AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018207.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018207.01.T01 MTR_4g007410 93.023 43 3 0 38 80 12 54 1.50e-25 92.4
MsG0480018207.01.T01 MTR_7g005900 91.667 36 3 0 45 80 8 43 2.82e-19 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018207.01.T01 AT3G05870 85.455 55 6 1 26 80 1 53 5.51e-29 101
MsG0480018207.01.T01 AT3G05870 85.455 55 6 1 26 80 1 53 5.51e-29 101
MsG0480018207.01.T01 AT3G05870 85.455 55 6 1 26 80 1 53 5.51e-29 101
MsG0480018207.01.T01 AT3G05870 86.957 46 6 0 35 80 11 56 1.64e-25 92.4
MsG0480018207.01.T01 AT3G05870 86.957 46 6 0 35 80 11 56 1.64e-25 92.4
MsG0480018207.01.T01 AT3G05870 86.957 46 6 0 35 80 11 56 1.64e-25 92.4
MsG0480018207.01.T01 AT3G05870 93.023 43 3 0 38 80 2 44 2.32e-25 91.7
MsG0480018207.01.T01 AT5G26640 84.375 32 5 0 45 76 5 36 5.37e-14 62.4

Find 60 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTACAAGTTACAACAATT+AGG 0.258445 4:+1562264 None:intergenic
TTTCCTACTACTTGCAGTTT+GGG 0.267390 4:-1558738 MsG0480018207.01.T01:CDS
ATGCTGTTGCTTCATGGACT+TGG 0.279744 4:-1559609 MsG0480018207.01.T01:CDS
CGTCGGGAATGGCAATTTAA+AGG 0.290239 4:-1558632 None:intergenic
CTTACAAGTTACAACAATTA+GGG 0.315535 4:+1562265 None:intergenic
ATGCACAGGGGTAGTGAGGC+AGG 0.329786 4:-1560234 MsG0480018207.01.T01:intron
ATTTGCATTGTATCTTGAAA+TGG 0.352612 4:-1558694 MsG0480018207.01.T01:CDS
CCTAATTGTTGTAACTTGTA+AGG 0.390094 4:-1562264 MsG0480018207.01.T01:intron
GTGGTCGCGTTGCTTGGTCT+TGG 0.395537 4:+1562419 None:intergenic
TGTCCTGATTGTAAACTTCC+CGG 0.396496 4:-1559535 MsG0480018207.01.T01:CDS
GATGGCATGCTGTTGCTTCA+TGG 0.397141 4:-1559615 MsG0480018207.01.T01:CDS
GCCATTCCCGACGGCACATT+GGG 0.397444 4:+1558642 None:intergenic
ATTTGTAGGATGGCCTTTGA+TGG 0.411828 4:-1559562 MsG0480018207.01.T01:CDS
TGCTGTTGCTTCATGGACTT+GGG 0.419431 4:-1559608 MsG0480018207.01.T01:CDS
TCCTCCTCCATCTTCTGCAT+TGG 0.420963 4:-1562328 MsG0480018207.01.T01:five_prime_UTR
ATGAAACATGTGGGATTTGT+AGG 0.429235 4:-1559576 MsG0480018207.01.T01:CDS
GGAGGAGGATGCGGCGGTGA+TGG 0.440552 4:+1562342 None:intergenic
GTTACAACAATTAGGGTTTG+TGG 0.445339 4:+1562272 None:intergenic
TGCCATTCCCGACGGCACAT+TGG 0.451421 4:+1558641 None:intergenic
GAGGATGCGGCGGTGATGGA+TGG 0.452516 4:+1562346 None:intergenic
GTCGTGTGGTGGTGGATGGA+TGG 0.452673 4:+1562393 None:intergenic
TGGAGAGTGGTCGCGTTGCT+TGG 0.459797 4:+1562413 None:intergenic
ACTGAGCGGGCAGTCATCCC+CGG 0.466023 4:+1559516 None:intergenic
TGAAGAACCAATGCAGAAGA+TGG 0.471925 4:+1562321 None:intergenic
GTCCTGATTGTAAACTTCCC+GGG 0.472599 4:-1559534 MsG0480018207.01.T01:CDS
GCCCAATGTGCCGTCGGGAA+TGG 0.481653 4:-1558643 MsG0480018207.01.T01:CDS
TTTGCATTGTATCTTGAAAT+GGG 0.482104 4:-1558693 MsG0480018207.01.T01:CDS
CTATTAACCTTTGAAGGACG+AGG 0.493544 4:+1561595 None:intergenic
GCAGAAGATGGAGGAGGATG+CGG 0.504457 4:+1562333 None:intergenic
GGCGGTCGTGTGGTGGTGGA+TGG 0.505870 4:+1562389 None:intergenic
TCGCGGCGGTCGTGTGGTGG+TGG 0.510950 4:+1562385 None:intergenic
CACATTGCCCAATGTGCCGT+CGG 0.515957 4:-1558649 MsG0480018207.01.T01:CDS
TCCCCGGGAAGTTTACAATC+AGG 0.518100 4:+1559532 None:intergenic
CACCCCAAACTGCAAGTAGT+AGG 0.519490 4:+1558735 None:intergenic
GCACAGGGGTAGTGAGGCAG+GGG 0.527058 4:-1560232 MsG0480018207.01.T01:intron
TTCCTACTACTTGCAGTTTG+GGG 0.533411 4:-1558737 MsG0480018207.01.T01:CDS
CTTTAAATTGCCATTCCCGA+CGG 0.544857 4:+1558633 None:intergenic
AAATGAACCTCGTCCTTCAA+AGG 0.555109 4:-1561602 MsG0480018207.01.T01:five_prime_UTR
TGCACAGGGGTAGTGAGGCA+GGG 0.559698 4:-1560233 MsG0480018207.01.T01:intron
ACATTGCCCAATGTGCCGTC+GGG 0.562769 4:-1558648 MsG0480018207.01.T01:CDS
GAGTGATCGCGGCGGTCGTG+TGG 0.564527 4:+1562379 None:intergenic
CTGAGCGGGCAGTCATCCCC+GGG 0.565342 4:+1559517 None:intergenic
GCGGCGGTGATGGATGGAGA+AGG 0.567174 4:+1562352 None:intergenic
TCCTGATTGTAAACTTCCCG+GGG 0.576107 4:-1559533 MsG0480018207.01.T01:CDS
TTAGCAGTAGCTTACTGAGC+GGG 0.577553 4:+1559503 None:intergenic
GGTGGTGGATGGATGGAGAG+TGG 0.581683 4:+1562400 None:intergenic
ACCAATGCAGAAGATGGAGG+AGG 0.595744 4:+1562327 None:intergenic
TGATCGCGGCGGTCGTGTGG+TGG 0.596215 4:+1562382 None:intergenic
GCGGTGATGGATGGAGAAGG+AGG 0.610844 4:+1562355 None:intergenic
TGTCATGCACAGGGGTAGTG+AGG 0.620052 4:-1560238 MsG0480018207.01.T01:intron
ATCAGGACAACATCCATCAA+AGG 0.626104 4:+1559549 None:intergenic
GAAGATGGAGGAGGATGCGG+CGG 0.630764 4:+1562336 None:intergenic
AGAACCAATGCAGAAGATGG+AGG 0.632022 4:+1562324 None:intergenic
GAGAAGGAGGAGAGTGATCG+CGG 0.648912 4:+1562368 None:intergenic
ATCTCTGTGATGTGAATCAG+TGG 0.650177 4:+1561515 None:intergenic
AACATGTGGGATTTGTAGGA+TGG 0.654714 4:-1559572 MsG0480018207.01.T01:CDS
GATGCACAAGATGAAACATG+TGG 0.720145 4:-1559586 MsG0480018207.01.T01:CDS
ATGCACAAGATGAAACATGT+GGG 0.725526 4:-1559585 MsG0480018207.01.T01:CDS
TCTGTGATGTGAATCAGTGG+CGG 0.733896 4:+1561518 None:intergenic
AAGGAGGAGAGTGATCGCGG+CGG 0.760184 4:+1562371 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTGAATGTAATAATTT+GGG - Chr4:1559002-1559021 MsG0480018207.01.T01:intron 10.0%
!! TTTAATTGAATGTAATAATT+TGG - Chr4:1559001-1559020 MsG0480018207.01.T01:intron 10.0%
!!! AACATTTTTTTTTTTTGAAA+TGG + Chr4:1561050-1561069 None:intergenic 10.0%
!!! ATTGTAAGTTTTTATTTTTT+TGG - Chr4:1559199-1559218 MsG0480018207.01.T01:intron 10.0%
!!! ATTTTAATCAAAATTTTTGT+TGG - Chr4:1561343-1561362 MsG0480018207.01.T01:intron 10.0%
!!! TTCAATTTTTTTTATGAATT+GGG - Chr4:1559728-1559747 MsG0480018207.01.T01:intron 10.0%
!!! TTTCAATTTTTTTTATGAAT+TGG - Chr4:1559727-1559746 MsG0480018207.01.T01:intron 10.0%
!!! TTTTAATCAAAATTTTTGTT+GGG - Chr4:1561344-1561363 MsG0480018207.01.T01:intron 10.0%
!! AATCATAAAAAAAGAAATGT+CGG + Chr4:1560471-1560490 None:intergenic 15.0%
!! ATAAATAAGAAAGTAATCAA+GGG - Chr4:1560299-1560318 MsG0480018207.01.T01:intron 15.0%
!! ATATTTATTGAGTATATGTT+TGG - Chr4:1560114-1560133 MsG0480018207.01.T01:intron 15.0%
!! CTAATTATATGTAATAACAA+AGG - Chr4:1561287-1561306 MsG0480018207.01.T01:intron 15.0%
!! GAAATTAAAAAGATAGAAAA+TGG + Chr4:1560980-1560999 None:intergenic 15.0%
!! GTAGTTTCATAAATATAATT+AGG - Chr4:1561979-1561998 MsG0480018207.01.T01:intron 15.0%
!! TATAAATAAGAAAGTAATCA+AGG - Chr4:1560298-1560317 MsG0480018207.01.T01:intron 15.0%
!! TGAGGAATAATATAATTTAA+GGG - Chr4:1560366-1560385 MsG0480018207.01.T01:intron 15.0%
!! ACATATAATTAGTGTGTATT+TGG + Chr4:1561279-1561298 None:intergenic 20.0%
!! ATATGTATTTAAGCATGTAT+GGG + Chr4:1559940-1559959 None:intergenic 20.0%
!! GTGAGGAATAATATAATTTA+AGG - Chr4:1560365-1560384 MsG0480018207.01.T01:intron 20.0%
!! TAAATAAGAAAGTAATCAAG+GGG - Chr4:1560300-1560319 MsG0480018207.01.T01:intron 20.0%
!! TATTTGCATAAATTTGTCTT+TGG - Chr4:1561623-1561642 MsG0480018207.01.T01:intron 20.0%
!! TTATATGTTAAGAATGAAGA+GGG - Chr4:1559166-1559185 MsG0480018207.01.T01:intron 20.0%
!! TTCAATTAAAGAACAACAAA+TGG + Chr4:1558974-1558993 None:intergenic 20.0%
!! TTTATATGTTAAGAATGAAG+AGG - Chr4:1559165-1559184 MsG0480018207.01.T01:intron 20.0%
!!! AATTTTGTTTTTCTTGACTT+AGG - Chr4:1559768-1559787 MsG0480018207.01.T01:intron 20.0%
!!! ACATTTTTATTGAAATCAAG+TGG - Chr4:1560035-1560054 MsG0480018207.01.T01:intron 20.0%
!!! ACTGAAAATTTGAAATTACT+AGG + Chr4:1561370-1561389 None:intergenic 20.0%
!!! ATTGTATGTTTTTGTTACAA+TGG - Chr4:1559883-1559902 MsG0480018207.01.T01:intron 20.0%
!!! CATTTTTATTGAAATCAAGT+GGG - Chr4:1560036-1560055 MsG0480018207.01.T01:intron 20.0%
!!! GACATTTCTTTTTTTATGAT+TGG - Chr4:1560470-1560489 MsG0480018207.01.T01:intron 20.0%
!!! GTAATTGACAATTCTATTTT+GGG - Chr4:1562125-1562144 MsG0480018207.01.T01:intron 20.0%
!!! TCAGTTCATTTCTAATTTTT+CGG - Chr4:1561920-1561939 MsG0480018207.01.T01:intron 20.0%
!!! TTATAAAAGCTTTATTCTCA+AGG - Chr4:1562049-1562068 MsG0480018207.01.T01:intron 20.0%
!!! TTGTTCTGTAAAAATGATTT+TGG + Chr4:1561240-1561259 None:intergenic 20.0%
! AAACTACAAATGATGTTTAG+AGG + Chr4:1561966-1561985 None:intergenic 25.0%
! AAAGCAAAAATCAATTCTAG+AGG + Chr4:1561093-1561112 None:intergenic 25.0%
! AATAAATTTGGCATAAACTG+TGG + Chr4:1559119-1559138 None:intergenic 25.0%
! AATTGTCAATTACCATCTTA+TGG + Chr4:1562118-1562137 None:intergenic 25.0%
! AGAATCATATTTGATCAGTT+AGG + Chr4:1562277-1562296 None:intergenic 25.0%
! AGATGTTTCAATGCAATAAT+TGG + Chr4:1559037-1559056 None:intergenic 25.0%
! AGGAATTGAAGAATAATGAA+AGG + Chr4:1559624-1559643 None:intergenic 25.0%
! ATGTAATAACAAAGGTTGTA+TGG - Chr4:1561295-1561314 MsG0480018207.01.T01:intron 25.0%
! ATTTGCATTGTATCTTGAAA+TGG - Chr4:1562378-1562397 MsG0480018207.01.T01:five_prime_UTR 25.0%
! CTTACAAGTTACAACAATTA+GGG + Chr4:1558810-1558829 None:intergenic 25.0%
! GATATGTATTTAAGCATGTA+TGG + Chr4:1559941-1559960 None:intergenic 25.0%
! GGAATTGAAGAATAATGAAA+GGG + Chr4:1559623-1559642 None:intergenic 25.0%
! TAAAAACTTACAATTCATCC+TGG + Chr4:1559193-1559212 None:intergenic 25.0%
! TCTTTCTATACTTCTGATAA+TGG - Chr4:1559335-1559354 MsG0480018207.01.T01:intron 25.0%
! TTAATTGTTCTCTTGTGATT+TGG - Chr4:1560175-1560194 MsG0480018207.01.T01:intron 25.0%
! TTTGCATTGTATCTTGAAAT+GGG - Chr4:1562379-1562398 MsG0480018207.01.T01:five_prime_UTR 25.0%
!! AACTAAAAGTCTCTTTTCTA+AGG + Chr4:1561790-1561809 None:intergenic 25.0%
!! AAGGTAAAGTGATTTTTGTT+TGG + Chr4:1561203-1561222 None:intergenic 25.0%
!! AATAGTTGCAAAGATGTTTT+AGG - Chr4:1559985-1560004 MsG0480018207.01.T01:intron 25.0%
!! ATAGTTGCAAAGATGTTTTA+GGG - Chr4:1559986-1560005 MsG0480018207.01.T01:intron 25.0%
!! ATGAACTTGAACAATTTTGA+TGG - Chr4:1559503-1559522 MsG0480018207.01.T01:intron 25.0%
!! ATTTAGGATTAGGATTACAA+TGG - Chr4:1559914-1559933 MsG0480018207.01.T01:intron 25.0%
!! CATTTTCTATTAACCTTTGA+AGG + Chr4:1559486-1559505 None:intergenic 25.0%
!! CTGCATAATTGAGAATTTTT+CGG - Chr4:1562087-1562106 MsG0480018207.01.T01:intron 25.0%
!! GGTAATTGACAATTCTATTT+TGG - Chr4:1562124-1562143 MsG0480018207.01.T01:intron 25.0%
!!! CTTTTTTTATGATTGGTGTT+GGG - Chr4:1560477-1560496 MsG0480018207.01.T01:intron 25.0%
!!! GGTTTTTTGTGTTAGTTTTT+TGG - Chr4:1560921-1560940 MsG0480018207.01.T01:intron 25.0%
!!! TAATTTTTCGGAATGTAGAT+AGG - Chr4:1561932-1561951 MsG0480018207.01.T01:intron 25.0%
!!! TCTTTTTTTATGATTGGTGT+TGG - Chr4:1560476-1560495 MsG0480018207.01.T01:intron 25.0%
AACAACAGATGGAAAAATCA+TGG + Chr4:1558909-1558928 None:intergenic 30.0%
AGGGAATTGAAATATTGAGA+AGG + Chr4:1559604-1559623 None:intergenic 30.0%
AGGGTAATAATCAATTCTCA+AGG + Chr4:1561138-1561157 None:intergenic 30.0%
CCTTACAAGTTACAACAATT+AGG + Chr4:1558811-1558830 None:intergenic 30.0%
CGAAAAATTCTCAATTATGC+AGG + Chr4:1562089-1562108 None:intergenic 30.0%
CTACAAAGATGTATTGTTAC+TGG - Chr4:1562010-1562029 MsG0480018207.01.T01:intron 30.0%
GAAATGAATGATAAATCCCA+GGG + Chr4:1561157-1561176 None:intergenic 30.0%
GGTAATATGCAAAATGAAAG+AGG + Chr4:1559644-1559663 None:intergenic 30.0%
GTAAAGCTTAACTTCATAAC+TGG - Chr4:1561696-1561715 MsG0480018207.01.T01:intron 30.0%
GTCCAGAATCAGAATAAATT+TGG + Chr4:1559131-1559150 None:intergenic 30.0%
TACAATGGAATGTCTGATTT+AGG - Chr4:1559898-1559917 MsG0480018207.01.T01:intron 30.0%
TATGAAACAAGATACCTTAC+TGG + Chr4:1559387-1559406 None:intergenic 30.0%
TATTCTGATTCTGGACTAAT+TGG - Chr4:1559135-1559154 MsG0480018207.01.T01:intron 30.0%
TCAAGGGGTTATGATTAATT+TGG - Chr4:1560315-1560334 MsG0480018207.01.T01:intron 30.0%
TGAAATGAATGATAAATCCC+AGG + Chr4:1561158-1561177 None:intergenic 30.0%
TGAGAATTGATTATTACCCT+GGG - Chr4:1561138-1561157 MsG0480018207.01.T01:intron 30.0%
TGCCAAATTTATTCTGATTC+TGG - Chr4:1559126-1559145 MsG0480018207.01.T01:intron 30.0%
TTATGAGGTAGATGAAATTG+TGG - Chr4:1560395-1560414 MsG0480018207.01.T01:intron 30.0%
TTGAGAATTGATTATTACCC+TGG - Chr4:1561137-1561156 MsG0480018207.01.T01:intron 30.0%
TTTAGGGATTGATACAACAT+TGG - Chr4:1560002-1560021 MsG0480018207.01.T01:intron 30.0%
TTTATTGAAATCAAGTGGGA+AGG - Chr4:1560040-1560059 MsG0480018207.01.T01:intron 30.0%
! AATTTGGCTCACAACAATTT+GGG - Chr4:1560331-1560350 MsG0480018207.01.T01:intron 30.0%
! CCTAATTGTTGTAACTTGTA+AGG - Chr4:1558808-1558827 MsG0480018207.01.T01:intron 30.0%
! GCATAATTTGTCTTTCTCTA+TGG - Chr4:1562227-1562246 MsG0480018207.01.T01:intron 30.0%
! TAATTTGGCTCACAACAATT+TGG - Chr4:1560330-1560349 MsG0480018207.01.T01:intron 30.0%
! TGTAATCTTAGATTGGTTCA+AGG - Chr4:1559280-1559299 MsG0480018207.01.T01:intron 30.0%
!! AACTTGAACAATTTTGATGG+CGG - Chr4:1559506-1559525 MsG0480018207.01.T01:intron 30.0%
!! AAGCTTTATTCTCAAGGAAT+TGG - Chr4:1562055-1562074 MsG0480018207.01.T01:intron 30.0%
!! ATTTAAGGGTGTCAATTATG+AGG - Chr4:1560380-1560399 MsG0480018207.01.T01:intron 30.0%
!! TATATGTTTGGTTCCACTTT+TGG - Chr4:1560126-1560145 MsG0480018207.01.T01:intron 30.0%
!! TCACAACAATTTGGGTTTTA+TGG - Chr4:1560339-1560358 MsG0480018207.01.T01:intron 30.0%
!! TCTATACTTCTGATAATGGT+AGG - Chr4:1559339-1559358 MsG0480018207.01.T01:intron 30.0%
!!! ACAACTTCTGTTTTTGTGTT+AGG - Chr4:1560638-1560657 MsG0480018207.01.T01:intron 30.0%
AAATTGTACTTCCTCTTGCT+TGG - Chr4:1560678-1560697 MsG0480018207.01.T01:intron 35.0%
AAGATACCTTACTGGATTTG+TGG + Chr4:1559379-1559398 None:intergenic 35.0%
AATTATGCCTTGGGAGATAA+GGG - Chr4:1560512-1560531 MsG0480018207.01.T01:intron 35.0%
AATTGGGTATTTGCAGAACA+TGG - Chr4:1559744-1559763 MsG0480018207.01.T01:intron 35.0%
ATGAAACATGTGGGATTTGT+AGG - Chr4:1561496-1561515 MsG0480018207.01.T01:intron 35.0%
ATGCACAAGATGAAACATGT+GGG - Chr4:1561487-1561506 MsG0480018207.01.T01:intron 35.0%
CATAGCGAGACATGATTAAA+CGG + Chr4:1559963-1559982 None:intergenic 35.0%
GGACTTGTGTAATCTTAGAT+TGG - Chr4:1559273-1559292 MsG0480018207.01.T01:intron 35.0%
GTTACAACAATTAGGGTTTG+TGG + Chr4:1558803-1558822 None:intergenic 35.0%
TCCTAACCTCTTTGTTCATT+GGG - Chr4:1560256-1560275 MsG0480018207.01.T01:intron 35.0%
TCTCTTACGTCAAATGTAGA+TGG - Chr4:1561439-1561458 MsG0480018207.01.T01:intron 35.0%
TGTGAAGCAAAAGAACAAAC+AGG + Chr4:1559665-1559684 None:intergenic 35.0%
TTTCCTACTACTTGCAGTTT+GGG - Chr4:1562334-1562353 MsG0480018207.01.T01:five_prime_UTR 35.0%
! AATTTTGCTTGCTCCAAAAG+TGG + Chr4:1560142-1560161 None:intergenic 35.0%
! GGAATGTCTGATTTAGGATT+AGG - Chr4:1559904-1559923 MsG0480018207.01.T01:intron 35.0%
! GTTTTTGTGTTAGGAACAGA+TGG - Chr4:1560647-1560666 MsG0480018207.01.T01:intron 35.0%
! TGACTTAGGTTTCTCCTTTT+GGG - Chr4:1559782-1559801 MsG0480018207.01.T01:intron 35.0%
! TGATGTCTGTCTTTGTTTCT+CGG - Chr4:1562185-1562204 MsG0480018207.01.T01:intron 35.0%
! TTGACTTAGGTTTCTCCTTT+TGG - Chr4:1559781-1559800 MsG0480018207.01.T01:intron 35.0%
! TTTGGCTAAAAGTGAGTTGA+AGG + Chr4:1561222-1561241 None:intergenic 35.0%
! TTTTCCATCTGTTGTTAAGC+CGG - Chr4:1558913-1558932 MsG0480018207.01.T01:intron 35.0%
! TTTTCCTACTACTTGCAGTT+TGG - Chr4:1562333-1562352 MsG0480018207.01.T01:five_prime_UTR 35.0%
!! TTTATGATTGGTGTTGGGAT+TGG - Chr4:1560482-1560501 MsG0480018207.01.T01:intron 35.0%
!! TTTGGGTTTTATGGAAAGTG+AGG - Chr4:1560348-1560367 MsG0480018207.01.T01:intron 35.0%
!!! AGTCATCTTTGTTTTGCCTT+TGG - Chr4:1559070-1559089 MsG0480018207.01.T01:intron 35.0%
!!! AGTTTTTTGGTGTTGTGACT+GGG - Chr4:1560934-1560953 MsG0480018207.01.T01:intron 35.0%
!!! CACTGTAGTTTTTTAGTGGA+GGG - Chr4:1560798-1560817 MsG0480018207.01.T01:intron 35.0%
!!! TAGTTTTTTGGTGTTGTGAC+TGG - Chr4:1560933-1560952 MsG0480018207.01.T01:intron 35.0%
!!! TTTTGGTTTGCTGTGATGTT+TGG - Chr4:1560762-1560781 MsG0480018207.01.T01:intron 35.0%
!!! TTTTTGAGTTCTCTACATGC+AGG - Chr4:1561885-1561904 MsG0480018207.01.T01:intron 35.0%
AAACAGAGCTGCTAAAACAG+CGG + Chr4:1560549-1560568 None:intergenic 40.0%
AAATGAACCTCGTCCTTCAA+AGG - Chr4:1559470-1559489 MsG0480018207.01.T01:intron 40.0%
AACACTCCCAATGAACAAAG+AGG + Chr4:1560265-1560284 None:intergenic 40.0%
AACATGTGGGATTTGTAGGA+TGG - Chr4:1561500-1561519 MsG0480018207.01.T01:CDS 40.0%
AGATCAGCAGATATCCCAAA+AGG + Chr4:1559799-1559818 None:intergenic 40.0%
ATCAGGACAACATCCATCAA+AGG + Chr4:1561526-1561545 None:intergenic 40.0%
ATCTCCCAAGGCATAATTGA+GGG + Chr4:1560510-1560529 None:intergenic 40.0%
ATCTCTGTGATGTGAATCAG+TGG + Chr4:1559560-1559579 None:intergenic 40.0%
ATTAGCAGTAGCTTACTGAG+CGG + Chr4:1561573-1561592 None:intergenic 40.0%
ATTTGTAGGATGGCCTTTGA+TGG - Chr4:1561510-1561529 MsG0480018207.01.T01:CDS 40.0%
CAATGAACAAAGAGGTTAGG+AGG + Chr4:1560257-1560276 None:intergenic 40.0%
CAATTATGCCTTGGGAGATA+AGG - Chr4:1560511-1560530 MsG0480018207.01.T01:intron 40.0%
CCTCCACTAAAAAACTACAG+TGG + Chr4:1560800-1560819 None:intergenic 40.0%
CTCCTAACCTCTTTGTTCAT+TGG - Chr4:1560255-1560274 MsG0480018207.01.T01:intron 40.0%
GAAATCAAGTGGGAAGGAAT+TGG - Chr4:1560046-1560065 MsG0480018207.01.T01:intron 40.0%
GACTGTTAGCACGTATAGTT+CGG - Chr4:1560870-1560889 MsG0480018207.01.T01:intron 40.0%
GAGCCAGACAAAAAAGTTTG+GGG + Chr4:1559535-1559554 None:intergenic 40.0%
GATGCACAAGATGAAACATG+TGG - Chr4:1561486-1561505 MsG0480018207.01.T01:intron 40.0%
GCTACTGCTAATGTTGTCTT+TGG - Chr4:1561581-1561600 MsG0480018207.01.T01:exon 40.0%
GGAGCCAGACAAAAAAGTTT+GGG + Chr4:1559536-1559555 None:intergenic 40.0%
GTAGTGTAGAAGCTCTTTCA+TGG - Chr4:1559252-1559271 MsG0480018207.01.T01:intron 40.0%
GTTGTATGGCTAGTGTGAAT+AGG - Chr4:1561309-1561328 MsG0480018207.01.T01:intron 40.0%
TATCTCCCAAGGCATAATTG+AGG + Chr4:1560511-1560530 None:intergenic 40.0%
TCCCAATGAACAAAGAGGTT+AGG + Chr4:1560260-1560279 None:intergenic 40.0%
TGAAATTGTGGAGGAGATCA+AGG - Chr4:1560407-1560426 MsG0480018207.01.T01:intron 40.0%
TGAAGAACCAATGCAGAAGA+TGG + Chr4:1558754-1558773 None:intergenic 40.0%
TGAGGTAGATGAAATTGTGG+AGG - Chr4:1560398-1560417 MsG0480018207.01.T01:intron 40.0%
TGGAACTGGAGAACAAACAT+GGG - Chr4:1560069-1560088 MsG0480018207.01.T01:intron 40.0%
TGTATTGTTACTGGCATGCA+AGG - Chr4:1562019-1562038 MsG0480018207.01.T01:intron 40.0%
TGTCCTGATTGTAAACTTCC+CGG - Chr4:1561537-1561556 MsG0480018207.01.T01:CDS 40.0%
TGTTTGCCACAAATCCAGTA+AGG - Chr4:1559370-1559389 MsG0480018207.01.T01:intron 40.0%
TTCCTACTACTTGCAGTTTG+GGG - Chr4:1562335-1562354 MsG0480018207.01.T01:five_prime_UTR 40.0%
TTGAGAATTATTCGTCACCG+CGG - Chr4:1562162-1562181 MsG0480018207.01.T01:intron 40.0%
TTTCCATCTGTTGTTAAGCC+GGG - Chr4:1558914-1558933 MsG0480018207.01.T01:intron 40.0%
TTTCGGAATGTAGATAGGTC+TGG - Chr4:1561937-1561956 MsG0480018207.01.T01:intron 40.0%
! CTATTAACCTTTGAAGGACG+AGG + Chr4:1559480-1559499 None:intergenic 40.0%
! TACTTGATTGCGAGGTTTTG+TGG - Chr4:1560899-1560918 MsG0480018207.01.T01:intron 40.0%
! TCTTTGTTCATTGGGAGTGT+TGG - Chr4:1560264-1560283 MsG0480018207.01.T01:intron 40.0%
! TTTTCGGAAGAGCCATAAGA+TGG - Chr4:1562103-1562122 MsG0480018207.01.T01:intron 40.0%
!! ACTTGATTGCGAGGTTTTGT+GGG - Chr4:1560900-1560919 MsG0480018207.01.T01:intron 40.0%
!! CTTCCTCTTGCTTGGTTTTT+AGG - Chr4:1560686-1560705 MsG0480018207.01.T01:intron 40.0%
!! CTTTGTTCATTGGGAGTGTT+GGG - Chr4:1560265-1560284 MsG0480018207.01.T01:intron 40.0%
!! GGTTTTTAGGCTCTTTTGCT+GGG - Chr4:1560699-1560718 MsG0480018207.01.T01:intron 40.0%
!! TGGTTTTTAGGCTCTTTTGC+TGG - Chr4:1560698-1560717 MsG0480018207.01.T01:intron 40.0%
!! TTTGTTCATTGGGAGTGTTG+GGG - Chr4:1560266-1560285 MsG0480018207.01.T01:intron 40.0%
!!! CCACTGTAGTTTTTTAGTGG+AGG - Chr4:1560797-1560816 MsG0480018207.01.T01:intron 40.0%
!!! GCTGTTGGTGTGTTTTTTGA+AGG - Chr4:1560614-1560633 MsG0480018207.01.T01:intron 40.0%
!!! GTGCCACTGTAGTTTTTTAG+TGG - Chr4:1560794-1560813 MsG0480018207.01.T01:intron 40.0%
AAAATGGCGCCGCATATAGA+CGG + Chr4:1560964-1560983 None:intergenic 45.0%
AACAAAGACAGACATCACCG+CGG + Chr4:1562182-1562201 None:intergenic 45.0%
AACCTCGCAATCAAGTAACG+AGG + Chr4:1560896-1560915 None:intergenic 45.0%
ACGTCACACTACTGAACCAA+AGG + Chr4:1559089-1559108 None:intergenic 45.0%
AGAACCAATGCAGAAGATGG+AGG + Chr4:1558751-1558770 None:intergenic 45.0%
ATCAAGTGGGAAGGAATTGG+TGG - Chr4:1560049-1560068 MsG0480018207.01.T01:intron 45.0%
CCAGACAAAAAAGTTTGGGG+TGG + Chr4:1559532-1559551 None:intergenic 45.0%
CGGAGCCAGACAAAAAAGTT+TGG + Chr4:1559537-1559556 None:intergenic 45.0%
GAGCCTAAAAACCAAGCAAG+AGG + Chr4:1560692-1560711 None:intergenic 45.0%
GCAAGATGCAAAAACACTCG+CGG + Chr4:1561002-1561021 None:intergenic 45.0%
GGAACCCTCAATTATGCCTT+GGG - Chr4:1560503-1560522 MsG0480018207.01.T01:intron 45.0%
GTCCTGATTGTAAACTTCCC+GGG - Chr4:1561538-1561557 MsG0480018207.01.T01:CDS 45.0%
GTGGAACTGGAGAACAAACA+TGG - Chr4:1560068-1560087 MsG0480018207.01.T01:intron 45.0%
GTTAAGAATGAAGAGGGTCC+AGG - Chr4:1559172-1559191 MsG0480018207.01.T01:intron 45.0%
TCCTGATTGTAAACTTCCCG+GGG - Chr4:1561539-1561558 MsG0480018207.01.T01:CDS 45.0%
TCTGTGATGTGAATCAGTGG+CGG + Chr4:1559557-1559576 None:intergenic 45.0%
TGAACAAAGAGGTTAGGAGG+AGG + Chr4:1560254-1560273 None:intergenic 45.0%
TGGAAACAAGACAAACCCAC+CGG + Chr4:1558954-1558973 None:intergenic 45.0%
TGGAACCCTCAATTATGCCT+TGG - Chr4:1560502-1560521 MsG0480018207.01.T01:intron 45.0%
TGTGGACGAAATTGATGTGG+TGG - Chr4:1560207-1560226 MsG0480018207.01.T01:intron 45.0%
TTAGCAGTAGCTTACTGAGC+GGG + Chr4:1561572-1561591 None:intergenic 45.0%
TTGTTCTGCTGTCATGCACA+GGG - Chr4:1560825-1560844 MsG0480018207.01.T01:intron 45.0%
TTTGTTCTGCTGTCATGCAC+AGG - Chr4:1560824-1560843 MsG0480018207.01.T01:intron 45.0%
! ATGCTGTTGCTTCATGGACT+TGG - Chr4:1561463-1561482 MsG0480018207.01.T01:intron 45.0%
! CTCTTGTGATTTGGCGTGTT+CGG - Chr4:1560184-1560203 MsG0480018207.01.T01:intron 45.0%
! TGCTGTTGCTTCATGGACTT+GGG - Chr4:1561464-1561483 MsG0480018207.01.T01:intron 45.0%
!! TGGTTTGCTGTGATGTTTGG+AGG - Chr4:1560765-1560784 MsG0480018207.01.T01:intron 45.0%
!!! CCACCCCAAACTTTTTTGTC+TGG - Chr4:1559529-1559548 MsG0480018207.01.T01:CDS 45.0%
ACCAATGCAGAAGATGGAGG+AGG + Chr4:1558748-1558767 None:intergenic 50.0%
ATCTGTTGTTAAGCCGGGTG+AGG - Chr4:1558919-1558938 MsG0480018207.01.T01:intron 50.0%
CACCCCAAACTGCAAGTAGT+AGG + Chr4:1562340-1562359 None:intergenic 50.0%
CGGTGTGGACGAAATTGATG+TGG - Chr4:1560204-1560223 MsG0480018207.01.T01:intron 50.0%
CTGGGTGTTCCGTCTATATG+CGG - Chr4:1560952-1560971 MsG0480018207.01.T01:intron 50.0%
GACGAAATTGATGTGGTGGC+TGG - Chr4:1560211-1560230 MsG0480018207.01.T01:intron 50.0%
TATGCCTTGGGAGATAAGGG+AGG - Chr4:1560515-1560534 MsG0480018207.01.T01:intron 50.0%
TCACCCGGCTTAACAACAGA+TGG + Chr4:1558920-1558939 None:intergenic 50.0%
TCCCCGGGAAGTTTACAATC+AGG + Chr4:1561543-1561562 None:intergenic 50.0%
TCCTCCTCCATCTTCTGCAT+TGG - Chr4:1558744-1558763 MsG0480018207.01.T01:CDS 50.0%
TCTGTTGTTAAGCCGGGTGA+GGG - Chr4:1558920-1558939 MsG0480018207.01.T01:intron 50.0%
TGGGAAGGAATTGGTGGAAC+TGG - Chr4:1560055-1560074 MsG0480018207.01.T01:intron 50.0%
TGTTCTGCTGTCATGCACAG+GGG - Chr4:1560826-1560845 MsG0480018207.01.T01:intron 50.0%
! GATGGCATGCTGTTGCTTCA+TGG - Chr4:1561457-1561476 MsG0480018207.01.T01:intron 50.0%
!! AGGAGATCAAGGTGTTGTCG+TGG - Chr4:1560418-1560437 MsG0480018207.01.T01:intron 50.0%
!! CTGGGTTGATGTTCAGCTGT+TGG - Chr4:1560599-1560618 MsG0480018207.01.T01:intron 50.0%
!! TTCATTGGGAGTGTTGGGGT+GGG - Chr4:1560270-1560289 MsG0480018207.01.T01:intron 50.0%
ACATTGCCCAATGTGCCGTC+GGG - Chr4:1562424-1562443 MsG0480018207.01.T01:five_prime_UTR 55.0%
CACACCTCCCTTATCTCCCA+AGG + Chr4:1560522-1560541 None:intergenic 55.0%
CACATTGCCCAATGTGCCGT+CGG - Chr4:1562423-1562442 MsG0480018207.01.T01:five_prime_UTR 55.0%
CTGTTGTTAAGCCGGGTGAG+GGG - Chr4:1558921-1558940 MsG0480018207.01.T01:intron 55.0%
GAGAAGGAGGAGAGTGATCG+CGG + Chr4:1558707-1558726 None:intergenic 55.0%
GCAGAAGATGGAGGAGGATG+CGG + Chr4:1558742-1558761 None:intergenic 55.0%
GGCCTCGTTACTTGATTGCG+AGG - Chr4:1560891-1560910 MsG0480018207.01.T01:intron 55.0%
TGTCATGCACAGGGGTAGTG+AGG - Chr4:1560834-1560853 MsG0480018207.01.T01:intron 55.0%
TGTTGTTAAGCCGGGTGAGG+GGG - Chr4:1558922-1558941 MsG0480018207.01.T01:intron 55.0%
TGTTTCGCAGCTCTGTTGGC+TGG - Chr4:1560573-1560592 MsG0480018207.01.T01:intron 55.0%
! AGTTGCTGTTGCGGTTGGTG+GGG - Chr4:1560734-1560753 MsG0480018207.01.T01:intron 55.0%
! CAGCTGCTGAGTTGCTGTTG+CGG - Chr4:1560725-1560744 MsG0480018207.01.T01:intron 55.0%
! GAGTTGCTGTTGCGGTTGGT+GGG - Chr4:1560733-1560752 MsG0480018207.01.T01:intron 55.0%
! GTGATTTGGCGTGTTCGGTG+TGG - Chr4:1560189-1560208 MsG0480018207.01.T01:intron 55.0%
! TGAGTTGCTGTTGCGGTTGG+TGG - Chr4:1560732-1560751 MsG0480018207.01.T01:intron 55.0%
! TGCTGAGTTGCTGTTGCGGT+TGG - Chr4:1560729-1560748 MsG0480018207.01.T01:intron 55.0%
!! CTGCTGTTTCGCAGCTCTGT+TGG - Chr4:1560569-1560588 MsG0480018207.01.T01:intron 55.0%
!! GTTCATTGGGAGTGTTGGGG+TGG - Chr4:1560269-1560288 MsG0480018207.01.T01:intron 55.0%
!! TCAAGGTGTTGTCGTGGCAG+TGG - Chr4:1560424-1560443 MsG0480018207.01.T01:intron 55.0%
!!! CGGTTGGTGGGGTTGTGTTT+TGG - Chr4:1560745-1560764 MsG0480018207.01.T01:intron 55.0%
AAGGAGGAGAGTGATCGCGG+CGG + Chr4:1558704-1558723 None:intergenic 60.0%
ATGCACAGGGGTAGTGAGGC+AGG - Chr4:1560838-1560857 MsG0480018207.01.T01:intron 60.0%
GAAGATGGAGGAGGATGCGG+CGG + Chr4:1558739-1558758 None:intergenic 60.0%
GCCATTCCCGACGGCACATT+GGG + Chr4:1562433-1562452 None:intergenic 60.0%
GTGGTCGCGTTGCTTGGTCT+TGG + Chr4:1558656-1558675 None:intergenic 60.0%
GTTGTTAAGCCGGGTGAGGG+GGG - Chr4:1558923-1558942 MsG0480018207.01.T01:intron 60.0%
TGCACAGGGGTAGTGAGGCA+GGG - Chr4:1560839-1560858 MsG0480018207.01.T01:intron 60.0%
TGCCATTCCCGACGGCACAT+TGG + Chr4:1562434-1562453 None:intergenic 60.0%
TGGAGAGTGGTCGCGTTGCT+TGG + Chr4:1558662-1558681 None:intergenic 60.0%
! AGCTCTGTTGGCTGGTGGCT+GGG - Chr4:1560581-1560600 MsG0480018207.01.T01:intron 60.0%
! TTCGCAGCTCTGTTGGCTGG+TGG - Chr4:1560576-1560595 MsG0480018207.01.T01:intron 60.0%
!! ATTGGGAGTGTTGGGGTGGG+AGG - Chr4:1560273-1560292 MsG0480018207.01.T01:intron 60.0%
!! GCGGTGATGGATGGAGAAGG+AGG + Chr4:1558720-1558739 None:intergenic 60.0%
!! GGTGGTGGATGGATGGAGAG+TGG + Chr4:1558675-1558694 None:intergenic 60.0%
!! GTCGTGTGGTGGTGGATGGA+TGG + Chr4:1558682-1558701 None:intergenic 60.0%
!! TTGGGAGTGTTGGGGTGGGA+GGG - Chr4:1560274-1560293 MsG0480018207.01.T01:intron 60.0%
ACTGAGCGGGCAGTCATCCC+CGG + Chr4:1561559-1561578 None:intergenic 65.0%
GCACAGGGGTAGTGAGGCAG+GGG - Chr4:1560840-1560859 MsG0480018207.01.T01:intron 65.0%
GCCCAATGTGCCGTCGGGAA+TGG - Chr4:1562429-1562448 MsG0480018207.01.T01:five_prime_UTR 65.0%
! CAGCTCTGTTGGCTGGTGGC+TGG - Chr4:1560580-1560599 MsG0480018207.01.T01:intron 65.0%
!! GAGGATGCGGCGGTGATGGA+TGG + Chr4:1558729-1558748 None:intergenic 65.0%
!! GCGGCGGTGATGGATGGAGA+AGG + Chr4:1558723-1558742 None:intergenic 65.0%
CTGAGCGGGCAGTCATCCCC+GGG + Chr4:1561558-1561577 None:intergenic 70.0%
GAGTGATCGCGGCGGTCGTG+TGG + Chr4:1558696-1558715 None:intergenic 70.0%
GGAGGAGGATGCGGCGGTGA+TGG + Chr4:1558733-1558752 None:intergenic 70.0%
GTGAGGGGGGAGTTGCCGGT+GGG - Chr4:1558936-1558955 MsG0480018207.01.T01:intron 70.0%
TGATCGCGGCGGTCGTGTGG+TGG + Chr4:1558693-1558712 None:intergenic 70.0%
!! GGCGGTCGTGTGGTGGTGGA+TGG + Chr4:1558686-1558705 None:intergenic 70.0%
CCGGCAACTCCCCCCTCACC+CGG + Chr4:1558935-1558954 None:intergenic 75.0%
CCGGGTGAGGGGGGAGTTGC+CGG - Chr4:1558932-1558951 MsG0480018207.01.T01:intron 75.0%
GGTGAGGGGGGAGTTGCCGG+TGG - Chr4:1558935-1558954 MsG0480018207.01.T01:intron 75.0%
TCGCGGCGGTCGTGTGGTGG+TGG + Chr4:1558690-1558709 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 1558635 1562459 1558635 ID=MsG0480018207.01;Name=MsG0480018207.01
Chr4 mRNA 1558635 1562459 1558635 ID=MsG0480018207.01.T01;Parent=MsG0480018207.01;Name=MsG0480018207.01.T01;_AED=0.44;_eAED=0.59;_QI=229|0.25|0.6|1|0|0|5|0|124
Chr4 exon 1558635 1558780 1558635 ID=MsG0480018207.01.T01:exon:985;Parent=MsG0480018207.01.T01
Chr4 exon 1559518 1559636 1559518 ID=MsG0480018207.01.T01:exon:984;Parent=MsG0480018207.01.T01
Chr4 exon 1560233 1560248 1560233 ID=MsG0480018207.01.T01:exon:983;Parent=MsG0480018207.01.T01
Chr4 exon 1561498 1561625 1561498 ID=MsG0480018207.01.T01:exon:982;Parent=MsG0480018207.01.T01
Chr4 exon 1562265 1562459 1562265 ID=MsG0480018207.01.T01:exon:981;Parent=MsG0480018207.01.T01
Chr4 five_prime_UTR 1562265 1562459 1562265 ID=MsG0480018207.01.T01:five_prime_utr;Parent=MsG0480018207.01.T01
Chr4 five_prime_UTR 1561592 1561625 1561592 ID=MsG0480018207.01.T01:five_prime_utr;Parent=MsG0480018207.01.T01
Chr4 CDS 1561498 1561591 1561498 ID=MsG0480018207.01.T01:cds;Parent=MsG0480018207.01.T01
Chr4 CDS 1560233 1560248 1560233 ID=MsG0480018207.01.T01:cds;Parent=MsG0480018207.01.T01
Chr4 CDS 1559518 1559636 1559518 ID=MsG0480018207.01.T01:cds;Parent=MsG0480018207.01.T01
Chr4 CDS 1558635 1558780 1558635 ID=MsG0480018207.01.T01:cds;Parent=MsG0480018207.01.T01
Gene Sequence

>MsG0480018207.01.T01

ATGAACTTGAACAATTTTGATGGCGGCCACCCCAAACTTTTTTGTCTGGCTCCGCCACTGATTCACATCACAGAGATGAAAGTGAAAATCTTGCGGGTAGTGAGGCAGGGATGGCATGCTGTTGCTTCATGGACTTGGGATGCACAAGATGAAACATGTGGGATTTGTAGGATGGCCTTTGATGGATGTTGTCCTGATTGTAAACTTCCCGGGGATGACTGCCCGCTCATATTTAGTAGTCTAACTCTTTTTCCTACTACTTGCAGTTTGGGGTGCATGCAATCATGCATTTCATTTGCATTGTATCTTGAAATGGGTGAATTCTCAGACATCACAAGCACATTGCCCAATGTGCCGTCGGGAATGGCAATTTAA
Protein sequence

>MsG0480018207.01.T01

MNLNNFDGGHPKLFCLAPPLIHITEMKVKILRVVRQGWHAVASWTWDAQDETCGICRMAFDGCCPDCKLPGDDCPLIFSSLTLFPTTCSLGCMQSCISFALYLEMGEFSDITSTLPNVPSGMAI*