG 0.585302 4:+151475 MsG0480018093.01.T01:CDS
AAGCTGCAACAGGGTAACTT+GGG 0.587165 4:-153967 None:intergenic
CCAATCGCCTTCTTCCAACT+GGG 0.588505 4:-151749 None:intergenic
TGTCTTGAAGCAAAAGAAAG+AGG 0.588846 4:+153802 MsG0480018093.01.T01:CDS
AGCCCGCAGGAGCTCAAGAG+TGG 0.590345 4:+151044 MsG0480018093.01.T01:CDS
TGCCAAGCTTCTGACTTGGT+GGG 0.590863 4:-153876 None:intergenic
CCTTCTCACAACACAGAGAG+AGG 0.594275 4:+151464 MsG0480018093.01.T01:CDS
AGGAACGAATATATGGTGGA+AGG 0.599653 4:+153779 MsG0480018093.01.T01:intron
GGATTGAGATGAACCTGTTG+GGG 0.601251 4:-151772 None:intergenic
CAACACAGAGAGAGGATGGT+GGG 0.605076 4:+151472 MsG0480018093.01.T01:CDS
CAGCACGATGGAGAGGAGTA+GGG 0.609931 4:-151708 None:intergenic
TGTTCAAGAAGCTATATCCA+GGG 0.610050 4:+151247 MsG0480018093.01.T01:CDS
GCCAGTGCCAACATGTGGCA+CGG 0.611039 4:+153901 MsG0480018093.01.T01:CDS
CCATGCAGTATGTACAACAT+TGG 0.611660 4:-151644 None:intergenic
AAAGCAGCAAAAGAGGCTCT+GGG 0.615426 4:-153935 None:intergenic
CAGATATATGTACCTCTCGA+AGG 0.616858 4:+151613 MsG0480018093.01.T01:intron
ACCAAGTCAGAAGCTTGGCA+CGG 0.618933 4:+153879 MsG0480018093.01.T01:CDS
GCACGGCCAGTGCCAACATG+TGG 0.630678 4:+153896 MsG0480018093.01.T01:CDS
TTGGATCGGCTTCCTTCGAG+AGG 0.631858 4:-151625 None:intergenic
CAGTATGTACAACATTGGAT+CGG 0.635228 4:-151639 None:intergenic
GTTTCCATACCATGAACCAT+CGG 0.636194 4:-151337 None:intergenic
ATACTCGGCTAGAGCCCACA+AGG 0.640192 4:-152288 None:intergenic
GATAGAGAATTTCATCTCCG+AGG 0.658691 4:+151284 MsG0480018093.01.T01:CDS
GAAGATATACAAACAGCCGA+TGG 0.667706 4:+151321 MsG0480018093.01.T01:CDS
ACAACACAGAGAGAGGATGG+TGG 0.668505 4:+151471 MsG0480018093.01.T01:CDS
GGTTCATGGTATGGAAACTG+GGG 0.686882 4:+151342 MsG0480018093.01.T01:CDS
AAAACGGTAAAGCTGCAACA+GGG 0.707365 4:-153976 None:intergenic
TACTCGGCTAGAGCCCACAA+GGG 0.715735 4:-152287 None:intergenic
ACTCGGCTAGAGCCCACAAG+GGG 0.728980 4:-152286 None:intergenic
GCAGCAAAAGAGGCTCTGGG+AGG 0.749841 4:-153932 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAACTAAACTAAATA+AGG - Chr4:153684-153703 None:intergenic 10.0%
!! ACAAAACAAAAATTAATAAT+AGG - Chr4:152190-152209 None:intergenic 10.0%
!!! AATTTGATTTTTTTTTTTCT+TGG - Chr4:151952-151971 None:intergenic 10.0%
!!! ATTGTCTTTTATTTTATTTT+AGG + Chr4:153759-153778 MsG0480018093.01.T01:intron 10.0%
!!! CATTCTTTTTTATTTTAAAA+TGG - Chr4:152855-152874 None:intergenic 10.0%
!! AGTAAATTAATCAAAAAGTT+TGG + Chr4:153385-153404 MsG0480018093.01.T01:intron 15.0%
!! GTATTTATTAATTGTTTAGA+TGG - Chr4:152168-152187 None:intergenic 15.0%
!! TAGCTGAAAATAAATAATAA+AGG - Chr4:151142-151161 None:intergenic 15.0%
!! TATTAATATATACTCATTCA+TGG + Chr4:151083-151102 MsG0480018093.01.T01:intron 15.0%
!! TATTCTTAATGAAATGATAT+TGG - Chr4:153267-153286 None:intergenic 15.0%
!! TTTCTAAATAAGTATATCAA+AGG - Chr4:151796-151815 None:intergenic 15.0%
!!! CATATTTTTAATGACATTTT+AGG + Chr4:152704-152723 MsG0480018093.01.T01:intron 15.0%
!!! TTTTTGATTAATTTACTATG+AGG - Chr4:153382-153401 None:intergenic 15.0%
!!! TTTTTGTTTTGTCTTATTTA+TGG + Chr4:152198-152217 MsG0480018093.01.T01:intron 15.0%
!!! TTTTTTATTCAAGAAAGTTT+AGG - Chr4:153077-153096 None:intergenic 15.0%
!! AAGTAAAAGATGAATGTTTA+TGG - Chr4:154062-154081 None:intergenic 20.0%
!! ACAAAAATGCCAAAATTATT+AGG + Chr4:153132-153151 MsG0480018093.01.T01:intron 20.0%
!! ATATGAAAACAGGAATTTAT+AGG - Chr4:152689-152708 None:intergenic 20.0%
!! CAAATAAAACCAAATAAGTT+GGG + Chr4:152414-152433 MsG0480018093.01.T01:intron 20.0%
!! GAAACAAATAAAATGTTTCA+AGG - Chr4:152124-152143 None:intergenic 20.0%
!! GATTATTTGATTCTTATACA+AGG + Chr4:152912-152931 MsG0480018093.01.T01:intron 20.0%
!! GTCATTAAAAATATGAAAAC+AGG - Chr4:152699-152718 None:intergenic 20.0%
!! TACTTATTTAGAAATGAATG+TGG + Chr4:151802-151821 MsG0480018093.01.T01:intron 20.0%
!! TCAAATAAAACCAAATAAGT+TGG + Chr4:152413-152432 MsG0480018093.01.T01:intron 20.0%
!! TGATTCTTTCTTTATTGATA+TGG - Chr4:152392-152411 None:intergenic 20.0%
!! TGTTTAGTTATTATCCTAAA+AGG + Chr4:152783-152802 MsG0480018093.01.T01:intron 20.0%
!! TTAGTTTATCAACTAATGAA+TGG - Chr4:151116-151135 None:intergenic 20.0%
!!! AAAAAAAAAAGAGTTTTCAG+CGG - Chr4:152821-152840 None:intergenic 20.0%
!!! AACGAAGATTGATTTTTTTA+AGG + Chr4:153414-153433 MsG0480018093.01.T01:intron 20.0%
!!! TTATTTTAGGAACGAATATA+TGG + Chr4:153772-153791 MsG0480018093.01.T01:intron 20.0%
! AATAAATACGAACCTTCTTT+GGG - Chr4:151521-151540 None:intergenic 25.0%
! AATCAACACATGTAGAAAAT+AGG - Chr4:152448-152467 None:intergenic 25.0%
! AATTTGACATCTTAACTATG+AGG + Chr4:151911-151930 MsG0480018093.01.T01:intron 25.0%
! AGCATTATATAATCAAGTAC+AGG + Chr4:151851-151870 MsG0480018093.01.T01:intron 25.0%
! ATAAACTAACACACATAAAG+TGG - Chr4:153194-153213 None:intergenic 25.0%
! ATTCAAGAAAGTTTAGGAAT+TGG - Chr4:153071-153090 None:intergenic 25.0%
! ATTTGATTCTTATACAAGGA+CGG + Chr4:152916-152935 MsG0480018093.01.T01:intron 25.0%
! GGTAAATATCTCTATACATT+GGG - Chr4:152268-152287 None:intergenic 25.0%
! TAAAAGATGAATGTTTATGG+TGG - Chr4:154059-154078 None:intergenic 25.0%
! TAATAAATACGAACCTTCTT+TGG - Chr4:151522-151541 None:intergenic 25.0%
! TAGAAACTAAGCAACTAAAA+GGG - Chr4:154003-154022 None:intergenic 25.0%
! TTAGAAACTAAGCAACTAAA+AGG - Chr4:154004-154023 None:intergenic 25.0%
! TTATTGTCAATTCATGAAGT+CGG - Chr4:151567-151586 None:intergenic 25.0%
! TTCAAGAAAGTTTAGGAATT+GGG - Chr4:153070-153089 None:intergenic 25.0%
! TTTCAGTTTGATGTTGAAAT+CGG + Chr4:152038-152057 MsG0480018093.01.T01:intron 25.0%
!! AAAAAAAAAGAGTTTTCAGC+GGG - Chr4:152820-152839 None:intergenic 25.0%
!! GAATTGACTCATCAATAAAT+TGG + Chr4:152969-152988 MsG0480018093.01.T01:intron 25.0%
!!! AAAATGTTTCAAGGTTTTGA+TGG - Chr4:152115-152134 None:intergenic 25.0%
!!! AATGTTTGAGTTTTGAAATG+AGG - Chr4:152066-152085 None:intergenic 25.0%
AAATGCCAAAATTATTAGGC+CGG + Chr4:153136-153155 MsG0480018093.01.T01:intron 30.0%
AAGCAACTAAAAGGGTAAAA+CGG - Chr4:153995-154014 None:intergenic 30.0%
AGAATATTAGCTGAAATACG+AGG + Chr4:153281-153300 MsG0480018093.01.T01:intron 30.0%
CCAATTTAGAAATGTAAACG+TGG - Chr4:152524-152543 None:intergenic 30.0%
CCACGTTTACATTTCTAAAT+TGG + Chr4:152521-152540 MsG0480018093.01.T01:intron 30.0%
GGGTAAATATCTCTATACAT+TGG - Chr4:152269-152288 None:intergenic 30.0%
TCACTATGCAAGTATCTATA+AGG - Chr4:151881-151900 None:intergenic 30.0%
TGAAAACAGGAATTTATAGG+AGG - Chr4:152686-152705 None:intergenic 30.0%
TTGTTTGAACTCATTCTTTC+TGG - Chr4:152557-152576 None:intergenic 30.0%
TTTGTTTCACTTATGGTTCT+TGG + Chr4:151367-151386 MsG0480018093.01.T01:CDS 30.0%
! ATTTTCCAGTTAAATCACAG+TGG - Chr4:152336-152355 None:intergenic 30.0%
! GAGGTAGAAAGAGAAAATTT+TGG + Chr4:153300-153319 MsG0480018093.01.T01:intron 30.0%
!! TTTTAGGAACGAATATATGG+TGG + Chr4:153775-153794 MsG0480018093.01.T01:intron 30.0%
!!! CATGGACAACTTTTTTCTTT+TGG - Chr4:152580-152599 None:intergenic 30.0%
!!! TGTTTTGTCTTATTTATGGC+AGG + Chr4:152202-152221 MsG0480018093.01.T01:intron 30.0%
AACTAACACACATAAAGTGG+AGG - Chr4:153191-153210 None:intergenic 35.0%
AATGTTGCTATATGTCCCTA+CGG - Chr4:152607-152626 None:intergenic 35.0%
AGTGCGAAAATACGAAGATA+TGG + Chr4:153839-153858 MsG0480018093.01.T01:CDS 35.0%
ATAGAGATATTTACCCCTTG+TGG + Chr4:152273-152292 MsG0480018093.01.T01:CDS 35.0%
CAATGTTGTACATACTGCAT+GGG + Chr4:151645-151664 MsG0480018093.01.T01:CDS 35.0%
CAGTATGTACAACATTGGAT+CGG - Chr4:151642-151661 None:intergenic 35.0%
CTTATGGGTTTAATTCATGC+CGG + Chr4:151670-151689 MsG0480018093.01.T01:CDS 35.0%
GTGGTCATAAAAAACTAGAC+AGG + Chr4:151821-151840 MsG0480018093.01.T01:intron 35.0%
GTTTAGCGAGCACTAATTAA+TGG - Chr4:153017-153036 None:intergenic 35.0%
TAGAGATATTTACCCCTTGT+GGG + Chr4:152274-152293 MsG0480018093.01.T01:CDS 35.0%
TATAAGCTATGAGCACAAAG+AGG + Chr4:152360-152379 MsG0480018093.01.T01:intron 35.0%
TATTTATGGCAGGAAATCAC+AGG + Chr4:152212-152231 MsG0480018093.01.T01:intron 35.0%
TCAGTTGAGTTAGGACTTAT+GGG - Chr4:153113-153132 None:intergenic 35.0%
TGTCTTGAAGCAAAAGAAAG+AGG + Chr4:153802-153821 MsG0480018093.01.T01:CDS 35.0%
TGTTCAAGAAGCTATATCCA+GGG + Chr4:151247-151266 MsG0480018093.01.T01:CDS 35.0%
TTGTTCAAGAAGCTATATCC+AGG + Chr4:151246-151265 MsG0480018093.01.T01:CDS 35.0%
! GCAACATTCAAGTACTGTAT+AGG + Chr4:152619-152638 MsG0480018093.01.T01:intron 35.0%
! GCATTTTTGTCAGTTGAGTT+AGG - Chr4:153122-153141 None:intergenic 35.0%
! GGGAAATACAGAATCCTTTT+AGG - Chr4:152800-152819 None:intergenic 35.0%
! TTGGATTTTCTTCATGCGTT+TGG - Chr4:153595-153614 None:intergenic 35.0%
!! GTTTGGTGGATTCAGAATTT+GGG - Chr4:153578-153597 None:intergenic 35.0%
!! TCAAGGTTTTGATGGAAATG+AGG - Chr4:152107-152126 None:intergenic 35.0%
!! TTTGGTGGATTCAGAATTTG+GGG - Chr4:153577-153596 None:intergenic 35.0%
!!! ACATTTTAGGATCGACTGAA+TGG + Chr4:152717-152736 MsG0480018093.01.T01:intron 35.0%
!!! CTCTTTTGCTGCTTTTTCTT+CGG + Chr4:153943-153962 MsG0480018093.01.T01:CDS 35.0%
!!! TAATCCCACTGAGTTTTTTG+CGG + Chr4:151164-151183 MsG0480018093.01.T01:CDS 35.0%
!!! TCTTTTGCTGCTTTTTCTTC+GGG + Chr4:153944-153963 MsG0480018093.01.T01:CDS 35.0%
AAAACGGTAAAGCTGCAACA+GGG - Chr4:153979-153998 None:intergenic 40.0%
AAGGATTGAGATGAACCTGT+TGG - Chr4:151777-151796 None:intergenic 40.0%
AATGTCCGCAAAAAACTCAG+TGG - Chr4:151172-151191 None:intergenic 40.0%
ACAAGGACGGAGTAAAATGA+AGG + Chr4:152929-152948 MsG0480018093.01.T01:intron 40.0%
AGGATTGAGATGAACCTGTT+GGG - Chr4:151776-151795 None:intergenic 40.0%
ATCAATTCCCAGTTGGAAGA+AGG + Chr4:151742-151761 MsG0480018093.01.T01:CDS 40.0%
ATGGTTCATGGTATGGAAAC+TGG + Chr4:151340-151359 MsG0480018093.01.T01:CDS 40.0%
ATGTCCGCAAAAAACTCAGT+GGG - Chr4:151171-151190 None:intergenic 40.0%
ATTGCTCATCAATTCCCAGT+TGG + Chr4:151735-151754 MsG0480018093.01.T01:CDS 40.0%
CAGATATATGTACCTCTCGA+AGG + Chr4:151613-151632 MsG0480018093.01.T01:intron 40.0%
CATGAATATGTTGAGTGCAC+TGG + Chr4:151201-151220 MsG0480018093.01.T01:CDS 40.0%
CCAATGTTGTACATACTGCA+TGG + Chr4:151644-151663 MsG0480018093.01.T01:CDS 40.0%
CCATGCAGTATGTACAACAT+TGG - Chr4:151647-151666 None:intergenic 40.0%
CGAAGAAAAAGCAGCAAAAG+AGG - Chr4:153945-153964 None:intergenic 40.0%
CTATCTTTCAAGCCCAAAGA+AGG + Chr4:151506-151525 MsG0480018093.01.T01:CDS 40.0%
GAAGATATACAAACAGCCGA+TGG + Chr4:151321-151340 MsG0480018093.01.T01:CDS 40.0%
GATAGAGAATTTCATCTCCG+AGG + Chr4:151284-151303 MsG0480018093.01.T01:CDS 40.0%
GGTTAGCAAAAGAGAGACTT+TGG - Chr4:153614-153633 None:intergenic 40.0%
GTCAGTTGAGTTAGGACTTA+TGG - Chr4:153114-153133 None:intergenic 40.0%
GTTTCCATACCATGAACCAT+CGG - Chr4:151340-151359 None:intergenic 40.0%
TAAAACGGTAAAGCTGCAAC+AGG - Chr4:153980-153999 None:intergenic 40.0%
TAAATCACAGTGGAAGGCAA+TGG - Chr4:152326-152345 None:intergenic 40.0%
TCAATCCACTCTGATTCTTC+TGG - Chr4:153635-153654 None:intergenic 40.0%
TCCAGTTAAATCACAGTGGA+AGG - Chr4:152332-152351 None:intergenic 40.0%
TGAAGCAAAAGAAAGAGGAC+TGG + Chr4:153807-153826 MsG0480018093.01.T01:CDS 40.0%
TGGTTCATGGTATGGAAACT+GGG + Chr4:151341-151360 MsG0480018093.01.T01:CDS 40.0%
TTCAATGAATCGAACTGCCT+CGG - Chr4:151304-151323 None:intergenic 40.0%
! AGGAACGAATATATGGTGGA+AGG + Chr4:153779-153798 MsG0480018093.01.T01:intron 40.0%
! CATGCTCTAACCTTGATGAT+TGG + Chr4:153038-153057 MsG0480018093.01.T01:intron 40.0%
! GAAGGTTGACTTAGCTGATA+AGG + Chr4:152947-152966 MsG0480018093.01.T01:intron 40.0%
! GACATCCGGCCTAATAATTT+TGG - Chr4:153144-153163 None:intergenic 40.0%
! GATTTTCTTCATGCGTTTGG+TGG - Chr4:153592-153611 None:intergenic 40.0%
! TGGGGAGTTTGTTTCACTTA+TGG + Chr4:151360-151379 MsG0480018093.01.T01:CDS 40.0%
!! CGTTTGGTGGATTCAGAATT+TGG - Chr4:153579-153598 None:intergenic 40.0%
!! TATGGTTCTTGGTTTGCTCT+TGG + Chr4:151378-151397 MsG0480018093.01.T01:CDS 40.0%
AAAAGCAGCAAAAGAGGCTC+TGG - Chr4:153939-153958 None:intergenic 45.0%
AAAGCAGCAAAAGAGGCTCT+GGG - Chr4:153938-153957 None:intergenic 45.0%
AAAGCTGCAACAGGGTAACT+TGG - Chr4:153971-153990 None:intergenic 45.0%
AAGCTGCAACAGGGTAACTT+GGG - Chr4:153970-153989 None:intergenic 45.0%
AAGTTGTCCATGTTTGCCGT+AGG + Chr4:152588-152607 MsG0480018093.01.T01:intron 45.0%
ACATACTGCATGGGCTCTTA+TGG + Chr4:151654-151673 MsG0480018093.01.T01:CDS 45.0%
AGAAGCTATATCCAGGGCAT+AGG + Chr4:151253-151272 MsG0480018093.01.T01:CDS 45.0%
AGTTGTCCATGTTTGCCGTA+GGG + Chr4:152589-152608 MsG0480018093.01.T01:intron 45.0%
ATATGTCCCTACGGCAAACA+TGG - Chr4:152598-152617 None:intergenic 45.0%
ATCTCTTTCTTCCTATGCCC+TGG - Chr4:151267-151286 None:intergenic 45.0%
ATTAGGCCGGATGTCATGAT+CGG + Chr4:153149-153168 MsG0480018093.01.T01:intron 45.0%
CATACTGCATGGGCTCTTAT+GGG + Chr4:151655-151674 MsG0480018093.01.T01:CDS 45.0%
GCCTTCCACTGTGATTTAAC+TGG + Chr4:152328-152347 MsG0480018093.01.T01:intron 45.0%
GCTAACCAGAAGAATCAGAG+TGG + Chr4:153627-153646 MsG0480018093.01.T01:intron 45.0%
GCTTGACTGCCCAACTTATT+TGG - Chr4:152426-152445 None:intergenic 45.0%
GGATTGAGATGAACCTGTTG+GGG - Chr4:151775-151794 None:intergenic 45.0%
GGTTCATGGTATGGAAACTG+GGG + Chr4:151342-151361 MsG0480018093.01.T01:CDS 45.0%
TACAAACAGCCGATGGTTCA+TGG + Chr4:151328-151347 MsG0480018093.01.T01:CDS 45.0%
TCATGAAGTCGGCGAATGAT+TGG - Chr4:151556-151575 None:intergenic 45.0%
TTTGCGAATAGCAGCGCAAT+TGG - Chr4:151433-151452 None:intergenic 45.0%
! AATTTTGCAGCACGATGGAG+AGG - Chr4:151718-151737 None:intergenic 45.0%
! CACACATAAAGTGGAGGTAC+CGG - Chr4:153185-153204 None:intergenic 45.0%
! CTCTAACCTTGATGATTGGC+AGG + Chr4:153042-153061 MsG0480018093.01.T01:intron 45.0%
! TGAGCAATTTTGCAGCACGA+TGG - Chr4:151723-151742 None:intergenic 45.0%
!! ACACATAAAGTGGAGGTACC+GGG - Chr4:153184-153203 None:intergenic 45.0%
ACAACACAGAGAGAGGATGG+TGG + Chr4:151471-151490 MsG0480018093.01.T01:CDS 50.0%
ACAGCCGATGGTTCATGGTA+TGG + Chr4:151333-151352 MsG0480018093.01.T01:CDS 50.0%
ACCAAGTCAGAAGCTTGGCA+CGG + Chr4:153879-153898 MsG0480018093.01.T01:CDS 50.0%
CAAACCCCGATCATGACATC+CGG - Chr4:153158-153177 None:intergenic 50.0%
CAACACAGAGAGAGGATGGT+GGG + Chr4:151472-151491 MsG0480018093.01.T01:CDS 50.0%
CCAATCGCCTTCTTCCAACT+GGG - Chr4:151752-151771 None:intergenic 50.0%
CCTCTCTCTGTGTTGTGAGA+AGG - Chr4:151467-151486 None:intergenic 50.0%
CCTTCTCACAACACAGAGAG+AGG + Chr4:151464-151483 MsG0480018093.01.T01:CDS 50.0%
CTCACAACACAGAGAGAGGA+TGG + Chr4:151468-151487 MsG0480018093.01.T01:CDS 50.0%
TAACCACTCTTGAGCTCCTG+CGG - Chr4:151050-151069 None:intergenic 50.0%
TGGAACCCGTCTACGATACT+CGG - Chr4:152306-152325 None:intergenic 50.0%
TTAGGCCGGATGTCATGATC+GGG + Chr4:153150-153169 MsG0480018093.01.T01:intron 50.0%
TTCCCACCAAGTCAGAAGCT+TGG + Chr4:153874-153893 MsG0480018093.01.T01:CDS 50.0%
! CTCTAGCCGAGTATCGTAGA+CGG + Chr4:152297-152316 MsG0480018093.01.T01:intron 50.0%
! TCTAGCCGAGTATCGTAGAC+GGG + Chr4:152298-152317 MsG0480018093.01.T01:intron 50.0%
!! CACATAAAGTGGAGGTACCG+GGG - Chr4:153183-153202 None:intergenic 50.0%
!! GGTTCTTGGTTTGCTCTTGG+TGG + Chr4:151381-151400 MsG0480018093.01.T01:CDS 50.0%
!! TGCCAAGCTTCTGACTTGGT+GGG - Chr4:153879-153898 None:intergenic 50.0%
AACCACTCTTGAGCTCCTGC+GGG - Chr4:151049-151068 None:intergenic 55.0%
ATACTCGGCTAGAGCCCACA+AGG - Chr4:152291-152310 None:intergenic 55.0%
CAGCACGATGGAGAGGAGTA+GGG - Chr4:151711-151730 None:intergenic 55.0%
CATGATCGGGGTTTGAACCC+CGG + Chr4:153163-153182 MsG0480018093.01.T01:intron 55.0%
CCCAGTTGGAAGAAGGCGAT+TGG + Chr4:151749-151768 MsG0480018093.01.T01:CDS 55.0%
GCCAATCGCCTTCTTCCAAC+TGG - Chr4:151753-151772 None:intergenic 55.0%
GGGTTTAATTCATGCCGGCC+AGG + Chr4:151675-151694 MsG0480018093.01.T01:CDS 55.0%
TACTCGGCTAGAGCCCACAA+GGG - Chr4:152290-152309 None:intergenic 55.0%
TAGGCCGGATGTCATGATCG+GGG + Chr4:153151-153170 MsG0480018093.01.T01:intron 55.0%
TGGGCTCCTGCCAATCATCA+AGG - Chr4:153051-153070 None:intergenic 55.0%
! CTTGGTGGTTTAGCAGCTGC+TGG + Chr4:151396-151415 MsG0480018093.01.T01:CDS 55.0%
! TTGGATCGGCTTCCTTCGAG+AGG - Chr4:151628-151647 None:intergenic 55.0%
!! ACAGAGAGAGGATGGTGGGT+GGG + Chr4:151476-151495 MsG0480018093.01.T01:CDS 55.0%
!! GTGCCAAGCTTCTGACTTGG+TGG - Chr4:153880-153899 None:intergenic 55.0%
ACTCGGCTAGAGCCCACAAG+GGG - Chr4:152289-152308 None:intergenic 60.0%
GAGTAGGGTCTCTCTCTGCC+TGG - Chr4:151696-151715 None:intergenic 60.0%
GCAGCACGATGGAGAGGAGT+AGG - Chr4:151712-151731 None:intergenic 60.0%
GCCAGTGCCAACATGTGGCA+CGG + Chr4:153901-153920 MsG0480018093.01.T01:CDS 60.0%
GCCGTGCCAAGCTTCTGACT+TGG - Chr4:153883-153902 None:intergenic 60.0%
! AGAAGGCGATTGGCCCCAAC+AGG + Chr4:151759-151778 MsG0480018093.01.T01:CDS 60.0%
!! AGAGAGAGGATGGTGGGTGG+GGG + Chr4:151478-151497 MsG0480018093.01.T01:CDS 60.0%
!! CACAGAGAGAGGATGGTGGG+TGG + Chr4:151475-151494 MsG0480018093.01.T01:CDS 60.0%
!! CAGAGAGAGGATGGTGGGTG+GGG + Chr4:151477-151496 MsG0480018093.01.T01:CDS 60.0%
!! GCAAAAGAGGCTCTGGGAGG+TGG - Chr4:153932-153951 None:intergenic 60.0%
!! GCAGCAAAAGAGGCTCTGGG+AGG - Chr4:153935-153954 None:intergenic 60.0%
AGCCCGCAGGAGCTCAAGAG+TGG + Chr4:151044-151063 MsG0480018093.01.T01:CDS 65.0%
AGGGTCTCTCTCTGCCTGGC+CGG - Chr4:151692-151711 None:intergenic 65.0%
GCACGGCCAGTGCCAACATG+TGG + Chr4:153896-153915 MsG0480018093.01.T01:CDS 65.0%
GCCGTGCCACATGTTGGCAC+TGG - Chr4:153905-153924 None:intergenic 65.0%
!! AGAGGCTCTGGGAGGTGGCA+CGG - Chr4:153927-153946 None:intergenic 65.0%
!! GAGAGAGGATGGTGGGTGGG+GGG + Chr4:151479-151498 MsG0480018093.01.T01:CDS 65.0%
GGCACGGCCGTGCCACATGT+TGG - Chr4:153911-153930 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 151039 154126 151039 ID=MsG0480018093.01;Name=MsG0480018093.01
Chr4 mRNA 151039 154126 151039 ID=MsG0480018093.01.T01;Parent=MsG0480018093.01;Name=MsG0480018093.01.T01;_AED=0.48;_eAED=0.50;_QI=0|0.5|0.6|1|0.75|0.8|5|84|301
Chr4 exon 151039 151065 151039 ID=MsG0480018093.01.T01:exon:428;Parent=MsG0480018093.01.T01
Chr4 exon 151159 151527 151159 ID=MsG0480018093.01.T01:exon:429;Parent=MsG0480018093.01.T01
Chr4 exon 151616 151780 151616 ID=MsG0480018093.01.T01:exon:430;Parent=MsG0480018093.01.T01
Chr4 exon 152224 152306 152224 ID=MsG0480018093.01.T01:exon:431;Parent=MsG0480018093.01.T01
Chr4 exon 153781 154126 153781 ID=MsG0480018093.01.T01:exon:432;Parent=MsG0480018093.01.T01
Chr4 CDS 151039 151065 151039 ID=MsG0480018093.01.T01:cds;Parent=MsG0480018093.01.T01
Chr4 CDS 151159 151527 151159 ID=MsG0480018093.01.T01:cds;Parent=MsG0480018093.01.T01
Chr4 CDS 151616 151780 151616 ID=MsG0480018093.01.T01:cds;Parent=MsG0480018093.01.T01
Chr4 CDS 152224 152306 152224 ID=MsG0480018093.01.T01:cds;Parent=MsG0480018093.01.T01
Chr4 CDS 153781 154042 153781 ID=MsG0480018093.01.T01:cds;Parent=MsG0480018093.01.T01
Chr4 three_prime_UTR 154043 154126 154043 ID=MsG0480018093.01.T01:three_prime_utr;Parent=MsG0480018093.01.T01
Gene Sequence

>MsG0480018093.01.T01

ATGGGAGCCCGCAGGAGCTCAAGAGTGCTACTTAATCCCACTGAGTTTTTTGCGGACATTGTTGTTGAGCATGAATATGTTGAGTGCACTGGATCAGCAATTCAAGCTTTAGTTTTGTTCAAGAAGCTATATCCAGGGCATAGGAAGAAAGAGATAGAGAATTTCATCTCCGAGGCAGTTCGATTCATTGAAGATATACAAACAGCCGATGGTTCATGGTATGGAAACTGGGGAGTTTGTTTCACTTATGGTTCTTGGTTTGCTCTTGGTGGTTTAGCAGCTGCTGGCAAGACTTATACCAATTGCGCTGCTATTCGCAAAGCTGTTAAATTCCTTCTCACAACACAGAGAGAGGATGGTGGGTGGGGGGAGAGCTATCTTTCAAGCCCAAAGAAGATATATGTACCTCTCGAAGGAAGCCGATCCAATGTTGTACATACTGCATGGGCTCTTATGGGTTTAATTCATGCCGGCCAGGCAGAGAGAGACCCTACTCCTCTCCATCGTGCTGCAAAATTGCTCATCAATTCCCAGTTGGAAGAAGGCGATTGGCCCCAACAGGAAATCACAGGAGTATTCATGAAAAATTGTATGTTGCATTACCCAATGTATAGAGATATTTACCCCTTGTGGGCTCTAGCCGAGAACGAATATATGGTGGAAGGTGTCTTGAAGCAAAAGAAAGAGGACTGGAGCAAATACAGTGCGAAAATACGAAGATATGGAACAAAAATATGTTCCCACCAAGTCAGAAGCTTGGCACGGCCAGTGCCAACATGTGGCACGGCCGTGCCACCTCCCAGAGCCTCTTTTGCTGCTTTTTCTTCGGGCCCAAGTTACCCTGTTGCAGCTTTACCGTTTTACCCTTTTAGTTGCTTAGTTTCTAAGTCGATTGTAAAGCTATAA
Protein sequence

>MsG0480018093.01.T01

MGARRSSRVLLNPTEFFADIVVEHEYVECTGSAIQALVLFKKLYPGHRKKEIENFISEAVRFIEDIQTADGSWYGNWGVCFTYGSWFALGGLAAAGKTYTNCAAIRKAVKFLLTTQREDGGWGESYLSSPKKIYVPLEGSRSNVVHTAWALMGLIHAGQAERDPTPLHRAAKLLINSQLEEGDWPQQEITGVFMKNCMLHYPMYRDIYPLWALAENEYMVEGVLKQKKEDWSKYSAKIRRYGTKICSHQVRSLARPVPTCGTAVPPPRASFAAFSSGPSYPVAALPFYPFSCLVSKSIVKL*